Margarida Candeias Simões (3F1F-C8C9-66E6)

Curriculum Vitae

Margarida Candeias Simões

Margarida Simões (25y) is a PhD student at Protein Misfolding and Amyloids in Biomedicine laboratory, affiliated to BioISI - Biosystems & Integrative Sciences Institute at the Faculty of Sciences of University of Lisbon (FC-ULisboa). Margarida graduated with a BSc in Biochemistry (score 16/20) in 2019 and with a MSc in Medical Biochemistry (score 19/20) in 2022, both at FC-ULisboa. Margarida's academic performance granted her several academic merit awards. As an undergraduate, Margarida gained practical research experience in the field of immunology through a short-term internship at IMM (Instituto de Medicina Molecular). In 2020, she started her MSc research project on "Single-domain antibodies targeting S100B as tools in Alzheimer's disease" at the Protein Misfolding and Amyloids in Biomedicine laboratory at ULisboa under the supervision of Professor Cláudio M. Gomes. Margarida investigated the effects of single-domain antibodies (or nanobodies) targeting S100B as potentiators of S100B anti-aggregation chaperone activity and as modulators of the aggregation of the amyloid-ß peptide and Tau fragment K18. Her MSc thesis defended in January 2022 was graded 20/20 (opponent: Dr. Tiago Bandeiras, Merck Satellite, iBET). After finishing her MSc, Margarida did a 3-month postgraduate Erasmus+ internship at the University of Hohenheim, Stuttgart under the supervision of Professor Guenter Fritz. Currently, Margarida is further exploring her MSc project as a PhD student.

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Identificação

Identificação pessoal

Nome completo: Margarida Candeias Simões
Data de nascimento: 1998/03/23
Género: Feminino

Nomes de citação

Simões, Margarida C.
Margarida C. Simões

Identificadores de autor

Ciência ID: 3F1F-C8C9-66E6
ORCID iD: 0000-0002-6577-3819

Endereços de correio eletrónico

mcsimoes@fc.ul.pt (Profissional)

Domínios de atuação

Ciências Naturais - Ciências Biológicas - Bioquímica

Idiomas

Idioma	Conversação	Leitura	Escrita	Compreensão	Peer-review
Português (Idioma materno)
Inglês	Utilizador proficiente (C1)	Utilizador proficiente (C1)	Utilizador independente (B2)	Utilizador proficiente (C1)

Formação

	Grau	Classificação
2022/09/01 - 2026 Em curso	Biochemistry (specialization in Structural Biochemistry) (Doutoramento) Universidade de Lisboa Faculdade de Ciências, Portugal "Exploiting single-domain antibodies as tools to modulate protein aggregation in neurodegeneration" (TESE/DISSERTAÇÃO)
2019/09 - 2022/01 Concluído	Biochemistry (specialization in Medical Biochemistry) (Mestrado) Universidade de Lisboa Faculdade de Ciências, Portugal "MSc thesis project: "Single-domain antibodies targeting S100B as tools in Alzheimer's disease". Grade: 20/20; Supervisior: Prof. Dr. Cláudio M. Gomes; Discussion: Dr. Tiago Bandeiras (Merck Satellite, iBET)." (TESE/DISSERTAÇÃO)	19
2016/09 - 2019/06 Concluído	Biochemistry (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal	16
2013 - 2016 Concluído	Sciencies and Technology (Ensino secundário) Escola Secundária de Alves Redol, Portugal	16

Percurso profissional

Ciência

	Categoria Profissional Instituição de acolhimento	Empregador
2022/09/01 - 2026	Investigador (Investigação)	Biosystems & Integrative Sciences Institute, Portugal
	PhD research project: "Exploiting single-domain antibodies as tools to modulate protein aggregation in neurodegeneration" (Supervisior: Prof. Dr. Cláudio M. Gomes) at Protein Misfolding and Amyloids in Biomedicine, BioISI, FC-UL , Portugal
2022/04/01 - 2022/06/30	Investigador (Investigação)	Universität Hohenheim, Alemanha
	Postgraduate Erasmus+ internship: "Structural biochemistry of protein: antibody interactions" (Supervisor: Prof. Dr. Guenter Fritz) at University of Hohenheim, Alemanha
2020/09 - 2022/01	Investigador (Investigação)	Biosystems & Integrative Sciences Institute, Portugal
	MSc dissertation project: "Single-domain antibodies targeting S100B as tools in Alzheimer's disease" (Supervisior: Prof. Dr. Cláudio M. Gomes) at Protein Misfolding and Amyloids in Biomedicine laboratory, BioISI, FC-UL, Portugal
2019/03 - 2019/05	Estagiário de Investigação (Investigação)	Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
	Undergraduate internship at Lymphocyte Regulation Laboratory (Supervisor: Prof. Dr. Luís Graça), Instituto de Medicina Molecular (IMM), Portugal

