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Ana Rufino (AR) graduated in Pharmaceutical Sciences from the Faculty of Pharmacy, University of Coimbra, in 2008, and completed her PhD in Pharmacy: Pharmacology and Pharmacotherapy in 2015. She also gained teaching experience between 2014 and 2018 as an invited lecturer in the Nursing program at Escola Superior de Saúde Norte da Cruz Vermelha Portuguesa. Over the past five years, AR has focused on research in biomaterials and tissue engineering. From 2019 to 2023, she was a contracted researcher at LAQV-REQUIMTE, University of Porto, working on advanced scientific studies, in pharmacology, chemistry and . Since February 2023, she has been a Senior Researcher at Metatissue, a biotechnology company specializing in the design, development, and validation of human-based biomaterials for 3D cell culture and tissue engineering, where she leads R&D and D&V processes. Globally, AR co-authored 23 papers in international peer-reviewed journals, most of them with high impact (Q1 and Q2) in the in scientific areas of Drug discovery, Pharmacology, Cell Biology, Molecular biology, Biochemistry and Medicinal chemistry, with 941 citations and 12-h index, being the first author of 8 of them. Ana Rufino also published in other relevant areas as Complementary and alternative medicine, Chemistry and Agricultural and Biological Science. She has delivered multiple oral presentations, including at international conferences, and presented research posters at national and international events. Additionally, abstracts from several of these communications have been published in international peer-reviewed journals.n the last years AR has participated in several high impact financed projects, as a PhD student (PTDC/SAU-OSM/67936/2006) and as executive researcher. In 2021 AR had a FCT financed exploratory project as principal investigator in the area of obesity treatment (EXPL/MED-QUI/0815/2021 49.908,98) and is currently enrolled as a posdoc researcher in several European commission funded projects. AR is internationally recognized as a peer reviewer for multiple journals and a guest editor for Pharmaceutics. AR has supervised students in drug discovery, including MSc, pre-graduate, and PhD students, as well as academic and curricular internships. Her scientific recognition in the Academia also begins to be proven by the invitation to be member of 1 master and 3 graduation juries, all in viva voce oral exam. This contributed to her comprehension of the translational knowledge between academia and industry. Finally, AR acquired a strong background and autonomy in cellular and molecular biology and in molecular pharmacology, both at theoretical and experimental levels.
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Identification

Personal identification

Full name
Ana Teresa Oliveira Rufino

Citation names

  • Rufino, A.T.
  • Rufino A.

Author identifiers

Ciência ID
DC18-6268-7803
ORCID iD
0000-0002-8800-9020
Researcher Id
S-8758-2016

Email addresses

  • anarufino@metatissue.com (Professional)
  • ana.t.rufino@gmail.com (Personal)

Addresses

  • Metatissue PCI · Creative Science Park Aveiro Region Via do Conhecimento, 3830-352 , Ílhavo, Aveiro, Portugal (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences
  • Medical and Health Sciences - Medical Biotechnology
  • Medical and Health Sciences - Basic Medicine - Medicinal Chemistry
  • Medical and Health Sciences - Basic Medicine - Pharmacology and pharmacy
  • Medical and Health Sciences - Other Medical Sciences
  • Medical and Health Sciences - Medical Biotechnology - Biomaterials

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Intermediate (B1) Advanced (C1) Beginner (A1) Advanced (C1)
Education
Degree Classification
2018/10/15 - 2018/11/15
Concluded
 Fundamentals of Project Planning and Management (Outros)
University of Virginia Darden School of Business, United States
 85%
2009 - 2015/01/23
Concluded
 Doutoramento em Farmácia (Doutoramento)
Major in Farmacologia e Farmacoterapia
Universidade de Coimbra, Portugal

Universidade de Coimbra Faculdade de Farmácia, Portugal
"Glucose Sensing and Modulation of Human Chondrocyte Functions by Hyperglycemia: Relevance as Pharmacological Targets for Diabetes -Associated OA" (THESIS/DISSERTATION)
 Distinção e Louvor unanimidade
2012 - 2012
Concluded
 Chondrocyte senescence: implications in osteoarthritis and cartilage tissue (Curso médio)
Universidade de Coimbra, Portugal
 18
2011 - 2011
Concluded
 Biostatistics (Curso médio)
Universidade de Coimbra, Portugal
 16
2010 - 2010
Concluded
 Drug Development (Curso médio)
Universidade de Coimbra, Portugal
 16
2010 - 2010
Concluded
 Cell Damage and Repair (Curso médio)
Universidade de Coimbra, Portugal
 16
2009 - 2009
Concluded
 Obesity & Type II Diabetes (Curso médio)
Universidade de Coimbra, Portugal
 18
2003 - 2008
Concluded
 Mestrado Integrado em Ciências Farmacêuticas (Mestrado)
Universidade de Coimbra, Portugal
"N/A" (THESIS/DISSERTATION)
 16
Affiliation

Science

Category
Host institution 		Employer
2023/02/01 - Current Researcher (Research) Metatissue - Biossolutions Lda, Portugal
2019/02/01 - 2023/01/31 Contracted Researcher (Research) REQUIMTE LAQV Porto, Portugal
2013/01 - 2013/12 Researcher (Research) Universidade de Coimbra, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2014/10 - 2018/10 Adjunct Teacher (Polytechnic Teacher) Escola Superior de Enfermagem da Cruz Vermelha Portuguesa de Oliveira de Azeméis, Portugal

