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Identification

Personal identification

Full name
Maria Gabriela Machado de Almeida

Citation names

  • Almeida, Maria

Author identifiers

Ciência ID
DF16-FBB4-3564
ORCID iD
0000-0002-4508-7379
Education
Degree Classification
2003
Concluded
 Bioquímica (Doutoramento)
Major in Especialidade: Bioquímica Física
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Estudos Estruturais e Funcionais em Redutase de Nitrito" (THESIS/DISSERTATION)
1992/10/01 - 1996/09/01
Concluded
 Biotecnologia (Mestrado)
Major in Eng. Bioquímica
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Haloperoxidases de Vanádio da Alga Pelvetia canaliculata" (THESIS/DISSERTATION)
 Muito Bom
1986/10/01 - 1992/10/01
Concluded
 Química (Licenciatura)
Major in Científico
Universidade de Lisboa Faculdade de Ciências, Portugal
 15
Affiliation

Science

2014/01/01 - Current Principal Investigator (Research)
Centro de Investigação Interdisciplinar Egas Moniz, Portugal
2003/11/01 - Current Principal Investigator (Research)
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2008/10/01 - 2012/09/30 Auxiliary Researcher (Research)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
1993/05/02 - 1995/04/30 Research Trainee (Research)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Teaching in Higher Education

2003/11/01 - Current Associate Professor (University Teacher)
Egas Moniz Cooperativa de Ensino Superior CRL, Portugal
2013 - 2016 Teacher Coordinator (Polytechnic Teacher)
Escola Superior de Saúde Egas Moniz, Portugal
2013 - 2016 Associate Professor (University Teacher)
Instituto Superior de Ciências da Saúde Egas Moniz, Portugal
2005/07/01 - 2005/07/31 Visiting Professor (University Teacher)
Aix-Marseille Université, France
1996/03/01 - 1997/07/01 Assistente Convidado (University Teacher)
Universidade do Algarve - Campus de Gambelas, Portugal

Others

2019/08/05 - Current Empreendorismo
Nitrogen Sensing Solutions Lda, Portugal
Projects

Grant

Designation Funders
1997 - 2001/09/30 Structural and Functional Studies on a Nitrite Reductase
PRAXIS XXI/BD/11349/97
PhD Student Fellow
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
 Fundação para a Ciência e a Tecnologia
1993 - 1995/04/30 AMINO ACID COMPOSITION AND PROTEIN SEQUENCING
ef. F12 – Proc. 1691
Research Technician Fellow

Contract

Designation Funders
2019 - Current NOX SENSING: MEASURING NITRIC OXIDES METABOLITES IN PHYSIOLOGICAL SAMPLES
NO2Probe
Principal investigator
Centro de Investigação Interdisciplinar Egas Moniz, Portugal
 Centro de Investigação Interdisciplinar Egas Moniz
2018 - 2018/12/01 PROTEOMYCETES - NOVEL CHEMBIOCHEMICAL PROPERTIES OF MARINE ACTINOMYCETES STREPTOMYCES ACULEOLATUSSPP. UNVEILED BY A COMBINED PROTEOMIC AND METABOLOMIC APPROACH
PROTEOMYCETES
Principal investigator
Centro de Investigação Interdisciplinar Egas Moniz, Portugal
 Centro de Investigação Interdisciplinar Egas Moniz
2014 - 2015/06/30 POINT OF CARE TESTING OF CARDIOVASCULAR DISEASES MARKERS – DETECTION OF HOMOCYSTEIN-THIOLACTONE.
EXPL/DTP-PIC/1758/2013
Principal investigator
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
 Fundação para a Ciência e a Tecnologia
2012 - 2014/03/31 PROBING THE REGULATION MECHANISM OF CYTOCHROME CD1 NITRITE REDUCTASE - A COMBINED ELECTROCHEMICAL AND CRYSTALLOGRAPHIC APPROACH
A-38/12
Principal investigator
REQUIMTE, Portugal
 CRUP - Conselho de Reitores das Universidades Portuguesas

GABINETE DE RELAÇÕES INTERNACIONAIS DA CIÊNCIA E DO ENSINO SUPERIOR
2005 - 2010/10/31 DEVELOPMENT OF ENZYME BIOSENSORS FOR MULTIPARAMETRIC CONTROL AND MONITORING OF ENVIRONMENTAL SAMPLES.
POCI/QUI/58026/2004
PPCDT/QUI/58026/2004
Principal investigator
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
 Fundação para a Ciência e a Tecnologia
2004 - 2007/03/31 DEVELOPMENT OF ELECTROCHEMICAL BIOSENSORS FOR ON-LINE DETERMINATION OF NITRITE AND NITRATE IN COMPLEX SAMPLES.
Not applicable
Principal investigator
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
 REQUIMTE
2005 - 2006/10/31 NANOEMSAMBLED BASED ELECTROACTIVE BIOINTERFACES FOR ENVIRONMENTAL AND FOOD ANALYSIS OF NITRATES AND NITRITES
Project 18244
Principal investigator
REQUIMTE, Portugal
 GABINETE DE RELAÇÕES INTERNACIONAIS DA CIÊNCIA E DO ENSINO SUPERIOR
2005 - 2006/09/30 STUDY OF A MULTIHEMIC NITRITE REDUCTASE BY DIRECT ELECTROCHEMISTRY AND ELECTRONIC PARAMAGNETIC RESSONANCE SPECTROSCOPY
F-11/05
Principal investigator
REQUIMTE, Portugal
 CRUP - Conselho de Reitores das Universidades Portuguesas
Outputs

