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The PI Carlos Frazão leads the ITQB-NOV Structural Biology Laboratory aiming the understanding of biochemical processes at atomic level, mainly based on the determination of biological macromolecules 3D structures using X-rays diffraction analysis. The interpretation of these structures originates clues on how biomolecules may interact, and has enabled the rationalization of applications in diverse fields of life sciences. The PI promotes the collaborations with other research groups to study key biological macromolecules, like proteins and complexes between proteins and nucleic-acids. The scope of developed projects includes electrons transfer meatllo proteins like iron sulfur proteins, mono and multi-heme cytochromes, hybrid cluster and Rieske proteins, flavo di-iron proteins, heme catalese, sulfur oxygenase-reductase, persulfide dioxigenase as well as various enzymes like the messenger RNA control ribonuclease II, aspartic or cysteine proteases, amidase, manganese superoxide dismutase, poly-saccharide synthesis enzymes, osteocalcine or directed evolution improved enzymes, among other examples.
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Identification

Personal identification

Full name
Carlos Maria de Noronha Galvão Franco Frazão
Date of birth
1959/04/04
Gender
Male

Citation names

  • Frazão, Carlos
  • Carlos Frazao

Author identifiers

Ciência ID
7B13-8949-DA58
ORCID iD
0000-0002-7725-1671

Email addresses

  • frazao@itqb.unl.pt (Professional)

Telephones

Telephone
  • (351) 21 4469666 (Professional)

Addresses

  • Av. da República, 2780-157, Oeiras, Oeiras, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Other Natural Sciences
  • Natural sciences - Other Natural Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Proficiency (C2) Proficiency (C2) Advanced (C1) Proficiency (C2) Advanced (C1)
French Upper intermediate (B2) Advanced (C1) Intermediate (B1) Advanced (C1) Intermediate (B1)
German Intermediate (B1) Intermediate (B1) Upper intermediate (B2) Elementary (A2)
Spanish; Castilian Upper intermediate (B2) Elementary (A2) Advanced (C1) Elementary (A2)
Education
Degree Classification
1984/10/01 - 1988/07/27
Concluded
 dotoramento (Doktor (PhD))
Major in Química
Die Fakultaet fuer Chemie, Biologie und Geowissenshaften der Technischen Universitaet Muenchen verleiht, Germany
"Synthese und Struktur von Silanen und Phosphanen mit verzweigtkettigen und ungesaettigten Substituenten" (THESIS/DISSERTATION)
 Sehr gut bestanden
1978/10/01 - 1983/12/17
Concluded
 Engenharia Qquímica (Mestrado)
Instituto Superior Técnico, Universidade Técnica de Lisboa, Portugal
 16
1977/01 - 1979/05/01
Concluded
 Técnioco de Laboratório (Outros)
Major in Técnioco de Laboratório
Laboratórios Fidelis S.A.R.L, Portugal
 n. a.
1972/10/01 - 1976/09/10
Concluded
 Alínia F (Ensino secundário)
Liceu Nacional de D. Pedro V, Portugal
 18
Affiliation

Science

Category
Host institution 		Employer
2008/02/01 - Current Principal Investigator (Research) Universidade Nova de Lisboa, Portugal
1995/11/01 - 2008/02/01 Research Assistant (Research) Centro de Tecnologia Química e Biológica, Portugal
Centro de Tecnologia Química e Biológica, Portugal
1992/10/01 - 1995/10/01 Auxiliary Researcher (Research) Universidade Nova de Lisboa, Portugal
1990/10/01 - 1992/10/01 Research Assistant (Research) Centro de Tecnologia Química e Biológica, Portugal
1990/03/01 - 1990/09/01 Visiting Researcher (Research) Birkbeck College, University of London, United Kingdom
Birkbeck College, University of London, United Kingdom
1988/09/01 - 1990/02/01 Postdoc (Research) Birkbeck College, University of London, United Kingdom

Teaching in Higher Education

Category
Host institution 		Employer
1984/03/01 - 1984/07/01 Assistant (University Teacher) Instituto Superior Técnico, Universidade Técnica de Lisboa, Portugal
Projects

