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Identification

Personal identification

Full name
Margarida Alexandra Vaz Caldeira

Author identifiers

Ciência ID
3016-85A2-C9B5
ORCID iD
0000-0001-5107-1499

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Molecular Biology
Education
Degree Classification
2005 - 2006
Concluded
 Course of Science in Laboratory Animals (Curso de Especialização Tecnológica)
Sociedade Portuguesa de Ciências em Animais de Laboratório, Portugal
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2011 - 2013 Invited Assistant Professor (University Teacher) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2008/02 - 2008/08 Invited Assistant Professor (University Teacher) Universidade de Coimbra - Departamento de Zoologia, Portugal
Universidade de Coimbra - Departamento de Zoologia, Portugal

Others

Category
Host institution 		Employer
2019/01 - Current Doctorate Investigator (DL57) within the project UID/NEU/04539/2013: nº 30 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2015 - 2018 PostDoctoral Fellow - Microscopy Unit (RD Units Strategic Plan: POCI-01-0145-FEDER-007440) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2013 - 2015 PostDoctoral Fellow - Microscopy Unit (CENTRO-07-0224-FEDER-002006: Aging, Stress and Chronic Diseases: From Mechanisms to Therapeutics) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2007/06 - 2011 PostDoctoral Fellow (Mechanisms of regulation of the proteasome activity under excitotoxic conditions)- SFRH/BPD/30432/2006 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Oftalmologia e Ciências da Visão, Portugal
(...)
2002 - 2007 PhD Student Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2001/09/03 - 2002/08/30 Undergraduate Training Student Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
CNS Discovery Research, Janssen Pharmaceutica N. V., a division of Johnson & Johnson Pharmaceutical Research & Development, Belgium
Outputs

