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Passionate researcher with 10 years of post-PhD experience in diverse area of plant molecular biology, Vitiviniculture, plant physiology, Plant pathology, genetic engineering and modern genome editing technology, tissue culture, biochemistry, nanotechnology, biocontrol agents development and formulations, transcriptomics, proteomics, metabolomics. The research profile includes 1745 citations, H index-20 and 94 peer reviewed published scientific communications including 1 granted patent and 1 provisional submission recently. I gained academic and industrial research experience while working in national and international institutes and industries of good repute. I did my first postdoc project (September 2013- January 2016) at department of Biology, University of Minho, Braga, Portugal where I studied the recalcitrance nature of Hypericum perfoliatum and the complex molecular mechanism with its interaction to Agrobacterium tumefaciens. During this tenure, I had an opportunity to supervise a team of 3 young researchers (one PhD and 2 master students) where I was responsible to design the experimental setup and ensuring the high value results. I started my second postdoc (July 2016-March 2020) in Department of Agronomy, Vitiviniculture lab, University of Tras-O-Montes and Alto Douro, Vila Real, Portugal. My studies were based on grapevines during this project; which brought me plenty of experience in metabolomics pathway engineering, CRISPR mediated genome editing in grapevine, team leading, industrial experience and communication with stake holders. During this tenure, I have been working as a senior member in the lab and management of one research line in the INNOVINE&WINE Innovation platform of Vineyard and Wine (cofinanced by European Regional Development Fund, FEDER, with a total budget of 5 293 984,76 ) project. I have been leading a team of 7 peoples (supervise 2 MSc) for performing large scale field trials on different vineyards in Douro region. The research was performed in collaboration with academia and industries and the major outcome during this work was chitosan based nanomaterial preparation and application in vineyards. The findings were promising which was patented nationally and for European Union territories; while several good articles have been published. Additionally; my research has been ranked second for ENOFORUM AWARD in The Spanish edition of Enoforum 2020 among 35 scientific works in the wine sector from 21 research groups of different countries. After 2 postdoc projects, I moved to Ireland to gain experience in industrial research and started working as Principal Investigator in MBIO (Bio-control group, Monaghan Biosciences, Monaghan county, Ireland). Major duties during this tenure were leading the team of scientists, technicians and research trainees, Project management, establishment of new research facilities, Collaborations with universities. I have been also responsible for scale-up process development, validation of biocontrol/bio stimulants/plant growth promoting biological products and product commercialization. Later I joined as PhD researcher (January 2022-December 2022) in InnovPlantProtect Collaborative Laboratory, Department of Protection of Specific Crops, Elvas, Portugal where 1 provisional patent have been submitted within a year of my tenure. Currently i am working as Researcher biologist in UTAD. I did my PhD from Plant Molecular Biology and Genetic Engineering Department, Defense Institute of Bioenergy Research, India. Recently I have been invited as an external expert by National Research and Development Agency (ANID), Government of Chile for evaluation of research proposals for national funding. Additionally i have been interviewed by french magazine regarding my recent research in Grapevine as a new and sustainable practice in vitiviniculture.
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Identificação

Identificação pessoal

Nome completo
Rupesh Kumar Singh
Género
Masculino

Nomes de citação

  • Singh, Rupesh

Identificadores de autor

Ciência ID
0E1F-ED9C-4F23
ORCID iD
0000-0002-2536-1967
Google Scholar ID
https://scholar.google.com/citations?user=vPIqviYAAAAJ&hl=en

Endereços de correio eletrónico

  • rupeshbio702@gmail.com (Pessoal)
  • rupeshbio702@gmail.com (Pessoal)

Telefones

Telemóvel
  • 936329756 (Pessoal)

Moradas

  • 114, seg esq, rua do fujacal, 4705-097, Braga, Braga, Portugal (Pessoal)

Websites

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Fitologia
  • Ciências Agrárias - Biotecnologia Agrária e Alimentar - Biotecnologia Agrária e Biotecnologia Alimentar
  • Ciências Agrárias - Agricultura, Silvicultura e Pescas - Horticultura
  • Ciências Agrárias - Agricultura, Silvicultura e Pescas - Viticultura
  • Ciências Agrárias - Agricultura, Silvicultura e Pescas - Produção e Proteção de Plantas
  • Ciências Agrárias - Biotecnologia Agrária e Alimentar - Biotecnologia Agrária e Biotecnologia Alimentar
  • Ciências Agrárias - Biotecnologia Agrária e Alimentar - Tecnologia da Manipulação Genética
  • Ciências da Engenharia e Tecnologias - Biotecnologia Industrial - Biotecnologia Industrial
  • Ciências Naturais - Ciências Biológicas - Botânica

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Inglês Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2)
Português Utilizador proficiente (C1) Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2)
Hindi (Idioma materno)
Formação
Grau Classificação
2007/02/13 - 2011/08/10
Concluído
 Biotechnology (Doktor (PhD))
Especialização em Plant Biotechnology (molecular biology and genetic engineering )
Kumaun University, Índia
"Transcription profiling of low temperature responsive genes of Cicer microphyllum" (TESE/DISSERTAÇÃO)
 qualified
2002/07/02 - 2004/06/30
Concluído
 Master Biotechnology (Master)
Especialização em Plant Biotechnology
Chhatrapati Shahu Ji Maharaj University, Índia
"Molecular marker based diversity analysis in different Chickpea varieties" (TESE/DISSERTAÇÃO)
 First Division
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2023/10/01 - Atual Investigador (Investigação) Universidade de Trás-os-Montes e Alto Douro, Portugal
2023/01/01 - Atual Investigador (Investigação) Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
2022/01/01 - Atual Investigador (Investigação) InnovPlantProtect Collaborative Laboratory, Department of Protection of Specific Crops, Elvas, Portuga, Portugal
2021/01/01 - 2021/12/31 Investigador (Investigação) Universidade de Trás-os-Montes e Alto Douro Centro de Química Vila Real, Portugal
2020/10/01 - 2020/12/31 Investigador principal (carreira) (Investigação) Monaghan Biosciences, Irlanda
2016/07/12 - 2020/03/31 Pós-doutorado (Investigação) Universidade de Trás-os-Montes e Alto Douro Centro de Química Vila Real, Portugal
2013/09/13 - 2016/01/31 Pós-doutorado (Investigação) Universidade do Minho Departamento de Biologia, Portugal
Projetos

Bolsa

Designação Financiadores
2024/08/02 - 2028/06/02 “Holistic evaluation of agroecological measures” (HEAL; ID197)
HEAL; ID197
Bolseiro de Cientista Convidado
Alanus Hochschule gGmbH - Campus II Bonn Brühler Strasse, Alemanha
 Conseil de l'Union Europeenne
Em curso

Projeto

Designação Financiadores
2023/10/01 - 2026/04/30 ECONUTRI, HORIZON-CL6-2022-ZEROPOLLUTION-01
101081858
Bolseiro de Integração na Investigação
Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
Em curso
2016/07/13 - 2020/03/31 INNOVINE&WINE, n.º da operação NORTE-01-0145-FEDER-000038
NORTE- 01-0145-FEDER-000038
Investigador Pós-doutorado
Universidade de Trás-os-Montes e Alto Douro Centro de Ciência Animal e Veterinária, Portugal
Concluído
2013/09/02 - 2015/04/30 Molecular mechanisms of Agrobacterium recognition and defense activation in recalcitrant plant species
UMINHO/BI3-M/CITAB/2013/PTDC/AGR-GPL /119211/2010
Bolseiro de Pós-Doutoramento
Universidade do Minho Centro de Biologia Funcional de Plantas, Portugal
Concluído
Produções