Contract

Designation Funders
2021/01/01 - 2023/12/31 Como é a resposta microbiana ao óxido nítrico regulada por fosforilação
PTDC/BIA-BQM/0562/2020
Universidade Nova de Lisboa, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2018/10/12 - 2021/10/11 Evolução Laboratorial e Computacional Iterativa de Enzimas Bacteriana Lignolíticas
PTDC/BII-BBF/29564/2017
Universidade Nova de Lisboa, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2018/10/01 - 2021/09/30 Diversidade e complexidadde das proteínas diférricas microbianas com multidomínios redutoras de oxigénio e óxido nítrico de Clostridiales
PTDC/BIA-BQM/27959/2017
Universidade Nova de Lisboa, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2016/05/01 - 2019/10/31 Análise estrutural e funcional do factor de transcrição Haa1 indispensável na resposta e resistência da levedura a ácido acético
PTDC/BBB-BEP/0385/2014
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/05/01 - 2019/10/31 Proteínas diférricas na resposta microbiana a stress oxidativo e nitrosativo
PTDC/BBB-BQB/3135/2014
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/03/01 - 2019/02/28 BIOCATÁLISE E TECNOLOGIA DE ENZIMAS LENHINOLÍTICAS
PTDC/BBB-EBB/0122/2014
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2014/06/30 hCE-2 - expressão e caracterização em modelos in vitro e in silico
PTDC/EBB-BIO/111530/2009
Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/01/01 - 2013/10/31 Projecto Estrutural sobre a Metalómica de Deinococcus radiodurans
PTDC/BIA-PRO/100365/2008
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010/04/01 - 2013/09/30 BIOMYR: Em direcção à engenharia metabólica da via do beta-mirceno de Pseudomonas sp. M1: abordagens de genómica funcional e bioquímica estrutural
PTDC/EBB-BIO/104980/2008
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal

Universidade do Minho, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/01/01 - 2013/06/30 Explorando a fosfomanose isomerase do tipo II BceAJ como alvo para o desenvolvimento de novos antimicrobianos e para aplicações biotechnológicas
PTDC/EBB-BIO/098352/2008
Universidade de Lisboa Instituto Superior Técnico, Portugal