Publications

Book
  1. Santos-Carvalho, Ana; Fontes, Adriana; Caldeira, Margarida; Oliveira, Paulo; Ferreira, Sónia. Curso Avançado de Regulação celular, 10º, 11º e 12º anos. Mamarrosa, Portugal: IEC. 2015.
    Published
Book chapter
  1. Carvalho, A. L.; Caldeira, M. V.; Gomes, A. R.; Carvalho, A. P.; Duarte, C. B.. "Regulation of AMPA Receptors by Metabotropic Receptors and Receptor Tyrosine Kinases: Mechanisms and Physiological Roles". In Handbook of Neurochemistry and Molecular Neurobiology, 275-323. Springer US, 2008.
    10.1007/978-0-387-30382-6_12
Conference poster
  1. Cortes, Luisa; Caldeira, Margarida. "Microscope Quality Control". Paper presented in Trends in Microscopy (TiM) Conference, 2025.
  2. Fernandes, Tânia; Caldeira, Margarida. "Impaired ER-mitochondria contacts affect lipid droplets metabolism in an in vitro Alzheimer's disease model". Paper presented in Mitochondria in Aging, Cancer and Cell Death, 2024.
  3. Martins, Gabriel G; Cortes, Luisa; Caldeira, Margarida. "Microscope Quality Control". Paper presented in SPAOM 2024, 2024.
  4. Fernandes, Tânia; Margarida V. Caldeira; Melo, Tânia; Neves, B; Carvalho, C; Resende, Rosa; Filadi, R; et al. "Inhibition of diglyceride acyltransferase (DGAT1) improves the ER-mitochondria interaction in an in vitro Alzheimer's disease model.". Paper presented in 58th Annual Scientific Meeting of the European Society of Clinical (ESCI)., 2024.
  5. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia". Paper presented in EMBO Workshop on Proteostasis: from organelles to organisms, 2019.
  6. Ivan L Salazar; Frisari., S.; Caldeira, Margarida; Duarte, Carlos B.. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemia.". Paper presented in 1st Neuroscience and Disease Retreat from the Center of Neuroscience and Cell Biology, 2019.
  7. "Crosstalk between the Ubiquitin-Proteasome System and calpains in neuronal death induced by brain ischemi". Paper presented in XVII SINS National Congress, Italy, 2017.
  8. "Interplay between the Ubiquitin-Proteasome System and calpains in brain ischemia". Paper presented in ISN-ESN, Paris, France, 2017.
  9. "Brain ischemia-induced alterations in the ubiquitin-proteasome system: role in neuronal demise". Paper presented in 7th ISN Special Neurochemistry Conference- Synaptic Function and Dysfunction In Brain Diseases, Portugal, 2016.
  10. "Excitotoxic glutamate stimulation and oxygen and glucose deprivation downregulate the 26S proteasome in cultured neurons.". Paper presented in 9th FENS Forum of Neuroscience, 2014.
  11. "Glutamate receptor activation is coupled to 26S proteasome downregulation in in vitro ischemia". Paper presented in 5th Conference on Advances in molecular mechanisms underlying neurological disorders, 2013.
  12. Ivan L Salazar; Margarida V. Caldeira; Michele Curcio; Duarte, Carlos B.. "26S Proteasome downregulation under excitotoxic conditions". Paper presented in X CNC Annual Meeting, 2012.
  13. "Excitotoxic stimulation-induced changes in the ubiquitin-proteasome system in neuronal hippocampal cultures". Paper presented in Society for Neuroscience (SfN) 36th Annual Meeting, 2009.
  14. "Changes in the ubiquitin-proteasome system associated with neuronal damage in brain ischemia". Paper presented in XVI National Congress of Biochemistry, 2008.
  15. "BDNF differentially regulates the expression, delivery and activity of AMPA and NMDA receptor subunits in cultured hippocampal neurons". Paper presented in 10th Meeting of the Portuguese Society for Neurosciences, 2007.
  16. "BDNF regulates differentially the expression and delivery of NMDA receptor subunits in cultured hippocampal neurons". Paper presented in XV National Congress of Biochemistry, Aveiro, Portugal, 2006.
  17. "BDNF regulates the expression and traffic of NMDA receptor in cultured hippocampal neurons". Paper presented in Society for Neuroscience (SfN) 36th Annual Meeting, Atlanta, E.U.A., 2006.
  18. "Modulation of AMPA receptors expression and synaptic delivery by BDNF in hippocampal neurons". Paper presented in Society for Neuroscience (SfN) 35th Annual Meeting, Washington, DC, U.S.A., 2005.
  19. "BDNF regulates the expression of AMPA receptor subunits in hippocampal neurons". Paper presented in 9th Meeting of the Portuguese Society for Neurosciences, Luso, Portugal, 2005.
  20. "Regulation of the expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits by brain derived neurotrophic factor in cultured hippocampal neurons". Paper presented in Society for Neuroscience (SfN) 34th Annual Meeting, San Diego, U.S.A., 2004.
  21. "Effect of BDNF on the expression of AMPA receptor subunits in cultured hippocampal neurons.". Paper presented in 4th Forum of European Neuroscience (FENS), Lisbon, Portugal, 2004.
  22. "Novel mGlu1 receptor antagonists are potent antinociceptive agents in animal models involving sensitization of nociception and hyperalgesia: a comparison with reference mGlu1/5 antagonists and morphine". Paper presented in 8th Meeting of the Portuguese Society for Neurosciences, Curia, Portugal, 2003.
  23. "Recently discovered novel mGlu1 receptor antagonists are potent antinociceptive agents in the second phase of the formalin test: a comparison with mGlu1/5 reference antagonists and morphine". Paper presented in XIII National Congress of Biochemistry, Lisbon, Portugal, 2002.
