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João Pedro Azevedo Silva. Concluiu o(a) Doutoramento em Biologia Molecular e Ambiental em 2016 pelo(a) Universidade do Minho. Publicou 12 artigos em revistas especializadas. Recebeu 3 prémio(s) e/ou homenagens. Participa e/ou participou como Bolseiro de Doutoramento em 1 projeto(s), Bolseiro de Investigação em 1 projeto(s), Bolseiro de Pós-Doutoramento em 1 projeto(s) e Investigador em 1 projeto(s).
Identification

Personal identification

Full name
João Pedro Azevedo Silva
Date of birth
1987/09/02
Gender
Male

Citation names

  • Azevedo-Silva, João

Author identifiers

Ciência ID
D81D-42AB-F4B5
ORCID iD
0000-0003-1754-9254
Google Scholar ID
https://scholar.google.pt/citations?user=B7MPYu0AAAAJ&hl=pt-PT

Knowledge fields

  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Intermediate (B1) Advanced (C1)
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2012 - 2016
Concluded
Biologia Molecular e Ambiental (Doutoramento)
Major in Especialidade: Biologia Celular e Saúde
Universidade do Minho, Portugal
"The role of monocarboxylate transporters in 3-bromopyruvate effect in cancer cells" (THESIS/DISSERTATION)
2016
Concluded
Laboratório de empresas (Outros)
TecMinho Associação Universidade-Empresa para o Desenvolvimento, Portugal
2012 - 2012
Concluded
IdeaLab – Business Ideas Laboratory (Outros)
TecMinho Associação Universidade-Empresa para o Desenvolvimento, Portugal
2008 - 2010
Concluded
Mestrado em Bioquímica (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Protein-engineered hybrid biomaterials: molecular genetic strategies for recombinant proteins expression in Escherichia coli" (THESIS/DISSERTATION)
Projects

Grant

Designation Funders
2012 - 2016 THE ROLE OF MONOCARBOXYLATE TRANSPORTERS IN 3-BROMOPYRUVATE EFFECT IN CANCER CELLS
SFRH/BD/76038/2011
PhD Student Fellow
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Concluded

Contract

Designation Funders
2019 - Current Alchemy
POCI-01-0247-FEDER-027578
Researcher
Portuguesa Centro de Biotecnologia e Química Fina, Portugal
Ongoing
2016/09 - 2019/01 Trans-acids
PTDC/BIAMIC/5184/2014
Post-doc Fellow
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Ongoing
2010/09 - 2011/12 ECOPLAST - FP7-NMP-2009-SME-3
FP7-NMP-2009-SME-3
Research Fellow
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Concluded
Outputs

