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Identificação

Identificação pessoal

Nome completo
Margarida Alexandra Vaz Caldeira

Identificadores de autor

Ciência ID
3016-85A2-C9B5
ORCID iD
0000-0001-5107-1499

Domínios de atuação

  • Ciências Médicas e da Saúde - Biotecnologia Médica - Tecnologias que envolvem a Manipulação de Células,Tecidos,Órgãos ou todo o Organismo
  • Ciências Naturais - Ciências Biológicas - Biologia Celular
  • Ciências Naturais - Ciências Biológicas - Biologia Molecular
Percurso profissional

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento
Empregador
2011 - 2013 Professor Auxiliar Convidado (Docente Universitário) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2008/02 - 2008/08 Professor Auxiliar Convidado (Docente Universitário) Universidade de Coimbra - Departamento de Zoologia, Portugal
Universidade de Coimbra - Departamento de Zoologia, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2019/01 - Atual Doctorate Investigator (DL57) within the project UID/NEU/04539/2013: nº 30 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2015 - 2018 PostDoctoral Fellow - Microscopy Unit (RD Units Strategic Plan: POCI-01-0145-FEDER-007440) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2013 - 2015 PostDoctoral Fellow - Microscopy Unit (CENTRO-07-0224-FEDER-002006: Aging, Stress and Chronic Diseases: From Mechanisms to Therapeutics) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2007/06 - 2011 PostDoctoral Fellow (Mechanisms of regulation of the proteasome activity under excitotoxic conditions)- SFRH/BPD/30432/2006 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Oftalmologia e Ciências da Visão, Portugal
(...)
2002 - 2007 PhD Student Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2001/09/03 - 2002/08/30 Undergraduate Training Student Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
CNS Discovery Research, Janssen Pharmaceutica N. V., a division of Johnson & Johnson Pharmaceutical Research & Development, Bélgica
Produções

