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The PI Carlos Frazão leads the ITQB-NOV Structural Biology Laboratory aiming the understanding of biochemical processes at atomic level, mainly based on the determination of biological macromolecules 3D structures using X-rays diffraction analysis. The interpretation of these structures originates clues on how biomolecules may interact, and has enabled the rationalization of applications in diverse fields of life sciences. The PI promotes the collaborations with other research groups to study key biological macromolecules, like proteins and complexes between proteins and nucleic-acids. The scope of developed projects includes electrons transfer meatllo proteins like iron sulfur proteins, mono and multi-heme cytochromes, hybrid cluster and Rieske proteins, flavo di-iron proteins, heme catalese, sulfur oxygenase-reductase, persulfide dioxigenase as well as various enzymes like the messenger RNA control ribonuclease II, aspartic or cysteine proteases, amidase, manganese superoxide dismutase, poly-saccharide synthesis enzymes, osteocalcine or directed evolution improved enzymes, among other examples.
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Identificação

Identificação pessoal

Nome completo
Carlos Maria de Noronha Galvão Franco Frazão
Data de nascimento
1959/04/04
Género
Masculino

Nomes de citação

  • Frazão, Carlos
  • Carlos Frazao

Identificadores de autor

Ciência ID
7B13-8949-DA58
ORCID iD
0000-0002-7725-1671

Endereços de correio eletrónico

  • frazao@itqb.unl.pt (Profissional)

Telefones

Telefone
  • (351) 21 4469666 (Profissional)

Moradas

  • Av. da República, 2780-157, Oeiras, Oeiras, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Naturais - Outras Ciências Naturais
  • Ciências Naturais - Outras Ciências Naturais

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C1) Utilizador proficiente (C2) Utilizador proficiente (C1)
Francês Utilizador independente (B2) Utilizador proficiente (C1) Utilizador independente (B1) Utilizador proficiente (C1) Utilizador independente (B1)
Alemão Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B2) Utilizador elementar (A2)
Espanhol; Castelhano Utilizador independente (B2) Utilizador elementar (A2) Utilizador proficiente (C1) Utilizador elementar (A2)
Formação
Grau Classificação
1984/10/01 - 1988/07/27
Concluído
 dotoramento (Doktor (PhD))
Especialização em Química
Die Fakultaet fuer Chemie, Biologie und Geowissenshaften der Technischen Universitaet Muenchen verleiht, Alemanha
"Synthese und Struktur von Silanen und Phosphanen mit verzweigtkettigen und ungesaettigten Substituenten" (TESE/DISSERTAÇÃO)
 Sehr gut bestanden
1978/10/01 - 1983/12/17
Concluído
 Engenharia Qquímica (Mestrado)
Instituto Superior Técnico, Universidade Técnica de Lisboa, Portugal
 16
1977/01 - 1979/05/01
Concluído
 Técnioco de Laboratório (Outros)
Especialização em Técnioco de Laboratório
Laboratórios Fidelis S.A.R.L, Portugal
 n. a.
1972/10/01 - 1976/09/10
Concluído
 Alínia F (Ensino secundário)
Liceu Nacional de D. Pedro V, Portugal
 18
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2008/02/01 - Atual Investigador principal (carreira) (Investigação) Universidade Nova de Lisboa, Portugal
1995/11/01 - 2008/02/01 Assistente de Investigação (carreira) (Investigação) Centro de Tecnologia Química e Biológica, Portugal
Centro de Tecnologia Química e Biológica, Portugal
1992/10/01 - 1995/10/01 Investigador Auxiliar (carreira) (Investigação) Universidade Nova de Lisboa, Portugal
1990/10/01 - 1992/10/01 Assistente de Investigação (carreira) (Investigação) Centro de Tecnologia Química e Biológica, Portugal
1990/03/01 - 1990/09/01 Investigador visitante (Investigação) Birkbeck College, University of London, Reino Unido
Birkbeck College, University of London, Reino Unido
1988/09/01 - 1990/02/01 Pós-doutorado (Investigação) Birkbeck College, University of London, Reino Unido

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento 		Empregador
1984/03/01 - 1984/07/01 Assistente (Docente Universitário) Instituto Superior Técnico, Universidade Técnica de Lisboa, Portugal
Projetos

Projeto

Designação Financiadores
2021/01/01 - 2023/12/31 Como é a resposta microbiana ao óxido nítrico regulada por fosforilação
PTDC/BIA-BQM/0562/2020
Universidade Nova de Lisboa, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2018/10/12 - 2021/10/11 Evolução Laboratorial e Computacional Iterativa de Enzimas Bacteriana Lignolíticas
PTDC/BII-BBF/29564/2017
Universidade Nova de Lisboa, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2018/10/01 - 2021/09/30 Diversidade e complexidadde das proteínas diférricas microbianas com multidomínios redutoras de oxigénio e óxido nítrico de Clostridiales
PTDC/BIA-BQM/27959/2017
Universidade Nova de Lisboa, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2016/05/01 - 2019/10/31 Análise estrutural e funcional do factor de transcrição Haa1 indispensável na resposta e resistência da levedura a ácido acético
PTDC/BBB-BEP/0385/2014
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2016/05/01 - 2019/10/31 Proteínas diférricas na resposta microbiana a stress oxidativo e nitrosativo
PTDC/BBB-BQB/3135/2014
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
2016/03/01 - 2019/02/28 BIOCATÁLISE E TECNOLOGIA DE ENZIMAS LENHINOLÍTICAS
PTDC/BBB-EBB/0122/2014
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2011/01/01 - 2014/06/30 hCE-2 - expressão e caracterização em modelos in vitro e in silico
PTDC/EBB-BIO/111530/2009
Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2010/01/01 - 2013/10/31 Projecto Estrutural sobre a Metalómica de Deinococcus radiodurans
PTDC/BIA-PRO/100365/2008
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
2010/04/01 - 2013/09/30 BIOMYR: Em direcção à engenharia metabólica da via do beta-mirceno de Pseudomonas sp. M1: abordagens de genómica funcional e bioquímica estrutural
PTDC/EBB-BIO/104980/2008
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal

