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Maria Helena Dias dos Santos, graduated in Chemical Engineering; PhD in structure/function relationship of proteins by Nuclear Magnetic Resonance (1984). Professor of Biochemistry and Head of the Cell Physiology & NMR Lab at Instituto de Tecnologia Química e Biológica (ITQB) since 1990. She was the Coordinator of two EU Projects, Coordinator of the Leaf comprising 13 partners in the EU Project “Extremophiles as Cell Factories”, BIOTECH Program, and team leader in 15 other European projects. Supervised 26 PhD theses. Main Organizer of 10 International Conferences. President of the “International Society for Extremophiles” between 2010 and 2015; Principal Investigator of the National NMR Network Project (6.5 M€) that purchased thirteen NMR spectrometers (2007). Delivered 112 invited lectures. Co-author of 6 International Patents and 241 peer-reviewed articles (WoS database). H-index 52; total citation number: 8715 (October 2020).
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Identification

Personal identification

Full name
Maria Helena Dias dos Santos

Citation names

  • Santos, Helena

Author identifiers

Ciência ID
C81C-EB4D-1E70
ORCID iD
0000-0002-8050-9485

Email addresses

  • santos@itqb.unl.pt (Professional)

Addresses

  • Instituto de Tecnologia Química e Biológica António Xavier (ITQB-NOVA), 2780-157, Oeiras, Oeiras, Portugal (Professional)
Education
Degree Classification
2000
Concluded
 Biological Sciences (Título de Agregado)
Universidade Nova de Lisboa, Portugal
1984 - 1985
Concluded
 Honorary Research Fellow (Outros)
University of Leicester, United Kingdom
1984
Concluded
 Biophysics (Doutoramento)
Universidade Nova de Lisboa, Portugal
1978
Concluded
 Inorganic Physical Chemistry (Mestrado)
Universidade Nova de Lisboa, Portugal
1968/10/01 - 1974/04/14
Concluded
 Chemical Engineering Degree (Licenciatura)
Major in Chemistry
Universidade de Lisboa Instituto Superior Técnico, Portugal
 18 out of 20
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2002/10/14 - Current Full Professor (University Teacher) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Outputs

Publications

Book
  1. Santos, H.; Lamosa, P.; Borges, N.. Characterization and Quantification of Compatible Solutes in (Hyper)thermophilic Microorganisms. 2006.
    10.1016/S0580-9517(08)70011-4
  2. Santos, H.; Da Costa, M.S.. Organic solutes from thermophiles and hyperthermophiles. 2001.
    10.1016/S0076-6879(01)34478-6
  3. Ramos, A.; Santos, H.. Nmr Studies Of Wine Chemistry And Wine Bacteria. 1999.
    10.1016/S0066-4103(08)60016-2
  4. Santos, H.; Fareleira, P.; Legall, J.; Xavier, A.V.. In vivo nuclear magnetic resonance in study of physiology of sulfate-reducing bacteria. 1994.
    10.1016/0076-6879(94)43041-1
  5. Xavier, A.V.; Turner, D.L.; Santos, H.. Two-dimensional Nuclear Magnetic Resonance of Paramagnetic Metalloproteins. 1993.
    10.1016/0076-6879(93)27003-Y
Journal article
  1. Belcher Dufrisne, Meagan; Jorge, Carla D.; Timóteo, Cristina G.; Petrou, Vasileios I.; Ashraf, Khuram U.; Banerjee, Surajit; Clarke, Oliver B.; Santos, Helena; Mancia, Filippo. "Structural and Functional Characterization of Phosphatidylinositol-Phosphate Biosynthesis in Mycobacteria". Journal of Molecular Biology 432 18 (2020): 5137-5151. http://dx.doi.org/10.1016/j.jmb.2020.04.028.
    10.1016/j.jmb.2020.04.028
  2. Hubloher, Josephine Joy; Zeidler, Sabine; Lamosa, Pedro; Santos, Helena; Averhoff, Beate; Müller, Volker. "Trehalose-6-phosphate-mediated phenotypic change in Acinetobacter baumannii". Environmental Microbiology (2020): http://dx.doi.org/10.1111/1462-2920.15148.
    10.1111/1462-2920.15148
  3. Santos, Helena. "Combined transcriptomics–metabolomics profiling of the heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus". Extremophiles (2019): http://dx.doi.org/10.1007/s00792-018-1065-0.
    10.1007/s00792-018-1065-0
  4. Gonçalves, C.; Ferreira, C.; Gonçalves, L.G.; Turner, D.L.; Leandro, M.J.; Salema-Oom, M.; Santos, H.; Gonçalves, P.. "A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation". Frontiers in Microbiology 10 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85075257813&partnerID=MN8TOARS.
    10.3389/fmicb.2019.02510
  5. Leandro, M.J.; Marques, S.; Ribeiro, B.; Santos, H.; Fonseca, C.. "Integrated process for bioenergy production and water recycling in the dairy industry: Selection of Kluyveromyces strains for direct conversion of concentrated lactose-rich streams into bioethanol". Microorganisms 7 11 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85075010356&partnerID=MN8TOARS.
    10.3390/microorganisms7110545
  6. Timmis, K.; Cavicchioli, R.; Garcia, J.L.; Nogales, B.; Chavarría, M.; Stein, L.; McGenity, T.J.; et al. "The urgent need for microbiology literacy in society". Environmental Microbiology 21 5 (2019): 1513-1528. http://www.scopus.com/inward/record.url?eid=2-s2.0-85064481965&partnerID=MN8TOARS.
    10.1111/1462-2920.14611
  7. Faria, C.; Borges, N.; Rocha, I.; Santos, H.. "Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization". Microbial cell factories 17 1 (2018): 178-178. http://www.scopus.com/inward/record.url?eid=2-s2.0-85056705726&partnerID=MN8TOARS.
    10.1186/s12934-018-1023-7
  8. Rodrigues, M.V.; Borges, N.; Santos, H.. "Glycerol phosphate cytidylyltransferase stereospecificity is key to understanding the distinct stereochemical compositions of glycerophosphoinositol in Bacteria and Archaea". Applied and Environmental Microbiology 83 1 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85009964403&partnerID=MN8TOARS.
    10.1128/AEM.02462-16
  9. Zeidler, S.; Hubloher, J.; Schabacker, K.; Lamosa, P.; Santos, H.; Müller, V.. "Trehalose, a temperature- and salt-induced solute with implications in pathobiology of Acinetobacter baumannii". Environmental Microbiology 19 12 (2017): 5088-5099. http://www.scopus.com/inward/record.url?eid=2-s2.0-85037338480&partnerID=MN8TOARS.
    10.1111/1462-2920.13987
  10. Antranikian, G.; Suleiman, M.; Schäfers, C.; Adams, M.W.W.; Bartolucci, S.; Blamey, J.M.; Birkeland, N.-K.; et al. "Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island". Extremophiles 21 4 (2017): 733-742. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019131246&partnerID=MN8TOARS.
    10.1007/s00792-017-0938-y
  11. Radoš, D.; Turner, D.L.; Catarino, T.; Hoffart, E.; Neves, A.R.; Eikmanns, B.J.; Blombach, B.; Santos, H.. "Stereospecificity of Corynebacterium glutamicum 2,3-butanediol dehydrogenase and implications for the stereochemical purity of bioproduced 2,3-butanediol". Applied Microbiology and Biotechnology 100 24 (2016): 10573-10583. http://www.scopus.com/inward/record.url?eid=2-s2.0-84989213496&partnerID=MN8TOARS.
