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Graduated in Biochemistry from the Faculty of Sciences of the University of Porto (FCUP) and the Abel Salazar Institute of Biomedical Sciences (ICBAS) since 2015 (Class A - Top Student in the ECTS Grading scale), having completed his final internship at the Science for Life Laboratory (SciLifeLab) / Stockholm University, Sweden, under the Erasmus+ program. Awarded the Prize Prof. Dr. Fernando Serrão from the Faculty of Sciences of the University of Porto (FCUP, 2015) for his distinction in the area of Organic Chemistry. Master in Biotechnology from the University of Minho (UM) since 2017, having carried out his master's dissertation at the company Biomode SA. In 2018, he was awarded an FCT PhD scholarship (first place in the Agricultural and Food Technologies panel) for the development of aptamers for staphylococcal enterotoxins using nucleic acid mimics (NAMs), at the Faculty of Engineering of the University of Porto (FEUP) in collaboration with the National Institute for Agricultural and Veterinary Research, I. P. (INIAV). During this period, he was a visiting PhD student at the Department of Cell Biology and Molecular Genetics, University of Maryland, USA, where he strengthened his knowledge in aptamer selection approaches with NAMs. He completed his PhD in Chemical and Biological Engineering in 2023. Currently, PhD researcher at National Institute for Agricultural and Veterinary Research, I. P. (INIAV) focusing on bacteriology, food safety and diagnostic and/or therapeutic solutions (special interest in the development of aptamers).
Identification

Personal identification

Full name
Ricardo Bruno da Costa Oliveira

Citation names

  • Oliveira, Ricardo

Author identifiers

Ciência ID
441A-683A-40E8
ORCID iD
0000-0001-9175-4278

Email addresses

  • up201200365@fe.up.pt (Professional)
  • ricardo.oliveira@iniav.pt (Professional)

Knowledge fields

  • Agrarian Sciences - Agrarian and Food Biotechnology - Diagnostics
  • Engineering and Technology - Other Engineering and Technologies - Food and Beverages
  • Natural sciences - Biological Sciences - Microbiology
  • Natural sciences - Biological Sciences - Biochemical Research Methods
  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2018/10 - 2023/10
Concluded
Chemical and Biological Engineering (Doutoramento)
Universidade do Porto Faculdade de Engenharia, Portugal
"Application of nucleic acid mimics (NAM) in the selection of aptamers against staphylococcal enterotoxins" (THESIS/DISSERTATION)
Approved unanimously
2023/05/08 - 2023/06/12
Concluded
Transferable skills for engineering: basic teaching skills (Pós-Graduação)
Universidade do Porto Faculdade de Engenharia, Portugal
18
2016 - 2017
Concluded
Biotechnology (Mestrado)
Universidade do Minho, Portugal
"Design and validation of an alternative molecular method using peptide nucleic acid fluorescence in situ hybridization (PNA FISH) for the detection of Campylobacter spp. in food samples" (THESIS/DISSERTATION)
19
2012/10 - 2015/10
Concluded
Biochemistry (Licenciatura)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"Identification of multi-drug resistant Mycobacterium tuberculosis by padlock probes and lateral flow nucleic acid biosensors" (THESIS/DISSERTATION)
17 (ECTS Grading scale: Class A - Top Student)
Affiliation