Others

Category
Host institution 		Employer
2009/01 - 2015/01 Estudante (Doutoramento) Universidade de Coimbra, Portugal
2014/02 - 2014/06 Outra Situação (Docente Universitário) Universidade de Coimbra, Portugal
2013/02 - 2013/04 Outra Situação (Docente Universitário) Universidade de Coimbra, Portugal
2011/03 - 2011/06 Outra Situação (Docente Universitário) Universidade de Coimbra, Portugal
2008/09 - 2008/12 Farmacêutico de 1.º Ano (Farmacêutica) Farmácia Pinho, Portugal
Projects

Grant

Designation Funders
2013/01/01 - 2014/01/01 SIMUGROWTH - Desenvolvimento de um modelo computacional para a simulação das propriedades biomecânicas de cartilagem desenvolvida in-vitro em função do estimulo mecânico em bioreactor
PTDC/EME-TME/113039/2009
Research Technician Fellow
Fundação para a Ciência e a Tecnologia
2009/01/01 - 2013/12/31 Actividade metabólica e viabilidade do condrócito em enxertos osteocartilagíneos humanos criopreservados
PTDC/SAU-OSM/67936/2006
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
2009/01 - 2012/12 GLUCOSE SENSING, TRANSPORT AND METABOLISM IN NORMAL AND OSTEOARTHRITIC CHONDROCYTES: MODULATION BY HYPERGLYCAEMIA
Provided by PTCRIS: SFRH/BD/47470/2008
 Fundação para a Ciência e a Tecnologia
2007/09 - 2011/02 Actividade metabólica e viabilidade do condrócito em enxertos osteocartilagíneos humanos criopreservados
Provided by PTCRIS: PTDC/SAU-OSM/67936/2006
 Fundação para a Ciência e a Tecnologia

