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In the last 5 years, Sandra's research work has abetted the design of novel platforms for the purification and application of several health-related biomolecules. Until June 2002, Sandra Bernardo was a post-doctoral researcher at the Health Sciences Research Centre in the University of Beira Interior (CICS-UBI), under the project - PUREmiRSILs: Ionic Liquid-based supports for pre-miRNAs purification targeting Alzheimer's disease - in which she has been collaborated in the supervision of MSc and PhD students/applicants. Currently she is the NMR lab technician at CICS-UBI and in the last months, had gathered additional experience in equipment maintenance, lab management and NMR sample preparation, acquisition, and processing, being able to attend 2 excellent courses on this field, watching Bruker webinars online and helping students to perform their experiments. Sandra studied Biochemistry at the Faculty of Sciences at the University of Lisbon, Portugal. In 2013, she completed a MSc degree in Biotechnology by the Technical University of Lisbon, where she had gain knowledge and laboratorial experience on protein purification techniques and monoclonal antibodies manufacturing industry, by working at BioSeparations Lab from BioEngeneering Research Group, IST-UTL, to carry out her master thesis work, which consisted on purifying monoclonal antibodies by aqueous-two phase systems using a dual-affinity ligand molecule (LYTAG-Protein A). From March 2014 to February 2018, S.C. Bernardo joined a PhD scholarship granted by European Research Council Grant (ERC-2013-StG-337753) in Chemical Engineering Doctoral program at the University of Aveiro, Portugal. During almost 5 years, she worked as a research grantee under the framework of the European Research Council project - IgYPurTech - IgY Technology: A Purification Platform using Ionic-Liquid-Based Aqueous Biphasic Systems. Her work involved the development of different purification strategies for polyclonal antibodies from egg yolk. Due to the outstanding results regarding the developed purification process, a patent application (PT115028) was requested to INPI in 2018 (entitled Processo para purificar um anticorpo a partir da gema de ovo) and granted in 2021. In 2019, a patent application (PCT/IB2019/057993) was filed to WIPO, whereas a family of patent applications were requested (WO2020058936, US20210253632, EP3854813 and JP2022501375). Besides this acquired experience in the patent submission, being one of the inventors in an international patent application, in the last 5 years Sandra also published, as first author, 2 original articles, 2 book chapters and 1 review article. Additionally, she was the first author presenting in 4 oral communications in relevant conferences in the field (ESBES, ECAB, ISPPP). She has also collaborated in 4 projects: FP7/2007-2013/ERC-Grant-337753, 4318/40851YL and POCI-01-0247-FEDER-007678 at CICECO-UA; and POCI-01-0145-FEDER-029496 at CICS-UBI. Recently, the candidate has been added to the team of CICS-UBI, participating institution of a consortium in a EIC Pathfinder project recently approved (Yscript-101047214). In addition, in the last 5 years she participated in an intensive entrepreneurship Program, promoted by COTEC, at Porto Business School, where her PurAvTec team presented a business idea (Anticorpos de aves: uma solução para salvar vidas) based on the patented purification technology. Also, in 2018 the project PurAvTec was distinguished in the Health category, upon a pitch presentation (entitled Desenvolvimento de um produto eficaz no combate a bactérias resistentes a antibióticos) at Born from knowledge Ideas program promoted by ANI.
Identification

Personal identification

Full name
Sandra Cristina da Silva Bernardo

Citation names

  • Bernardo, Sandra C.

Author identifiers

Ciência ID
0616-8D07-D7B0
ORCID iD
0000-0003-2872-3609
Scopus Author Id
57210790657

Email addresses

  • sandracsbernardo@gmail.com (Professional)

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
  • Engineering and Technology - Chemical Engineering

Languages

Language Speaking Reading Writing Listening Peer-review
English Intermediate (B1) Advanced (C1) Advanced (C1) Intermediate (B1)
German Beginner (A1) Beginner (A1)
Education
Degree Classification
2022/07/04 - 2022/07/08
Attended
15 th CERMAX practical course on basic NMR (Outros)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2022/06/19 - 2022/06/24
Attended
XIV Manuel Rico NMR Summer School (Outros)
Real Sociedad Espanola de Quimica, Spain
2018/10/19
Attended
Ionic liquids synthesis course (synthesis of novel glycine-betaine-based ILs) (Outros)
Institut de Chimie Moléculaire de Reims, France
2014/03/01 - 2018/09/26
Concluded
Engenharia Química (Doutoramento)
Major in Bioengenharia
Universidade de Aveiro, Portugal
"Purification strategies for polyclonal antibodies from egg yolk" (THESIS/DISSERTATION)
2017/03 - 2017/07
Attended
COHiTEC entrepreneurship Program (Outros)
Major in team “PurAvTec” presenting “Anticorpos de aves: uma solução para salvar vidas”
Universidade do Porto Porto Business School, Portugal
2015/05/28
Attended
HINT Training School: “bottom–up approaches of hybrid materials preparation and design" (Outros)
Institut Jožef Stefan, Slovenia

