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After my PhD, I worked on the project ComplexiTE (Reis, Advanced ERC grant 321266) specifically in the fabrication of gradients with microfluidics. In this time we develop a new technique to encapsulate cells in 3D microenvironments along the fibre. We also develop a method to acquire the entire fibre at the microscope so that image analysis techniques can be used to analyse the fluorescent signal coming from cells and as such their response to that particular combination of materials. This technique was new and allowed a high throughput screening of cell-material interaction and we decided to patent it (WO2018078562A1). During this time I also participated in the Microliver project (Bishi, PTDC/BBB-ECT/4317/2014) where the aforementioned technique was used to obtain Janus particles (particles with two distinct sections) with di¿erent cells entrapped in the sections. We were able to obtain Janus particles without using emulsions and using mild processing conditions that don't harm cells. The relative amount of the sections forming the particle can be easily controlled to screen the response of cells in di¿eretn co-culture conditions. Furthermore, I'm involved in two other projects (Marques, ERC ECM_INK 726061, Dressing4Scars M-ERA-NET2/0013/2016.) which are focused on developing techniques and methods for the regeneration of the skin. I have supervised the activity of 5 PhD students and 1 undergraduate. In the last 5 years, I have authored 12 publications (699 citations from 2016 – Google scholar, 10.8 average impact factor), 1 book chapter and 4 patents at different legal stages with an international spotlight for the Nature Materials Review, a work highlighted at the 5th anniversary of the journal and now in the top 0.1 % of the field of materials engineering (web of science). I am also a team member of two proof of concepts grants (ERC-2019-PoC-899953, ERC-2019-PoC-862595) In my curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: Chondroitin sulfate; Fiber-based techniques; Hyaluronic acid; Microfluidics; Polyelectrolyte complexation; Tendon; microfludics; Science & Technology; Gellan gum; Octadecylamine; Physical hydrogels; Thermal scanning rheology; Mechanical properties; Growth factor immobilization; Hydrogel; Cell encapsulation; Hydrogels; Natural polymers; Microencapsulation; Adipose-derived stem cells; Hydrogel;; Injectable; Methacrylated gellan gum; Osteogenic differentiation; Bioprinting; Cell viability; Cell-laden hydrogels; Osmotic regulator; Sucrose; Biomaterials; Polymers; Microfibers; Microfluidic technique; Drug delivery; Hydrogel fibers; .
Identificação

Identificação pessoal

Nome completo
Luca Gasperini

Nomes de citação

  • Gasperini, Luca
  • Gasperini, L.
  • Luca Gasperini
  • L. Gasperini
  • L Gasperini

Identificadores de autor

Ciência ID
1310-03A5-C495
ORCID iD
0000-0003-2133-4379
Google Scholar ID
TAbAmyAAAAAJ

Domínios de atuação

  • Ciências da Engenharia e Tecnologias - Engenharia dos Materiais

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Italiano (Idioma materno)
Inglês Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Português Utilizador elementar (A1) Utilizador independente (B1) Utilizador elementar (A1) Utilizador elementar (A1)
Formação
Grau Classificação
2013/04/15
Concluído
Biomaterials (Dottorato di Ricerca)
Università degli Studi di Trento, Itália
"Strategies for cells encapsulation and deposition" (TESE/DISSERTAÇÃO)
Excellent
2009/10/26
Concluído
Materials Engineering (Laurea)
Especialização em Biomaterials
Università degli Studi di Trento, Itália
"Strategies for neural regeneration" (TESE/DISSERTAÇÃO)
Percurso profissional

Ciência

2013/11/01 - Atual Pós-doutorado (Investigação)
Empregador
Biomaterials Biodegradables and Biomimetics, Portugal
2013/11/01 - Atual Pós-doutorado (Investigação)
Empregador
Laboratório Associado ICVS 3B's, Portugal
Projetos