Publications

Conference abstract
  1. Moura, I; Cabrito, I; Almeida, G; Cunha, C; Romao, MJ; Moura, JJG. 2003. "Molecular aspects of denitrification/nitrate dissimilation". 195-195.
    10.1016/S0162-0134(03)80717-1
  2. Almeida, G; Tavares, P; Lampreia, J; Moura, JJG; Moura, I. 2001. "Development of an electrochemical biosensor for nitrite determination". 121-121.
  3. Almeida, G; Lampreia, J; Moura, JJG; Moura, I. 1999. "New biochemical studies on nitrite reductase from Desulfovibrio desulfuricans ATCC 27774". 63-63.
  4. Almeida, M.; Almeida, M. G.; Humanes, M.; Melo, R. A.; Silva, J. A.; Frausto da Silva, J. J. R.. 1997. "Novel vanadium-dependent haloperoxidases in brown algae (Fucaceae and Laminariaceae) from Portugal". 1-2.
Conference paper
  1. Almeida, Maria. 2018. "Translational Research and Innovation in Human and Health Science".
    10.1080/07853890.2018.1427445
  2. Almeida, Maria. 2015. "Mitoxantrone elicits oxidative stress and proteomic changes in HL-1 cardiomyocytes at clinically relevant doses".
    10.1016/j.toxlet.2015.08.771
  3. Manolis, Matzapetakis; M. Gabriela Almeida; Sofia, R. Pauleta; Célia, M. Silveira. 2012. "Copper tolerance in Marinobacter hydrocarbonoclasticus. Proteomic analysis of the periplasm". 608-612.
    :10.1142/9789814405041_0124
Journal article
  1. Almeida, Maria. 2019. "Star-Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry: the Case Study of Cytochrome c". ChemElectroChem. http://dx.doi.org/10.1002/celc.201901393.
    10.1002/celc.201901393
  2. Almeida, Maria. 2019. "Characterization of Plasmonic Effects in AuNP+rGO Composite as a Sensing Layer for a Low-cost Lab-on-chip Biosensor". 2019 PhotonIcs & Electromagnetics Research Symposium - Spring (PIERS-Spring). http://dx.doi.org/10.1109/piers-spring46901.2019.9017299.
    10.1109/piers-spring46901.2019.9017299
  3. Monteiro, Tiago; Macedo, Maria dos Anjos; Almeida, Maria Gabriela. 2019. "Voltammetric appraisal of PON1’s activities: a preliminary study". Annals of Medicine 51 (sup1): 35-35. http://dx.doi.org/10.1080/07853890.2018.1560078.
    10.1080/07853890.2018.1560078
  4. Fernandes, Ana F.; Costa, Lígia; Sousa, Joana R.; Zalocha, Justyna; Grilo, Inês R.; Sobral, Rita G.; Gaudêncio, Susana P.; Gabriela Almeida, M.. 2019. "Biological activities of marine-derived actinomycetes: testing the aqueous extracellular phase of Streptomyces aculeolatus". Annals of Medicine 51 (sup1): 44-44. http://dx.doi.org/10.1080/07853890.2018.1561899.
    10.1080/07853890.2018.1561899
  5. Almeida, Maria. 2019. "Saccharomycin, a biocide from S. cerevisiae that kill-off other yeasts". Annals of Medicine. http://dx.doi.org/10.1080/07853890.2018.1562694.
    10.1080/07853890.2018.1562694
  6. Tiago Monteiro; Maria Gabriela Almeida. 2019. "Electrochemical Enzyme Biosensors Revisited: Old Solutions for New Problems". Critical Reviews in Analytical Chemistry: 1-23. https://doi.org/10.1080/10408347.2018.1461552.
    10.1080/10408347.2018.1461552
  7. Monteiro, Tiago; Gomes, Sara; Jubete, Elena; Añorga, Larraitz; Silveira, Célia M.; Almeida, Maria Gabriela. 2019. "A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide". Scientific Reports 9 (1): 2622-2622. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000459399400091&KeyUID=WOS:000459399400091.
    10.1038/s41598-019-39209-y
  8. Almeida, Maria. 2017. "Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate - biosynthetic costs modulate substrate selection.". Biochimica et biophysica acta. http://europepmc.org/abstract/med/28847524.
    10.1016/j.bbapap.2017.07.021
  9. Branco P; Kemsawasd V; Santos L; Diniz M; Caldeira J; Almeida MG; Arneborg N; Albergaria H. 2017. "Saccharomyces cerevisiae accumulates GAPDH-derived peptides on its cell surface that induce death of non-Saccharomyces yeasts by cell-to-cell contact.". http://europepmc.org/abstract/med/28449125.
    10.1093/femsec/fix055
  10. Almeida, Maria. 2016. "Biosensing with Paper-Based Miniaturized Printed Electrodes–A Modern Trend". Biosensors. http://www.mdpi.com/2079-6374/6/4/51.
    10.3390/bios6040051
  11. Humberto A. Pedroso; Célia M. Silveira; Rui M. Almeida; Ana Almeida; Stéphane Besson; Isabel Moura; José J.G. Moura; M. Gabriela Almeida. 2016. "Electron transfer and docking between cytochrome cd 1 nitrite reductase and different redox partners — A comparative study". Biochimica et Biophysica Acta (BBA) - Bioenergetics 1857 (9): 1412-1421. https://doi.org/10.1016/j.bbabio.2016.04.279.
    10.1016/j.bbabio.2016.04.279
  12. Branco P; Francisco D; Monteiro M; Almeida MG; Caldeira J; Arneborg N; Prista C; Albergaria H. 2016. "Antimicrobial properties and death-inducing mechanisms of saccharomycin, a biocide secreted by Saccharomyces cerevisiae.". http://europepmc.org/abstract/med/27502415.
  13. Almeida, Maria. 2015. "Synthesis of WO3 Nanoparticles for Biosensing Applications". Sensors Actuactors B. http://www.sciencedirect.com/science/article/pii/S0925400515303543.
    10.1016/j.snb.2015.09.046
  14. Almeida, Maria. 2015. "Cell-to-cell contact and antimicrobial peptides play a combined role in the death of Lachanchea thermotolerans during mixed-culture alcoholic fermentation with Saccharomyces cerevisiae.". FEMS Microbiology Letters. http://femsle.oxfordjournals.org/content/362/14/fnv103.
  15. Nair, Rashmi R.; Silveira, Celia M.; Diniz, Mario S.; Almeida, Maria G.; Moura, Jose J. G.; Rivas, Maria G.. 2015. "Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess". Journal of Biological Inorganic Chemistry 20 (2): 311-322. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000350236100013&KeyUID=WOS:000350236100013.
    10.1007/s00775-014-1224-4