Universidade do Minho, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2010/01/01 - 2013/06/30 Explorando a fosfomanose isomerase do tipo II BceAJ como alvo para o desenvolvimento de novos antimicrobianos e para aplicações biotechnológicas
PTDC/EBB-BIO/098352/2008
Universidade de Lisboa Instituto Superior Técnico, Portugal

Instituto de Biotecnologia e Bioengenharia, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2008/01/01 - 2011/03/31 Estudos Bioquímicos e Estruturais em UDP-Glucose Desidrogenases
PTDC/QUI/67925/2006
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2005/07/01 - 2007/06/30 Pesquisa de proteínas hiperstáveis em proteomas de hipertermófilos
POCI/BIO/58465/2004
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
2005/06/01 - 2007/06/30 Caracterização estrutural de proteínas membranares da cadeia respiratória de um organismo termoacidofílico
POCI/BIA-PRO/55621/2004
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em conferência
  1. Borges, Patricia T.; Joana Rocha; Alma Popescu; Dalila Mil-Homens; Isabel Sá-Correia; Arsénio Fialho; Carlos Frazao. "Hydrolysis of the thioester intermediate in UDP-glucose dehydrogenases". Trabalho apresentado em XXII Congress and General Assembly, International Union of Crystallography (IUCr), 2011.
Artigo em revista
  1. Brissos, Vânia; Borges, Patrícia T.; Sancho, Ferran; Lucas, Maria Fátima; Frazão, Carlos; Conzuelo, Felipe; Martins, Lígia O.. "Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases". JBIC Journal of Biological Inorganic Chemistry (2024): http://dx.doi.org/10.1007/s00775-023-02040-y.
    10.1007/s00775-023-02040-y
  2. Frade, Kelly; Silveira, Célia M.; Salgueiro, Bruno A.; Mendes, Sónia; Martins, Lígia O.; Frazão, Carlos; Todorovic, Smilja; Moe, Elin. "Biochemical, Biophysical, and Structural Analysis of an Unusual DyP from the Extremophile Deinococcus radiodurans". Molecules 29 2 (2024): 358. http://dx.doi.org/10.3390/molecules29020358.
    10.3390/molecules29020358
  3. Taborda, André; Frazão, Tomás; Rodrigues, Miguel V.; Fernández-Luengo, Xavier; Sancho, Ferran; Lucas, Maria Fátima; Carlos Frazao; et al. "Mechanistic insights into glycoside 3-oxidases involved in C-glycoside metabolism in soil microorganisms". Nature Communications 14 1 (2023): http://dx.doi.org/10.1038/s41467-023-42000-3.
    10.1038/s41467-023-42000-3
  4. Brissos, Vânia; Borges, Patricia T.; Ferran Sancho; Maria Fátima Lucas; Carlos Frazao; Felipe Conzuelo; Martins, Lígia. "Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases". Journal of Biological Inorganic Chemistry. Accepted (2023):
    Aceite para publicação
  5. Brissos, Vânia; Borges, Patrícia T.; Núñez-Franco, Reyes; Lucas, Maria Fátima; Carlos Frazao; Monza, Emanuele; Masgrau, Laura; Cordeiro, Tiago N.; Martins, Lígia O.. "Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase". ACS Catalysis 12 9 (2022): 5022-5035. http://dx.doi.org/10.1021/acscatal.2c00336.
    10.1021/acscatal.2c00336
  6. Borges, Patrícia T.; Silva, Diogo; Silva, Tomás F.D.; Brissos, Vânia; Cañellas, Marina; Lucas, Maria Fátima; Masgrau, Laura; et al. "Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase". Computational and Structural Biotechnology Journal 20 (2022): 3899-3910. http://dx.doi.org/10.1016/j.csbj.2022.07.032.
    10.1016/j.csbj.2022.07.032
  7. Rodrigues, Carolina F.; Borges, Patrícia T.; Scocozza, Magali F.; Silva, Diogo; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia O.. "Loops around the Heme Pocket Have a Critical Role in the Function and Stability of BsDyP from Bacillus subtilis". International Journal of Molecular Sciences 22 19 (2021): 10862. http://dx.doi.org/10.3390/ijms221910862.
    10.3390/ijms221910862
  8. Borges, Patrícia T.; Brissos, Vânia; Hernandez, Guillem; Masgrau, Laura; Lucas, Maria Fátima; Monza, Emanuele; Frazão, Carlos; Cordeiro, Tiago N.; Martins, Lígia O.. "Methionine-Rich Loop of Multicopper Oxidase McoA Follows Open-to-Close Transitions with a Role in Enzyme Catalysis". ACS Catalysis 10 13 (2020): 7162-7176. http://dx.doi.org/10.1021/acscatal.0c01623.
    Publicado • 10.1021/acscatal.0c01623
  9. Martins, Maria C.; Romão, Célia V.; Folgosa, Filipe; Borges, Patrícia T.; Frazão, Carlos; Teixeira, Miguel. "How superoxide reductases and flavodiiron proteins combat oxidative stress in anaerobes". Free Radical Biology and Medicine 140 (2019): 36-60. http://dx.doi.org/10.1016/j.freeradbiomed.2019.01.051.
    Publicado • 10.1016/j.freeradbiomed.2019.01.051
  10. Borges, Patrícia T.; Romão, Célia V.; Saraiva, Lígia M.; Gonçalves, Vera L.; Carrondo, Maria A.; Teixeira, Miguel; Frazão, Carlos. "Analysis of a new flavodiiron core structural arrangement in Flv1-¿FlR protein from Synechocystis sp. PCC6803". Journal of Structural Biology 205 1 (2019): 91-102. http://dx.doi.org/10.1016/j.jsb.2018.11.004.
    Publicado • 10.1016/j.jsb.2018.11.004
  11. Frade, Kelly Stefany Tuna; Fernandes, Andreia Cecília Pimenta; Silveira, Celia Marisa; Frazão, Carlos; Moe, Elin. "A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans: cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis". Acta Crystallographica Section F Structural Biology Communications 74 7 (2018): 419-424. http://dx.doi.org/10.1107/s2053230x18008488.