    10.1007/s00253-016-7860-6
  12. Saraiva, M.A.; Jorge, C.D.; Santos, H.; Maçanita, A.L.. "Earliest events in a-synuclein fibrillation probed with the fluorescence of intrinsic tyrosines". Journal of Photochemistry and Photobiology B: Biology 154 (2016): 16-23. http://www.scopus.com/inward/record.url?eid=2-s2.0-84947976269&partnerID=MN8TOARS.
    10.1016/j.jphotobiol.2015.11.006
  13. Jorge, C.D.; Borges, N.; Bagyan, I.; Bilstein, A.; Santos, H.. "Potential applications of stress solutes from extremophiles in protein folding diseases and healthcare". Extremophiles 20 3 (2016): 251-259. http://www.scopus.com/inward/record.url?eid=2-s2.0-84963670607&partnerID=MN8TOARS.
    10.1007/s00792-016-0828-8
  14. Leis, B.; Angelov, A.; Mientus, M.; Li, H.; Pham, V.T.T.; Lauinger, B.; Bongen, P.; et al. "Identification of novel esterase-active enzymes from hot environments by use of the host bacterium Thermus thermophilus". Frontiers in Microbiology 6 APR (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84930942030&partnerID=MN8TOARS.
    10.3389/fmicb.2015.00275
  15. Clarke, O.B.; Tomasek, D.; Jorge, C.D.; Dufrisne, M.B.; Kim, M.; Banerjee, S.; Rajashankar, K.R.; et al. "Structural basis for phosphatidylinositol-phosphate biosynthesis". Nature Communications 6 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84974547430&partnerID=MN8TOARS.
    10.1038/ncomms9505
  16. Sand, M.; Rodrigues, M.; González, J.M.; de Crécy-Lagard, V.; Santos, H.; Müller, V.; Averhoff, B.. "Mannitol-1-phosphate dehydrogenases/phosphatases: A family of novel bifunctional enzymes for bacterial adaptation to osmotic stress". Environmental Microbiology 17 3 (2015): 711-719. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925163905&partnerID=MN8TOARS.
    10.1111/1462-2920.12503
  17. Jorge, C.D.; Borges, N.; Santos, H.. "A novel pathway for the synthesis of inositol phospholipids uses cytidine diphosphate (CDP)-inositol as donor of the polar head group". Environmental Microbiology 17 7 (2015): 2492-2504. http://www.scopus.com/inward/record.url?eid=2-s2.0-84937073217&partnerID=MN8TOARS.
    10.1111/1462-2920.12734
  18. Radoš, D.; Carvalho, A.L.; Wieschalka, S.; Neves, A.R.; Blombach, B.; Eikmanns, B.J.; Santos, H.. "Engineering Corynebacterium glutamicum for the production of 2,3-butanediol". Microbial Cell Factories 14 1 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84945891778&partnerID=MN8TOARS.
    10.1186/s12934-015-0362-x
  19. Lamosa, P.; Lourenço, E.C.; d’Avó, F.; Nobre, A.; Bandeiras, T.M.; da Costa, M.S.; Ventura, M.R.; Santos, H.. "A unique glyceryl diglycoside identified in the thermophilic, radiation-resistant bacterium Rubrobacter xylanophilus". Extremophiles 19 2 (2015): 373-382. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925532850&partnerID=MN8TOARS.
    10.1007/s00792-014-0723-0
  20. Radoš, Dušica; Turner, David L.; Fonseca, Luís L.; Carvalho, Ana Lúcia; Blombach, Bastian; Eikmanns, Bernhard J.; Neves, Ana Rute; Santos, Helena. "Carbon Flux Analysis by13C Nuclear Magnetic Resonance To Determine the Effect of CO2on Anaerobic Succinate Production by Corynebacterium glutamicum". Applied and Environmental Microbiology 80 10 (2014): 3015-3024. http://dx.doi.org/10.1128/aem.04189-13.
    10.1128/aem.04189-13
  21. Timmis, K.; de Lorenzo, V.; Verstraete, W.; Garcia, J.L.; Ramos, J.L.; Santos, H.; Economidis, I.; et al. "Pipelines for New Chemicals: A strategy to create new value chains and stimulate innovation-based economic revival in Southern European countries". Environmental Microbiology 16 1 (2014): 9-18. http://www.scopus.com/inward/record.url?eid=2-s2.0-84891594843&partnerID=MN8TOARS.
    10.1111/1462-2920.12337
  22. Esteves, A.M.; Chandrayan, S.K.; McTernan, P.M.; Borges, N.; Adams, M.W.W.; Santos, H.. "Mannosylglycerate and di-myo-inositol phosphate have interchangeable roles during adaptation of Pyrococcus furiosus to heat stress". Applied and Environmental Microbiology 80 14 (2014): 4226-4233. http://www.scopus.com/inward/record.url?eid=2-s2.0-84903182320&partnerID=MN8TOARS.
    10.1128/AEM.00559-14
  23. Borges, N.; Jorge, C.D.; Gonçalves, L.G.; Gonçalves, S.; Matias, P.M.; Santos, H.. "Mannosylglycerate: structural analysis of biosynthesis and evolutionary history". Extremophiles 18 5 (2014): 835-852. http://www.scopus.com/inward/record.url?eid=2-s2.0-84929510245&partnerID=MN8TOARS.
    10.1007/s00792-014-0661-x
  24. Shiyan, A.; Thompson, M.; Köcher, S.; Tausendschön, M.; Santos, H.; Hänelt, I.; Müller, V.. "Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in halobacillus halophilus". Frontiers in Microbiology 5 APR (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84899698704&partnerID=MN8TOARS.
    10.3389/fmicb.2014.00168
  25. Antranikian, G.; Bonch-Osmolovskaya, E.; Atomi, H.; Oren, A.; Adams, M.W.; Santos, H.. "International conference on extremophiles 2014". Extremophiles 18 5 (2014): 789-790. http://www.scopus.com/inward/record.url?eid=2-s2.0-84920376734&partnerID=MN8TOARS.
    10.1007/s00792-014-0690-5
  26. Nogly, P.; Gushchin, I.; Remeeva, A.; Esteves, A.M.; Borges, N.; Ma, P.; Ishchenko, A.; et al. "X-ray structure of a CDP-alcohol phosphatidyltransferase membrane enzyme and insights into its catalytic mechanism". Nature Communications 5 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84903147718&partnerID=MN8TOARS.
    10.1038/ncomms5169
  27. Nogly, P.; Castro, R.; De Rosa, M.; Neves, A.R.; Santos, H.; Archer, M.. "Production and crystallization of -phosphoglucomutase from Lactococcus lactis (Acta Crystallographica Section F: Structural Biology and Crystallization Communications (2012) F68 (1113-1115))". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69 11 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84887308506&partnerID=MN8TOARS.
    10.1107/S1744309113029126
  28. Carvalho, A.L.; Turner, D.L.; Fonseca, L.L.; Solopova, A.; Catarino, T.; Kuipers, O.P.; Voit, E.O.; Neves, A.R.; Santos, H.. "Metabolic and Transcriptional Analysis of Acid Stress in Lactococcus lactis, with a Focus on the Kinetics of Lactic Acid Pools". PLoS ONE 8 7 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84879753311&partnerID=MN8TOARS.
    10.1371/journal.pone.0068470
  29. Sand, M.; Mingote, A.I.; Santos, H.; Müller, V.; Averhoff, B.. "Mannitol, a compatible solute synthesized by acinetobacter baylyi in a two-step pathway including a salt-induced and salt-dependent mannitol-1-phosphate dehydrogenase". Environmental Microbiology 15 8 (2013): 2187-2197. http://www.scopus.com/inward/record.url?eid=2-s2.0-84881253330&partnerID=MN8TOARS.
