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Brandon Aguiar completed his Bachelor degree in Chemistry in 2016 at the University of Porto, Faculty of Sciences under the guidance of Prof. Fernanda Borges and his M.SC degree in Analytical, Clinical and Forensic Toxicology at the University of Porto, Faculty of Pharmacy under the guidance of Prof. Isabel Almeida and Prof. Helena Carmo. While working in his Bachelor's degree project " Development of new antioxidants compounds using natural models" Brandon was responsible for synthesize, purify and characterize by Nuclear Magnetic Resonance spectra (1H, 13C NMR and DEPT) new antioxidants based on ferulic acid scaffold and later evaluate their antioxidant activity using DPPH and ABTS radicals assay. During his Master's thesis Brandon was responsible for obtaining and analyzing UV/VIS spectra and implementing a ROS generation protocol to evaluate photoreactivity of chemicals by spectrophotometric monitoring the generation of singlet oxygen and superoxide anion. He also acquired experience in statistical analysis using One-way ANOVA to interpret the obtained results and became especially proficient in in vitro cell methodologies including cytotoxicity and phototoxicity evaluation using a neutral red assay in the HaCat cell line. He was also responsible for organizing and planning experimental work and for lab maintenance. In 2019, Brandon worked in a research project focused on the development of centrally-active and safe catechol O-methyltransferase inhibitors where he was responsible for synthesize, purify and characterize by Nuclear Magnetic Resonance spectra (1H, 13C NMR and DEPT) new antioxidants based on kojic acid and deferiprone scaffolds. While working on this project he developed very crucial skills in organic synthesis such as Microwave-assisted Cu-Catalyzed Protodecarboxylation of aromatic carboxylic acids, Jones Oxidation, protection of reactive groups, amidations and various purification techniques such as liquid-liquid extraction, filtration under reduced pressure and purifications through column chromatography on silica gel by classical method and by Biotage IsoleraTM Prime. In 2020 Brandon worked as a research fellow under the guidance of Prof. Fernanda Borges in the project: Redox-active phytochemicals triggering mitochondrial hormesis: developing a new generation of cosmetic active ingredients where he further improved his skills in organic synthesis, purification and characterization. Currently, Brandon is working on his PhD in Pharmaceutical Sciences with a scholarship funded by FCT in the project intilted: ROS-sensitive nanoparticles as carriers for Edaravone delivery: an innovative strategy in Amyotrophy Lateral Sclerosis treatment. In his PhD, Brandon acquired experience in the synthesis and characterization (DLS, HPLC, Release Studies, ABTS assay) of polymeric, hybrid and lipidic nanoparticles as well as in cell culture (SH-SY5Y, HepG2, NSC-34 and Caco-2 cell line) and in In vitro studies including Neutral Red, Razasorine, ROS and sulforhodamine B (SRB) assay.
Identificação

Identificação pessoal

Nome completo
Brandon Lage Aguiar

Nomes de citação

  • Brandon Aguiar

Identificadores de autor

Ciência ID
0415-57D5-A7C7
ORCID iD
0000-0002-8112-7550

Domínios de atuação

  • Ciências Médicas e da Saúde - Medicina Básica - Toxicologia
  • Ciências Médicas e da Saúde - Medicina Básica - Química Médica
  • Ciências Exatas - Química - Química Orgânica
  • Ciências Naturais - Ciências Biológicas - Biologia Celular
  • Ciências da Engenharia e Tecnologias - Nanotecnologia
  • Ciências Exatas - Química - Ciência de Polímeros

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1)
Formação
Grau Classificação
2021/01/01 - 2024/01/01
Em curso
Doutoramento em Ciências Farmacêuticas (Doutoramento)
Universidade do Porto Faculdade de Farmácia, Portugal
2016/09 - 2018/11
Concluído
Analytical, Clinical and Forensic Toxicology (Mestrado)
Universidade do Porto Faculdade de Farmácia, Portugal
"Evaluation of the phototoxicity of topical phenolic antioxidants" (TESE/DISSERTAÇÃO)
16
2013/09 - 2016/08
Concluído
Química (Licenciatura)
Universidade do Porto Faculdade de Ciências, Portugal
"Development of new antioxidants compounds using natural models" (TESE/DISSERTAÇÃO)
14
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2021/01/01 - 2021/06/30 Estagiário de Investigação (Investigação) Universidade do Porto Faculdade de Farmácia, Portugal
Universidade do Porto Faculdade de Farmácia, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2019/12/31 - 2020/05/31 Research fellow Universidade do Porto Faculdade de Ciências, Portugal
Universidade do Porto Faculdade de Ciências, Portugal
Projetos

