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I’ve obtained the PhD degree in Pharmacy (with specialization in Cell and Molecular Biology) in 2018 by the Faculty of Pharmacy of the University of Lisbon, the MSc in Biomedical Research in 2012 by the Faculty of Medicine of the University of Coimbra, and the BSc in Biomedical Science in 2010 by the Faculty of Sciences and Technology of the University of Algarve. I’m a Contracted Researcher in CIBB. So far, I’ve published 7 articles in journals, 1 book chapter and was awarded with 4 grants/prizes. During both my master and doctoral research I was focused on the study of cellular and molecular mechanisms associated with brain pathologies, namely gliomas and multiple sclerosis (MS). I have explored the developmental stage, from neural stem/progenitor cells to immature/early differentiated glia, which is more susceptible to malignant transformation. I’ve also studied the beneficial effect of some molecules that could be used as coadjuvants during temozolomide therapy in gliomas. During my PhD my scientific activity focused the role of S100B-RAGE axis in myelin-associated processes in pathological conditions. Studies included different models from in vitro primary cultures of differentiating oligodendrocytes–to evaluate changes throughout oligodendrogenesis–to ex vivo organotypic cerebellar slice cultures to evaluate de novo myelination, as well as demyelination and remyelination. Further studies included the in vivo model of MS, the EAE, where I’ve explored the expression of S100B-RAGE along the course of demyelination/remyelination, as well as the animal response to dimethyl fumarate–used in clinical practice for MS treatment. At the moment, I am investigating the role of mechanotransduction and its implications in OL differentiation/maturation, such as the mechanisms involved in biophysical signaling in OL under physiological and pathological conditions.
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Identification

Personal identification

Full name
Gisela Filipa Assunção Santos

Author identifiers

Ciência ID
EA1D-7443-50DD
ORCID iD
0000-0002-7264-9162

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
French Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
Education
Degree Classification
2013/04/01 - 2018/04/04
Concluded
 Farmácia (Doutoramento)
Major in Biologia Celular e Molecular
Universidade de Lisboa Faculdade de Farmácia, Portugal
"Deciphering the involvement of S100B-RAGE axis in inflammation-associated myelin damage " (THESIS/DISSERTATION)
 Distinção
2010/09/01 - 2012/11/12
Concluded
 Investigação Biomédica (Mestrado integrado)
Universidade de Coimbra Faculdade de Medicina, Portugal
"Establishing the validity of glycoursodeoxycholic acid as a coadjuvant of temozolomide therapy in gliomas" (THESIS/DISSERTATION)
 17
2007/09/01 - 2010/09/14
Concluded
 Ciências Biomédicas (Licenciatura)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal
"Animal Models of Retinal Neovascularization" (THESIS/DISSERTATION)
 13
Affiliation

Science

Category
Host institution 		Employer
2018/10/01 - 2021/03/31 Contracted Researcher (Research) Centro de Inovação em Biomedicina e Biotecnologia (CIBB), Portugal

Others

Category
Host institution 		Employer
2013/04/01 - 2018/04/04 PhD student FCT Universidade de Lisboa Faculdade de Farmácia, Portugal
2011/09/14 - 2012/09/27 MSc trainee Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
Projects

Grant

Designation Funders
2018/10/01 - Current BrEin-MS — Brain Elasticity in Multiple Sclerosis and implications in mechanomodulation of oligodendrocytes: a cellular and clinical approach
POCI-01-0145-FEDER-029516
Researcher
Fundação para a Ciência e a Tecnologia
Ongoing
2013/10 - 2014/09 Role of S100B in multiple sclerosis: from a biomarker to a therapeutic target
info:eu-repo/grantAgreement/FCT/SFRH/SFRH2%FBPD2%F967942%F2013/PT
grant.Provided by PTCRIS: 147348
Researcher
Secção Regional de Lisboa, Portuguese Pharmaceutical Society
Outputs