Publicações

Artigo em boletim informativo
  1. Singh, Rupesh. "Associação de antocianinas-ácido ferúlico de castas tintas portuguesas para produzir corantes alimentares naturais", TecnoAlimentar - Revista da Indústria Alimentar, 2019, http://www.tecnoalimentar.pt/noticias/associacao-de-antocianinas-acido-ferolico-de-castas-tintas-portuguesas-para-produzir-corantes-alimentares-naturais/.
Artigo em conferência
  1. RUPESH KUMAR SINGH. "Exploring Winemaking By-Products of Tinto Cão Grapes: Antioxidant and Antimicrobial Activity against Multiresistant Bacteria". Trabalho apresentado em The 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action and Mechanisms of Resistance, 2023.
  2. Goufo, Piebiep; Singh, Rupesh Kumar; Cortez, Isabel; Piebiep Goufo; Rupesh kumar singh; Isabel Cortez. "Metabolites Differentiating Asymptomatic and Symptomatic Grapevine Plants (Vitis vinifera ‘Malvasia-Fina’) Infected with Esca Complex Disease-Associated Fungi". 2021.
    10.3390/iecps2021-11923
  3. Silva, Vanessa; Peirone, Cecília; Singh, Rupesh; Falco, Virgílio; Pires, Isabel; Miranda, Carla; Maltez, Luís; et al. "In vitro evaluation of antibacterial activity and wound healing power of a gel based on phenolic compounds extracted from by-products of the wine industry". Trabalho apresentado em 6th International Electronic Conference on Medicinal Chemistry, 2020.
    Publicado • 10.3390/ecmc2020-07264
  4. Ribeiro, Jessica; Silva, Vanessa; Singh, Rupesh; AIres, Alfredo; Carvalho, Rosa; Falco, Virgílio; Igrejas, Gilberto; Poeta, Patrícia. "Antimicrobial and Antioxidant Activity of Phenolic Compounds Extracted from Wine Industry By-Products of <em>Tinto Cão</em> Variety". Trabalho apresentado em 1st International Electronic Conference on Microbiology, 2020.
    10.3390/ecm2020-07137
  5. Singh, Rupesh. "Chitosan upregulates the genes of the ROS pathway and enhances the antioxidant potential of grapes". Trabalho apresentado em Spanish edition of Enoforum 2020, Zaragoza, 2020.
    Publicado
  6. Singh, Rupesh. "Chitosan applications in vineyards and effects on gene expression". Trabalho apresentado em The Spanish edition of Enoforum 2020, 2020.
    https://doi.org/10.53144/INFOWINE.EN.2021.04.02
  7. Singh, Rupesh. "Molecular and biochemical insight upon chitosan application on vitis vinifera L. CV. Touriga Franca and Tinto Cao". Trabalho apresentado em ENOFORUM WEB, May 5-7, 2020, 2020.
    Publicado
  8. Singh, Rupesh. "Effect of foliar mitigation treatments on Touriga Nacional grape berry quality". Trabalho apresentado em 14º Encontro Nacional de Química dos Alimentos, Viana do Castelo, 2018.
    Publicado
  9. Singh, Rupesh. "Increased accumulation of anthocyanins in vine stems upon chitosan application: alternate use of winery waste produce to extract natural colour additives for the food industry". Trabalho apresentado em XIV Encontro de Química dos AlimentosAt, Viana do Castelo, 2018.
    Publicado
  10. Singh, Rupesh. "Lignin and flavonoid content increases in Hypericum perforatum cell wall after Agrobacterium tumefaciens co-cultivation". 2014.
    10.1055/s-0034-1394589
  11. Singh, Rupesh. "Agrobacterium- elicited Hypericum perforatum cell methanolic extract shows antibacterial activity against human pathogens.". 2014.
    10.1055/s-0034-1394698
Artigo em revista
  1. Vishnu D. Rajput; Abhishek Singh; Aishwarya Sharma; Gang Wang; Hasmik S. Movsesyan; Tatiana Minkina; Priyadarshani Rajput; et al. "Nano-enabled solutions for sustainable agriculture by managing abiotic stress: Critical evaluation on unraveling the pros and cons with ecological impacts". Plant Stress 18 (2025): 101060. https://doi.org/10.1016/j.stress.2025.101060.
    10.1016/j.stress.2025.101060
  2. Roghayyeh Hazrati; Nasser Zare; Rasool Asghari-Zakaria; Parisa Sheikhzadeh; Mahipal S. Shekhawat; Rupesh Kumar Singh. "Enhancing taxane production in Corylus avellana L. cell cultures through elicitation using molybdenum oxide and iron oxide nanoparticles". Industrial Crops and Products (2025): https://doi.org/10.1016/j.indcrop.2025.122269.
    10.1016/j.indcrop.2025.122269
  3. Singh, Rupesh Kumar; Pradhan, Arunava; Falco, Virgílio; Saraiva, Francisco; Cláudia Pascoal; Trindade, Henrique; Fernanda Cássio; Sousa, João Ricardo. "Can nature-based biochar and biochar nanoparticles diminish the impacts of silver nanoparticles and microplastics on microbially-driven stream detrital ecosystem?". Journal of Hazardous Materials Advances 20 (2025): 100903. https://doi.org/10.1016/j.hazadv.2025.100903.
    10.1016/j.hazadv.2025.100903
  4. Sousa, João Ricardo; Ferreira, João; Saraiva, Francisco; Singh, Rupesh Kumar; Singh, Abhishek; Ghazaryan, Karen; Trindade, Henrique. "Soil Amendments for the Remediation of Cu-Contaminated Vineyard Soil: Effects of Biochar, Nanobiochar and Chitosan on Copper Bioavailability and Ecotoxicity". Journal of Innovative Solutions for Eco-Environmental Sustainability (2025): 033. https://doi.org/10.46991/jisees.2025.si1.033.
    Publicado • 10.46991/jisees.2025.si1.033
  5. Vardumyan, Hasmik; Singh, Abhishek; Singh, Rupesh Kumar; Sousa, João Ricardo; Ghazaryan, Karen. "Assessment of Heavy Metals Contamination in Soils of Mining Area of Tumanyan Region, Armenia: Distribution and Environmental Risks". Journal of Innovative Solutions for Eco-Environmental Sustainability (2025): 161. https://doi.org/10.46991/jisees.2025.si1.161.
    10.46991/jisees.2025.si1.161
  6. Narváez-Zapata, José Alberto; Alaníz-Cuevas, Yadira; Chávez-Medina, J. Alicia; Espinoza-Mancillas, Mariela Guadalupe; Maldonado-Mendoza, Ignacio E.; Singh, Rupesh Kumar; Quiroz-Figueroa, Francisco Roberto. "Unveiling the molecular arsenal: genome sequencing and in silico secretome analysis of Fusarium verticillioides provide insights into maize root rot pathogenesis". Frontiers in Plant Science 16 (2025): https://doi.org/10.3389/fpls.2025.1672761.
    10.3389/fpls.2025.1672761
  7. Manokari M.; Mohammad Faisal; Abdulrahman A. Alatar; Rupesh Kumar Singh; Mahipal S. Shekhawat. "Mitigation of shoot tip necrosis in Pisonia grandis R. Br. through modified concentrations of calcium chloride in nutrient medium". Journal of Plant Nutrition (2025): 1-11.
    10.1080/01904167.2025.2563291
  8. Manokari, M.; Prajapati, Sujitkumar R.; Patel, Rohitkumar M.; Jani, Minal D.; Faisal, Mohammad; Alatar, Abdulrahman A.; Singh, Rupesh Kumar; Shekhawat, Mahipal S.. "In vitro regeneration for ex situ conservation of Ochradenus lakhpatensis R.M. Patel & Prajapati (Resedaceae) - an endangered shrub of Gujarat, India". Plant Cell, Tissue and Organ Culture (PCTOC) 162 3 (2025): https://doi.org/10.1007/s11240-025-03208-x.
    10.1007/s11240-025-03208-x
  9. M. Manokari; Mohammad Faisal; Abdulrahman A. Alatar; Rupesh Kumar Singh; Mahipal S. Shekhawat. "Polyethylene glycol (PEG) improved macro- and micro-morphological traits of in vitro shoots of Hedyotis biflora (L.) Lam.". Acta Physiologiae Plantarum (2025): https://doi.org/10.1007/s11738-025-03827-z.
    10.1007/s11738-025-03827-z
  10. Singh, Abhishek; Bol, Roland; Lovynska, Viktoriia; Singh, Rupesh Kumar; Sousa, João Ricardo; Ghazaryan, Karen. "Application of nanoparticles for salinity stress management and biofortification in wheat: a review of dual approaches and insights". Frontiers in Plant Science 16 (2025): https://doi.org/10.3389/fpls.2025.1592866.
    10.3389/fpls.2025.1592866
  11. M., Manokari; Jogam, Phanikanth; Faisal, Mohammad; Alatar, Abdulrahman A; Singh, Rupesh Kumar; Shekhawat, Mahipal S. "Meta-Topolin-induced organogenesis in Sphagneticola trilobata (L.) Pruski via fascicular cambium activation and transverse thin cell layers". In Vitro Cellular & Developmental Biology - Plant (2025): https://doi.org/10.1007/s11627-025-10559-9.