Instituto de Biotecnologia e Bioengenharia, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2008/01/01 - 2011/03/31 Estudos Bioquímicos e Estruturais em UDP-Glucose Desidrogenases
PTDC/QUI/67925/2006
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2005/07/01 - 2007/06/30 Pesquisa de proteínas hiperstáveis em proteomas de hipertermófilos
POCI/BIO/58465/2004
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2005/06/01 - 2007/06/30 Caracterização estrutural de proteínas membranares da cadeia respiratória de um organismo termoacidofílico
POCI/BIA-PRO/55621/2004
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book
  1. Faro, C.; Ramalho-Santos, M.; Veríssimo, P.; Pissarra, J.; Frazão, C.; Costa, J.; Lin, X.-L.; Tang, J.; Pires, E.. Structural and functional aspects of cardosins. 1998.
Book chapter
  1. Vicente, João B.; Carrondo, Maria Arménia; Teixeira, Miguel; Frazão, Carlos. "Structural Studies on Flavodiiron Proteins". In Globins and Other Nitric Oxide-Reactive Proteins, Part B, 3-19. Elsevier, 2008.
    10.1016/s0076-6879(07)37001-8
  2. Frazão, Carlos; McVey, Colin E.; Carrondo, MA. "Determinação da Estrutura da Ribonuclease II: uma enzima importante no controlo da expressão génica.". In Annualia, edited by Colaço, Jorge. Lisboa, Portugal: Editorial Verbo, 2007.
    Published
  3. Carlos Frazao. "Molecular Recognition and Drug Design: The Structural Basis of Specificity of Human and Mouse Renins Defined by X-Ray Analysis of Peptide Inhibitor Complexes". United Kingdom, 1992.
Conference paper
  1. Borges, Patricia T.; Joana Rocha; Alma Popescu; Dalila Mil-Homens; Isabel Sá-Correia; Arsénio Fialho; Carlos Frazao. "Hydrolysis of the thioester intermediate in UDP-glucose dehydrogenases". Paper presented in XXII Congress and General Assembly, International Union of Crystallography (IUCr), 2011.
Conference poster
  1. Borges, Patricia T.; Diogo Silva; Carlos Frazao; Martins, Lígia. "Thermostable variants ant their impact on activity of a dye-decolorizing peroxidase from Pseudomonas putida". Paper presented in UK-Japan meeting on dynamic and time-resolved crystallography, Leicester, UK, 2023.
  2. Masgrau, Laura; Taborda, André; Frazão, Tomás; Rodrigues, Miguel; Fernandez-luengo, Xavier; Sancho, Ferran; Frazão, Carlos; et al. "Combined experimental and computational study for the characterization of Pseudarthrobacter siccitolerans". Paper presented in EuroCarb21, 2023.
  3. Taborda, André; Frazão, Tomás; Rodrigues, Miguel; Fernandez-luengo, Xavier; Sancho, Ferran; Frazão, Carlos; Ventura, Rita; et al. "Unveiling the structural determinants for substrate recognition and catalysis of bacterial C-glycoside oxidases". Paper presented in BIOTRANS 2023, 2023.
  4. Lorena, Constança; Diogo Silva; Carlos Frazao; Martins, Lígia O.; Borges, Patricia T.. "Thermostabilizing strategies performed by two computational methods in a DyP type peroxidase from Pseudomonas putida". Paper presented in 16th International Symposium on Biocatalysis & Biotransformations (BIOTRANS), 2023.
  5. Borges, Patricia T.; Diogo Silva; Carlos Frazao; Martins, Lígia. "STRUCTURAL DETERMINANTS THAT IMPACT THE STABILITY AND ACTIVITY OF A DYEDECOLORIZING PEROXIDASE FROM PSEUDOMONAS PUTIDA MET94". Paper presented in 9 th meeting of ENURS 2023- Annual meeting of Portugues Synchrotron Users, Leiria, Portugal, 2023.
  6. Lorena, Constança; Diogo silva; Carlos Frazao; Martins, Lígia O.; Borges, Patricia T.. "Structural determinants behind the improved thermostability of a DyP type peroxidase variant obtained by computational methods". Paper presented in International School on Biological Crystallization, 2023.
  7. Taborda, André; Frazão, Tomás; Rodrigues, Miguel; Fernandez-luengo, Xavier; Sancho, Ferran; Frazão, Carlos; Masgrau, Laura; Borges, Patricia; Martins, Lígia. "Structure-Function Relationships in Pseudarthrobacter siccitolerans Bacterial Glycoside 3-Oxidase". Paper presented in Novel Enzymes 2023, 2023.