Contract

Designation Funders
2024/12/01 - Current A bioprinting platform for the rapid, reliable, controlled and quantifiable patterning of cellular aggregates and microtissues into macroscale regenerative grafts with programmable architectures
101191729
Researcher
Metatissue - Biossolutions Lda, Portugal
 European Health and Digital Executive Agency
Ongoing
2023/05/01 - Current Gut Microbiota-Induced Tregs for Inflammatory-Bowel-Disease (IBD) Immunotherapy (MITI2)
101080445
Post-doc
Metatissue - Biossolutions Lda, Portugal
 European Health and Digital Executive Agency
Ongoing
2023/02/01 - Current Urinary bladder bioprinting for fully autologous transplantation
964883
Post-doc
Metatissue - Biossolutions Lda, Portugal
 European Innovation Council and Small and Medium-sized Enterprises Executive Agency
Ongoing
2022/07/01 - 2023/01/31 Screening and pharmacological characterization of flavonoids as lead compounds for obesity treatment
EXPL/MED-QUI/0815/2021
Principal investigator
REQUIMTE LAQV Porto, Portugal
 Fundação para a Ciência e a Tecnologia
2021/09/01 - 2023/01/31 Development of new inhibitors of invasive osteosarcoma based on fisetin derivatives
PTDC/MED-QUI/29243/2017
Researcher
Ongoing
2021/01/01 - 2023/01/31 LA/P/0008/2020
LA/P/0008/2020
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
2020/01/01 - 2023/01/31 UIDB/50006/2020
UIDB/50006/2020
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2020/01/01 - 2023/01/31 UIDP/50006/2020
UIDP/50006/2020
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
2019/03/01 - 2021/08/31 Interdisciplinary in silico, organic synthesis, in vitro and in vivo studies towards new anti-diabetic flavonoid scaffolds
PTDC/MED-QUI/29241/2017
Researcher
2019/02/01 - 2019/02/28 Use of flavonoids in the prophylaxis of intestinal pro-inflammatory effects of silver nanoparticles
PTDC/NAN-MAT/29248/2017
Researcher
Fundação para a Ciência e a Tecnologia
2014/01/01 - 2018 BIORSTIMUL- Desenvolvimento e construção de um novo conceito de bioreactor para a caracterização biomecânica e bioquímica de tecidos de cartilagem desenvolvidos in-vitro
PTDC/EME-PME/103578/2008
Researcher
Fundação para a Ciência e a Tecnologia
2011/01/01 - 2012/12/31 Strategic Project - LA 1 - 2011-2012
PEst-C/SAU/LA0001/2011
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Conference abstract
  1. Rocha, S.; Álvaro Tomé; Rufino, A.T.; Freitas, Marisa; Carvalho, Félix; Silva, Artur; Fernandes, Eduarda. Corresponding author: Fernandes, Eduarda. "Chalcones as potential inhibitors of pancreatic lipase". Paper presented in The 8th International Electronic Conference on Medicinal Chemistry, 2022.
    Published • 10.3390/ ECMC2022-13413
  2. Ribeiro, Madalena; Sousa, Cátia; Rufino, A.T.; Judas, Fernando; Mendes, Alexandrina Ferreira. "Does GPR30 mediate estrogen actions in human chondrocytes? Evaluation of its expression and function". Paper presented in L Annual Meeting of the Portuguese Society of Pharmacology, Coimbra, 2020.
  3. Sousa, Cátia; Ribeiro, Madalena; Rufino, A.T.; Leitão, Alcino Jorge; Mendes, Alexandrina Ferreira. "Validation of a screening assay for GPR30 ligands: an unexpected result". Paper presented in XLV Annual Meeting of the Portuguese Society of Pharmacology, Lisbon, 2015.
  4. Rufino, A.T.; Ribeiro, Madalena; Judas, Fernando; Lopes, Maria C.; Mendes, Alexandrina F.. "Hyperglycemia-like culture conditions induce IL-1beta and TNF-alpha expression and imapir autophagy in human chondrocytes". Paper presented in OARSI - World Congress on Osteoarthritis 2014, Paris, 2014.
    Published
  5. Rufino, Ana Teresa Oliveira; Cavaleiro, Carlos; Judas, Fernando; Salgueiro, Lígia; Lopes, M. Celeste; Mendes, Alexandrina F.. "The essential oil of Eryngium duriae subsp. juresianum inhibits IL-1ß-induced NF-kB and MAPK activation and iNOS expression in human chondrocytes". Paper presented in World Congress on Osteoarthritis 2012, Barcelona, 2012.
    Published
  6. Rufino, A.T.; Judas, Fernando; Salgueiro, Lígia; Lopes, M. Celeste; Cavaleiro, Carlos; Mendes, Alexandrina F.. "Screening of Essential Oils as Potential Sources of Inhibitors of iNOS Expression in Human Chondrocyte". Paper presented in World Congress on Osteoarthritis 2011, San Diego, CA, 2011.
    Published
  7. Rosa, Susana C.; Rufino, A.T.; Judas, Fernando; Lopes, M. Celeste; Mendes, Alexandrina F.. "Role of High Extracellular Glucose concentration in Modulating Anabolic and Catabolic Gene Expression in Normal and Osteoarthritic Chondrocytes". Paper presented in World Congress on Osteoarthritis 2010, Bruxelas, 2010.
    Published
  8. Rufino, A.T.; Rosa, Susana C.; Judas, Fernando; Lopes, M. Celeste; Mendes, Alexandrina F.. "Role of K(ATP) Channels in Modulating GLUT1 content in Normal and AO Human Chondrocytes". Paper presented in World Congress on Osteoarthritis 2010, Bruxelas, 2010.
    Published
Conference paper
  1. José Miguel P. Ferreira de Oliveira; Ana T. Rufino; Carina Proença; Eduarda Fernandes. "In Vitro Cytotoxicity of 3,3',4',7-Tetrahydroxyflavone Derivatives in Human Osteosarcoma". 2022.
    10.3390/ECMC2022-13476
  2. Ana Teresa Rufino; Mariana Lucas; Artur M. S. Silva; Eduarda Fernandes; Daniela Ribeiro. "2-Styrylchromones Inhibit IL-1ß-Induced Inflammatory Mediators’ Production in Human Fibroblast-like Synoviocytes". 2022.
    10.3390/ECMC2022-13183
  3. Sílvia Rocha; Álvaro Tomé; Ana Teresa Rufino; Marisa Freitas; Félix Carvalho; Artur Silva; Eduarda Fernandes. "Chalcones as Potential Inhibitors of Pancreatic Lipase". 2022.
    10.3390/ECMC2022-13413
  4. Bandeiras, C.; Completo, A.; Ramos, A.; Rufino, A.T.; Ribeiro, M.; Ferreira, J.P.; Mendes, A.F.. "Tissue Engineered Cartilage in Unconfined Compression: Biomechanical Analysis". 2015.
    10.1016/j.matpr.2015.04.032
Conference poster
  1. Maria Carolina Lobo; Proença, Carina; Rocha, Silvia; Rufino, A.T.; Carvalho, Félix; Silva, Artur; Fernandes, Eduarda. Corresponding author: Fernandes, Eduarda. "Flavonoids as potential anti-obesogenic agents through modulation of pancreatic lipase and protein tyrosine phosphatase 1B: a structure-activity relationship". Paper presented in 57th International Conference on Medicinal Chemistry, 2023.
