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FILIPE JOSÉ MENEZES MERGULHÃO. Completed the PhD in Chemical Engineering in 2002 by Universidade de Lisboa/Instituto Superior Técnico and the degree Chemical Engineering in 1998 (5 year program) by Universidade de Lisboa/Instituto Superior Técnico. Is Assistant Professor in Universidade do Porto/Faculdade de Engenharia and Researcher in Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia. Published 92 articles in international journals and has 11 book chapters. Supervised 5 PhD thesis and co-supervised 2.. Supervised 6 MSc dissertation(s). Has received 1 awards and/or honors. Participates and/or participated as Principal investigator in 7 project(s) and as a Researcher in 23 project(s). Works in the area(s) of Natural sciences with emphasis on Biological Sciences , Engineering and Technology with emphasis on Industrial Biotechnology, Bioproducts, Biomaterials and Medical Biotechnology with emphasis on Biomaterials. His main research activities are in the area of Biofilms particularly with application in the Industrial (mainly Food industry), Medical (mainly in biomedical devices and implants) and Environmental (mainly marine biofilms) sectors.
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Identification

Personal identification

Full name
Filipe José Menezes Mergulhão

Citation names

  • Mergulhão, Filipe

Author identifiers

Ciência ID
E310-ABDE-8A70
ORCID iD
0000-0001-5233-1037
Scopus Author Id
6506782617

Websites

  • https://sigarra.up.pt/feup/pt/func_geral.formview?p_codigo=403865 (Scholar)
  • lepabe.fe.up.pt/f_mergulhão (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Medical and Health Sciences - Medical Biotechnology - Biomaterials
  • Engineering and Technology - Industrial Biotechnology - Bioproducts Biomaterials

Languages

Language Speaking Reading Writing Listening Peer-review
English Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2002
Concluded
 Engenharia Química (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Design of bacterial vector systems for the secretion of recombinant proteins" (THESIS/DISSERTATION)
 Approved (maximum classification)
1998
Concluded
 Engenharia Química (Licenciatura)
Universidade de Lisboa Instituto Superior Técnico, Portugal
 16/20
Affiliation

Science

Category
Host institution 		Employer
2007 - Current Researcher (Research) Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2005 - Current Assistant Professor (University Teacher) Universidade do Porto Faculdade de Engenharia, Portugal
2012/02/15 - 2012/07/30 Invited Assistant Professor (University Teacher) Stanford University Department of Microbiology and Immunology, United States
2002 - 2003 Assistant Professor (University Teacher) Universidade Lusófona de Humanidades e Tecnologias, Portugal
1998 - 1999 Assistant (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal

Others

Category
Host institution 		Employer
2002 - 2005 Post-doctoral fellow Universidade de Lisboa Instituto Superior Técnico, Portugal
2003 - 2003 Post-doctoral fellow Massachusetts Institute of Technology Department of Chemistry, United States
2003 - 2003 Post-doctoral fellow University of Cambridge, United Kingdom
1998 - 2002 PhD fellow Universidade de Lisboa Instituto Superior Técnico, Portugal
2000 - 2000 PhD fellow Kungliga Tekniska Högskolan, Sweden
1997 - 1997 Erasmus fellow University of St Andrews School of Biology, United Kingdom
Projects

Grant

Designation Funders
2010 - 2013/11 Bioresist - The influence of biofilm phenotype on its resilience and resistance
Provided by PTCRIS: 105085
Researcher
Fundação para a Ciência e a Tecnologia
2010 - 2013/11 SYNBIOBACTHER - Synthetic biology approaches to engineer "therapeutic" bacteria
Provided by PTCRIS: 102863
Researcher
Fundação para a Ciência e a Tecnologia
2010 - 2013/10 Fouling and cleaning of micro channels
Provided by PTCRIS: 105535
Researcher
Fundação para a Ciência e a Tecnologia
2010 - 2013/10 Filmstart - Initial conditions controlling the onset of biofilms
Provided by PTCRIS: 104940
Principal investigator
Fundação para a Ciência e a Tecnologia
2009 - 2012/09 Bridging Systems and Synthetic Biology for the development of Improved Microbial Cell Factories
Provided by PTCRIS: 84035
Researcher
Fundação para a Ciência e a Tecnologia
2007 - 2010/09 The interaction between recombinant protein secretion and biofilm formation and stability
Provided by PTCRIS: 69092
Principal investigator
Fundação para a Ciência e a Tecnologia

