???global.info.a_carregar???
Manuela Machado is currently a Researcher at the Centro de Biotecnologia e Química Fina (CBQF). She is also a member of its Safety Committee and the Researcher responsible for the Project Vertical Alimentar, part of the PRR-funded Pacto da Bioeconomia Azul project. In this role, she oversees day-to-day interactions with industrial and academic partners and manages the overall technical and financial execution of the project. Throughout her research career, Manuela has been dedicated to developing agro-food-based solutions for the food and nutraceutical industries. Her strong industrial focus is evident in her participation in four industrial R&D projects and one additional project of a more fundamental nature. This industry collaboration resulted in the creation of a functional canned tuna product featuring EFSA-approved cardiovascular health claims, which is currently available on the market. An analysis of her CV highlights three main research themes: i) Valorization of agro-food byproducts within a circular economy framework, exploring their biological potential. ii) Characterization of extracted compounds' biological potential, focusing on properties such as modulation of cellular metabolism and immune response. ii) Design and formulation of delivery solutions for bioactive compounds. Manuela is the author of 37 peer-reviewed international papers and 7 book chapters, serving as the first author in 47% of these publications (17 papers and 4 book chapters). Additionally, she was the Invited Editor of the Bioactive Lipids book published by Elsevier. As of November 2024, her work has been cited 298 times by 274 papers, giving her an h-index of 9, according to Scopus. Her primary publication areas include Agricultural and Biological Sciences, followed by Biochemistry, Genetics, and Molecular Biology. She has presented her research at several national and international conferences, delivering 9 oral presentations and 24 poster communications. Manuela has tutored four visiting MSc students and supervised several BSc students, including 1 as supervisor and 3 as co-supervisor. In recognition of her outstanding scientific contributions during her PhD research, she received the First Prize in the Mentorado Comendador Arménio Miranda competition by Frulact Academy. More recently, she was awarded CBQF's annual incentive for extraordinary outputs in 2023.
Read full resume ↓
Identification

Personal identification

Full name
Manuela de Fátima Ferraz Machado

Citation names

  • Machado, Manuela

Author identifiers

Ciência ID
411B-FA18-090C
ORCID iD
0000-0002-7705-2293

Email addresses

  • mmachado@ucp.pt (Professional)

Addresses

  • Escola Superior de Biotecnologia, Rua de Diogo Botelho, 1327, 4169-005, Porto, Porto, Portugal (Professional)

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2025/01/06 - 2025/01/10
Concluded
 Training Course in Flow Cytometry: From Basic Principles to Clinical Applications (Outros)
Universidade do Porto Faculdade de Farmácia, Portugal
 17
2019/01 - 2023/04/19
Concluded
 Food Science and Technology and Nutrition (Doutoramento)
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
 19
2020/06 - 2020/06
Concluded
 Training course in Cell Culture (Outros)
Major in Cell Culture
Universidade do Porto Faculdade de Medicina, Portugal
 18
2011/09 - 2013/06
Concluded
 Biotechnology and Food Quality (Mestrado)
Universidade de Trás-os-Montes e Alto Douro, Portugal
 17
2008/10 - 2011/06
Concluded
 Food Science (Licenciatura)
Universidade de Trás-os-Montes e Alto Douro, Portugal
 14
Affiliation

Science

Category
Host institution 		Employer
2023/06 - Current Contracted Researcher (Research) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
Portuguesa Centro de Biotecnologia e Química Fina, Portugal
2017/09 - 2018/12 Researcher (Research) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
Portuguesa Centro de Biotecnologia e Química Fina, Portugal
2017/04 - 2017/08 Researcher (Research) Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
2014/07 - 2015/06 Researcher (Research) Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2025 - Current Invited Assistant Professor (University Teacher) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal

Others

Category
Host institution 		Employer
2022/10 - Current Observer Auditor NP EN ISO/IEC 17025:2018 – CINATE Microbiology CINATE - Laboratório de Análises e Ensaios a Alimentos e Embalagens, Portugal
2020/01 - Current Manager of the Processing of Clinical Analysis Laboratory Portuguesa Centro de Biotecnologia e Química Fina, Portugal
Projects

Grant

Designation Funders
2019 - 2023/04/19 Functional lipid enriched dairy products geared towards obesity management: technological feasibility and nutraceutical potential
SFRH/BD/136701/2018
PhD Student Fellow
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2017/09 - 2018/12 FUNCTIONALTUNA Development of functional canned tuna products
POCI-01-0247-FEDER-003466
Master Student Fellow
Portuguesa Centro de Biotecnologia e Química Fina, Portugal
Concluded
2017/04 - 2017/08 INTERACT Integrative Research in Environment, Agro-Chains and Technology
NORTE-01-0145-FEDER-000017
Master Student Fellow
Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
Concluded
2014/07/01 - 2015/06/30 INNOFOOD (Innovation in the food sector through the valorization of food and agro-food by-products)
NORTE-07-0124-FEDER-0000029
Master Student Fellow
Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
Concluded