  16. Silveira, Celia M; Quintas, Pedro O; Moura, Isabel; Moura, Jose J G; Hildebrandt, Peter; Almeida, M Gabriela; Todorovic, Smilja. 2015. "SERR Spectroelectrochemical Study of Cytochrome cd1 Nitrite Reductase Co-Immobilized with Physiological Redox Partner Cytochrome c552 on Biocompatible Metal Electrodes.". PloS one 10 (6): e0129940-e0129940. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:26091174&KeyUID=MEDLINE:26091174.
    10.1371/journal.pone.0129940
  17. Monteiro, Tiago; Rodrigues, Patricia R; Goncalves, Ana Luisa; Moura, Jose J G; Jubete, Elena; Anorga, Larraitz; Piknova, Barbora; et al. 2015. "Construction of effective disposable biosensors for point of care testing of nitrite.". Talanta 142: 246-51. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:26003719&KeyUID=MEDLINE:26003719.
    10.1016/j.talanta.2015.04.057
  18. Branco, Patricia; Francisco, Diana; Chambon, Christophe; Hebraud, Michel; Arneborg, Nils; Almeida, Maria Gabriela; Caldeira, Jorge; et al. 2014. "Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactions". Applied Microbiology and Biotechnology 98 (2): 843-853. http://hdl.handle.net/10400.26/18446.
    10.1007/s00253-013-5411-y
  19. Dias, Clara; Marinho, Aline; Morello, Judit; Almeida, Gabriela; Caixas, Umbelina; Soto, Karina; Monteiro, Emilia; Pereira, Sofia. 2014. "Monitoring of the lactonase activity of paraoxonase-1 enzyme in HIV-1-infection.". Journal of the International AIDS Society 17 (4 Suppl 3): 19682-19682.
    10.7448/IAS.17.4.19682
  20. Silveira, C.M.; Pimpão, M.; Pedroso, H.A.; Rodrigues, P.R.S.; Moura, J.J.G.; Pereira, M.F.R.; Almeida, M.G.. 2013. "Probing the surface chemistry of different oxidized MWCNT for the improved electrical wiring of cytochrome c nitrite reductase". Electrochemistry Communications 35: 17-21. http://www.scopus.com/inward/record.url?eid=2-s2.0-84881526846&partnerID=MN8TOARS.
    10.1016/j.elecom.2013.07.027
  21. Silveira, C.M.; Almeida, M.G.. 2013. "Small electron-transfer proteins as mediators in enzymatic electrochemical biosensors". Analytical and Bioanalytical Chemistry 405 (11): 3619-3635. http://www.scopus.com/inward/record.url?eid=2-s2.0-84877114970&partnerID=MN8TOARS.
    10.1007/s00216-013-6786-4
  22. Rodrigues, M.; Correia, C.; Silveira, C.M.; Moura, J.J.G.; Ochoteco, E.; Jubete, E.; Almeida, M.G.. 2012. "Nitrite biosensing with disposable electrode strips: A preliminary study". BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices: 32-36. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861967204&partnerID=MN8TOARS.
  23. Serra, A.S.; Jorge, S.R.; Silveira, C.M.; Moura, J.J.G.; Jubete, E.; Ochoteco, E.; Cabañero, G.; Grande, H.; Almeida, M.G.. 2011. "Cooperative use of cytochrome cd1 nitrite reductase and its redox partner cytochrome c 552 to improve the selectivity of nitrite biosensing". Analytica Chimica Acta 693 (1-2): 41-46. http://www.scopus.com/inward/record.url?eid=2-s2.0-79954787783&partnerID=MN8TOARS.
    10.1016/j.aca.2011.03.029
  24. Almeida, M.G.; Serra, A.; Silveira, C.M.; Moura, J.J.. 2010. "Nitrite biosensing via selective enzymes--a long but promising route.". Sensors (Basel, Switzerland) 10 (12): 11530-11555. http://www.scopus.com/inward/record.url?eid=2-s2.0-79959797858&partnerID=MN8TOARS.
    10.3390/s101211530
  25. Silveira, C.M.; Baur, J.; Holzinger, M.; Moura, J.J.G.; Cosnier, S.; Almeida, M.G.. 2010. "Enhanced Direct Electron Transfer of a Multihemic Nitrite Reductase on Single-walled Carbon Nanotube Modified Electrodes". Electroanalysis 22 (24): 2973-2978. http://www.scopus.com/inward/record.url?eid=2-s2.0-78650201908&partnerID=MN8TOARS.
    10.1002/elan.201000363
  26. Almeida, M.G.; Silveira, C.M.; Besson, S.; Moura, I.; Moura, J.J.G.. 2010. "Measuring the cytochrome c nitrite reductase activitypractical considerations on the enzyme assays". Bioinorganic Chemistry and Applications 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955363747&partnerID=MN8TOARS.
    10.1155/2010/634597
  27. Silveira, C.M.; Gomes, S.P.; Araújo, A.N.; Montenegro, M.C.B.S.M.; Todorovic, S.; Viana, A.S.; Silva, R.J.C.; Moura, J.J.G.; Almeida, M.G.. 2010. "An efficient non-mediated amperometric biosensor for nitrite determination". Biosensors and Bioelectronics 25 (9): 2026-2032. http://www.scopus.com/inward/record.url?eid=2-s2.0-77951623810&partnerID=MN8TOARS.
    10.1016/j.bios.2010.01.031
  28. Zhang, Z.; Xia, S.; Leonard, D.; Jaffrezic-Renault, N.; Zhang, J.; Bessueille, F.; Goepfert, Y.; et al. 2009. "A novel nitrite biosensor based on conductometric electrode modified with cytochrome c nitrite reductase composite membrane". Biosensors and Bioelectronics 24 (6): 1574-1579. http://www.scopus.com/inward/record.url?eid=2-s2.0-68349118562&partnerID=MN8TOARS.
    10.1016/j.bios.2008.08.010
  29. Almeida, M. Gabriela; Silveira, Célia M.; Moura, José J.G.; Almeida, M.G.; Silveira, C.M.; Moura, J.J.G.. 2007. "Biosensing nitrite using the system nitrite redutase/Nafion/methyl viologen—A voltammetric study". Biosensors and Bioelectronics 22 (11): 2485-2492. http://dx.doi.org/10.1016/j.bios.2006.09.027.
    10.1016/j.bios.2006.09.027
  30. Almeida, M.G.; Silveira, C.M.; Guigliarelli, B.; Bertrand, P.; Moura, J.J.G.; Moura, I.; Léger, C.. 2007. "A needle in a haystack: The active site of the membrane-bound complex cytochrome c nitrite reductase". FEBS Letters 581 (2): 284-288. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846273112&partnerID=MN8TOARS.
    10.1016/j.febslet.2006.12.023
  31. Gomes, S.P.; Odložilíková, M.; Gabriela Almeida, M.; Araújo, A.N.; Couto, C.M.C.M.; Montenegro, M.C.B.S.M.. 2007. "Application of lactate amperometric sol-gel biosensor to sequential injection determination of l-lactate". Journal of Pharmaceutical and Biomedical Analysis 43 (4): 1376-1381. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847186319&partnerID=MN8TOARS.
    10.1016/j.jpba.2006.11.027