Contract

Designation Funders
2018 - Current FIlmprot - The use of Escherichia coli biofilms for the production of recombinant proteins and other added-value compounds
POCI-01-0145-FEDER-029589
Principal investigator
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2018 - Current DelNam - Strengthening the Research Area of Delivery of Nucleic acid mimics (NAMS) into bacteria to fight the antibiotic crisis
810685
810685
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
 N/A
2017 - Current PRINT-AID - Multidisciplinary european training network for development of personalized anti-infective medical devices combining printing technologies and antimicrobial functionality
722467
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2021 - 2024/02/29 NanoCAT - Using modified carbon nanotubes to prevent biofilm formation in urinary catheters and stents
PTDC/CTM-COM/4844/2020
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
2020 - 2023/12 SurfSafe - Surface modification to increase microbial SAFEty in the food industry
952471
Principal investigator
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2015 - 2020/06/30 CVMar+i - Inovação industrial através de colaborações específicas entre empresas e centros de investigação no contexto de valorização biotecnológica marinha
0302_CVMAR_I_1_P
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2019 - 2019/12/31 Projeto Estratégico do LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia - Coordinator of the Biotechnology group
UID/EQU/00511/2019
UID/EQU/00511/2019
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
 N/A
2016 - 2019/09/30 LEPABE-2-ECO-INNOVATION - Coordinator of the Biotechnology group
NORTE-01-0145-FEDER-000005
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2015 - 2019/01/31 Pomacea - Tecnologias económicas para controlo de (bio)fouling em membranas através da otimização de métodos de pré-tratamentos de limpeza
INN-INDIGO/0001/2014
Researcher
2015 - 2018/12/31 Projeto Estratégico - UID/EQU/00511/2013 - Coordinator of the Biotechnology group
UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2014 - 2015/12/31 Programa Incentivo/EQB/UI0511/2014 - Coordinator of the Biotechnology group
Incentivo/EQB/UI0511/2014
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2013 - 2015/10/31 Novomar - Consolidação do Centro Multipolar de Valorização de Recursos Marinhos visando novos usos do mar
0687_NOVOMAR_1_P
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2013 - 2015/08 Phytodisinfectants - Disinfection of planktonic bacteria and biofilms in hospital settings with phytochemical products
Provided by PTCRIS: 125925
Researcher
Fundação para a Ciência e a Tecnologia
2014 - 2015/06/30 (Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health - Coordinator of the Biotechnology group
NORTE-07-0124-FEDER-000025
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2014 - 2015/06/30 Chemical Engineering: Process Optimization and Energy Conversion - Coordinator of the Biotechnology Group
NORTE-07-0124-FEDER-000026
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2013 - 2014/12/31 PROJECTO ESTRATÉGICO - UI 511 - 2013-2014 - Coordinator of the Biotechnology Group
PEst-C/EQB/UI0511/2013
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2012 - 2014/12/31 Susclean - Sustainable Cleaning and Disinfection in Fresh-Cut Food Industries
287514
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2011 - 2012/12/31 PROJECTO ESTRATÉGICO - UI 511 - 2011-2012
FCOMP-01-0124-FEDER-022677
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
Outputs