Bolsa

Designação Financiadores
2020 - Atual BioPrint4All - A 3D-bioprinting platform with exchangeable tailor-made print heads to engineer tissue surrogates
ERC-2019-PoC-899953
Bolseiro de Pós-Doutoramento
2021 - 2025/08/30 ECaBox "Eyes in a Care Box": Regenerating human retina from resuscitated cadaveric eyes
info:eu-repo/grantAgreement/EC/H2020/964342/EU
Bolseiro de Investigação
European Commission
2020 - 2021/06/30 PATCHED - Microfabricated hydrogel-based complex patch with time dependent controlled multiple-release of biomolecules for improved healing of skin Wounds
ERC-2019-PoC-862595
Bolseiro de Pós-Doutoramento
European Research Council
2013 - 2018/04/01 ComplexiTE - An integrated multidisciplinary tissue engineering approach combining novel high-throughput screening and advanced methodologies to create complex biomaterials-stem cells constructs
ERC-2012-ADG 20120216-321266
Bolseiro de Pós-Doutoramento
Biomaterials Biodegradables and Biomimetics, Portugal
European Research Council

Projeto

Designação Financiadores
2018 - 2021/12/31 Automated high-content screening of YAP/TAZ as intracellular mechanosensing rheostat and therapeutic target in a 3D hepatocellular carcinoma microspheroid model for pre-clinical drug development
PTDC/BTM-ORG/31564/2017
Universidade do Minho, Portugal
Fundação para a Ciência e a Tecnologia
2018 - 2019/01/31 ECM-INK - Cells-self Extracellular Matrices-based Bioinks to create accurate 3D diseased skin tissue models
ERC-2016- COG-726061
Bolseiro de Pós-Doutoramento
Biomaterials Biodegradables and Biomimetics, Portugal
European Research Council

Outro

Designação Financiadores
2017 - Atual Automated high-content screening of YAP/TAZ as intracellular mechanosensing rheostat and therapeutic target in a 3D hepatocellular carcinoma microspheroid model for pre-clinical drug development
PTDC/BTM-ORG/31564/2017
Biomaterials Biodegradables and Biomimetics, Portugal
2017 - Atual Dressing4Scars - New 4D printing dressing to treat skin scars
M-ERA.NET/0013/ 2016
Bolseiro de Pós-Doutoramento
Fundação para a Ciência e a Tecnologia
2014 - 2019/05/31 MicroLiver - A novel bi-compartmentalized hydrogel microcapsule as 3D co-culture system for liver tissue microengineering and drug-induced hepatotoxicity screening
P2020-PTDC/BBB-ECT/4317/2014
Bolseiro de Pós-Doutoramento
Biomaterials Biodegradables and Biomimetics, Portugal
Associação para a Inovação e Desenvolvimento da FCT
2015 - 2018/11/30 GENE2SKIN - Roadmap for advanced genetic engineering-based skin therapies,
692221
Investigador Pós-doutorado
Produções