  8. Borges, Patricia T.; Brissos, Vânia; Carlos Frazao; Martins, Lígia. "Role of a flexible loop in the substrate selectivity of an engineered variant of hyperthermophilic multicopper oxidase McoP". Paper presented in 16th International Symposium on Biocatalysis and Biotransformations (Biotrans 2023), La Rochelle, France, 2023.
  9. Salgueiro, Bruno; Martins, Maria C.; Gragera, Marcos; Folgosa, Filipe; Carazo, José; Teixeira, Miguel; Carlos Frazao; Romão, Célia V.. "Structural analysis of tree distinct classes of Flavodiiron proteins obtain by X-ray Crystallography and Single-Particle Cryo-EM". Paper presented in 2nd National Crystallographic Meeting, 2022.
  10. Lorena, Constança; Silva, Diogo; Frazão, Carlos; Martins, Lígia O.; Borges, Patricia T.. Corresponding author: Lorena, Constança. "Structural analysis of a computationally designed DyP-type peroxidase with improved thermostability". Paper presented in 2nd National Crystallographic Meeting, July 15-16, Lisbon, Portugal, 2022.
  11. Salgueiro, Bruno; Ribeiro, Ricardo; Hernandez, Guillem; Cordeiro, Tiago; Romão, Célia V.; Isabel Sá-Correia; Frazão, Carlos. "Haa1 transcription factor DNA binding domain structural analysis of two homologous proteins". Paper presented in 7th European Crystallographic School, 2022.
  12. Borges, Patricia T.; Silva, Diogo; Carlos Frazao; Martins, Lígia O.. "EVOLUTION OF STRUCTURAL PROPERTIES IN A DYE-DECOLORIZING PEROXIDASE FROM PSEUDOMONAS PUTIDA MET94". Paper presented in Oxizymes Conference, Siena, Italy, 2022., 2022.
  13. Frazão, Tomás; Taborda, André; Martins, Lígia O.; Carlos Frazao; Borges, Patricia T.. Corresponding author: Borges, Patricia T.. "Structural insights into a bacterial pyranose 2-oxidase from Pseudoarthrobacter siccitolerans, a candidate enzyme for health diagnosis biosensors". Paper presented in European Federation of Biotechnology - Virtual Conference, 2021.
  14. Borges, Patricia T.; Carlos Frazao; Ligia O. Martins. "Structural insights into the evolution of a dye-decolorizing peroxidase from Pseudomonas putida". Paper presented in NextGenBiocat, 2021.
  15. Borges, Patricia T.; Vania Brissos; Guillem Hernandez; Laura Masgrau; Maria Fatima Lucas; Emanuele Monza; Carlos Frazao; Tiago N. Cordeiro; Lígia O. Martins. "Structural analysis of a laboratory evolution variant of hyperthermophilic Multicopper Oxidase McoA". Paper presented in Protein Engineering Congress USA, 2020.
  16. Vania Brissos; Borges, Patricia T.; Laura Masgrau; Maria Fatima Lucas; Emanuele Monza; Carlos Frazao; Tiago N. Cordeiro; Ligia O. Martins. "Protein Engineering, Structural and Computational Studies to Assess Substrate Specificity Determinants of Multicopper Oxidases". Paper presented in Protein Engineering Congress USA, 2020.
  17. Bruno Salgueiro; Folgosa, Filipe; Romao, Celia V; Hernandez, Guillem; Tiago Cordeiro; Frazão, Carlos; Teixeira, Miguel. "Biochemical and structural characterization of a new Flavorubredoxin from Photobacterium swingsii". Paper presented in BioMetals 2020, 2020.
  18. Martins, Maria C.; Salgueiro, Bruno; Folgosa, Filipe; Romão, Célia V.; Frazão, Carlos; Teixeira, Miguel. "Survival strategies of Clostridium difficile to fluctuating concentrations of oxygen". Paper presented in 1st TIMB3 (Twin to illuminate metals in biology and biocatalysis through biospectroscopy) Annual Meeting, 2019.
  19. Gras, Guillem; Hugo Monteiro; Cordeiro, Tiago; Frazão, Carlos; Salgueiro, Bruno. "Dissecting the Structure of the N-Terminal Cytosolic Tail of the Translocated Intimin Receptor". Paper presented in PT NMR Satellite Meeting to SMASH, 2019.
    10.1063/1.58449
  20. Salgueiro, Bruno; Martins, Maria C.; Folgosa, Filipe; Romão, Célia; Teixeira, Miguel; Frazão, Carlos. "Crystal structures determination of classes A and F flavodiiron proteins from Clostridium difficile". Paper presented in 8th Meeting of Portuguese Synchrotron Radiation Users (ENURS 2019), 2019.
  21. Salgueiro, Bruno; Ribeiro, R.; Romão, C.V.; Hernandez, Guillem; Cordeiro, TN; Sá-Correia, I; Frazão, Carlos. "Haa1 transcription fator DNA binding domain structural analysis of two homologous proteins". Paper presented in I National Crystallography Meeting, 2019.