Publications

Book
  1. Romeu, M.J.; Alves, P.; Morais, J.; Ramos, V.; Vasconcelos, V.; Mergulhão, F.J.. Biofilm formation by a cyanobacterium strain belonging to a distinct leptolyngbya phylotype: Surface effect. 2016.
  2. Mergulhão, Filipe. Biofilms in Bioengineering. Nova Science Publishers. 2013.
  3. Teodósio, J.S.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. The influence of arecombinant plasmid on the formation of Escherichia coli biofilms. 2011.
  4. Mergulhão, F.J.; Monteiro, G.A.. Periplasmic targeting of recombinant proteins in Escherichia coli. 2007.
    10.1007/978-1-59745-466-7_4
Book chapter
  1. Gomes, LucianaCalheiros; Silva, LauraNunes; Simões, Manuel; de Melo, LuísFerreira; Mergulhão, FilipeJosé. "Exploring the Antibiotic Effects in Bacterial Biofilms by Epifluorescence and Scanning Electron Microscopy". edited by Polychroniadis, E. K.; Oral, Ahmet Yavuz; Ozer, Mehmet, 241-248. Springer International Publishing, 2015.
    10.1007/978-3-319-16919-4_31
Conference abstract
  1. Mergulhao, FJM; Monteiro, GA. "The secretion efficiency of the Escherichia coli Sec pathway is dependent on the growth phase but not on protein size". 2005.
Journal article
  1. M.J. Romeu; D. Domínguez-Pérez; D. Almeida; J. Morais; M.J. Araújo; H. Osório; A. Campos; V. Vasconcelos; F.J. Mergulhão. "Quantitative proteomic analysis of marine biofilms formed by filamentous cyanobacterium". Environmental Research 201 (2021): 111566-111566. https://doi.org/10.1016/j.envres.2021.111566.
    10.1016/j.envres.2021.111566
  2. Mergulhão, Filipe. "Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa". Molecules 26 14 (2021): 4378-4378. http://dx.doi.org/10.3390/molecules26144378.
    10.3390/molecules26144378
  3. Luciana C. Gomes; Sara I. Faria; Jesus Valcarcel; José A. Vázquez; Miguel A. Cerqueira; Lorenzo Pastrana; Ana I. Bourbon; Filipe J. Mergulhão. "The Effect of Molecular Weight on the Antimicrobial Activity of Chitosan from Loligo opalescens for Food Packaging Applications". Marine Drugs 19 7 (2021): 384-384. https://doi.org/10.3390/md19070384.
    10.3390/md19070384
  4. Ali Abou-Hassan; Alexandre Barros; Noor Buchholz; Dario Carugo; Francesco Clavica; Petra de Graaf; Julia de La Cruz; et al. "Potential strategies to prevent encrustations on urinary stents and catheters – thinking outside the box: a European network of multidisciplinary research to improve urinary stents (ENIUS) initiative". Expert Review of Medical Devices (2021): https://doi.org/10.1080/17434440.2021.1939010.
    10.1080/17434440.2021.1939010
  5. Sara I. Faria; Rita Teixeira-Santos; Maria J. Romeu; João Morais; Ed de Jong; Jelmer Sjollema; Vítor Vasconcelos; Filipe J. Mergulhão. "Unveiling the Antifouling Performance of Different Marine Surfaces and Their Effect on the Development and Structure of Cyanobacterial Biofilms". Microorganisms (2021): https://doi.org/10.3390/microorganisms9051102.
    10.3390/microorganisms9051102
  6. Marisa Gomes; Luciana C. Gomes; Rita Teixeira-Santos; Manuel F. R. Pereira; Olívia S. G. P. Soares; Filipe J. Mergulhão. "Optimizing CNT Loading in Antimicrobial Composites for Urinary Tract Application". Applied Sciences 11 9 (2021): 4038-4038. https://doi.org/10.3390/app11094038.
    10.3390/app11094038
  7. Olga Ferreira; Patrícia Rijo; João Gomes; Ricardo Santos; Sílvia Monteiro; Rita Guedes; Maria Luísa Serralheiro; et al. "Antimicrobial Ceramic Filters for Water Bio-Decontamination". Coatings 11 3 (2021): 323-323. https://doi.org/10.3390/coatings11030323.
    10.3390/coatings11030323
  8. Sara I. Faria; Luciana C. Gomes; Rita Teixeira-Santos; João Morais; Vítor Vasconcelos; Filipe J. M. Mergulhão. "Developing New Marine Antifouling Surfaces: Learning from Single-Strain Laboratory Tests". Coatings 11 1 (2021): 90-90. https://doi.org/10.3390/coatings11010090.
    10.3390/coatings11010090
  9. Mergulhão, Filipe. "Targeting biofilms in medical devices using probiotic cells: a systematic review". AIMS Materials Science 8 4 (2021): 501-523. http://dx.doi.org/10.3934/matersci.2021031.
    10.3934/matersci.2021031
  10. Mergulhão, Filipe. "Antimicrobial and anti-adhesive properties of carbon nanotube-based surfaces for medical applications: a systematic review.". iScience (2020): https://europepmc.org/articles/PMC7809508.
    10.1016/j.isci.2020.102001
  11. Fábio M. Carvalho; Rita Teixeira-Santos; Filipe J. M. Mergulhão; Luciana C. Gomes. "The Use of Probiotics to Fight Biofilms in Medical Devices: A Systematic Review and Meta-Analysis". Microorganisms (2020): https://doi.org/10.3390/microorganisms9010027.
    10.3390/microorganisms9010027
  12. Mergulhão, Filipe. "Cell adhesion in microchannel multiple constrictions - Evidence of mass transport limitations.". Colloids and surfaces. B, Biointerfaces (2020): https://doi.org/10.1016/j.colsurfb.2020.111490.
    10.1016/j.colsurfb.2020.111490
  13. Andreia S. Azevedo; Gislaine P. Gerola; João Baptista; Carina Almeida; Joana Peres; Filipe J. Mergulhão; Nuno F. Azevedo. "Increased Intraspecies Diversity in Escherichia coli Biofilms Promotes Cellular Growth at the Expense of Matrix Production". Antibiotics (2020): https://doi.org/10.3390/antibiotics9110818.
    10.3390/antibiotics9110818
  14. Mergulhão, Filipe. "Combining chemistry and topography to fight biofilm formation: Fabrication of micropatterned surfaces with a peptide-based coating.". Colloids and surfaces. B, Biointerfaces (2020): https://doi.org/10.1016/j.colsurfb.2020.111365.
    10.1016/j.colsurfb.2020.111365
  15. Sara I. Faria; Rita Teixeira-Santos; Luciana C. Gomes; Elisabete R. Silva; João Morais; Vítor Vasconcelos; Filipe J. M. Mergulhão. "Experimental Assessment of the Performance of Two Marine Coatings to Curb Biofilm Formation of Microfoulers". Coatings (2020): https://doi.org/10.3390/coatings10090893.
