Diana Duarte Lobo (D91F-7184-F767)

Curriculum Vitae

Diana Duarte Lobo

Diana Duarte Lobo completed the Master degree in Molecular Genetics and Biomedicine in 2017 by Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia and the Bachelor in Biochemistry in 2015 by Universidade de Coimbra Faculdade de Ciencias e Tecnologia. Her Master thesis has resulted in an original article published in Acta Neurpathological Communications. She participates and/or participated as Research Fellow in several projects, some involving pharmaceutical companies such as, Wave Life Sciences, uniQure and Blade Therapeutics. She has received a scholarship to attend to SPN Meeting 2019. Her work has produced 6 oral and poster communications as presenting author in national conferences. Diana published 2 articles in peer-reviewed journals (eg. Brain and Molecular Therapy) and 2 articles as first author, one of them recently accepted in Nucleic Acid Therapeuthics journal. Diana works/worked in the areas of Neurosciences and Gene Therapy targeting the brain, mainly in the context of Machado-Joseph disease. Currently, Diana is a PhD student at Center for Neuroscience and Cell Biology of University of Coimbra. Her thesis is entitled “Overcoming the major roadblocks for successful AAV-based gene therapy in CNS”.

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Identification

Personal identification

Full name: Diana Duarte Lobo

Citation names

Lobo, Diana Duarte

Author identifiers

Ciência ID: D91F-7184-F767
ORCID iD: 0000-0001-5785-4283

Knowledge fields

Medical and Health Sciences - Basic Medicine - Neurosciences
Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Upper intermediate (B2)	Advanced (C1)	Advanced (C1)	Advanced (C1)

Education

	Degree	Classification
2021/01/01 - 2025/01/01 Ongoing	Biologia Experimental e Biomedicina (Doutoramento) Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal "Overcoming the major roadblocks for successful AAV-based gene therapy in CNS" (THESIS/DISSERTATION)
2019/11/04 - 2019/11/15 Concluded	III Modular Structure Training in Laboratory Animal Science (Outros) Coimbra Institute for Clinical and Biomedical Research, Portugal Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	17,1
2015/09 - 2017/11 Concluded	Genética Molecular e Biomedicina (Mestrado) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal "Cerebrovascular and Blood-brain Barrier Impairments in Machado-Joseph Disease" (THESIS/DISSERTATION)	18
2012/09 - 2015/07 Concluded	Bioquímica (Licenciatura) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal	15

Affiliation

Others

	Category Host institution	Employer
2021/01/01 - Current	PhD student at Gene and Stem Cell Therapies for the brain, CNC	Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

2022/04 - 2022/06	Research Trainee at CNS Gene Therapy group, Lund University	Lunds Universitet, Sweden

2018/05/11 - 2020/12/31	Research Fellow at Gene and Stem Cell Therapies for the brain, CNC	Universidade de Coimbra, Portugal
	Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2015/02 - 2015/07	Trainee	Centro de Sangue e daTransplantação de Coimbra – Áreas Funcionais do Sangue e da Transplantação, Portugal
	Centro de Sangue e daTransplantação de Coimbra – Áreas Funcionais do Sangue e da Transplantação, Portugal

Projects

Grant

	Designation	Funders
2021/01/01 - Current	Accelerating Research & Development for Advanced Therapies (ARDAT) 945473 PhD Student Fellow Innovative Health Initiative, Belgium	European Union European Federation of Pharmaceutical Industries and Associations Ongoing
2018/05/11 - Current	BrainHealth 2020: Deteção Precoce, Neuro-modulação e Terapias Avançadas para Neuropatologias SAICT 000008 BrainHealth 2020 Research Fellow CNC.IBILI, Portugal	Ongoing
2021/01/01 - 2024/12/31	Overcoming the major roadblocks for successful AAV-based gene therapy in the CNS 2020.09668.BD 10.54499/2020.09668.BD Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2020/06 - 2020/08	Validação expedita e Fabrico em larga escala de um dispositivo de sopro para colheita com inativação de amostras para deteção do SARS-CoV-2 269_596884125 Researcher Universidade de Coimbra, Portugal Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal ViraVector - Unidade de produção vectores virais para transferência de genes, Portugal	Fundação para a Ciência e a Tecnologia Agency for Clinical Research and Biomedical Innovation (AICIB) Ongoing