    10.1111/1462-2920.12090
  30. Faria, C.; Jorge, C.D.; Borges, N.; Tenreiro, S.; Outeiro, T.F.; Santos, H.. "Inhibition of formation of a-synuclein inclusions by mannosylglycerate in a yeast model of Parkinson's disease". Biochimica et Biophysica Acta - General Subjects 1830 8 (2013): 4065-4072. http://www.scopus.com/inward/record.url?eid=2-s2.0-84877839143&partnerID=MN8TOARS.
    10.1016/j.bbagen.2013.04.015
  31. Lamosa, P.; Rodrigues, M.V.; Gonçalves, L.G.; Carr, J.; Ventura, R.; Maycock, C.; Raven, N.D.; Santos, H.. "Organic solutes in the deepest phylogenetic branches of the Bacteria: Identification of a(1-6)glucosyl-a(1-2)glucosylglycerate in Persephonella marina". Extremophiles 17 1 (2013): 137-146. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871462775&partnerID=MN8TOARS.
    10.1007/s00792-012-0500-x
  32. Gaspar, P.; Carvalho, A.L.; Vinga, S.; Santos, H.; Neves, A.R.. "From physiology to systems metabolic engineering for the production of biochemicals by lactic acid bacteria". Biotechnology Advances 31 6 (2013): 764-788. http://www.scopus.com/inward/record.url?eid=2-s2.0-84882723516&partnerID=MN8TOARS.
    10.1016/j.biotechadv.2013.03.011
  33. Nogly, P.; Matias, P.M.; De Rosa, M.; Castro, R.; Santos, H.; Neves, A.R.; Archer, M.. "High-resolution structure of an atypical -phosphoglucomutase related to eukaryotic phosphomannomutases". Acta Crystallographica Section D: Biological Crystallography 69 10 (2013): 2008-2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885458762&partnerID=MN8TOARS.
    10.1107/S0907444913017046
  34. Fonseca, C.P.; Fonseca, L.L.; Montezinho, L.P.; Alves, P.M.; Santos, H.; Castro, M.M.C.A.; Geraldes, C.F.G.C.. "23Na multiple quantum filtered NMR characterisation of Na+ binding and dynamics in animal cells: A comparative study and effect of Na+/Li+ competition". European Biophysics Journal 42 7 (2013): 503-519. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878967029&partnerID=MN8TOARS.
    10.1007/s00249-013-0899-8
  35. Pais, T.M.; Lamosa, P.; Matzapetakis, M.; Turner, D.L.; Santos, H.. "Mannosylglycerate stabilizes staphylococcal nuclease with restriction of slow ß-sheet motions". Protein Science 21 8 (2012): 1126-1137. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863795258&partnerID=MN8TOARS.
    10.1002/pro.2100
  36. Nogly, P.; Castro, R.; De Rosa, M.; Neves, A.R.; Santos, H.; Archer, M.. "Production and crystallization of a-phosphoglucomutase from Lactococcus lactis". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 68 9 (2012): 1113-1115. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865734467&partnerID=MN8TOARS.
    10.1107/S1744309112032629
  37. Santos, H.. "António Xavier and his contribution to the development of Bioinorganic Chemistry". FEBS Letters 586 5 (2012): 476-478. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857913926&partnerID=MN8TOARS.
    10.1016/j.febslet.2012.02.007
  38. Rodrigues, F.; Sousa, M.J.; Ludovico, P.; Santos, H.; Côrte-Real, M.; Leão, C.. "The Fate of Acetic Acid during Glucose Co-Metabolism by the Spoilage Yeast Zygosaccharomyces bailii". PLoS ONE 7 12 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84871689348&partnerID=MN8TOARS.
    10.1371/journal.pone.0052402
  39. Gonçalves, L.G.; Borges, N.; Serra, F.; Fernandes, P.L.; Dopazo, H.; Santos, H.. "Evolution of the biosynthesis of di-myo-inositol phosphate, a marker of adaptation to hot marine environments". Environmental Microbiology 14 3 (2012): 691-701. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857855641&partnerID=MN8TOARS.
    10.1111/j.1462-2920.2011.02621.x
  40. Gaspar, P.; Neves, A.R.; Gasson, M.J.; Shearman, C.A.; Santos, H.. "High yields of 2,3-butanediol and mannitol in Lactococcus lactis through engineering of NAD + cofactor recycling". Applied and Environmental Microbiology 77 19 (2011): 6826-6835. http://www.scopus.com/inward/record.url?eid=2-s2.0-82955178507&partnerID=MN8TOARS.
    10.1128/AEM.05544-11
  41. Sand, M.; De Berardinis, V.; Mingote, A.; Santos, H.; Göttig, S.; Müller, V.; Averhoff, B.. "Salt adaptation in Acinetobacter baylyi: IdentiWcation and characterization of a secondary glycine betaine transporter". Archives of Microbiology 193 10 (2011): 723-730. http://www.scopus.com/inward/record.url?eid=2-s2.0-82455188096&partnerID=MN8TOARS.
    10.1007/s00203-011-0713-x
  42. Brito, J.A.; Borges, N.; Vonrhein, C.; Santos, H.; Archer, M.. "Crystal structure of archaeoglobus fulgidus CTP:Inositol-1-phosphate cytidylyltransferase, a key enzyme for di-myo-inositol-phosphate synthesis in (hyper)thermophiles". Journal of Bacteriology 193 9 (2011): 2177-2185. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955532914&partnerID=MN8TOARS.
    10.1128/JB.01543-10
  43. Jorge, C.D.; Ventura, R.; Maycock, C.; Outeiro, T.F.; Santos, H.; Costa, J.. "Assessment of the efficacy of solutes from extremophiles on protein aggregation in cell models of Huntington's and Parkinson's diseases". Neurochemical Research 36 6 (2011): 1005-1011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955681334&partnerID=MN8TOARS.
    10.1007/s11064-011-0440-3
  44. Lamosa, P.; Mingote, A.I.; Groudieva, T.; Klippel, B.; Egorova, K.; Jabbour, D.; Santos, H.; Antranikian, G.. "Gluconeotrehalose is the principal organic solute in the psychrotolerant bacterium Carnobacterium strain 17-4". Extremophiles 15 4 (2011): 463-472. http://www.scopus.com/inward/record.url?eid=2-s2.0-85027948481&partnerID=MN8TOARS.
    10.1007/s00792-011-0377-0
  45. Fonseca, L.L.; Sánchez, C.; Santos, H.; Voit, E.O.. "Complex coordination of multi-scale cellular responses to environmental stress". Molecular BioSystems 7 3 (2011): 731-741. http://www.scopus.com/inward/record.url?eid=2-s2.0-79951601720&partnerID=MN8TOARS.
    10.1039/c0mb00102c
  46. Müller, S.; Hoffmann, T.; Santos, H.; Saum, S.H.; Bremer, E.; Müller, V.. "Bacterial abl-like genes: Production of the archaeal osmolyte N e-acetyl-B-lysine by homologous overexpression of the yodP-kamA genes in Bacillus subtilis". Applied Microbiology and Biotechnology 91 3 (2011): 689-697. http://www.scopus.com/inward/record.url?eid=2-s2.0-79960721717&partnerID=MN8TOARS.
    10.1007/s00253-011-3301-8
  47. Carvalho, A.L.; Cardoso, F.S.; Bohn, A.; Neves, A.R.; Santos, H.. "Engineering trehalose synthesis in Lactococcus lactis for improved stress tolerance". Applied and Environmental Microbiology 77 12 (2011): 4189-4199. http://www.scopus.com/inward/record.url?eid=2-s2.0-79960083590&partnerID=MN8TOARS.
