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Identification

Personal identification

Full name
Ivan Salazar

Citation names

  • Salazar, IL

Author identifiers

Ciência ID
231A-75CA-AD8C
ORCID iD
0000-0001-9667-6766

Email addresses

  • ivan.salazar@cnc.uc.pt (Professional)

Addresses

  • Faculdade de Medicina da Universidade de Coimbra, Pólo I, 2º Piso, Laboratório Nº 206-A, Rua Larga, 3004-504, Coimbra, Coimbra, Portugal (Professional)

Websites

  • http://www.cnbc.pt/research/department_group_show.asp?iddep=1107&idgrp=1110#34 (Professional)

Knowledge fields

  • Medical and Health Sciences - Basic Medicine - Neurosciences
  • Natural sciences - Biological Sciences - Biochemistry
  • Medical and Health Sciences - Basic Medicine - Neurosciences

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2012/09 - 2017/02
Concluded
Biologia Experimental e Biomedicina (Doutoramento)
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
"Regulation of the Ubiquitin-Proteasome System in brain ischemia: impact on the neuronal proteome" (THESIS/DISSERTATION)
Approved with praise and distinction by unanimity
2010/10 - 2012/07
Concluded
Investigação Biomédica (Mestrado integrado)
Universidade de Coimbra Faculdade de Medicina, Portugal
"Downregulation of the Ubiquitin-Proteasome System under excitotoxic conditions" (THESIS/DISSERTATION)
18
2007/10 - 2010/07
Concluded
Biologia (Licenciatura)
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
"Biology" (THESIS/DISSERTATION)
15
Affiliation

Teaching in Higher Education

Category
Host institution
Employer
2021/09 - 2022/02 Invited Assistant Professor (University Teacher) Universidade de Coimbra Departamento de Ciências da Vida, Portugal

Others

Category
Host institution
Employer
2022/04/01 - Current PostDoctoral Fellow Universidade de Coimbra Multidisciplinary Institute of Ageing, Portugal
2019/02/01 - 2021/12/31 Doctorate Investigator (DL57) within the project: Local protein synthesis and alterations in the synaptic proteome in epileptogenesis (POCI-01-0145-FEDER-028656) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/06/01 - 2019/01/31 PostDoctoral Fellowship (CENTRO-01-0145-FEDER-000008: BrainHealth 2020 - Early Detection, Neuromodulation and Advanced Therapies to Brain Disorders) Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2017/04/01 - 2017/05/31 PostDoctoral Fellowship (Novel cerebrospinal fluid and serum biomarkers for Multiple Sclerosis patients Multiple Sclerosis_RG-1601-075) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2013/02/01 - 2017/02/07 Doctoral Programme in Experimental Biology and Biomedicine (Fellowship SFRH/BD/51967/2012), "Regulation of the Ubiquitin-Proteasome System in the ischemic brain: impact on the neuronal proteome Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2015/05 - 2015/06 Visiting Fellow at the Laboratory Proteolytic Systems, Institut für Biochemie, Charité – Universitätsmedizin Berlin Charité Universitätsmedizin Berlin, Germany
2011/06/01 - 2012/07/30 Graduate student, Master thesis "Dysregulation of the Ubiquitin-Proteasome System in the oxygen and glucose deprivation (OGD) model of transient global ischemia". Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2010/07/10 - 2010/08/10 Short term Summer Fellowship. Universidade de Coimbra, Portugal
Projects