Publications

Book chapter
  1. Barateiro, A.; Santos, G.; Afonso, V.; Brites, D.; Fernandes, A.. "Role of S100B on oligodendrocyte physiology and pathology". In Oligodendrocytes: Biology, Functions and Role in the Pathology of Diseases, edited by Dana Swanson, 111-163. Nova Science Publishers, 2015.
    Published • 10.1002/9781634638722.ch5
Conference abstract
  1. Barros, Catarina; Pascoal, P; Santos, Gisela; Barateiro, Andreia; Freitas, Raquel; Brites, Dora; Graça, Luis; Fernandes, Adelaide. "Inhibition of S100B prevents ex vivo demyelination and improves EAE clinical score. Free Radical Biology and Medicine 120, S153". 2018.
    10.1016/j.freeradbiomed.2018.04.504
  2. Barros, Catarina; Pascoal, Pedro; Santos, Gisela; Barateiro, Andreia; Freitas, Raquel; Brites, Dora; Graça, Luis; Fernandes, Adelaide. "Targeting S100B reduces demyelination-associated pathogenesis in ex vivo and in vivo models mimicking multiple sclerosis. Multiple Sclerosis Journal 24, 604-604". 2018.
  3. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B-RAGE signalling affect de novo myelination and remyelination. Glia 65, E377-E378". 2017.
  4. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "Elevated levels of S100B impair oligodendrogenesis. Multiple Sclerosis Journal 21, 427-427". 2015.
  5. Santos, Gisela; Afonso, Vera; Barateiro, Andreia; Fernandes, Adelaide; Brites, Dora. "S100B modulates oligodendrocyte development process. Glia 63, T02-19B". 2015.
  6. Barateiro, Andreia; Ferreira, Carla; Afonso, Vera; Santos, Gisela; Cerqueira, JJ; Brites, Dora; Fernandes, Adelaide. "Multiple sclerosis: implications of higher S100B levels in demyelination and glia reactivity. Multiple Sclerosis Journal 21, 757-758". 2015.
Conference poster
  1. Santos, Gisela; Lourenço, Tânia; Grãos, Mário. "Mechanisms of mechano-modulation on oligodendrocytes differentiation". Paper presented in Advanced therapies in Neurodegeneration: Bridging Neuroscience, Bioengineering and Optogenetics, 2020.
  2. Santos, Gisela; Lourenço, Tânia; Grãos, Mário. "Mechano-modulation of events regulating oligodendrocyte differentiation". Paper presented in CIBB Meeting 2019, 2019.
  3. Barros, Catarina; Pascoal, Pedro; Santos, Gisela; Barateiro, Andreia; Freitas, Raquel; Brites, Dora; Graça, Luis; Fernandes, Adelaide. "Blockade of S100B prevents MS-associated pathogenesis in EAE model". Paper presented in 4th PhD Students Meeting of the Mind-Brain College of the Universidade de Lisboa, 2018.
  4. Barros, Catarina; Pascoal, Pedro; Santos, Gisela; Barateiro, Andreia; Freitas, Raquel; Brites, Dora; Graça, Luis; Fernandes, Adelaide. "Targeting S100B reduces demyelination-associated pathogenesis in ex vivo and in vivo models mimicking Multiple Sclerosis". Paper presented in 34th Congress of ECTRIMS, 2018.
  5. Barros, Catarina; Pascoal, Pedro; Santos, Gisela; Barateiro, Andreia; Freitas, Raquel; Brites, Dora; Graça, Luis; Fernandes, Adelaide. "S100B targeting prevents ex vivo demyelination and reduce EAE severity". Paper presented in 10th iMed.ULisboa Postgraduate Students’ Meeting & 3rd i3DU Meeting, 2018.
  6. Barros, Catarina; Pascoal, Pedro; Santos, Gisela; Barateiro, Andreia; Freitas, Raquel; Brites, Dora; Graca, Luis; Fernandes, Adelaide. "Inhibition of S100B prevents ex vivo demyelination and improves EAE clinical score". Paper presented in XX Congresso Nacional de Bioquímica/19th Society for Free Radical Research Biennial Meeting, 2018.
  7. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B and its receptor RAGE during de novo myelination and remyelination". Paper presented in XIII European Meeting on Glial Cell Function in Health and Disease, 2017.
  8. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B-RAGE axis modulates myelination, neuronal function and inflammatory milieu during neurodevelopment and after brain injury". Paper presented in 9th Postgraduate iMed.UL Students Meeting and 3rd i3DU Meeting, 2017.
  9. Pascoal, Pedro; Santos, Gisela; Barateiro, Andreia; Brites, Dora; Cerqueira, JJ; Fernandes, Adelaide. "S100B-RAGE axis is increased in Experimental Autoimmune Encephalomyelitis and reduced upon dimethyl fumarate treatment". Paper presented in XV Meeting of the Portuguese Society for Neurosciences, 2017.
  10. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B-RAGE axis as a regulator of oligodendrogenesis". Paper presented in XIX National Congress of Biochemistry, 2016.