    10.1128/AEM.02922-10
  48. Ndukum, J.; Fonseca, L.L.; Santos, H.; Voit, E.O.; Datta, S.. "Statistical inference methods for sparse biological time series data". BMC Systems Biology 5 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79954987899&partnerID=MN8TOARS.
    10.1186/1752-0509-5-57
  49. Gonçalves, S.; Esteves, A.M.; Santos, H.; Borges, N.; Matias, P.M.. "Three-dimensional structure of mannosyl-3-phosphoglycerate phosphatase from thermus thermophilus HB27: A new member of the haloalcanoic acid dehalogenase superfamily". Biochemistry 50 44 (2011): 9551-9567. http://www.scopus.com/inward/record.url?eid=2-s2.0-80155206893&partnerID=MN8TOARS.
    10.1021/bi201171h
  50. Gonçalves, S.; Esteves, A.M.; Borges, N.; Santos, H.; Matias, P.M.. "Crystallization and preliminary X-ray analysis of mannosyl-3- phosphoglycerate phosphatase from Thermus thermophilus HB27". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 3 (2011): 390-396. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952692197&partnerID=MN8TOARS.
    10.1107/S1744309111002843
  51. Collins, Tony; Matzapetakis, Manolis; Santos, Helena. "Backbone and side chain 1H, 15N and 13C assignments for a thiol-disulphide oxidoreductase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125". Biomolecular NMR Assignments 4 2 (2010): 151-154. http://dx.doi.org/10.1007/s12104-010-9230-0.
    10.1007/s12104-010-9230-0
  52. Vinga, S.; Neves, A.R.; Santos, H.; Brandt, B.W.; Kooijman, S.A.L.M.. "Subcellular metabolic organization in the context of dynamic energy budget and biochemical systems theories". Philosophical Transactions of the Royal Society B: Biological Sciences 365 1557 (2010): 3429-3442. http://www.scopus.com/inward/record.url?eid=2-s2.0-78149277848&partnerID=MN8TOARS.
    10.1098/rstb.2010.0156
  53. Gonçalves, S.; Borges, N.; Esteves, A.M.; Victor, B.L.; Soares, C.M.; Santos, H.; Matias, P.M.. "Structural analysis of Thermus thermophilus HB27 mannosyl-3- phosphoglycerate synthase provides evidence for a second catalytic metal ion and new insight into the retaining mechanism of glycosyltransferases". Journal of Biological Chemistry 285 23 (2010): 17857-17868. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952938697&partnerID=MN8TOARS.
    10.1074/jbc.M109.095976
  54. Brito, J.A.; Borges, N.; Santos, H.; Archer, M.. "Production, crystallization and preliminary X-ray analysis of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 66 11 (2010): 1463-1465. http://www.scopus.com/inward/record.url?eid=2-s2.0-78149345319&partnerID=MN8TOARS.
    10.1107/S1744309110032677
  55. Neves, A.R.; Pool, W.A.; Solopova, A.; Kok, J.; Santos, H.; Kuipers, O.P.. "Towards enhanced galactose utilization by lactococcus lactis". Applied and Environmental Microbiology 76 21 (2010): 7048-7060. http://www.scopus.com/inward/record.url?eid=2-s2.0-78149459214&partnerID=MN8TOARS.
    10.1128/AEM.01195-10
  56. Fernandes, C.; Mendes, V.; Costa, J.; Empadinhas, N.; Jorge, C.; Lamosa, P.; Santos, H.; Da Costa, M.S.. "Two alternative pathways for the synthesis of the rare compatible solute mannosylglucosylglycerate in Petrotoga mobilis". Journal of Bacteriology 192 6 (2010): 1624-1633. http://www.scopus.com/inward/record.url?eid=2-s2.0-77949416752&partnerID=MN8TOARS.
    10.1128/JB.01424-09
  57. Borges, N.; Matsumi, R.; Imanaka, T.; Atomi, H.; Santos, H.. "Thermococcus kodakarensis mutants deficient in di-myo-inositol phosphate use aspartate to cope with heat stress". Journal of Bacteriology 192 1 (2010): 191-197. http://www.scopus.com/inward/record.url?eid=2-s2.0-73849124968&partnerID=MN8TOARS.
    10.1128/JB.01115-09
  58. Vyrides, I.; Santos, H.; Mingote, A.; Ray, M.J.; Stuckey, D.C.. "Are compatible solutes compatible with biological treatment of saline wastewater? Batch and continuous studies using submerged anaerobic membrane bioreactors (SAMBRs)". Environmental Science and Technology 44 19 (2010): 7437-7442. http://www.scopus.com/inward/record.url?eid=2-s2.0-77957326887&partnerID=MN8TOARS.
    10.1021/es903981k
  59. Noronha, M.; Gerbelová, H.; Faria, T.Q.; Lund, D.N.; Smith, D.A.; Santos, H.; MaçAnita, A.L.. "Thermal unfolding kinetics of ubiquitin in the microsecond-to-second time range probed by Tyr-59 fluorescence". Journal of Physical Chemistry B 114 30 (2010): 9912-9919. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955444568&partnerID=MN8TOARS.
    10.1021/jp104167h
  60. Saum, R.; Mingote, A.; Santos, H.; Müller, V.. "A novel limb in the osmoregulatory network of Methanosarcina mazei Gö1: N-acetyl-ß-lysine can be substituted by glutamate and alanine". Environmental Microbiology 11 5 (2009): 1056-1065. http://www.scopus.com/inward/record.url?eid=2-s2.0-65349191175&partnerID=MN8TOARS.
    10.1111/j.1462-2920.2008.01826.x
  61. Rodrigues, M.V.; Borges, N.; Almeida, C.P.; Lamosa, P.; Santos, H.. "A unique ß-1,2-mannosyltransferase of Thermotoga maritima that uses di-myo-inositol phosphate as the mannosyl acceptor". Journal of Bacteriology 191 19 (2009): 6105-6115. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349096860&partnerID=MN8TOARS.
    10.1128/JB.00598-09
  62. Noronha, M.; Santos, R.; Paci, E.; Santos, H.; Maçanita, A.L.. "Fluorescence Lifetimes of Tyrosine Residues in Cytochromec'' as Local Probes to Study Protein Unfolding". Journal of Physical Chemistry B 113 13 (2009): 4466-4474. http://www.scopus.com/inward/record.url?eid=2-s2.0-65249133549&partnerID=MN8TOARS.
    10.1021/jp805781r
  63. Noronha, M.; Gerbelová, H.; Faria, T.Q.; Sampaio, M.M.; Rudolph, R.; Maçanita, A.L.; Santos, H.. "Enhancing the fluorescence of tyr-59 in ubiquitin by blocking proton transfer". Physical Chemistry Chemical Physics 11 18 (2009): 3580-3583. http://www.scopus.com/inward/record.url?eid=2-s2.0-65349155168&partnerID=MN8TOARS.
    10.1039/b819365g
  64. Gonçalves, S.; Borges, N.; Santos, H.; Matias, P.M.. "Crystallization and preliminary x-ray analysis of mannosyl-3- phosphoglycerate synthase from thermus thermophilus HB27". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65 10 (2009): 1014-1017. http://www.scopus.com/inward/record.url?eid=2-s2.0-70350312647&partnerID=MN8TOARS.
    10.1107/S1744309109032576
  65. Castro, R.; Neves, A.R.; Fonseca, L.L.; Pool, W.A.; Kok, J.; Kuipers, O.P.; Santos, H.. "Characterization of the individual glucose uptake systems of Lactococcus lactis: Mannose-PTS, cellobiose-PTS and the novel GlcU permease". Molecular Microbiology 71 3 (2009): 795-806. http://www.scopus.com/inward/record.url?eid=2-s2.0-58449116696&partnerID=MN8TOARS.