    10.1107/s2053230x18008488
  12. Frade, Kelly; Fernandes, Andreia; Silveira, Celia; Frazão, Carlos; Todorovic, Smilja; Moe, Elin. "Biophysical and crystallization analysis of a novel peroxidase from Deinococcus radiodurans". Free Radical Biology and Medicine 120 (2018): S115. http://dx.doi.org/10.1016/j.freeradbiomed.2018.04.379.
  13. Pereira, L.; Saraiva, I.H.; Oliveira, A.S.F.; Soares, C.M.; Louro, R.O.; Frazão, C.. "Molecular structure of FoxE, the putative iron oxidase of Rhodobacter ferrooxidans SW2". Biochimica et Biophysica Acta - Bioenergetics 1858 10 (2017): 847-853. http://www.scopus.com/inward/record.url?eid=2-s2.0-85027514977&partnerID=MN8TOARS.
    10.1016/j.bbabio.2017.07.002
  14. Romão, C.V.; Vicente, J.B.; Borges, P.T.; Frazão, C.; Teixeira, M.. "The dual function of flavodiiron proteins: Oxygen and/or nitric oxide reductases". Journal of Biological Inorganic Chemistry 21 1 (2016): 39-52. http://www.scopus.com/inward/record.url?eid=2-s2.0-84959364764&partnerID=MN8TOARS.
    10.1007/s00775-015-1329-4
  15. Romão, C.V.; Vicente, J.B.; Borges, P.T.; Victor, B.L.; Lamosa, P.; Silva, E.; Pereira, L.; et al. "Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase". Journal of Molecular Biology 428 23 (2016): 4686-4707. http://www.scopus.com/inward/record.url?eid=2-s2.0-84996538192&partnerID=MN8TOARS.
    10.1016/j.jmb.2016.10.008
  16. Gonc¸alves, V.L.; Vicente, J.B.; Pinto, L.; Romão, C.V.; Frazão, C.; Sarti, P.; Giuffrè, A.; Teixeira, M.. "Flavodiiron oxygen reductase from Entamoeba histolytica: Modulation of substrate preference by tyrosine 271 and lysine 53". Journal of Biological Chemistry 289 41 (2014): 28260-28270. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907638177&partnerID=MN8TOARS.
    10.1074/jbc.M114.579086
  17. Borges, P.T.; Frazão, C.; Miranda, C.S.; Carrondo, M.A.; Romão, C.V.. "Structure of the monofunctional heme catalase DR1998 from Deinococcus radiodurans". The FEBS journal 281 18 (2014): 4138-4150. http://www.scopus.com/inward/record.url?eid=2-s2.0-84909944927&partnerID=MN8TOARS.
    10.1111/febs.12895
  18. Marques, A.T.; Santos, S.P.; Rosa, M.G.; Rodrigues, M.A.A.; Abreu, I.A.; Frazão, C.; Romão, C.V.. "Expression, purification and crystallization of MnSOD from Arabidopsis thaliana". Acta Crystallographica Section F:Structural Biology Communications 70 5 (2014): 669-672. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905435432&partnerID=MN8TOARS.
    10.1107/S2053230X14007687
  19. Borges, P.T.; Miranda, C.S.; Santos, S.P.; Carita, J.N.; Frazão, C.; Romão, C.V.. "Purification, crystallization and phase determination of the DR1998 haem b catalase from Deinococcus radiodurans". Acta Crystallographica Section F:Structural Biology Communications 70 5 (2014): 659-662. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905447633&partnerID=MN8TOARS.
    10.1107/S2053230X1400764X
  20. Bandeiras, T.M.; Freitas, M.C.; Petrasch, D.; Kletzin, A.; Frazão, C.. "SAD phasing towards structure determination of a thermostable Rieske ferredoxin with a novel stabilizing disulfide bridge". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69 5 (2013): 555-558. http://www.scopus.com/inward/record.url?eid=2-s2.0-84882342812&partnerID=MN8TOARS.
    10.1107/S1744309113008385
  21. Barbas, A.; Popescu, A.; Frazão, C.; Arraiano, C.M.; Fialho, A.M.. "Rossmann-fold motifs can confer multiple functions to metabolic enzymes: RNA binding and ribonuclease activity of a UDP-glucose dehydrogenase". Biochemical and Biophysical Research Communications 430 1 (2013): 218-224. http://www.scopus.com/inward/record.url?eid=2-s2.0-84872422260&partnerID=MN8TOARS.
    10.1016/j.bbrc.2012.10.091
  22. Pereira, L.; Saraiva, I.H.; Coelho, R.; Newman, D.K.; Louro, R.O.; Frazão, C.. "Crystallization and preliminary crystallographic studies of FoxE from Rhodobacter ferrooxidans SW2, an FeII oxidoreductase involved in photoferrotrophy". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 68 9 (2012): 1106-1108. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865705261&partnerID=MN8TOARS.
    10.1107/S174430911203271X
  23. Rocha, J.; Popescu, A.O.; Borges, P.; Mil-Homens, D.; Moreira, L.M.; Sá-Correia, I.; Fialho, A.M.; Frazão, C.. "Structure of Burkholderia cepacia UDP-glucose dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate". Journal of Bacteriology 193 15 (2011): 3978-3987. http://www.scopus.com/inward/record.url?eid=2-s2.0-79960418213&partnerID=MN8TOARS.
    10.1128/JB.01076-10
  24. Veith, A.; Urich, T.; Seyfarth, K.; Protze, J.; Frazão, C.; Kletzin, A.. "Substrate pathways and mechanisms of inhibition in the sulfur oxygenase reductase of Acidianus ambivalens". Frontiers in Microbiology 2 MAR (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-84855953639&partnerID=MN8TOARS.
    10.3389/fmicb.2011.00037
  25. Rocha, J.; Popescu, A.O.; Sá-Correia, I.; Fialho, A.M.; Frazão, C.. "Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 66 3 (2010): 269-271. http://www.scopus.com/inward/record.url?eid=2-s2.0-77749274048&partnerID=MN8TOARS.
    10.1107/S1744309109053500
  26. Rocha, J.; Granja, A.T.; Sá-Correia, I.; Fialho, A.; Frazão, C.. "Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 66 1 (2009): 69-72. http://www.scopus.com/inward/record.url?eid=2-s2.0-75149191829&partnerID=MN8TOARS.