  22. Monteiro, Hugo; Vieira, Marta; Hernandez, Guillem; González, Amaia; Borges, Patrícia; Frazão, Carlos; Monza, Emanuele; et al. "Dynamic Strucutral Studies ¿ From Pathogenic Disordered Proteins To Folded Relevant Enzymes". Paper presented in Twin to Illuminate Metals in Biology and Biocatalysis through Biospectroscopy (TIMB3), 2018.
  23. Vila Santa, A; Moita, MR; Frazão, Carlos; Henriques, Sílvia F.; Sá-Correia, Isabel; Jarboe, LR; Vinga, Susana; Mira, NP. "Exploring a molecular systems biology approach to setup Saccharomyces cerevisiae as a cell factory for the production of itaconic acid". Paper presented in 32nd International Specialized Symposium in Yeasts, Perugia, 2015.
  24. Bandeiras, T; Freitas, MC; Petrasch, D; Botelho, Hugo M; Gomes, Cláudio M; Kletzin, A; Frazão, Carlos. "Purification, crystallization and preliminary crystallographic analysis of Acidianus ambivalens Rieske ferredoxin 2 (RFd2), a new type of Rieske ferredoxins with an additional disulfide". Paper presented in 1° Encontro Nacional de Utilizadores de Radiação de Sincrotrão, 2012.
  25. Bandeiras, T; Freitas, MC; Botelho, Hugo M; Gomes, Cláudio M; Carrondo, MA; Kletzin, A; Frazão, Carlos. "Crystallographic structure of Acidianus ambivalens Rieske ferredoxin 2 ¿ The disulfide bridge structural role". Paper presented in EMBO Practical Course: High-throughput methods for Protein Production and Crystallization, 2011.
  26. McVey, Colin E.; Barbas, Ana; Amblar, M.; Frazão, Carlos; Arraiano, C.M.; Carrondo, MA. "Crystallisation of and crystallographic analysis of RNase II from E. coli.". Paper presented in Protein Society Meeting, Barcelona, 2005.
Dictionary entry
  1. Vicente, João B; Carrondo, Maria Arménia; Teixeira, Miguel; Frazão, Carlos. "Flavodiiron Proteins: Nitric Oxide and/or Oxygen Reductases". In Encyclopedia of Inorganic and Bioinorganic Chemistry. John Wiley & Sons, Ltd, 2011. http://dx.doi.org/10.1002/9781119951438.eibc0645.
    10.1002/9781119951438.eibc0645
Journal article
  1. Brissos, Vânia; Borges, Patrícia T.; Sancho, Ferran; Lucas, Maria Fátima; Frazão, Carlos; Conzuelo, Felipe; Martins, Lígia O.. "Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases". JBIC Journal of Biological Inorganic Chemistry (2024): http://dx.doi.org/10.1007/s00775-023-02040-y.
    10.1007/s00775-023-02040-y
  2. Frade, Kelly; Silveira, Célia M.; Salgueiro, Bruno A.; Mendes, Sónia; Martins, Lígia O.; Frazão, Carlos; Todorovic, Smilja; Moe, Elin. "Biochemical, Biophysical, and Structural Analysis of an Unusual DyP from the Extremophile Deinococcus radiodurans". Molecules 29 2 (2024): 358. http://dx.doi.org/10.3390/molecules29020358.
    10.3390/molecules29020358
  3. Taborda, André; Frazão, Tomás; Rodrigues, Miguel V.; Fernández-Luengo, Xavier; Sancho, Ferran; Lucas, Maria Fátima; Carlos Frazao; et al. "Mechanistic insights into glycoside 3-oxidases involved in C-glycoside metabolism in soil microorganisms". Nature Communications 14 1 (2023): http://dx.doi.org/10.1038/s41467-023-42000-3.
    10.1038/s41467-023-42000-3
  4. Brissos, Vânia; Borges, Patricia T.; Ferran Sancho; Maria Fátima Lucas; Carlos Frazao; Felipe Conzuelo; Martins, Lígia. "Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases". Journal of Biological Inorganic Chemistry. Accepted (2023):
    Accepted
  5. Brissos, Vânia; Borges, Patrícia T.; Núñez-Franco, Reyes; Lucas, Maria Fátima; Carlos Frazao; Monza, Emanuele; Masgrau, Laura; Cordeiro, Tiago N.; Martins, Lígia O.. "Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase". ACS Catalysis 12 9 (2022): 5022-5035. http://dx.doi.org/10.1021/acscatal.2c00336.
    10.1021/acscatal.2c00336
  6. Borges, Patrícia T.; Silva, Diogo; Silva, Tomás F.D.; Brissos, Vânia; Cañellas, Marina; Lucas, Maria Fátima; Masgrau, Laura; et al. "Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase". Computational and Structural Biotechnology Journal 20 (2022): 3899-3910. http://dx.doi.org/10.1016/j.csbj.2022.07.032.
    10.1016/j.csbj.2022.07.032