    10.1111/j.1365-2958.2008.06564.x
  66. Andersen, A.Z.; Carvalho, A.L.; Neves, A.R.; Santos, H.; Kummer, U.; Olsen, L.F.. "The metabolic pH response in Lactococcus lactis: An integrative experimental and modelling approach". Computational Biology and Chemistry 33 1 (2009): 71-83. http://www.scopus.com/inward/record.url?eid=2-s2.0-57749192247&partnerID=MN8TOARS.
    10.1016/j.compbiolchem.2008.08.001
  67. Saum, R.; Mingote, A.; Santos, H.; Müller, V.. "Genetic analysis of the role of the ABC transporter Ota and Otb in glycine betaine transport in Methanosarcina mazei Gö1". Archives of Microbiology 191 4 (2009): 191-301. http://www.scopus.com/inward/record.url?eid=2-s2.0-63149198337&partnerID=MN8TOARS.
    10.1007/s00203-008-0449-4
  68. Pais, T.M.; Lamosa, P.; Garcia-Moreno, B.; Turner, D.L.; Santos, H.. "Relationship between Protein Stabilization and Protein Rigidification Induced by Mannosylglycerate". Journal of Molecular Biology 394 2 (2009): 237-250. http://www.scopus.com/inward/record.url?eid=2-s2.0-70350523594&partnerID=MN8TOARS.
    10.1016/j.jmb.2009.09.012
  69. Sánchez, C.; Neves, A.R.; Cavalheiro, J.; Dos Santos, M.M.; García-Quintáns, N.; López, P.; Santos, H.. "Contribution of citrate metabolism to the growth of Lactococcus lactis at low pH". Applied and Environmental Microbiology 74 4 (2008): 1136-1144. http://www.scopus.com/inward/record.url?eid=2-s2.0-39649104599&partnerID=MN8TOARS.
    10.1128/AEM.01061-07
  70. Faria, T.Q.; Mingote, A.; Siopa, F.; Ventura, R.; Maycock, C.; Santos, H.. "Design of new enzyme stabilizers inspired by glycosides of hyperthermophilic microorganisms". Carbohydrate Research 343 18 (2008): 3025-3033. http://www.scopus.com/inward/record.url?eid=2-s2.0-55049115053&partnerID=MN8TOARS.
    10.1016/j.carres.2008.08.030
  71. Gonçalves, L.G.; Lamosa, P.; Huber, R.; Santos, H.. "Di-myo-inositol phosphate and novel UDP-sugars accumulate in the extreme hyperthermophile Pyrolobus fumarii". Extremophiles 12 3 (2008): 383-389. http://www.scopus.com/inward/record.url?eid=2-s2.0-43049160683&partnerID=MN8TOARS.
    10.1007/s00792-008-0143-0
  72. Fonseca, C.; Neves, A.R.; Antunes, A.M.M.; Noronha, J.P.; Hahn-Hägerdal, B.; Santos, H.; Spencer-Martins, I.. "Use of In Vivo 13C Nuclear Magnetic Resonance Spectroscopy To Elucidate l-Arabinose Metabolism in Yeasts". Applied and Environmental Microbiology 74 6 (2008): 1845-1855. http://www.scopus.com/inward/record.url?eid=2-s2.0-40849139270&partnerID=MN8TOARS.
    10.1128/AEM.02453-07
  73. Jorge, C.D.; Fonseca, L.L.; Boos, W.; Santos, H.. "Role of periplasmic trehalase in uptake of trehalose by the thermophilic bacterium Rhodothermus marinus". Journal of Bacteriology 190 6 (2008): 1871-1878. http://www.scopus.com/inward/record.url?eid=2-s2.0-40449122898&partnerID=MN8TOARS.
    10.1128/JB.01616-07
  74. Santos, Filipe; Vera, José L.; Lamosa, Pedro; de Valdez, Graciela F.; de Vos, Willem M.; Santos, Helena; Sesma, Fernando; Hugenholtz, Jeroen. "Pseudovitamin B12 is the corrinoid produced byLactobacillus reuteriCRL1098 under anaerobic conditions". FEBS Letters 581 25 (2007): 4865-4870. http://dx.doi.org/10.1016/j.febslet.2007.09.012.
    10.1016/j.febslet.2007.09.012
  75. Jorge, Carla D.; Lamosa, Pedro; Santos, Helena. "The synthesis of a trisaccharide and a tetrasaccharide lipid intermediate. P1-dolichyl P2-[O-ß-d-mannopyranosyl-(1¿4)-O-(2-acetamido-2-deoxy-ß-d-glucopyranosyl)- (1¿4)-2-acetamido-2-deoxy-a-d-glucopyranosyl] diphosphate and P1-dolichyl P2-[O-a-d-mannopyranosyl-(1¿3)-O-ß-d-mannopyranosyl-(1¿4)-O-(2-acetamido-2-deoxy-ß-d- glucopyranosyl)-(1¿4)-2-acetamido-2-deoxy-a-d-glucopyranosyl] diphosphate". FEBS Journal 274 12 (2007): 3120-3127. http://dx.doi.org/10.1111/j.1742-4658.2007.05844.x.
    10.1111/j.1742-4658.2007.05844.x
  76. Jorge, Carla D.; Lamosa, Pedro; Santos, Helena. "a-d-Mannopyranosyl-(1¿2)-a-d-glucopyranosyl-(1¿2)-glycerate in the thermophilic bacterium Petrotoga miotherma - structure, cellular content and function". FEBS Journal 274 12 (2007): 3120-3127. http://dx.doi.org/10.1111/j.1742-4658.2007.05844.x.
    10.1111/j.1742-4658.2007.05844.x
  77. Egorova, K.; Grudieva, T.; Morinez, C.; Kube, J.; Santos, H.; Da Costa, M.S.; Antranikian, G.. "High yield of mannosylglycerate production by upshock fermentation and bacterial milking of trehalose-deficient mutant Thermus thermophilus RQ-1". Applied Microbiology and Biotechnology 75 5 (2007): 1039-1045. http://www.scopus.com/inward/record.url?eid=2-s2.0-34250641980&partnerID=MN8TOARS.
    10.1007/s00253-007-0915-y
  78. Noronha, M.; Lima, J.C.; Paci, E.; Santos, H.; Maçanita, A.L.. "Tracking local conformational changes of ribonuclease A using picosecond time-resolved fluorescence of the six tyrosine residues". Biophysical Journal 92 12 (2007): 4401-4414. http://www.scopus.com/inward/record.url?eid=2-s2.0-34250366274&partnerID=MN8TOARS.
    10.1529/biophysj.106.093625
  79. Lemos, P.C.; Dai, Y.; Yuan, Z.; Keller, J.; Santos, H.; Reis, M.A.M.. "Elucidation of metabolic pathways in glycogen-accumulating organisms with in vivo 13C nuclear magnetic resonance". Environmental Microbiology 9 11 (2007): 2694-2706. http://www.scopus.com/inward/record.url?eid=2-s2.0-35148885587&partnerID=MN8TOARS.
    10.1111/j.1462-2920.2007.01382.x
  80. Cardoso, F.S.; Castro, R.F.; Borges, N.; Santos, H.. "Biochemical and genetic characterization of the pathways for trehalose metabolism in Propionibacterium freudenreichii, and their role in stress response". Microbiology 153 1 (2007): 270-280. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846305426&partnerID=MN8TOARS.