Publicações

Artigo em revista
  1. Carlos F. Guimarães; Luca Gasperini; Alexandra P. Marques; Rui L. Reis. "3D flow-focusing microfluidic biofabrication: One-chip-fits-all hydrogel fiber architectures". Applied Materials Today 23 (2021): 101013-101013. https://doi.org/10.1016%2Fj.apmt.2021.101013.
    10.1016/j.apmt.2021.101013
  2. Carla M. Abreu; Luca Gasperini; Manuela E. L. Lago; Rui L. Reis; Alexandra P. Marques. "Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages". Bioengineering & Translational Medicine (2020): https://doi.org/10.1002/btm2.10195.
    10.1002/btm2.10195
  3. Canadas, Raphaël F.; Patrício, Pedro; Brancato, Virginia; Gasperini, Luca; Caballero, David; Pires, Ricardo A.; Costa, João B.; et al. "Convection patterns gradients of non-living and living micro-entities in hydrogels". Applied Materials Today 21 (2020): 100859. http://dx.doi.org/10.1016/j.apmt.2020.100859.
    Publicado • 10.1016/j.apmt.2020.100859
  4. Carla M. Abreu; Mariana T. Cerqueira; Rogério P. Pirraco; Luca Gasperini; Rui L. Reis; Alexandra P. Marques. "Rescuing key native traits in cultured dermal papilla cells for human hair regeneration". Journal of Advanced Research (2020): https://doi.org/10.1016/j.jare.2020.10.006.
    10.1016/j.jare.2020.10.006
  5. Carlos F. Guimarães; Luca Gasperini; Alexandra P. Marques; Rui L. Reis. "The stiffness of living tissues and its implications for tissue engineering". Nature Reviews Materials (2020): https://doi.org/10.1038/s41578-019-0169-1.
    10.1038/s41578-019-0169-1
  6. Carlos F. Guimarães; Luca Gasperini; Raquel S. Ribeiro; Andreia F. Carvalho; Alexandra P. Marques; Rui L. Reis. "High-throughput fabrication of cell-laden 3D biomaterial gradients". Materials Horizons (2020): https://doi.org/10.1039/D0MH00818D.
    10.1039/D0MH00818D
  7. Stefano Agnello; Luca Gasperini; João F. Mano; Giovanna Pitarresi; Fabio S. Palumbo; Rui L. Reis; Gaetano Giammona. "Synthesis, mechanical and thermal rheological properties of new gellan gum derivatives". International Journal of Biological Macromolecules 98 (2017): 646-653. https://doi.org/10.1016%2Fj.ijbiomac.2017.02.029.
    10.1016/j.ijbiomac.2017.02.029
  8. A F Carvalho; L Gasperini; R S Ribeiro; A P Marques; R L Reis. "Control of osmotic pressure to improve cell viability in cell-laden tissue engineering constructs". Journal of Tissue Engineering and Regenerative Medicine (2017): https://doi.org/10.1002%2Fterm.2432.
    10.1002/term.2432
  9. Patuzzo, S.; Goracci, G.; Ciliberti, R.; Gasperini, L.. "3D Bioprinting Technology: Scientific Aspects and Ethical Issues". Science and Engineering Ethics (2017): 1-14. http://www.scopus.com/inward/record.url?eid=2-s2.0-85025131235&partnerID=MN8TOARS.
    10.1007/s11948-017-9918-y
  10. Raquel Costa-Almeida; Luca Gasperini; João Borges; Pedro S. Babo; Márcia T. Rodrigues; João F. Mano; Rui L. Reis; Manuela E. Gomes. "Microengineered Multicomponent Hydrogel Fibers: Combining Polyelectrolyte Complexation and Microfluidics". ACS Biomaterials Science & Engineering (2016): https://doi.org/10.1021%2Facsbiomaterials.6b00331.
    10.1021/acsbiomaterials.6b00331
  11. Stefano Agnello; Luca Gasperini; Rui L. Reis; João F. Mano; Giovanna Pitarresi; Fabio S. Palumbo; Gaetano Giammona. "Microfluidic production of hyaluronic acid derivative microfibers to control drug release". Materials Letters (2016): http://dx.doi.org/10.1016/j.matlet.2016.07.014.