Grant

Designation Funders
2019 - 2021/12 BDNF-induced alterations in the synaptic proteome and neuronal excitability in the pilocarpine model of temporal lobe epilepsy
PTDC/MED-NEU/28656/2017
Researcher
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Associação para a Inovação e Desenvolvimento da FCT
Concluded
2016 - 2020/10 Novel cerebrospinal fluid and serum biomarkers for Multiple Sclerosis
Researcher
National Multiple Sclerosis Society
Ongoing
2017 - 2019/01/31 Detecção Precoce, Neuro-modulação e Terapias Avançadas para Neuropatologias
Post-doc Fellow
Ongoing
2012 - 2017/02 Full PhD Student Fellowship Awarded by the Technology Science Foundation entitled Regulation of the Ubiquitin-Proteasome System in brain ischemia: impact on the neuronal proteome
(SFRH/BD/ 51967/2012)
PhD Student Fellow
Concluded
2012 - 2015/08 Regulation of the ubiquitin-proteasome system by BDNF at the hippocampal synapses: role in synaptic plasticity
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Conference poster
  1. Salazar, IL. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia. Salazar, IL., Frisari., S., Caldeira, MV., Li, KW., Duarte, CB". Paper presented in EMBO Workshop on Proteostasis: from organelles to organisms. Ericeira, Portugal., 2019.
  2. Salazar, IL. "Novel cerebrospinal fluid biomarkers of Multiple Sclerosis. Salazar, IL., Loureço, AS., Baldeiras, I., Teixeira, AC., Grãos, M., Manadas, B., Pais, AC., Novo, AM., Batista, S., Sousa, L., Saraiva, MJ., Duarte, CB". Paper presented in XVI Meeting of the Portuguese Society for Neuroscience. Lisbon, Portugal., 2019.
  3. Salazar, IL. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia. Salazar, IL., Frisari., S., Caldeira, MV., Duarte, CB". Paper presented in 1st Neuroscience and Disease Retreat from the Center of Neuroscience and Cell Biology. Miranda do Corvo, Portugal., 2019.
  4. Salazar, IL. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia. Frisari, S., Salazar, IL., Caldeira, MV., Duarte, CB". Paper presented in XVII SINS National Congress. Ischia, Italy., 2017.
  5. Salazar, IL. "Interplay between the Ubiquitin-Proteasome System and calpains in brain ischemia. Salazar, IL., Caldeira, MV., & Duarte, CB". Paper presented in ISN-ESN biennial meeting. Paris, France., 2017.
  6. Salazar, IL. "Brain ischemia-induced alterations in the ubiquitin-proteasome system: role in neuronal demise. Salazar, IL., Caldeira, MV., & Duarte, CB". Paper presented in 7th ISN Special Neurochemistry Conference- Synaptic Function and Dysfunction In Brain Diseases. Coimbra, Portugal., 2016.
  7. Salazar, IL. "Excitotoxic glutamate stimulation and oxygen and glucose deprivation downregulate the 26S proteasome in cultured neurons. Salazar, IL., Curcio, M., Leal, G., Mele, M., Canzoniero, LM., Duarte, CB. & Caldeira, MV". Paper presented in 9th FENS Forum of Neuroscience . Milan, Italy., 2014.
  8. Salazar, IL. "Glutamate receptor activation is coupled to 26S proteasome downregulation in in vitro ischemia. Salazar, IL., Curcio, M., Leal, G., Mele, M., Canzoniero, LM., Duarte, CB. & Caldeira, MV". Paper presented in 5th Conference on Advances in molecular mechanisms underlying neurological disorders. Bath, UK., 2013.
  9. Salazar, IL. "26S Proteasome downregulation under excitotoxic conditions. Salazar, IL., Caldeira, MV., Curcio, M. & Duarte, CB". Paper presented in X CNC Annual Meeting. Cantanhede, Coimbra., 2012.
Journal article
  1. Diogo Neves; Ivan L. Salazar; Ramiro Almeida; Raquel Silva. "Molecular mechanisms of ischemia and excitotoxicity". Cellular and Molecular Lifesciences (2022):
    Submitted
  2. Salazar, Ivan L.; Lourenço, Ana S. T.; Manadas, Bruno; Baldeiras, Inês; Ferreira, Cláudia; Teixeira, Anabela Claro; Mendes, Vera M.; et al. "Posttranslational modifications of proteins are key features in the identification of CSF biomarkers of multiple sclerosis". Journal of Neuroinflammation 19 1 (2022): http://dx.doi.org/10.1186/s12974-022-02404-2.
    10.1186/s12974-022-02404-2
  3. Mele, Miranda; De Luca, Pasqualino; Santos, Ana Rita; Vieira, Marta; Salazar, Ivan L.; Pinheiro, Paulo S.; Duarte, Carlos B.. "GRASP1 ubiquitination regulates AMPA receptor surface expression and synaptic activity in cultured hippocampal neurons". The FASEB Journal 35 8 (2021): http://dx.doi.org/10.1096/fj.201903061rr.
    10.1096/fj.201903061rr