    10.1107/S174430910904929X
  27. Freixo, M.d.R.; Karmali, A.; Frazão, C.; Arteiro, J.M.. "Production of laccase and xylanase from Coriolus versicolor grown on tomato pomace and their chromatographic behaviour on immobilized metal chelates". Process Biochemistry 43 11 (2008): 1265-1274. http://www.scopus.com/inward/record.url?eid=2-s2.0-53249121475&partnerID=MN8TOARS.
    10.1016/j.procbio.2008.07.013
  28. Frazão, C.; Aragão, D.; Coelho, R.; Leal, S.S.; Gomes, C.M.; Teixeira, M.; Carrondo, M.A.. "Crystallographic analysis of the intact metal centres [3Fe-4S]1+/0 and [4Fe-4S]2+/1+ in a Zn2+-containing ferredoxin". FEBS Letters 582 5 (2008): 763-767. http://www.scopus.com/inward/record.url?eid=2-s2.0-39649092718&partnerID=MN8TOARS.
    10.1016/j.febslet.2008.01.041
  29. Aragão, D.; Mitchell, E.P.; Frazão, C.F.; Carrondo, M.A.; Lindley, P.F.. "Structural and functional relationships in the hybrid cluster protein family: Structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 Å resolution". Acta Crystallographica Section D: Biological Crystallography 64 6 (2008): 665-674. http://www.scopus.com/inward/record.url?eid=2-s2.0-45749152544&partnerID=MN8TOARS.
    10.1107/S0907444908009165
  30. Vicente, J.B.; Carrondo, M.A.; Teixeira, M.; Frazão, C.. "Structural Studies on Flavodiiron Proteins". Methods in Enzymology 437 (2008): 3-19. http://www.scopus.com/inward/record.url?eid=2-s2.0-47049115155&partnerID=MN8TOARS.
    10.1016/S0076-6879(07)37001-8
  31. Araga~o, David; Fialho, Arse´nio M.; Marques, Ana R.; Mitchell, Edward P.; Sa´-Correia, Isabel; Fraza~o, Carlos. "The Complex of Sphingomonas elodea ATCC 31461 Glucose-1-Phosphate Uridylyltransferase with Glucose-1-Phosphate Reveals a Novel Quaternary Structure, Unique among Nucleoside Diphosphate-Sugar Pyrophosphorylase Members". Journal of Bacteriology 189 12 (2007): 4520-4528. http://dx.doi.org/10.1128/jb.00277-07.
    10.1128/jb.00277-07
  32. Andrade, J.; Karmali, A.; Carrondo, M.A.; Frazão, C.. "Crystallization, diffraction data collection and preliminary crystallographic analysis of hexagonal crystals of Pseudomonas aeruginosa amidase". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 63 3 (2007): 214-216. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947303749&partnerID=MN8TOARS.
    10.1107/S1744309107005830
  33. Aragão, D.; Fialho, A.M.; Marques, A.R.; Mitchell, E.P.; Sá-Correia, I.; Frazão, C.. "The complex of Sphingomonas elodea ATCC 31461 glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique among nucleoside diphosphate-sugar pyrophosphorylase members". Journal of Bacteriology 189 12 (2007): 4520-4528. http://www.scopus.com/inward/record.url?eid=2-s2.0-34250343820&partnerID=MN8TOARS.
    10.1128/JB.00277-07
  34. Andrade, J.; Karmali, A.; Carrondo, M.A.; Frazão, C.. "Structure of amidase from Pseudomonas aeruginosa showing a trapped acyl transfer reaction intermediate state". Journal of Biological Chemistry 282 27 (2007): 19598-19605. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547120236&partnerID=MN8TOARS.
    10.1074/jbc.M701039200
  35. Aragão, D.; Marques, A. R.; Frazão, C.; Enguita, F. J.; Carrondo, M. A.; Fialho, A. M.; Sá-Correia, I.; et al. "Cloning, expression, purification, crystallization and preliminary structure determination of glucose-1-phosphate uridylyltransferase (UgpG) fromSphingomonas elodeaATCC 31461 bound to glucose-1-phosphate". Acta Crystallographica Section F Structural Biology and Crystallization Communications 62 9 (2006): 930-934. http://dx.doi.org/10.1107/s1744309106030107.
    10.1107/s1744309106030107
  36. McVey, C.E.; Amblar, M.; Barbas, A.; Cairrão, F.; Coelho, R.; Romáo, C.; Arraiano, C.M.; Carrondo, M.A.; Frazão, C.. "Expression, purification, crystallization and preliminary diffraction data characterization of Escherichia coli ribonuclease II (RNase II)". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 62 7 (2006): 684-687. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745684855&partnerID=MN8TOARS.
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  44. Victor, B.L.; Vicente, J.B.; Rodrigues, R.; Oliveira, S.; Rodrigues-Pousada, C.; Frazão, C.; Gomes, C.M.; et al. "Docking and electron transfer studies between rubredoxin and rubredoxin:oxygen oxidoreductase". Journal of Biological Inorganic Chemistry 8 4 (2003): 475-488. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037951339&partnerID=MN8TOARS.
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    10.1110/ps.31202
  47. Frazão, C.; Enguita, F.J.; Coelho, R.; Sheldrick, G.M.; Navarro, J.A.; Hervás, M.; De la Rosa, M.A.; Carrondo, M.A.. "Crystal structure of low-potential cytochrome c549 from Synechocystis sp. PCC 6803 at 1.21 Å resolution". Journal of Biological Inorganic Chemistry 6 3 (2001): 324-332. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034855073&partnerID=MN8TOARS.
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  49. Frazão, C.; Silva, G.; Gomes, C.M.; Matias, P.; Coelho, R.; Sieker, L.; Macedo, S.; et al. "Structure of a dioxygen reduction enzyme from Desulfovibrio gigas". Nature Structural Biology 7 11 (2000): 1041-1045. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033767236&partnerID=MN8TOARS.