PARALAB, Portugal
2014/12
Attended
Alemão (A.1) (Outros)
Universidade de Aveiro Departamento de Línguas e Culturas, Portugal
2014/05/27
Attended
workshop: “Técnicas para Caracterização de Nanopartículas” (Outros)
PARALAB, Portugal
2013
Concluded
Biotecnologia (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"LYTAG-driven Integrative Platform for Purification of Monoclonal Antibodies by Aqueous Two-phase Systems" (THESIS/DISSERTATION)
17
2011/07
Concluded
Bioquímica (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
Affiliation

Science

Category
Host institution
Employer
2019/06 - 2022/06 Researcher (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2019/02/01 - 2019/06 Postdoc (Research) Universidade de Aveiro CICECO, Portugal
2014/03/01 - 2018/09/26 Researcher (Research) Universidade de Aveiro CICECO, Portugal

Other Careers

Category
Host institution
Employer
2022/06/03 - 2023/07 Técnico Superior (Técnico Superior) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Projects

Grant

Designation Funders
2019/06 - 2022 PUREmiRSILs - Ionic Liquid-based supports for pre-miRNAs purification targeting Alzheimer's disease
POCI-01-0145-FEDER-029496
Researcher
Ongoing
2019/02 - 2019/06 “Smart Green Homes” Project, a co-promotion between Bosch Termotecnologia S.A. and the University of Aveiro
POCI-01-0247-FEDER-007678
Post-doc Fellow
2014/03/01 - 2019/01/31 IGY TECHNOLOGY: A PURIFICATION PLATFORM USING IONIC-LIQUID-BASED AQUEOUS BIPHASIC SYSTEMS
info:eu-repo/grantAgreement/FCT/SFRH/SFRH2%FBD2%F852482%F2012/PT
PhD Student Fellow
Universidade de Aveiro CICECO, Portugal
Concluded

Other

Designation Funders
2022 - Current Yscript: YEAST CELL FACTORY FOR mRNA bioproduction
Research Fellow
Ongoing
2018 - 2018 Extraction and purification of RuBisCo: from waste product to high-value functional protein, under PHC PESSOA 2018
4318/40851YL
Researcher
Universidade de Aveiro, Portugal