Contract

Designation Funders
2023/06 - Current BLUE BIOECONOMY INNOVATION PACT
C644915664-00000026
Researcher
NextGenerationEU
2023/01/01 - 2025/12/31 Nutraceutical biopolymeric-biocatalytic microbot against gut inflammatory disorders
2022.02926.PTDC
Team member
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade Católica Portuguesa, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
Outputs

Publications

Book chapter
  1. Machado, Manuela; Silva, Sara; Costa, Eduardo; Manuela Machado; Sara Silva; Eduardo M Costa. "Uses of gellan gum for nutrient delivery". In Application of Gellan Gum as a Biomedical Polymer, 309-321. Elsevier, 2024.
    Published • 10.1016/b978-0-323-91815-2.00025-9
  2. Machado, Manuela; Freitas, Ana C.; Pintado, Manuela; Gomes, Ana Maria. "Challenges in functional foods development based on bioactive lipids". In Bioactive Lipids, 321-337. Elsevier, 2023.
    10.1016/b978-0-12-824043-4.00011-7
  3. Sousa, Sérgio; Machado, Manuela; Carvalho, Ana P.; Pintado, Manuela; Gomes, Ana Maria; Sérgio Sousa; Manuela Machado; et al. "Alternative sources of bioactive lipids: Challenges and perspectives (microalgae, plant seeds)". In Bioactive Lipids, 297-320. Elsevier, 2023.
    Published • 10.1016/b978-0-12-824043-4.00009-9
  4. Salsinha, Ana Sofia; Machado, Manuela; Rodríguez-Alcalá, Luís Miguel; Gomes, Ana Maria; Pintado, Manuela. "Bioactive lipids: Chemistry, biochemistry, and biological properties". In Bioactive Lipids, 1-35. Elsevier, 2023.
    10.1016/b978-0-12-824043-4.00014-2
  5. Machado, Manuela; Gomes, Ana Maria; Pintado, Manuela; Rodríguez-Alcalá, Luís Miguel. "Lipidomics". In Bioactive Lipids, 37-50. Elsevier, 2023.
    10.1016/b978-0-12-824043-4.00005-1
  6. Machado, Manuela; Gomes, Ana Maria; Pintado, Manuela. "Lipid delivery systems for food applications". In Bioactive Lipids, 339-348. Elsevier, 2023.
    10.1016/b978-0-12-824043-4.00003-8
  7. Costa, Eduardo; Machado, Manuela; Pintado, Manuela; Silva, Sara. "Biological macromolecules as immunomodulators". In Biological Macromolecules, 273-287. Elsevier, 2022.
    10.1016/b978-0-323-85759-8.00012-9
Conference poster
  1. M. L. Gigante; D. P. Baptista; K. K. P. Silva; M. Machado; E. Costa; M. Pintado. "Inflammatory potential of high-protein hybrid yogurt". Paper presented in 3rd International Congress on Bioactive Compounds, 2024.
  2. Rui Magalhães; Manuela Machado; Paula Teixeira. "Comparative analysis of fatty acid profiles in listeria monocytogenes isolates from food processing environments grown at 37 ºC and 10 ºC: exploring potential persistence markers". Paper presented in 2º Simpósio INIAV para a Segurança Alimentar- “Sistemas Alimentares Sustentáveis e Seguro”, Vila do Conde, Portugal, 2024.
  3. Rafaela A. F. Geraldo; Manuela Machado; Sara Silva; Eduardo M. Costa; Elisabete Pinto; Manuela Pintado; Delminda Neves; Marta W. Vasconcelos. "Exploring the health benefits of lentil phenolics: antioxidant and immunomodulatory effects". Paper presented in Metabolism Conference 24, 2024.
  4. V. Martins; M. Machado; E. Costa; A. M. Gomes; F. Poças; M. Pintado; R. M. S. C. Morais; A. M. M. B. Morais. "Alga Fucus sp. extracts to produce edible films". Paper presented in Encontro Ciência 2024, 2024.
  5. V. Martins; M. Coelho; M. Machado; E. Costa; A. M. Gomes; F. Poças; M. Pintado; R. M. S. C. Morais; A. M. M. B. Morais. "Characterization of alga Fucus sp. extracts and residues and their application in producing edible films". Paper presented in 8th Conference of the International Society for Applied Phycology, 2024.
  6. Rafaela A. F. Geraldo; Manuela Machado; Sara Silva; Eduardo M. Costa; Elisabete Pinto; Manuela Pintado; Marta W. Vasconcelos. "Comprehensive analysis and immunomodulatory effects of lentil phenolic extracts". Paper presented in RADIANT CREATOR Workshop, 2024.
  7. Mirna Lúcia Gigante; Débora Parra Baptista; Kívea Kássia de Paiva e Silva; Manuela Machado; Eduardo M. Costa; Manuela Pintado. "Age-related gastrointestinal alterations affect adipolysis induced by dairy and hybrid high-protein yogurts in differentiated 3T3-L1 adipocytes". Paper presented in 8th International Conference on Food Digestion, 2024.