  11. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B impairs oligodendrogenesis through its receptor RAGE". Paper presented in Ciência 2016. Encontro Com A Ciência E Tecnologia Em Portugal, 2016.
  12. Ferreira, Carla; Barateiro, Andreia; Santos, Gisela; Brites, Dora; Fernandes, Adelaide. "S100B mediates microglia reactivity in Multiple Sclerosis". Paper presented in 1st Meeting of the PhD students from the Mind-Brain College, 2015.
  13. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "Excessive S100B inhibits oligodendrocyte precursor migration and differentiation". Paper presented in 1st Meeting of the PhD students from the Mind-Brain College, 2015.
  14. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "Elevated levels of S100B impair oligodendrogenesis". Paper presented in 31st Congress of the ECTRIMS, 2015.
  15. Barateiro, Andreia; Afonso, Vera; Ferreira, Carla; Santos, Gisela; Cerqueira, JJ; Brites, Dora; van Horssen, Jack; Fernandes, Adelaide. "Multiple sclerosis: implications of high S100B levels in demyelination and glia reactivity". Paper presented in 31st Congress of the ECTRIMS, 2015.
  16. Ferreira, Carla; Barateiro, Andreia; Santos, Gisela; Brites, Dora; Fernandes, Adelaide. "Modulation of microglia reactivity by S100B in Multiple Sclerosis". Paper presented in 7th Postgraduate iMed.UL Students Meeting and 1st i3DU Meeting, 2015.
  17. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "Excessive S100B levels impair oligodendrogenesis and oligodendrocytes migration ability". Paper presented in 7th Postgraduate iMed.UL Students Meeting and 1st i3DU Meeting, 2015.
  18. Santos, Gisela; Afonso, Vera; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B modulates oligodendrocyte development process". Paper presented in XII European Meeting on Glial Cells in Health and Disease, 2015.
  19. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B affects oligodendrocyte differentiation and maturation processes". Paper presented in XIV Meeting of the Portuguese Society for Neurosciences, 2015.
  20. Barateiro, Andreia; Afonso, Vera; Santos, Gisela; Cerqueira, JJ; Brites, Dora; van Horssen, Jack; Fernandes, Adelaide. "S100B is a key player in multiple sclerosis pathology". Paper presented in XVIII Congress of the Portuguese Biochemical Society, 2014.
  21. Barateiro, Andreia; Afonso, Vera; Santos, Gisela; Cerqueira, JJ; Brites, Dora; van Horssen, Jack; Fernandes, Adelaide. "S100B levels are increased in multiple sclerosis and modulates demyelination, glial reactivity and inflammation". Paper presented in Joint ACTRIMS-ECTRIMS Meeting, 2014.
  22. Santos, Gisela; Afonso, Vera; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B in oligodendrocyte differentiation and maturation". Paper presented in 6th Postgraduate iMed.UL Students Meeting, 2014.
  23. Barateiro, Andreia; Santos, Gisela; Afonso, Vera; Brites, Dora; Fernandes, Adelaide. "In vitro models to study myelination, demyelination and remyelination". Paper presented in 5th Postgraduate iMed.UL Students Meeting, 2013.
  24. Santos, Gisela; Gomes, Cátia; Torrado, Ema; Falcão, Ana Sofia; Lopes, MC; Brites, Dora. "Combined use temozolomide with a multidrug-resistance protein 1 inhibitor sensitizes glioma cells". Paper presented in 4th Postgraduate iMed.UL Students Meetin, 2012.
  25. Santos, Gisela; Gomes, Cátia; Torrado, Ema; Falcão, Ana Sofia; Lopes, MC; Brites, Dora. "Multidrug Resistance- Associated Protein 1 Inhibition as a Way To Enhance Cytotoxicity of Temozolomide in Mouse Glioma Cells". Paper presented in 26a Reunião do Grupo de Estudo de Envelhecimento Cerebral e Demências (GEECD), 2012.
  26. Gomes, Cátia; Santos, Gisela; Torrado, Ema; Falcão, Ana Sofia; Brites, Dora. "Applying Neural Stem Cell Biology to Brain Tumor Research: New Cues For Gliomagenesis in the Elderly". Paper presented in 26a Reunião do Grupo de Estudo de Envelhecimento Cerebral e Demências (GEECD, 2012.
Journal article
  1. Santos, Gisela; Barateiro, Andreia; Brites, Dora; Fernandes, Adelaide. "S100B Impairs Oligodendrogenesis and Myelin Repair Following Demyelination Through RAGE Engagement". Frontiers in Cellular Neuroscience 14 (2020): http://dx.doi.org/10.3389/fncel.2020.00279.
    Published • 10.3389/fncel.2020.00279