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  50. Frazão, C.; Sieker, L.; Coelho, R.; Morais, J.; Pacheco, I.; Chen, L.; LeGall, J.; et al. "Crystallization and preliminary diffraction data analysis of both single and pseudo-merohedrally twinned crystals of rubredoxin oxygen oxidoreductase from Desulfovibrio gigas". Acta Crystallographica Section D: Biological Crystallography 55 8 (1999): 1465-1467. http://www.scopus.com/inward/record.url?eid=2-s2.0-13044249172&partnerID=MN8TOARS.
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  51. Frazão, C.; Bento, I.; Costa, J.; Soares, C.M.; Veríssimo, P.; Faro, C.; Pires, E.; Cooper, J.; Carrondo, M.A.. "Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp- containing aspartic proteinase from the flowers of Cynara cardunculus L.". Journal of Biological Chemistry 274 39 (1999): 27694-27701. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033600810&partnerID=MN8TOARS.
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  55. Frazão, C; Soares, CM; Carrondo, MA; Pohl, E; Dauter, Z; Wilson, KS; Hervés, M; et al. "Ab initio determination of the crystal structure of cytochrome c6 and comparison with plastocyanin". Structure 3 11 (1995): 1159-1169. http://dx.doi.org/10.1016/s0969-2126(01)00252-0.
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  56. Morais, J.; Palma, P.N.; Frazão, C.; Caldeira, J.; LeGall, J.; Moura, I.; Moura, J.J.G.; Carrondo, M.A.. "Structure of the tetraheme cytochrome from Desulfovibrio desulfuricans ATCC 27774: X-ray diffraction and electron paramagnetic resonance studies". Biochemistry 34 39 (1995): 12830-12841. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028838508&partnerID=MN8TOARS.
  57. Frazao, C.; Dias, J.M.; Matias, P.M.; Romao, M.J.; Carrondo, M.A.; Hervas, M.; Navarro, J.A.; De La Rosa, M.; Sheldrick, G.M.. "Cytochrome c6 from the green alga Monoraphidium braunii. Crystallization and preliminary diffraction studies". Acta Crystallographica - Section D Biological Crystallography 51 2 (1995): 232-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029132455&partnerID=MN8TOARS.
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  58. Frazao, Carlos; Topham, C.; Dhanaraj, Venugopal; Blundell, T. L.. "Comparative modelling of human renin: A retrospective evaluation of the model with respect to the X-ray crystal structure". Pure and Applied Chemistry 66 1 (1994): 43-50. http://dx.doi.org/10.1351/pac199466010043.
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  62. Pitts, J. E.; Dhanaraj, V.; Dealwis, C. G.; Mantafounis, D.; Nugent, P.; Orprayoon, P.; Cooper, J. B.; Newman, M.; Blundell, T. L.. "Multidisciplinary cycles for protein engineering: Site-directed mutagenesis and X-ray structural studies of aspartic proteinases". Scandinavian Journal of Clinical and Laboratory Investigation 52 sup210 (1992): 39-50. http://dx.doi.org/10.1080/00365519209104653.
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  65. Cooper, J.; Quail, W.; Frazao, C.; Foundling, S.I.; Blundell, T.L.; Humblet, C.; Lunney, E.A.; Lowther, W.T.; Dunn, B.M.. "X-ray crystallographic analysis of inhibition of endothiapepsin by cyclohexyl renin inhibitors". Biochemistry 31 35 (1992): 8142-8150. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026699837&partnerID=MN8TOARS.
  66. Newman, M.; Safro, M.; Frazao, C.; Khan, G.; Zdanov, A.; Tickle, I.J.; Blundell, T.L.; Andreeva, N.. "X-ray analyses of aspartic proteinases IV". Journal of Molecular Biology 221 4 (1991): 1295-1309. http://dx.doi.org/10.1016/0022-2836(91)90934-x.
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  72. Calhorda, M.J.; Carrondo, M.A.A.F.D.C.T.; Dias, A.R.; Frazão, C.F.; Martinho Simões, J.A.; Teixeira, C.; Hursthouse, M.B.. "Energetics of Metal-Sulfur Bonds in the Complexes M(¿5-C5H5)2(SC2H5)2 (M = Ti, W) and W(¿5-C5H5)2(SC6H5)2. Molecular Structure of Ti(¿5-C5H5)2(SC2H5)2". Inorganic Chemistry 27 14 (1988): 2513-2518. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001438704&partnerID=MN8TOARS.
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Capítulo de livro
  1. Vicente, João B.; Carrondo, Maria Arménia; Teixeira, Miguel; Frazão, Carlos. "Structural Studies on Flavodiiron Proteins". In Globins and Other Nitric Oxide-Reactive Proteins, Part B, 3-19. Elsevier, 2008.
    10.1016/s0076-6879(07)37001-8
  2. Frazão, Carlos; McVey, Colin E.; Carrondo, MA. "Determinação da Estrutura da Ribonuclease II: uma enzima importante no controlo da expressão génica.". In Annualia, editado por Colaço, Jorge. Lisboa, Portugal: Editorial Verbo, 2007.
    Publicado
  3. Carlos Frazao. "Molecular Recognition and Drug Design: The Structural Basis of Specificity of Human and Mouse Renins Defined by X-Ray Analysis of Peptide Inhibitor Complexes". Reino Unido, 1992.
Entrada de dicionário
  1. Vicente, João B; Carrondo, Maria Arménia; Teixeira, Miguel; Frazão, Carlos. "Flavodiiron Proteins: Nitric Oxide and/or Oxygen Reductases". In Encyclopedia of Inorganic and Bioinorganic Chemistry. John Wiley & Sons, Ltd, 2011. http://dx.doi.org/10.1002/9781119951438.eibc0645.
    10.1002/9781119951438.eibc0645