    10.3390/coatings10090893
  16. Maria João Leal Romeu; Dany Domínguez-Pérez; Daniela Almeida; João Morais; Alexandre Campos; Vítor Vasconcelos; Filipe J. M. Mergulhão. "Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach". Biofouling (2020): https://doi.org/10.1080/08927014.2020.1795141.
    10.1080/08927014.2020.1795141
  17. Márcia R. Vagos; Marisa Gomes; Joana M. R. Moreira; Olívia S. G. P. Soares; Manuel F. R. Pereira; Filipe J. Mergulhão. "Carbon Nanotube/Poly(dimethylsiloxane) Composite Materials to Reduce Bacterial Adhesion". Antibiotics 9 8 (2020): 434-434. https://doi.org/10.3390/antibiotics9080434.
    10.3390/antibiotics9080434
  18. Patrícia Alves; Joana Maria Moreira; João Mário Miranda; Filipe José Mergulhão. "Analysing the Initial Bacterial Adhesion to Evaluate the Performance of Antifouling Surfaces". Antibiotics 9 7 (2020): 421-421. https://doi.org/10.3390/antibiotics9070421.
    10.3390/antibiotics9070421
  19. P Alves; LC Gomes; M Vorobii; C Rodriguez-Emmenegger; FJ Mergulhão. "The potential advantages of using a poly(HPMA) brush in urinary catheters: effects on biofilm cells and architecture". Colloids and Surfaces B: Biointerfaces (2020): https://doi.org/10.1016/j.colsurfb.2020.110976.
    10.1016/j.colsurfb.2020.110976
  20. Patrícia Alves; Luciana Calheiros Gomes; Cesar Rodríguez-Emmenegger; Filipe José Mergulhão. "Efficacy of A Poly(MeOEGMA) Brush on the Prevention of Escherichia coli Biofilm Formation and Susceptibility". Antibiotics 9 5 (2020): 216-216. https://doi.org/10.3390/antibiotics9050216.
    10.3390/antibiotics9050216
  21. Sara I. Faria; Rita Teixeira-Santos; Maria J. Romeu; João Morais; Vitor Vasconcelos; Filipe J. Mergulhão. "The Relative Importance of Shear Forces and Surface Hydrophobicity on Biofilm Formation by Coccoid Cyanobacteria". Polymers 12 3 (2020): 653-653. https://doi.org/10.3390/polym12030653.
    10.3390/polym12030653
  22. Mergulhão, Filipe. "Magnetron co-sputtered TiO2/SiO2/Ag nanocomposite thin coatings inhibiting bacterial adhesion and biofilm formation". Surface and Coatings Technology (2020): http://dx.doi.org/10.1016/j.surfcoat.2019.125322.
    10.1016/j.surfcoat.2019.125322
  23. Luciana Gomes; Gabriel Monteiro; Filipe Mergulhão. "The Impact of IPTG Induction on Plasmid Stability and Heterologous Protein Expression by Escherichia coli Biofilms". International Journal of Molecular Sciences (2020): https://doi.org/10.3390/ijms21020576.
    10.3390/ijms21020576
  24. Maria J. Romeu; Patrícia Alves; João Morais; João M. Miranda; Ed.D. Jong; Jelmer Sjollema; Vítor Ramos; Vitor Vasconcelos; Filipe J. M. Mergulhão. "Biofilm formation behaviour of marine filamentous cyanobacterial strains in controlled hydrodynamic conditions". Environmental Microbiology 21 11 (2019): 4411-4424. https://doi.org/10.1111/1462-2920.14807.
    10.1111/1462-2920.14807
  25. J Ponmozhi; J. M. R. Moreira; Filipe Mergulhão; J. B. L. M. Campos; Joao Miranda. "Fabrication and Hydrodynamic Characterization of a Microfluidic Device for Cell Adhesion Tests in Polymeric Surfaces". Micromachines (2019): https://www.mdpi.com/2072-666X/10/5/303.
    10.3390/mi10050303
  26. Mergulhão, Filipe. "Evaluating Efficacy of Antimicrobial and Antifouling Materials for Urinary Tract Medical Devices: Challenges and Recommendations". Macromolecular Bioscience (2019): http://dx.doi.org/10.1002/mabi.201800384.
    10.1002/mabi.201800384
  27. Márcia R. Vagos; Joana M.R. Moreira; Olivia S.G.P. Soares; Manuel F.R. Pereira; Filipe J. Mergulhão. "Incorporation of carbon nanotubes in polydimethylsiloxane to control Escherichia coli adhesion". Polymer Composites (2019): https://doi.org/10.1002/pc.25125.
    10.1002/pc.25125
  28. Mergulhão, Filipe. "Recombinant protein expression in biofilms". AIMS Microbiology (2019): http://dx.doi.org/10.3934/microbiol.2019.3.232.
    10.3934/microbiol.2019.3.232
  29. Betina Lopez-Mila; Patrícia Alves; Tomáš Riedel; Barbara Dittrich; Filipe Mergulhão; Cesar Rodriguez-Emmenegger. "Effect of shear stress on the reduction of bacterial adhesion to antifouling polymers". Bioinspiration & Biomimetics 13 6 (2018): 065001-065001. https://doi.org/10.1088/1748-3190/aadcc2.
    10.1088/1748-3190/aadcc2
  30. Mergulhão, Filipe. "The effects of fluid composition and shear conditions on bacterial adhesion to an antifouling peptide-coated surface". MRS Communications (2018): http://dx.doi.org/10.1557/mrc.2018.160.
    10.1557/mrc.2018.160
  31. L.C. Gomes; J. Deschamps; R. Briandet; F.J. Mergulhão. "Impact of modified diamond-like carbon coatings on the spatial organization and disinfection of mixed-biofilms composed of Escherichia coli and Pantoea agglomerans industrial isolates". International Journal of Food Microbiology 277 (2018): 74-82. https://doi.org/10.1016/j.ijfoodmicro.2018.04.017.
    10.1016/j.ijfoodmicro.2018.04.017
  32. Mergulhão, Filipe. "Comparing the Recombinant Protein Production Potential of Planktonic and Biofilm Cells". Microorganisms (2018): http://www.mdpi.com/2076-2607/6/2/48.
    10.3390/microorganisms6020048
  33. L.C. Gomes; J.-C. Piard; R. Briandet; F.J. Mergulhão. "Pseudomonas grimontii biofilm protects food contact surfaces from Escherichia coli colonization". LWT - Food Science and Technology (2017): https://doi.org/10.1016%2Fj.lwt.2017.03.005.
    10.1016/j.lwt.2017.03.005
  34. J.M.R. Moreira; L.C. Gomes; K.A. Whitehead; S. Lynch; L.A. Tetlow; F.J. Mergulhão. "Effect of surface conditioning with cellular extracts on Escherichia coli adhesion and initial biofilm formation". Food and Bioproducts Processing 104 (2017): 1-12. https://doi.org/10.1016%2Fj.fbp.2017.03.008.