  2. das Neves, Sofia P.; Santos, Gisela; Barros, Catarina; Pereira, Diana Rodrigues; Ferreira, Ricardo; Mota, Cristina; Monteiro, Susana; et al. "Enhanced cognitive performance in experimental autoimmune encephalomyelitis mice treated with dimethyl fumarate after the appearance of disease symptoms". Journal of Neuroimmunology 340 (2020): 577163. http://dx.doi.org/10.1016/j.jneuroim.2020.577163.
    Published • 10.1016/j.jneuroim.2020.577163
  3. Santos, Gisela; Barateiro, Andreia; Gomes, Cláudio M.; Brites, Dora; Fernandes, Adelaide. "Impaired oligodendrogenesis and myelination by elevated S100B levels during neurodevelopment". Neuropharmacology 129 (2018): 69-83. http://dx.doi.org/10.1016/j.neuropharm.2017.11.002.
    Published • 10.1016/j.neuropharm.2017.11.002
  4. Pinheiro, Rui; Braga, Cláudia; Santos, Gisela; Bronze, Maria R.; Perry, Maria J.; Moreira, Rui; Brites, Dora; Falcão, Ana S.. "Targeting Gliomas: Can a New Alkylating Hybrid Compound Make a Difference?". ACS Chemical Neuroscience 8 1 (2016): 50-59. http://dx.doi.org/10.1021/acschemneuro.6b00169.
    Published • 10.1021/acschemneuro.6b00169
  5. Gomes, Cátia; Santos, Gisela; Falcão, Ana Sofia; Brites, Dora. "Early Differentiating Mouse Astroglial Progenitors Share Common Protein Signatures with GL261 Glioma Cells". Journal of Stem Celll and Regenerative Biology 2 2 (2016): 1-15. http://dx.doi.org/10.15436/2471-0598.16.014.
    10.15436/2471-0598.16.014
  6. Barateiro, Andreia; Afonso, Vera; Santos, Gisela; Cerqueira, João José; Brites, Dora; van Horssen, Jack; Fernandes, Adelaide. "S100B as a Potential Biomarker and Therapeutic Target in Multiple Sclerosis". Molecular Neurobiology 53 6 (2015): 3976-3991. http://dx.doi.org/10.1007/s12035-015-9336-6.
    Published • 10.1007/s12035-015-9336-6
  7. Torrado, Ema F.; Gomes, Cátia; Santos, Gisela; Fernandes, Adelaide; Brites, Dora; Falcão, Ana S.. "Directing mouse embryonic neurosphere differentiation toward an enriched neuronal population". International Journal of Developmental Neuroscience 37 C (2014): 94-99. http://dx.doi.org/10.1016/j.ijdevneu.2014.07.001.
    Published • 10.1016/j.ijdevneu.2014.07.001
Thesis / Dissertation
  1. Santos, Gisela Filipa Assunção, 1988-. "Deciphering the involvement of S100B-rage axis in imflammation-associated myelin damage". PhD, 2018. http://hdl.handle.net/10451/34016.
  2. Santos, Gisela Filipa Assunção. "Establishing the validity of glycoursodeoxycholic acid as a coadjuvant of temozolomide therapy in gliomas". PhD, 2012. http://hdl.handle.net/10316/22700.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2020/01/02 Mechanomodulation of events regulating oligodendrocyte differentiation CIBB Neuroscience and Disease thematic line, Joint data clubs – a forum for discussion
CIBB (Coimbra, Portugal)
2018/02/05 Involvement of S100B-RAGE axis in myelin damage associated with inflammatory conditions: dysmyelination vs. demyelination XLVIII Reunião Anual da Sociedade Portuguesa de Farmacologia/XXXVI Reunião de Farmacologia Clínica/XVII Reunião de Toxicologia
(Lisboa, Portugal)
2017/03/09 S100B a new inflammatory target in Multiple Sclerosis 4th International Porto Congress of Multiple Sclerosis
(Porto, Portugal)
2016/11/28 S100B-RAGE interaction as a novel player in oligodendrogenesis 2nd Meeting of the PhD students from the Mind-Brain Doctoral College of the University of Lisbon
(Lisboa, Portugal)
2016/06/14 S100B and its receptor RAGE as new players in oligodendrogenesis 8th Postgraduate iMed.UL Students Meeting and 2nd i3DU Meeting
(Lisboa, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2020/09/01 - 2021/12/17 The role of Sp1 in oligodendrocyte differentiation: a mechanically regulated transcription factor Classification: 19
Supervisor of Miguel Ribeiro
 Biomedical Research (Master)
Universidade de Coimbra Faculdade de Medicina, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2016 - 2016 8th Postgraduate iMed.UL Students Meeting and 2nd i3DU Meeting (2016 - 2016)
Meeting (Member of the Organising Committee)
 Universidade de Lisboa Faculdade de Farmácia, Portugal
2015 - 2015 7th Postgraduate iMed.UL Students Meeting and 1nd i3DU Meeting (2015 - 2015)
Meeting (Member of the Organising Committee)
 Universidade de Lisboa Faculdade de Farmácia, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2014 - Current European Researchers' Night 2014-2016 and 2019
Exhibition
Universidade de Coimbra Museu da Ciência, Portugal