Livro
  1. Faro, C.; Ramalho-Santos, M.; Veríssimo, P.; Pissarra, J.; Frazão, C.; Costa, J.; Lin, X.-L.; Tang, J.; Pires, E.. Structural and functional aspects of cardosins. 1998.
Poster em conferência
  1. Borges, Patricia T.; Diogo Silva; Carlos Frazao; Martins, Lígia. "Thermostable variants ant their impact on activity of a dye-decolorizing peroxidase from Pseudomonas putida". Trabalho apresentado em UK-Japan meeting on dynamic and time-resolved crystallography, Leicester, UK, 2023.
  2. Masgrau, Laura; Taborda, André; Frazão, Tomás; Rodrigues, Miguel; Fernandez-luengo, Xavier; Sancho, Ferran; Frazão, Carlos; et al. "Combined experimental and computational study for the characterization of Pseudarthrobacter siccitolerans". Trabalho apresentado em EuroCarb21, 2023.
  3. Taborda, André; Frazão, Tomás; Rodrigues, Miguel; Fernandez-luengo, Xavier; Sancho, Ferran; Frazão, Carlos; Ventura, Rita; et al. "Unveiling the structural determinants for substrate recognition and catalysis of bacterial C-glycoside oxidases". Trabalho apresentado em BIOTRANS 2023, 2023.
  4. Lorena, Constança; Diogo Silva; Carlos Frazao; Martins, Lígia O.; Borges, Patricia T.. "Thermostabilizing strategies performed by two computational methods in a DyP type peroxidase from Pseudomonas putida". Trabalho apresentado em 16th International Symposium on Biocatalysis & Biotransformations (BIOTRANS), 2023.
  5. Borges, Patricia T.; Diogo Silva; Carlos Frazao; Martins, Lígia. "STRUCTURAL DETERMINANTS THAT IMPACT THE STABILITY AND ACTIVITY OF A DYEDECOLORIZING PEROXIDASE FROM PSEUDOMONAS PUTIDA MET94". Trabalho apresentado em 9 th meeting of ENURS 2023- Annual meeting of Portugues Synchrotron Users, Leiria, Portugal, 2023.
  6. Lorena, Constança; Diogo silva; Carlos Frazao; Martins, Lígia O.; Borges, Patricia T.. "Structural determinants behind the improved thermostability of a DyP type peroxidase variant obtained by computational methods". Trabalho apresentado em International School on Biological Crystallization, 2023.
  7. Taborda, André; Frazão, Tomás; Rodrigues, Miguel; Fernandez-luengo, Xavier; Sancho, Ferran; Frazão, Carlos; Masgrau, Laura; Borges, Patricia; Martins, Lígia. "Structure-Function Relationships in Pseudarthrobacter siccitolerans Bacterial Glycoside 3-Oxidase". Trabalho apresentado em Novel Enzymes 2023, 2023.
  8. Borges, Patricia T.; Brissos, Vânia; Carlos Frazao; Martins, Lígia. "Role of a flexible loop in the substrate selectivity of an engineered variant of hyperthermophilic multicopper oxidase McoP". Trabalho apresentado em 16th International Symposium on Biocatalysis and Biotransformations (Biotrans 2023), La Rochelle, France, 2023.
  9. Salgueiro, Bruno; Martins, Maria C.; Gragera, Marcos; Folgosa, Filipe; Carazo, José; Teixeira, Miguel; Carlos Frazao; Romão, Célia V.. "Structural analysis of tree distinct classes of Flavodiiron proteins obtain by X-ray Crystallography and Single-Particle Cryo-EM". Trabalho apresentado em 2nd National Crystallographic Meeting, 2022.
  10. Lorena, Constança; Silva, Diogo; Frazão, Carlos; Martins, Lígia O.; Borges, Patricia T.. Autor correspondente: Lorena, Constança. "Structural analysis of a computationally designed DyP-type peroxidase with improved thermostability". Trabalho apresentado em 2nd National Crystallographic Meeting, July 15-16, Lisbon, Portugal, 2022.
  11. Salgueiro, Bruno; Ribeiro, Ricardo; Hernandez, Guillem; Cordeiro, Tiago; Romão, Célia V.; Isabel Sá-Correia; Frazão, Carlos. "Haa1 transcription factor DNA binding domain structural analysis of two homologous proteins". Trabalho apresentado em 7th European Crystallographic School, 2022.
  12. Borges, Patricia T.; Silva, Diogo; Carlos Frazao; Martins, Lígia O.. "EVOLUTION OF STRUCTURAL PROPERTIES IN A DYE-DECOLORIZING PEROXIDASE FROM PSEUDOMONAS PUTIDA MET94". Trabalho apresentado em Oxizymes Conference, Siena, Italy, 2022., 2022.
  13. Frazão, Tomás; Taborda, André; Martins, Lígia O.; Carlos Frazao; Borges, Patricia T.. Autor correspondente: Borges, Patricia T.. "Structural insights into a bacterial pyranose 2-oxidase from Pseudoarthrobacter siccitolerans, a candidate enzyme for health diagnosis biosensors". Trabalho apresentado em European Federation of Biotechnology - Virtual Conference, 2021.
  14. Borges, Patricia T.; Carlos Frazao; Ligia O. Martins. "Structural insights into the evolution of a dye-decolorizing peroxidase from Pseudomonas putida". Trabalho apresentado em NextGenBiocat, 2021.
  15. Borges, Patricia T.; Vania Brissos; Guillem Hernandez; Laura Masgrau; Maria Fatima Lucas; Emanuele Monza; Carlos Frazao; Tiago N. Cordeiro; Lígia O. Martins. "Structural analysis of a laboratory evolution variant of hyperthermophilic Multicopper Oxidase McoA". Trabalho apresentado em Protein Engineering Congress USA, 2020.
  16. Vania Brissos; Borges, Patricia T.; Laura Masgrau; Maria Fatima Lucas; Emanuele Monza; Carlos Frazao; Tiago N. Cordeiro; Ligia O. Martins. "Protein Engineering, Structural and Computational Studies to Assess Substrate Specificity Determinants of Multicopper Oxidases". Trabalho apresentado em Protein Engineering Congress USA, 2020.