    10.1016/j.matlet.2016.07.014
  12. Mariana B. Oliveira; Catarina A. Custódio; Luca Gasperini; Rui L. Reis; João F. Mano. "Autonomous osteogenic differentiation of hASCs encapsulated in methacrylated gellan-gum hydrogels". Acta Biomaterialia (2016): http://dx.doi.org/10.1016/j.actbio.2016.05.033.
    10.1016/j.actbio.2016.05.033
  13. Luca Gasperini; Devid Maniglio; Antonella Motta; Claudio Migliaresi. "An Electrohydrodynamic Bioprinter for Alginate Hydrogels Containing Living Cells". Tissue Engineering Part C: Methods 21 2 (2015): 123-132. http://dx.doi.org/10.1089/ten.tec.2014.0149.
    10.1089/ten.tec.2014.0149
  14. Volha Liaudanskaya; Luca Gasperini; Devid Maniglio; Antonella Motta; Claudio Migliaresi. "Assessing the Impact of Electrohydrodynamic Jetting on Encapsulated Cell Viability, Proliferation, and Ability to Self-Assemble in Three-Dimensional Structures". Tissue Engineering Part C: Methods (2014): 150127064141009-150127064141009. http://dx.doi.org/10.1089/ten.tec.2014.0228.
    10.1089/ten.tec.2014.0228
  15. L. Gasperini; J. F. Mano; R. L. Reis. "Natural polymers for the microencapsulation of cells". Journal of The Royal Society Interface 11 100 (2014): 20140817-20140817. http://dx.doi.org/10.1098/rsif.2014.0817.
    10.1098/rsif.2014.0817
  16. L. Gasperini; D. Maniglio; C. Migliaresi. "Microencapsulation of cells in alginate through an electrohydrodynamic process". Journal of Bioactive and Compatible Polymers 28 5 (2013): 413-425. http://dx.doi.org/10.1177/0883911513501599.
    10.1177/0883911513501599
Capítulo de livro
  1. Gasperini, Luca. "Microfluidics for Processing of Biomaterials.". 2020.
    10.1007/978-3-030-36588-2_2
Poster em conferência
  1. Reis Daniel; Fidalgo, Càtia; Gasperini, Luca; Rui L. Reis; Marques, A. P.. "Bio-tailored gellan gum-based bioinks". Trabalho apresentado em Termis Europe workshop 2019, 2019.
  2. Gasperini, Luca; Carvalho, A. F.; Ribeiro, R. S.; Marques, A. P.; Reis, R. L.. "Multicomponent hydrogel patches for wound healing". Trabalho apresentado em GeneSkin 2018, 2018.
  3. Carvalho, A. F.; Ribeiro, R. S.; Gasperini, Luca; Silva, L.P; Marques, A. P.; Reis, R. L.. "High-throughput screening of optimal osteogenic differentiation conditions in Gellan Gum hydrogel microfibers". Trabalho apresentado em ESB 2018, 2018.
  4. Gasperini, Luca; Carvalho, A. F.; Ribeiro, R. S.; Marques, A. P.; Reis, R. L.. "Microfilm-inspired high-throughput platform to screen 3D cell-materials interactions". Trabalho apresentado em Termis World 2018, 2018.
  5. Gasperini, Luca; Carvalho, A. F.; Ribeiro, R. S.; Marques, A. P.; Reis, R. L.. "Segmented fibers-based high-throughput platform to screen 3D cellmaterials interactions". Trabalho apresentado em TermStem 2018, 2018.
  6. Gasperini, Luca; Carvalho, A. F.; Ribeiro, R. S.; Marques, A. P.; Reis, R. L.. "Microfluidic oil-free fabrication of multiple 3D platforms to screen cell-material interactions". Trabalho apresentado em Termstem 2017, 2017.
  7. Agnello, Stefano; Gasperini, Luca; Mano, J. F.; Pitarresi, Giovanna; Palumbo, Fabio S.; Giammona, Gaetano; Reis, R. L.. "Hyaluronic acid and gellan gum derivatives for tissue engineering and drug release". Trabalho apresentado em TermStem 2016, 2016.