Contract

	Designation	Funders
2018/09 - Current	Targeting mutant ATXN3 for the treatment of Spinocerebellar Ataxia 3 (SCA3) CSD3#IV0020 Researcher Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal Wave Life Sciences USA, Inc., United States	Wave Life Sciences USA, Inc. Ongoing
2023/09/01 - 2026/08/31	NEURODIET - Molecular Mechanisms of Dietary Intervention on Neurodegeneration (PT001) JPND/0001/2022 10.54499/JPND/0001/2022 Universidade de Coimbra, Portugal Universidade Nova de Lisboa ¿Laboratório de Instrumentação Engenharia Biomédica e Física da Radiação, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2023/03/01 - 2024/08/31	Direct Conversion of Astrocytes into Cerebellar Neurons as an Innovative Treatment for Spinocerebellar Ataxia Type-3 2022.06127.PTDC 10.54499/2022.06127.PTDC Universidade de Coimbra, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2019/12 - 2020/01	A Therapeutic Strategy in Machado-Joseph Disease CPD0020A01 Researcher Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Blade Therapeutics Ongoing
2019/02/20 - 2019/09/30	Targeting ATXN3 for the treatment of Spinocerebellar Ataxia 3 (SCA3) using AAV5 and an artificial microRNA NA Researcher Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	uniQure NV Concluded

Other

	Designation	Funders
2020/03 - Current	Laboratório de Análises Clínicas da Universidade de Coimbra - SARS-Cov2 Detection LAC-UC Other Universidade de Coimbra, Portugal	Ongoing

Outputs

Publications

Conference poster	Lobo, Diana Duarte; Luís Quintino; Ana Carolina Silva; Carina Henriques; Ribeiro, Rodrigo; Lopes, Sara M. ; Pedro Perdigão; et al. Corresponding author: Pereira de Almeida, Luis Fernando Morgado. "CRISPR activation as a therapeutic tool for Spinocerebellar ataxia type 3 (SCA3)". Paper presented in ESGCT/SFTCG/NVGCT Collaborative Congress (Brussels, Belgium) 24-27October 2023, 2023. Henriques, Carina S.; Lopes, M. M.; Albuquerque, PR; Rufino-Ramos, David; Gaspar, Laetitia; Lobo, Diana Duarte; Duarte, Sónia; et al. "Extracellular vesicles-associated AAVs as new gene delivery vectors for the treatment of brain disorders". Paper presented in 3rd BEB Day, 2019. Henriques, Carina S.; Lopes, M. M.; Albuquerque, PR; Rufino-Ramos, David; Gaspar, Laetitia; Lobo, Diana Duarte; Duarte, Sónia; et al. "Extracellular vesicles-associated AAVs as gene delivery vectors for the treatment of brain diseases". Paper presented in 5th GEIVEX Symposium, 2019. Rui Jorge Nobre; Lobo, Diana Duarte; Catarina Oliveira Miranda; Pereira, Dina; Castelhano, Joao; Sereno, José Vitor Oliveira; Castelo-Branco, Miguel; Pereira de Almeida, Luis Fernando Morgado. "Blood-brain barrier dysfunction in spinocerebellar ataxia type 3 (Machado-Joseph disease): Results from mouse and human brain tissues". Paper presented in XVI Meeting of the Portuguese Society for Neuroscience, 2019.
Journal article	Rufino-Ramos, David; Albuquerque, Patrícia R.; Leandro, Kevin; Carmona, Vitor; Martins, Inês M.; Fernandes, Rita; Henriques, Carina; et al. "Extracellular vesicle-based delivery of silencing sequences for the treatment of Machado-Joseph disease/spinocerebellar ataxia type 3". Molecular Therapy 31 5 (2023): 1275-1292. http://dx.doi.org/10.1016/j.ymthe.2023.04.001. 10.1016/j.ymthe.2023.04.001 Nobre, Rui Jorge; Diana D. Lobo; Carina Henriques; Sonia P. Duarte; Sara M. Lopes; Ana C. Silva; Miguel M. Lopes; et al. "miRNA-Mediated Knockdown of ATXN3 Alleviates Molecular Disease Hallmarks in a Mouse Model for Spinocerebellar Ataxia Type 3". Nucleic Acid Therapeutics (2022): https://doi.org/10.1089/nat.2021.0020. 10.1089/nat.2021.0020 Nobre, Rui Jorge; Lobo, Diana Duarte; Henriques, Carina; Pereira de Almeida, Luis Fernando Morgado. Corresponding author: Pereira de Almeida, Luis Fernando Morgado. "miRNA mediated knockdown of ATXN3 alleviates molecular disease hallmarks in a mouse model for Spinocerebellar ataxia type 3". Nucleic Acid Therapeutics (2021): Accepted Vasconcelos-Ferreira, Ana; Martins, Inês Morgado; Lobo, Diana; Pereira, Dina; Lopes, Miguel M.; Faro, Rosário; Lopes, Sara M.; et al. "ULK overexpression mitigates motor deficits and neuropathology in mouse models of Machado-Joseph disease". Molecular Therapy (2021): http://dx.doi.org/10.1016/j.ymthe.2021.07.012. Published • 10.1016/j.ymthe.2021.07.012 Duarte Lobo, Diana; Nobre, Rui Jorge; Oliveira Miranda, Catarina; Pereira, Dina; Castelhano, João; Sereno, José; Koeppen, Arnulf; Castelo-Branco, Miguel; Pereira de Almeida, Luís. "The blood-brain barrier is disrupted in Machado-Joseph disease/spinocerebellar ataxia type 3: evidence from transgenic mice and human post-mortem samples". Acta Neuropathologica Communications 8 1 (2020): http://dx.doi.org/10.1186/s40478-020-00955-0. Published • 10.1186/s40478-020-00955-0 Silva, Ana C; Lobo, Diana D; Martins, Inês M; Lopes, Sara M; Henriques, Carina; Duarte, Sónia P; Dodart, Jean-Cosme; Nobre, Rui Jorge; Pereira de Almeida, Luis. "Antisense oligonucleotide therapeutics in neurodegenerative diseases: the case of polyglutamine disorders". Brain 143 2 (2019): 407-429. http://dx.doi.org/10.1093/brain/awz328. 10.1093/brain/awz328
Newspaper article	Vinhas, Ana Catarina; Ferreira, Ana Cristina; Henriques, Carina S.; Silva, Carolina; Oliveira Miranda, Catarina; Henriques, Daniel; Oliveira, Daniela; et al. "Uma viagem rara", Público, 2020, https://www.publico.pt/2020/02/28/ciencia/fotogaleria/viagem-rara-400481.
Thesis / Dissertation	Lobo, Diana Filipa Duarte. "Cerebrovascular and Blood-Brain Barrier Impairments in Machado-Joseph Disease". Master, Universidade de Coimbra Centro de Neurociências e Biologia Celular, 2017. http://hdl.handle.net/10362/25829.