Outras Carreiras

	Categoria Profissional Instituição de acolhimento	Empregador
2022/01/10 - 2022/02/28	Preparador de Análises Clínicas (Técnico de Diagnóstico e Terapêutica - Técnico de Análises Clínicas e de Saúde Pública)	Joaquim Chaves Saúde, Portugal
	Laboratory Technician, Collection and Processing of COVID-19 samples (Part-time), Portugal

Produções

Publicações

Artigo em revista	Simões, Margarida C.; Cristóvão, Joana S.; Pardon, Els; Steyaert, Jan; Fritz, Günter; Gomes, Cláudio M.. "Functional modulation of RAGE activation by multimeric S100B using single-domain antibodies". Journal of Biological Chemistry 300 12 (2024): 107983. https://doi.org/10.1016/j.jbc.2024.107983. Acesso aberto • Aceite para publicação • 10.1016/j.jbc.2024.107983 Justine Mortelecque; Orgeta Zejneli; Séverine Bégard; Margarida C. Simões; Lea El Hajjar; Marine Nguyen; François-Xavier Cantrelle; et al. "A selection and optimization strategy for single-domain antibodies targeting the PHF6 linear peptide within the Tau intrinsically disordered protein". Journal of Biological Chemistry (2024): http://dx.doi.org/10.1016/j.jbc.2024.107163. 10.1016/j.jbc.2024.107163
Poster em conferência	Margarida C. Simões; Joana S. Cristóvão; Els Pardon; Jan Steyaert; Günter Fritz; Cláudio M. Gomes. "Discovery of a library of nanobodies which modulate S100B-mediated RAGE activation". Trabalho apresentado em Inserm Workshop 280: "Exploiting the full potential of NANOBODIES: from research tools to therapeutics, 2024. Margarida C. Simões; Joana S. Cristóvão; Els Pardon; Jan Steyaert; Cláudio M. Gomes. "Identification and characterization of a novel library of single-domain antibodies which compete for RAGE binding and modulate S100B neurotrophic activity". Trabalho apresentado em 4th Chem&Biochem Students Meeting, 2024. Margarida C. Simões; Cláudio M. Gomes. "Heterotypic interactions drive anti-aggregation activity of nanobodies against S100B on Tau aggregation". Trabalho apresentado em 6th Instruct-ERIC Biennial Structural Biology Conference, 2024. Margarida C. Simões; Cláudio M. Gomes. "A novel library of single-domain antibodies capable of modulating Tau aggregation". Trabalho apresentado em AIMS Meeting 2024, 2024. Margarida C. Simões; Cláudio M. Gomes. Autor correspondente: Cláudio M. Gomes. "Heterotypic interactions drive anti-aggregation activity of nanobodies against S100B on Tau aggregation". Trabalho apresentado em ADPD 2024 Advances in Science & Therapy. Internation Conference on Alzheimer's and Parkinson Diseases and related neurological disorders, 2024. Margarida C. Simões; Joana S. Cristóvão; Els Pardon; Jan Steyaert; Cláudio M. Gomes. Autor correspondente: Cláudio M. Gomes. "Nanobodies as protein-based tools to modulate Tau aggregation in Alzheimer's disease". Trabalho apresentado em Ciências Research and Innovation Day 2023, 2023. Margarida C. Simões; Joana S. Cristóvão; Els Pardon; Jan Steyaert; Cláudio M. Gomes. Autor correspondente: Cláudio M. Gomes. "Single-domain antibodies against S100B activate its chaperone function and modulate Tau/K18 aggregation". Trabalho apresentado em FEBS advanced course: Protein folding, aggregation and compartmentalization, 2023. Margarida C. Simões; Joana S. Cristóvão; Els Pardon; Jan Steyaert; Cláudio M. Gomes. Autor correspondente: Cláudio M. Gomes. "Single-domain antibodies as tools for controlling Tau aggregation in neurodegenerative diseases". Trabalho apresentado em 3rd Chem&Biochem Students Meeting, 2023. Margarida C. Simões; Joana S. Cristóvão; Els Pardon; Jan Steyaert; Cláudio M. Gomes. Autor correspondente: Cláudio M. Gomes. "Anti-S100B nanobodies as modulators of Tau aggregation". Trabalho apresentado em EuroTau 2023, 2023. Margarida C. Simões; Joana S. Cristóvão; Els Pardon; Jan Steyaert; Cláudio M. Gomes. Autor correspondente: Cláudio M. Gomes. "Single-domain antibodies targeting S100B as tools in Alzheimer's disease". Trabalho apresentado em TWIN2PIPSA - Twinning for Excellence in Biophysics of Protein Interactions and Self-Assembly, 2023. Margarida C. Simões; Joana S. Cristóvão; Els Pardon; Jan Steyaert; Cláudio M. Gomes. Autor correspondente: Cláudio M. Gomes. "Single-domain antibodies targeting S100B as tools in Alzheimer's disease". Trabalho apresentado em 6º Conferência Anual da RedeSAÚDE (Prémios ULisboa - Rede Saúde 2022), 2022. Joana V. Ribeiro; Guilherme G. Moreira; António J. Figueira; Mariana Romão; Coelho, Romina; Margarida C. Simões; de Freitas, Daniela P.; Cláudio M. Gomes. "Research on Protein Folding Diseases at the Protein Misfolding and Amyloids in Biomedicine Laboratory". Trabalho apresentado em Ciências Research Day 2022, 2022.
Tese / Dissertação	"Single-domain antibodies targeting S100B as tools in Alzheimer's disease". Mestrado, Universidade de Lisboa Faculdade de Ciências, 2022.