  8. Rafaela A. F. Geraldo; Manuela Machado; Sara Silva; Eduardo M. Costa; Carla S. Santos; Elisabete Pinto; Manuela Pintado; Marta W. Vasconcelos. "Lentil-based muffin in vitro digestion: unraveling nutritional insights". Paper presented in 8th International Conference on Food Digestion, 2024.
  9. Sara A. Cunha; Eduardo Apolinário; Manuela Machado; Tânia Bragança Ribeiro; Sandra Borges; Sara Baptista da Silva; Manuela E. Pintado; Patrícia Batista. "The potential of fucoidan to protect against neurodegenerative diseases (gastrointestinal model)". Paper presented in 8th International Conference on Food Digestion, 2024.
  10. Sara Silva; Isa Silva; Manuela Machado; Eduardo M. Costa; Tania B. Ribeiro; Mª Manuela Pintado. "ATR-FTIR analysis of thermoplastic rubber plates containing food byproduct particles: effect of particle size and production order". Paper presented in Analítica 2024, 2024.
  11. Eduardo M. Costa; Manuela Machado; Sara Silva. "GC-FID as a complementary tool to understand fatty acids’ role in cellular antioxidant activity: a pathway towards understanding iROS production in cells exposed to essential oils for cosmeceutical applications". Paper presented in Analítica 2024, 2024.
  12. Manuela Machado; Eduardo M. Costa; Sara Silva; Manuela Pintado. "ATR-FTIR as a high throughput tool for quick screening of lipolysis in 3T3- L1 adipocytes". Paper presented in Analítica 2024, 2024.
  13. A. S. Sousa; M. M. Pintado; R. D. Matos; C. C. Sousa; M. F. Machado; M. Coelho; P. M. Rodrigues; A. M. Magalhães; E. R. Coscueta. "gBIOT - Nutraceutical biopolymeric-biocatalytic microbot against gut inflammatory disorders". Paper presented in XXIX Porto Cancer Meeting, 2023.
  14. Manuela Machado; Luís Alcalá; Ana Maria Gomes; Manuela Pintado. "Can avocado oil enriched fresh cheese modulate the obesity-related metabolism?". Paper presented in XVI Encontro de Química dos Alimentos, 2022.
  15. Maria Marta Ascensão Teixeira Correia; akritas Isaakidis; Sergio Barros; Marta Isabel Correia Coelho; Manuela Machado; Ana Maria Pereira Gomes; Maria Marta de Ascensão Teixeira Correia; Manuela de Fátima Ferraz Machado. "Organic Portuguese extra virgin olive oil fatty acids and polyphenol profiles". Paper presented in Ciência na Base da ação - Congresso científico da APN, 2022.
  16. Akritas Isaakidis; Sérgio Carvalho Barros; Marta Coelho; Manuela Machado; Nuno Rodrigues; José Pereira; Ana Gomes; Marta Correia. "Comparison between two organic extra virgin olive oils from Northern Portugal". Paper presented in Encontro Ciência '22, 2022.
  17. Luís M. G. Castro; Tânia Ribeiro; Manuela Machado; Elisabete Alexandre; Jorge A. Saraiva; Manuela E. Pintado. "Unraveling the role of dehulling on acorn’s nutritional composition". Paper presented in MICROBIOTEC'21, 2021.
  18. Adma N. F. de Melo; Tiago B. Afonso; Tânia Ribeiro; Manuela Machado; Marta Carvalho; Márcio Carocho; Freni Tavaria; et al. "Antioxidant activity, phenolic profile, cytotoxicity and genotoxicity of plant extracts". Paper presented in MICROBIOTEC'21, 2021.
  19. Machado, Manuela; Sousa, Sérgio; Luis M. Rodriguez-Alcalá; Ana Maria Gomes; Manuela Pintado; Manuela de Fátima Ferraz Machado; Sérgio Daniel da Cruz e Sousa; et al. "Impact of vegetable oils on Lacticaseibacillus rhamnosus and Bifidobacterium animalis subsp. lactis growth". Paper presented in Microbiotec21, 2021.
  20. Helena Araújo-Rodrigues; Machado, Manuela; Carlos Pereira; Manuela Pintado; Helena Maria Araújo Rodrigues; Manuela de Fátima Ferraz Machado; Maria Manuela Estevez Pintado. "Inhibition of fish cooking wastewater oxidation with acorn extract". Paper presented in X FOOD TECHNOLOGY INTERNATIONAL SYMPOSIUM - MURCIA FOOD 2021, 2021.
  21. M Machado; CL Seabra; L Rodríguez-Alcalá; AM Gomes; M Pintado. "A novel strategy to incorporate coconut oil in dairy matrices". Paper presented in 17th Euro Fed Lipid Congress And Expo, 2019.
  22. Manuela Machado; Lígia L. Pimentel; Luís M. Rodriguez-Alcalá; Ana Pisco; Margarida Lopes; Ana M. Gomes; Manuela Pintado. "Impact of sterilization on phytosterols in canned tuna-based products". Paper presented in XIV Encontro de Química dos Alimentos, 2018.