Artistic / Performance

Theatre play

2019. Holy CIBB.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2019/11/15	Blood-brain barrier dysfunction as a neuropathological feature in Machado-Joseph Disease	1st Annual Meeting in Pharmaceutical Sciences Faculty of Pharmacy - University of Coimbra (Coimbra, Portugal)
2019/11/15	Extracellular vesicles-associated AAVs as gene delivery vectors for the treatment of brain disorders	1st Annual Meeting in Pharmaceutical Sciences Faculty of Pharmacy - University of Coimbra (Coimbra, Portugal)
2019/06/01	Blood-brain barrier dysfunction as a neuropathological feature in Machado-Joseph Disease: results from human and MJD mouse samples	XVI Meeting of the Portuguese Society for Neuroscience Portuguese Society for Neuroscience (Lisbon, Portugal)
2019/02/06	Extracellular vesicles-associated AAVs as gene delivery vectors for the treatment of brain diseases	XLIX Reunião da Sociedade Portuguesa de Farmacologia, XXXVII Reunião de Farmacologia clínica, XVIII Reunião de toxicologia Sociedade Portuguesa de Farmacologia, Farmacologia clínica e Toxicologia (Porto, Portugal)
2019/02	Disruption of Blood-Brain Barrier in Machado-Joseph Disease	XLIX Reunião da Sociedade Portuguesa de Farmacologia, XXXVII Reunião de Farmacologia clínica, XVIII Reunião de toxicologia Sociedade Portuguesa de Farmacologia, Farmacologia clínica e Toxicologia (Porto, Portugal)
2018/02	Cerebrovascular and Blood-Brain Barrier Impairments in Machado-Joseph Disease	XLVIII Reunião da Sociedade Portuguesa de Farmacologia, XXXVI Reunião de Farmacologia clínica, XVII Reunião de toxicologia Sociedade Portuguesa de Farmacologia, Farmacologia clínica e Toxicologia (Lisboa, Portugal)

Event participation

	Activity description Type of event	Event name Institution / Organization
2019/03 - Current	Brain Awareness Week initiative involves visits to schools to discuss neuroscience and to open the door of laboratories with the aim of disseminating science among children. Other	Science Communication - Brain Awareness Week 2019 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2022/04/27 - 2022/04/29	2022- ESGCT SPRING SCHOOL ATHENS Workshop	2022- ESGCT SPRING SCHOOL ATHENS European Society of Gene and Cell Therapy, United Kingdom

Mentoring / Tutoring

	Topic	Student name
2023/09 - 2024/09	CRISPR-based gene activation for the study and treatment of neurodegenerative disorders	Marcos Pinto

Distinctions

Award

2019	Scholarship to participate in the 2019 meeting of the Portuguese Society for Neuroscience Portuguese Society for Neuroscience, Portugal