  4. Singer, W.; Manthey, M.; Panford-Walsh, R.; Matt, L.; Geisler, H.-S.; Passeri, E.; Baj, G.; et al. "BDNF-Live-Exon-Visualization (BLEV) Allows Differential Detection of BDNF Transcripts in vitro and in vivo". Frontiers in Molecular Neuroscience 11 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85054809235&partnerID=MN8TOARS.
    10.3389/fnmol.2018.00325
  5. Salazar, I.L.; Caldeira, M.V.; Curcio, M.; Duarte, C.B.. "The role of proteases in hippocampal synaptic plasticity: Putting together small pieces of a complex puzzle". Neurochemical Research 41 1-2 (2016): 156-182. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946811163&partnerID=MN8TOARS.
    10.1007/s11064-015-1752-5
  6. Curcio, M.; Salazar, I.L.; Mele, M.; Canzoniero, L.M.T.; Duarte, C.B.. "Calpains and neuronal damage in the ischemic brain: The swiss knife in synaptic injury". Progress in Neurobiology 143 (2016): 1-35. http://www.scopus.com/inward/record.url?eid=2-s2.0-84979684030&partnerID=MN8TOARS.
    10.1016/j.pneurobio.2016.06.001
  7. Curcio, M.; Salazar, I.L.; Inácio, A.R.; Duarte, E.P.; Canzoniero, L.M.T.; Duarte, C.B.. "Brain ischemia downregulates the neuroprotective GDNF-Ret signaling by a calpain-dependent mechanism in cultured hippocampal neurons". Cell Death and Disease 6 2 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84927597988&partnerID=MN8TOARS.
    10.1038/cddis.2014.578
  8. Leal, G.; Afonso, P.M.; Salazar, I.L.; Duarte, C.B.. "Regulation of hippocampal synaptic plasticity by BDNF". Brain Research 1621 (2015): 82-101. http://www.scopus.com/inward/record.url?eid=2-s2.0-84941585404&partnerID=MN8TOARS.
    10.1016/j.brainres.2014.10.019
  9. Caldeira, M.V.; Salazar, I.L.; Curcio, M.; Canzoniero, L.M.T.; Duarte, C.B.. "Role of the ubiquitin-proteasome system in brain ischemia: Friend or foe?". Progress in Neurobiology 112 (2014): 50-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84890805926&partnerID=MN8TOARS.
    10.1016/j.pneurobio.2013.10.003
  10. Caldeira, M.V.; Curcio, M.; Leal, G.; Salazar, I.L.; Mele, M.; Santos, A.R.A.; Melo, C.V.; et al. "Excitotoxic stimulation downregulates the ubiquitin-proteasome system through activation of NMDA receptors in cultured hippocampal neurons". Biochimica et Biophysica Acta - Molecular Basis of Disease 1832 1 (2013): 263-274. http://www.scopus.com/inward/record.url?eid=2-s2.0-84868560715&partnerID=MN8TOARS.
    10.1016/j.bbadis.2012.10.009
Journal issue
  1. Salazar, IL. "Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons". BIO-PROTOCOL Vol7 Iss7 (2017): https://bio-protocol.org/e2551.
    Published
Magazine article
  1. Salazar, IL. "Research of new molecular Biomarkers to help diagnosing Multiple Sclerosis", Bulletin of Multiple Sclerosis, 2018, http://spem.pt/wp-content/uploads/2019/08/Boletim-13.pdf.
Thesis / Dissertation
  1. Salazar, Ivan Lalanda. "Regulation of the Ubiquitin-Proteasome System in brain ischemia: impact on the neuronal proteome". PhD, 2017. http://hdl.handle.net/10316/33156.
  2. Salazar, Ivan Lalanda. "Neuronal changes under excitotoxic conditions: the role of 26S proteasome". Master, 2012. http://hdl.handle.net/10316/22782.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/07 Salazar, IL., (2021) Novel Multiple Sclerosis Biomarkers. World Brain Day 2021: a callto stop multiple sclerosis (July, 22nd, Colégio da Trindade, Coimbra, Portugal).
(Portugal)
2021/07 Salazar, IL., Caldeira, MV, Li, KW., & Duarte, CB (2021). Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia. Virtual Symposium on Post-translational modifications in neuronal physiology and brain disorders (5-6 July, National Research Council of Italy).
2017/02/02 The interplay between the Ubiquitin-Proteasome System and calpains in brain ischemia: the effect of proteasome inducer in cell demise Annual meeting of the Portuguese Pharmacological Society
(Coimbra, Portugal)
2013/06/23 Glutamate receptor activation is coupled to 26S proteasome downregulation in in vitro ischemia 5th Conference on Advances in molecular mechanisms underlying neurological disorders
(Bath, United Kingdom)
2013/04/19 26S Proteasome downregulation under excitotoxic conditions 1st Research Coimbra in Health Sciences
(Coimbra, Portugal)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2016/09 - 2017/06 Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia
Supervisor
2013/09 - 2014/06 Redistribution of the Proteasome in Brain Ischemia
Supervisor
2012/01 - 2012/06 Biochemistry Intership - Study of the UPS in brain ischemia
Co-supervisor