  32. Chen, H.; Mousty, C.; Cosnier, S.; Silveira, C.; Moura, J.J.G.; Almeida, M.G.. 2007. "Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH". Electrochemistry Communications 9 (9): 2240-2245. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548019988&partnerID=MN8TOARS.
    10.1016/j.elecom.2007.05.030
  33. Da Silva, S.; Cosnier, S.; Almeida, M.G.; Moura, J.J.G.. 2004. "An efficient poly(pyrrole-viologen)-nitrite reductase biosensor for the mediated detection of nitrite". Electrochemistry Communications 6 (4): 404-408. http://www.scopus.com/inward/record.url?eid=2-s2.0-1642359212&partnerID=MN8TOARS.
    10.1016/j.elecom.2004.02.007
  34. Cunha, C.A.; Macieira, S.; Dias, J.M.; Almeida, G.; Gonçalves, L.L.; Costa, C.; Lampreia, J.; et al. 2003. "Cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA)". Journal of Biological Chemistry 278 (19): 17455-17465. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037592981&partnerID=MN8TOARS.
    10.1074/jbc.M211777200
  35. Almeida, M.G.; Macieira, S.; Gonçalves, L.L.; Huber, R.; Cunha, C.A.; Romão, M.J.; Costa, C.; et al. 2003. "The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774. Re-evaluation of the spectroscopic data and redox properties". European Journal of Biochemistry 270 (19): 3904-3915. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141704108&partnerID=MN8TOARS.
    10.1046/j.1432-1033.2003.03772.x
  36. Goncalves, LML; Cunha, C; Almeida, G; Macieira, S; Costa, C; Lampreia, J; Romao, MJ; Moura, JJG; Moura, I. 2001. "Structural studies on Desulfovibrio desulfuricans ATCC 27774 multiheme nitrite reductase - characterization of the subunits". Journal of Inorganic Biochemistry 86 (1): 316-316. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000170467300603&KeyUID=WOS:000170467300603.
  37. Dias, J.M.; Cunha, C.A.; Teixeira, S.; Almeida, G.; Costa, C.; Lampreia, J.; Moura, J.J.G.; Moura, I.; Romão, M.J.. 2000. "Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774". Acta Crystallographica Section D: Biological Crystallography 56 (2): 215-217. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034141953&partnerID=MN8TOARS.
    10.1107/S0907444999016066
  38. Girotti, S.; Ferri, E.N.; Fini, F.; Ruffini, F.; Budini, R.; Moura, I.; Almeida, G.; et al. 1999. "Enzymatic spectrophotometric determination of nitrites in beer". Analytical Letters 32 (11): 2217-2227. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032767295&partnerID=MN8TOARS.
Thesis / Dissertation
  1. Serrano, Daniela Filipa Tavares. 2017. "Identificação de alterações no perfil proteómico de uma estirpe industrial mutante, Saccharomyces cerevisiae PYCC 5334, capaz de consumo simultâneo de glucose e maltose". Mestrado. http://hdl.handle.net/10362/27974.
  2. Gomes, Sara Isabel Palhas. 2017. "Imobilização de enzimas em matrizes de sol-gel: novos desenvolvimentos em biossensores de nitrito e glucose". Mestrado. http://hdl.handle.net/10362/24082.
  3. Guanilho, Mariana Mendes Perestrelo. 2017. "O óxido nítrico e a doença periodontal". Mestrado. http://hdl.handle.net/10400.26/19990.
  4. Leite, Brigite Alícia Camões de Moura. 2017. "Avaliação da composição química dos cones de gutta-percha utilizados atualmente no tratamento endodôntico". Mestrado. http://hdl.handle.net/10400.26/20000.
  5. Crespo, Ana Patrícia Mouta. 2016. "Desenvolvimento de eléctrodos baseados em nanopartículas de WO3 para aplicação como biossensor". Mestrado. http://hdl.handle.net/10362/20616.
  6. Neves, Nuno de Sousa Paulino. 2016. "The role of human paraoxonase 1 (PON-1) in viral infections". Mestrado. http://hdl.handle.net/10400.26/17678.
  7. Glória, Luís Miguel Lucas. 2016. "Homocisteinilação de proteínas na aterosclerose". Mestrado. http://hdl.handle.net/10400.26/17680.
  8. Santos, Lara Andreia Brito dos. 2015. "Purification of antimicrobial peptides secreted by Saccharomyces cerevisiae and proteomic analysis of cell membrane-associated proteins". Mestrado. http://hdl.handle.net/10400.26/11109.
  9. Oliveira, Francisco Sousa de. 2015. "Desenvolvimento de novas ferramentas analíticas para a monitorização da actividade da Paraoxonase 1 em amostras de plasma humano". Mestrado. http://hdl.handle.net/10362/15444.
  10. Cardoso, Nuno Maria Lago Ferreira da Veiga. 2015. "Theranostics: novas tendências em medicina personalizada". Mestrado. http://hdl.handle.net/10400.26/10950.
  11. Mendes, Tatiana Soraia dos Santos. 2015. "Diagnóstico e tratamento da hiperhomocisteinémia no contexto das doenças cardiovasculares". Mestrado. http://hdl.handle.net/10400.26/11334.
  12. Sousa, Joana Rita Lourenço. 2014. "Proteómica microbiana: análise comparativa da bactéria redutora de sulfato Desulfovibrio desulfuricans ATCC 27774 e das actinobactérias Salinispora arenicola e Salinispora pacifica". Mestrado. http://hdl.handle.net/10362/14220.
  13. Pedroso, Humberto Agostinho. 2013. "Características estruturais e mecanísticas da enzima desnitrificante Redutase do Nitrito Citocromo cd1". Mestrado. http://hdl.handle.net/10362/10898.
  14. Rodrigues, Patrícia Raquel dos Santos. 2013. "Development of oxidoreductase based electrochemical biosensors". Mestrado. http://hdl.handle.net/10362/10427.
  15. Serra, Alexandra Sofia Vasques Gonçalves. 2012. "Isolamento e caracterização de enzimas multihémicas de origem microbiana e sua aplicação no desenvolvimento de biossensores". Doutoramento. http://hdl.handle.net/10362/7627.
  16. Silveira, Célia Marisa Costa Figueiredo. 2011. "Development of electrochemical nitrite biosensors using cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774". Doutoramento. http://hdl.handle.net/10362/6811.
  17. Fonseca, Fernando Manuel de Sousa Alves da. 2010. "Bateria recarregável com ânodo de alumínio". Mestrado. http://hdl.handle.net/10362/5885.