    10.1007/s11627-025-10559-9
  12. Singh, Rupesh. "Sustainable Management of Saline Soils: Insights into Organic Amendments for Enhancing Soil Health and Crop Resilience". Biogeosystem Technique 12 (2025):
    Acesso aberto • 10.13187/bgt.2025.1.36
  13. Shipra Singh Parmar; Ramesh Kumar; Amit Vikram; Rajesh Kumar Dogra; Meenu Gupta; Abhishek Singh; Karen Ghazaryan; Rupesh Kumar Singh; João Ricardo Sousa. "Correlation, Path-Coefficient, and Economic Heterosis Studies in CMS-Based Cabbage Hybrids over Different Environments". Horticulturae (2025): https://doi.org/10.3390/horticulturae11060606.
    10.3390/horticulturae11060606
  14. Singh, Abhishek; Singh, Anil Kumar; Rawat, Sapna; Rajput, Vishnu D.; Ghazaryan, Karen; Anand, Vicky; Minkina, Tatiana; et al. "Temporal patterns and influences of monthly, seasonal and annual temperatures on methane emissions in Greece, Armenia and Russia over two decades". Science of The Total Environment 978 (2025): 179428. https://doi.org/10.1016/j.scitotenv.2025.179428.
    10.1016/j.scitotenv.2025.179428
  15. Rupesh Kumar Singh; Vasiliy A. Chokheli. "Plant Biotechnology: Applications in In Vitro Plant Conservation and Micropropagation". Horticulturae (2025): https://doi.org/10.3390/horticulturae11040358.
    10.3390/horticulturae11040358
  16. Paula Cristina Marinho Silva; Francisco Saraiva; Rupesh Kumar Singh; Vishnu D. Rajput; Henrique Trindade; João Ricardo Sousa; Marina Burachevskaya. "Mitigation of earthworm behavior against lithium pollution using biochar". EURASIAN JOURNAL OF SOIL SCIENCE (EJSS) (2025): https://doi.org/10.18393/ejss.1618861.
    10.18393/ejss.1618861
  17. Singh, Abhishek; Sharma, Ragini; Singh, Sakshi; Singh, Rupesh Kumar; Alexiou, Athanasios; Sousa, João Ricardo; El-Ramady, Hassan; et al. "Addressing Abiotic Stresses and Advancing SDGs by Biochar for Sustainable Agriculture and Environmental Restoration". Egyptian Journal of Soil Science 65 1 (2025): 0-0. https://doi.org/10.21608/ejss.2025.340493.1927.
    10.21608/ejss.2025.340493.1927
  18. Mingjun Pu; Yingyu Zhang; Santanu Mukherjee; Saif F. Alharbi; Rupesh Kumar Singh; Salah F. Abou-Elwafa; Henrique Trindade; Tao Zhang. "Impact of Combined Application of Swine Manure Liquid and Phosphorus Fertilizers on Soil Phosphorus and Microbial Communities". Sustainability (2025): https://doi.org/10.3390/su17052037.
    10.3390/su17052037
  19. Singh, Rupesh. "Machine learning-enabled optimization of biochar resource utilization and carbon mitigation pathways: mechanisms and challenges". BIOCHAR X e002 (2025): https://www.maxapress.com/article/doi/10.48130/bchax-0025-0003.
    Acesso aberto • Publicado
  20. Ghazaryan, Karen; Agrawal, Shreni; Margaryan, Gohar; Harutyunyan, Anna; Rajput, Priyadarshani; Movsesyan, Hasmik; Rajput, Vishnu D.; et al. "Soil pollution: an agricultural and environmental problem with nanotechnological remediation opportunities and challenges". Discover Sustainability 5 1 (2024): https://doi.org/10.1007/s43621-024-00666-9.
    No prelo • 10.1007/s43621-024-00666-9
  21. Singh, Abhishek; Rawat, Sapna; Rajput, Vishnu D.; Minkina, Tatiana; Mandzhieva, Saglara; Eloyan, Arevik; Singh, Rupesh Kumar; et al. "Nanotechnology Products in Agriculture and Environmental Protection: Advances and Challenges". Egyptian Journal of Soil Science 64 4 (2024): 0-0. http://dx.doi.org/10.21608/ejss.2024.300047.1802.
    10.21608/ejss.2024.300047.1802
  22. Manokari, M.; Faisal, Mohammad; Alatar, Abdulrahman A.; Singh, Rupesh Kumar; Shekhawat, Mahipal S.. "Nano-silicon stabilized biometric and foliar micro-morpho-anatomical traits during in vitro propagation of Cyperus rotundus L. – A model C4 ecotype". Industrial Crops and Products 218 (2024): 118852. http://dx.doi.org/10.1016/j.indcrop.2024.118852.
    10.1016/j.indcrop.2024.118852
  23. João Ricardo Sousa; Abhishek Singh; Karen Ghazaryan; Rupesh Kumar Singh; Vishnu D. Rahput ; Henrique Trindade; j. Coutinho; Vishnu D. Rahput; J. Coutinho. Autor correspondente: Rupesh Kumar Singh. "Prediction of mass loss in different organic materials under field Mediterranean conditions: influence of the initial chemical quality". Journal of Ecological Engineering (2024): https://doi.org/10.12911/22998993/200512.
    No prelo • 10.12911/22998993/200512
  24. Lenar R. Valiullin; Ascar R. Gibadullin; Vladislav I. Egorov; Rinat S. Mukhammadiev; Rishat S. Mukhammadiev; Vladimir V. Sakhnov; Rupesh Kumar Singh; et al. "Ensuring Tree Protection, Growth and Sustainability by Microbial Isolates". Sustainability (2024): https://doi.org/10.3390/su16177837.
    10.3390/su16177837
  25. Singh, Abhishek; Rajput, Vishnu D.; Agrawal, Shreni; Ghazaryan, Karen; Minkina, Tatiana; Al Tawaha, Abdel Rahman Mohammad; Chauhan, Avnish; et al. "Nanoparticles Mediated Salt Stress Resilience: A Holistic Exploration of Physiological, Biochemical, and Nano-omics Approaches". Reviews of Environmental Contamination and Toxicology 262 1 (2024): http://dx.doi.org/10.1007/s44169-024-00070-4.
    10.1007/s44169-024-00070-4
  26. Ghazaryan, Karen; Pandey, Divya; Singh, Sakshi; Varagyan, Vahagn; Alexiou, Athanasios; Petropoulos, Dimitrios; Kriemadis, Athanasios; et al. "Enhancing Crop Production: Unveiling the Role of Nanofertilizers in Sustainable Agriculture and Precision Nutrient Management". Egyptian Journal of Soil Science 64 3 (2024): 0-0. http://dx.doi.org/10.21608/ejss.2024.282778.1748.
    10.21608/ejss.2024.282778.1748
  27. João Ricardo Sousa; Abhishek Singh; Karen Ghazaryan; Rupesh Kumar Singh; Henrique Trindade; João Coutinho. "In-vitro Digestibility Organic Materials – Relation with Field Mass Loss Litter Bag Method". Journal of Ecological Engineering (2024): https://doi.org/10.12911/22998993/189947.
    10.12911/22998993/189947
  28. Singh, Abhishek; Sharma, Aishwarya; Singh, Omkar; Rajput, Vishnu D.; Movsesyan, Hasmik S.; Minkina, Tatiana; Alexiou, Athanasios; et al. "In-depth Exploration of Nanoparticles for Enhanced Nutrient Use Efficiency and Abiotic Stresses Management: Present Insights and Future Horizons". Plant Stress (2024): 100576. http://dx.doi.org/10.1016/j.stress.2024.100576.
    10.1016/j.stress.2024.100576
  29. Sereda, Mikhail; Petrenko, Victoria; Kapralova, Olga; Chokheli, Vasily; Varduni, Tatyana; Dmitriev, Pavel; Minkina, Tatiana; et al. "Establishment of an In Vitro Micropropagation Protocol for Hibiscus moscheutos L. ‘Berry Awesome’". Horticulturae 10 1 (2023): 21. http://dx.doi.org/10.3390/horticulturae10010021.
    10.3390/horticulturae10010021
  30. M, Cokul Raj; M, Manokari; Dey, Abhijit; Faisal, Mohammad; Alatar, Abdulrahman Abdullah; Singh, Rupesh Kumar; Shekhawat, Mahipal Singh. "Improvements in morphometric and structural traits of Vitex trifolia L. to exogenous application of nano-silicon in vitro". South African Journal of Botany 160 (2023): 613-621. http://dx.doi.org/10.1016/j.sajb.2023.07.049.
    10.1016/j.sajb.2023.07.049
  31. Vinogradova, Natalya; Vinogradova, Elena; Chaplygin, Victor; Mandzhieva, Saglara; Kumar, Pradeep; Rajput, Vishnu D.; Minkina, Tatiana; et al. "Phenolic Compounds of the Medicinal Plants in an Anthropogenically Transformed Environment". Molecules 28 17 (2023): 6322. http://dx.doi.org/10.3390/molecules28176322.
    10.3390/molecules28176322
  32. Ibrahim, Muhammad; Akhtar, Naveed; Wahab, Abdul; Alrefaei, Abdulwahed Fahad; Almutairi, Mikhlid H.. "Exploring Wild Edible Plants in Malakand, Pakistan: Ethnobotanical and Nutritional Insights". Sustainability 15 17 (2023): 12881. http://dx.doi.org/10.3390/su151712881.