    10.1099/mic.0.29262-0
  81. Jorge, C.D.; Sampaio, M.M.; Hreggvidsson, G.Ó.; Kristjánson, J.K.; Santos, H.. "A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus". Extremophiles 11 1 (2007): 115-122. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846145742&partnerID=MN8TOARS.
    10.1007/s00792-006-0021-6
  82. Gaspar, P.; Neves, A.R.; Shearman, C.A.; Gasson, M.J.; Baptista, A.M.; Turner, D.L.; Soares, C.M.; Santos, H.. "The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties - Comparative modeling to probe the molecular basis". FEBS Journal 274 22 (2007): 5924-5936. http://www.scopus.com/inward/record.url?eid=2-s2.0-35748951589&partnerID=MN8TOARS.
    10.1111/j.1742-4658.2007.06115.x
  83. Vilela, M.; Borges, C.C.H.; Vinga, S.; Vasconcelos, A.T.R.; Santos, H.; Voit, E.O.; Almeida, J.S.. "Automated smoother for the numerical decoupling of dynamics models". BMC Bioinformatics 8 (2007): http://www.scopus.com/inward/record.url?eid=2-s2.0-35448979185&partnerID=MN8TOARS.
    10.1186/1471-2105-8-305
  84. Empadinhas, N.; Mendes, V.; Simões, C.; Santos, M.S.; Mingote, A.; Lamosa, P.; Santos, H.; Costa, M.S.D.. "Organic solutes in Rubrobacter xylanophilus: The first example of di-myo-inositol-phosphate in a thermophile". Extremophiles 11 5 (2007): 667-673. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548303944&partnerID=MN8TOARS.
    10.1007/s00792-007-0084-z
  85. Rodrigues, M.V.; Borges, N.; Henriques, M.; Lamosa, P.; Ventura, R.; Fernandes, C.; Empadinhas, N.; et al. "Bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol: Inositol-1-phosphate transferase, the key enzyme for di-myo-inositol-phosphate synthesis in several (hyper)thermophiles". Journal of Bacteriology 189 15 (2007): 5405-5412. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547629796&partnerID=MN8TOARS.
    10.1128/JB.00465-07
  86. Neves, Ana R.; Pool, Wietske A.; Castro, Rute; Mingote, Ana; Santos, Filipe; Kok, Jan; Kuipers, Oscar P.; Santos, Helena. "The a-Phosphoglucomutase of Lactococcus lactis Is Unrelated to the a-d-Phosphohexomutase Superfamily and Is Encoded by the Essential GenepgmH". Journal of Biological Chemistry 281 48 (2006): 36864-36873. http://dx.doi.org/10.1074/jbc.m607044200.
    10.1074/jbc.m607044200
  87. Passarinho, J.A.P.; Lamosa, P.; Baeta, J.P.; Santos, H.; Ricardo, C.P.P.. "Annual changes in the concentration of minerals and organic compounds of Quercus suber leaves". Physiologia Plantarum 127 1 (2006): 100-110. http://www.scopus.com/inward/record.url?eid=2-s2.0-33645886026&partnerID=MN8TOARS.
    10.1111/j.1399-3054.2006.00655.x
  88. Lamosa, P.; Gonçalves, L.G.; Rodrigues, M.V.; Martins, L.O.; Raven, N.D.H.; Santos, H.. "Occurrence of 1-glyceryl-1-myo-inosityl phosphate in hyperthermophiles". Applied and Environmental Microbiology 72 9 (2006): 6169-6173. http://www.scopus.com/inward/record.url?eid=2-s2.0-33748803006&partnerID=MN8TOARS.
    10.1128/AEM.00852-06
  89. Voit, E.O.; Almeida, J.; Marino, S.; Lall, R.; Goel, G.; Neves, A.R.; Santos, H.. "Regulation of glycolysis in Lactococcus lactis: An unfinished systems biological case study". IEE Proceedings: Systems Biology 153 4 (2006): 286-298. http://www.scopus.com/inward/record.url?eid=2-s2.0-33746615693&partnerID=MN8TOARS.
    10.1049/ip-syb:20050087
  90. Costa, J.; Empadinhas, N.; Gonçalves, L.; Lamosa, P.; Santos, H.; Da Costa, M.S.. "Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii". Journal of Bacteriology 188 3 (2006): 1022-1030. http://www.scopus.com/inward/record.url?eid=2-s2.0-31344453995&partnerID=MN8TOARS.
    10.1128/JB.188.3.1022-1030.2006
  91. Pool, W.A.; Neves, A.R.; Kok, J.; Santos, H.; Kuipers, O.P.. "Natural sweetening of food products by engineering Lactococcus lactis for glucose production". Metabolic Engineering 8 5 (2006): 456-464. http://www.scopus.com/inward/record.url?eid=2-s2.0-33747762382&partnerID=MN8TOARS.
    10.1016/j.ymben.2006.05.003
  92. Borges, N.; Gonçalves, L.G.; Rodrigues, M.V.; Siopa, F.; Ventura, R.; Maycock, C.; Lamosa, P.; Santos, H.. "Biosynthetic pathways of inositol and glycerol phosphodiesters used by the hyperthermophile Archaeoglobus fulgidus in stress adaptation". Journal of Bacteriology 188 23 (2006): 8128-8135. http://www.scopus.com/inward/record.url?eid=2-s2.0-33751559039&partnerID=MN8TOARS.
    10.1128/JB.01129-06
  93. Enguita, F.J.; Pohl, E.; Turner, D.L.; Santos, H.; Carrondo, M.A.. "Structural evidence for a proton transfer pathway coupled with haem reduction of cytochrome c'' from Methylophilus methylotrophus". Journal of Biological Inorganic Chemistry 11 2 (2006): 189-196. http://www.scopus.com/inward/record.url?eid=2-s2.0-32444435237&partnerID=MN8TOARS.
    10.1007/s00775-005-0065-6
  94. Voit, E.; Neves, A.R.; Santos, H.. "The intricate side of systyems biology". Proceedings of the National Academy of Sciences of the United States of America 103 25 (2006): 9452-9457. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745448269&partnerID=MN8TOARS.
    10.1073/pnas.0603337103
  95. Neves, C.; Da Costa, M.S.; Santos, H.. "Compatible solutes of the hyperthermophile Palaeococcus ferrophilus: Osmoadaptation and thermoadaptation in the order Thermococcales". Applied and Environmental Microbiology 71 12 (2005): 8091-8098. http://www.scopus.com/inward/record.url?eid=2-s2.0-29144472059&partnerID=MN8TOARS.
    10.1128/AEM.71.12.8091-8098.2005
  96. Neves, A.R.; Pool, W.A.; Kok, J.; Kuipers, O.P.; Santos, H.. "Overview on sugar metabolism and its control in Lactococcus lactis - The input from in vivo NMR". FEMS Microbiology Reviews 29 3 SPEC. IS (2005): 531-554. http://www.scopus.com/inward/record.url?eid=2-s2.0-23944486891&partnerID=MN8TOARS.
    10.1016/j.femsre.2005.04.005
  97. Alarico, S.; Empadinhas, N.; Simões, C.; Silva, Z.; Henne, A.; Mingote, A.; Santos, H.; Da Costa, M.S.. "Distribution of genes for synthesis of trehalose and mannosylglycerate in Thermus spp. and direct correlation of these genes with halotolerance". Applied and Environmental Microbiology 71 5 (2005): 2460-2466. http://www.scopus.com/inward/record.url?eid=2-s2.0-18444382346&partnerID=MN8TOARS.