  2. Rufino, A.T.; Lucas, Mariana; Silva, Artur M.S.; Fernandes, Eduarda; Ribeiro, Daniela. "2-Styrylchromones inhibit IL-1ß-induced inflammatory mediators' production in human fibroblast-like synoviocytes". Paper presented in 8th International Electronic Conference on Medicinal Chemistry, 2022.
  3. "Evaluation of cytotoxic potential of polyhydroxylated phenylchromones in human osteosarcoma in vitro models". Paper presented in CBTOX 2021 - XXII BRAZILIAN CONGRESS OF TOXICOLOGY, 2022.
  4. Maria Carolina Lobo; Rocha, S.; Carvalho, Félix; Silva, Artur; Fernandes, Eduarda; Rufino A.. "In vitro activity of polyphenols against pancreatic lipase and a-amylase inhibition". Paper presented in IJUP - Young Researchers Meeting, 2022.
  5. Proença, Carina; Freitas, Marisa; Rufino A.; de Oliveira, José Miguel P. Ferreira; Silva, Artur M. S.; Fernandes, Pedro Alexandrino; Fernandes, Eduarda. "Dual inhibition of carbohydrate-hydrolysing enzymes a-amylase and a-glucosidase by flavonoids". Paper presented in 14.º Encontro Nacional de Química Orgânica (14ENQO) e 7.º Encontro Nacional de Química Terapêutica (7ENQT), 2022.
  6. Rufino, A.T.. "Evaluation of cytotoxic and pro-inflammatory effects of silver nanoparticles in gastrointestinal tract in vitro models: Potential protective effect of flavonoids". Paper presented in 14th National Organic Chemistry Meeting & 7th National Medicinal Chemistry Meeting, 2022.
  7. Rufino, A.T.. "The possible role of flavonoids on postprandial hyperglycaemia through inhibition of carbohydrate-hydrolysing enzymes". Paper presented in 14th National Organic Chemistry Meeting & 7th National Medicinal Chemistry Meeting, 2022.
  8. Rufino, A.T.. "Polyphenols’ anti-obesity potential evaluation in in vitro microanalysis systems based on pancreatic lipase and a-amylase inhibition". Paper presented in 14th National Organic Chemistry Meeting & 7th National Medicinal Chemistry Meeting, 2022.
  9. Rufino, A.T.. "Evaluation of antiproliferative and apoptotic effects of flavonoids in osteosarcoma in vitro models". Paper presented in 14th National Organic Chemistry Meeting & 7th National Medicinal Chemistry Meeting, 2022.
  10. Rufino, A.T.. "Silver nanoparticles exert harmful effects in human monocytes". Paper presented in 14th National Organic Chemistry Meeting & 7th National Medicinal Chemistry Meeting, 2022.
  11. Maria Carolina Lobo; Rocha, S.; Rufino A.; Carvalho, Félix; Silva, Artur; Fernandes, Eduarda. "Inhibition of Pancreatic Lipase and a-Amylase by Polyphenols in In Vitro Microanalysis Systems". Paper presented in 14th Edition of the Nacional Organic Chemistry Meeting and 7th Edition of the Nacional Therapeutic Chemistry Meeting, 2022.
  12. Rufino, A.T.. "Size and coating dependent pro-inflammatory effects of AgNP in human monocytes and macrophages". Paper presented in SOT 61st Annual Meeting and Expo, 2022.
  13. Rufino, A.T.. "Effect of phenolic compounds on the apoptotic process in human osteosarcoma cells". Paper presented in 7th International Electronic Conference on Medicinal Chemistry, 2021.
  14. Rufino, A.T.. "Evaluation of the inhibitory activity of flavonoids on sucrase-isomaltase (a-glucosidase) in a Caco-2/TC7 cellular model". Paper presented in 56th International Conference on medicinal Chemistry, 2021.
  15. Rufino, A.T.. "Evaluation of the anti-obesity potential of polyphenols through Pancreatic Lipase Inhibition". Paper presented in Encontro Nacional de estudantes de Bioquímica 2021, 2021.
  16. Rufino, A.T.. "Flavonoids Modulate the Cytotoxic Effect of Silver Nanoparticles". Paper presented in EuroNanoForum 2021, 2021.
  17. Rufino, A.T.. "The protective effect of flavonoids against silver nanoparticles-induced intestinal cell death". Paper presented in IberPhenol International Conference, 2020.
  18. Rufino, A.T.. "Testing the effects of silver nanoparticles in in vitro models of intestinal toxicity and inflammation". Paper presented in Bionanotox 2019, 2019.
  19. Rufino, A.T.. "Hyperglycemia-like culture conditions induce IL-1B and TNF-a expression and impair autophagy in human chondrocytes". Paper presented in World Congress on Osteoarthritis 2014, 2014.
    http://dx.doi.org/10.1016/j.joca.2014.02.308
  20. Rufino, A.T.; Rufino, Ana Teresa Oliveira; Ribeiro, Madalena; Judas, Fernando; Lopes, M. Celeste; Mendes, Alexandrina F.. "Culture of human chondrocytes in high glucose induces inflammatory markers and impairs autophagy". Paper presented in 34th European Workshop for Rheumatology Research, 2014.
    10.1136/annrheumdis-2013-205124.143
  21. Rufino, A.T.. "The essential oil of Eryngium Duriae subsp juresianum inhibits IL-1ß induced NF-KappaB and MAPK activation and iNOS expression in human chondrocytes". Paper presented in 43rd International Symposium on Essential Oils, 2012.
    http://dx.doi.org/10.1016/j.joca.2012.02.502
  22. Rufino, A.T.. "The essential oil of Eryngium Duriae subsp juresianum inhibits IL-1ß induced NF-kappaB and MAPK activation in human chondrocytes". Paper presented in world Congress on Osteoarthritis 2012, 2012.
  23. Rufino, A.T.. "Inhibition of IL-1ß-induced inflammatory and catabolic pathways by the essential oil of Eryngium duriae subsp. juresianum in human chondrocytes". Paper presented in European Congress of Pharmacology - EPHAR 2012, 2012.
  24. Ferreira Mendes, A.; Rosa, Susana; Rufino, A.T.; Judas, F.M; Salgueiro, L.; Lopes, M.C.; Cavaleiro, Carlos. "DUAL INHIBITION OF IL-1-INDUCED NF-KB ACTIVATION AND INOS ACTIVITY IN HUMAN CHONDROCYTES BY ALPHA-PINENE.". Paper presented in 10th World Congress on inflammation. Paris, France, 2011.
  25. Rufino, A.T.; Rosa, S.C.; Judas, F.; Tenreiro, C.; Lopes, M.C.; Mendes, A.F.. "Expression and function of the insulin receptor in normal and osteoarthritic human chondrocytes: modulation of anabolic gene expression, glucose transport and GLUT-1 content by insulin". 2011.
    http://dx.doi.org/10.1016/j.joca.2011.02.004
  26. Rufino, A.T.. "Screening of Essential Oils as Potential Sources of Inhibitors of iNOS Expression in Human Chondrocytes". Paper presented in World Congress on Osteoarthritis 2011, 2011.
  27. Rufino, A.T.. "ROLE OF K(ATP) CHANNELS IN MODULATING GLUT-1 CONTENT IN NORMAL AND OSTEOARTHRITIC HUMAN CHONDROCYTES". 2010.
    http://dx.doi.org/10.1016/S1063-4584(10)60241-5