Atividades

Apresentação oral de trabalho

	Título da apresentação	Nome do evento Anfitrião (Local do evento)
2024/11/25	Exploiting antibody fragments to inhibit Tau aggregation	6th Students Meeting of the Mind Brain College ULisboa Reitoria da Universidade de Lisboa (Lisbon, Portugal)
2024/06/27	Identification and characterization of a novel library of single-domain antibodies which compete for RAGE binding and modulate S100B neurotrophic activity	4th Chem&Biochem Students Meeting Departamento de Química e Bioquímica, FCUL-ULisboa (Lisbon, Portugal)
2023/09/03	"Single-domain antibodies against S100B activate its chaperone function and modulate Tau/K18 aggregation"	FEBS advanced course: Protein folding, aggregation and compartmentalization FEBS (Spetses Island, Grécia)
2022/11/15	"Single-domain antibodies targeting S100B as tools in Alzheimer's disease"	6º Conferência Anual da RedeSAÚDE (Prémios ULisboa - Rede Saúde 2022) University of Lisbon (Lisbon, Portugal)
2021/02/19	Presentation of the MSc project "Single-domain antibodies targeting S100B as tools in Alzheimer's disease"	3º Encontro do Mestrado em Bioquímica (Virtual) Comissão Pedagógica do Mestrado em Bioquímica (Portugal)

Organização de evento

	Nome do evento Tipo de evento (Tipo de participação)	Instituição / Organização
2023/04 - 2023/07	3rd Chem & Biochem Students Meeting (2023/07/13 - 2023/07/13) Encontro (Membro da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/02/01 - 2023/02/03	TWIN2PISA - Twinning for Excellence in Biophysics of Protein Interaction and Self-Assembly Meeting (2023/02/01 - 2023/02/03) Encontro (Membro da Comissão Organizadora)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