Publicações

Artigo em revista
  1. Diana Costa; Carolina Costa; Margarida Caldeira; Luísa Cortes; João A. Queiroz; Carla Cruz. "Targeting of Cellular Organelles by Fluorescent Plasmid DNA Nanoparticles". Biomacromolecules 18 9 (2017): 2928-2936. https://doi.org/10.1021/acs.biomac.7b00877.
    10.1021/acs.biomac.7b00877
  2. Salazar, Ivan; Mele, Miranda; Caldeira, Margarida; Costa, Rui; Correia, Bárbara; Frisari, Simone; Duarte, Carlos. "Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons". BIO-PROTOCOL 7 18 (2017): http://dx.doi.org/10.21769/bioprotoc.2551.
    10.21769/bioprotoc.2551
  3. Caldeira, M.V.; Salazar, I.L.; Curcio, M.; Duarte, C.B.. "The role of proteases in hippocampal synaptic plasticity: Putting together small pieces of a complex puzzle". Neurochemical Research 41 1-2 (2016): 156-182. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946811163&partnerID=MN8TOARS.
    10.1007/s11064-015-1752-5
  4. Caldeira, Margarida; Ferreira, Joana; Carvalho, Ana; Duarte, Carlos. "Biotinylation and Purification of Plasma Membrane-associated Proteins from Rodent Cultured Neurons". BIO-PROTOCOL 6 10 (2016): http://dx.doi.org/10.21769/bioprotoc.1807.
    10.21769/bioprotoc.1807
  5. Salazar IL; Caldeira MV; Curcio M; Duarte CB. "The Role of Proteases in Hippocampal Synaptic Plasticity: Putting Together Small Pieces of a Complex Puzzle.". (2015): http://europepmc.org/abstract/med/26547197.
  6. Caldeira MV; Salazar IL; Curcio M; Canzoniero LM; Duarte CB. "Role of the ubiquitin-proteasome system in brain ischemia: friend or foe?". (2014): http://europepmc.org/abstract/med/24157661.
    10.1016/j.pneurobio.2013.10.003
  7. Caldeira MV; Curcio M; Leal G; Salazar IL; Mele M; Santos AR; Melo CV; et al. "Excitotoxic stimulation downregulates the ubiquitin-proteasome system through activation of NMDA receptors in cultured hippocampal neurons.". (2013): http://europepmc.org/abstract/med/23069389.
    10.1016/j.bbadis.2012.10.009
  8. Baptista MS; Melo CV; Armelão M; Herrmann D; Pimentel DO; Leal G; Caldeira MV; et al. "Role of the proteasome in excitotoxicity-induced cleavage of glutamic acid decarboxylase in cultured hippocampal neurons.". (2010): http://europepmc.org/abstract/med/20405034.
    10.1371/journal.pone.0010139
  9. Santos SD; Carvalho AL; Caldeira MV; Duarte CB. "Regulation of AMPA receptors and synaptic plasticity.". (2009): http://europepmc.org/abstract/med/18424006.
    10.1016/j.neuroscience.2008.02.037
  10. Carvalho AL; Caldeira MV; Santos SD; Duarte CB. "Role of the brain-derived neurotrophic factor at glutamatergic synapses.". (2008): http://europepmc.org/abstract/med/18059328.
    10.1038/sj.bjp.0707509
  11. Caldeira MV; Melo CV; Pereira DB; Carvalho RF; Carvalho AL; Duarte CB. "BDNF regulates the expression and traffic of NMDA receptors in cultured hippocampal neurons.". (2007): http://europepmc.org/abstract/med/17428676.
    10.1016/j.mcn.2007.02.019
  12. Caldeira MV; Melo CV; Pereira DB; Carvalho R; Correia SS; Backos DS; Carvalho AL; Esteban JA; Duarte CB. "Brain-derived neurotrophic factor regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits in hippocampal neurons.". (2007): http://europepmc.org/abstract/med/17337442.
    10.1074/jbc.M700607200
  13. Caldeira, M.V.; Melo, C.V.; Pereira, D.B.; Carvalho, R.; Correia, S.S.; Backos, D.S.; Carvalho, A.L.; Esteban, J.A.; Duarte, C.B.. "Erratum: Brain-derived neurotrophic factor regulates the expression and synaptic delivery of a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits in hippocampal neurons (Journal of Biological Chemistry (2007) 282, (12619-12628))". Journal of Biological Chemistry 282 37 (2007): http://www.scopus.com/inward/record.url?eid=2-s2.0-34848900818&partnerID=MN8TOARS.
Capítulo de livro
  1. Carvalho, A. L.; Caldeira, M. V.; Gomes, A. R.; Carvalho, A. P.; Duarte, C. B.. "Regulation of AMPA Receptors by Metabotropic Receptors and Receptor Tyrosine Kinases: Mechanisms and Physiological Roles". In Handbook of Neurochemistry and Molecular Neurobiology, 275-323. Springer US, 2008.
    10.1007/978-0-387-30382-6_12
Poster em conferência
  1. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia". Trabalho apresentado em EMBO Workshop on Proteostasis: from organelles to organisms, 2019.
  2. Ivan L Salazar; Frisari., S.; Caldeira, Margarida; Duarte, Carlos B.. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia.". Trabalho apresentado em 1st Neuroscience and Disease Retreat from the Center of Neuroscience and Cell Biology, 2019.
  3. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemi". Trabalho apresentado em XVII SINS National Congress, Italy, 2017.