  7. Rodrigues, Carolina F.; Borges, Patrícia T.; Scocozza, Magali F.; Silva, Diogo; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia O.. "Loops around the Heme Pocket Have a Critical Role in the Function and Stability of BsDyP from Bacillus subtilis". International Journal of Molecular Sciences 22 19 (2021): 10862. http://dx.doi.org/10.3390/ijms221910862.
    10.3390/ijms221910862
  8. Borges, Patrícia T.; Brissos, Vânia; Hernandez, Guillem; Masgrau, Laura; Lucas, Maria Fátima; Monza, Emanuele; Frazão, Carlos; Cordeiro, Tiago N.; Martins, Lígia O.. "Methionine-Rich Loop of Multicopper Oxidase McoA Follows Open-to-Close Transitions with a Role in Enzyme Catalysis". ACS Catalysis 10 13 (2020): 7162-7176. http://dx.doi.org/10.1021/acscatal.0c01623.
    Published • 10.1021/acscatal.0c01623
  9. Martins, Maria C.; Romão, Célia V.; Folgosa, Filipe; Borges, Patrícia T.; Frazão, Carlos; Teixeira, Miguel. "How superoxide reductases and flavodiiron proteins combat oxidative stress in anaerobes". Free Radical Biology and Medicine 140 (2019): 36-60. http://dx.doi.org/10.1016/j.freeradbiomed.2019.01.051.
    Published • 10.1016/j.freeradbiomed.2019.01.051
  10. Borges, Patrícia T.; Romão, Célia V.; Saraiva, Lígia M.; Gonçalves, Vera L.; Carrondo, Maria A.; Teixeira, Miguel; Frazão, Carlos. "Analysis of a new flavodiiron core structural arrangement in Flv1-¿FlR protein from Synechocystis sp. PCC6803". Journal of Structural Biology 205 1 (2019): 91-102. http://dx.doi.org/10.1016/j.jsb.2018.11.004.
    Published • 10.1016/j.jsb.2018.11.004
  11. Frade, Kelly Stefany Tuna; Fernandes, Andreia Cecília Pimenta; Silveira, Celia Marisa; Frazão, Carlos; Moe, Elin. "A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans: cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis". Acta Crystallographica Section F Structural Biology Communications 74 7 (2018): 419-424. http://dx.doi.org/10.1107/s2053230x18008488.
    10.1107/s2053230x18008488
  12. Frade, Kelly; Fernandes, Andreia; Silveira, Celia; Frazão, Carlos; Todorovic, Smilja; Moe, Elin. "Biophysical and crystallization analysis of a novel peroxidase from Deinococcus radiodurans". Free Radical Biology and Medicine 120 (2018): S115. http://dx.doi.org/10.1016/j.freeradbiomed.2018.04.379.
  13. Pereira, L.; Saraiva, I.H.; Oliveira, A.S.F.; Soares, C.M.; Louro, R.O.; Frazão, C.. "Molecular structure of FoxE, the putative iron oxidase of Rhodobacter ferrooxidans SW2". Biochimica et Biophysica Acta - Bioenergetics 1858 10 (2017): 847-853. http://www.scopus.com/inward/record.url?eid=2-s2.0-85027514977&partnerID=MN8TOARS.
    10.1016/j.bbabio.2017.07.002
  14. Romão, C.V.; Vicente, J.B.; Borges, P.T.; Frazão, C.; Teixeira, M.. "The dual function of flavodiiron proteins: Oxygen and/or nitric oxide reductases". Journal of Biological Inorganic Chemistry 21 1 (2016): 39-52. http://www.scopus.com/inward/record.url?eid=2-s2.0-84959364764&partnerID=MN8TOARS.
    10.1007/s00775-015-1329-4
  15. Romão, C.V.; Vicente, J.B.; Borges, P.T.; Victor, B.L.; Lamosa, P.; Silva, E.; Pereira, L.; et al. "Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase". Journal of Molecular Biology 428 23 (2016): 4686-4707. http://www.scopus.com/inward/record.url?eid=2-s2.0-84996538192&partnerID=MN8TOARS.
    10.1016/j.jmb.2016.10.008
  16. Gonc¸alves, V.L.; Vicente, J.B.; Pinto, L.; Romão, C.V.; Frazão, C.; Sarti, P.; Giuffrè, A.; Teixeira, M.. "Flavodiiron oxygen reductase from Entamoeba histolytica: Modulation of substrate preference by tyrosine 271 and lysine 53". Journal of Biological Chemistry 289 41 (2014): 28260-28270. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907638177&partnerID=MN8TOARS.
    10.1074/jbc.M114.579086
  17. Borges, P.T.; Frazão, C.; Miranda, C.S.; Carrondo, M.A.; Romão, C.V.. "Structure of the monofunctional heme catalase DR1998 from Deinococcus radiodurans". The FEBS journal 281 18 (2014): 4138-4150. http://www.scopus.com/inward/record.url?eid=2-s2.0-84909944927&partnerID=MN8TOARS.
    10.1111/febs.12895
  18. Marques, A.T.; Santos, S.P.; Rosa, M.G.; Rodrigues, M.A.A.; Abreu, I.A.; Frazão, C.; Romão, C.V.. "Expression, purification and crystallization of MnSOD from Arabidopsis thaliana". Acta Crystallographica Section F:Structural Biology Communications 70 5 (2014): 669-672. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905435432&partnerID=MN8TOARS.