Other

Other output
  1. Pedroso, Humberto A.; Silveira, Célia M.; Almeida, Rui M.; Almeida, Ana; Besson, Stéphane; Moura, Isabel; Moura, José J G; et al. 2016. Electron transfer and docking between cytochrome cd1 nitrite reductase and different redox partners - A comparative study. Cytochrome cd1 nitrite reductases (cd1NiRs) catalyze the reduction of nitrite to nitric oxide in denitrifying bacteria, such as Marinobacter hydrocarbonoclasticus. Previous work demonstrated that the enzymatic activity depends on a structural pre-activation triggered by the entry of electrons through the electron transfer (ET) domain, which houses a heme c center. The catalytic activity of M. hydr. http://www.scopus.com/inward/record.url?scp=84974603879&partnerID=8YFLogxK.
    https://doi.org/10.1016/j.bbabio.2016.04.279
  2. Kemsawasd, Varongsiri; Branco, Patrícia; Almeida, Maria Gabriela; Caldeira, Jorge; Albergaria, Helena Albergaria; Arneborg, Nils. 2015. Cell-to-cell contact and antimicrobial peptides play a combined role in the death of Lachanchea thermotolerans during mixed-culture alcoholic fermentation with Saccharomyces cerevisiae. The roles of cell-to-cell contact and antimicrobial peptides in the early death of Lachanchea thermotolerans CBS2803 during anaerobic, mixed-culture fermentations with Saccharomyces cerevisiae S101 were investigated using a commercially available, double-compartment fermentation system separated by cellulose membranes with different pore sizes, i.e. 1000 kDa for mixed- and single-culture fermentat. http://hdl.handle.net/10400.26/18447.
    FEMS Microbiol Lett (2015) 362 (14): fnv103. DOI 10.1093/femsle/fnv103
  3. Almeida, Maria Gabriela; Serra, Alexandra; Silveira, Célia M.; Moura, José J. G.. 2010. Nitrite biosensing via selective enzymes—a long but promising route. The last decades have witnessed a steady increase of the social and political awareness for the need of monitoring and controlling environmental and industrial processes. In the case of nitrite ion, due to its potential toxicity for human health, the European Union has recently implemented a number of rules to restrict its level in drinking waters and food products. Although several analytical pro. http://hdl.handle.net/10362/6972.
  4. Silveira, Célia M.; Besson, Stéphane; Moura, Isabel; Moura, José J. G.; Almeida, Maria Gabriela. 2010. Measuring the cytochrome c nitrite reductase activity—practical considerations on the enzyme assays. The cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction. Although extensively characterized from the spectroscopic and structural points-of-view, some of its kinetic aspects are still under explored. In this work the kinetic behaviour of ccNiR has been evaluated in a systematic manner using two. http://hdl.handle.net/10362/6970.
  5. Moura, Isabel; Cunha, Carlos A.; Macieira, Sofia; Dias, João M.; Almeida, Gabriela Machado de; Gonçalves, Luisa L.; Costa, Cristina; et al. 2003. Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774. The gene encoding cytochrome c nitrite reductase(NrfA) from Desulfovibrio desulfuricans ATCC 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-Å resolution. In comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putative substrate inlet and product outlet, and includes a well defined second calcium. http://hdl.handle.net/10362/1658.
  6. Cunha, Carlos A.; Macieira, Sofia; Dias, João M.; Almeida, Gabriela; Gonçalves, Luísa L.; Costa, Cristina; Lampreia, Jorge; et al. 2003. Cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA). The gene encoding cytochrome c nitrite reductase (NrfA) from Desulfovibrio desulfuricans ATCC 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-Å resolution. In comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putative substrate inlet and product outlet, and includes a well defined second calcium. http://www.scopus.com/inward/record.url?scp=0037592981&partnerID=8YFLogxK.
    https://doi.org/10.1074/jbc.M211777200
  7. Almeida, Maria Gabriela; Macieira, Sofia; Gonçalves, Luísa L.; Huber, Robert; Cunha, Carlos A.; Romão, Maria J.; Costa, Cristina; et al. 2003. The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774. The cytochrome c nitrite reductase is isolated from the membranes of the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 as a heterooligomeric complex composed by two subunits (61 kDa and 19 kDa) containing c-type hemes, encoded by the genes nrfA and nrfH,respectively. The extracted complex has in average a 2NrfA:1NrfH composition. The separation of ccNiR subunits from one anothe. http://hdl.handle.net/10362/1657.
    https://doi.org/10.1046/j.1432-1033.2003.03772.x
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/11/22 Biossensores para detectar bactérias| Bactérias para construção de Biossensores Jornadas de Microbiologia e Biologia Molecular
(Caparica, Portugal)
2019/06/03 Inhibition-based biosensor for cyanide detection – a preliminary study 4th International congress of CiiEM - Health, Well Being and Ageing, 2-5 June 2019
Centro de Investigação Interdiciplinar Egas Moniz - CiiEM (Monte da Caparica, Portugal)
2019/06/03 Action of a S. cerevisiae biocide peptide on H.guilliermondii surface 4th International congress of CiiEM - Health, Well Being and Ageing
Centro de Investigação Interdisciplinar Egas Moniz - CiiEM (Monte da Caparica, Portugal)
2018/08/21 Point-of-care testing of nitrites: In the route to market World Congress on Biosensors and Bioelectronics
(Chicago, United States)
2018/06/21 Saccharomycin, a biocide from S. cerevisiae that kill-off other yeasts 3rd Intern. CiiEM Congress “Research and Innovation in Human & Health Sciences
Centro de Investigação Interdisciplinar Egas Moniz - CiiEM (Monte da Caparica, Portugal)
2018/06/20 Voltammetric appraisal of PON1’s activities: a preliminary study 3rd Intern. CiiEM Congress “Research and Innovation in Human & Health Sciences
Centro de Investigação Interdisciplinar Egas Moniz - CiiEM (Monte da Caparica, Portugal)
2018/03/28 Biotechnological Applications of Nitrogen Cycle – Biosensors Nitrogen Cycle Workshop
NOVA - Faculdade de Ciências e Tecnologia (Caparica, Portugal)
2017/11/07 Construction of Electrochemical Biosensors for Nitrite Detection: Needs, Challenges and Perspectives 8th International Workshop on Surface Modification for Chemical and Biochemical Sensing
(Zelechów, Poland)
2017/07/03 Star-shaped gold nanoparticles provide improved interfaces for electrochemical biosensors XXIV Intern. Symposium on Bioelectrochemistry and Bioenergetics
Bioelectrochemistry Society (Lyon, France)
2016/06/06 Point of care testing of nitrites: construction of enzyme biosensors for environmental, food and biomedical applications 8th Meeting of the Analytical Chemistry Division of the Portuguese Chemical Society, Analítica-2016
(Lisbon, Portugal)
2015/10/05 Construction of disposable biosensors for nitrite monitoring in food and beverages 2nd IMEKOFOODS¿“Metrology Promoting Objective and Measurable Food Quality and Safety
(Benevento, Italy)
2015/06/15 Direct electrochemical studies of membrane-bound cytochrome P450 1A2 in water-based sol-gel: with or without P450 reductase? XXIII Intern. Symposium on Bioelectrochemistry and Bioenergetics
The Bioelectrochemistry Society (Malmo, Sweden)
2014/12/09 Sample Preparation in Microbial Proteomics - the case study of Desulfovibrio desulfuricans ATCC 27774 1st Caparica Christmas Conf. on Sample Treatment
(Caparica, Portugal)
2014/04/11 Structural and Functional Features of Cytochrome cd1 Nitrite Reductase 10th Inorganic Chemistry Conference - Portuguese Soc. of Chemistry
Sociedade Portuguesa de Química (Caparica, Portugal)
2014/03/26 “Point of Care Testing of NO metabolites - Construction of biosensing tools to assess nitrite as a vascular endocrine nitric oxide reservoir Sensors in Medicine
(London, United Kingdom)
2013/11/09 Probing the regulation mechanism of cytochrome cd1 nitrite reductase - A combined spectroscopic and electrochemical study 6th Intern. Workshop on Surface Modification for Chemical and Biochemical Sensing
(Lochow, Portugal)
2013/10/23 Dominance of Saccharomyces cerevisiae in wine fermentations: secretion of antimicrobial peptides and microbial interactions 2nd Intern. Conf. on Microbial Diversity: Microbial Interactions in Complex Ecosystems
(Torino)
2013/09/17 Deconvolution of cytochrome c nitrite reductase electrochemistry using MWCNTs with different surface chemistries 3rd Forum on Electrochemistry and Innovation
Univ. Trás-os-Montes (Vila Real, Portugal)
2013/07/08 Electrochemical Means of Detecting Nitrite Ions Department Conferences
Molecular Medicine Branch of the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health (Bethesda, United States)