  17. Bruno Salgueiro; Folgosa, Filipe; Romao, Celia V; Hernandez, Guillem; Tiago Cordeiro; Frazão, Carlos; Teixeira, Miguel. "Biochemical and structural characterization of a new Flavorubredoxin from Photobacterium swingsii". Trabalho apresentado em BioMetals 2020, 2020.
  18. Martins, Maria C.; Salgueiro, Bruno; Folgosa, Filipe; Romão, Célia V.; Frazão, Carlos; Teixeira, Miguel. "Survival strategies of Clostridium difficile to fluctuating concentrations of oxygen". Trabalho apresentado em 1st TIMB3 (Twin to illuminate metals in biology and biocatalysis through biospectroscopy) Annual Meeting, 2019.
  19. Gras, Guillem; Hugo Monteiro; Cordeiro, Tiago; Frazão, Carlos; Salgueiro, Bruno. "Dissecting the Structure of the N-Terminal Cytosolic Tail of the Translocated Intimin Receptor". Trabalho apresentado em PT NMR Satellite Meeting to SMASH, 2019.
    10.1063/1.58449
  20. Salgueiro, Bruno; Martins, Maria C.; Folgosa, Filipe; Romão, Célia; Teixeira, Miguel; Frazão, Carlos. "Crystal structures determination of classes A and F flavodiiron proteins from Clostridium difficile". Trabalho apresentado em 8th Meeting of Portuguese Synchrotron Radiation Users (ENURS 2019), 2019.
  21. Salgueiro, Bruno; Ribeiro, R.; Romão, C.V.; Hernandez, Guillem; Cordeiro, TN; Sá-Correia, I; Frazão, Carlos. "Haa1 transcription fator DNA binding domain structural analysis of two homologous proteins". Trabalho apresentado em I National Crystallography Meeting, 2019.
  22. Monteiro, Hugo; Vieira, Marta; Hernandez, Guillem; González, Amaia; Borges, Patrícia; Frazão, Carlos; Monza, Emanuele; et al. "Dynamic Strucutral Studies ¿ From Pathogenic Disordered Proteins To Folded Relevant Enzymes". Trabalho apresentado em Twin to Illuminate Metals in Biology and Biocatalysis through Biospectroscopy (TIMB3), 2018.
  23. Vila Santa, A; Moita, MR; Frazão, Carlos; Henriques, Sílvia F.; Sá-Correia, Isabel; Jarboe, LR; Vinga, Susana; Mira, NP. "Exploring a molecular systems biology approach to setup Saccharomyces cerevisiae as a cell factory for the production of itaconic acid". Trabalho apresentado em 32nd International Specialized Symposium in Yeasts, Perugia, 2015.
  24. Bandeiras, T; Freitas, MC; Petrasch, D; Botelho, Hugo M; Gomes, Cláudio M; Kletzin, A; Frazão, Carlos. "Purification, crystallization and preliminary crystallographic analysis of Acidianus ambivalens Rieske ferredoxin 2 (RFd2), a new type of Rieske ferredoxins with an additional disulfide". Trabalho apresentado em 1° Encontro Nacional de Utilizadores de Radiação de Sincrotrão, 2012.
  25. Bandeiras, T; Freitas, MC; Botelho, Hugo M; Gomes, Cláudio M; Carrondo, MA; Kletzin, A; Frazão, Carlos. "Crystallographic structure of Acidianus ambivalens Rieske ferredoxin 2 ¿ The disulfide bridge structural role". Trabalho apresentado em EMBO Practical Course: High-throughput methods for Protein Production and Crystallization, 2011.
  26. McVey, Colin E.; Barbas, Ana; Amblar, M.; Frazão, Carlos; Arraiano, C.M.; Carrondo, MA. "Crystallisation of and crystallographic analysis of RNase II from E. coli.". Trabalho apresentado em Protein Society Meeting, Barcelona, 2005.
Recurso online
  1. Borges, Patricia T.; Brissos, Vânia; Carlos Frazao; Martins, Lígia O.. Crystal structure of a multicopper oxidase 3F3 variant from Pyrobaculum aerophilum. 2024. https://www.rcsb.org/structure/unreleased/8P4G.
  2. Borges, Patricia T.; Frazao, Tomas; Taborda, André; Frazão, Carlos; Martins, Lígia O.. Crystal structure of a bacterial pyranose 2-oxidase in complex with mangiferin. 2023. https://www.rcsb.org/structure/7QVA.
  3. Borges, Patricia T.; Frazao, Tomas; Taborda, André; Frazão, Carlos; Martins, Lígia O.. Crystal structure of a bacterial pyranose 2-oxidase complex with D-glucose. 2023. https://www.rcsb.org/structure/7QFD.
  4. Borges, Patricia T.; Frazao, Tomas; Taborda, André; Carlos Frazao; Martins, Lígia O.. Crystal structure of a bacterial pyranose 2-oxidase from Pseudoarthrobacter siccitolerans. 2023. https://www.rcsb.org/structure/7QF8.
  5. Borges, Patricia T.; Martins, Lígia; Frazão, Carlos. Crystal structure of a DyP-type peroxidase 29E4 variant from Pseudomonas putida. 2022. https://www.rcsb.org/structure/7QZA.
  6. Borges, Patricia T.; Silva, D.; Martins, Lígia; Carlos Frazao. Crystal structure of a DyP-type peroxidase from Pseudomonas putida. 2022. https://www.rcsb.org/structure/7QYQ.
  7. Borges, Patricia T.; Silva, D.; Martins, Lígia; Frazão, Carlos. Crystal structure of a DyP-type peroxidase 6E10 variant from Pseudomonas putida. 2022. https://www.rcsb.org/structure/7QYZ.
  8. Borges, Patricia T.; Rodrigues, C..; Silva, D.,; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia. Crystal structure of a DyP-type peroxidase 5G5 variant from Bacillus subtilis. 2021. https://www.rcsb.org/structure/7PL0.
  9. Borges, Patricia T.; Rodrigues, C..; Silva, D..; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia. Crystal structure of a DyP-type peroxidase from Bacillus subtilis in P3121 space group. 2021. https://www.rcsb.org/structure/7PKX.
  10. Borges, Patricia T.; Brissos, Vânia; Cordeiro, Tiago N.; Martins, Lígia; Carlos Frazao. Crystal structure of McoA multicopper oxidase 2F4 variant from the hyperthermophile Aquifex aeolicus. 2021. https://www.rcsb.org/structure/6TTD.
Atividades