Institut de Chimie Moléculaire de Reims, France
Concluded
Outputs

Publications

Book chapter
  1. Bernardo, Sandra C.; Neves, Márcia C.; Freire, Mara G.; Tavares, Ana P.M.. "Applications of hybrid nanoparticles to improve environmental monitoring". In Nano-Bioremediation : Fundamentals and Applications, 147-172. Elsevier, 2022.
    Published
  2. Bernardo, S.C.; Neves, M.C.; Freire, M.G.; Tavares, A.P.M.. "Applications of hybrid nanoparticles to improve environmental monitoring". 147-172. 2021.
  3. Bernardo, Sandra; Sousa, Ana C.; Neves, Márcia C.; Mara G. Freire. "Use of Nanomaterials in the Pretreatment of Water Samples for Environmental Analysis". 2019.
    10.1007/978-981-13-9833-9_6
Conference poster
  1. Sérgio Nunes; A. Rita Carapito; Riscado, Micaela; Bernardo, Sandra C.; Fani Pereira de Sousa. "Purification of pre-miRNA-29 with ionic liquids modified supports". Paper presented in CICS-UBI SYMPOSIUM 2022, 2022.
  2. Ana R. Carapito; Bernardo, Sandra C.; Neves, Márcia C.; Mara G. Freire; Fani Pereira de Sousa. "RNA PURIFICATION USING NOVEL IMIDAZOLIUM-BASED CHROMATOGRAPHIC SUPPORT". Paper presented in XVI ANNUAL CICS-UBI SYMPOSIUM 2021, 2021.
  3. Neves, Márcia C.; Santos, Maria J.; Lobo-Sousa, Guilherme; Bernardo, Sandra C.; Rocha, Luciana; Almeida, Hugo F.; Mara G. Freire. "Supported ionic liquids for the removal of pharmaceuticals from aqueous solutions". Paper presented in ILSEPT 2019, 4th International Conference on Ionic Liquids in Separation and Purification Technology, 2019.
  4. Bernardo, Sandra C.; Coutinho, Joao A. P.; Mara G. Freire. "Aqueous Biphasic Systems Composed of Cholinium-based Ionic Liquids for the Extraction of Immunoglobulin Y, IgY, from Egg Yolk". Paper presented in ESIB 2017 - European Summit of Industrial Biotechnology, 2017.
  5. Bernardo, Sandra C.; Brandenbusch, C.; Held, C.; Sadowski, G; Coutinho, Joao A. P.; Mara G. Freire. "Partition Behaviour of Immunoglobulin Y in Aqueous Biphasic Systems Composed of Ionic Liquids". Paper presented in 26th EUCHEM Conference on Molten Salts and Ionic Liquids, 2016.
  6. Francisco, R.; Tavares, A. P. M.,; Bernardo, Sandra C.; Laranjeira, J.; Curto, P.; Simões, I.; Ribeiro, T. D.; et al. "Avian IgY antibodies: robust tools in research, diagnostics and therapy". Paper presented in 1st International Biomedical Engineering Meeting, 2016.
  7. Bernardo, Sandra C.; Ferreira, F.; Coutinho, Joao A. P.; Mara G. Freire. "Aqueous Biphasic Systems Composed of Cholinium-Based Ionic Liquids for the Extraction of Immunoglobulin Y, Igy, from Egg Yolk". Paper presented in 7th annual PEGS Europe Protein & Antibody Engineering Summit, 2015.
  8. Neves, Márcia C.; Antunes, Joana; Bernardo, Sandra C.; Coutinho, Joao A. P.; Mara G. Freire. "Supported ionic liquids for the purification of immunoglobulin Y (IgY)". Paper presented in IMIL 2015, 2015.
Journal article
  1. Ferreira, P.; Riscado, M.; Bernardo, S.; Freire, M.G.; Faria, J.L.; Tavares, APM.; Silva, CG.; Sousa, F.. "Pristine Multi-walled carbon nanotubes for a rapid and efficient plasmid DNA clarification". Separation and Purification Technology 320 (2023): 124224. http://dx.doi.org/10.1016/j.seppur.2023.124224.
    10.1016/j.seppur.2023.124224
  2. Carapito, Rita; Bernardo, Sandra C.; Pereira, Matheus M.; Neves, Márcia C.; Freire, Mara G.; Sousa, Fani. "Multimodal ionic liquid-based chromatographic supports for an effective RNA purification". Separation and Purification Technology 315 (2023): 123676. http://dx.doi.org/10.1016/j.seppur.2023.123676.
    Published • 10.1016/j.seppur.2023.123676
  3. Bernardo, Sandra C.; Carapito, Rita; Neves, Márcia C.; Freire, Mara G.; Sousa, Fani. "Supported Ionic Liquids Used as Chromatographic Matrices in Bioseparation - An Overview". Molecules 27 5 (2022): 1618. http://dx.doi.org/10.3390/molecules27051618.
    Open access • Published • 10.3390/molecules27051618
  4. Bernardo, Sandra C.; Capela, Emanuel V.; Pereira, Jorge F. B.; Sónia P. M. Ventura; Mara G. Freire; Coutinho, Joao A. P.. "Opposite Effects Induced by Cholinium-Based Ionic Liquid Electrolytes in the Formation of Aqueous Biphasic Systems Comprising Polyethylene Glycol and Sodium Polyacrylate". Molecules 26 21 (2021): 6612. http://dx.doi.org/10.3390/molecules26216612.
    Open access • Published • 10.3390/molecules26216612
  5. Bernardo, Sandra C.; Araújo, Beatriz R.; Sousa, Ana C. A.; Barros, Rafael A.; Cristovão, Ana C.; Neves, Márcia C.; Freire, Mara G.. "Supported Ionic Liquids for the Efficient Removal of Acetylsalicylic Acid from Aqueous Solutions". European Journal of Inorganic Chemistry 2020 24 (2020): 2380-2389. http://dx.doi.org/10.1002/ejic.202000101.
    Published • 10.1002/ejic.202000101
  6. Bernardo, S.C.; Azevedo, A.M.; Aires-Barros, M.R.. "Integrated platforms for the clarification and capture of monoclonal antibodies | Plataformas integrales para la clarificaciony captura de anticuerpos monoclonales". Revista Mexicana de Ingeniera Qumica 13 2 (2014): 349-357. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904279317&partnerID=MN8TOARS.