Universidade de Lisboa Museu Nacional de História Natural, Portugal
2012 - Current Science and Technology week
Workshop
Universidade de Coimbra, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal
2012 - Current Collaboration in “Brain awareness week” editions in 2014, 2015 and 2019, which intended to give information to high school students about research developed in neurosciences field. The laboratory was opened for visits.
Exhibition
Universidade de Coimbra, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal
2019 - 2019 Gestão de Resíduos Hospitalares Perigosos – Triagem e Acondicionamento
Seminar
Universidade de Coimbra, Portugal
2019 - 2019 "How to get published" provided by Elsevier
Seminar
Universidade de Coimbra, Portugal
2019 - 2019 II Modular Training in Laboratory Animal Science Functions A, B, D and special skills – according to the Directive 2010/63/EU”
Universidade de Coimbra, Portugal
2014 - 2017 Collaboration in “Scientific developed in vacations” from 2014 to 2017 editions, an initiative in collaboration with Ciência Viva and the Faculdade de Farmácia, University of Lisbon, where high school students were accepted to develop a mini project under the themes of the research developed in the laboratory.
Universidade de Lisboa Faculdade de Farmácia, Portugal
2016 - 2016 Statistics in Scientific Research
Workshop
Universidade de Lisboa Faculdade de Farmácia, Portugal
2015 - 2015 “Creating and Phenotyping Mouse models of Disease and Ageing”, organized by the COST Action MouseAGE at the MRC Harwell, member of the International Mouse Phenotyping Consortium (IMPC), Oxfordshire, United Kingdom
Workshop
2015 - 2015 “Introductory course of Glial cells” provided by the XII European Meeting on Glial Cell Function in Health and Disease committee, Bilbao, Spain.
Seminar
2015 - 2015 Design of Experiments in Pharmaceutical Sciences Research
Workshop
Universidade de Lisboa Faculdade de Farmácia, Portugal
2014 - 2014 Quantify what our brain tell us about our results - ImageJ Tutorial
Workshop
Universidade de Lisboa Faculdade de Farmácia, Portugal
2012 - 2012 "Culture of nervous cells: implementation and characterization" provided by the Neuron Glia Biology in Health and Disease Unit, iMed.ULisboa
Workshop
Universidade de Lisboa Faculdade de Farmácia, Portugal
2012 - 2012 Preparation of quality figures for publication
Workshop
Universidade de Lisboa Faculdade de Farmácia, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2020/12/17 Gene deletion and nanobodies strategies to target S100B and improve recovery in an ex vivo demyelinating model
(Thesis) Main arguer
 Alexandre Neto (Master)
2019/12/04 Determination of the transcription rate in human cells subjected to stimuli that modulate intracellular contractility
(Thesis) Main arguer
 Cláudio Alexandre Rocha Almeida (Other)