Participação em evento

	Descrição da atividade Tipo de evento	Nome do evento Instituição / Organização
2024/09/25 - 2024/09/26	Inserm Workshop 280: "Exploiting the full potential of NANOBODIES: from research tools to therapeutics" Oficina (workshop)	Inserm Workshop 280: "Exploiting the full potential of NANOBODIES: from research tools to therapeutics" INSERM, França
2024/06/27 - 2024/06/27	4th Chem&Biochem Students Meeting Encontro	4th Chem&Biochem Students Meeting Universidade de Lisboa Departamento de Química e Bioquímica, Portugal
2024/05/23 - 2024/05/24	6th Instruct-ERIC Biennial Structural Biology Conference Conferência	6th Instruct-ERIC Biennial Structural Biology Conference Instruct-ERIC, França
2024/04/16 - 2024/04/19	AIMS Meeting 2024 Encontro	AIMS Meeting 2024 Universidade de Lisboa, Portugal
2024/03/05 - 2024/03/09	ADPD 2024: Advances in Science & Therapy - International Conference on Alzheimer's and Parkinson's Disease and related neurologic disorders Conferência	ADPD 2024: Advances in Science & Therapy - International Conference on Alzheimer's and Parkinson's Disease and related neurologic disorders
2024/02/07 - 2024/02/07	Online Workshop: Tagging endogenous proteins with CRISPR/Cas9 to study protein-protein interactions Oficina (workshop)	Online Workshop: Tagging endogenous proteins with CRISPR/Cas9 to study protein-protein interactions Twinning for Excellence in Biophysics of Protein Interactions and Protein Self-Assembly (TWIN2PIPSA), Portugal
2024/02/01 - 2024/02/02	UCPH On-site training: Computational tools to analyse protein stability and abundance, and protein variant effects Oficina (workshop)	UCPH On-site training: Computational tools to analyse protein stability and abundance, and protein variant effects Twinning for Excellence in Biophysics of Protein Interactions and Protein Self-Assembly (TWIN2PIPSA), Portugal
2023/11/13 - 2023/11/15	TWIN2PIPSA Training School - Integrating experimental and computational approaches in protein self-assembly, Copenhagen Oficina (workshop)	TWIN2PIPSA Training School - Integrating experimental and computational approaches in protein self-assembly Københavns Universitet - Nørre Campus, Dinamarca Twinning for Excellence in Biophysics of Protein Interactions and Protein Self-Assembly (TWIN2PIPSA), Portugal
2023/10/24 - 2023/10/24	Ciências Research and Innovation Day	Ciências Research and Innovation Day 2023 Universidade de Lisboa Faculdade de Ciências, Portugal
2023/09/01 - 2023/09/08	FEBS advanced course: Protein Folding, Aggregation and Compartmentalization Outro	FEBS advanced course: Protein Folding, Aggregation and Compartmentalization Federation of European Biochemical Societies, Reino Unido
2023/07/13 - 2023/07/13	3rd Chem&Biochem Students Meeting Encontro	3rd Chem&Biochem Students Meeting Universidade de Lisboa Faculdade de Ciências, Portugal
2023/04/27 - 2023/04/28	EuroTau 2023 Conferência	EuroTau 2023 Université de Lille, França
2023/02/01 - 2023/02/03	TWIN2PIPSA - Twinning for Excellence in Biophysics of Protein Interactions and Self-Assembly Plenary Conference Series Conferência	TWIN2PIPSA - Twinning for Excellence in Biophysics of Protein Interactions and Self-Assembly Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2022/11/13 - 2022/11/16	Basics in Light Microscopy course Outro	Basics in Light Microscopy course Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2022/11/15 - 2022/11/15	6º Conferência Anual da RedeSAÚDE: Perspetivas Multidisciplinares e Saúde Global na Lisboa Conferência	6º Conferência Anual da RedeSAÚDE: Perspetivas Multidisciplinares e Saúde Global na Lisboa Universidade de Lisboa, Portugal
2022/09/26 - 2022/09/26	Ciências Research Day Conferência	Ciências Research Day 2022 Universidade de Lisboa Faculdade de Ciências, Portugal
2022/03/23 - 2022/03/23	The Protein Society Webinar Series: Protein Folding and Misfolding on the Ribosome Seminário	Protein Folding and Misfolding on the Ribosome Indiana University, Estados Unidos
2022/03/11 - 2022/03/11	Portuguese Centre for Integrated Structural Biology (PCISBIO) Day Seminário	PCISBIO Day PCISBIO - Portuguese Centre for Integrated Structural Biology, Portugal
2022/03/09 - 2022/03/09	Brain Awareness Week: Understanding the Cellular Roots of Alzheimer's disease (Webinar) Conferência	Brain Awareness Week Allen Institute for Brain Science, Estados Unidos
2022/02/22 - 2022/02/23	6th Ulm Meeting - Biophysics of Amyloid Formation (Virtual Meeting) Encontro	Biophysics of Amyloid Formation Universität Ulm, Alemanha
2021/01 - 2021/09	Weekly Seminars from Proteostasis Consortium Seminars Series Seminário	Proteostasis Consortium Seminars Northwestern University, Estados Unidos
2021/05/24 - 2021/05/27	Instruct-ERIC course in Biophysics, Structural Mass Spectrometry and X-ray techniques	Instruct-ERIC course in Biophysics, Structural Mass Spectrometry and X-ray techniques Instruct-ERIC, França