Journal article
  1. Eliane S. Sanches; Ricardo A. Leitão; Filipa I. Baptista; Sandra I. Mota; Margarida V. Caldeira; Paulo J. Oliveira; António F. Ambrósio; Rosa Fernandes; Ana P. Silva. "Methylphenidate triggers retinal oxidative stress and mitochondrial dysfunction under physiological conditions but has beneficial effects in inflammatory settings". Neuropharmacology (2025): https://doi.org/10.1016/j.neuropharm.2025.110623.
    10.1016/j.neuropharm.2025.110623
  2. Pereira AC; Marques AP; Resende R; Serrano-Cuñarro L; Caldeira M; Fernandes T; Batista M; et al. "Changes in the endoplasmic reticulum-mitochondria communication in dermal fibroblasts from early-stage bipolar disorder patients: Skin-brain axis as a new route to understand the pathophysiology of mental illness?". International journal of molecular medicine (2025): http://europepmc.org/abstract/med/41041839.
    10.3892/ijmm.2025.5654
  3. Salazar, Ivan L.; Curcio, Michele; Mele, Miranda; Vetrone, Rossela; Frisari, Simone; Costa, Rui O.; Caldeira, Margarida V.; Trader, Darci J.; Duarte, Carlos B.. "Activation of the 20S proteasome core particle prevents cell death induced by oxygen- and glucose deprivation in cultured cortical neurons". Apoptosis (2025):
    Open access • In press • 10.1007/s10495-025-02097-x
  4. Diana Costa; Carolina Costa; Margarida Caldeira; Luísa Cortes; João A. Queiroz; Carla Cruz. "Targeting of Cellular Organelles by Fluorescent Plasmid DNA Nanoparticles". Biomacromolecules 18 9 (2017): 2928-2936. https://doi.org/10.1021/acs.biomac.7b00877.
    10.1021/acs.biomac.7b00877
  5. Salazar, Ivan; Mele, Miranda; Caldeira, Margarida; Costa, Rui; Correia, Bárbara; Frisari, Simone; Duarte, Carlos. "Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons". BIO-PROTOCOL 7 18 (2017): http://dx.doi.org/10.21769/bioprotoc.2551.
    10.21769/bioprotoc.2551
  6. Caldeira, M.V.; Salazar, I.L.; Curcio, M.; Duarte, C.B.. "The role of proteases in hippocampal synaptic plasticity: Putting together small pieces of a complex puzzle". Neurochemical Research 41 1-2 (2016): 156-182. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946811163&partnerID=MN8TOARS.
    10.1007/s11064-015-1752-5
  7. Caldeira, Margarida; Ferreira, Joana; Carvalho, Ana; Duarte, Carlos. "Biotinylation and Purification of Plasma Membrane-associated Proteins from Rodent Cultured Neurons". BIO-PROTOCOL 6 10 (2016): http://dx.doi.org/10.21769/bioprotoc.1807.
    10.21769/bioprotoc.1807
  8. Salazar IL; Caldeira MV; Curcio M; Duarte CB. "The Role of Proteases in Hippocampal Synaptic Plasticity: Putting Together Small Pieces of a Complex Puzzle.". (2015): http://europepmc.org/abstract/med/26547197.
  9. Caldeira MV; Salazar IL; Curcio M; Canzoniero LM; Duarte CB. "Role of the ubiquitin-proteasome system in brain ischemia: friend or foe?". (2014): http://europepmc.org/abstract/med/24157661.
    10.1016/j.pneurobio.2013.10.003
  10. Caldeira MV; Curcio M; Leal G; Salazar IL; Mele M; Santos AR; Melo CV; et al. "Excitotoxic stimulation downregulates the ubiquitin-proteasome system through activation of NMDA receptors in cultured hippocampal neurons.". (2013): http://europepmc.org/abstract/med/23069389.
    10.1016/j.bbadis.2012.10.009
  11. Baptista MS; Melo CV; Armelão M; Herrmann D; Pimentel DO; Leal G; Caldeira MV; et al. "Role of the proteasome in excitotoxicity-induced cleavage of glutamic acid decarboxylase in cultured hippocampal neurons.". (2010): http://europepmc.org/abstract/med/20405034.
    10.1371/journal.pone.0010139
  12. Santos SD; Carvalho AL; Caldeira MV; Duarte CB. "Regulation of AMPA receptors and synaptic plasticity.". (2009): http://europepmc.org/abstract/med/18424006.
    10.1016/j.neuroscience.2008.02.037
  13. Carvalho AL; Caldeira MV; Santos SD; Duarte CB. "Role of the brain-derived neurotrophic factor at glutamatergic synapses.". (2008): http://europepmc.org/abstract/med/18059328.
    10.1038/sj.bjp.0707509
  14. Caldeira MV; Melo CV; Pereira DB; Carvalho RF; Carvalho AL; Duarte CB. "BDNF regulates the expression and traffic of NMDA receptors in cultured hippocampal neurons.". (2007): http://europepmc.org/abstract/med/17428676.
    10.1016/j.mcn.2007.02.019
  15. Caldeira MV; Melo CV; Pereira DB; Carvalho R; Correia SS; Backos DS; Carvalho AL; Esteban JA; Duarte CB. "Brain-derived neurotrophic factor regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits in hippocampal neurons.". (2007): http://europepmc.org/abstract/med/17337442.
    10.1074/jbc.M700607200
  16. Caldeira, M.V.; Melo, C.V.; Pereira, D.B.; Carvalho, R.; Correia, S.S.; Backos, D.S.; Carvalho, A.L.; Esteban, J.A.; Duarte, C.B.. "Erratum: Brain-derived neurotrophic factor regulates the expression and synaptic delivery of a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits in hippocampal neurons (Journal of Biological Chemistry (2007) 282, (12619-12628))". Journal of Biological Chemistry 282 37 (2007): http://www.scopus.com/inward/record.url?eid=2-s2.0-34848900818&partnerID=MN8TOARS.
Thesis / Dissertation
  1. "Regulation of the Expression of Ionotropic Glutamate Receptors by BDNF in Hippocampal Neurons". PhD, 2007. http://hdl.handle.net/10316/9977.