Science

Category
Host institution
Employer
2023/12 - Current Postdoc (Research) Instituto Nacional de Investigação Agrária e Veterinária, I. P. , Portugal
Instituto Nacional de Investigação Agrária e Veterinária, I. P. , Portugal
2018/10 - 2023/10 Research Trainee (Research) Instituto Nacional de Investigação Agrária e Veterinária, I. P. , Portugal
Instituto Nacional de Investigação Agrária e Veterinária, I. P. , Portugal
2023/02 - 2023/05 Contracted Researcher (Research) Universidade do Porto Faculdade de Engenharia, Portugal
Universidade do Porto Faculdade de Engenharia, Portugal
2018/10 - 2023/02 Research Trainee (Research) Universidade do Porto Faculdade de Engenharia, Portugal
Universidade do Porto Faculdade de Engenharia, Portugal
2019/05 - 2019/07 Visiting Researcher (Research) Fundação para a Ciência e a Tecnologia, Portugal
University of Maryland at College Park Cell Biology and Molecular Genetics, United States
2018/02 - 2018/09 Contracted Researcher (Research) Universidade do Minho Centro de Engenharia Biológica, Portugal
Universidade do Minho Centro de Engenharia Biológica, Portugal
2016/09 - 2017/11 Research Trainee (Research) Biomode SA, Portugal
International Iberian Nanotechnology Laboratory, Portugal
2015/02 - 2015/07 Research Trainee (Research) Stockholms Universitet, Sweden
Science for Life Laboratory (SciLifeLab), Sweden
2013/11 - 2014/06 Research Trainee (Research) REQUIMTE LAQV Porto, Portugal
Universidade do Porto Faculdade de Ciências, Portugal
Projects

Contract

Designation Funders
2022/01/01 - Current APTAcoli: NAM-aptamers for blocking enterotoxins in pig colibacillosis
PTDC/CVT-CVT/4620/2021
Post-doc Fellow
International Iberian Nanotechnology Laboratory, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal

Universidade de Trás-os-Montes e Alto Douro, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/10 - 2023/07 NAM4toxins - De novo selection of toxin-specific aptamers using nucleic acid mimics
POCI-01-0145-FEDER- 028659
PhD Student Fellow
Instituto Nacional de Investigação Agrária e Veterinária, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2023/02/01 - 2023/05/31 FLUDS - Desenvolvimento de sistemas baseados em espectroscopia de fluorescência para deteção microbiana
NORTE-01-0247-FEDER-046970
Research Fellow
Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018/10 - 2022/10 Locked nucleic acid (LNA) aptamers for the detection of Staphylococcal Enterotoxins
SFRH/BD/138883/2018
Fundação para a Ciência e a Tecnologia
2018/02 - 2018/09 Coded-FISH: Development of a novel colour-coded RING FISH method: an innovative tool for the detection of antibiotic resistant clinical pathogens
PTDC/DTP-PIC/4562/2014
Master Student Fellow
Universidade do Minho Centro de Engenharia Biológica, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2023/09 - Current BeSafeBeeHoney - BEekeeping products valorization and biomonitoring for the SAFEty of BEEs and HONEY
CA22105
Researcher
Instituto Nacional de Investigação Agrária e Veterinária, Portugal
European Cooperation in Science and Technology
Ongoing
2022/09 - Current EURESTOP - European Network for diagnosis and treatment of antibiotic-resistant bacterial infections
CA21145
Researcher
Instituto Nacional de Investigação Agrária e Veterinária, Portugal
European Cooperation in Science and Technology
Ongoing
2022/05 - Current BETTER - Biosecurity Enhanced Through Training Evaluation and Raising Awareness
CA20103
Researcher
Instituto Nacional de Investigação Agrária e Veterinária, Portugal
European Cooperation in Science and Technology
Ongoing
2022/01 - 2023/06 NAMShield - Controlo de Salmonella spp, na indústria pecuária, através de aptâmeros nanoensapsulados
POCI-01-0247-FEDER-069756
Researcher
Instituto Nacional de Investigação Agrária e Veterinária, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2020/05 - 2020/12 NAM4Covid19 - Nucleic acid mimic lateral flow assay (NAM-LFA) for the detection of Covid19
19128_596771122
Researcher
Instituto Nacional de Investigação Agrária e Veterinária, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal

Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Oliveira, Ricardo; Azevedo, Andreia S.; Mendes, Luzia. "Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization". In Fluorescence In-Situ Hybridization (FISH) for Microbial Cells, 69-86. Springer US, 2021.
    Published • 10.1007/978-1-0716-1115-9_5
  2. Oliveira, Ricardo; Almeida, Carina; Azevedo, Nuno Filipe. "Detection of Microorganisms by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid". In Methods in Molecular Biology, 217-230. Springer US, 2020.
    10.1007/978-1-0716-0243-0_13
Conference abstract
  1. Margarida Barros; Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "Selection of DNA aptamers for the detection of foodborne toxins". Paper presented in 36th EFFoST International Conference 2022, Dublin, 2022.
  2. Oliveira, Ricardo; Pinho, Eva. "Prevalência e diversidade de Staphylococcus aureus e enterotoxinas estafilocócicas em leite cru na região norte de Portugal". Paper presented in Microbiologia 2022, 2022.
  3. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "In silico prediction of three-dimensional structure and docking of aptamers containing nucleic acid mimics (NAMs)". Paper presented in 4th Doctoral Congress in Engineering, Porto, 2021.
  4. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina; Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "Easy-to-use pipeline to predict NAM-aptamers 3D structure and docking". Paper presented in World Microbe Forum, 2021.
  5. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "Surveillance study of Staphylococcus aureus in raw milk from Northern Portugal: enterotoxins and resistance genetic profiles". Paper presented in Microbiotec'21, 2021.
  6. Oliveira, Ricardo; Ana Sousa; Pinho, Eva; Azevedo, Nuno Filipe; Jeffrey Destefano; Almeida, Carina. "De novo selection of toxin-specific aptamers using nucleic acid mimic". Paper presented in Microbiotec 19, 2019.
Conference paper
  1. Oliveira, Hugo Alexandre Mendes; Duarte, José; Domingues, Rita; Vieira, Maria; Silva, Sónia Carina; Santos, Sílvio Roberto Branco; Oliveira, Ricardo; et al. "Understanding the complete reservoir of bacteriophage depolymerases against A. baumannii capsules". 2022.
Conference poster
  1. Oliveira, Ricardo; Araújo, Daniela; Beatriz Loureiro; Castro, Joana; Silva, Sónia; Almeida, Carina. "ST8475: a new sequence type identified in methicillin-resistant staphylococci (MRS) in Portugal". Paper presented in Congress of Microbiology and Biotechnology 2023, 2023.
  2. Barros, Maria Margarida; Oliveira, Ricardo; Castro, Joana; Campos, Ana Maria; Almeida, Gonçalo; Araújo, Daniela; Silva, Sónia; Outor-Monteiro, Divanildo; Almeida, Carina. "Detecting Enterotoxigenic Escherichia coli in animal production: method development and validation". Paper presented in Congress of Microbiology and Biotechnology 2023, 2023.
  3. Araújo, Daniela; Castro, Joana; Oliveira, Ricardo; Oliveira, Hugo; Fernandes, Liliana; Carvalho, Isabel; Cho, GS; et al. "Characterization of molecular features and virulence profile of Klebsiella pneumoniae and Klebsiella oxytoca isolates from companion animals in Portugal.". Paper presented in Congress of Microbiology and Biotecnology, 2023.
