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Marlene Rafaela Cardoso Duarte Santos completed her Master's degree in Molecular Biology in Health at Escola Superior de Saúde Egas Moniz in 2015, following her Bachelor's in Marine Biology from Faculdade de Ciências e Tecnologia, Universidade do Algarve in 2013. Currently, she is pursuing her PhD in Veterinary Sciences at Faculdade de Medicina Veterinária, Universidade de Lisboa, starting from January 1, 2020. Her expertise lies in Biological Sciences with a particular emphasis on Molecular and Cell Biology, Structural Biology and Biochemistry. Published 7 articles in reputable journals. Engaged as a PhD Student Fellow in 1 project, Principal Investigator in 2 projects, and Research Fellow in 3 projects.
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Identification

Personal identification

Full name
Marlene Rafaela Cardoso Duarte Santos

Citation names

  • Duarte, Marlene

Author identifiers

Ciência ID
F712-6243-6678
ORCID iD
0000-0001-5634-699X
Scopus Author Id
57194048327

Email addresses

  • marlenesantos@fmv.ulisboa.pt (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Biology
  • Natural sciences - Biological Sciences - Biochemistry

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2020/01/01 - 2024
Ongoing
 Ciências Veterinárias (Doutoramento)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2013 - 2015
Concluded
 Biologia Molecular em Saúde (Mestrado)
Escola Superior de Saúde Egas Moniz, Portugal
"Envolvimento de variantes genéticas específicas na suscetibilidade para o risco aumentado de cancro do cólon e reto e na agressividade tumoral " (THESIS/DISSERTATION)
 17
2010 - 2013
Concluded
 Biologia Marinha (Licenciatura)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal
"n/a" (THESIS/DISSERTATION)
 15
Affiliation

Science

Category
Host institution 		Employer
2019/05/02 - 2019/12/31 Researcher (Research) Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2015/10/13 - 2018/02/18 Researcher (Research) Instituto Português de Oncologia de Lisboa Francisco Gentil EPE Unidade de Investigação em Patobiologia Molecular, Portugal
Instituto Português de Oncologia de Lisboa Francisco Gentil EPE Unidade de Investigação em Patobiologia Molecular, Portugal

Others

Category
Host institution 		Employer
2020/01 - 2024 PhD student Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2014/10/20 - 2016/09/18 Master's student Instituto Português de Oncologia de Lisboa Francisco Gentil EPE Unidade de Investigação em Patobiologia Molecular, Portugal
Projects

Grant

Designation Funders
2020/01/02 - 2024/09/30 New insights into bacterial cellulosome assembly
SFRH/BD/146965/2019
PhD Student Fellow
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019/05/02 - 2019/12/31 Molecular determinants of bacterial cellulosome diversity in different ecological niches
PTDC/BIA-MIC/5947/2014
Research Fellow
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2017/10/13 - 2018/02/18 T2C - Development of a novel delivery system (T2C) to Target metastatic colorectal cancer (mCRC)
BI_MESTRE_iNOVA-05_2017_1
Research Fellow
Instituto Português de Oncologia de Lisboa Francisco Gentil EPE Unidade de Investigação em Patobiologia Molecular, Portugal
 iNOVA4Health
Concluded
2016/09/19 - 2017/09/19 Molecular profiling and development of novel therapies to target mutated genes in aggressive thyroid and colorectal cancers
BI_MESTRE_iNOVA4Health_201601-02-017
Research Fellow
Instituto Português de Oncologia de Lisboa Francisco Gentil EPE Unidade de Investigação em Patobiologia Molecular, Portugal
 iNOVA4Health
Concluded

Contract

Designation Funders
2023/01/02 - 2023/10/31 Engineering a new highly specific protein purification system through structure-function studies on Ruminococcus flavefaciens cohesin-dockerin interactions
CONT PR34
Principal investigator
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal
 Universidade de Lisboa Faculdade de Medicina Veterinária
Concluded
2021/01/05 - 2021/10/31 Team work: the collaborative efforts of Ruminococcus flavefaciens and R. albus cellulosomes for fibre degradation in the rumen
CONTSet20 - 10
Principal investigator
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal
 Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal
Ongoing
Outputs