    10.3390/su151712881 • Editor
  33. M., Manokari; Dey, Abhijit; Faisal, Mohammad; Alatar, Abdulrahman A.; Singh, Rupesh Kumar; Shekhawat, Mahipal S.. "In vitro Tuberization using Silicon Nanoparticles and short-term cold Storage of mini-tubers of Dioscorea pentaphylla L.". BioNanoScience (2023): http://dx.doi.org/10.1007/s12668-023-01185-z.
    10.1007/s12668-023-01185-z
  34. Quiroz-Figueroa, Francisco Roberto; Cruz-Mendívil, Abraham; Ibarra-Laclette, Enrique; García-Pérez, Luz María; Gómez-Peraza, Rosa Luz; Hanako-Rosas, Greta; Ruíz-May, Eliel; et al. "Cell wall-related genes and lignin accumulation contribute to the root resistance in different maize (Zea mays L.) genotypes to Fusarium verticillioides (Sacc.) Nirenberg infection". Frontiers in Plant Science 14 (2023): http://dx.doi.org/10.3389/fpls.2023.1195794.
    10.3389/fpls.2023.1195794
  35. Manokari, M.; Raj, M. Cokul; Dey, Abhijit; Faisal, Mohammad; Alatar, Abdulrahman A.; Singh, Rupesh Kumar; Arumugam, N.; Shekhawat, Mahipal S.. "Development of stress tolerance in micropropagated plantlets of Dioscorea pentaphylla L. using seismic stress". Plant Cell, Tissue and Organ Culture (PCTOC) (2023): http://dx.doi.org/10.1007/s11240-023-02549-9.
    10.1007/s11240-023-02549-9
  36. Manokari, M.; Dey, Abhijit; Faisal, Mohammad; Alatar, Abdulrahman A.; Singh, Rupesh Kumar; Shekhawat, Mahipal S.. "Silicon Nanoparticles (SiNPs) Positively Affect Morpho-Structural Differentiation in Micropropagated Plantlets of Santalum album L.". Silicon (2023): http://dx.doi.org/10.1007/s12633-023-02558-5.
    10.1007/s12633-023-02558-5
  37. Singh, Rupesh. "Polyethylene glycol-mediated drought stress improved macro- and micro-morphology of leaves of Hedyotis biflora (L.) Lam. in vitro". Plant cell tissue and organ culture (2023):
    Aceite para publicação
  38. Manokari, M.; Cokul Raj, M.; Dey, Abhijit; Faisal, Mohammad; Alatar, Abdulrahman A.; Singh, Rupesh Kumar; Shekhawat, Mahipal S.. "Silicon Nanoparticles Moderated Morphometric Deficiencies by Improving Micro-Morpho-Structural Traits in Thunbergia erecta (Benth.) T. Anderson". Silicon (2023): http://dx.doi.org/10.1007/s12633-023-02451-1.
    10.1007/s12633-023-02451-1
  39. Bhattacharjee, Subhradip; Panja, Amitava; Panda, Moumita; Dutta, Subham; Dutta, Susanta; Kumar, Rakesh; Kumar, Dinesh; et al. "How Did Research on Conservation Agriculture Evolve over the Years? A Bibliometric Analysis". Sustainability 15 3 (2023): 2040. http://dx.doi.org/10.3390/su15032040.
    10.3390/su15032040
  40. Manokari, M.; Cokulraj, M.; Badhepuri, Mahesh Kumar; Dey, Abhijit; Faisal, Mohammad; Alatar, Abdulrahman A.; Singh, Rupesh Kumar; Shekhawat, Mahipal S.. "Microstructural and histochemical modifications in leaves at successive stages of in vitro development of the terrestrial orchid Spathoglottis plicata Blume". Horticulture, Environment, and Biotechnology (2023): http://dx.doi.org/10.1007/s13580-022-00485-9.
    10.1007/s13580-022-00485-9
  41. Lysenko, Vladimir; D. Rajput, Vishnu; Kumar Singh, Rupesh; Guo, Ya; Kosolapov, Alexey; Usova, Elena; Varduny, Tatyana; et al. "Chlorophyll fluorometry in evaluating photosynthetic performance: key limitations, possibilities, perspectives and alternatives". Physiology and Molecular Biology of Plants 28 11-12 (2022): 2041-2056. http://dx.doi.org/10.1007/s12298-022-01263-8.
    Publicado • 10.1007/s12298-022-01263-8
  42. Sumira Malik; archna dhasmana; Subham Preetam; Prof. Yogendra Kumar Mishra; Vishal Chaudhary; Sweta Parmita Bera; Anuj Ranjan; et al. "Exploring Microbial-Based Green Nanobiotechnology for Wastewater Remediation: A Sustainable Strategy". Nanomaterials (2022): https://www.mdpi.com/2079-4991/12/23/4187.
    10.3390/nano12234187
  43. Rupesh Kumar Singh; Eliel Ruiz-May; Vishnu D. Rajput; Tatiana Minkina; Rosa Luz Gómez-Peraza; Krishan K. Verma; Mahipal S. Shekhawat; et al. Autor correspondente: Rupesh Kumar Singh. "Viewpoint of Chitosan Application in Grapevine for Abiotic Stress/Disease Management towards More Resilient Viticulture Practices". Agriculture (2022): https://doi.org/10.3390/agriculture12091369.
    10.3390/agriculture12091369
  44. Francisco R. Quiroz-Figueroa; Juan Luis Monribot-Villanueva; Esaú Bojórquez-Velázquez; Rosa L. Gómez-Peraza; José Miguel Elizalde Contreras; Mirna V. Bautista-Valle; José A. Guerrero-Analco; et al. "Proteometabolomic Analysis Reveals Molecular Features Associated with Grain Size and Antioxidant Properties amongst Chickpea (Cicer arietinum L.) Seeds Genotypes". Antioxidants (2022): https://www.mdpi.com/2076-3921/11/10/1850.
    10.3390/antiox11101850
  45. Abhishek singh; R.S. Sengar; Vishnu D Rajput; Tatiana Minkina; Rupesh kumar singh. "Zinc Oxide Nanoparticles Improve Salt Tolerance in Rice Seedlings by Improving Physiological and Biochemical Indices". Agriculture (2022): https://www.mdpi.com/2077-0472/12/7/1014.
    10.3390/agriculture12071014
  46. M, Manokari; Badhepuri, Mahesh Kumar; M, Cokulraj; Sandhya, Dulam; Dey, Abhijit; Kumar, Vijay; Faisal, Mohammad; et al. "Validation of meta-Topolin in organogenesis, improved morpho-physio-chemical responses, and clonal fidelity analysis in Dioscorea pentaphylla L. – an underutilized yam species". South African Journal of Botany 145 (2022): 284-292. http://dx.doi.org/10.1016/j.sajb.2021.12.039.
    10.1016/j.sajb.2021.12.039
  47. Vishnu D. Rajput; Tatiana Minkina; Sudhir K. Upadhyay; Arpna Kumari; Anuj Ranjan; Saglara Mandzhieva; Svetlana Sushkova; Rupesh Kumar Singh; Krishan K. Verma. "Nanotechnology in the Restoration of Polluted Soil". Nanomaterials 12 5 (2022): 769-769. https://doi.org/10.3390/nano12050769.
    10.3390/nano12050769
  48. Verma, Krishan K.; Song, Xiu-Peng; Tian, Dan-Dan; Guo, Dao-Jun; Chen, Zhong-Liang; Zhong, Chang-Song; Nikpay, Amin; et al. "Influence of Silicon on Biocontrol Strategies to Manage Biotic Stress for Crop Protection, Performance, and Improvement". Plants 10 10 (2021): 2163. https://doi.org/10.3390/plants10102163.
    doi.org/10.3390/plants10102163
  49. Kolesnikov, Sergey; Timoshenko, Alena; Minnikova, Tatiana; Tsepina, Natalia; Kazeev, Kamil; Akimenko, Yulia; Zhadobin, Alexander; et al. "Impact of Metal-Based Nanoparticles on Cambisol Microbial Functionality, Enzyme Activity, and Plant Growth". Plants 10 10 (2021): 2080. http://dx.doi.org/10.3390/plants10102080.
    10.3390/plants10102080
  50. Singh, Rupesh. "Interaction between Arbuscular Mycorrhiza and Rhizobacteria with Legumes : A Promising Strategy to Fight with Abiotic Stress". World Journal of Agriculture and Soil Science 7 3 (2021):
    Publicado
  51. Vishnu D Rajput; Tatiana Minkina; Morteza Feizi; Arpna Kumari; Masudulla Khan; Saglara Mandzhieva; Svetlana Sushkova; et al. "Effects of Silicon and Silicon-Based Nanoparticles on Rhizosphere Microbiome, Plant Stress and Growth". Biology (2021): https://www.mdpi.com/2079-7737/10/8/791.
    10.3390/biology10080791
  52. Hou, Weina; Singh, Rupesh K.; Martins, Viviana; Tenllado, Francisco; Franklin, Gregory; Dias, Alberto C. P.. "Transcriptional responses of Hypericum perforatum cells to Agrobacterium tumefaciens and differential gene expression in dark glands". Functional Plant Biology 48 9 (2021): 936-947. http://dx.doi.org/10.1071/fp20292.
    Publicado • 10.1071/fp20292
  53. Prof. G.S. Shekhawat; Lovely Mahawar; Priyadarshani Rajput; Vishnu D Rajput; Tatiana Minkina; Rupesh kumar singh. "Role of Engineered Carbon Nanoparticles (CNPs) in Promoting Growth and Metabolism of Vigna radiata (L.) Wilczek: Insights into the Biochemical and Physiological Responses". Plants (2021): https://www.mdpi.com/2223-7747/10/7/1317.