Curso / Disciplina lecionado

	Disciplina	Curso (Tipo)	Instituição / Organização
2022/10/28 - Atual	Teaching Assistant in the subject Projeto Experimental I (Protein Structural Biochemistry module) of the FC-UL MSc programme in Biochemistry and Biomedicine	Projeto Experimental I (Mestrado)	Universidade de Lisboa Faculdade de Ciências, Portugal

Expedição científica

	Descrição da atividade	Instituição / Organização
2024/11/11 - 2024/11/15	Short-term exchange (1 week) to conduct NMR studies under the scope of the PhD studies at CNRS, University of Lille, INSERM, Institute Pasteur de Lille, France. Supervision by Dr. Isabelle Landrieu - Structural mapping of the interactions between nanobodies and Tau fragments	Université de Lille, França
2023/11/27 - 2023/12/02	Short-term exchange (1 week) to conduct NMR studies under the scope of the PhD studies at CNRS, University of Lille, INSERM, Institute Pasteur de Lille, France. Supervision by Dr. Isabelle Landrieu - Structural mapping of the interactions between nanobodies and Tau	Université de Lille, França
2022/04/01 - 2022/06/30	Postgraduate Erasmus+ internship (3 months) to conduct X-ray crystallography studies of protein complexes at the University of Hohenheim, Germany. Supervision by Dr. Guenter Fritz - Structural biochemistry of protein:antibody interactions.	Institute of Biology of the University of Hohenheim, Alemanha

Membro de associação

	Nome da associação	Tipo de participação
2022/01/20 - Atual	Sociedade Portuguesa de Bioquímica - Portuguese Biochemical Society	Member
2022/01/20 - Atual	Sociedade Portuguesa de Biofísica - Portuguese Biophysics Society	Member

Tutoria

	Tópico	Nome do aluno
2023/10/20 - Atual	Expression and purification of recombinant proteins	Inês Carolino
2023/09/11 - 2023/10/03	Kinetic analysis of the effects of anti-tau antibodies on tau aggregation	Lea El hajjar
2022/10/01 - 2022/10/30	Protein-protein interaction studies	Ilse van Dam

Distinções

Prémio

2024	Best Flash Pitch Award in Biochemistry at the 4th Chem&Biochem Students Meeting Universidade de Lisboa Departamento de Química e Bioquímica, Portugal
2023	FEBS Youth Travel Fund to attend FEBS 2023 advanced course: Protein folding, aggregation and compartmentalization Federation of the European Biochemical Societies, Reino Unido
2022	FCT Doctoral Fellowship (2022.11023.BD) Fundação para a Ciência e a Tecnologia, Portugal
2022	Best Master's Award in the panel of Ageing, Cardiology and Neurodegeneration Diseases Prémios ULisboa - redeSAÚDE 2022, Portugal Universidade de Lisboa, Portugal
2020	FC-UL Academic Merit Award (3th year classification: 16,20/20) Universidade de Lisboa Faculdade de Ciências, Portugal
2019	FC-UL Academic Merit Award (2nd year classification: 16,20/20) Universidade de Lisboa Faculdade de Ciências, Portugal
2018	FC-UL Academic Merit Award (1st year classification: 15,94/20) Universidade de Lisboa Faculdade de Ciências, Portugal