André Alexandre Grangeia Gouveia (111A-CB3F-1CEC)

Curriculum Vitae

André Alexandre Grangeia Gouveia

André Alexandre Grangeia Gouveia, completed both the Master degree in Microbiology and Bachelors degree in Biology at Aveiro University, Biology department. At the moment he is working on its PhD fellowship at ITQB NOVA - Instituto de Tecnologia Química e Biológica António Xavier. His mains scientific interests are microbial ecology of extreme habitats, focusing in bacterial extremophiles. Particularly, he is interested in understanding the cellular function that enables tolerance/resistance of extremophile bacteria and its application has a tool to solve major global challenges. Currently, he is working with a model organism for radiation resistance, Deinococcus radiodurans to unveil the mechanisms of resistance to radiation conditions as well as their ability to cope with heavy-metals. His aim is to use Deinococcus species (Deinococcus radiodurans and Deinococcus indicus) has a tool for heavy-metal detoxification of waste water systems. At the moment has aquired expertise in in the design of experimental protocols for D. radiodurans in (1) Structural characterization, including protein expression, purification, crystallization and structure determination; (2) Microscopy, including scanning electron microscopy, transmission electron microscopy as well as Cryo electron microscopy sample preparation (workshop) and negative staining; (3) Biological assays, including oxidative stress response and ionizing radiation survival assays.

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Identificação

Identificação pessoal

Nome completo: André Alexandre Grangeia Gouveia

Nomes de citação

Gouveia, André G.

Identificadores de autor

Ciência ID: 111A-CB3F-1CEC
ORCID iD: 0000-0003-2410-6099

Telefones

Telemóvel

913175197 (Pessoal)

Moradas

Av. da República, 2780-157 , Oeiras, Oeiras, Portugal (Profissional)

Idiomas

Idioma	Conversação	Leitura	Escrita	Compreensão	Peer-review
Português (Idioma materno)
Inglês	Utilizador proficiente (C1)	Utilizador proficiente (C1)	Utilizador proficiente (C1)	Utilizador proficiente (C1)

Formação

	Grau	Classificação
2021/02/01 - 2025/02/01 Em curso	Biochemstry (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2016/09 - 2018/12/17 Concluído	Microbiology (Mestrado) Universidade de Aveiro Departamento de Biologia, Portugal "Evaluation of the antimicrobial activity from Cerâmica Cave (Portugal) bacteria: The case study of Cupriavidus sp." (TESE/DISSERTAÇÃO)	18
2012/09/10 - 2016/06/15 Concluído	Biology (Licenciatura) Universidade de Aveiro Departamento de Biologia, Portugal "Assessment of the antimicrobial potential of cave microorganisms in drug discovery from Cerâmica Cave" (TESE/DISSERTAÇÃO)	15

Percurso profissional

Ciência

	Categoria Profissional Instituição de acolhimento	Empregador
2019/03/15 - 2021/01/31	Estagiário de Investigação (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Outros

	Categoria Profissional Instituição de acolhimento	Empregador
2021/02/01 - Atual	PhD Student	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Projetos

Bolsa

	Designação	Financiadores
2019/03/15 - 2021/01/31	Metals in the radiation resistance bacterium Deinococcus radiodurans, its role in protection up to future applications PTDC/BIA-BQM/31317/2017 Bolseiro de Mestrado Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluído

Produções

Publicações

Artigo em revista	Rollo, Filipe; Martins, Guilherme D.; Gouveia, André; Ithurbide, Solenne; Servant, Pascale; Romão, Célia V.; Moe, Elin. "Insights into the role of three Endonuclease III enzymes for oxidative stress resistance in the extremely radiation resistant bacterium Deinococcus radiodurans". Frontiers in Microbiology 14 September (2023): 1-11. http://dx.doi.org/10.3389/fmicb.2023.1266785. Acesso aberto • Publicado Gouveia, André; Salgueiro, Bruno A.; Ranmar, Dean O.; Antunes, Wilson D. T.; Kirchweger, Peter; Golani, Ofra; Wolf, Sharon G.; et al. "Unraveling the multifaceted resilience of arsenic resistant bacterium Deinococcus indicus". Frontiers in Microbiology 14 August (2023): 1-16. http://dx.doi.org/10.3389/fmicb.2023.1240798. Acesso aberto • 10.3389/fmicb.2023.1240798
Poster em conferência	Gouveia, André; T. N. Silva, Sara; Domanska, Ausra; Laurinmäk, Pasi; Carrondo, Maria A.; Butcher, Sarah; Wolf, Sharon G.; Elbaum, Michael; Romao, Celia V. "Deinococcus species as a tool for bioremediation of heavy-metals.". Trabalho apresentado em 13th International Congress on Extremophiles., 2022. Martins, Guilherme ; Gouveia, André; T. N. Silva, Sara; Rollo, Filipe; Domanska, Ausra; Butcher, Sarah; Moe, Elin; Romao, Celia V. "Unveiling the role of Dps and EndoIII for DNA protection and repair in Deinococcus radiodurans upon exposure to genotoxic stress". Trabalho apresentado em 13th International Congress on Extremophiles., 2022. Gouveia, André; T. N. Silva, Sara; Domanska, Ausra; Laurinmäk, Pasi; Célia V. Romão. "Molecular details of the dual function of Dps2, a nano-cage protein from Deinococcus radiodurans". Trabalho apresentado em 2º Encontro Nacional de Cristalografia. (Portugal), 2022. Gouveia, André; T. N. Silva, Sara; Salgueiro, Bruno A. ; Carrondo, Maria A.; Wolf, Sharon G.; Elbaum, Michael; Romao, Celia V. "Exploring Deinococcus arsenic resistance as a tool for bioremediation". Trabalho apresentado em 9 th National Meeting of Portuguese Users of Synchrotron Radiation. (Portugal), 2022. Gouveia, André; Sara T. N. Silva; Célia V. Romão. "Structural characterization of Deinococcus radiodurans Dps2 with manganese: Expression, purification and crystallization". Trabalho apresentado em BioMetals 2020 - 12th International BioMetals Web-Symposium, 2020. Gouveia, André; Reboleira, Sofia Ana; Gonçalves, J. M. Fernando; Marques, M. Sérgio. "Caves as a source of new antimicrobial agents: the case study of antibacterial activity from microorganisms inhabiting Cerâmica Cave, Portugal". Trabalho apresentado em 24th International Conference on Subterranean Biology, 2018.
Recurso online	Gouveia, André G.; Matias, P.M.; Romao, Celia V. Arsenate reductase (ArsC2) from Deinococcus indicus. 2023. https://www.rcsb.org/structure/8P6M. https://doi.org/10.2210/pdb8P6M/pdb Gouveia, André G.; Matias, P.M.; Romao, Celia V. Arsenate reductase (ArsC2) from Deinococcus indicus, co-crystallized with arsenate. 2023. https://www.rcsb.org/structure/8P5N. https://doi.org/10.2210/pdb8P5N/pdb
Resumo em conferência	Gouveia, André. "Electron dense granules from Deinococcus radiodurans, a metal storage nanocompartment". Trabalho apresentado em Microscopy at the Frontiers of Science, Online, 2021. Publicado Gouveia, André; Reboleira, Sofia Ana; Gonçalves, J. M. Fernandes; Marques, M. Sérgio. "Caves as a source of new antimicrobial agents: the case study of antibacterial activity from microorganisms inhabiting Cerâmica Cave, Portugal". Trabalho apresentado em 24th International Conference on Subterranean Biology, Aveiro, 2018. Publicado

Atividades

Apresentação oral de trabalho

	Título da apresentação	Nome do evento Anfitrião (Local do evento)
2023/09/27	Unveiling the nature of cellular nano-compartments, electron-dense granules by cryo-STEM.	Microscopy at the Frontiers of Science Conference (Braga, Portugal)
2022/09/18	Bacterial nano-compartments from the radation resistant bacterium, Deinococcus radiodurans, their function in the cellular response against stress.	13th International Congress on Extremophiles. (Loutraki, Grécia)
2022/06/27	Unveiling the role of Dps and EndoIII for DNA protection and repair in Deinococcus radiodurans upon exposure to genotoxic stress.	III International Meeting of the Portuguese Society of Genetics (Évora, Portugal)
2021/09/29	Electron Dense Geanules From Deinococcus Radiodurans, A Metal Storage Nanocompartment.	Virtual Conference Microscopy at the Frontiers of Science (Braga, Portugal)