  4. Castro, Joana; Araújo, Daniela; Oliveira, Hugo; Fernandes, Liliana; Oliveira, Ricardo; Cho, GS; Franz, C; et al. "Genomic and phenotypic profiling of multidrug- resistant Klebsiella pneumoniae and Klebsiella variicola isolated from northern Portuguese hospitals". Paper presented in Congress of Microbiology and Biotecnology, 2023.
  5. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "Selection of nucleic acid mimic (NAM) aptamers: from de novo SELEX to post-SELEX enhancement". Paper presented in 5th Doctoral Congress Engineering (DCE23), 2023.
  6. Barros, Maria Margarida ; Oliveira, Ricardo; Campos, Ana Maria; Castro, Joana; Araújo, Daniela ; Silva, Sónia; Outor-Monteiro, Divanildo; Almeida, Carina. "Aptamers for blocking Enterotoxigenic Escherichia coli". Paper presented in Dare2Change: Inovattion-Driven Agrifood Business, 2023.
  7. Barros, Maria Margarida; Campos, Ana Maria; Oliveira, Ricardo; Castro, Joana; Almeida, Gonçalo; Silva, Sónia; Outor-Monteiro, Divanildo; Almeida, Carina. "Detecting Enterotoxigenic Escherichia coli in animal production: method development and validation". Paper presented in Dare2Change, 2023.
  8. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. Corresponding author: Oliveira, Ricardo. "Development of an aptamer based-biosensor for the detection of food toxins". Paper presented in Dare2Change, 2023.
  9. Nunes, Bárbara; Barata, Ana; Oliveira, Ricardo; Guedes, Hugo; Almeida, Carina; Silva, Gabriela; M. J. Saavedra; Almeida, Gonçalo. "Fecal carriage of Listeria monocytogenes in dairy cows". Paper presented in Under One Health Concept, 2022.
  10. Bárbara Nunes; Ana Rita Barata; Oliveira, Ricardo; Guedes, Hugo; Carina Manuela Fernandes Almeida; da Silva, Gabriela Jorge; Saavedra, Maria José ; Almeida, Gonçalo. Corresponding author: Almeida, Gonçalo. "Fecal carriage of Listeria monocytogenes in dairy". Paper presented in I International Congress on Animal and Veterinary Sciences ¿Under One Health Concept¿, 2022.
  11. Campos, Ana Maria; Oliveira, Ricardo; Vieira, Filipa ; Silva, Regina A.; Silva, Regina ; Almeida, Carina. ""Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli in the diagnosis of swine colibacillosis"". 2022.
  12. Oliveira, Ricardo; Pinho, Eva; Almeida, Gonçalo; Nuno Azevedo; Carina Manuela Fernandes Almeida. Corresponding author: Carina Manuela Fernandes Almeida. "Prevalence and diversity of Staphylococcus aureus and staphylococal enterotoxins in raw milk from the North of Portugal". Paper presented in Microbiology 2022, 2022.
  13. Oliveira, Ricardo; Pinho, Eva; Almeida, Gonçalo; Azevedo, Nuno Filipe; Almeida, Carina. "Characterization of Staphylococcus aureus and staphylococcal enterotoxins in raw milk from northern Portugal". Paper presented in FEMS Conference on Microbiology, 2022.
  14. Bárbara Nunes; Ana Rita Barata; Oliveira, Ricardo; Guedes, Hugo; Carina Manuela Fernandes Almeida; Gabriela Silva; Saavedra, Maria José ; Almeida, Gonçalo. Corresponding author: Almeida, Gonçalo. "Raw milk as a source of Listeria monocytogenes". Paper presented in workshop of the National Reference Laboratories for Listeria monocytogenes, 2022.
  15. Oliveira, Ricardo; Pinho, Eva; DeStefano, Jeffrey; Azevedo, Nuno Filipe; Almeida, Carina. "Aptamers For Detection of Staphylococcal Enterotoxins in Food Samples". Paper presented in Dare2Change, 2021.
  16. Oliveira, Ricardo; Pinho, Eva; Almeida, Gonçalo; Azevedo, Nuno Filipe; Almeida, Carina. "Prevalence of Staphylococcus aureus and staphylococcal enterotoxins in raw milk from Northern Portugal". Paper presented in Dare2Change, 2021.