2012/02/03 Nitrite biosensing with disposable electrode strips – a preliminary study BIODEVICES 2012 – 5th Intern. Conf. on Biomedical Electronics and Devices
(Vilamoura, Portugal)
2011/11/04 Research and Development of Enzyme based Electrochemical Biosensors. The case study of nitrite biosensing 5th Intern. Workshop on Surface Modification for Chemical and Biochemical Sensing
(Lochow, Poland)
2011/09/19 Desenvolvimento de Biossensores Electroquímicos Baseados em Oxidoredutases de Origem Microbiana II Forum Luso-Brasileiro IST-UNL
IST (UTL) (Lisbon, Portugal)
2011/09/08 Construction of a Nitrite Biosensor Based on the Direct Electrochemistry of a Multihemic Nitrite Reductase on Carbon Nanotube Modified Electrodes XII Iberic Meeting of Electrochemistry & XVI Meeting of the Portuguese Electrochem. Society
Sociedade Portuguesa de Electroquímica (Lisbon, Portugal)
2011/09/04 Bacterial diversity and influence of SRB presence on metal behaviour within the oil & gas industry European Corrosion Congress - EUROCORR 2011
(Stockholm, Sweden)
2011/05/10 The Pros and Cons of Nitrite Biosensing through Redox Enzymes REQUIMTE Workshop - Chemical Biology and Bioengineering
REQUIMTE (Caparica, Portugal)
2011/05/09 Joint use of Cytochrome cd1 Nitrite Reductase and its Physiological Electron Donor Cytochrome c552 for Nitrite Biosensing - a New Trend in Mediated Biosensors ” XXI Intern.ational Symposium on Bioelectrochemistry and Bioenergetics
The Bioelectrochemistry Society (Krakow, Poland)
2011/02/11 The Pros and Cons of Nitrite Biosensing through Redox Enzymes 2ª Jornadas de Electroquímica e Inovação
(Faro, Portugal)
2010/09/26 Promising Strategies to Enhance the Direct Electron Transfer of Cytochrome c Nitrite Reductase - Towards the Implementation of an Electrochemical Biosensor 61st Annual Meeting of the International Society of Electrochemistry
(Nice, France)
2010/02/10 Exploring Bacterial Oxidoreductases for Developing New Amperometric Biosensors 38th Conf. Cycle of the Chemistry Dept
(Monte da Caparica, Portugal)
2009/11/16 Comparison Study of Iodine Content in Western Coastal Areas of Portugal 1st Intern. Meeting on Marine Resources
(Peniche, Portugal)
2009/09/02 The impact of high copper concentrations on the Marinobacter aquaeolei proteome 5th Congress of ProCura and 1st Intern. Congress on Analytical Proteomics (ICAP)
Portuguese Proteomics Network (Caparica, Portugal)
2009/09/01 Construction of Catalytic Amperometric Biosensors for Nitrite Detection – A long but promising route 4th Intern. Workshop on "Biosensors for Food Safety and Environmental Monitoring
(Tangier, Morocco)
2008/09/04 Exploring Bacterial Oxido-reductases to Develop Catalytic Amperometric Biosensors for Nitrite Determination XV Meeting of the Portuguese Electrochemical Soc.
(Lisbon, Portugal)
2007/03/30 A Non-Mediated Electrochemical Biosensor for Nitrite Determination 6º Encontro Divisão Química Analítica da Soc. Portuguesa de Química
Sociedade Portuguesa de Química (Lisbon, Portugal)
2006/12/15 Design and construction of electrochemical biosensors for nitrate/nitrite determination. Comparison of different immobilization strategies XX Encontro Nac. Soc. Port. de Química
(Monte da Caparica, Portugal)
2006/06/19 Biossensores: Modernas Ferramentas Analíticas para Monitorização e Controlo da Poluição Ambiental Workshop Biorremediação e Biomonitorização
REQUIMTE & Instituto do Ambiente (Monte Caparica, Portugal)
2006/05/22 Development of an Electrochemical Biosensor for Nitrite Determination in Complex Samples 9th Intern. Symposia on Metal Ions in Biology and Medicine
(Lisbon, Portugal)
2006/03/31 Development of Electrochemical Biosensors for On-line Determination of Nitrite and Nitrate in Complex Samples REQUIMTE – 4th Meeting
(Fátima, Portugal)
2006/02/22 Biossensores: Modernas Ferramentas para Monitorização e Controlo Analítico Ciclo de Conferências "Fascinante Química"
(Vila real, Portugal)
2005/07/31 Multiheme (type c) Membrane Bound Nitrite Reductase from Desulfovibrio desulfuricans ATCC 27774: The Relevance of the Two Calcium Sites in the Catalytic Subunit 12th Intern. Conf. on Biological Inorganic Chemistry - ICBIC12
Society of Biological Inorganic Chemistry (Ann Arbor, United States)
2005/06/23 Construction of Electrochemical Biosensors for Nitrite Determination using Cytochrome c Nitrite Reductase as Biorecognition Molecule - A Comparative Study XVIII International. Symposium on Bioelectrochemistry and Bioenergetics
The Bioelectrochemical Society (Coimbra, Portugal)
2004/04/17 Utilização do Sistema NiR/Nafion/MV na Construção de um Biossensor Voltamétrico Específico para Nitritos XIX Encontro Nacional da Soc. Port. de Química
Sociedade Portuguesa de Química (Coimbra, Portugal)
2003/07/19 Molecular Aspects of Denitrification/Nitrate Dissimilation 11th Intern. Conf. on Biological Inorganic Chemistry
(Cairns, Australia)
1997/11/28 Estudo Comparativo de Iodoperoxidases de Vanádio Extraídas da Alga Ascophyllum nodosum lusitanica XI Encontro Galego-Portugués de Química
(Ferrol, Spain)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2019/10/01 - Current Antimicrobial peptides (AMPs) secreted by Saccharomyces cerevisiae: characterization of chemical structure/function (biological activity), mode of action and potential applications
Supervisor
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/05/01 - Current Development of point-of-care-tests using enzyme (NiR & PON) based electrochemical biosensors
Supervisor
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/03/01 - 2020/07/30 Production and evaluation of recombinant antigens for diagnosis of Strongyloides stercoralis infection and follow up
Supervisor
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/09/01 - 2019/12/19 Desenvolvimento de um biossensor enzimático para a detecção de cianetos em águas e alimentos
Supervisor
 M.Sc. in Chemistry (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2012/07/01 - 2018/06/30 Structural and mechanistic features of cytochrome cd1 nitrite reductase
Supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017/03/15 - 2017/11/25 Avaliação da composição química dos cones de gutta percha utilizados atualmente no tratamento endodôntico
Supervisor
 Mestrado Integrado em Medicina Dentária (Master)
Instituto Superior de Ciências da Saúde Egas Moniz, Portugal
2016/09/05 - 2017/11/13 Identificação de alterações no perfil proteómico de uma estirpe industrial mutante, Saccharomyces cerevisiae PYCC 5334, capaz de consumo simultâneo de glucose e maltose
Co-supervisor
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/10/01 - 2017/05/02 Imobilização de enzimas em matrizes de sol-gel: novos desenvolvimentos em biossensores de nitrito e glucose
Co-supervisor
 MSc. in Biotetechnology (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/10/01 - 2016/12/07 Desenvolvimento de eléctrodos baseados em nanopartículas de WO3 para aplicação como biossensor
Co-supervisor
 MSc in Bioorganic Chemistry (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/09/15 - 2016/11/16 Estudo comparativo dos proteomas extra e intracelular das actinobactérias Streptomyces aculeolatus PTM-29 e PTM-129
Supervisor
 M.Sc. in Biotechnology (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/09/02 - 2016/11/16 Estudo preliminar do metaboloma e proteoma de estirpes da actinobactéria marinha Streptomyces aculeolatus;
Supervisor
 MSc in Biotechnology (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/09/02 - 2016/11/16 Estudo preliminar do metaboloma e proteoma de estirpes da actinobactéria marinha Streptomyces aculeolatus
Co-supervisor
 MSc in Biotechnology (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014/04/01 - 2015/11/03 Purification of antimicrobial peptides secreted by Saccharomyces cerevisiae and proteomic analysis of cell membrane-associated proteins
Supervisor
 MSc in Molecular Biology and Health (Master)
Escola Superior de Saúde Egas Moniz, Portugal
2013/09/10 - 2015/05/27 New Analytical Methodologies to Prevent Cardiovascular Diseases: Development of an Enzyme Biosensor for Homocisteine Detection
Supervisor
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/02/01 - 2014/11/14 Design e fabrico de novos eléctrodos impressos em papel para desenvolvimento de biossensores enzimáticos com aplicações biomédicas
Co-supervisor
 MSc in Biomedical Engineer (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/09/07 - 2014/11/13 Proteómica Microbiana: análise comparativa da bactéria redutora de sulfato Desulfovibrio desulfuricans ATCC 27774 e das actinobactérias Salinispora arenicola e Salinispora pacifica
Supervisor
 MSc. in Biochemistry (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/09/10 - 2013/11/05 Structural and mechanistic features of the denitrifying enzyme cytochrome cd1 nitrite reductase
Supervisor
