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Ana Margarida Grilo Gonçalves Grilo Gonçalves is current a Biomedical phD student at University of Beira Interior. Conclude her master in Biomedical Science in 2010 and her degree in Biomedical Science in 2008 by University of Beira. Is co-founder of the enterprise inSci.Lda.
Identification

Personal identification

Full name
Ana Margarida Grilo Gonçalves

Citation names

  • Gonçalves, Ana

Author identifiers

Ciência ID
C916-15B6-8D3D
ORCID iD
0000-0001-5714-2235

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Intermediate (B1) Advanced (C1) Intermediate (B1) Intermediate (B1)
French Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2013
Concluded
 Biomedicine (Postgraduate Certificate)
Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
 18
2010
Concluded
 Biomedical Science (Master)
Universidade da Beira Interior, Portugal
"Development and Implementation of new protocols in Antibody Purification." (THESIS/DISSERTATION)
 17 valores
2008
Concluded
 Biomedical Science (Licence)
Universidade da Beira Interior, Portugal
"The use of Artificial Intelligence in Pattern Recognition with applications in Machine Learning and Adaptive Systems" (THESIS/DISSERTATION)
 13 valores
Affiliation

Others

Category
Host institution 		Employer
2018/01/18 - Current Co-founder of the company inSci inSci.Lda, Portugal
2012/09/01 - Current PhD student in Biomedicine Universidade da Beira Interior, Portugal
2017/10/01 - 2019 Member of the Pharmaco-Toxicology laboratory Universidade da Beira Interior, Portugal
2017/10/01 - 2018/05/30 Superior laboratory technique for experimental classes. Universidade da Beira Interior, Portugal
2013/05/02 - 2015/04/30 Research Fellow within the scope of the reference project CENTRO-07-ST24-FEDER-002014 Universidade da Beira Interior, Portugal
2008/09/01 - 2010/07/30 Master's student in Biomedical Sciences Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2005/09/26 - 2008/07/16 Bachelor's Degree Student in Biomedical Sciences Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
Projects

Grant

Designation Funders
2019 - 2024 Biointeration studies of membrane-bound catechol-O-methyltransferase polymorphic variants with commercial Parkinson’s inhibitors
SFRH/BD/147519/2019
PhD Student Fellow
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2013 - 2015 Technologies for Purification and controlled release of biopharmaceuticals to be applied in age-related diseases
CENTRO-07- ST24-FEDER-002014
Research Fellow
Universidade de Aveiro Centro de Investigação Didática e Tecnologia na Formação de Formadores, Portugal
Concluded

Other

Designation Funders
2020 - 2021 Design of new catechol-O-methyltransferase inhibitors with therapeutic potential for diseases of central nervous system
Centro-01-0145-FEDER-000019-C4- Centro de Competências em Cloud Computing
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Concluded
Outputs