  17. Ana Rita Barata; Oliveira, Ricardo; Bárbara Nunes; Guedes, Hugo; Almeida, Carina; Saavedra, Maria José ; Silva, Gabriela; Almeida, Gonçalo. "Raw milk as a source of food zoonotic pathogens". Paper presented in Dare2Change, 2021.
  18. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "In silico prediction of the functional 3D conformation of NAM aptamers". Paper presented in 1st Symposium on Oligonucleotide Technologies and Therapeutics, 2021.
  19. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "Prediction of single-stranded nucleic acids structure: from the sequence to the three-dimensional docking of aptamers". Paper presented in World Microbe Forum 2021, 2021.
  20. Vindeirinho, Joao; Pinho, Eva; Oliveira, Ricardo; Azevedo, Nuno Filipe; Almeida, Carina. "SARS-CoV-2 detection: a simple solution for end-point sensing of RT-PCR products". Paper presented in 1st Symposium on Oligonucleotide Technologies and Therapeutics, 2021.
  21. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "De novo SELEX of toxin specific aptamers using nucleic acid mimics (NAMs)". Paper presented in Encontro Ciência 2020, 2020.
  22. Oliveira, Ricardo; Sousa, Ana Luísa; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "Prediction and design of aptamer structures and their interaction with a target molecule". Paper presented in Microbiotec 19 - Congress of Microbiology and Biotechnology 19, Coimbra, Portugal, 2019.
  23. Oliveira, Ricardo; Ferreira-Bravo, Irani A; Sousa, Ana Luísa; Pinho, Eva; Azevedo, Nuno Filipe; DeStefano, Jeffrey; Almeida, Carina. "Application of nucleic acid mimics (NAMs) in the selection of aptamers against staphylococcal enterotoxins". Paper presented in 1º Simpósio INIAV para a Segurança Alimentar - Rumo à Alimentação do Futuro, Vila do Conde, Portugal, 2019.
  24. Ana Sousa; Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe; Almeida, Carina. "Prediction of aptamers´ structures and their interaction with staphylococcal enterotoxin A". Paper presented in 1º Simpósio INIAV para a Segurança Alimentar, 2019.
  25. Oliveira, Ricardo; Azevedo, Andreia S; Fernandes, Ricardo; Azevedo, Nuno Filipe; Almeida, Carina. "Identification of genes associated with beta lactam resistance in clinical isolates of Gram negative bacteria". Paper presented in FEMS 2019 - 8th Congress of European Microbiologists, Glasgow, Scotland, 2019.
  26. Oliveira, Ricardo; Sousa, Ana Luísa; Pinho, Eva; Almeida, Gonçalo; Azevedo, Nuno Filipe; Almeida, Carina. "De novo selection of toxin-specific aptamers using nucleic acid mimics". Paper presented in Doctoral Congress in Engineering 2019 - FEUP, Porto, Portugal, 2019.
  27. Oliveira, Ricardo; Rocha, Rui; Sousa, Mário; Cerqueira, Laura; Azevedo, Nuno Filipe (BE1B-918B-A853); Almeida, Carina. "Rapid identification of Campylobacter spp. using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) in food samples". Paper presented in V AEICBAS Biomedical Congress - Abel Salazar Biomedical Sciences Institute (ICBAS), Porto, Portugal, 2017.
Journal article
  1. Oliveira, Ricardo; Pinho, Eva; Barros, Maria Margarida; Azevedo, Nuno Filipe; Almeida, Carina. "In vitro selection of DNA aptamers against staphylococcal enterotoxin A". Scientific Reports 14 1 (2024): http://dx.doi.org/10.1038/s41598-024-61094-3.