    10.1016/j.fbp.2017.03.008
  35. Luciana Calheiros Gomes; Filipe José Mergulhão. "SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment". Scanning (2017):
    10.1155/2017/2960194
  36. Araújo, P.A.; Machado, I.; Meireles, A.; Leiknes, T.; Mergulhão, F.; Melo, L.F.; Simões, M.. "Combination of selected enzymes with cetyltrimethylammonium bromide in biofilm inactivation, removal and regrowth". Food Research International 95 (2017): 101-107. http://www.scopus.com/inward/record.url?eid=2-s2.0-85014941229&partnerID=MN8TOARS.
    10.1016/j.foodres.2017.02.016
  37. Gomes, L.C.; Mergulhão, F.J.. "Heterologous protein production in Escherichia coli biofilms: A non-conventional form of high cell density cultivation". Process Biochemistry 57 (2017): 1-8. http://www.scopus.com/inward/record.url?eid=2-s2.0-85017439544&partnerID=MN8TOARS.
    10.1016/j.procbio.2017.03.018
  38. Meireles, A.; Fulgêncio, R.; Machado, I.; Mergulhão, F.; Melo, L.; Simões, M.. "Characterization of the heterotrophic bacteria from a minimally processed vegetables plant". LWT - Food Science and Technology 85 (2017): 293-300. http://www.scopus.com/inward/record.url?eid=2-s2.0-85010878293&partnerID=MN8TOARS.
    10.1016/j.lwt.2017.01.038
  39. Gomes, L.C.; Mergulhão, F.J.. "Effects of antibiotic concentration and nutrient medium composition on Escherichia coli biofilm formation and green fluorescent protein expression". FEMS Microbiology Letters 364 5 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85018334955&partnerID=MN8TOARS.
    10.1093/femsle/fnx042
  40. Azevedo, A.S.; Almeida, C.; Gomes, L.C.; Ferreira, C.; Mergulhão, F.J.; Melo, L.F.; Azevedo, N.F.. "An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium". Biochemical Engineering Journal 118 (2017): 64-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84998772458&partnerID=MN8TOARS.
    10.1016/j.bej.2016.11.013
  41. Costa, A.M.; Mergulhão, F.J.; Briandet, R.; Azevedo, N.F.. "It is all about location: How to pinpoint microorganisms and their functions in multispecies biofilms". Future Microbiology 12 11 (2017): 987-999. http://www.scopus.com/inward/record.url?eid=2-s2.0-85028548191&partnerID=MN8TOARS.
    10.2217/fmb-2017-0053
  42. Azeredo, J.; Azevedo, N.F.; Briandet, R.; Cerca, N.; Coenye, T.; Costa, A.R.; Desvaux, M.; et al. "Critical review on biofilm methods". Critical Reviews in Microbiology 43 3 (2017): 313-351. http://www.scopus.com/inward/record.url?eid=2-s2.0-85014963888&partnerID=MN8TOARS.
    10.1080/1040841x.2016.1208146
  43. Mergulhão, Filipe. "Surface conditioning with Escherichia coli cell wall components can reduce biofilm formation by decreasing initial adhesion". AIMS Microbiology (2017): http://dx.doi.org/10.3934/microbiol.2017.3.613.
    10.3934/microbiol.2017.3.613
  44. L.C. Gomes; D. Carvalho; R. Briandet; F.J. Mergulhão. "Temporal variation of recombinant protein expression in Escherichia coli biofilms analysed at single-cell level". Process Biochemistry 51 9 (2016): 1155-1161. https://doi.org/10.1016%2Fj.procbio.2016.05.016.
    10.1016/j.procbio.2016.05.016
  45. Gomes IB; Malheiro J; Mergulhão F; Maillard JY; Simões M. "Comparison of the efficacy of natural-based and synthetic biocides to disinfect silicone and stainless steel surfaces.". (2016): http://europepmc.org/abstract/med/26926153.
    10.1093/femspd/ftw014
  46. Araújo, P.A.; Malheiro, J.; MacHado, I.; Mergulhão, F.; Melo, L.; Simões, M.; Araujo, PA; et al. "Influence of flow velocity on the characteristics of pseudomonas fluorescens biofilms". Journal of Environmental Engineering (United States) 142 7 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84975247417&partnerID=MN8TOARS.
    10.1061/(ASCE)EE.1943-7870.0001068
  47. Moreira, J.M.R.; Fulgêncio, R.; Alves, P.; Machado, I.; Bialuch, I.; Melo, L.F.; Simões, M.; Mergulhão, F.J.. "Evaluation of SICAN performance for biofouling mitigation in the food industry". Food Control 62 (2016): 201-207. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946595450&partnerID=MN8TOARS.
    10.1016/j.foodcont.2015.10.023
  48. Moreira, J.M.R.; Fulgêncio, R.; Oliveira, F.; Machado, I.; Bialuch, I.; Melo, L.F.; Simões, M.; Mergulhão, F.J.. "Evaluation of SICON® surfaces for biofouling mitigation in critical process areas". Food and Bioproducts Processing 98 (2016): 173-180. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956686618&partnerID=MN8TOARS.
    10.1016/j.fbp.2016.01.009
  49. Gomes, LC; Mergulhao, FJ. "Effect of heterologous protein expression on Escherichia coli biofilm formation and biocide susceptibility". AIMS MICROBIOLOGY (2016):
    10.3934/microbiol.2016.4.434
  50. Machado, I.; Meireles, A.; Fulgêncio, R.; Mergulhão, F.; Simões, M.; Melo, L.F.. "Disinfection with neutral electrolyzed oxidizing water to reduce microbial load and to prevent biofilm regrowth in the processing of fresh-cut vegetables". Food and Bioproducts Processing 98 (2016): 333-340. http://www.scopus.com/inward/record.url?eid=2-s2.0-84961794771&partnerID=MN8TOARS.
    10.1016/j.fbp.2016.02.008
  51. Moreira, J.M.R.; Ponmozhi, J.; Campos, J.B.L.M.; Miranda, J.M.; Mergulhão, F.J.. "Micro- and macro-flow systems to study Escherichia coli adhesion to biomedical materials". Chemical Engineering Science 126 (2015): 440-445. http://dx.doi.org/10.1016/j.ces.2014.12.054.
    Accepted • 10.1016/j.ces.2014.12.054
  52. Malheiro, J.; Araújo, P.; Machado, I.; Lemos, M.; Mergulhão, F.; Melo, L.; Simões, M.. "The Effects of Selected Brominated and Chlorinated Chemicals on Pseudomonas Fluorescens Planktonic Cells and Flow-Generated Biofilms". Journal of Food Processing and Preservation (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84949257163&partnerID=MN8TOARS.