  8. Agnello, Stefano; Gasperini, Luca; Mano, J. F.; Pitarresi, Giovanna; Palumbo, Fabio S.; Giammona, Gaetano; Reis, Rui L.. "Production of hyaluronic acid derivative microfibers with controlled dimension as potential drug delivery system". Trabalho apresentado em Biotecnologie: Ricerca di Base, Interdisciplinare e Traslazionale in Ambito Biomedico, 2015.
  9. Gasperini, Luca; Claudio Migliaresi; Devid Maniglio. "Electro Hydro Dynamic (ehd) Encapsulation of Cells in Alginate Based Hydrogels". Trabalho apresentado em XXXVIII Congress of the European Society for Artificial Organs, 2011.
    10.1111/j.1525-1594.2011.01336.x
Resumo em conferência
  1. Gasperini, Luca; Ribeiro, R. S.; Carvalho, Andreia; Marques, A. P.; Reis, R. L.. "Gellan gum rheological properties and bioprinting". Trabalho apresentado em Termis Europe workshop 2019, Nantes, 2019.
    Publicado
  2. Gasperini, Luca; Carvalho, A. F.; Ribeiro, R. S.; Marques, A. P.; Reis, R. L.. "Segmented microfibers as high-throughput platform to screen 3D cell-materials interactions". Trabalho apresentado em Termis Europe 2019, Rhodes, 2019.
    Aceite para publicação
  3. Guimarães, C. F.; Gasperini, Luca; Ribeiro, R. S.; Pinto, A. C.; Carvalho, A. F.; Marques, A. P.; Reis, R. L.. "Microfluidic fabrication of 3D biomaterial libraries for high-throughput screening stem cell responses". Trabalho apresentado em Termis Europe 2019, Rhodes, 2019.
    Publicado
  4. Guimarães, Carlos; Gasperini, Luca; Ribeiro, R. S.; Carvalho, A. F.; Marques, A. P.; Reis, R. L.. "From low-to high-throughput: Microfluidic fabrication of novel 3D biomaterial libraries for screening cell responses". Trabalho apresentado em ESB 2018, Maastrich, 2018.
    Aceite para publicação
  5. Gasperini, Luca; Carvalho, A. F.; Ribeiro, R. S.; Marques, A. P.; Reis, R. L.. "Microfluidic oil-free fabrication of multiple 3D platforms to screen cell-material interactions". Trabalho apresentado em Termis Europe, Davos, 2017.
    Publicado
  6. DK Bishi; Gasperini, Luca; Marques, A. P.; Mano, J. F.; Reis, R. L.. "Development of Janus hydrogel microcapsules as novel biomaterials-stem cells construct for 3D co-culture applications". Trabalho apresentado em Termis World, Boston, 2015.
    Publicado • 10.1089/ten.tea.2015.5000.abstracts
  7. Agnello, Stefano; Gasperini, Luca; Reis, R. L.; Mano, J. F.; Pitarresi, Giovanna; Palumbo, Fabio S.; Giammona, Gaetano. "Production of hyaluronic acid derivative microfibers with controlled dimension as potential drug delivery system". Trabalho apresentado em Bio Tecnologie Ricerca di base interdisciplinare traslazione in ambito biomedico, 2015.
  8. Gasperini, Luca; Mano, J. F.; Reis, R. L.. "A microfluidic strategy for oil-free cell encapsulation". Trabalho apresentado em TermStem 2015, 2015.
  9. Gasperini, Luca; Mano, J. F.; Reis, R. L.. "High-throughput microencapsulation of cells in microparticles formulated using mixtures of distinct natural-based macromolecules". 2014.
  10. Volha Liaudanskaya; Gasperini, Luca; Antonella Motta; Claudio Migliaresi. "Organ printing and stem cells as future of regenerative medicine: influence of the cell encapsulation system on cell behavior.". Trabalho apresentado em TermStem 2012, Guimarães, 2012.
    Publicado • 10.1002/term.1608