    10.1107/S2053230X14007687
  19. Borges, P.T.; Miranda, C.S.; Santos, S.P.; Carita, J.N.; Frazão, C.; Romão, C.V.. "Purification, crystallization and phase determination of the DR1998 haem b catalase from Deinococcus radiodurans". Acta Crystallographica Section F:Structural Biology Communications 70 5 (2014): 659-662. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905447633&partnerID=MN8TOARS.
    10.1107/S2053230X1400764X
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Online resource
  1. Borges, Patricia T.; Brissos, Vânia; Carlos Frazao; Martins, Lígia O.. Crystal structure of a multicopper oxidase 3F3 variant from Pyrobaculum aerophilum. 2024. https://www.rcsb.org/structure/unreleased/8P4G.
  2. Borges, Patricia T.; Frazao, Tomas; Taborda, André; Frazão, Carlos; Martins, Lígia O.. Crystal structure of a bacterial pyranose 2-oxidase in complex with mangiferin. 2023. https://www.rcsb.org/structure/7QVA.
  3. Borges, Patricia T.; Frazao, Tomas; Taborda, André; Frazão, Carlos; Martins, Lígia O.. Crystal structure of a bacterial pyranose 2-oxidase complex with D-glucose. 2023. https://www.rcsb.org/structure/7QFD.
  4. Borges, Patricia T.; Frazao, Tomas; Taborda, André; Carlos Frazao; Martins, Lígia O.. Crystal structure of a bacterial pyranose 2-oxidase from Pseudoarthrobacter siccitolerans. 2023. https://www.rcsb.org/structure/7QF8.
  5. Borges, Patricia T.; Martins, Lígia; Frazão, Carlos. Crystal structure of a DyP-type peroxidase 29E4 variant from Pseudomonas putida. 2022. https://www.rcsb.org/structure/7QZA.
  6. Borges, Patricia T.; Silva, D.; Martins, Lígia; Carlos Frazao. Crystal structure of a DyP-type peroxidase from Pseudomonas putida. 2022. https://www.rcsb.org/structure/7QYQ.
  7. Borges, Patricia T.; Silva, D.; Martins, Lígia; Frazão, Carlos. Crystal structure of a DyP-type peroxidase 6E10 variant from Pseudomonas putida. 2022. https://www.rcsb.org/structure/7QYZ.
  8. Borges, Patricia T.; Rodrigues, C..; Silva, D.,; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia. Crystal structure of a DyP-type peroxidase 5G5 variant from Bacillus subtilis. 2021. https://www.rcsb.org/structure/7PL0.
  9. Borges, Patricia T.; Rodrigues, C..; Silva, D..; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia. Crystal structure of a DyP-type peroxidase from Bacillus subtilis in P3121 space group. 2021. https://www.rcsb.org/structure/7PKX.
  10. Borges, Patricia T.; Brissos, Vânia; Cordeiro, Tiago N.; Martins, Lígia; Carlos Frazao. Crystal structure of McoA multicopper oxidase 2F4 variant from the hyperthermophile Aquifex aeolicus. 2021. https://www.rcsb.org/structure/6TTD.
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2017/02 - Current Licenciatura em Bioquímica
Supervisor
 Licenciatura (Degree)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018/07 - 2019/10 Biocatalysis and Technology of Ligninolytic Enzymes (PTDC/BBB-EBB/0122/2014)
Supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2012 - 2019/05/17 Unraveling structural features of flavodiiron proteins. A detailed structural insight for oxygen or nitric oxide reduction
Co-supervisor
 Doutoramento (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2005 - 2010/03 Structure to Function Studies in UDP-glucose Dehydrogenase s snd Nitroreductases
Supervisor
 Doutoramento (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2003 - 2007/09/10 Structure to function studies in hybrid cluster proteins and glucose-1-phosphate urydylyltranferases
Supervisor
 Douturamento (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1992 - 1996 Cristalização e Análise Estrutural por Difração de Raios-Xde Metaloproteinas Bacterianas
Co-supervisor
 Douturamento (PhD)
Instituto Superior Técnico, Universidade Técnica de Lisboa, Portugal
Distinctions

Award

2008 Prémio Câmara Pestana for Best Study in Microbiology
Instituto Câmara Pestana e GlaxoSmithkline, Portugal

Other distinction

1996 Postdoctoral fellow poster award