    10.1111/jfpp.12609
  53. Lemos, M.; Mergulhão, F.; Melo, L.; Simões, M.. "The effect of shear stress on the formation and removal of Bacillus cereus biofilms". Food and Bioproducts Processing 93 (2015): 242-248. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956571746&partnerID=MN8TOARS.
    10.1016/j.fbp.2014.09.005
  54. Lemos, M.; Gomes, I.; Mergulhão, F.; Melo, L.; Simões, M.. "The effects of surface type on the removal of Bacillus cereus and Pseudomonas fluorescens single and dual species biofilms". Food and Bioproducts Processing 93 (2015): 234-241. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956571748&partnerID=MN8TOARS.
    10.1016/j.fbp.2014.08.009
  55. Pogiatzis, T.A.; Vassiliadis, V.S.; Mergulhão, F.J.; Wilson, D.I.. "Choosing when to clean and how to clean biofilms in heat exchangers". Heat Transfer Engineering 36 (2015): 676-684. http://www.scopus.com/inward/record.url?eid=2-s2.0-84910656178&partnerID=MN8TOARS.
    10.1080/01457632.2015.954940
  56. Moreira, J.M.R.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "The combined effects of shear stress and mass transfer on the balance between biofilm and suspended cell dynamics". Desalination and Water Treatment 53 12 (2015): 3348-3354. http://www.scopus.com/inward/record.url?eid=2-s2.0-84923541231&partnerID=MN8TOARS.
    10.1080/19443994.2014.933625
  57. Gomes, L.C.; Silva, L.N.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "Escherichia coli adhesion, biofilm development and antibiotic susceptibility on biomedical materials". Journal of Biomedical Materials Research - Part A 103 4 (2015): 1414-1423. http://www.scopus.com/inward/record.url?eid=2-s2.0-84923575956&partnerID=MN8TOARS.
    10.1002/jbm.a.35277
  58. Moreira, J.M.R.; Gomes, L.C.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "The impact of material properties, nutrient load and shear stress on biofouling in food industries". Food and Bioproducts Processing 95 (2015): 228-236. http://www.scopus.com/inward/record.url?eid=2-s2.0-84934992919&partnerID=MN8TOARS.
    10.1016/j.fbp.2015.05.011
  59. Gomes LC; Moreira JM; Simões M; Melo LF; Mergulhão FJ. "Biofilm localization in the vertical wall of shaking 96-well plates.". (2014): http://europepmc.org/abstract/med/24834360.
    10.1155/2014/231083
  60. Lemos, M.; Borges, A.; Teodósio, J.; Araújo, P.; Mergulhão, F.; Melo, L.; Simões, M.. "The effects of ferulic and salicylic acids on Bacillus cereus and Pseudomonas fluorescens single- and dual-species biofilms". International Biodeterioration and Biodegradation 86 (2014): 42-51. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887023078&partnerID=MN8TOARS.
    10.1016/j.ibiod.2013.06.011
  61. Abreu, A.C.; Borges, A.; Mergulhão, F.; Simoes, M.. "Use of phenyl isothiocyanate for biofilm prevention and control". International Biodeterioration and Biodegradation 86 (2014): 34-41. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887029148&partnerID=MN8TOARS.
    10.1016/j.ibiod.2013.03.024
  62. Meireles, A.; Machado, I.; Fulgêncio, R.; Mergulhão, F.; Melo, L.; Simões, M.. "Efficacy of antimicrobial combinations to reduce the use of sodium hypochlorite in the control of planktonic and sessile Escherichia coli". Biochemical Engineering Journal (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84924912155&partnerID=MN8TOARS.
    10.1016/j.bej.2015.02.035
  63. Gomes, L.C.; Moreira, J.M.R.; Teodósio, J.S.; Araújo, J.D.P.; Miranda, J.M.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "96-well microtiter plates for biofouling simulation in biomedical settings". Biofouling 30 5 (2014): 535-546. http://www.scopus.com/inward/record.url?eid=2-s2.0-84900026049&partnerID=MN8TOARS.
    10.1080/08927014.2014.890713
  64. Moreira, J.M.R.; Araújo, J.D.P.; Miranda, J.M.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "The effects of surface properties on escherichia coli adhesion are modulated by shear stress". Colloids and Surfaces B: Biointerfaces 123 (2014): 1-7. http://www.scopus.com/inward/record.url?eid=2-s2.0-84908539622&partnerID=MN8TOARS.
    10.1016/j.colsurfb.2014.08.016
  65. Araújo, P.A.; Mergulhão, F.; Melo, L.; Simões, M.. "The ability of an antimicrobial agent to penetrate a biofilm is not correlated with its killing or removal efficiency". Biofouling 30 6 (2014): 675-683. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902546839&partnerID=MN8TOARS.
    10.1080/08927014.2014.904294
  66. Moreira, J.M.R.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "Escherichia coli adhesion to surfaces–a thermodynamic assessment". Colloid and Polymer Science 293 1 (2014): 177-185. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925513387&partnerID=MN8TOARS.
    10.1007/s00396-014-3390-x
  67. Araújo PA; Lemos M; Mergulhão F; Melo L; Simões M. "The Influence of Interfering Substances on the Antimicrobial Activity of Selected Quaternary Ammonium Compounds.". (2013): http://europepmc.org/abstract/med/26904590.
    10.1155/2013/237581
  68. Gomes, L.C.; Moreira, J.M.R.; Miranda, J.M.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "Macroscale versus microscale methods for physiological analysis of biofilms formed in 96-well microtiter plates". Journal of Microbiological Methods 95 3 (2013): 342-349. http://www.scopus.com/inward/record.url?eid=2-s2.0-84886809883&partnerID=MN8TOARS.
    10.1016/j.mimet.2013.10.002
  69. Moreira, J.M.R.; Gomes, L.C.; Araújo, J.D.P.; Miranda, J.M.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "The effect of glucose concentration and shaking conditions on Escherichia coli biofilm formation in microtiter plates". Chemical Engineering Science 94 (2013): 192-199. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875583286&partnerID=MN8TOARS.
