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I obtained my BSc (2014) and MSc degree (2016) in Biotechnology, at University of Beira Interior (UBI). During my MSc, I developed a lab platform for biosynthesis and purification of STEAP1 protein, a membrane protein with a potential therapeutic effect in prostate cancer. The work “Biosynthesis and isolation of STEAP1 protein from E. coli lysates” comprised the steps of vector design, amplification, host manipulation, biosynthesis and recovery, allowing me to gather experience in several techniques and procedures such as vector design, host transfection, fermentation process, cell line culture and chromatography. This stage of my scientific and academic career allowed to publish one review article as co-author (IF JCR: 2.912) and to disseminate the work through posters and oral communications at international meetings, namely a poster presentation at the 11th YES Meeting, and oral presentation at the XI Annual CICS-UBI Symposium. Moreover, a research paper is under review. After concluding my MSc degree, I received a research grant from Santander Totta/ICI. This study allowed me to continue the work developed during my master’s thesis and the main goal was to unveil the interactome of the target protein. During this grant I was able to establish a totally new co-immunoprecipitation protocol of the STEAP1 protein from tumor cell lines and establish a lab platform for purification through HPLC. In 2019, I returned to academic research, enrolling in the ICON project (Interdisciplinary Challenges On Neurodegeneration), at the Health Sciences Research Center. My work was focused in unveiling the role of the Ago2 protein in the ischemic stroke and its potential role in a therapy for the disease. In this period, I gathered experience in primary cell culture, in vivo experiments with rodents and protein expression and recovery. This work was presented and as an awarded oral communication at the 20th Congress of NEDVC, from Portuguese Society of Internal Medicine, and at the XV Annual CICS-UBI Symposium. From this research project, another research article is underway. In 2021, I joined the R&D Department of Laboratórios Basi, and my responsibilities within this role included the validation of analytic methods, analysis of intermediate and finished product, stability assays, HPLC validation, development of new qualification procedures, creation of SOP’s and reports. Currently, I am working as Life Science Consultant for Capgemini Engineering, where I have enrolled in international projects with big pharmaceutical companies such as Roche and Novartis. My main responsibilities in those projects comprises the validation of medical devices and software as medical devices, computerized system validation, performance tests, design and implementation of lab functionalities, protocols, and the continuous improvement of facilities, helping team with a life science point of view.
Identification

Personal identification

Full name
João Pedro Vicente Pais

Author identifiers

Ciência ID
941E-48BB-400C
Education
Degree Classification
2014 - 2016
Concluded
Biotecnologia (Mestrado)
Major in Biotecnologia Industrial
Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
"Biosynthesis and isolation of STEAP1 protein from Escherichia coli cells lysates." (THESIS/DISSERTATION)
17
2014
Concluded
Biotecnologia (Licenciatura)
Universidade da Beira Interior Faculdade de Ciências, Portugal
"Scaffold compósito obtido através de electrospinning para aplicação biomédica " (THESIS/DISSERTATION)
13
Affiliation

Others

Category
Host institution
Employer
2021/07/19 - Current Consultor Life Sciences Capgemini Engineering, France
2021/02/01 - 2021/06/30 Analista I&D Laboratórios Basi - Indústria Farmacêutica SA, Portugal
2019/02/01 - 2020/12/31 Investigador Bolseiro (Projeto ICON) Universidade da Beira Interior, Portugal
Universidade da Beira Interior, Portugal
2020/01/27 - 2020/01/31 Tutor BeInLab Universidade da Beira Interior, Portugal
Universidade da Beira Interior, Portugal
2017/10 - 2018/08 Monitor de Aulas Práticas - UC Design de Bioprocessos Universidade da Beira Interior, Portugal
Universidade da Beira Interior, Portugal
2017/10 - 2018/08 Investigador Bolseiro (Bolsa de Incentivo a Doutoramento) Universidade da Beira Interior, Portugal
Universidade da Beira Interior, Portugal
Projects

Contract

Designation Funders
2019/02 - Current ICON - Interdisciplinary Challenges On Neurodegeneration
CENTRO-01-0145-FEDER-000013
Research Fellow
Universidade da Beira Interior, Portugal
Ongoing
Outputs

Publications

Conference poster
  1. Pais, JP; Machado-Pereira, M; Saraiva C; Bernardino L.; Cristóvão AC.; Ferreira R.. "Argonaute-2 role in post-ischemia inflammatory response". Paper presented in III International Congress in Health Sciences Research, 2019.
  2. Pais, JP; Machado-Pereira, M; Bernardino, L; Cristovão, AC. "Therapeutic role of Argonaute-2 in stroke". Paper presented in III International Congress in Health Sciences Research, 2019.
  3. Pais, JP; Barroca-Ferreira, J.; Santos M.; Gonçalves M; Maia CJ; Passarinha LA. "Recombinant expression of STEAP1 in E.coli: Screening of bioprocess parameters, compartmentalization and chaperones stabilization". Paper presented in 11th YES Meeting, 2016.
Journal article
  1. Barroca-Ferreira, J.; Pais, J.P.; Santos, M.M.; Goncalves, A.M.; Gomes, I.M.; Sousa, I.; Rocha, S.M.; Passarinha, L.A.; Maia, C.J.. "Targeting STEAP1 Protein in Human Cancer: Current Trends and Future Challenges". Current Cancer Drug Targets 18 3 (2018): 222-230. http://dx.doi.org/10.2174/1568009617666170427103732.
    10.2174/1568009617666170427103732
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/11/29 Argonauta-2 na resposta inflamatória pós-isquémia 20º Congresso do Núcleo de Estudos da Doença Vascular Cerebral
Sociedade Portuguesa de Medicina Interna (Porto, Portugal)
2019/11/14 Argonaute-2 role in post-ischemia inflammatory response III International Congress in Health Sciences Research
Universidade da Beira Interior (Covilhã, Portugal)
2019/11 Papel da Argonauta-2 na recuperação vascular em isquémia 20º Congresso do Núcleo de Estudos da Doença Vascular e Cerebral
Sociedade Portuguesa de Medicina Interna (Porto, Portugal)
2016 Biosynthesis and isolation of STEAP1 protein from E.coli lysates: a promising therapeutic target for prostate cancer IV Jornadas de Química e Bioquímica
Universidade da Beira Interior (Covilhã, Portugal)

Consulting

Activity description Institution / Organization
2021/12 - Current Consultor Externo Roche: Validação de Software de Planeamento de Recursos Empresariais (ERP). Capgemini Engineering, France
2021/10 - 2021/12 Consultor Externo Novartis: Computer System Validation (CSV); Validação de Software como dispositivo médico (SaMD). Capgemini Engineering, France
2021/09 - 2021/11 Consultor Externo ARALAB: Computer System Validation (CSV) Capgemini Engineering, France
Distinctions

Other distinction

2017 Bolsa de Incentivo a Doutoramento Santander Totta / ICI
Banco Santander Portugal, Portugal

Universidade da Beira Interior Instituto Coordenador da Investigação, Portugal