Publications

Journal article
  1. Duarte, Marlene; Carvalho, Ana Luísa; Ferreira, Magda C.; Caires, Beatriz; Romão, Maria João; Prates, José A.M.; Najmudin, Shabir; et al. "Tripartite binding mode of cohesin-dockerin complexes from Ruminococcus flavefaciens involving naturally truncated dockerins". Journal of Biological Chemistry (2025): https://doi.org/10.1016/j.jbc.2025.110325.
    Published • 10.1016/j.jbc.2025.110325
  2. Duarte M; Alves VD; Correia M; Caseiro C; Ferreira LMA; Romão MJ; Carvalho AL; et al. "Structure-function studies can improve binding affinity of cohesin-dockerin interactions for multi-protein assemblies.". International journal of biological macromolecules (2022): http://europepmc.org/abstract/med/36252630.
    10.1016/j.ijbiomac.2022.10.102
  3. Mónica M. Costa; Luís B. Pio; Pedro Bule; Vânia A. Cardoso; Marlene Duarte; Cristina M. Alfaia; Diogo F. Coelho; et al. "Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases". Animal Nutrition (2022): https://doi.org/10.1016/j.aninu.2022.01.004.
    10.1016/j.aninu.2022.01.004
  4. Duarte M; Viegas A; Alves VD; Prates JAM; Ferreira LMA; Najmudin S; Cabrita EJ; et al. "A dual cohesin-dockerin complex binding mode in Bacteroides cellulosolvens contributes to the size and complexity of its cellulosome.". The Journal of biological chemistry (2021): http://europepmc.org/abstract/med/33744293.
    10.1016/j.jbc.2021.100552
  5. Santos, Marlene. "37 – Methylation Changes At the Gnas Imprinted Locus in Pancreatic Cystic Neoplasms: Role in the Diagnosis of Mucinous and Malignant Cysts?". Gastroenterology (2019): http://dx.doi.org/10.1016/s0016-5085(19)36809-x.
    10.1016/s0016-5085(19)36809-x
  6. Pereira, C.V.; Duarte, M.; Silva, P.; da Silva, A.B.; Duarte, C.M.M.; Cifuentes, A.; García-Cañas, V.; et al. "Polymethoxylated flavones target cancer stemness and improve the antiproliferative effect of 5-fluorouracil in a 3D cell model of colorectal cancer". Nutrients 11 2 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85061145281&partnerID=MN8TOARS.
    10.3390/nu11020326
  7. Faias S; Duarte M; Albuquerque C; da Silva JP; Fonseca R; Roque R; Dias Pereira A; Chaves P; Cravo M. "Clinical Impact of KRAS and GNAS Analysis Added to CEA and Cytology in Pancreatic Cystic Fluid Obtained by EUS-FNA.". Digestive diseases and sciences (2018): http://europepmc.org/abstract/med/29796909.
    10.1007/s10620-018-5128-y
  8. Pereira LP; Silva P; Duarte M; Rodrigues L; Duarte CM; Albuquerque C; Serra AT. "Targeting Colorectal Cancer Proliferation, Stemness and Metastatic Potential Using Brassicaceae Extracts Enriched in Isothiocyanates: A 3D Cell Model-Based Study.". Nutrients (2017): http://europepmc.org/abstract/med/28394276.
    10.3390/nu9040368
Thesis / Dissertation
  1. Duarte, Marlene. "Envolvimento de variantes genéticas específicas na suscetibilidade para o risco aumentado do cancro do cólon e reto e na agressividade tumoral". Master, Escola Superior de Saúde Egas Moniz, 2015. http://hdl.handle.net/10400.26/9518.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/10 Ruminococcus flavefaciens cellulosome: a valuable tool for applied biotechnology Al4Animals - Comparative and Translational Medicine and Biotechnology
ICBAS - Instituto de Ciências Biomédicas Abel Salazar (Porto, Portugal)
2023/07/09 New insigths into Ruminococcus flavefaciens cellulosome assembly 21st European Carbohydrate Symposium
European Carbohydrate Organization (Paris, France)
2023/01/17 Unconventional assembly of ruminal cellulosomes 14th International Meeting of the Portuguese Carbohydrate Group – GLUPOR 14
Applied Molecular Biosciences Unit, NOVA School of Science and Technology (UCIBIO, FCT-NOVA) (Caparica, Portugal)
2016/04/28 Microsatellite instability-low and loss of heterozigozity in 2p may underlie increased susceptibility for familial rectal cancer 2nd ASPIC International Congress
IPO - Porto (Porto, Portugal)
Distinctions

Award

2019 Prémio CPP Melhor Trabalho sobre Patologia Pancreática
Clube Português do Pâncreas, Portugal