Publications

Conference abstract
  1. Azevedo-Silva, João; Ribas, D.; Soares-Silva, I.; Castagnoli, D.; Sá-Pessoa, J.; Athayde, D.; Archer, M.; Casal, M.. "Exploring the function and structure of the Acetate transporter family". Paper presented in Free Radical Biology and Medicine, Lisboa, 2018.
    Published
  2. Azevedo-Silva, João; Queirós, O.; Ribeiro, A.; Baltazar, F.; Ko, Y.H.; Pedersen, P.L.; Preto, A.; Casal, M.. "The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH". Paper presented in Eur Biophys J, Dresden, 2015.
    Published
  3. Azevedo-Silva, João; Queirós, O.; Ko, Y.H.; Pedersen, P.L.; Preto, A.; Casal, M.. "Characterization of 3-bromopyruvate uptake in breast cancer cells". Paper presented in Front. Pharmacol, Munique, 2014.
    Published
  4. Collins, T.; Barroca, M.; Branca, F.; Azevedo-Silva, João; da Costa, A.; Machado, R.; Casal, M.. "Optimising a cell factory system for the bioproduction of silk-elastin-like polymers". Paper presented in 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, 2013.
    Published
  5. Machado, R.; da Costa, A.; Sencadas, V.; Azevedo-Silva, João; Collins, T.; Arias, F.; Lanceros-Mendez, S.; Rodríguez-Cabello, J.C.; Casal, M.. "Synthesis and processing of recombinant silk and elastin copolymers". Paper presented in 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, 2013.
    Published
  6. Campos, A.R.; Oliveira, B.; Machado, R.; Collins, T.; Azevedo-Silva, João; da Costa, A.; Immonen, K.; Wikström, L.; Casal, M.. "Novel nanocellulose reinforced recombinant protein based polymer composites". Paper presented in 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, 2013.
    Published
  7. Pacheco, A.; Queirós, O.; Preto, A.; Pinheiro, C.; Azevedo-Silva, João; Moreira, R.; Pedro, M.; Baltazar, F.; Casal, M.. "3-bromopyruvate cytotoxic effect in breast cancer cells is dependent of monocarboxylate transporters (MCT) expression and is enhanced by butyrate". Paper presented in Exp Clin Endocrinol Diabetes, 2012.
    Published
Conference poster
  1. Azevedo-Silva, João; Ribas, D.; Soares-Silva, I.; Castagnoli, D.; Sá-Pessoa, J.; Athayde, D.; Archer, M.; Casal, M.. "Exploring the function and structure of the Acetate transporter family". Paper presented in 19th biennial meeting for the Society for Free Radical Research International, 2018.
  2. Azevedo-Silva, João; Athayde, D.; Ribas, D.; Sá-Pessoa, J.; Archer, M.; Casal, M.. "Unravelling the structure of Acetate transporters in bacteria: expression and purification studies towards crystallography". Paper presented in XLI Jornadas Portuguesas de Genética, 2017.
  3. Granja, S.; Azevedo-Silva, João; Queirós, O.; Preto, A.; Baltazar, F.; Casal, M.. "Targeting glycolysis in acute myeloid leukemia: therapeutic applications". Paper presented in XIX Congresso Nacional de Bioquímica, 2016.
  4. Azevedo-Silva, João; Queirós, O.; Ribeiro, A.; Baltazar, F.; Ko, Y.H.; Pedersen, P.L.; Preto, A.; Casal, M.. "The extracellular pH influences the cytotoxicity and transport of 3-Bromopyruvate in breast cancer cells". Paper presented in 2nd ASPIC International Congress, 2016.
  5. Azevedo-Silva, João; Queirós, O.; Ribeiro, A.; Baltazar, F.; Ko, Y.H.; Pedersen, P.L.; Preto, A.; Casal, M.. "The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH". Paper presented in 10th European Biophysics Congress, 2015.
  6. Azevedo-Silva, João; Baltazar, F.; Ko, Y.H.; Pedersen, P.L.; Preto, A.; Casal, M.. "Silencing of Monocarboxylate transporters as a tool to study the role of 3-bromopyruvate in breast cancer". Paper presented in XXXIX Jornadas Portuguesas de Genética, 2015.
  7. Azevedo-Silva, João; Queirós, O.; Ribeiro, A.; Baltazar, F.; Ko, Y.H.; Pedersen, P.L.; Preto, A.; Casal, M.. "The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH". Paper presented in 2as Jornadas do programa doutoral em Biologia Molecular e Ambiental, 2014.
  8. Azevedo-Silva, João; Queirós, O.; Ko, Y.H.; Pedersen, P.L.; Preto, A.; Casal, M.. "Characterization of 3-bromopyruvate uptake in breast cancer cells". Paper presented in 1as Jornadas do programa doutoral em Biologia Molecular e Ambiental, 2013.
  9. Campos, A.R.; Oliveira, B.; Machado, R.; Collins, T.; Azevedo-Silva, João; da Costa, A.; Immonen, K.; Wikström, L.; Casal, M.. "Novel nanocellulose reinforced recombinante protein based polymer composites". Paper presented in 1st Internacional Conference on Natural Fibres (ICNF2013), 2013.
  10. Collins, T.; Azevedo-Silva, João; da Costa, A.; Branca, F.; Machado, R.; Casal, M.. "Key stress factors and parameters for batch production optimisation of silk-elastin-like proteins in E. coli". Paper presented in 7th Conference on Recombinant Protein Production (RPP7), 2013.
  11. Pacheco, A.; Queirós, O.; Preto, A.; Pinheiro, C.; Azevedo-Silva, João; Moreira, R.; Pedro, M.; et al. "Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate". Paper presented in XXI Porto Cancer Meeting, 2012.
  12. Collins, T.; Azevedo-Silva, João; da Costa, A.; Machado, R.; Casal, M.. "Key stress factors and parameters for batch production optimisation of silk-elastin-like proteins in E. coli". Paper presented in Microbiotec’11, 2011.
  13. Azevedo-Silva, João; da Costa, A.; Machado, R.; Rodríguez-Cabello, J.C.; Casal, M.. "Designing a cost-effective protein purification protocol using Elastin Like Polymer (VPAVG)200 fusion tag". Paper presented in XXXVI Jornadas de Genética, 2011.
  14. Azevedo-Silva, João; da Costa, A.; Machado, R.; Rodríguez-Cabello, J.C.; Casal, M.. "Use of Elastin Like Polymer (VPAVG)200 fusion tags for recombinant protein production and purification". Paper presented in XVII Congresso Nacional de Bioquímica, 2010.