  23. L. M. Rodriguez-Alcalá; L. Pimentel; M. Machado; I. Correia; J. A. Pereira; A. M. Gomes; M. Pintado. "Sterol and fatty acid profile in negrinha and santulhana varietal olive oils from Trás-os-Montes region". Paper presented in International Conference on Mediterranean Diet and Gastronomy, 2018.
  24. M. Machado; L. L. Pimentel; L. M. Rodriguez-Alcalá; A. M. Gomes; Maria Manuela Pintado; Lígia Pimentel. "Enhancement of EPA/DHA content in potentially functional canned tuna". Paper presented in 2nd International Conference on Food Bioactives & Health, 2018.
  25. Machado, Manuela; Nelson Machado; Raul Dominguez-Perles; Ana I.R.N.A. Barros. "Análise imediata de açúcares e acidez titulável em mirtilos através de espectroscopia de Infravermelho". Paper presented in 3º Simpósio Nacional de Fruticultura, 2014.
  26. Machado, Manuela; José Manuel Ameida; Maria Teresa Carvalho; João Lopes; Paula Martins-Lopes; Ana I.R.N.A. Barros. "Aplicação da espectroscopia de infravermelho na análise de clones de azeites da cultivar Cobrançosa em diferentes estados de maturação". Paper presented in XVIII Encontro Luso-Galego de Química, 2012.
  27. Machado, Manuela; Anabela Fernandes-Silva; Fernando Nunes; Ana I.R.N.A. Barros. "L-Phenilalanine ammonia-lyase in olives from cv Cobrançosa grown under diferente irrigation regimes". Paper presented in XXII Encontro da Sociedade Portuguesa de Química/10º Encontro Nacional de Química dos Alimentos, 2011.
Edited book
  1. Manuela Pintado; Machado, Manuela; Ana Maria Gomes; Salsinha, Ana Sofia; Luis M. Rodriguez-Alcalá. Bioactive Lipids. Elsevier. 2023.
    10.1016/c2020-0-01077-7 • Editor
Journal article
  1. Añibarro-Ortega, Mikel; Dias, Maria Inês; Petrovic, Jovana; Núñez, Sonia; Calhelha, Ricardo C.; Costa, Eduardo M.; Machado, Manuela; et al. "Valorization of Solanum melongena L. crop by-products: Phenolic composition and in vitro antioxidant, antidiabetic, anti-inflammatory, cytotoxic, and antimicrobial properties". Process Biochemistry 153 (2025): 315-324. https://doi.org/10.1016/j.procbio.2025.04.002.
    10.1016/j.procbio.2025.04.002
  2. Martins, Valter F. R.; Lopes, Ana I.; Machado, Manuela; Costa, Eduardo M.; Ribeiro, Tânia B.; Poças, Fátima; Pintado, Manuela; Morais, Rui M. S. C.; Morais, Alcina M. M. B.. "Biodegradable Films with Polysaccharides, Proteins, and Bioactive Compounds from Lobosphaera sp.: Antioxidant and Antimicrobial Activities". Foods 14 8 (2025): 1327. https://doi.org/10.3390/foods14081327.
    10.3390/foods14081327
  3. Manuela Machado; Sara Silva; Manuela Pintado; Eduardo M. Costa. "Exploring the effects of sweet potato leaves on skin health - from antimicrobial to immunomodulator". Molecules 30 4 (2025): http://hdl.handle.net/10400.14/48163.
    Open access • Published • 10.3390/molecules30040855
  4. Machado, Manuela; Costa, Eduardo M.; Silva, Sara; Gomes, Ana Maria; Pintado, Manuela. "Exploring the therapeutic potential of avocado oil: Insights into obesity metabolism and immune regulation through in vitro models". Biocatalysis and Agricultural Biotechnology 63 (2025): 103464. https://doi.org/10.1016/j.bcab.2024.103464.
    10.1016/j.bcab.2024.103464
  5. Machado, Manuela; Costa, Eduardo M.; Silva, Sara; Gomes, Ana Maria; Pintado, Manuela. "The Role of Unsaturated Fatty Acid-Rich Dairy Products in Adipocyte Metabolism". Molecules 29 23 (2024): 5502. http://dx.doi.org/10.3390/molecules29235502.
    10.3390/molecules29235502
  6. Machado, Manuela; Costa, Eduardo M.; Silva, Sara; Sousa, Sérgio C.; Gomes, Ana Maria; Pintado, Manuela. "Enhancing Medium-Chain Fatty Acid Delivery Through Bigel Technology". Gels 10 11 (2024): 738. http://dx.doi.org/10.3390/gels10110738.
    10.3390/gels10110738
  7. Manuela Machado; Sara Silva; Eduardo M. Costa. "Byproducts as a sustainable source of cosmetic ingredients". Applied Sciences 14 22 (2024): http://hdl.handle.net/10400.14/47456.
    Open access • Published • 10.3390/app142210241
  8. Sousa, Sérgio; Machado, Manuela; Coscueta, Ezequiel; Ferreira, Andreia S.; Nunes, Cláudia; Coimbra, Manuel A.; Freitas, Ana C.; Carvalho, Ana P.; Gomes, Ana M.. "Defatted Nannochloropsis oculata biomass – Waste or resource?". Algal Research 83 (2024): 103695. http://dx.doi.org/10.1016/j.algal.2024.103695.