    10.1038/s41598-024-61094-3
  2. Vindeirinho, João M.; Oliveira, Ricardo; Pinho, Eva; Guiomar, Raquel; Azevedo, Nuno F.; Almeida, Carina. "The potential of tailed amplicons for SARS-CoV-2 detection in Nucleic Acid Lateral Flow Assays". PLOS ONE 19 5 (2024): e0301234. http://dx.doi.org/10.1371/journal.pone.0301234.
    10.1371/journal.pone.0301234
  3. Araújo, Daniela; Silva, Ana Rita; Fernandes, Rúben; Serra, Patrícia; Barros, Maria Margarida; Campos, Ana Maria; Oliveira, Ricardo; et al. "Emerging Approaches for Mitigating Biofilm-Formation-Associated Infections in Farm, Wild, and Companion Animals". Pathogens 13 4 (2024): 320. http://dx.doi.org/10.3390/pathogens13040320.
    10.3390/pathogens13040320
  4. Oliveira, Ricardo; Barbosa, Ana; Sousa, Mário; Azevedo, Nuno Filipe (BE1B-918B-A853); Cerqueira, Laura; Almeida, Carina. "Using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) to detect Campylobacter spp. in food samples". LWT (2024): 115922. http://dx.doi.org/10.1016/j.lwt.2024.115922.
    10.1016/j.lwt.2024.115922
  5. Castro, J; Oliveira, Ricardo; Fernandes, L; Carvalho; Oliveira, H; Brinks, E; Cyu-Sung, Cho; et al. "Molecular characterization and virulence profile of Klebsiella pneumoniae and Klebsiella oxytoca isolated from ill cats and dogs in Portugal". Veterinary Microbiology (2024): 110056. http://dx.doi.org/10.1016/j.vetmic.2024.110056.
    10.1016/j.vetmic.2024.110056
  6. Nunes, Bárbara; Barata, Ana Rita; Oliveira, Ricardo; Guedes, Hugo; Almeida, Carina; da Silva, Gabriela Jorge; Nogueira, Teresa; Saavedra, Maria José; Almeida, Gonçalo. "Occurrence and diversity of Listeria monocytogenes in Portuguese dairy farms". The Microbe (2024): 100063. http://dx.doi.org/10.1016/j.microb.2024.100063.
    Published • 10.1016/j.microb.2024.100063
  7. Oliveira, Ricardo; Pinho, Eva; Azevedo, Nuno Filipe (BE1B-918B-A853); Almeida, Carina. "Post-SELEX modifications with locked nucleic acids (LNA) of a SEA-specific DNA aptamer assisted by in silico modelling". Molecular Systems Design & Engineering (2024):
    Under revision
  8. Araújo, D.; Castro, J.; Matos, F.; Oliveira, R.; Ramos, C.; Almeida, C.; Silva, S.. "Exploring the prevalence and antibiotic resistance profile of Klebsiella pneumoniae and Klebsiella oxytoca isolated from clinically ill companion animals from North of Portugal". Research in Veterinary Science 159 (2023): 183-188. http://dx.doi.org/10.1016/j.rvsc.2023.04.009.
    10.1016/j.rvsc.2023.04.009
  9. Barros, Maria Margarida; Castro, Joana; Araújo, Daniela; Campos, Ana Maria; Oliveira, Ricardo; Silva, Sónia; Outor-Monteiro, Divanildo; Almeida, Carina. "Swine Colibacillosis: Global Epidemiologic and Antimicrobial Scenario". Antibiotics 12 4 (2023): 682. http://dx.doi.org/10.3390/antibiotics12040682.
    10.3390/antibiotics12040682
  10. Oliveira, Ana; Dias, Carla; Oliveira, Ricardo; Almeida, Carina; Fuciños, Pablo; Sillankorva, Sanna; Oliveira, Hugo. "Paving the way forward: Escherichia coli bacteriophages in a One Health approach". Critical Reviews in Microbiology (2023): 1-18. http://dx.doi.org/10.1080/1040841x.2022.2161869.
    10.1080/1040841x.2022.2161869