Publications

Conference abstract
  1. Gonçalves, Ana; Maia, Cláudio J. B.; Passarinha, Luís. "Biosynthesis and isolation of STEAP1 peptides for prostate cancer immunotherapy". Paper presented in 38th FEBS congress, 2013.
    Published
Conference poster
  1. Gonçalves, Ana; Fátima M. Santos; Diogo, J.; Gallardo, Eugenia; Maia, Cláudio J. B.; Passarinha, Luís. "A new multiple reaction monitoring method for the assesment of catechol-O-methyltransferase Val/Met108". Paper presented in 10ENC em Bragança, 2017.
  2. Gonçalves, Ana; Maia, Cláudio J. B.; Passarinha, Luís. "Biosynthesis and isolation of STEAP1 peptides for prostate cancer immunotherapy". Paper presented in 38th FEBS congress, 2013.
  3. Soares, Rui; Ferreira, S.; A. Q. Pedro; Gonçalves, Ana; Maia, Cláudio J. B.; Bonifácio, MJ; Passarinha, Luís. "Biosynthesis of human membranebound catecholOmethyltransferase: optimization using PlackettBurman and Central Composite Rotatable Design". Paper presented in ESBES 9th European Symposium on Biochemical Engineering Science ISPPP 32th International Symposium on the Separation of Proteins, Peptides and Polynucleotides,, 2012.
Journal article
  1. J Barroca-Ferreira; AM Gonçalves; MFA Santos; T Santos-Silva; CJ Maia; LA Passarinha. "A chromatographic network for the purification of detergent-solubilized six-transmembrane epithelial antigen of the prostate 1 from Komagataella pastoris mini-bioreactor lysates". Journal of Chromatography A (2022): https://doi.org/10.1016/j.chroma.2022.463576.
    10.1016/j.chroma.2022.463576
  2. Neves, Ana Raquel; Albuquerque, Tânia; Faria, Rúben; Gonçalves, Ana M.; Santos, Cecília; Vivès, Eric; Boisguérin, Prisca; et al. "Development of WRAP5 Peptide Complexes for Targeted Drug/Gene Co-Delivery toward Glioblastoma Therapy". Pharmaceutics 14 10 (2022): 2213. http://dx.doi.org/10.3390/pharmaceutics14102213.
    10.3390/pharmaceutics14102213
  3. Cruz-Vicente, Pedro; Gonçalves, Ana M.; Barroca-Ferreira, Jorge; Silvestre, Samuel M.; Romão, Maria J.; Queiroz, João A.; Gallardo, Eugénia; Passarinha, Luis A.. "Unveiling the biopathway for the design of novel COMT inhibitors". Drug Discovery Today 27 10 (2022): 103328. http://dx.doi.org/10.1016/j.drudis.2022.07.013.
    10.1016/j.drudis.2022.07.013
  4. Gonçalves, Ana M.; Sousa, Ângela; Pedro, Augusto Q.; Romão, Maria J.; Queiroz, João A.; Gallardo, Eugénia; Passarinha, Luís A.. "Advances in Membrane-Bound Catechol-O-Methyltransferase Stability Achieved Using a New Ionic Liquid-Based Storage Formulation". International Journal of Molecular Sciences 23 13 (2022): 7264. http://dx.doi.org/10.3390/ijms23137264.
    10.3390/ijms23137264
  5. Cruz-Vicente, Pedro; Gonçalves, Ana M.; Ferreira, Octávio; Queiroz, João A.; Silvestre, Samuel; Passarinha, Luís A.; Gallardo, Eugenia. "Discovery of Small Molecules as Membrane-Bound Catechol-O-methyltransferase Inhibitors with Interest in Parkinson’s Disease: Pharmacophore Modeling, Molecular Docking and In Vitro Experimental Validation Studies". Pharmaceuticals 15 1 (2021): 51. http://dx.doi.org/10.3390/ph15010051.
    10.3390/ph15010051
  6. Duarte, D. R.; Barroca-Ferreira, J.; Gonçalves, A. M.; Santos, F. M.; Rocha, S. M.; Pedro, A. Q.; Maia, C. J.; Passarinha, L. A.. "Impact of glycerol feeding profiles on STEAP1 biosynthesis by Komagataella pastoris using a methanol-inducible promoter". Applied Microbiology and Biotechnology 105 11 (2021): 4635-4648. http://dx.doi.org/10.1007/s00253-021-11367-y.
    10.1007/s00253-021-11367-y
  7. Barroca-Ferreira, J.; Pais, J.P.; Santos, M.M.; Goncalves, A.M.; Gomes, I.M.; Sousa, I.; Rocha, S.M.; Passarinha, L.A.; Maia, C.J.. "Targeting STEAP1 Protein in Human Cancer: Current Trends and Future Challenges". Current Cancer Drug Targets 18 3 (2018): 222-230. http://dx.doi.org/10.2174/1568009617666170427103732.
    10.2174/1568009617666170427103732
  8. Pedro, A. Q.; Gonçalves, A. M.; Queiroz, J. A.; Passarinha, L. A.. "Purification of Histidine-Tagged Membrane-Bound Catechol-O-Methyltransferase from Detergent-Solubilized Pichia pastoris Membranes". Chromatographia 81 3 (2018): 425-434. http://dx.doi.org/10.1007/s10337-017-3453-5.
    10.1007/s10337-017-3453-5
  9. Pedro, Augusto Q; Correia, Filipa F; Santos, Fátima M; Espírito-Santo, Guilherme; Gonçalves, Ana M; Bonifácio, Maria J; Queiroz, João A; Passarinha, Luís A. "Biosynthesis and purification of histidine-tagged human soluble catechol-O-methyltransferase". Journal of Chemical Technology & Biotechnology 91 12 (2016): 3035-3044. http://dx.doi.org/10.1002/jctb.4930.
    10.1002/jctb.4930
  10. Gonçalves, Ana. "An Improved HPLC Method for Quantification of Metanephrine with Coulometric Detection". Journal of Chromatography & Separation Techniques (2014): http://omicsonline.org/open-access/an-improved-hplc-method-for-quantification-of-metanephrine-with-coulometric-detection-2157-7064.1000217.php?aid=25841.
    10.4172/2157-7064.1000217
  11. Gonçalves, A.M.; Pedro, A.Q.; Santos, F.M.; Martins, L.M.; Maia, C.J.; Queiroz, J.A.; Passarinha, L.A.. "Trends in protein-based biosensor assemblies for drug screening and pharmaceutical kinetic studies". Molecules 19 8 (2014): 12461-12485. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906708189&partnerID=MN8TOARS.
    10.3390/molecules190812461
  12. Gonçalves, A.M.; Pedro, A.Q.; Maia, C.; Sousa, F.; Queiroz, J.A.; Passarinha, L.A.. "Pichia pastoris: A recombinant microfactory for antibodies and human membrane proteins". Journal of Microbiology and Biotechnology 23 5 (2013): 587-601. http://www.scopus.com/inward/record.url?eid=2-s2.0-84877343772&partnerID=MN8TOARS.
    10.4014/jmb.1210.10063
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/12/07 New shuttle vector-based expression system to improve the biosynthesis and purification of membrane bound catechol-O-methyltransferase. Congress of Microbiology and Biotechnology 2023
Universidade da Beira Interior (Covilhã, Portugal)
2016/06/30 A dual chromatography strategy for recombinant SCOMT purification XI Annual CICS-UBI Symposium
Universidade da Beira Interior
2013/05/15 Bio(technology)engineering: The upgrade in Therapeutic Human Proteins Fields II Jornadas de Bioengenharia
Universidade da Beira Interior
2012/04/27 Recombinant DNA technology: the "Lego" game in antibody production V Jornadas sobre Tecnologia e Saúde
Instituto Politécnico da Guarda
2008/03/15 Magnetoencefalografia IV Jornadas Técnicas de Imagiologia da Universidade de Aveiro,
Universidade de Aveiro

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2015 - 2015 Construção de um aptasensor baseado na tecnologia de microfluídos para a deteção do PCA3
Supervisor
 Seminário em Biotecnologia
Universidade da Beira Interior, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2013/12/02 - 2013/12/04 8º Encontro Nacional de Cromatografia, (2013/12/02 - 2013/12/04)
Meeting (Other)
 Universidade da Beira Interior, Portugal

Committee member

Activity description
Role 		Institution / Organization
2016 - 2016 Comemorações dos 10 anos de Ciências Biomedicas
Member
 Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
Distinctions

Award

2021 Promove 3th Editions winner
BPI, Portugal

Caixabank SA, Spain