  28. Moitra, J; Chiang, ET; Brown, ME; Singleton, PA; Garcia, JG. "Role of High Extracellular Glucose concentration in Modulating Anabolic and Catabolic Gene Expression in Normal and Osteoarthritic Chondrocytes". 2010.
    10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a4179
  29. Rufino, A.T.. "Differential regulation of GLUT-1 content by the K(ATP) channel blocker, Glibenclamide, in normal and Osteoarthritic chondrocytes". Paper presented in Congresso Nacional de Bioquímica, 2010.
    http://dx.doi.org/10.1016/S1063-4584(10)60222-1
  30. Rosa, Susana; Rufino, A.T.; Judas, F.M; Lopes, M.C.; Ferreira Mendes, A.. "Role of high extracellular glucose concentrations in modulating anabolic and catabolic gene expression in normal and osteoarthritic human chondrocytes.". Paper presented in 15th World Congress on Osteoarthritis. Brussels, Belgium., 2009.
  31. Rufino, A.T.; Rosa, Susana; Judas, F.M.; Mobasheri, A.; Lopes, M.C.; Ferreira Mendes, A.. "Role of K(ATP) channels in modulating GLUT-1 content in normal and osteoarthritic chondrocytes.". Paper presented in 15th World Congress on Osteoarthritis. Brussels, Belgium., 2009.
Journal article
  1. Proença, Carina; Rufino, Ana Teresa; Santos, Isabela; Albuquerque, Hélio M. T.; Silva, Artur M. S.; Fernandes, Eduarda; Ferreira de Oliveira, José Miguel P.. "Gossypetin Is a Novel Modulator of Inflammatory Cytokine Production and a Suppressor of Osteosarcoma Cell Growth". Antioxidants 12 9 (2023): 1744. http://dx.doi.org/10.3390/antiox12091744.
    10.3390/antiox12091744
  2. Rocha, Sílvia; Rufino, Ana T.; Freitas, Marisa; Silva, Artur M. S.; Carvalho, Félix; Fernandes, Eduarda. "Methodologies for Assessing Pancreatic Lipase Catalytic Activity: A Review". Critical Reviews in Analytical Chemistry (2023): 1-28. http://dx.doi.org/10.1080/10408347.2023.2221731.
    10.1080/10408347.2023.2221731
  3. Proença, Carina; Freitas, Marisa; Ribeiro, Daniela; Rufino, Ana T.; Fernandes, Eduarda; Ferreira de Oliveira, José Miguel P.. "The role of flavonoids in the regulation of epithelial-mesenchymal transition in cancer: A review on targeting signaling pathways and metastasis". Medicinal Research Reviews 43 6 (2023): 1878-1945. http://dx.doi.org/10.1002/med.21966.
    10.1002/med.21966
  4. Ana Teresa Rufino; Mariana Lucas; Artur M. S. Silva; Daniela Ribeiro; Eduarda Fernandes. "2-Styrylchromones Prevent IL-1ß-Induced Pro-Inflammatory Activation of Fibroblast-like Synoviocytes while Increasing COX-2 Expression". Pharmaceutics (2023): https://doi.org/10.3390/pharmaceutics15030780.
    10.3390/pharmaceutics15030780
  5. Sousa, Adelaide; Rufino, Ana T.; Fernandes, Rui; Malheiro, Ana; Carvalho, Félix; Fernandes, Eduarda; Freitas, Marisa. "Silver nanoparticles exert toxic effects in human monocytes and macrophages associated with the disruption of ¿¿m and release of pro-inflammatory cytokines". Archives of Toxicology 97 2 (2022): 405-420. http://dx.doi.org/10.1007/s00204-022-03415-x.
    10.1007/s00204-022-03415-x
  6. Rufino A.; Freitas, Marisa; Proença, Carina; de Oliveira, José Miguel P. Ferreira; Fernandes, Eduarda; Daniela RIbeiro; Rufino, Ana T.; et al. Corresponding author: Rufino A.. "Rheumatoid arthritis molecular targets and their importance to flavonoid based therapy". Medicinal Research Reviews 44 2 (2022): 497-538. http://dx.doi.org/10.1002/med.21990.
    Under revision • 10.1002/med.21990
  7. Carina Proença; Ana T. Rufino; José Miguel P. Ferreira de Oliveira; Marisa Freitas; Pedro A. Fernandes; Artur M. S. Silva; Eduarda Fernandes. "Inhibitory activity of flavonoids against human sucrase-isomaltase (a-glucosidase) activity in a Caco-2/TC7 cellular model". Food & Function 13 3 (2022): 1108-1118. https://doi.org/10.1039/D1FO02995A.
    10.1039/D1FO02995A
  8. Rufino, Ana T.; Ramalho, Ana; Sousa, Adelaide; de Oliveira, José Miguel P. Ferreira; Freitas, Paulo; Gómez, Manuel A. Gonzalez; Piñeiro-Redondo, Yolanda; et al. "Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles". Molecules 26 21 (2021): 6610. http://dx.doi.org/10.3390/molecules26216610.
    Open access • Published • 10.3390/molecules26216610
  9. Rufino, Ana T.; Costa, Vera M.; Carvalho, Félix; Fernandes, Eduarda. Corresponding author: Fernandes, Eduarda. "Flavonoids as antiobesity agents: A review". Medicinal Research Reviews (2021): http://dx.doi.org/10.1002/med.21740.
    Published • 10.1002/med.21740
  10. Ribeiro, Madalena; Sousa, Cátia; Rufino, Ana T.; Judas, Fernando; Mendes, Alexandrina F.. "Expression and function of the nonclassical estrogen receptor, GPR30, in human cartilage and chondrocytes". Journal of Cellular Physiology (2020): http://dx.doi.org/10.1002/jcp.29691.
    10.1002/jcp.29691
  11. Ribeiro, Daniela; Sousa, Adelaide; Nicola, Pedro; Ferreira de Oliveira, José Miguel P.; Rufino, Ana T.; Silva, Marta; Freitas, Marisa; Carvalho, Félix; Fernandes, Eduarda. "ß-Carotene and its physiological metabolites: Effects on oxidative status regulation and genotoxicity in in vitro models". Food and Chemical Toxicology (2020): 111392. http://dx.doi.org/10.1016/j.fct.2020.111392.
    10.1016/j.fct.2020.111392
  12. Rufino, A.T.. "Hyperglycemia and Hyperinsulinemia-Like Conditions Independently Induce Inflammatory Responses in Human Chondrocytes". Journal of Functional Morphology and Kinesiology (2017): http://www.mdpi.com/2411-5142/2/2/15.
    10.3390/jfmk2020015
  13. Sousa, C.; Ribeiro, M.; Rufino, A.T.; Leitão, A.J.; Mendes, A.F.; Sousa C; Ribeiro M; et al. "Assessment of cell line competence for studies of pharmacological GPR30 modulation". Journal of Receptors and Signal Transduction 37 2 (2017): 181-188. http://www.scopus.com/inward/record.url?eid=2-s2.0-84978069234&partnerID=MN8TOARS.
    10.1080/10799893.2016.1203943
  14. Rufino, Ana Teresa; Ribeiro, Madalena; Sousa, Cátia; Judas, Fernando; Salgueiro, Lígia; Cavaleiro, Carlos; Mendes, Alexandrina Ferreira. "Evaluation of the anti-inflammatory, anti-catabolic and pro-anabolic effects of E-caryophyllene, myrcene and limonene in a cell model of osteoarthritis". European Journal of Pharmacology 750 5 (2015): 141-150.
    Published • 10.1016/j.ejphar.2015.01.018