    10.1016/j.ces.2013.02.045
  70. Teodósio, J.S.; Silva, F.C.; Moreira, J.M.R.; Simões, M.; Melo, L.F.; Alves, M.A.; Mergulhão, F.J.. "Flow cells as quasi-ideal systems for biofouling simulation of industrial piping systems". Biofouling 29 8 (2013): 953-966. http://www.scopus.com/inward/record.url?eid=2-s2.0-84883653767&partnerID=MN8TOARS.
    10.1080/08927014.2013.821467
  71. Moreira, J.M.R.; Teodósio, J.S.; Silva, F.C.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "Influence of flow rate variation on the development of Escherichia coli biofilms". Bioprocess and Biosystems Engineering 36 11 (2013): 1787-1796. http://www.scopus.com/inward/record.url?eid=2-s2.0-84892373974&partnerID=MN8TOARS.
    10.1007/s00449-013-0954-y
  72. Abreu, A.C.; Tavares, R.R.; Borges, A.; Mergulhão, F.; Simões, M.. "Current and emergent strategies for disinfection of hospital environments". Journal of Antimicrobial Chemotherapy 68 12 (2013): 2718-2732. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887514856&partnerID=MN8TOARS.
    10.1093/jac/dkt281
  73. Teodósio, J.S.; Simões, M.; Mergulhão, F.J.. "The influence of nonconjugative Escherichia coli plasmids on biofilm formation and resistance". Journal of Applied Microbiology 113 2 (2012): 373-382. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864005856&partnerID=MN8TOARS.
    10.1111/j.1365-2672.2012.05332.x
  74. Teodósio, J.S.; Simões, M.; Alves, M.A.; Melo, L.F.; Mergulhão, F.J.. "Setup and validation of flow cell systems for biofouling simulation in industrial settings". The Scientific World Journal 2012 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84871938448&partnerID=MN8TOARS.
    10.1100/2012/361496
  75. Teodósio, J.S.; Simões, M.; Melo, L.F.; Mergulhão, F.J.. "Flow cell hydrodynamics and their effects on E. coli biofilm formation under different nutrient conditions and turbulent flow". Biofouling 27 1 (2011): 1-11. http://www.scopus.com/inward/record.url?eid=2-s2.0-78449260750&partnerID=MN8TOARS.
    10.1080/08927014.2010.535206
  76. Carapuça, E.; Azzoni, A.R.; Prazeres, D.M.F.; Monteiro, G.A.; Mergulhão, F.J.M.. "Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR". Molecular Biotechnology 37 2 (2007): 120-126. http://www.scopus.com/inward/record.url?eid=2-s2.0-34248999538&partnerID=MN8TOARS.
    10.1007/s12033-007-0007-3
  77. Mergulhão, F.J.; Monteiro, G.A.. "Analysis of factors affecting the periplasmic production of recombinant proteins in Escherichia coli". Journal of Microbiology and Biotechnology 17 8 (2007): 1236-1241. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548502969&partnerID=MN8TOARS.
  78. Mergulhão, F.J.M.; Summers, D.K.; Monteiro, G.A.. "Recombinant protein secretion in Escherichia coli". Biotechnology Advances 23 3 (2005): 177-202. http://www.scopus.com/inward/record.url?eid=2-s2.0-14844283810&partnerID=MN8TOARS.
    10.1016/j.biotechadv.2004.11.003
  79. Mergulhão, F.J.M.; Monteiro, G.A.. "Secretion capacity limitations of the Sec pathway in Escherichia coli". Journal of Microbiology and Biotechnology 14 1 (2004): 128-133. http://www.scopus.com/inward/record.url?eid=2-s2.0-1542285366&partnerID=MN8TOARS.
  80. Mergulhão, F.J.M.; Monteiro, G.A.; Cabral, J.M.S.; Taipa, M.A.. "Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli". Journal of Microbiology and Biotechnology 14 1 (2004): 1-14. http://www.scopus.com/inward/record.url?eid=2-s2.0-1542285371&partnerID=MN8TOARS.
  81. Mergulhão, F.J.M.; Taipa, M.A.; Cabral, J.M.S.; Monteiro, G.A.. "Evaluation of bottlenecks in proinsulin secretion by Escherichia coli". Journal of Biotechnology 109 1-2 (2004): 31-43. http://www.scopus.com/inward/record.url?eid=2-s2.0-1842425886&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2003.10.024
  82. Mergulhão, F.J.M.; Monteiro, G.A.; Larsson, G.; Bostrom, M.; Farewell, A.; Nyström, T.; Cabral, J.M.S.; Taipa, M.A.. "Evaluation of inducible promoters on the secretion of a ZZ-proinsulin fusion protein in Escherichia coli". Biotechnology and Applied Biochemistry 38 1 (2003): 87-93. http://www.scopus.com/inward/record.url?eid=2-s2.0-0042591085&partnerID=MN8TOARS.
    10.1042/BA20030043
  83. Mergulhão, F.J.M.; Monteiro, G.A.; Larsson, G.; Sandén, A.M.; Farewell, A.; Nystrom, T.; Cabral, J.M.S.; Taipa, M.A.. "Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB". Applied Microbiology and Biotechnology 61 5-6 (2003): 495-501. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037899027&partnerID=MN8TOARS.
    10.1007/s00253-003-1232-8
  84. Mergulhão, F.J.M.; Monteiro, G.A.; Cabral, J.M.S.; Taipa, M.A.. "A quantitative ELISA for monitoring the secretion of ZZ-fusion proteins using SpA domain as immunodetection reporter system". Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology 19 3 (2001): 239-244. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035168991&partnerID=MN8TOARS.
    10.1385/mb:19:3:239
  85. Mergulhao, F.J.M.; Monteiro, G.A.; Kelly, A.G.; Taipa, M.A.; Joaquim, M.S.C.. "Recombinant human proinsulin: A new approach in gene assembly and protein expression". Journal of Microbiology and Biotechnology 10 5 (2000): 690-693. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033757725&partnerID=MN8TOARS.
  86. Mergulhão, F.J.M.; Kelly, A.G.; Monteiro, G.A.; Taipa, M.A.; Cabral, J.M.S.. "Troubleshooting in gene splicing by overlap extension: A step-wise method". Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology 12 3 (1999): 285-287. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033428016&partnerID=MN8TOARS.
    10.1385/mb:12:3:285