Event organisation

Event name
Type of event (Role)
Institution / Organization
2021/05 - 2021/05 Host of Pedro Beltrão, EMBL-EBI, Cambridgeshire. Proteomics approaches to study SARS-CoV-2 biology and to identify antiviral drugs. (2021/05 - 2021/05)
Seminar
2021/03 - 2021/03 Host of Daniel Finley, Department of Cell Biology, Harvard Medical School. Global remodeling during extreme terminal differentiation. (2021/03 - 2021/03)
Seminar
2014/12 - 2014/12 XII Annual CNC meeting “May the Science be with you" (2014/12/15 - 2014/12/16)
Meeting (Co-organisor)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2013/06/08 - 2013/06/08 2nd BEB Symposium – “Seeding curiosity, harvesting Science" (2013/06/08 - 2013/06/08)
Symposium (Co-organisor)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2019/11/15 - 2019/11/19 Workshop on mechanisms of protein quality control
Congress
EMBO Workshop on Proteostasis: from organelles to organisms
2019/05/30 - 2019/06/01 Portuguese Neuroscience Meeting
Congress
XVI Meeting of the Portuguese Society for Neuroscience
2017/08/20 - 2017/08/24 International Neuroscience Meeting
Congress
ISN-ESN biennial meeting
2016/06/01 - 2016/06/04 International Neuroscience Meeting
Congress
7th ISN Special Neurochemistry Conference- Synaptic Function and Dysfunction In Brain Diseases
2014/07/04 - 2014/07/09 International Neuroscience Meeting
Congress
9th FENS Forum of Neuroscience
2013/06/20 - 2013/06/23 International Meeting on Neuronal Proteolysis
Congress
5th Conference on Advances in molecular mechanisms underlying neurological disorders

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2020/09 Unveiling the protective role of Transthyretin in mice stroke model: using a behavioral screening workflow
(Thesis) Main arguer
Raquel Sofia Pinto (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2019/09/15 Unraveling the pathophysiological mechanisms behind white matter lesions: a study on cerebrospinal fluid and blood markers in patients with cerebral small vessel disease
(Thesis) Main arguer
Anuschka da Silva Spínola (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal

Association member

Society Organization name Role
2016 - Current International Society for Neuroschemistry Member
2014/01/09 - Current Biochemical Society Member