Participação em evento

	Descrição da atividade Tipo de evento	Nome do evento Instituição / Organização
2020/11/23 - 2020/11/26	A short course of online lectures will provide an overview of the approaches and methods used in electron cryo-tomography. Topics to be introduced include basic TEM/STEM optics and operation, methods of sample preparation, lamella preparation and serial surface imaging using the focused ion beam – scanning electron microscope (FIB-SEM), correlative fluorescence microscopy (CLEM), and 3D reconstruction of tilt series tomograms. The course should serve as a useful background for practical hands-on workshops. Oficina (workshop)	IMpaCT’s Electron Tomography Virtual Workshop Weizmann Institute of Science, Israel
2020/11/02 - 2020/11/05	The goal of this workshop is to introduce the subject of Cryo-EM Single Particle. This workshop will have both lectures and practical sessions. In the lectures, there will be a discussion of theoretical principles, applications, problem solving, and presentation by students. In the practical sessions, the participants will have the opportunity to prepare and optimise samples and to collect data from different samples using automated data collection with the FEI Talos Arctica or Jeol 3000 on a direct electron detector. Oficina (workshop)	IMpaCT’s Single Particle Online Workshop Helsingin yliopisto Helsinki Institute of Life Sciences, Finlândia
2019/10/26 - 2019/10/26	ITQB NOVA's Open Day is organized every two years by the researchers of the institute, with the aim of showing the science developed. It is a unique opportunity to discover the institution from the inside, talk to our scientists, get to know more about a scientific career and discover the science in our lives. Exposição	ITQB NOVA’s Open Day Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/09/24 - 2019/09/27	This course aims to transfer efficient individual skills in all the steps involved in scientific writing, from content development and organisation to publishing in ISI journals and presenting at conferences. Here there is an exclusive opportunity to acquire in key techniques single source that enable the researcer at any level of the career to be independent and efficient in communicating science in the written and spoken formats. Outro	Scientific writing course (ITQB) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/07/08 - 2019/07/12	A week-long course with theoretical and practical sessions covering basic aspects of the technique and oriented towards both beginners and routine NMR users. Course topics include basic theoretical aspects of NMR. Introduction to the most commonly used 1D and 2D homonuclear and heteronuclear techniques. Data acquisition and processing with a "hands-on" approach. Oficina (workshop)	12th CERMAX practical course on basic NMR Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/05/16 - 2019/05/16	A SCANSCI - Equipamentos de laboratório Lda organized a seminar with the tópic "OS LIMITES DA MICROSCOPIA" represented by Hitachi High-Technologies. The topics are benchtop electron microscopy (benchtop SEM), scanning electron microscopy (W-SEM e FE-SEM), transmission electron microscopy (TEM )and atomic force microscopy (AFM). Demonstration of Benchtop SEM Hitachi TM4000Plus with a EDX Bruker Quantax 75. Seminário	Seminário Hitachi "OS LIMITES DA MICROSCOPIA" Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Expedição científica

	Descrição da atividade	Instituição / Organização
2023/01/15 - 2023/01/27	Through the IMpaCT European Project, I had the opportunity to learn and perform Cryo-Scanning transmission electron microscopy (STEM) coupled with Energy-dispersive X-ray spectroscopy (EDX) to unveil the metal composition of Deinococcus radiodurans and Deinococcus Indicus polyphosphate granules. During this period of time I was trained to do sample preparation, acquire data of STEM and STEM-EDS. and data processing and analisis using VELOX software. Additionally I also receive the basic training on IMOD for Tomogram reconstruction.	Weizmann Institute of Science, Israel
2022/01/19 - 2022/01/24	I had the opportunity to travel to the ESRF to learn and perform analysis of the elemental distribution of D. radiodurans cells using the the ID16B line that contains an X-ray nanoprobe on microXRF (micro X-ray fluorescence) mode. We also performed single cell analysis of the elemental distribution upon acute ionizing radiation exposure	ESRF, França

Distinções

Prémio

2023	Cryo-EM Grant Program from Thermo Fisher Scientific – Award for Finalist (8th) Research Proposal: Unveiling the function of PolyP granules in metal homeostasis and stress response mechanisms Cryo-EM Grant Program from Thermo Fisher Scientific, Estados Unidos
2022	Best Poster Prize - Deinococcus species as a tool for bioremediation of heavy-metals. 13th International Congress on Extremophiles, Grécia