Orientação

Título / Tema
Papel desempenhado 		Curso (Tipo)
Instituição / Organização
2017/02 - Atual Licenciatura em Bioquímica
Orientador
 Licenciatura (Licenciatura/Bacharelato)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018/07 - 2019/10 Biocatalysis and Technology of Ligninolytic Enzymes (PTDC/BBB-EBB/0122/2014)
Orientador
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2012 - 2019/05/17 Unraveling structural features of flavodiiron proteins. A detailed structural insight for oxygen or nitric oxide reduction
Coorientador
 Doutoramento (Doutoramento)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2005 - 2010/03 Structure to Function Studies in UDP-glucose Dehydrogenase s snd Nitroreductases
Orientador
 Doutoramento (Doutoramento)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2003 - 2007/09/10 Structure to function studies in hybrid cluster proteins and glucose-1-phosphate urydylyltranferases
Orientador
 Douturamento (Doutoramento)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1992 - 1996 Cristalização e Análise Estrutural por Difração de Raios-Xde Metaloproteinas Bacterianas
Coorientador
 Douturamento (Doutoramento)
Instituto Superior Técnico, Universidade Técnica de Lisboa, Portugal
Distinções

Prémio

2008 Prémio Câmara Pestana for Best Study in Microbiology
Instituto Câmara Pestana e GlaxoSmithkline, Portugal

Outra distinção

1996 Postdoctoral fellow poster award