Projeto

Designação Financiadores
2021/01/01 - Atual ROS-sensitive nanoparticles as carriers for Edaravone delivery: an innovative strategy in Amyotrophy Lateral Sclerosis treatment
2020.08731.BD
Bolseiro de Doutoramento
Fundação para a Ciência e a Tecnologia
Em curso
2019/12/01 - 2020/05/31 Redox-active phytochemicals triggering mitochondrial hormesis: developing a new generation of cosmetic active ingredients
POCI-01-0145-FEDER-028607
Investigador
Universidade do Porto Faculdade de Ciências, Portugal
Concluído

Outro

Designação Financiadores
2019/02/01 - 2019/11/30 COMT4BRAIN: Development of centrally-active and safe catechol O-methyltransferase inhibitors
POCI-01-0145-FEDER-029164
Outra
Universidade do Porto Faculdade de Ciências, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
Produções

Publicações

Artigo em revista
  1. Aguiar, Brandon; Carmo, Helena; Garrido, Jorge; Sousa Lobo, José M.; Almeida, Isabel F.. "In Vitro Evaluation of the Photoreactivity and Phototoxicity of Natural Polyphenol Antioxidants". Molecules 27 1 (2021): 189. http://dx.doi.org/10.3390/molecules27010189.
    Publicado • 10.3390/molecules27010189
  2. Chavarria; Fernandes; Aguiar; Silva; Garrido; Remião; Oliveira; Uriarte; Borges. "Insights into the Discovery of Novel Neuroprotective Agents: A Comparative Study between Sulfanylcinnamic Acid Derivatives and Related Phenolic Analogues". Molecules 24 23 (2019): 4405. http://dx.doi.org/10.3390/molecules24234405.
    Aceite para publicação • 10.3390/molecules24234405
Poster em conferência
  1. Brandon Aguiar. "Evaluation of Physicochemical features and biological activity of propargylamine-containing compounds for Parkinson’s disease". Trabalho apresentado em International Symposium on Advances in Synthetic and Medicinal Chemistry, 2023.
  2. Brandon Aguiar. "Fine-tuning the reactivity and drug-like profile of potential covalent NOX2 inhibitors". Trabalho apresentado em XII Meeting of the Paul Ehrlich Euro-PhD Network, Thessaloniki, 2023.
Resumo em conferência
  1. Brandon Aguiar. "Development of polymeric nanoparticles to ameliorate catechol O-methyltransferase Inhibitor induced hepatotoxicity". Trabalho apresentado em Encontro Nacional de jovens investigadores da UP, IJUP23, Porto, 2023.
    Publicado
  2. Brandon Aguiar. "Design and optimization of Edaravone loaded PLGA-based nanoparticles". Trabalho apresentado em XVI Encontro de Jovens Investigadores da UP (IJUP), 2023, Porto, 2023.
    Publicado
  3. Brandon Aguiar. "In vitro evaluation of the phototoxicity of natural phenolic antioxidants". Trabalho apresentado em SPF Meeting 2019, Porto, 2019.
    Publicado
  4. Brandon Aguiar. "In vitro evaluation of the photoreactivity of natural phenolic antioxidants". Trabalho apresentado em XXIV Encontro Luso Galego de Química, Porto, 2018.
    Publicado
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2019/02/06 In vitro evaluation of the phototoxicity of natural phenolic antioxidants XLIX REUNIÃO SOCIEDADE PORTUGUESA DE FARMACOLOGIA, XXXVII REUNIÃO FARMACOLOGIA CLÍNICA, XVIII REUNIÃO TOXICOLOGIA
(Porto , Portugal)
2018/11/21 “In vitro evaluation of the photoreactivity of natural phenolic antioxidants XXIV Encontro LusoGalego de Química 21-23 Nov Porto-Portugal
(Porto, Portugal)

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2016/03/17 - 2016/03/20 14ª Mostra UP I exercised the functions of demonstrator of chemistry and biochemistry experiments for high school students. (2016/03/17 - 2016/03/20)
Exposição
Universidade do Porto Faculdade de Ciências, Portugal
2015/04/11 - 2015/04/11 Organization of the "Semifinal das Olimpíadas de Química Junior 2015" (2015/04/11 - 2015/04/11) Universidade do Porto Faculdade de Ciências, Portugal