    10.1128/AEM.71.5.2460-2466.2005
  98. Pais, T.M.; Lamosa, P.; Dos Santos, W.; Legall, J.; Turner, D.L.; Santos, H.. "Structural determinants of protein stabilization by solutes: The importance of the hairpin loop in rubredoxins". FEBS Journal 272 4 (2005): 999-1011. http://www.scopus.com/inward/record.url?eid=2-s2.0-14044252066&partnerID=MN8TOARS.
    10.1111/j.1742-4658.2004.04534.x
  99. Fonseca, L.L.; Monteiro, M.A.R.; Alves, P.M.; Carrondo, M.J.T.; Santos, H.. "Cultures of rat astrocytes challenged with a steady supply of glutamate: New model to study flux distribution in the glutamate-glutamine cycle". GLIA 51 4 (2005): 286-296. http://www.scopus.com/inward/record.url?eid=2-s2.0-25444479697&partnerID=MN8TOARS.
    10.1002/glia.20209
  100. Müller, V.; Spanheimer, R.; Santos, H.. "Stress response by solute accumulation in archaea". Current Opinion in Microbiology 8 6 (2005): 729-736. http://www.scopus.com/inward/record.url?eid=2-s2.0-27844510004&partnerID=MN8TOARS.
    10.1016/j.mib.2005.10.011
  101. Silva, Z.; Sampaio, M.-M.; Henne, A.; Böhm, A.; Gutzat, R.; Boos, W.; Da Costa, M.S.; Santos, H.. "The high-affinity maltose/trehalose ABC transporter in the extremely thermophilic bacterium Thermus thermophilus HB27 also recognizes sucrose and palatinose". Journal of Bacteriology 187 4 (2005): 1210-1218. http://www.scopus.com/inward/record.url?eid=2-s2.0-14544285469&partnerID=MN8TOARS.
    10.1128/JB.187.4.1210-1218.2005
  102. Wiame, E.; Lamosa, P.; Santos, H.; Van Schaftingen, E.. "Identification of glucoselysine-6-phosphate deglycase, an enzyme involved in the metabolism of the fructation product glucoselysine". Biochemical Journal 392 2 (2005): 263-269. http://www.scopus.com/inward/record.url?eid=2-s2.0-29144503142&partnerID=MN8TOARS.
    10.1042/BJ20051183
  103. Gaspar, P.; Neves, A.R.; Ramos, A.; Gasson, M.J.; Shearman, C.A.; Santos, H.. "Engineering Lactococcus lactis for Production of Mannitol: High Yields from Food-Grade Strains Deficient in Lactate Dehydrogenase and the Mannitol Transport System". Applied and Environmental Microbiology 70 3 (2004): 1466-1474. http://www.scopus.com/inward/record.url?eid=2-s2.0-1642296584&partnerID=MN8TOARS.
    10.1128/AEM.70.3.1466-1474.2004
  104. Cardoso, F.S.; Gaspar, P.; Hugenholtz, J.; Ramos, A.; Santos, H.. "Enhancement of trehalose production in dairy propionibacteria through manipulation of environmental conditions". International Journal of Food Microbiology 91 2 (2004): 195-204. http://www.scopus.com/inward/record.url?eid=2-s2.0-1242270620&partnerID=MN8TOARS.
    10.1016/S0168-1605(03)00387-8
  105. Noronha, M.; Lima, J.C.; Lamosa, P.; Santos, H.; Maycock, C.; Ventura, R.; Maçanita, A.L.. "Intramolecular Fluorescence Quenching of Tyrosine by the Peptide a-Carbonyl Group Revisited". Journal of Physical Chemistry A 108 12 (2004): 2155-2166. http://www.scopus.com/inward/record.url?eid=2-s2.0-1842788226&partnerID=MN8TOARS.
    10.1021/jp037125l
  106. Ramos, A.; Neves, A.R.; Ventura, R.; Maycock, C.; López, P.; Santos, H.. "Effect of pyruvate kinase overproduction on glucose metabolism of Lactococcus lactis". Microbiology 150 4 (2004): 1103-1111. http://www.scopus.com/inward/record.url?eid=2-s2.0-1942440947&partnerID=MN8TOARS.
  107. Noronha, M.; Lima, J.C.; Bastos, M.; Santos, H.; Maçanita, A.L.. "Unfolding of ubiquitin studied by picosecond time-resolved fluorescence of the tyrosine residue.". Biophysical journal 87 4 (2004): 2609-2620. http://www.scopus.com/inward/record.url?eid=2-s2.0-16644364375&partnerID=MN8TOARS.
    10.1529/biophysj.104.046466
  108. Borges, N.; Marugg, J.D.; Empadinhas, N.; Da Costa, M.S.; Santos, H.. "Specialized Roles of the Two Pathways for the Synthesis of Mannosylglycerate in Osmoadaptation and Thermoadaptation of Rhodothermus marinus". Journal of Biological Chemistry 279 11 (2004): 9892-9898. http://www.scopus.com/inward/record.url?eid=2-s2.0-1642279326&partnerID=MN8TOARS.
    10.1074/jbc.M312186200
  109. Empadinhas, N.; Albuquerque, L.; Costa, J.; Zinder, S.H.; Santos, M.A.S.; Santos, H.; Da Costa, M.S.. "A gene from the mesophilic bacterium Dehalococcoides ethenogenes encodes a novel mannosylglycerate synthase". Journal of Bacteriology 186 13 (2004): 4075-4084. http://www.scopus.com/inward/record.url?eid=2-s2.0-3042523198&partnerID=MN8TOARS.
    10.1128/JB.186.13.4075-4084.2004
  110. Faria, T.Q.; Lima, J.C.; Bastos, M.; Maçanita, A.L.; Santos, H.. "Protein stabilization by osmolytes from hyperthermophiles: Effect of mannosylglycerate on the thermal unfolding of recombinant nuclease a from Staphylococcus aureus studied by picosecond time-resolved fluorescence and calorimetry". Journal of Biological Chemistry 279 47 (2004): 48680-48691. http://www.scopus.com/inward/record.url?eid=2-s2.0-10344236053&partnerID=MN8TOARS.
    10.1074/jbc.M408806200
  111. Sampaio, M.-M.; Santos, H.; Boos, W.. "Synthesis of GDP-mannose and mannosylglycerate from labeled mannose by genetically engineered Escherichia coli without loss of specific isotopic enrichment". Applied and Environmental Microbiology 69 1 (2003): 233-240. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037229829&partnerID=MN8TOARS.
    10.1128/AEM.69.1.233-240.2003
  112. Lamosa, P.; Turner, D.L.; Ventura, R.; Maycock, C.; Santos, H.. "Protein stabilization by compatible solutes: Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR". European Journal of Biochemistry 270 23 (2003): 4606-4614. http://www.scopus.com/inward/record.url?eid=2-s2.0-0344393574&partnerID=MN8TOARS.
    10.1046/j.1432-1033.2003.03861.x
  113. Faria, T.Q.; Knapp, S.; Ladenstein, R.; Maçanita, A.L.; Santos, H.. "Protein stabilisation by compatible solutes: Effect of mannosylglycerate on unfolding thermodynamics and activity of ribonuclease A". ChemBioChem 4 8 (2003): 734-741. http://www.scopus.com/inward/record.url?eid=2-s2.0-0042970474&partnerID=MN8TOARS.
    10.1002/cbic.200300574
  114. Anderson, R.M.; Latorre-Esteves, M.; Neves, A.R.; Lavu, S.; Medvedik, O.; Taylor, C.; Howitz, K.T.; Santos, H.; Sinclair, D.A.. "Yeast Life-Span Extension by Calorie Restriction Is Independent of NAD Fluctuation". Science 302 5653 (2003): 2124-2126. http://www.scopus.com/inward/record.url?eid=2-s2.0-0345731468&partnerID=MN8TOARS.