  15. Rufino, A.T.; Ferreira, I.; Judas, F.; Salgueiro, L.; Lopes, M.C.; Cavaleiro, C.; Mendes, A.F.. "Differential effects of the essential oils of Lavandula luisieri and Eryngium duriaei subsp. juresianum in cell models of two chronic inflammatory diseases". Pharmaceutical Biology 53 8 (2015): 1220-1230. http://www.scopus.com/inward/record.url?eid=2-s2.0-84930789356&partnerID=MN8TOARS.
    10.3109/13880209.2014.970701
  16. Mendes AF; Rosa SC; Rufino AT; Ribeiro M; Judas F. "Diabetes-induced osteoarthritis: role of hyperglycemia in joint destruction.". (2015): http://europepmc.org/abstract/PMC/PMC4674852.
  17. Rufino, Ana T.; Ribeiro, Madalena; Judas, Fernando; Salgueiro, Lígia; Lopes, Maria C.; Cavaleiro, Carlos; Mendes, Alexandrina F.. "Anti-inflammatory and Chondroprotective Activity of (+)-a-Pinene: Structural and Enantiomeric Selectivity". Journal of Natural Products 77 2 (2014): 264-269.
    Published • 10.1021/np400828x
  18. Serra, Diana; Rufino, Ana T.; Mendes, Alexandrina F.; Almeida, Leonor M.; Dinis, Teresa C. P.; Singh, Shree Ram. "Resveratrol Modulates Cytokine-Induced JAK/STAT Activation More Efficiently than 5-Aminosalicylic Acid: An In Vitro Approach". PLoS ONE 9 10 (2014): e109048-e109048.
    Published • 10.1371/journal.pone.0109048
  19. Rufino, Ana T.; Rosa, Susana C.; Judas, Fernando; Mobasheri, Ali; Lopes, M. Celeste; Mendes, Alexandrina F.; Rufino, A.T.; et al. "Expression and function of K(ATP) channels in normal and osteoarthritic human chondrocytes: possible role in glucose sensing". Journal of Cellular Biochemistry 114 8 (2013): 1879-1889. https://onlinelibrary.wiley.com/doi/full/10.1002/jcb.24532.
    10.1002/jcb.24532
  20. Mobasheri, Ali; Lewis, Rebecca; Ferreira-Mendes, Alexandrina; Rufino, Ana; Dart, Caroline; Barrett-Jolley, Richard. "Potassium channels in articular chondrocytes". Channels 6 6 (2012): 416-425.
    Published • 10.4161/chan.22340
  21. Rosa, Susana C.; Rufino, Ana T.; Judas, Fernando M.; Tenreiro, Carlos M.; Lopes, Maria C.; Mendes, Alexandrina F.. "Role of glucose as a modulator of anabolic and catabolic gene expression in normal and osteoarthritic human chondrocytes". Journal of Cellular Biochemistry 112 10 (2011): 2813-2824.
    Published • 10.1002/jcb.23196
  22. Rosa, S.C.; Rufino, A.T.; Judas, F.; Tenreiro, C.; Lopes, M.C.; Mendes, A.F.. "Expression and function of the insulin receptor in normal and osteoarthritic human chondrocytes: Modulation of anabolic gene expression, glucose transport and GLUT-1 content by insulin". Osteoarthritis and Cartilage 19 6 (2011): 719-727. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955972378&partnerID=MN8TOARS.
    10.1016/j.joca.2011.02.004
  23. Neves, Â.; Rosa, S.; Gonçalves, J.; Rufino, A.; Judas, F.; Salgueiro, L.; Lopes, M.C.; Cavaleiro, C.; Mendes, A.F.. "Screening of five essential oils for identification of potential inhibitors of IL-1-induced Nf-kB activation and NO production in human chondrocytes: Characterization of the inhibitory activity of a-pinene". Planta Medica 76 3 (2010): 303-308. http://www.scopus.com/inward/record.url?eid=2-s2.0-77049099601&partnerID=MN8TOARS.
    10.1055/s-0029-1186085
Newsletter article
  1. Sousa, Adelaide; Rufino, Ana T.; Ana Ramalho; Carvalho, Félix; Fernandes, Eduarda; Freitas, Marisa. "Intestinal inflammation mediated by silver nanoparticles is prevented by dietary flavonoids", Atlas of Science, 2022, https://atlasofscience.org/intestinal-inflammation-mediated-by-silver-nanoparticles-is-prevented-by-dietary-flavonoids/.
Thesis / Dissertation
  1. Mendes, Alexandrina Ferreira; Cavaleiro, Carlos. "Glucose Sensing and Modulation of Human Chondrocyte Functions by Hyperglycemia: Relevance as Pharmacological Targets for Diabetes – Associated OA". PhD, 2015. http://hdl.handle.net/10316/26252.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/06/25 Protective role of flavonoids against intestinal pro-inflammatory effects of silver nanoparticles Webinar "The Interaction of Biomedical Nanoparticles with the Immune System"
MDPI Webinars - Cells 2021 webinars
2020/11/09 À descoberta das atividades biológicas de flavonoides Special Topics in Food and Nutrition - 30hours course
University of Campinas - Faculty of Food Nutrition (Campinas, Brazil)
2016/09 Nursing intervention in the promotion of the transition to parenthood 3rd World Congress of Health Research
(Viseu, Portugal)
2015/02 Validation of a screening assay for GPR30 ligands: an unexpected result XLV Reunião Anual da Sociedade Portuguesa de Farmacologia
(Coimbra, Portugal)
2015 Diabetes-induced osteoarthritis: role of hyperglycemia in joint destruction Proceedings in Musculoskeletal Health in the 21st Century Workshop
(Guildford, United Kingdom)
2014/02 Hyperglycemia induces inflammatory mediators in human chondrocytes: inhibition by the essential oil of Lavandula luisieri XLIV Reunião Anual da Sociedade Portuguesa de Farmacologia
(Coimbra, Portugal)
2013/12 Diabetes-Associated Osteoarthritis: Unraveling pathological mechanisms and pharmacological targets CNC seminars
(Coimbra, Portugal)
2013/12 Anti-inflammatory and chondroprotective activity of natural compounds myrcene and limonene IV Conferência CEF
Centro de Estudos Farmacêuticos (Coimbra, Portugal)
2013/02 Inhibition of inflammatory and catabolic responses in human chondrocytes by (+) a-pinene XLIII Reunião da Sociedade Portuguesa de Farmacologia
(Porto, Portugal)
2012/07 Pharmacological screening of essential oils for identification of NF-kappaB and iNOS inhibitors in a human intestinal epithelial cell line 6th European Congress of Pharmacology - EPHAR
(Granada, Spain)
2012/02 Inhibition of IL-1ß Induced Pro-Inflammatory and Catabolic Signaling Pathways by The Essential Oil of Eryngium Duriaei Subsp. Juresianum In Human Chondrocytes XLII Reunião da Sociedade Portuguesa de Farmacologia
(Lisboa, Portugal)
2011/02 Modulation of ATP-sensitive Potassium Channels Mediates the Regulation of GLUT-1 Content in Articular Chondrocytes: Differential role in normal and Osteoarthritic Chondrocytes XLI Reunião Anual da Sociedade Portuguesa de Farmacologia
(Coimbra, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2024/09 - Current Advancing in vitro vascularization with human protein-based materials in 3D cell culture systems
Supervisor
 Bioquímica (Master)
Metatissue - Biossolutions Lda, Portugal