    10.3390/plants10071317
  54. Sergey Kolesnikov; Natalia Tsepina; Tatyana Minnikova; Kamil Kazeev; Saglara Mandzhieva; Svetlana Sushkova; Tatiana Minkina; et al. "Influence of Silver Nanoparticles on the Biological Indicators of Haplic Chernozem". Plants (2021): https://www.mdpi.com/2223-7747/10/5/1022.
    10.3390/plants10051022
  55. Vishnu D Rajput; Harish; Rupesh kumar singh; Dr. Krishan Kumar Verma; Lav Sharma; Francisco Roberto Quiroz-Figueroa; Mukesh Meena; et al. Autor correspondente: Rupesh kumar singh. "Recent Developments in Enzymatic Antioxidant Defence Mechanism in Plants with Special Reference to Abiotic Stress". Biology (2021): https://www.mdpi.com/2079-7737/10/4/267.
    10.3390/biology10040267
  56. Lav Sharma; Irene Oliveira; Fátima Gonçalves; Fernando Raimundo; Rupesh kumar singh; Laura Torres; Guilhermina Marques. "Effect of Soil Chemical Properties on the Occurrence and Distribution of Entomopathogenic Fungi in Portuguese Grapevine Fields". Pathogens (2021): https://www.mdpi.com/2076-0817/10/2/137.
    10.3390/pathogens10020137
  57. Sharma, Lav; Bohra, Nitin; Rajput, Vishnu D.; Quiroz-Figueroa, Francisco Roberto; Singh, Rupesh Kumar; Marques, Guilhermina. "Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi". Microorganisms 9 1 (2020): 16. http://dx.doi.org/10.3390/microorganisms9010016.
    10.3390/microorganisms9010016
  58. Román, Shamir Gabriel; Quiroz-Chávez, Jesús; Villalobos, Miguel; Urías-Gutiérrez, Vianey; Nava-Pérez, Eusebio; Ruíz-May, Eliel; Singh, Rupesh Kumar; et al. "A Global Screening Assay to Select for Maize Phenotypes with a High Tolerance or Resistance to Fusarium verticillioides (Sacc.) Nirenberg Rots". Agronomy 10 12 (2020): 1990. http://dx.doi.org/10.3390/agronomy10121990.
    Acesso aberto • 10.3390/agronomy10121990
  59. Chokheli, Vasiliy A.; Dmitriev, Pavel A.; Rajput, Vishnu D.; Bakulin, Semyon D.; Azarov, Anatoly S.; Varduni, Tatiana V.; Stepanenko, Victoria V.; et al. "Recent Development in Micropropagation Techniques for Rare Plant Species". Plants 9 12 (2020): 1733. http://dx.doi.org/10.3390/plants9121733.
    10.3390/plants9121733
  60. Hou, Weina; Singh, Rupesh Kumar; Zhao, Pan; Martins, Viviana; Aguilar, Emmanuel; Canto, Tomás; Tenllado, Francisco; Franklin, Gregory; Dias, Alberto Carlos Pires. "Overexpression of polygalacturonase-inhibiting protein (PGIP) gene from Hypericum perforatum alters expression of multiple defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens in tobacco". Journal of Plant Physiology 253 (2020): 153268. http://dx.doi.org/10.1016/j.jplph.2020.153268.
    10.1016/j.jplph.2020.153268
  61. Meza-Menchaca, Thuluz; Singh, Rupesh Kumar; Quiroz-Chávez, Jesús; García-Pérez, Luz María; Rodríguez-Mora, Norma; Soto-Luna, Manuel; Gastélum-Contreras, Guadalupe; et al. "First Demonstration of Clinical Fusarium Strains Causing Cross-Kingdom Infections from Humans to Plants". Microorganisms 8 6 (2020): 947. http://dx.doi.org/10.3390/microorganisms8060947.
    10.3390/microorganisms8060947
  62. Goufo, Piebiep; Singh, Rupesh Kumar; Cortez, Isabel. "A Reference List of Phenolic Compounds (Including Stilbenes) in Grapevine (Vitis vinifera L.) Roots, Woods, Canes, Stems, and Leaves". Antioxidants 9 5 (2020): 398. http://dx.doi.org/10.3390/antiox9050398.
    10.3390/antiox9050398
  63. Singh, Rupesh Kumar; Afonso, Jessica; Nogueira, Marta; Oliveira, Ana A.; Cosme, Fernanda; Falco, Virgílio. Autor correspondente: Singh, Rupesh Kumar. "Silicates of Potassium and Aluminium (Kaolin); Comparative Foliar Mitigation Treatments and Biochemical Insight on Grape Berry Quality in Vitis vinifera L. (cv. Touriga National and Touriga Franca)". Biology 9 3 (2020): 58. http://dx.doi.org/10.3390/biology9030058.
    10.3390/biology9030058
  64. Silva, Vanessa; Singh, Rupesh Kumar; Gomes, Nelson; Soares, Bruno Gonçalves; Silva, Adriana; Falco, Virgílio; Capita, Rosa; et al. "Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety". Antioxidants 9 2 (2020): 178. http://dx.doi.org/10.3390/antiox9020178.
    10.3390/antiox9020178
  65. Singh, Rupesh Kumar; Martins, Viviana; Soares, Bruno; Castro, Isaura; Falco, Virgílio. "Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes". International Journal of Molecular Sciences 21 1 (2020): 306. http://dx.doi.org/10.3390/ijms21010306.
    10.3390/ijms21010306
  66. Hou, Weina; Singh, Rupesh Kumar; Zhao, Pan; Martins, Viviana; Aguilar, Emmanuel; Canto, Tomás; Tenllado, Francisco; Dias, Alberto Carlos Pires. "Transgenic expression of Hyp-1 gene from Hypericum perforatum L. alters expression of defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens". Planta 251 1 (2019): http://dx.doi.org/10.1007/s00425-019-03310-3.
    10.1007/s00425-019-03310-3
  67. Singh; Soares; Goufo; Castro; Cosme; Pinto-Sintra; Inês; Oliveira; Falco. Autor correspondente: Singh. "Chitosan Upregulates the Genes of the ROS Pathway and Enhances the Antioxidant Potential of Grape (Vitis vinifera L. ‘Touriga Franca’ and ’Tinto Cão’) Tissues". Antioxidants 8 11 (2019): 525. http://dx.doi.org/10.3390/antiox8110525.
    10.3390/antiox8110525
  68. Singh, Rupesh Kumar; Anandhan, Sivalingam; García-Pérez, Luz María; Ruiz-May, Eliel; Nava Pérez, Eusebio; Quiroz-Figueroa, Francisco Roberto. "An efficient protocol for in vitro propagation of the wild legume Cicer microphyllum Benth., a crop wild relative of chickpea (Cicer arietinum L.)". In Vitro Cellular & Developmental Biology - Plant 55 1 (2019): 9-14. http://dx.doi.org/10.1007/s11627-018-09958-y.
    10.1007/s11627-018-09958-y
  69. Singh, Rupesh Kumar; Bohra, Nitin; Sharma, Lav. Autor correspondente: Singh, Rupesh Kumar. "Valorizing faba bean for animal feed supplements via biotechnological approach: Opinion". Biocatalysis and Agricultural Biotechnology 17 (2019): 366-368. http://dx.doi.org/10.1016/j.bcab.2018.12.020.
    10.1016/j.bcab.2018.12.020
  70. Singh, R. K.; Singh, S.; Anandhan, S.; Shannon, L. M.; Quiroz-Figueroa, F. R.; Ruiz-May, E.. "First insights into the biochemical and molecular response to cold stress in Cicer microphyllum, a crop wild relative of chickpea (Cicer arietinum)". Russian Journal of Plant Physiology 64 5 (2017): 758-765. http://dx.doi.org/10.1134/s1021443717050120.
    10.1134/s1021443717050120
  71. Singh, Rupesh Kumar; Singh, Shweta; Pandey, Pankaj; Anandhan, Sivalingam; Goyary, Danswrang; Pande, Veena; Ahmed, Zakwan. Autor correspondente: Singh, Rupesh Kumar. "Construction of cold induced subtracted cDNA library from Cicer microphyllum and transcript characterization of identified novel wound induced gene". Protoplasma 250 2 (2012): 459-469. http://dx.doi.org/10.1007/s00709-012-0429-z.
    10.1007/s00709-012-0429-z
  72. Singh, Shweta; Rathore, Meenal; Goyary, Danswrang; Singh, Rupesh Kumar; Anandhan, Sivalingam; Sharma, Dinesh K.; Ahmed, Zakwan. "Induced ectopic expression of At-CBF1 in marker-free transgenic tomatoes confers enhanced chilling tolerance". Plant Cell Reports 30 6 (2011): 1019-1028. http://dx.doi.org/10.1007/s00299-011-1007-0.
    10.1007/s00299-011-1007-0
  73. Singh, Rupesh K.; Anandhan, Sivalingam; Singh, Shweta; Patade, Vikas Y.; Ahmed, Zakwan; Pande, Veena. "Metallothionein-like gene from Cicer microphyllum is regulated by multiple abiotic stresses". Protoplasma 248 4 (2010): 839-847. http://dx.doi.org/10.1007/s00709-010-0249-y.
    10.1007/s00709-010-0249-y