    10.1016/j.algal.2024.103695
  9. Martins, Valter F. R.; Coelho, Marta; Machado, Manuela; Costa, Eduardo; Gomes, Ana M.; Poças, Fátima; Sperotto, Raul A.; et al. "Integrated Valorization of Fucus spiralis Alga: Polysaccharides and Bioactives for Edible Films and Residues as Biostimulants". Foods 13 18 (2024): 2938. http://dx.doi.org/10.3390/foods13182938.
    10.3390/foods13182938
  10. Melo, Adma; Afonso, Tiago B.; Pedrosa, Mariana C.; Carvalho, Marta; Rodrigues, Cláudia; Dias, Maria Inês; Ribeiro, Tânia; et al. "Phenolic Profile and Antioxidant and Biological Activities of Plant Extracts Rich in Hydrolyzable Tannins". ACS Agricultural Science & Technology (2024): http://dx.doi.org/10.1021/acsagscitech.4c00271.
    Published • 10.1021/acsagscitech.4c00271
  11. Pires, Carla; Leitão, Matilde; Sapatinha, Maria; Gonçalves, Amparo; Oliveira, Helena; Nunes, Maria Leonor; Teixeira, Bárbara; et al. "Protein Hydrolysates from Salmon Heads and Cape Hake By-Products: Comparing Enzymatic Method with Subcritical Water Extraction on Bioactivity Properties". Foods 13 15 (2024): 2418. http://dx.doi.org/10.3390/foods13152418.
    10.3390/foods13152418
  12. Machado, Adriana Rodrigues; Voss, Glenise Bierhalz; Machado, Manuela; Paiva, Jorge A.P.; Nunes, João; Pintado, Manuela. "Chemical characterization of the cultivar ‘Vinhão’ (Vitis vinifera L.) grape pomace towards its circular valorisation and its health benefits". Measurement: Food (2024): 100175. http://dx.doi.org/10.1016/j.meafoo.2024.100175.
    10.1016/j.meafoo.2024.100175
  13. Araújo-Rodrigues, Helena; Ribeiro, Tânia Bragança; Machado, Manuela; Pereira, Carlos D.; Pintado, Manuela E.. "The Potential of Acorn Extract Treatment on PUFAs Oxidative Stability: A Case Study on Fish Cooking Wastewater". Foods 13 6 (2024): 935. http://dx.doi.org/10.3390/foods13060935.
    10.3390/foods13060935
  14. Vilas-Boas, Ana A.; Goméz-García, Ricardo; Machado, Manuela; Nunes, Catarina; Ribeiro, Sónia; Nunes, João; Oliveira, Ana L. S.; Pintado, Manuela. "Lavandula pedunculata Polyphenol-Rich Extracts Obtained by Conventional, MAE and UAE Methods: Exploring the Bioactive Potential and Safety for Use a Medicine Plant as Food and Nutraceutical Ingredient". Foods 12 24 (2023): 4462. http://dx.doi.org/10.3390/foods12244462.
    10.3390/foods12244462
  15. Vieira, Patrícia; Pinto, Carlos A.; James Goodfellow, Brian; Gomes, Ana M.; Sousa, Sérgio; Machado, Manuela; Delgadillo, Ivonne; Saraiva, Jorge A.. "A chemical study of yoghurt produced under isostatic pressure during storage". Food Chemistry 425 (2023): 136434. http://dx.doi.org/10.1016/j.foodchem.2023.136434.
    10.1016/j.foodchem.2023.136434
  16. Machado, Manuela; Sousa, Sérgio; Rodriguez-Alcalá, Luís M.; Gomes, Ana Maria; Pintado, Manuela. "Anti-obesity potential of a yogurt functionalized with a CLNA-rich pomegranate oil". Food Research International 173 (2023): 113364. http://dx.doi.org/10.1016/j.foodres.2023.113364.
    10.1016/j.foodres.2023.113364
  17. Castro, Luís M. G.; Caço, Ana I.; Pereira, Carla F.; Sousa, Sérgio C.; Brassesco, María E.; Machado, Manuela; Ramos, Óscar L.; et al. "Modification of Acorn Starch Structure and Properties by High Hydrostatic Pressure". Gels 9 9 (2023): 757. http://dx.doi.org/10.3390/gels9090757.
    10.3390/gels9090757
  18. Machado, Manuela; Sousa, Sérgio C.; Rodríguez-Alcalá, Luís M.; Pintado, Manuela; Gomes, Ana Maria. "Functional lipid enriched probiotic cheese: Gastrointestinal stability and potential health benefits". International Dairy Journal 144 (2023): 105700. http://dx.doi.org/10.1016/j.idairyj.2023.105700.
    10.1016/j.idairyj.2023.105700
  19. Machado, Manuela; Silva, Sara; Costa, Eduardo M.. "Are Antimicrobial Peptides a 21st-Century Solution for Atopic Dermatitis?". International Journal of Molecular Sciences 24 17 (2023): 13460. http://dx.doi.org/10.3390/ijms241713460.
    10.3390/ijms241713460
  20. Machado, Manuela; Costa, Eduardo M.; Silva, Sara; Rodriguez-Alcalá, Luís M.; Gomes, Ana Maria; Pintado, Manuela. "Understanding the Anti-Obesity Potential of an Avocado Oil-Rich Cheese through an In Vitro Co-Culture Intestine Cell Model". Molecules 28 15 (2023): 5923. http://dx.doi.org/10.3390/molecules28155923.