Universidade de Aveiro, Portugal
2021/09 - Current Screening de polifenois e determinação de estruturas- modelo para o desenvolvimento de novas moléculas eficazes no tratamento da obesidade.
Supervisor
 Mestrado em Controlo de Qualidade (Master)
Universidade do Porto Faculdade de Farmácia, Portugal
2023/09 - 2024/09 Comparative study of the influence of 3D biological matrices on cell culture: hPLMA vs. Matrigel
Supervisor
 Bioquímica (Master)
Metatissue - Biossolutions Lda, Portugal

Universidade de Aveiro, Portugal
2023/03/27 - 2023/07 OTIMIZAÇÃO DO MÉTODO DE RECUPERAÇÃO DE CÉLULAS ENCAPSULADAS EM HIDROGÉIS DE PLMA
Supervisor
Instituto Politécnico da Guarda Escola Superior de Saúde, Portugal
2021/09 - 2023/01/31 Avaliação dos efeitos de compostos flavonoides na viabilidade celular e inflamação em modelo celular tridimensional de osteossarcoma.
Co-supervisor
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/09 - 2022/09 Polyphenols as potential anti-obesity molecules
Supervisor
 Licenciatura em Bioquímica (Degree)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/02/01 - 2021/07/08 Polyphenols as potential anti-obesity molecules
Supervisor of Maria Carolina Ribeiro Marujo e Rocha Lobo
 Bioquímica (Degree)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2020/02/01 - 2020/07/28 Role of non-nutritional sweeteners on the development and progression of chronic inflammatory diseases
Supervisor of André Miguel Dias
 Licenciatura em Bioquímica (Degree)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2013 - 2013 Papel dos estrogénios na modulação da capacidade proliferativa e de diferenciação condrogénica de células estaminais do tecido adiposo humano
Co-supervisor
2011 - 2012 Identificação e caracterização farmacológica de novos compostos de origem natural para o tratamento de doenças inflamatórias crónicas
Co-supervisor
2010 - 2011 Pharmacological screening of essential oils for identification of lead compounds to target Inflammatory Bowel Disease
Co-supervisor
2010 - 2011 Identificação e caracterização farmacológica de novos compostos de origem natural para o tratamento de doenças inflamatórias crónicas
Co-supervisor
2009 - 2010 Role of ATP-sensitive potassium channels in the regulation of NO production by the neuronal nitric oxide synthase (nNOS) in human chondrocytes
Co-supervisor