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2012/09 - Current Cell Regulation (02001323; Master Program in Cellular and Molecular Biology), laboratory classes for evaluating proteasome activity; aprox 5 hours/year (Mestrado) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2021/09 - 2022/02 Cell Regulation (02001323; Master Program in Cellular and Molecular Biology, FCTUC University of Coimbra), theoretical classes; 7 hours (Cytoskeleton and cell movement: 4.5 hours, Cell Adhesion 1.5 hours, Regulation of gene expression 1 hour) laboratory classes; 44 hours - co-organizer (Mestrado) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2021/09 - 2022/02 Cellular and Molecular Neurobiology (02001298; Master Program in Cellular and Molecular Biology, FCTUC University of Coimbra), TP classes; 16 hours (Journal Club) (Mestrado) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2021/09 - 2022/02 Cellular and Molecular Biology (01000861; Bachelor in Biomedical Engineering/Medicinal Chemistry/Biochemistry, FCTUC University of Coimbra), laboratory classes; 42 hours. (Licenciatura) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2022/01 - 2022/01 Neuroscience (48084; Master Program in Molecular Biomedicine), Ischemia and Reperfusion classes; 2 hours - invited speaker by Ramiro D. Almeida (Mestrado) Universidade de Aveiro Departamento de Ciências Médicas, Portugal
2021/01 - 2021/01 Neuroscience (48084; Master Program in Molecular Biomedicine), Ischemia and Reperfusion classes; 3 hours - invited speaker by Ramiro D. Almeida (Mestrado) Universidade de Aveiro Departamento de Ciências Médicas, Portugal
2020/12 - 2020/12 Neurobiology (14241065; Bachelor in Biomedical Sciences, University of Algarve), Proteostasis in stroke injury Teaching Seminar; 2 hours. (Bacharelato) Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
2019/10 - 2019/10 Neuroscience (48084; Master Program in Molecular Biomedicine), Ischemia and Reperfusion classes; 3 hours - invited speaker by Ramiro D. Almeida (Mestrado) Universidade de Aveiro Departamento de Ciências Médicas, Portugal
2019/03 - 2019/03 Neurobiology and Disease (02001301; Master Program in Cellular and Molecular Biology), Ischemia and Reperfusion classes; 4 hours - invited speaker by Carlos B. Duarte (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2020 - Current Biomolecules (2218-273X) MDPI AG
2020 - Current International Journal of Molecular Sciences (1422-0067) MDPI AG
2020 - Current Cancers (2072-6694) MDPI AG
2020 - Current Cells (Reviewer Member Board) (2073-4409) MDPI AG
2020 - Current International Research Journal of Public and Environmental Health (2360-8803) Journal Issues Limited
2019 - Current Heliyon (Reviewer) (2405-8440) Elsevier
2019 - Current Neurochemical Research (Reviewer) (1573-6903) Springer-Verlag
2019 - Current Journal of Visualized Experiments (Co-Reviewer) (1940-087X) MyJoVE Corporation
2017 - Current Frontiers in Aging Neuroscience (Co-Reviewer) (1663-4365) Frontiers Media SA
2017 - Current Frontiers in Synaptic Neuroscience (Co-Reviewer) (1663-3563) Frontiers Media SA
2017 - Current Scientific Reports (Co-Reviewer) (2045-2322) Springer Science and Business Media LLC
2015 - Current Brain Research (Co-Reviewer) (0006-8993) Elsevier
2015 - Current FEBS Letters (Co-Reviewer) (1873-3468) Wiley (John Wiley & Sons)
2015 - Current Cellular and Molecular Neurobiology (Co-Reviewer) (1573-6830) Springer-Verlag
2015 - Current Journal of Molecular Histology (Co-Reviewer) (1567-2387) Springer-Verlag
2014 - Current Molecular Neurobiology (Co-Reviewer) (0893-7648) Springer-Verlag
2013 - Current PLoS ONE (Co-Reviewer) (1932-6203) Public Library of Science
2012 - Current Neuroscience Letters (Co-Reviewer) (0304-3940) Elsevier
2012 - Current Neurochemical Research (Co-Reviewer) (1573-6903) Springer-Verlag

Mentoring / Tutoring

Topic Student name
2012 - Current Outreaching Activities: Ciência Viva programme,Science&Holliday high-school students,Instituto de Educação e Cidadania IEC holliday students. n.a
Distinctions

Award

2019 Travel grant for the EMBO Workshop Proteostasis: from organelles to organisms, Ericeira, Portugal (15-19 November, 2019) - amount received £450.
Biochemical Society, United Kingdom
2017 Travel grant for the ISN-ENS Biennial meeting, Paris, France (20-24 August, 2017) - amount received £300.
Biochemical Society, United Kingdom
2017 Travel grant and waived registration for the ISN-ENS Biennial meeting, Paris, France (20-24 August, 2017) - amount received 800 USD.
International Society for Neurochemistry, Switzerland
2016 Waived registration for the 7th ISN Special Neurochemistry Conference- Synaptic Function and Dysfunction In Brain Diseases (1-4 June, Coimbra, Portugal).
International Society for Neurochemistry, Switzerland
2013 Travel grant for the “5th Conference on Advances in molecular mechanisms underlying neurological disorders”, Bath, UK
Biochemical Society, United Kingdom
2008 Best 3% alumni in the Biology Bachelor given by the Faculty of Science and Technology
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal

Other distinction

2017 PostDoctoral Fellowship by CNC (Multiple Sclerosis_RG-1601-075 project)
2017 PostDoctoral Fellowship by UC (CENTRO-01-0145-FEDER-000008: BrainHealth 2020 - Early Detection, Neuromodulation and Advanced Therapies to Brain Disorders)
Universidade de Coimbra, Portugal
2012 Full PhD Fellowship in the Experimental Biology and Biomedicine PhD Programme (FCT fellowship SFRH/BD/51967/2012)