Propriedade Intelectual

Patente
  1. Gasperini, Luca. 2018. "Fibers With Segments, Their Preparation And Applications Thereof".
Pedido provisório de patente
  1. 2020. "BIOREACTOR FOR TISSUE ENGINEERING OF MULTI-TISSUE STRUCTURE AND MANUFACTURING METHOD THEREOF".
    Divulgado
  2. 2020. "MULTICOMPARTEMENT HYDROGEL FIBRE THEIR PREPARATION AND USES THEREOF".
    Divulgado
  3. 2019. "HYDROGEL-LIKE PARTICLES, METHODS ANS USES THEREOF".
    Divulgado
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2019/08 Rheological properties of gellan gum for bioprinting Termis-EU Workshop 3D Printing in Cancer Research
Termis (Nantes, França)
2019/05 Microfluidic fabrication of 3D biomaterial libraries for high-throughput screening stem cell responses Termis European Chapter Meeting
Termis (Rhodes, Grécia)
2019/05 Segmented microfibers as a high-throughput platform to screen 3D cell-materials interactions Termis European Chapter Meeting
Termis (Rhodes, Grécia)
2018/09 From low- to high-throughput: Microfluidic fabrication of novel 3D biomaterial libraries for screening cell responses 29th European Conference on Biomaterials
Europeans Society Biomaterials (Maastricht, Países Baixos)
2018/08 High-throughput screening of optimal osteogenic differentiation conditions 29th European Conference on Biomaterials
Europeans Society Biomaterials (Maastricht, Países Baixos)
2018/06 From low to high-throughput: Development of a 3D Gellan Gum gradient to screen the effect of hydrogel mechanics on ASC commitment Chem2Nature Summer School on Multifunctional biomedical devices
3Bs (Porto, Portugal)
2017/06 Rheological properties of gellan gum for biomedical applications Chem2Nature Second School
3Bs (Porto, Portugal)
2017/06 Microfluidic oil-free fabrication of multiple 3D platforms to screen cell-material interactions Termis European Chapter Meeting
Termis (Davos, Suiça)
2016/11 Screening of 3D cell-materials interactions: A Microfluidic approach Chem2Nature First School
3Bs (Guimarães, Portugal)
2016/11 Development of Janus droplet generation device for hepatocyte-endothelial cell co-culture applictions Chem2Nature First School
3Bs (Guimarães, Portugal)
2016/10 A Microfluidic-Based Platform for the Screening of 3D Cell-Biomaterials Interactions TermStem
3Bs (Guimarães, Portugal)
2016 Exploring amphiphilic derivatives of carbohydrate polymers for biomedical applications Chem2Nature
3B's (Portugal)
2015/06 A microfluidic strategy for oil-free cell encapsulation Polaris
3Bs (Guimarães, Portugal)
2014/10 High-throughput microencapsulation of cells in microparticles formulated using mixtures of distinct natural-based macromolecules TermStem
3Bs (Porto, Portugal)
2014/10 Three dimensional co-cultures in janus-like hydrogel microcapsules TermStem
3Bs (Porto, Portugal)

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2021/02/15 - Atual Bioprinted models to study cancer invasiveness
Coorientador
2018/09 - Atual Establishing a Dynamic In Vitro System to Enhance the Functionality of 3D-Bioprinted Skin-like Constructs
Coorientador
Biomaterials Biodegradables and Biomimetics, Portugal
2017/09/05 - Atual High-Throughput Fabrication, Screening and Sorting of 3D Cell-Biomaterial Constructs towards Combinatorial Tissue Engineering.
Coorientador
Biomaterials (Doutoramento)
Biomaterials Biodegradables and Biomimetics, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2016/10/24 - 2018/10/26 Organization of 5 Gene2skin conferences and schools (talks, posters and activities) (2016/10/24 - 2018/10/26)
Conferência (Membro da Comissão Organizadora)
Biomaterials Biodegradables and Biomimetics, Portugal

Consultoria / Parecer

Descrição da atividade Instituição / Organização
2017/08/30 - Atual Development of a bioprinter for the ECM_INK project in collaboration with BeeVeryCreative. BeeVeryCreative had experience in 3D printers for rapid prototyping and my role was to help in the design of the necessary modification to transform a 3D printer into a bioprinter. My duty included the design of the extruders and the adaption of the 3d printer to work in sterile conditions. BEEVC Electronic Systems Lda, Portugal

Entrevista (jornal / revista)

Descrição da atividade Jornal / Forum
2019/06/17 Patched press release Dignus
2019/06/16 Patched press release Jornal Economico
2019/06/09 Patched press release Sul Informacao
2019/06/07 Patched press release Ver Portugal
2019/05/27 Patched press release Mais Guimaraes
2019/05/17 Patched press release Reflexo digital

Membro de associação

Nome da associação Tipo de participação
2020 - Atual ESB - The European Society for Biomaterials
2014 - Atual Termis European chapter N: 3013

Tutoria

Tópico Nome do aluno
2015/03 - 2015/11 Characterization of new carbohydrate derivatives Stefano Agnello
2012/01 - 2013/09 Assessing the Impact of Electrohydrodynamic Jetting on Encapsulated Cell Viability, Proliferation, and Ability to Self-Assemble in Three-Dimensional Structures. Volha Liaudanskaya
2012/11 - 2013/03 Bioprinting Silvia Lucchi