  11. Barata, Ana Rita; Nunes, Bárbara; Oliveira, Ricardo; Guedes, Hugo; Almeida, Carina; Saavedra, Maria José; da Silva, Gabriela Jorge; Almeida, Gonçalo. "Occurrence and seasonality of Campylobacter spp. in Portuguese dairy farms". International Journal of Food Microbiology 383 (2022): 109961. http://dx.doi.org/10.1016/j.ijfoodmicro.2022.109961.
    10.1016/j.ijfoodmicro.2022.109961
  12. Oliveira, Ricardo; Castro, Joana; Silva, Sónia; Oliveira, Hugo; Saavedra, Maria José; Azevedo, Nuno Filipe; Almeida, Carina. "Exploring the Antibiotic Resistance Profile of Clinical Klebsiella pneumoniae Isolates in Portugal". Antibiotics 11 11 (2022): 1613. http://dx.doi.org/10.3390/antibiotics11111613.
    Published • 10.3390/antibiotics11111613
  13. Castro, Joana; Barros, Maria Margarida; Araújo, Daniela; Campos, Ana Maria; Oliveira, Ricardo; Silva, Sónia; Almeida, Carina. "Swine enteric colibacillosis: Current treatment avenues and future directions". Frontiers in Veterinary Science 9 (2022): http://dx.doi.org/10.3389/fvets.2022.981207.
    Published • 10.3389/fvets.2022.981207
  14. Oliveira, Ricardo; Pinho, Eva; Sousa, Ana Luísa; Dias, Óscar; Azevedo, Nuno Filipe; Almeida, Carina. "Modelling aptamers with nucleic acid mimics (NAM): From sequence to three-dimensional docking". PLOS ONE 17 3 (2022): e0264701. http://dx.doi.org/10.1371/journal.pone.0264701.
    Open access • 10.1371/journal.pone.0264701
  15. Oliveira, Ricardo; Pinho, Eva; Almeida, Gonçalo; Azevedo, Nuno Filipe; Almeida, Carina. "Prevalence and diversity of Staphylococcus aureus and staphylococcal enterotoxins in raw milk from northern Portugal". Frontiers in Microbiology (2022): https://www.frontiersin.org/articles/10.3389/fmicb.2022.846653/abstract.
    Open access • In press • 10.3389/fmicb.2022.846653
  16. Oliveira, Ricardo; Pinho, Eva; Sousa, Ana Luísa; DeStefano, Jeffrey J.; Azevedo, Nuno Filipe; Almeida, Carina. "Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches". Trends in Biotechnology (2021): http://dx.doi.org/10.1016/j.tibtech.2021.09.011.
    10.1016/j.tibtech.2021.09.011
  17. Fernandes, Ricardo M; Silva, Hélder; Oliveira, Ricardo; Almeida, Carina; Azevedo, Nuno Filipe; Vieira, Maria J. "Morphological transition of Helicobacter pyloriadapted to water". Future Microbiology 12 13 (2017): 1167-1179. http://dx.doi.org/10.2217/fmb-2016-0174.
    10.2217/fmb-2016-0174
Magazine article
  1. Castro, Joana; Barros, Maria Margarida; Oliveira, Ricardo; Araújo, Daniela; Campos, Ana Maria; Ana Margarida Duarte; FAGULHA, TERESA; et al. "Coinfecção pelo Vírus da Síndrome Respiratória e Reprodutiva dos Suínos (PRRS), Actinobacillus pleuropneumoniae, Pasteurella multocida e Streptococcus suis 2 - Relato de um caso numa exploração familiar de suínos em Portugal", Revista Portuguesa de Ciências Veterinárias 119, 625, 2024
Thesis / Dissertation
  1. "Application of nucleic acid mimics (NAM) in the selection of aptamers against staphylococcal enterotoxins". PhD, Universidade do Porto Faculdade de Engenharia, 2023.
  2. Oliveira, Ricardo. "Design and validation of an alternative molecular method using peptide nucleic acid fluorescence in situ hybridization (PNA FISH) for the detection of Campylobacter spp. in food samples". Master, Universidade do Minho Departamento de Engenharia Biológica, 2017.
  3. Oliveira, Ricardo. "Identification of multi-drug resistant Mycobacterium tuberculosis by padlock probes and lateral flow nucleic acid biosensors". Degree, Universidade do Porto, 2015.