  74. Gupta, S.M.; Singh, R.K.; Ahmed, Z.; Sanjay Mohan Gupta; Singh, Rupesh; Ahmed, Zakwan. "Isolation and characterization of cold regulated genes from winter wheat (Triticum aesiivum L.) by mRNA differential-display reverse transcriptase-PCR (DDRT-PCR)". Proceedings of the National Academy of Sciences India Section B - Biological Sciences 80 PART 2 (2010): 150-154. http://www.scopus.com/inward/record.url?eid=2-s2.0-80051926777&partnerID=MN8TOARS.
  75. Aslam, Mohammad; Sinha, Vimlendu B.; Singh, Rupesh K.; Anandhan, Sivalingam; Pande, Veena; Ahmed, Zakwan. "Isolation of cold stress-responsive genes from Lepidium latifolium by suppressive subtraction hybridization". Acta Physiologiae Plantarum 32 1 (2009): 205-210. http://dx.doi.org/10.1007/s11738-009-0382-4.
    10.1007/s11738-009-0382-4
  76. Singh, Rupesh. "Micrografting of In-Vitro cultured shoots of Pisum sativum L. onto potted root stocks.". Biochem. Cell. Arch. (2009):
  77. Shah, Pramila; Singh, N. K.; Khare, Neeraj; Rathore, Meenal; Anandhan, S.; Arif, M.; Singh, Rupesh Kumar; et al. "Agrobacterium mediated genetic transformation of summer squash (Cucurbita pepo L. cv. Australian green) with cbf-1 using a two vector system". Plant Cell, Tissue and Organ Culture 95 3 (2008): 363-371. http://dx.doi.org/10.1007/s11240-008-9450-5.
    10.1007/s11240-008-9450-5
Artigo em revista (magazine)
  1. Singh, Rupesh. "Avaliação do impacto do lítio no evitamento da Eisenia fetida: efeito da concentração e do tipo de solo", Revistas da ciencias agrarias, 2025, https://revistas.rcaap.pt/rca/article/view/38549.
  2. Singh, Rupesh. "Efeito da aplicação de biochar (BioC) na mineralização de azoto a partir de matéria orgânica num solo arenoso", Revistas da ciencias agrarias, 2025, https://revistas.rcaap.pt/rca/article/view/38923.
  3. Singh, Rupesh. "Efeito de dois sais de lítio no comportamento da Eisenia andrei em ensaios de evitamento Lithium salt type effect on behaviour of Eisenia andrei in short-test avoidance assay", Revistas da ciencias agrarias, 2025, https://revistas.rcaap.pt/rca/article/view/38922.
Capítulo de livro
  1. Singh, Rupesh. "Nanoparticles: Dual role in alleviating abiotic stresses and boosting nutrient efficiency for sustainable agriculture". Elsevier BV, 2025.
    10.1016/bs.agron.2025.08.001
  2. Choudhury, Simran; Bhattacharya, Indrani; Agrawal, Shreni; Singh, Abhishek; Rajput, Vishnu D.; Singh, Sakshi; Margaryan, Gohar; et al. "Beyond science: ethical and societal considerations in the era of biogenic nanoparticles". In Biogenic Nanoparticles, 55-75. Elsevier, 2025.
    10.1016/b978-0-443-33819-9.00007-x
  3. Farhan, Morishwala Mohammad; Agrawal, Shreni; Bhattacharya, Indrani; Singh, Abhishek; Harutyunyan, Anna; Movsesyan, Hasmik; Singh, Rupesh Kumar; et al. "Adaptation and resilience: plants and microorganism responding to biogenic nanomaterial". In Biogenic Nanoparticles, 227-255. Elsevier, 2025.
    10.1016/b978-0-443-33819-9.00014-7
  4. Choudhury, Simran; Patel, Nishi; Agrawal, Shreni; Bhattacharya, Indrani; Singh, Abhishek; Chakhmakhchyan, Armine; Hayrapetyan, Ani; et al. "Nanoshields: harnessing biogenic nanomaterials to combat the adverse effects of heavy metal stress". In Biogenic Nanoparticles, 323-344. Elsevier, 2025.
    10.1016/b978-0-443-33819-9.00017-2
  5. Solanki, Shivangee; Agrawal, Shreni; Bhattacharya, Indrani; Singh, Abhishek; Nare, Darbinyan; Singh, Rupesh Kumar; Quiroz-Figueroa, Francisco Roberto; et al. "Foliar and soil application dynamics: investigating the transport and mechanism of biogenic nanoparticles in the plant tissues". In Biogenic Nanoparticles, 209-226. Elsevier, 2025.
    10.1016/b978-0-443-33819-9.00013-5
  6. Abhishek Singh; Bhavana Tomar; Anna Harutyunyan; Priyadarshani Rajput; Tatiana Minkina; Saglara Mandzhieva; Mohamed S. Elshikh; et al. "Utilizing Nanotechnology in Agriculture". In A Balancing Approach Between Environmental Health and Risks. 2024.
    10.4018/979-8-3693-1471-5.ch019
  7. Aishwarya Sharma; Abhishek Singh; Priyadarshani Rajput; Tatiana Minkina; Saglara Mandzhieva; Mohamed S. Elshikh; Shen-Ming Chena; et al. "Revolutionizing Agricultural Sustainability and Food Security and Management to Achieve SDGs Goals via Nanotechnology". 2024.
    10.4018/979-8-3693-1471-5.ch013
  8. Karen Ghazaryan; Aman Verma; Sapna Rawat; Priyadarshani Rajput; Tatiana Minkina; Saglara Mandzhieva; Mohamed S. Elshikh; et al. "Implementing Sustainable Nano-Bioremediation for Emerging Pollutants". In An Environmentally Friendly Remediation Strategy. 2024.
    10.4018/979-8-3693-1471-5.ch016
  9. Abhishek Singh; Bhavana Tomar; Gohar Margaryan; Priyadarshani Rajput; Tatiana Minkina; Saglara Mandzhieva; Mohamed S. Elshikh; et al. "Emerging Technologies for Sustainable Soil Management and Precision Farming". 2024.
    10.4018/979-8-3693-1471-5.ch010
  10. Aman Verma; Abhishek Singh; Sapna Rawat; Priyadarshani Rajput; Tatiana Minkina; Saglara Mandzhieva; Mohamed S. Elshikh; et al. "Unveiling Nanomaterial-Induced Toxicity". In Navigating Challenges to Modern Cultivation and Soil Management Practices. 2024.
    10.4018/979-8-3693-1471-5.ch011
  11. Karen Ghazaryan; Aman Verma; Sapna Rawat; Amrit Warshini; Priyadarshani Rajput; Tatiana Minkina; Mohamed S. Elshikh; et al. "Green Remediation Harnessing Plant-Based Strategies for Removal of Emerging Soil Contaminants". 2024.
    10.4018/979-8-3693-1471-5.ch004
  12. Vishnu D. Rajput; Abhishek Singh; Bhavana Tomar; Tatiana Minkina; Hasmik S. Movsesyan; Mohamed S. Elshikh; Shen-Ming Chena; Rupesh Kumar Singh; Karen Ghazaryan. "Nanoparticle-Mediated Approaches in Agriculture Addressing Abiotic Stress From Soil to Plant Cells". 2024.
    10.4018/979-8-3693-1471-5.ch005
  13. Singh, Rupesh. "Invasive Plant Pathogens and Their Impacts on Major Crops: Identifying the Worst Offenders of the European Union". In Sustainable Management of Invasive Species. World Scientific Publishing Europe Ltd., 2024.
    Aceite para publicação
  14. Singh, Rupesh. "Global Status of Commercial Organic and Nano-Agri-Inputs and Trade Practices". In Organic and Biogenic Nano-Agri-Inputs in Sustainable Crop Production. Nova Science Publishers, 2024.
    Aceite para publicação
  15. Kumar, Sumit; Rahman, Mehjebin; Gayithri, Mateti; Anjali; Bouket, Ali Chenari; Naveenkumar, R.; Ranjan, Anuj; et al. "Thermophilic Microbes: Their Role in Plant Growth Promotion and Mitigation of Biotic Stress". In Extremophiles for Sustainable Agriculture and Soil Health Improvement, 337-359. Springer Nature Switzerland, 2024.
    10.1007/978-3-031-70203-7_15
  16. Singh, Shweta; Sharma, Lav; Singh, Rupesh Kumar; Rajput, Vishnu D.; Sushkova, Svetlana; Minkina, Tatiana; Verma, Krishan K.. Autor correspondente: Singh, Rupesh Kumar. "Bacillus-Based Biocontrol". In Microbial Based Land Restoration Handbook, Volume 2, 161-176. CRC Press, 2023.
    Publicado • 10.1201/9781003147077-7
  17. Jain, Raksha; Bohra, Nitin; Singh, Rupesh Kumar; Upadhyay, Sudhir K.; Srivastava, Abhishek K.; Rajput, Vishnu D.. Autor correspondente: Singh, Rupesh Kumar. "Nanomaterials for Plants: From Ecophysiology to Signaling Mechanisms and Nutrient Uptake". In The Role of Nanoparticles in Plant Nutrition under Soil Pollution, 183-197. Springer International Publishing, 2022.
    Publicado • 10.1007/978-3-030-97389-6_8
  18. Singh, Shweta; Pradhan, Arunava; Singh, Rupesh Kumar; Singh, Abhishek; Minkina, Tatiana; Rajput, Vishnu D.. Autor correspondente: Singh, Rupesh Kumar. "Nanoparticles: Physiology, Chemistry, and Biochemistry". In The Role of Nanoparticles in Plant Nutrition under Soil Pollution, 21-42. Springer International Publishing, 2022.
    Publicado • 10.1007/978-3-030-97389-6_2