    10.3390/molecules28155923
  21. Machado, Manuela; Rodriguez-Alcalá, Luís M.; Pintado, Manuela; Gomes, Ana Maria. "Insights into coconut oil's anti-obesity potential: In vitro modulation of lipidic accumulation, adipolysis, and immune response". European Journal of Lipid Science and Technology (2023): http://dx.doi.org/10.1002/ejlt.202300037.
    10.1002/ejlt.202300037
  22. Silva, Sara; Machado, Manuela; Coelho, Marta; Costa, Eduardo M.; Pintado, Manuela. "Insights into the Antimicrobial Potential of Acorn Extracts (Quercus ilex and Quercus suber)". Applied Sciences 13 11 (2023): 6820. http://dx.doi.org/10.3390/app13116820.
    10.3390/app13116820
  23. Salsinha, Ana Sofia; Cunha, Sara A.; Machado, Manuela; Rodríguez-Alcalá, Luís Miguel; Relvas, João B.; Pintado, Manuela. "Assessment of the bioaccessibility and bioavailability prediction of omega 3 and conjugated fatty acids by in vitro standardized digestion model (INFOGEST) and cell model". Food Bioscience 53 (2023): 102635. http://dx.doi.org/10.1016/j.fbio.2023.102635.
    10.1016/j.fbio.2023.102635
  24. Machado, Manuela; Sousa, Sérgio Cruz; Rodríguez-Alcalá, Luís Miguel; Pintado, Manuela; Gomes, Ana Maria. "Bigels as Delivery Systems of Bioactive Fatty Acids Present in Functional Edible Oils: Coconut, Avocado, and Pomegranate". Gels 9 4 (2023): 349. http://dx.doi.org/10.3390/gels9040349.
    10.3390/gels9040349
  25. Machado, Manuela; Rodríguez-Alcalá, Luís M.; Maria Gomes, Ana; Pintado, Manuela. "Does the Nature of Added Bioactive Lipids Affect the Biological Properties of Yogurts? - Case Study Coconut and Avocado Oils". Applied Sciences 13 5 (2023): 3101. http://dx.doi.org/10.3390/app13053101.
    10.3390/app13053101
  26. Silva, Sara; Costa, Eduardo M.; Machado, Manuela; Morais, Rui M.; Calhau, Conceição; Pintado, Manuela. "Selective Activity of an Anthocyanin-Rich, Purified Blueberry Extract upon Pathogenic and Probiotic Bacteria". Foods 12 4 (2023): 734. http://dx.doi.org/10.3390/foods12040734.
    10.3390/foods12040734
  27. Báo, Sônia Nair; Machado, Manuela; Da Silva, Ana Luisa; Melo, Adma; Cunha, Sara; Sousa, Sérgio S.; Malheiro, Ana Rita; et al. "Potential Biological Properties of Lycopene in a Self-Emulsifying Drug Delivery System". Molecules 28 3 (2023): 1219. http://dx.doi.org/10.3390/molecules28031219.
    10.3390/molecules28031219
  28. Sousa, Sérgio C.; Machado, Manuela; Freitas, Ana C.; Gomes, Ana M.; Carvalho, Ana P.. "Can Growth of Nannochloropsis oculata under Modulated Stress Enhance Its Lipid-Associated Biological Properties?". Marine Drugs 20 12 (2022): 737. http://dx.doi.org/10.3390/md20120737.
    10.3390/md20120737
  29. Costa, Eduardo M.; Silva, Sara; Machado, Manuela; Sousa, Sérgio C.; Tavaria, Freni K.; Pintado, Manuela. "Chitosan Nanoparticles as Bioactive Vehicles for Textile Dyeing: A Proof of Concept". Polymers 14 22 (2022): 4821. http://dx.doi.org/10.3390/polym14224821.
    10.3390/polym14224821
  30. Silva, Sara; Costa, Eduardo M.; Machado, Manuela; Morais, Rui; Calhau, Conceição; Pintado, Manuela. "Antiadhesive and Antibiofilm Effect of Malvidin-3-Glucoside and Malvidin-3-Glucoside/Neochlorogenic Acid Mixtures upon Staphylococcus". Metabolites 12 11 (2022): 1062. http://dx.doi.org/10.3390/metabo12111062.
    10.3390/metabo12111062
  31. Machado, Manuela; Sousa, Sérgio; Morais, Pilar; Miranda, Arménio; Rodriguez-Alcalá, Luís M.; Gomes, Ana Maria; Pintado, Manuela. "Medium-chain triglycerides and conjugated linolenic acids in functional yogurts: impact of GIT and potential biological activities". Food & Function (2022): http://dx.doi.org/10.1039/d2fo01723g.
    10.1039/d2fo01723g
  32. Machado, Manuela; Costa, Eduardo M.; Silva, Sara; Rodriguez-Alcalá, Luís M.; Gomes, Ana M.; Pintado, Manuela. "Pomegranate Oil's Potential as an Anti-Obesity Ingredient". Molecules 27 15 (2022): 4958. http://dx.doi.org/10.3390/molecules27154958.
    10.3390/molecules27154958