Artistic / Performance

Visual artwork
  1. Barros, Maria Margarida; Castro, Joana; Araújo, Daniela; Oliveira, Ricardo; Silva, Sónia; Almeida, Carina. Impacto da Colibacilose Suína: da Quinta ao Homem.
  2. Rúben Fernandes; Vindeirinho, João M. ; Castro, Joana; Araújo, Daniela; Barros, Maria Margarida; Oliveira, Ricardo; Campos, Ana Maria; Silva, Sónia; Almeida, Carina. Os Vilões Invisíveis.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/11/08 Selection of DNA aptamers for the detection of foodborne toxins 36th EFFoST International Conference 2022
(Dublin, Ireland)
2022/10/18 Prevalência e diversidade de Staphylococcus aureus e enterotoxinas estafilocócicas em leite cru na região norte de Portugal Microbiologia 2022
Sociedade Portuguesa de Microbiologia (Portugal)
2022/01/20 Staphylococcal enterotoxins and mycotoxins in raw milk: a combined risk? SciMonday
National Institute for Agricultural and Veterinary Research (INIAV) (Vairão, Portugal)
2021/11/25 Surveillance study of Staphylococcus aureus in raw milk from Northern Portugal: Enterotoxins and resistance genetic profiles. Microbiotec'21
NOVA University of Lisbon (Lisboa, Portugal)
2021/06/28 In silico prediction of three-dimensional structure and docking of aptamers containing nucleic acid mimics (NAMs) 4th Doctoral Congress in Engineering
Faculty of Engineering, University of Porto (Porto, Portugal)
2019/12 Application of nucleic acid mimics in the selection of aptamers against staphylococcal enterotoxins Microbiotec'19 - Congress of Microbiology and Biotechnology 2019
Faculdade de Ciências da Universidade de Coimbra (Coimbra, Portugal)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2024/01/08 - 2024/01/27 Selection and characterization of aptamers against enterotoxigenic E. coli
Supervisor
Ciências Biomédicas Laboratoriais (Degree)
Instituto Politécnico do Porto Escola Superior de Saúde, Portugal
2021/01 - 2021/07 Aptamers for Staphylococcal enterotoxin A: effects on incorporating nucleic acid mimics
Co-supervisor
Biotecnologia Medicinal (Degree)
Instituto Politécnico do Porto Escola Superior de Saúde, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2023/09/06 - Current COST Academy Leadership Workshop
Workshop
European Cooperation in Science and Technology, Belgium
2019/03/21 - Current Design and Synthesis of Nucleic Acids Mimics
Workshop
Syddansk Universitet, Denmark
2024/04/09 - 2024/04/24 e.Biofilm Training School - Fundamentals of Biofilm Engineering
Workshop
e.Biofilm Training School - Fundamentals of Biofilm Engineering
Universidade do Porto Faculdade de Engenharia, Portugal
2020/08/27 - 2020/08/28 Antimicrobial drugs, drug delivery vectors, bio-barriers and characterization assays.
Workshop
DelNAM’s Workshop 3 - “Drug Delivery meets Bacteria"
Universidade do Porto Faculdade de Engenharia, Portugal
2019/05 - 2019/06 Radiation Protection Training for Use of Radiation-Producing Materials
Workshop
University of Maryland at College Park, United States
2018/11/21 - 2018/11/23 XXIV Encontro Luso-Galego de Química
Meeting
XXIV Encontro Luso-Galego de Química
Universidade do Porto Faculdade de Ciências, Portugal

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2022 Development and optimization of a PCR methodology for the detection of the main virulence factors (i.e., toxins and fimbriae) of enterotoxigenic E. coli (ETEC)
Supervisor
Ana Campos (Other)
Instituto Politécnico do Porto Escola Superior de Saúde, Portugal

Association member

Society Organization name Role
2019 - Current Portuguese Society of Microbiology Member

Mentoring / Tutoring

Topic Student name
2022/04 - 2022/05 Development and optimization of a PCR methodology for the detection of the main virulence factors (i.e., toxins and fimbriae) of enterotoxigenic E. coli (ETEC) Ana Ferraz
2021/09/06 - 2021/09/24 Characterization of resistance/virulence genes by PCR of Staphylococcus aureus isolates obtained from raw milk samples. Ana Campos
2021/01 - 2021/07 Aptamers for Staphylococcal enterotoxin A: effects on incorporating nucleic acid mimics Roberto Miguel Pais Pinto
Distinctions

Award

2023 FEMS Early Career Scientist Meeting Grant
Federation Of European Microbiological Societies, Netherlands
2021 Best Presentation Award - 1st place
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
2017 2nd Best Scientific Poster Award - V AEICBAS Biomedical Congress
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2015 Prize Prof. Doutor Fernando Serrão
Universidade do Porto Faculdade de Ciências, Portugal
2015 Erasmus+ Scholarship
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Other distinction

2018 FCT PhD Studentship - SFRH/BD/138883/2018
Fundação para a Ciência e a Tecnologia, Portugal