  19. Bohra, Nitin; Singh, Rupesh Kumar; Jain, Raksha; Sharma, Lav; Sathyanarayana, Eetela; Quiroz-Figueroa, Francisco Roberto; Rajput, Vishnu D.; et al. Autor correspondente: Singh, Rupesh Kumar. "Endophytic Bacteria for Plant Growth Promotion". In Bacterial Endophytes for Sustainable Agriculture and Environmental Management, 187-199. Springer Singapore, 2022.
    Publicado • 10.1007/978-981-16-4497-9_9
  20. Singh, Rupesh; Chokheli, V.; Rajput, V.; Dmitriev, P.; Varduny, T.; Minkina, T.; Singh, R.K.; Singh, A.. "Status and policies of GM crops in Russia". 2021.
    DOI: 10.1016/B978-0-12-820780-2.00003-0
  21. Singh, Shweta; Singh, Rupesh Kumar; Anandhan, Sivalingam; Priya, Jyoti; Jaiswal, Sandeep; Kumar, Ashok. Autor correspondente: Singh, Rupesh Kumar. "Advances in Genome Editing". In Genome Editing in Plants, 227-240. CRC Press, 2021.
    10.1201/9780367815370-16
  22. Jaiswal, Sandeep; Kumar, Ashok; Priya, Jyoti; Anandhan, Sivalingam; Singh, Shweta; Singh, Rupesh Kumar. Autor correspondente: Singh, Rupesh Kumar. "Genome Editing for Metabolic Engineering in Plants". In Genome Editing in Plants, 155-170. CRC Press, 2021.
    Publicado • 10.1201/9780367815370-11
  23. Singh, Rupesh; Singh, A.; Rajput, V.; Mehrotra, R.; Pal, N.; Singh, V.K.; Minkina, T.; et al. "Modern sustainable conservation practices for sustainable soil management". In Sustainable Soil Fertility Management. Nova Science Publishers, 2021.
    Publicado
  24. Singh, A.; Rajput, V.; Singh, A.K.; Sengar, R.S.; Singh, R.K.; Minkina, T.; Singh, Abhishek; et al. "Transformation Techniques and Their Role in Crop Improvements: A Global Scenario of GM Crops". In Policy Issues in Genetically Modified Crops, 515-542. Elsevier, 2020.
    10.1016/b978-0-12-820780-2.00023-6
  25. Sharma, Lav; Bohra, Nitin; Singh, Rupesh Kumar; Marques, Guilhermina. "Potential of Entomopathogenic Bacteria and Fungi". In Microbes for Sustainable Insect Pest Management, 115-149. Springer International Publishing, 2019.
    10.1007/978-3-030-23045-6_4
  26. Singh, Rupesh Kumar; Bohra, Nitin; Sharma, Lav; Anandhan, Sivalingam; Ruiz-May, Eliel; Quiroz-Figueroa, Francisco Roberto. Autor correspondente: Singh, Rupesh Kumar. "Inspection of Crop Wild Relative (Cicer microphyllum) as Potential Genetic Resource in Transgenic Development". In Advances in Plant Transgenics: Methods and Applications, 253-272. Springer Singapore, 2019.
    10.1007/978-981-13-9624-3_12
  27. Singh, Rupesh Kumar; Sharma, Lav; Bohra, Nitin; Anandhan, Sivalingam; Ruiz-May, Eliel; Quiroz-Figueroa, Francisco Roberto. Autor correspondente: Singh, Rupesh Kumar. "Recent Developments in Generation of Marker-Free Transgenic Plants". In Advances in Plant Transgenics: Methods and Applications, 127-142. Springer Singapore, 2019.
    10.1007/978-981-13-9624-3_6
  28. Singh, Rupesh Kumar; Bohra, Nitin; Sharma, Lav; Anandhan, Sivalingam; Ruiz-May, Eliel; Quiroz-Figueroa, Francisco Roberto. "Inspection of Crop Wild Relative (Cicer microphyllum) as Potential Genetic Resource in Transgenic Development". In Advances in Plant Transgenics: Methods and Applications, 253-272. Springer Singapore, 2019.
    10.1007/978-981-13-9624-3_12
  29. Singh, R.K.; Sharma, L.; Bohra, N.; Anandhan, S.; Ruiz-May, E.; Quiroz-Figueroa, F.R.. "Recent developments in generation of marker-free transgenic plants". 2019.
  30. Singh, R.K.; Bohra, N.; Sharma, L.; Anandhan, S.; Ruiz-May, E.; Quiroz-Figueroa, F.R.. "Inspection of crop wild relative (Cicer microphyllum) as potential genetic resource in transgenic development". 2019.
  31. Singh, Rupesh; Singh, R.K.; Hou, W.; Franklin, G.. "Construction of Hypericin Gland-Specific cDNA Library via Suppression Subtractive Hybridization.". 2016.
    10.1007/978-1-4939-3332-7_22
  32. Singh, R.K.; Ruiz-May, E.; García-Pérez, L.M.; Quiroz-Figueroa, F.R.; Singh, Rupesh Kumar; Ruiz-May, Eliel; García-Pérez, Luz María; Quiroz-Figueroa, Francisco R.. "Somatic embryogenesis in Jatropha curcas". In Somatic Embryogenesis: Fundamental Aspects and Applications, 401-412. Springer International Publishing, 2016.
    doi.org/10.1007/978-3-319-33705-0_22
Livro
  1. Singh, Rupesh. Composting of farm residues by nature-based solutions; A potential key towards sustainable agricultural practices. Estados Unidos: Springer One New York Plaza, Suite 4600 New York, NY 10004-1562, USA www.springer.com. 2025.
    Em revisão • Editor
  2. Singh, Rupesh. Nanotechnology Applications in Soil Science: From Remediation to Precision Agriculture. Estados Unidos: IGI Global. 2024.
    No prelo • Editor
  3. Singh, Rupesh. Cicer microphyllum, a wonder plant for abiotic stress management under cold arid zones. LAP Lambert Academic Publishing (2014). 2014.
    Publicado
Poster em conferência
  1. Singh, Rupesh. "Volatile fatty acids dynamics upon biochar, microbes and acidification treatments during storage of liquid fraction residue". Trabalho apresentado em IX Jornadas de Engenharia Agronómica, Department of Agronomy, UTAD, Vila Rea, Portugal, 2025.
  2. Singh, Rupesh. "FOREST DEBRIS-BASED BIOCHAR APPLICATION IN COMPOSTING PROCESS TO REDUCE GREENHOUSE GAS EMISSIONS: A NATURE BASED SUSTAINABLE SOLUTION". Trabalho apresentado em Agriculture for life, life for agriculture, 2024.
  3. Singh, Rupesh. "Chitosan modulate anthocyanin profile in vine-stems: potential use of vitiviniculture residues for industrial purpose.". Trabalho apresentado em ECO-BIO Dublin, Ireland., 2018.
  4. Singh, Rupesh. "In vitro grown shoot cuttings of Vitis vinifera L. cv. red grapes instigate secondary metabolites production upon Agrobacterium tumefaciens inoculation". Trabalho apresentado em ECO-BIO Dublin, Ireland, 2018.
  5. Singh, Rupesh. "Valorizing faba bean for animal feed supplements via biotechnological approach; suggestion paper". Trabalho apresentado em ECO-BIO Dublin, Ireland., 2018.
  6. Singh, Rupesh. "Metallothionin like gene from Cicer microphyllum is regulated by low-temperature stress". Trabalho apresentado em 5th World Congress of Cellular and Molecular Biology, Devi Ahilya Vishwavidyalaya, India, 2009.
  7. Singh, Rupesh. "Molecular cloning of cold-induced Inositol 1,4,5-trisphosphate kinase a key component for Ca2+ signal transduction". Trabalho apresentado em National Seminar on Frontiers in Biotechnology, Bharathiar University, Coimbatore, Tamilnadu, India, 2009.
  8. Singh, Rupesh. "Isolation of low temperature stress regulated genes from Cicer microphyllum by suppression subtraction hybridization". Trabalho apresentado em International conference on grain legumes: quality improvement, value addition and trade, 2009.
  9. Singh, Rupesh. "Cloning and in silico analysis of cold induced NAC transcription factor of Lepidium latifolium". Trabalho apresentado em International Conference on Current Trends in Biotechnology & Implications in Agriculture, Sardar Vallabh Bhai Patel University of Agriculture and Technology, Meerut, India, 2009.
Pré-impressão
  1. Lenar R Valiullin; Rishat S Mukhammadiev; Vladislav Egorov; Rinat S Mukhammadiev; Ainur I Yarullin; Vladimir V Sakhnov; Rupesh Kumar Singh; et al. "Microorganism Isolates Stimulate Forest Crop Growth and Protection". 2023. https://doi.org/10.20944/preprints202310.0926.v1.
    10.20944/preprints202310.0926.v1
Recurso online
  1. Singh, Rupesh. Agrobacterium-elicited Hypericum perforatum cell methanolic extract shows antibacterial activity against human pathogens. 2014. http://repositorium.sdum.uminho.pt/handle/1822/963 https://hdl.handle.net/1822/52445.
    DOI: 10.1055/s-0034-1394698
  2. Singh, Rupesh. Lignin and flavonoid content increases in Hypericum perforatum cell wall after Agrobacterium tumefaciens co-cultivation. 2014. DOI: 10.1055/s-0034-1394589.