 MSc. in Biotechnology (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/07/10 - 2013/05/05 Development of oxidoreductase based electrochemical biosensors
Supervisor
 MSC in Biotechnology (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2006/04/01 - 2012/01/19 Construction of a specific sulfites biosensor – comparative study of the catalytic efficiency of bacterial sulfite reductases as biologic recognition elements
Supervisor
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2007/01/02 - 2011/11/25 Employment of nitrite reductase from Desulfovibrio desulfuricans ATCC 27774 for the Development of a Specific Nitrite Biosensor
Supervisor
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010/03/01 - 2011/04/01 Rechargeable Batteries with Aluminum Anode
Supervisor of Fernando Fonseca
 M.Sc. in Renewable Energies - Electrical Conversions and Sustainable Applications
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2008/10/01 - 2009/10/19 Comparison of the Iodine Content in Two Beaches of the West Coast of Continental Portugal
Supervisor of Marta Pimpão
 M.Sc. in Integrative Studies of the Oceans (Master)
Universidade dos Açores, Portugal
2004/10/01 - 2005/09/20 Development of an Electrochemical Biosensor Specific for Nitrites
Supervisor
 Biochemistry (Degree)
Universidade de Lisboa Faculdade de Ciências, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2015/04/01 - 2015/09/30 UCIBIO TRAINING COURSE ON BIOMARKER DISCOVERY USING PROTEOMICS TECHNIQUES: FROM SAMPLE PREPARATION TO PROTEIN VALIDATION; Chem. Dept /FCT/UNl) (2015/08/31 - 2015/09/04)
Other (President of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/04/10 - 2015/06/12 WORKSHOP ON NITRIC OXIDE IN (PATHO)PHYSIOLOGY - FROM MOLECULAR MECHANISMS TO DETECTION; Instituto Superior de Ciências da saúde Egas Moniz (2015/06/12 - 2015/06/12)
Symposium (President of the Organising Committee)
 Egas Moniz Cooperativa de Ensino Superior CRL, Portugal
2014/04/01 - 2014/04/30 WORKSHOP ON BIOSENSORS 7º Encontro Nacional de Estudantes Bioquímica - ENEBIOQ (2014/04/12 - 2014/04/13)
Workshop (President of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010/03/02 - 2010/10/25 5th SHORT COURSE ON BIOELECTROCHEMISTRY (3 days); (2010/10/18 - 2010/10/20)
Other (President of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010/01/02 - 2010/04/30 38th CYCLE OF CONFERENCES at the Chemistry Dept. of NOVA - FCT (Organiser and Chairperson) (2010/01/10 - 2010/04/30)
Conference (Co-organisor)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2010/03/01 - 2010/03/25 2nd SHORT COURSE ON ELECTROCHEMICAL BIOSENSORS; Chemistry Dept., FCT/UNL (2010/10/20 - 2010/10/22)
Other (President of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2009/03/03 - 2009/11/30 1st SHORT COURSE ON ELECTROCHEMICAL BIOSENSORS; Chemistry Dept., FCT/UNL (2009/11/04 - 2009/11/06)
Other (President of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2009/03/02 - 2009/11/30 4th SHORT COURSE ON BIOELECTROCHEMISTRY; (Chem. Dept, FCT/UNL) (2009/11/02 - 2009/11/04)
Other (President of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2008/06/01 - 2008/10/25 ETTAN™ 2-D DIGE TRAINING COURSE (2008/10/08 - 2008/10/10)
Other
 Universidade Nova de Lisboa Faculdade de Ciências Sociais e Humanas, Portugal
2007/03/01 - 2007/03/24 3rd SHORT COURSE ON BIOELECTROCHEMISTRY; (Chem. Dept, FCT/UNL) (2007/10/15 - 2007/10/17)
Other (President of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2006/05/02 - 2006/12/16 SESSION on BIOSENSORS - XXth National Meeting of the Portuguese Chemistry Society (2006/12/14 - 2006/12/16)
Symposium (Co-organisor)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2006/03/02 - 2006/10/21 2nd SHORT COURSE ON BIOELECTROCHEMISTRY; Chem. dept FCT/UNL (2006/10/08 - 2006/10/11)
Other (President of the Organising Committee)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2005/03/01 - 2005/10/30 1st SHORT COURSE ON BIOELECTROCHEMISTRY (2005/10/09 - 2005/10/11)
Other (Co-organisor)
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2019/11/19 Nanostructured surfaces for electrochemical sensors and biosensors and applications
(Thesis) Arguer
 Wanderson da Silva (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2019/07/25 Lab-on-a-chip platforms embedding self-powered electrochemical sensors: a walkthrough approach for bioanalytical applications
(Thesis) Main arguer
 Álvaro Miguel Carneiro Torrinha (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2018/01/18 PURIFICATION AND BIOCHEMICAL/MOLECULAR CHARACTERISATION OF ANTIMICROBIAL PEPTIDES PRODUCED BY SACCHAROMYCES CEREVISIAE AND EVALUATION OF THEIR MODE OF ACTION
(Thesis) Main arguer
 Patrícia Alexandra Batista Branco (PhD)
Universidade de Lisboa Instituto Superior de Agronomia, Portugal
2017/11/13 Identificação de alterações no perfil proteómico de uma estirpe industrial mutante, Saccharomyces cerevisiae PYCC 5334, capaz de consumo simultâneo de glucose e maltose
Supervisor
 Daniela Filipa Tavares Serrano (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/03/10 Studies Towards Unveiling the Association of Gla-Rich Protein With Osteoarthritis
(Thesis) Main arguer
 Sofia Isabel Franco Cavaco (PhD)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal
2016/12/07 Desenvolvimento de eléctrodos baseados em nanopartículas de WO3 para aplicação como biossensor
Supervisor
 Ana Patrícia Mouta Crespo (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/11/16 Estudo comparativo dos proteomas extra e intracelular das actinobactérias Streptomyces aculeolatus PTM-29 e PTM-129
Supervisor
 Lígia Francisca Rodrigues Costa (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/05/16 Effects of ocean warming throughout the life cycle of Sparus aurata: a physiological and proteomic approach
(Thesis) Main arguer
 Diana Sofia Gusmão Coito Madeira (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/04/18 (Bio)funcionalização de superfícies nanoestruturadas para eletrocatálise e desenvolvimento de biossensores eletroquímicos e ótico
(Thesis) Main arguer
 Inês Bela Borralho Almeida (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2016/01/19 The interplay of ascorbic acid, nitric oxide and nitrite in the brain and its role on the regulation of cerebral blood flow: an in vivo electrochemical study
(Thesis) Main arguer
 Nuno Ricardo Esteves Ferreira (PhD)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2015/05/27 Desenvolvimento de novas ferramentas analíticas para a monitorização da actividade da Paraoxonase 1 em amostras de plasma humano
Supervisor
 Francisco Sousa de Oliveira (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014/11/13 Proteómica Microbiana: análise comparativa da bactéria redutora de sulfato Desulfovibrio desulfuricans ATCC 27774 e das actinobactérias Salinispora arenicola e Salinispora pacifica"
Supervisor
 Joana Rita Lourenço Sousa (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/12/16 Disturbed protein arginine methylation in hyperhomocysteinemia
(Thesis) Arguer
 Ruben Miguel Costa Fernandes Esse (PhD)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2013/12/12 Fast screening for diagnostic of heart ischemic episodes
(Thesis) Main arguer
 Felismina Teixeira Coelho Moreira (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/11/05 Características estruturais e mecanísticas da enzima desnitrificante redutase de nitrito citocromo CD1
Supervisor
 Humberto Agostinho Pedroso (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/10/14 Biossensores capacitivos interdigitais para imunodetecção de biomarcadores de Malária
(Thesis) Main arguer
 Tiago Monteiro (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/09/22 Development of Ion Jelly thin films for electrochemical devices
(Thesis) Arguer
 Tânia Isabel da Silva Carvalho (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/11/23 Tyrosinase-Based Phenol Remediation
(Thesis) Main arguer
 Daniel José Rossa Gralheira (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/11/07 Proteomic analysis of a neurodegeneration cell model, treated with plant extracts with potential neuroprotective activity
(Thesis) Main arguer
 Andreia Filipa dos Santos Gomes (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/10/22 Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
(Thesis) Main arguer
 Sofia Matos Flores Évora Garcia (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/01/19 Isolamento e Caracterização de Enzimas Multihémicas de Origem Microbiana e sua Aplicação no Desenvolvimento de Biossensores
Supervisor
 Alexandra Sofia Vasques Gonçalves Serra (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2011/10/14 Development of electrochemical nitrite biosensors using cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
Supervisor
 Célia Marisa Silveira (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Committee member