  33. Machado, Manuela; Sousa, Sérgio; Morais, Pilar; Miranda, Arménio; Rodriguez-Alcalá, Luís M.; Gomes, Ana Maria; Pintado, Manuela. "Novel avocado oil-functionalized yogurt with anti-obesity potential: Technological and nutraceutical perspectives". Food Bioscience (2022): 101983. http://dx.doi.org/10.1016/j.fbio.2022.101983.
    10.1016/j.fbio.2022.101983
  34. Gómez-García, Ricardo; Vilas-Boas, Ana A.; Machado, Manuela; Campos, Débora A.; Aguilar, Cristóbal N.; Madureira, Ana R.; Pintado, Manuela. "Impact of simulated in vitro gastrointestinal digestion on bioactive compounds, bioactivity and cytotoxicity of melon (Cucumis melo L. inodorus) peel juice powder". Food Bioscience 47 (2022): 101726. http://dx.doi.org/10.1016/j.fbio.2022.101726.
    10.1016/j.fbio.2022.101726
  35. Bonifácio, Teresa; Ana A. Vilas-Boas; Machado, Manuela; Eduardo M. Costa; Sara Silva; Ricardo N. Pereira; Débora A. Campos; Teixeira, José A.; Manuela Pintado. "Exploring the bioactive potential of brewers spent grain ohmic extracts". Innovative Food Science & Emerging Technologies 76 (2022): 102943-102943. http://dx.doi.org/10.1016/j.ifset.2022.102943.
    10.1016/j.ifset.2022.102943
  36. Machado, Manuela; Rodriguez-Alcalá, Luís M.; Gomes, Ana M; Pintado, Manuela. "Vegetable oils oxidation: mechanisms, consequences and protective strategies". Food Reviews International (2022): 1-18. http://dx.doi.org/10.1080/87559129.2022.2026378.
    Published • 10.1080/87559129.2022.2026378
  37. L.M.G. Castro; T.B. Ribeiro; M. Machado; E.M.C. Alexandre; J.A. Saraiva; M. Pintado. "Unraveling the Effect of Dehulling Methods on the Nutritional Composition of Acorn Quercus spp". Journal of Food Composition and Analysis (2021): https://doi.org/10.1016/j.jfca.2021.104354.
    10.1016/j.jfca.2021.104354
  38. Pimentel, Lígia; Fontes, Ana Luiza; Salsinha, Sofia; Machado, Manuela; Correia, Inês; Gomes, Ana Maria; Pintado, Manuela; Rodríguez-Alcalá, Luís Miguel. "Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis". ELECTROPHORESIS 39 15 (2018): 1835-1845. http://dx.doi.org/10.1002/elps.201700425.
    Published • 10.1002/elps.201700425
  39. Machado, Manuela; Machado, Nelson; Gouvinhas, Irene; Domínguez-Perles, Raúl; Barros, Ana I.R.N.A.. "FTIR chemometrical approach for clonal assessment: Selection of Olea europaea L. optimal phenotypes from cv. Cobrançosa". Journal of Chemometrics 31 5 (2017): e2860. http://dx.doi.org/10.1002/cem.2860.
    10.1002/cem.2860
  40. Machado, Manuela; Machado, Nelson; Gouvinhas, Irene; Cunha, Maria; de Almeida, José M. M. M.; Barros, Ana I. R. N. A.. "Quantification of Chemical Characteristics of Olive Fruit and Oil of cv Cobrançosa in Two Ripening Stages Using MIR Spectroscopy and Chemometrics". Food Analytical Methods 8 6 (2014): 1490-1498. http://dx.doi.org/10.1007/s12161-014-0017-2.
    10.1007/s12161-014-0017-2
  41. Machado, Manuela; Felizardo, Carlos; Fernandes-Silva, Anabela A.; Nunes, Fernando M.; Barros, Ana. "Polyphenolic compounds, antioxidant activity and l-phenylalanine ammonia-lyase activity during ripening of olive cv. “Cobrançosa” under different irrigation regimes". Food Research International 51 1 (2013): 412-421. http://dx.doi.org/10.1016/j.foodres.2012.12.056.
    10.1016/j.foodres.2012.12.056
Journal issue
  1. Machado, Manuela; Costa, Eduardo; Ana Maria Gomes. "Gel Technology for Development of Bioactive Foodstuffs". Gels (2024):
  2. Machado, Manuela; Costa, Eduardo; Sara Silva; Sousa, Sérgio C.; Ana Maria Gomes. "Recent Advances in Soft Gels in the Food Industry and Technology". Gels (2024):
Thesis / Dissertation
  1. "Functional lipid enriched dairy products geared towards obesity management: technological feasibility and nutraceutical potential". PhD, Universidade Católica Portuguesa Escola Superior de Biotecnologia, 2023.
  2. Machado, Manuela. "Aplicação da espectroscopia de infravermelho na análise de clones de azeitonas e azeites cv. “Cobrançosa” em dois estados de maturação". Master, Universidade de Trás-os-Montes e Alto Douro, 2013.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/10/11 Unraveling the Gastrointestinal Therapeutic Potential of Flavonoid-Rich Extract Encapsulated in Pectin from Upcycled Orange Peels: Bioaccessibility, Bioactivity, and Gut Microbiota Impact XVII Encontro de Química dos Alimentos: XVII EQA