Propriedade Intelectual

Patente
  1. RUPESH KUMAR SINGH; VIRGÍLIO ALEXANDRE CARDOSO E FALCODA COSTA; ANA ALEXANDRA RIBEIRO COUTINHO DEOLIVEIRA. 2022. "PROCESSO PARA AUMENTAR O TEOR DEANTOCIANINAS E DE POLIFENÓIS TOTAIS NO VINHO TINTO". Portugal.
    Concedida/Emitida
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2024/08/30 Synthesis and characterization of nano-biochar, interaction with emerging contaminants and application on organic matters to improve the nutrients recovery IHSS conference under the Session V: NOM and HS for environmental remediation
Italy (Rimini, Itália)
2024/07/19 Process to improve the qualitative characters in grapevine by sustainable application in vineyards workshop comparing Indian and Global agriculture education and research models
CAMBRIDGE INTERNATIONAL AGRICULTURE ORGANIZATION (Online, Índia)

Orientação

Título / Tema
Papel desempenhado 		Curso (Tipo)
Instituição / Organização
2024/05 - 2024/07 Estratégia de mitigação do efeito do lítio no solo com biochar e nanobiochar – um estudo de evitamento com Eisenia fetida
Coorientador de Paula Cristina Marinho Silva
 Licenciatura em Ciências do Ambiente (Licenciatura/Bacharelato)
Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
2024/05 - 2024/07 Efeito da Aplicação de Biochar e Nanobiochar na Mineralização de N a partir da matéria orgânica de um arenossolo
Coorientador de João Pedro Cerqueira Mourão
 Ciências do Ambiente (Licenciatura/Bacharelato)
Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
2024/05 - 2024/07 Efeito da aplicação de Biochar e nanoBiochar na emissão de gases com efeito de estufa a partir da matéria orgânica de um arenossolo
Coorientador
 Licenciatura em Ciências do Ambiente (Licenciatura/Bacharelato)
Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação) 		Instituição / Organização
2014/08/31 - 2014/09/04 Part of organization team of 62nd GA Meeting by Department of Biology, UMINHO, Braga (2014)
Conferência (Membro da Comissão Organizadora)

Participação em evento

Descrição da atividade
Tipo de evento 		Nome do evento
Instituição / Organização
2018/09/11 - 2018/09/20 FEBS Practical Course 2018 - Microspectroscopy: Functional Imaging of Biological Systems 11-20 September 2018 I Wageningen, Netherlands
Oficina (workshop)
 FEBS Practical Course 2018 - Microspectroscopy: Functional Imaging of Biological Systems
Wageningen Plant Research, Países Baixos
2018/03/05 - 2018/03/07 Training program VIII Hands-on Course in Sample Preparation using Nanoparticles for Proteomics
Oficina (workshop)
 VIII Hands-on Course in Sample Preparation using Nanoparticles for Proteomics
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/02/26 - 2018/02/28 Training program IX Hands-on Course in Ultrafast Sample Treatment for Proteomics
Oficina (workshop)
 IX Hands-on Course in Ultrafast Sample Treatment for Proteomics
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/04/01 - 2017/04/15 Training on advance proteomics; application of 2D Gel Electrophoresis for protein profiling
Outro
Instytut Genetyki Roslin Polskiej Akademii Nauk, Polónia
2003/08/05 - 2003/11/05 Short term project on fermentation technology at MOHAN GOLDWATER BREWERIES Ltd, India

Arbitragem científica em revista

Nome da revista (ISSN) Editora
2023/04 - Atual Special Issue "Plant Biotechnology: Applications in In Vitro Plant Conservation and Micropropagation" (2311-7524) MDPI
2023/03/01 - Atual Topical Advisory Panel Member of Sustainability (2071-1050) MDPI Basel
2024/09/10 - 2025/05/31 Agro-Environmental Nutrient Management Rooted in Nature-Based Solutions: Frontiers (2296-665X) Frontiers in Environmental Science
2022/10/20 - 2023/10/05 Sustainability, Special Issue "Exploring Wild Natural Diversity to Pick Out New Green Proxy Materials to Revamp Sustainable Agriculture" (ISSN 2071-1050) MDPI
2022/02/22 - 2023/02/22 Frontiers In Genomeediting: Genes at Work: Strategies for selection and development of Industrial Strains of Microbes and Plants Frontiers in genome editing

Comissão de avaliação

Descrição da atividade
Tipo de assessoria 		Instituição / Organização Entidade financiadora
2022/08/22 - Atual Invited by National Research and Development Agency (ANID), Government of Chille as external reviewer for proposals submitted to 2023 Competition for Initiation Into Research Fondecyt Projects.
Especialista

Entrevista (jornal / revista)

Descrição da atividade Jornal / Forum
2022/01/13 Interviewed by a French news magazine regarding my research of Chitosan application in Vineyards. The interview was published on 13-01-2022 in "Reussir VIGNE" N 292 entitled "Du chitosane sur les vignes pour davantage de polyphénols".

Expedição científica

Descrição da atividade Instituição / Organização
2024/09/01 - 2027/08/01 the project titled "Eco-Friendly Solutions for Heavy Metal Stabilization in Soil Using Biochar and Nanofertilizers for Barley Resilience: A Case Study for Achieving Sustainable Development Goals (SDGs) in Syunik Province" , I have agreed to be a Guest Professor for the scientific group. During the three-year project implementation, I will agree to stay at least 21 days at Faculty of Biology Yerevan State University Republic of Armenia to conduct activities related to the project, such as teaching.

Revisão ad hoc de artigos em revista

Nome da revista (ISSN) Editora
2022/04/04 - Atual Agriculture (2077-0472) MDPI (Basel, CH)
2022 - Atual Biology (ISSN 2079-7737) MDPI (Basel, CH)
2022 - Atual Environmental geochemistry and health Springer Nature (New York , US)
2022 - Atual Genes (2073-4425) MDPI (Basel, CH)
2022 - Atual Horticulturae (2311-7524) MDPI (Basel, CH)
2022 - Atual Life (2075-1729) MDPI
2022 - Atual Stresses (2673-7140) MDPI
2022 - Atual Toxins (ISSN 2072-6651) MDPI
2021/04/14 - Atual Applied Sciences ( ISSN: 2076-3417) MDPI (Basel, CH)
2021/02/02 - Atual Agronomy (2073-4395) MDPI (Basel, CH)
2021 - Atual Biological Research (ISSN: 0717-6287) Springer Nature (New York, US)
2021 - Atual Coatings (2079-6412) MDPI (Basel, CH)
2021 - Atual Environmental Sustainability (2523-8922) Springer Nature (New York, US)
2021 - Atual Foods (2304-8158) MDPI (Basel, CH)
2021 - Atual Forests (1999-4907) MDPI (Basel, CH)
2021 - Atual Metabolites (2218-1989) MDPI
2021 - Atual Microorganisms (2076-2607) MDPI
2021 - Atual Pathogens ((ISSN 2076-0817)) MDPI
2021 - Atual Physiology and Molecular Biology of Plants ( ISSN: 0971-5894) Springer Nature (New York, US)
2021 - Atual Water (ISSN 2073-4441) MDPI
2020 - Atual The International Journal of Molecular Sciences ( 1422-0067) MDPI (Basel, CH)
2020 - Atual Land (2073-445X) MDPI (Basel, CH)
2020 - Atual Molecules ((ISSN 1420-3049, COD) MDPI
2020 - Atual Plants (ISSN 2223-7747) MDPI
2020 - Atual Sustainability (ISSN 2071-1050) MDPI
2019 - Atual Nutrients ((ISSN 2072-6643)) MDPI

Tutoria

Tópico Nome do aluno
2014 - 2018 PhD in Molecular mechanism of Agrobactierium recognition and defense activation in recalcitrant plants WEINA HOU
Distinções

Prémio

2020 Finalist work for the ENOFORUM 2020 Award
2009 Best Poster Presentation