Committee member

Activity description
Role 		Institution / Organization
2014 - 2016 Member of the iMed.ULisbon Postgraduate Students Commission (ipSC). This commission is responsible for the organization of workshops to the scientific community of the Research Institute for Medicines (iMed.ULisbon) as well as an annual iMed.ULisbon Postgraduate Students Meeting.
Member
 iMed.ULisbon Postgraduate Students Commission (ipSC), Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2022 - Current Viruses MDPI
2021 - Current Cells MDPI
2019 - Current Neurochemical Research (1573-6903) Springer-Verlag

Mentoring / Tutoring

Topic Student name
2020/05/11 - 2020/05/29 Laboratory rotation: "Mechanical modulation of SP1 in oligodendrocyte differentiation" Miguel Ribeiro
2020/02/03 - 2020/02/21 Laboratory rotation: "SP1 transcript factor activity and MBP expression: the influence of substrate stiffness" Sara Cruz
2019/02 - 2019/04 Laboratory rotation: "Brain Elasticity in Multiple Sclerosis and implications in mechanomodulation of oligodendrocytes" Alexandre Neto
2016 - 2017 Collaboration in the laboratorial supervision of the Master’s thesis "S100B targeting to reduce demyelination and EAE pathogenesis" Pedro Pascoal
Distinctions

Award

2017 Best Oral Presentation in the 4th International Porto Congress of Multiple Sclerosis
Merck KGaA, Germany

Other distinction

2015 IBRO Stipend for the participation in the Introductory Course of the XII European Meeting on Glial Cells in Health and Disease
International Brain Research Organization, France
2015 COST-MouseAge grant for the participation in the MouseAGE Training School: Creating and Phenotyping Mouse models of Disease and Ageing
2013 PhD Fellowship (SFRH/BD/91437/2012)
Fundação para a Ciência e a Tecnologia, Portugal