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2022/02/18 Atividade inibitória da enzima a-glucosidase por estirilcromonas e estirilpirazóis
(Thesis) Main arguer
 Joana Maria carvalho Correia (Other)
Universidade do Porto Faculdade de Farmácia, Portugal
2021/07/08 Efeito protetor de flavonoides em neutrófilos ativados por nanopartículas de prata
(Thesis) Main arguer
 Jorge Miguel Almeida Silva (Other)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/07/07 Screening of structurally-related flavonoids on cell viability and evaluation of effects on reactive species formation in an osteosarcoma in vitro model
(Thesis) Main arguer
 Beatriz Gandra Moreira (Other)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
2016/09 New therapeutic strategies for osteoarthritis: Injective cell therapy
(Thesis) Main arguer
 Sylvie Gonçalves (Master)
Universidade de Coimbra Faculdade de Farmácia, Portugal

Association member

Society Organization name Role
2008/08 - Current Ordem dos Farmacêuticos

Committee member

Activity description
Role 		Institution / Organization
2017/06 - 2017/07 Member of the scientific committee of the 10º Encontro Científico &Inovação, Empreendedorismo e Liderança em Saúde
Member
 Escola Superior de Saúde Norte da Cruz Vermelha Portuguesa, Portugal
2017/01 - 2017/04 Member of the organization committee of the III Conferência Internacional de Investigação em Saúde
Member
 Escola Superior de Saúde Norte da Cruz Vermelha Portuguesa, Portugal
2017/01 - 2017/01 Member of the scientific committee of the III Conferência Internacional de Investigação em Saúde
Member
 Escola Superior de Saúde Norte da Cruz Vermelha Portuguesa, Portugal

Conference scientific committee

Conference name Conference host
2022/05/04 - 2022/05/06 IJUP 2022 - Investigação Jovem da Universidade do Porto Porto

Consulting

Activity description Institution / Organization
2015 - 2017 Project: Research and development of a drug that modifies the evolution of osteoarthritic disease Partners: Bluepharma, Pharmaceutical Industry, SA, Centre for Neuroscience and Cell Biology and Faculty of Pharmacy, University of Coimbra and University of Minho Current Status: 1st phase (proof of concept in vivo) completed; ongoing: i) preparation of the patent application; ii) programming and definition of the methodology for validating the results of the first phase.

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2021/10 - 2022/03 Química Geral e Inorgânica Mestrado Integrado em Ciências Farmaceuticas (Mestrado integrado) Universidade do Porto Faculdade de Farmácia, Portugal
2014/09 - 2018/10 Farmacologia Licenciatura em Enfermagem (Licenciatura) Escola Superior de Saúde Norte da Cruz Vermelha Portuguesa, Portugal
2014/09 - 2018/10 Bioquimica e Microbiologia Licenciatura em Enfermagem (Licenciatura) Escola Superior de Saúde Norte da Cruz Vermelha Portuguesa, Portugal
2014/09 - 2018/10 Nutrição Licenciatura em Enfermagem (Licenciatura) Escola Superior de Saúde Norte da Cruz Vermelha Portuguesa, Portugal
2014/09 - 2018/10 Investigação I Licenciatura em Enfermagem (Licenciatura) Escola Superior de Saúde Norte da Cruz Vermelha Portuguesa, Portugal
2014/02 - 2014/06 Anatomofisiologia Humana Mestrado Integrado em Ciências Farmacêuticas (Mestrado integrado) Universidade de Coimbra, Portugal
2013/02 - 2013/04 Anatomofisiologia Humana (Outros) Universidade de Coimbra, Portugal
2011/03 - 2011/06 Anatomofisiologia Humana (Outros) Universidade de Coimbra, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2021/05/05 - Current Brazilian Journal of Pharmaceutical Sciences Scielo
2021/04/30 - Current Antioxidants MDPI
2021/04/29 - Current Molecules MDPI
2021/03/03 - Current Journal of Clinical Medicine MDPI
2021 - Current Nutrients MDPI
2019/04/11 - Current Annals of Translational Medicine AME Publishing Company
2019/01/30 - Current Medical Science Monitor International Scientific Information
2017/08/31 - Current Scientific Reports (2045-2322) Springer Nature
2017/08/15 - Current Letters in Drug Design & Discovery (1570-1808) Bentham Science
2017/07/31 - Current Cancer Biomarkers (1875-8592) IOS Press
2017/07/20 - Current Biomedicine & Pharmacotherapy (0753-3322) Elsevier
2017/03/24 - Current Experimental Cell Research (1090-2422) Elsevier
2017/03/07 - Current Pharmaceutical Biology (1744-5116) Informa UK (Taylor & Francis)
2017/02/02 - Current Journal of Pharmacy and Pharmacology (2042-7158) Wiley (Blackwell Publishing)
2016/08/17 - Current PLoS ONE (1932-6203) Public Library of Science

Mentoring / Tutoring

Topic Student name
2021/10/01 - Current Targeting Pancreatic lipase for obesity treatment: The role of Polyphenols Sílvia Daniela Gonçalves da Rocha