Intellectual property

Patent
  1. Mara G. Freire; Bernardo, Sandra C.; Coutinho, Joao A. P.. 2021. "PROCESSO PARA PURIFICAR UM ANTICORPO A PARTIR DA GEMA DE OVO, SEUS PRODUTOS E USOS". Portugal.
    Granted/Issued
  2. Mara G. Freire; Bernardo, Sandra C.; Coutinho, Joao A. P.. 2020. "Process for purifying an antibody from egg yolk, products and uses thereof".
    Pending
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/11/09 Design of specific ligands for pre-miRNA purification. ISPPP2021 – 40th International Symposium on the Separation of Proteins, Peptides & Polynucleotides
(Oporto, Portugal)
2021/09/30 DESIGN OF DEEP EUTECTIC SOLVENTS TO IMPROVE RNA STABILIZATION AND STORAGE XVI ANNUAL CICS-UBI SYMPOSIUM 2021
(Covilhã (online event), Portugal)
2021/05/05 Purification of RNAs using novel Ionic liquid-based chromatographic supports ESBES2021- 13th European Symposium on Biochemical Engineering Sciences 2021
ESBES (online event, Slovenia)
2019/09 Removal of the imidacloprid pesticide from water samples using supported ionic liquids (silps) as adsorbents 5th European Congress of Applied Biotechnology
(Florence, Italy)
2019/09 Removal of pharmaceuticals with supported ionic liquids 5th European Congress of Applied Biotechnology
(Florence, Italy)
2017/11 Egg Yolk as a Novel Source of Antibodies and their Applications ESIB- European Summit of Industrial Biotechnology
acib GmbH (Graz, Austria)
2017 Antibodies purification using three-phase partitioning (TPP) strategies ABC2 - The Anything but Conventional Chromatography
(Lisbon, Portugal)
2016 Polymer-Polymer Aqueous Biphasic Systems with Cholinium-based Electrolytes for The Purification of Immunoglobulin Y European Symposium on Biochemical Engineering Sciences
(Dublin, Ireland)
2015 Functionalized-Silica Materials for the Purification of Immunoglobulin Y (Igy) 3rd European Congress of Applied Biotechnology ECAB
(Nice, France)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2021/09 - 2022 Purificação de pre-miRNA-29 com suportes modificados com líquidos iónicos
Co-supervisor
Biotechnology (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2020/10 - 2022 Polímeros para encapsulamento de ácidos nucléicos
Co-supervisor
Ciências Biomédicas (Master)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2019/02/03 - 2019/07 Extração de IgG a partir de soro humano usando sistemas aquosos bifásicos constituídos por líquidos iónicos
Supervisor
Bioquímica (Degree)
Universidade de Aveiro, Portugal

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2023/04 Formulação de nanopartículas para terapia do cancro de pulmão
(Thesis) Main arguer
Karol Brigitte Moreira Jiménez (Master)
Universidade da Beira Interior, Portugal
2023/03 "PolyThymine cryogels as a novel approach to the purification of mRNA encoding anti- ADAM17 antibodies"
(Thesis) Main arguer
Bruno Daniel Lopes Rodrigues. (Master)
Universidade da Beira Interior, Portugal
2021/02/19 Removal of albumin from platelet lysates for the fabrication of hydrogels for 3D cell culture
(Thesis) Main arguer
ANA MIGUEL DA SILVA LOUREIRO (Master)
Universidade de Aveiro Departamento de Química, Portugal
2019/12/19 Produção e purificação de anticorpos monoclonais humanos anti-interleucina-8
(Thesis) Main arguer
JÉSSICA CHAVES BRAGA BAIROS (Master)
Universidade de Aveiro, Portugal
Distinctions

Award

2018 PurAvTec - IgY Purification Technologies, Born from Knowledge Ideas - best idea in health category
Agência Nacional de Inovação SA, Portugal