Other

Other output
  1. Supporting Class Tutorials - "Expressão e purificação de proteína recombinante". Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
  2. Supporting Class Tutorials - "Isolamento de DNA plasmídico e análise de restrição". Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
  3. Supporting Class Tutorials - "Clonagem de DNA (Preparação de células competentes e transformação da ligação). Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
  4. Supporting Class Tutorials - "Amplificação de fragmento de DNA por PCR e ligação a um vetor de clonagem". Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
  5. Supporting Class Tutorials - "Visualização de células Hela em mitose: marcação da tubulina e do DNA". Supporting Class Tutorials of Biologia Molecular e Celular that were available at UC-NONIO platform. 2011.
  6. Supporting Class Tutorials - "Variabilidade genética: Estudo da presença da sequência ALU na região PV92 do cromossoma 16". Supporting Class Tutorials of Laboratórios de Biologia Molecular that were available at UC-NONIO platform. 2011.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2025/02/05 Impact of methylphenidate on oxidative stress and mitochondrial dynamics in retinal cells under control or inflammatory conditions 55th Meeting of the Portuguese Society for Pharmacology (SPF2025)
Portuguese Society for Pharmacology (Porto, Portugal)
2024/06/05 Inhibition of diglyceride acyltransferase (DGAT1) improves the ER-mitochondria interaction in an in vitro Alzheimer´s disease model. 58th Annual Scientific Meeting of the European Society of Clinical (ESCI)
European Society of Clinical (Barcelona, Spain)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2024/05/16 - Current MICRODay - Open day for microscopy (2024/05/16)
Other (Member of the Organising Committee)
 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2024/05/04 - 2024/08/26 Workshop and demonstration of Super-Resolution Microscopy: STEDYCON 2D STED Confocal (2024/05/04)
Workshop (Member of the Organising Committee)
 Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
2024/06/18 - 2024/06/19 Workshop on Zeiss Arivis software (in collaboration with Carl Zeiss Microscopy (2024/06/18 - 2024/06/19)
Workshop (Member of the Organising Committee)
 Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
2024/03 - 2024/03 Brain Awareness Week (2024)
Workshop (Other)
 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2025/03/12 - Current Improving facility user trainings with pedagogy: an interactive CTLS workshop
Workshop
 Core Technologies for Life Sciences (CTLS)
CORE, United Kingdom
2024/06/06 - Current First Aid Training Fist Aid Training; Hiorse - Higiene, Segurança e Saúde no Trabalho
2023/09/22 - Current Green Labs Portugal Symposium: Promoting Sustainability in Research
Symposium
 Green Labs Portugal Symposium: Promoting Sustainability in Research
Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
2022/05/06 - Current Training - Emergency Organisation and Fire Safety (Fire Fighting)
Other
 ¿ Training - Emergency Organisation and Fire Safety (Fire Fighting); Hiorse - Higiene, Segurança e Saúde no Trabalho
Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
2021/03/19 - Current Training - Emergency organisation - Self-protection measures ¿ Training - Emergency organisation - Self-protection measures; Hiorse - Higiene, Segurança e Saúde no Trabalho
2024/11/20 - 2024/11/22 Spanish-Portuguese Meeting for Advanced Optical Microscopy (SPAOM)
Meeting
 SPAOM 2024

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2024/10/17 - 2024/10/18 Pratical Classes in Methodologies in Cellular and Molecular Biology, component of the Master in Molecular and Cellular Biology Methodologies in Cellular and Molecular Biology (Mestrado integrado) Universidade de Coimbra, Portugal
2024/09/26 - 2024/10/03 Pratical Classes in Cellular Regulation, component of the Master in "Investigação Biomédica" (MIB) Master in "Investigação Biomédica" (MIB) (Mestrado integrado) Universidade de Coimbra, Portugal
2024/09/26 - 2024/10/03 Pratical Classes in Cellular Regulation, component of the Master in Molecular and Cellular Biology Master in Molecular and Cellular Biology (Mestrado integrado) Universidade de Coimbra, Portugal
2024/10/02 - 2024/10/02 Pratical Classes in Cellular Regulation, component of the Master in "Investigação Biomédica" (MIB) Cellular Regulation (Mestrado integrado) Universidade de Coimbra, Portugal
2024/05/27 - 2024/05/29 Introduction to QuPath - 3rd Edition (Outros) Portuguese Platform of BioImaging, Portugal