  15. Azevedo-Silva, João; Machado, R.; Reis, R.L.; Rodríguez-Cabello, J.C.; Casal, M.. "Creating new functional biomaterials: construction and production of Bone Morphogenetic Protein 2-ELP hybrid protein". Paper presented in XXXV Jornadas Portuguesas de Genética, 2010.
Journal article
  1. Soares-Silva, I.; Ribas, D.; Sousa-Silva, Maria; Azevedo-Silva, João; Rendulic, T.; Margarida Paula Pedra Amorim Casal. "Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications". FEMS Microbiology Letters (2020):
    Accepted
  2. Joana Pereira-Vieira; João Azevedo-Silva; Ana Preto; Margarida Casal; Odília Queirós. "MCT1, MCT4 and CD147 expression and 3-bromopyruvate toxicity in colorectal cancer cells are modulated by the extracellular conditions". Biological Chemistry 400 6 (2019): 787-799. https://doi.org/10.1515/hsz-2018-0411.
    10.1515/hsz-2018-0411
  3. Ribas, D.; Soares-Silva, I.; Vieira, D.; Sousa-Silva, M.; Sá-Pessoa, J.; Azevedo-Silva, J.; Viegas, S.C.; et al. "The acetate uptake transporter family motif “NPAPLGL(M/S)” is essential for substrate uptake". Fungal Genetics and Biology 122 (2019): 1-10. http://www.scopus.com/inward/record.url?eid=2-s2.0-85055317896&partnerID=MN8TOARS.
    10.1016/j.fgb.2018.10.001
  4. Silva, João. "Colorectal Cancer Cells Increase the Production of Short Chain Fatty Acids by Propionibacterium freudenreichii Impacting on Cancer Cells Survival". Frontiers in Nutrition (2018): http://europepmc.org/articles/PMC5976756.
    10.3389/fnut.2018.00044
  5. Silva, João. "The Role of Diet Related Short-Chain Fatty Acids in Colorectal Cancer Metabolism and Survival: Prevention and Therapeutic Implications". Current medicinal chemistry (2018): https://doi.org/10.2174/0929867325666180530102050.
    10.2174/0929867325666180530102050
  6. Ferro S; Azevedo-Silva J; Casal M; Côrte-Real M; Baltazar F; Preto A. "Characterization of acetate transport in colorectal cancer cells and potential therapeutic implications.". (2016): http://europepmc.org/abstract/med/27661124.
    10.18632/oncotarget.12156
  7. Azevedo-Silva J; Queirós O; Baltazar F; Ulaszewski S; Goffeau A; Ko YH; Pedersen PL; Preto A; Casal M. "The anticancer agent 3-bromopyruvate: a simple but powerful molecule taken from the lab to the bedside.". (2016): http://europepmc.org/abstract/med/27457582.
    10.1007/s10863-016-9670-z
  8. Azevedo-Silva, J.Ã.; Queirós, O.; Ribeiro, A.; Baltazar, F.; Young, K.H.; Pedersen, P.L.; Pretoand, A.; Casal, M.. "The cytotoxicity of 3-bromopyruvate in breast cancer cells depends on extracellular pH". Biochemical Journal 467 2 (2015): 247-258. http://www.scopus.com/inward/record.url?eid=2-s2.0-84930804928&partnerID=MN8TOARS.
    10.1042/BJ20140921
  9. Baltazar, F.; Pinheiro, C.; Morais-Santos, F.; Azevedo-Silva, J.; Queirós, O.; Preto, A.; Casal, M.. "Monocarboxylate transporters as targets and mediators in cancer therapy response". Histology and Histopathology 29 12 (2014): 1511-1524. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907828807&partnerID=MN8TOARS.
  10. Machado, R.; Azevedo-Silva, J.; Correia, C.; Collins, T.; Arias, F.J.; Rodríguez-Cabello, J.C.; Casal, M.. "High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures". AMB Express 3 (2013): 1-15. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875063022&partnerID=MN8TOARS.
    10.1186/2191-0855-3-11
  11. Collins, T.; Azevedo-Silva, J.; da Costa, A.; Branca, F.; Machado, R.; Casal, M.. "Batch production of a silk-elastin-like protein in E. coli BL21(DE3): Key parameters for optimisation". Microbial Cell Factories 12 1 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84874275407&partnerID=MN8TOARS.
    10.1186/1475-2859-12-21
  12. Pinheiro, C.; Longatto-Filho, A.; Azevedo-Silva, J.; Casal, M.; Schmitt, F.C.; Baltazar, F.. "Role of monocarboxylate transporters in human cancers: State of the art". Journal of Bioenergetics and Biomembranes 44 1 (2012): 127-139. http://www.scopus.com/inward/record.url?eid=2-s2.0-84858705550&partnerID=MN8TOARS.
    10.1007/s10863-012-9428-1
  13. Queirós, O.; Preto, A.; Pacheco, A.; Pinheiro, C.; Azevedo-Silva, J.; Moreira, R.; Pedro, M.; et al. "Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate". Journal of Bioenergetics and Biomembranes 44 1 (2012): 141-153. http://www.scopus.com/inward/record.url?eid=2-s2.0-84858708932&partnerID=MN8TOARS.
    10.1007/s10863-012-9418-3
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2018/06 The relationship between structure and function in the Acetate transporter family XXI Jornadas de Biologia de Leveduras
CBMA (Braga, Portugal)
2016/12 Transport of short chain fatty acids and 3-bromopyruvate in cancer cells: implications for cancer therapy XIX Congresso Nacional de Bioquímica
Sociedade Portuguesa de Bioquímica (Guimarães, Portugal)
2016/08 Transport of monocarboxylic acids in cancer cells: implications of short chain fatty acids and 3-bromopyruvate for cancer therapy Small meeting on yeast transport and energetics
University of Athens (Chania, Greece)
2016/06 The extracellular pH influences the cytotoxicity and transport of 3-Bromopyruvate by monocarboxylate transporters in breast cancer cells XL Jornadas Portuguesas de Genética
Sociedade Portuguesa de Genética (Coimbra, Portugal)
2014/01 The anticancer selectivity of 3-Bromopyruvate is dependent on MCTs activity and extracellular pH: a kinetic approach DBio Seminars
CBMA (Braga, Portugal)
2013/10 Characterization of 3-bromopyruvate uptake in breast cancer cells 4th Annual Meeting of the International Society of Proton Dynamics in Cancer
International Society of Proton Dynamics in Cancer (Munique, Germany)
2010/11/03 Protein-engineered hybrid biomaterials: molecular genetic strategies for recombinant proteins expression in Escherichia coli. DBio Seminars
CBMA (Braga, Portugal)