Activity description
Role 		Institution / Organization
2014/03/01 - 2020/09/01 Coordinator of the M.Sc. in Biomedical Engineering (300 ECTS)
Coordinator
 Instituto Superior de Ciências da Saúde Egas Moniz, Portugal
2020/06/01 - 2020/06/03 Scientific Committee, 5th International Congress of CiiEM (IC CiiEM) Theme: Reducing inequalities in Health and Society
Member
 Centro de Investigação Interdisciplinar Egas Moniz, Portugal
2017/06/03 - 2017/06/13 Scientific Committee, 2nd Intern. CiiEM Congress “Translational Research and Innovation in Human and Health Science
Member
 Centro de Investigação Interdisciplinar Egas Moniz, Portugal
2015/01/02 - 2015/05/13 Scientific Committee, 4th Intern. Conference on Bio-Sensing Technology, Lisbon, Portugal
Member
 Elsevier BV, Netherlands

Institute of Bio-Sensing Technology, UWE, United Kingdom
2009/11/02 - 2011/02/28 Executive Committee, Section Biophysics and Biochemistry (Chemistry Dept)
Member
 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Conference scientific committee

Conference name Conference host
2019/06/02 - 2019/06/05 4th congress of CiiEM on “Health, Well-Being and Ageing in the 21st Century” Caparica, Portugal
2018/06/20 - 2018/06/22 3rd Intern. Congress CiiEM, “Research and Innovation in Human & Health Science" Caparica, Portugal
2015/05/10 - 2015/05/13 4th International Conference on Bio-Sensing Technology Lisbon

Consulting

Activity description Institution / Organization
2018/09/15 - Current Consultant at CLEU Diagnostics LLC (USA), on the project, “Rapid Electrochemical Biosensor for Point-of-Care Diagnosis of Joint Infection”. This project is focused on an innovative technology approach for improving diagnosis of infection after total joint replacement (TJR).

Mentoring / Tutoring

Topic Student name
2012/09/15 - 2013/07/30 Joana R. Sousa
2011/09/15 - 2012/07/30 Sofia Garcia
2011/09/15 - 2012/07/30 Andreia Gomes

Other jury / evaluation

Activity description Institution / Organization
2017/12/01 - 2020/12/31 Investment Expert for the European Commission: evaluator for SMEs (EASME) in the following calls: - H2020-SMEInst-2018-2020-1 - H2020-SMEInst-2018-2020-2 - H2020-EIC-SMEInst-2018-2020-3 Research Executive Agency, Belgium
2016/12/05 - 2017/02/01 Assessment of a research project under the call Bilateral Cooperation between FCT-Portugal and the Ministry of Science and Technology of India. Fundação para a Ciência e a Tecnologia, Portugal
2015/05/15 - 2015/06/20 Invited external referee: evaluation of project proposals for "Young Research Teams" – 2014, Human Resources Programme. Romanian National Authority for Scientific Research and Innovation - Executive Agency for Higher Education, Research, Development and Innovation (UEFISCDI). Romanian National Authority for Scientific Research and Innovation , Romania
Distinctions

Award

2014 Best poster award
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014 Best Poster Award
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Other distinction

2008 Investigador Ciência2007
Fundação para a Ciência e a Tecnologia, Portugal