João Paquete-Ferreira is a biochemist specialising in structural biology. He obtained a BSc in Biochemistry in 2017, an MSc in Biochemistry in 2019, and a PhD in Biochemistry in 2025, all awarded by the NOVA School of Science and Technology (NOVA FCT). His main scientific interests focus on protein production for structural determination using X-ray crystallography and cryo-electron microscopy (cryo-EM), the biophysical characterisation of protein–ligand interactions, and the application of structural biology approaches to rational drug development. Since the end of his BSc, Paquete-Ferreira has carried out his research activity at the Macromolecular Crystallography Laboratory of UCIBIO (NOVA FCT), where he acquired extensive experience in protein overexpression, purification, crystallisation, and biophysical characterisation using a wide range of experimental techniques. During his MSc thesis, he solved the first reported three-dimensional structure of a LytR–CpsA–Psr (LCP) protein from Streptococcus dysgalactiae subsp. dysgalactiae and performed an in-depth analysis of its interaction with multiple ligands. His PhD work built directly upon the knowledge and expertise developed during his master’s research. During this period, Paquete-Ferreira characterised an additional LCP protein from S. dysgalactiae subsp. dysgalactiae and investigated the molecular determinants governing ligand recognition within this protein family. This work led to the identification of ADP as a substrate for LCP proteins. He subsequently contributed to the development of a ^1H NMR-based method to monitor ADP hydrolysis. Using the crystal structure of the LytR protein, he also carried out virtual screening studies, identifying ten promising small molecules as putative inhibitors of the LCP protein family. Alongside his research activities, Paquete-Ferreira was involved in the tutoring and co-supervision of BSc and MSc students, reflecting increasing responsibility and academic maturity. Scientific communication was a central component of his training, resulting in five oral communications and five poster presentations at national and international conferences. In parallel, he participated in several internationally recognised training schools and scientific workshops, including CCP4 Study Weekends (2020–2024), Diamond Light Source SAXS workshops (2020), and the Macromolecular Crystallography and Cryo-EM School (2023). Beyond technical training, these events fostered interaction with researchers at different career stages and contributed to knowledge exchange and community building within the structural biology field. Paquete-Ferreira’s activities extend beyond the laboratory environment. He has been actively involved in academic organisations during both his undergraduate and doctoral studies, contributing to initiatives aimed at improving the academic experience of students. Taken together, his experience, technical expertise, and commitment to research and academic engagement make him a valuable asset to a research team in structural biology and related fields.