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2017 Molecular Mechanisms Involved in 3- Bromopyruvate Effect in Colorectal Cancer Cell Lines
(Thesis) Main arguer
Joana Margarida Pereira Vieira (Master)
Universidade do Minho, Portugal

Committee member

Activity description
Role
Institution / Organization
2015 - Current Member of the scientific committee of the III Symposium of the PhD Programme on Molecular and Environmental Biology
Member
2014 - Current Member of the organizing committee of the II Symposium of the PhD Programme on Molecular and Environmental Biology
Member
2016 - 2016 Member of the organizing committee of the Portuguese national congress of Biochemistry
Member
Sociedade Portuguesa de Bioquímica, Portugal

Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
2013 - 2014 Member of the pedagogical council of School of Sciences – University of Minho (elected)
Member
Universidade do Minho Escola de Ciências, Portugal
2007 - 2008 Member of the pedagogical council of Institute of Biomedical Sciences Abel Salazar (elected)
Member
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2018 - Current Biomedicine & Pharmacotherapy (0753-3322) Elsevier
2018 - Current Yeast (1097-0061) Wiley (Blackwell Publishing)
2018 - Current Computational and Structural Biotechnology Journal (2001-0370) Elsevier
2018 - Current FEMS Yeast Research (1567-1364) Oxford University Press
Distinctions

Award

2012 Winner of the 1st Edition of ‘Ciência em Negócio’
Universidade do Minho Escola de Ciências, Portugal

Other distinction

2015 Awarded with a bursary to attend the 10th European Biophysics Congress. Dresden, July 2015.
2014 Best poster presentation in the II Symposium of the PhD Programme on Molecular and Environmental Biology
2012 Recognition by the Portuguese Society of Senology, in a tribute given to research groups in Portugal investigating breast cancer and publish in peer-reviewed journals.