Other

Other output
  1. Gomes, LC; Monteiro, GA; Mergulhão, FJ. 2020. Recombinant Protein Production and Plasmid Stability in Escherichia coli Biofilms.
    10.5194/biofilms9-65
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2013/07/22 - Current Evaluation of bacterial adhesion on carbon nanotube-poly (dimethysiloxane) composite materials
Supervisor
 Bioengenharia (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2013/07/08 - Current Biofouling and cleaning of microchannels
Co-supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2011/10/09 - Current Biofilm control with antimicrobial agents: the role of the exopolymeric matrix
Co-supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2019 - 2019 Optimizing Recombinant Protein Production in Escherichia coli biofilms
Supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2019 - 2019 Marine biofilm formed by coccoid cyanobacteria: Development and Control
Supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2019 - 2019 Effects of Dedicated Yeasts as Starter Culture Candidates on the Microbial Community and Metabolite Profiles of Cocoa Pulp-Bean Mass Fermentation Processes
Supervisor
 Bioengenharia (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2017 - 2017 Estudo Comparativo de Métodos de Doseamento de Proteína em Preparações de Imunoglobulina Humana Intravenosa
Supervisor
 Engenharia Química (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2017 - 2017 Characterization of marine biofilms formed by filamentous cyanobacteria
Supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2017 - 2017 Evaluation of Escherichia coli biofilms in controlled hydrodynamic conditions using flow cells
Supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2016 - 2016 Cloning and expression of a new ketoreductase from the marine bacteria Virgibacillus pantothenticus
Supervisor
 Bioengenharia (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2015 - 2015 Avaliação da influência de células humanas e não-humanas no desenvolvimento de linhas celulares estáveis para produção de TribodyTM5
Supervisor
 Engenharia Química (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2015 - 2015 Marine biofilms - how do they start?
Supervisor
 Bioengenharia (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2013 - 2013 The effect of biofilm formation on recombinant protein expression
Supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2012 - 2012 Analysis of factors controlling the onset of bacterial biofilms
Supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2011 - 2011 The interaction between recombinant protein production and biofilm formation and stability
Supervisor
 Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
Distinctions

Award

2011 Fulbright Scholar Program
Fulbright Association, United States