  4. "Interplay between the Ubiquitin-Proteasome System and calpains in brain ischemia". Trabalho apresentado em ISN-ESN, Paris, France, 2017.
  5. "Brain ischemia-induced alterations in the ubiquitin-proteasome system: role in neuronal demise". Trabalho apresentado em 7th ISN Special Neurochemistry Conference- Synaptic Function and Dysfunction In Brain Diseases, Portugal, 2016.
  6. "Excitotoxic glutamate stimulation and oxygen and glucose deprivation downregulate the 26S proteasome in cultured neurons.". Trabalho apresentado em 9th FENS Forum of Neuroscience, 2014.
  7. "Glutamate receptor activation is coupled to 26S proteasome downregulation in in vitro ischemia". Trabalho apresentado em 5th Conference on Advances in molecular mechanisms underlying neurological disorders, 2013.
  8. Ivan L Salazar; Margarida V. Caldeira; Michele Curcio; Duarte, Carlos B.. "26S Proteasome downregulation under excitotoxic conditions". Trabalho apresentado em X CNC Annual Meeting, 2012.
  9. "Excitotoxic stimulation-induced changes in the ubiquitin-proteasome system in neuronal hippocampal cultures". Trabalho apresentado em Society for Neuroscience (SfN) 36th Annual Meeting, 2009.
  10. "Changes in the ubiquitin-proteasome system associated with neuronal damage in brain ischemia". Trabalho apresentado em XVI National Congress of Biochemistry, 2008.
  11. "BDNF differentially regulates the expression, delivery and activity of AMPA and NMDA receptor subunits in cultured hippocampal neurons". Trabalho apresentado em 10th Meeting of the Portuguese Society for Neurosciences, 2007.
  12. "BDNF regulates differentially the expression and delivery of NMDA receptor subunits in cultured hippocampal neurons". Trabalho apresentado em XV National Congress of Biochemistry, Aveiro, Portugal, 2006.
  13. "BDNF regulates the expression and traffic of NMDA receptor in cultured hippocampal neurons". Trabalho apresentado em Society for Neuroscience (SfN) 36th Annual Meeting, Atlanta, E.U.A., 2006.
  14. "Modulation of AMPA receptors expression and synaptic delivery by BDNF in hippocampal neurons". Trabalho apresentado em Society for Neuroscience (SfN) 35th Annual Meeting, Washington, DC, U.S.A., 2005.
  15. "BDNF regulates the expression of AMPA receptor subunits in hippocampal neurons". Trabalho apresentado em 9th Meeting of the Portuguese Society for Neurosciences, Luso, Portugal, 2005.
  16. "Regulation of the expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits by brain derived neurotrophic factor in cultured hippocampal neurons". Trabalho apresentado em Society for Neuroscience (SfN) 34th Annual Meeting, San Diego, U.S.A., 2004.
  17. "Effect of BDNF on the expression of AMPA receptor subunits in cultured hippocampal neurons.". Trabalho apresentado em 4th Forum of European Neuroscience (FENS), Lisbon, Portugal, 2004.
  18. "Novel mGlu1 receptor antagonists are potent antinociceptive agents in animal models involving sensitization of nociception and hyperalgesia: a comparison with reference mGlu1/5 antagonists and morphine". Trabalho apresentado em 8th Meeting of the Portuguese Society for Neurosciences, Curia, Portugal, 2003.
  19. "Recently discovered novel mGlu1 receptor antagonists are potent antinociceptive agents in the second phase of the formalin test: a comparison with mGlu1/5 reference antagonists and morphine". Trabalho apresentado em XIII National Congress of Biochemistry, Lisbon, Portugal, 2002.
Tese / Dissertação
  1. "Regulation of the Expression of Ionotropic Glutamate Receptors by BDNF in Hippocampal Neurons". Doutoramento, 2007. http://hdl.handle.net/10316/9977.

Outros

Outra produção
  1. Supporting Class Tutorials - "Expressão e purificação de proteína recombinante". Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
  2. Supporting Class Tutorials - "Isolamento de DNA plasmídico e análise de restrição". Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
  3. Supporting Class Tutorials - "Clonagem de DNA (Preparação de células competentes e transformação da ligação). Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
  4. Supporting Class Tutorials - "Amplificação de fragmento de DNA por PCR e ligação a um vetor de clonagem". Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
  5. Supporting Class Tutorials - "Visualização de células Hela em mitose: marcação da tubulina e do DNA". Supporting Class Tutorials of Biologia Molecular e Celular that were available at UC-NONIO platform. 2011.
  6. Supporting Class Tutorials - "Variabilidade genética: Estudo da presença da sequência ALU na região PV92 do cromossoma 16". Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.