(Vila Real, Portugal)
2024/10/10 Oleogel Strategy to Reduce Saturated Fats in Vegan Wafer Biscuit Filling Cream XVII Encontro Nacional de Química dos Alimentos
(Vila Real, Portugal)
2024/10/09 Sardine by-products as a sustainable bioactive ingredient XVII Encontro Nacional de Química dos Alimentos
(Vila Real, Portugal)
2024/04/11 Unraveling the full therapeutical potential of polyunsaturated fatty acids: a complete study of omega-3 and conjugated fatty acids bioaccessibility, bioavailability prediction and impact on gut microbiota 8th International Conference on Food Digestion
(Porto, Portugal)
2022/12/06 Bioaccessibility determination of omega-3 and conjugated linolenic acid using an in vitro standardized digestion model (INFOGEST) by GC-FID 12º Encontro Nacional de Cromatografia
Sociedade Portuguesa de Química (Aveiro, Portugal)
2021/09/06 Avocado oil yogurt: development, characterization, and impact of gastrointestinal digestion XV Encontro de Química dos Alimentos
(Funchal, Portugal)
2019/06 Goat Probiotic Whey Cheese: Development and Nutritional Value” presented at XX EuroFoodChem XX EuroFoodChem Conference
(Porto, Portugal)
2014/11/27 “Infravermelho vs métodos colorimétricos: Correlação entre conteúdo fitoquímico e atividade antioxidante em mirtilos XX Encontro Luso-Galego de Química
(porto, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2025/02/03 - 2025/07 Peptide-Based Nanosystems for Non-Alcoholic Fatty Liver Disease Treatment
Supervisor of Ana Carina Santos
 Engenharia Biomédica (Master)
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2024/01/15 - 2024/07/11 Encapsulação de frações peptídicas provenientes de hidrolisados proteicos de Tenébrio molitor: avaliação do seu impacto na resposta inflamatória usando modelos celulares.
Supervisor
 Biotecnologia (Degree)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2024/01/15 - 2024/07/11 Produção de extratos fenólicos de folha de batata doce para aplicação antimicrobiana usando modelos celulares de infeção
Co-supervisor
 Biotecnologia (Degree)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2024/03 - 2024/07 Fenólicos de vinho do Porto como modeladores da resposta de células intestinais na presença de microrganismos patógenicos e comensais
Co-supervisor
 Microbiologia (Degree)
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2024/01/15 - 2024/06/30 Encapsulação de frações peptídicas provenientes de hidrolisados proteicos de Tenébrio molitor: avaliação do seu impacto na resposta inflamatória usando modelos celulares.
Supervisor
Polytechnic Institute of Coimbra, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2024/09/27 - 2024/09/27 Outreaching activity "Biorefinarias: Uma Revolução Industrial Verde"
Exhibition
 Noite Europeia dos Investigadores
i3S - Instituto de Investigação e Inovação em Saúde, Portugal, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2024/11 - Current Food and Chemical Toxicology Elsevier

Committee member

Activity description
Role 		Institution / Organization
2021 - Current Member of Safety Committee in Center of Biotechnology and Fine Chemistry (CBQF)
Member
 Portuguesa Centro de Biotecnologia e Química Fina, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2025 - Current Immunology Microbiologia Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2025 - Current Immunology Ciências da Nutrição Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal

Mentoring / Tutoring

Topic Student name
2023/02 - 2023/05 Olive Oil Chemical Characterization Jane Maghariky
2022/01 - 2022/04 Olive oil lipid profile characterization Akritas Isaakidis and Sérgio Barros
2020/02 - 2021/07 Extraction and characterization of the lipid profile of Pedobacter lusitanus sp. nov. Ruben Peres
Distinctions

Award

2024 CBQF annual incentive for extraordinary outputs during 2023
Portuguesa Centro de Biotecnologia e Química Fina, Portugal
2024 Innovation Award - 2nd place at Expo Fish Portugal
Docapesca Portos e Lotas SA, Portugal
2021 Desenvolvimento de um iogurte com lípidos bioativos para prevenção da obesidade
Frulact - Indústria Agro-Alimentar, S.A., Portugal