    10.1126/science.1088697
  115. Gonçalves, L.G.; Huber, R.; Da Costa, M.S.; Santos, H.. "A variant of the hyperthermophile Archaeoglobus fulgidus adapted to grow at high salinity". FEMS Microbiology Letters 218 2 (2003): 239-244. http://www.scopus.com/inward/record.url?eid=2-s2.0-1642346782&partnerID=MN8TOARS.
    10.1016/S0378-1097(02)01146-1
  116. Fareleira, P.; Santos, B.S.; António, C.; Moradas-Ferreira, P.; LeGall, J.; Xavier, A.V.; Santos, H.. "Response of a strict anaerobe to oxygen: Survival strategies in Desulfovibrio gigas". Microbiology 149 6 (2003): 1513-1522. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038480736&partnerID=MN8TOARS.
  117. Lemos, P.C.; Serafim, L.S.; Santos, H.; Reis, M.A.. "Production of polyhydroxyalkanoates by a mixed culture in a sequencing batch reactor: the use of propionate as carbon source.". Communications in agricultural and applied biological sciences 68 2 Pt A (2003): 109-114. http://www.scopus.com/inward/record.url?eid=2-s2.0-4043101032&partnerID=MN8TOARS.
  118. Antunes, A.; Eder, W.; Fareleira, P.; Santos, H.; Huber, R.. "Salinisphaera shabanensis gen. nov., sp. nov., a novel, moderately halophilic bacterium from the brine-seawater interface of the Shaban Deep, Red Sea". Extremophiles 7 1 (2003): 29-34. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037711316&partnerID=MN8TOARS.
    10.1007/s00792-002-0292-5
  119. Johnsen, U.; Selig, M.; Xavier, K.B.; Santos, H.; Schönheit, P.. "Different glycolytic pathways for glucose and fructose in the halophilic archaeon Halococcus saccharolyticus (Archives of Microbiology (2001) 175 (52-61))". Archives of Microbiology 180 6 (2003): http://www.scopus.com/inward/record.url?eid=2-s2.0-0346364905&partnerID=MN8TOARS.
    10.1007/s00203-003-0613-9
  120. Enguita, F.J.; Rodrigues, L.; Archer, M.; Sieker, L.; Rodrigues, A.; Pohl, E.; Turner, D.L.; Santos, H.; Carrondo, M.A.. "Crystallization and preliminary X-ray characterization of cytochrome c'' from the obligate methylotroph Methylophilus methylotrophus". Acta Crystallographica - Section D Biological Crystallography 59 3 (2003): 580-583. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037348494&partnerID=MN8TOARS.
    10.1107/S0907444903001045
  121. Pflüger, K.; Baumann, S.; Gottschalk, G.; Lin, W.; Santos, H.; Müller, V.. "Lysine-2,3-Aminomutase and ß-Lysine Acetyltransferase Genes of Methanogenic Archaea Are Salt Induced and Are Essential for the Biosynthesis of N-Acetyl-ß-Lysine and Growth at High Salinity". Applied and Environmental Microbiology 69 10 (2003): 6047-6055. http://www.scopus.com/inward/record.url?eid=2-s2.0-10744232046&partnerID=MN8TOARS.
    10.1128/AEM.69.10.6047-6055.2003
  122. Empadinhas, N.; Albuquerque, L.; Henne, A.; Santos, H.; Da Costa, M.S.. "The bacterium Thermus thermophilus, like hyperthermophilic archaea, uses a two-step pathway for the synthesis of mannosylglycerate". Applied and Environmental Microbiology 69 6 (2003): 3272-3279. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038655499&partnerID=MN8TOARS.
    10.1128/AEM.69.6.3272-3279.2003
  123. Lemos, P.C.; Serafim, L.S.; Santos, M.M.; Reis, M.A.M.; Santos, H.. "Metabolic Pathway for Propionate Utilization by Phosphorus-Accumulating Organisms in Activated Sludge: 13C Labeling and In Vivo Nuclear Magnetic Resonance". Applied and Environmental Microbiology 69 1 (2003): 241-251. http://www.scopus.com/inward/record.url?eid=2-s2.0-0348209610&partnerID=MN8TOARS.
    10.1128/AEM.69.1.241-251.2003
  124. Borges, N.; Ramos, A.; Raven, N.D.H.; Sharp, R.J.; Santos, H.. "Comparative study of the thermostabilizing properties of mannosylglycerate and other compatible solutes on model enzymes". Extremophiles 6 3 (2002): 209-216. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036599177&partnerID=MN8TOARS.
    10.1007/s007920100236
  125. Neves, A.R.; Ventura, R.; Mansour, N.; Shearman, C.; Gasson, M.J.; Maycock, C.; Ramos, A.; Santos, H.. "Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD+ and NADH pools determined in vivo by 13C NMR". Journal of Biological Chemistry 277 31 (2002): 28088-28098. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037008786&partnerID=MN8TOARS.
    10.1074/jbc.M202573200
  126. Neves, A.R.; Ramos, A.; Costa, H.; Van Swam, I.I.; Hugenholtz, J.; Kleerebezem, M.; De Vos, W.; Santos, H.. "Effect of different NADH oxidase levels on glucose metabolism by Lactococcus lactis: Kinetics of intracellular metabolite pools determined by in vivo nuclear magnetic resonance". Applied and Environmental Microbiology 68 12 (2002): 6332-6342. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036952309&partnerID=MN8TOARS.
    10.1128/AEM.68.12.6332-6342.2002
  127. Serafim, L.S.; Lemos, P.C.; Levantesi, C.; Tandoi, V.; Santos, H.; Reis, M.A.M.. "Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms". Journal of Microbiological Methods 51 1 (2002): 1-18. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036277920&partnerID=MN8TOARS.
    10.1016/S0167-7012(02)00056-8
  128. Santos, H.; Da Costa, M.S.. "Compatible solutes of organisms that live in hot saline environments". Environmental Microbiology 4 9 (2002): 501-509. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036742110&partnerID=MN8TOARS.
    10.1046/j.1462-2920.2002.00335.x
  129. Neves, C.; Santos, H.; Vilas-Boas, L.; Amâncio, S.. "Involvement of free and conjugated polyamines and free amino acids in the adventitious rooting of micropropagated cork oak and grapevine shoots". Plant Physiology and Biochemistry 40 12 (2002): 1071-1080. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036918040&partnerID=MN8TOARS.
    10.1016/S0981-9428(02)01472-9
  130. Hugenholtz, J.; Hunik, J.; Santos, H.; Smid, E.. "Nutraceutical production by propionibacteria". Lait 82 1 (2002): 103-112. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035994477&partnerID=MN8TOARS.
  131. Ramos, A.; Neves, A.R.; Santos, H.. "Metabolism and lactic acid bacteria studied by nuclear magnetic resonance". Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 82 1-4 (2002): 249-261. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036693728&partnerID=MN8TOARS.
    10.1023/A:1020664422633
  132. Antunes, A.; Rainey, F.A.; Nobre, M.F.; Schumann, P.; Ferreira, A.M.; Ramos, A.; Santos, H.; da Costa, M.S.. "Leuconostoc ficulneum sp. nov., a novel lactic acid bacterium isolated from a ripe fig, and reclassification of Lactobacillus fructosus as Leuconostoc fructosum comb. nov.". International Journal of Systematic and Evolutionary Microbiology 52 2 (2002): 647-655. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036206183&partnerID=MN8TOARS.
    10.1099/00207713-52-2-647
  133. Neves, A.R.; Ramos, A.; Shearman, C.; Gasson, M.J.; Santos, H.. "Catabolism of mannitol in Lactococcus lactis MG1363 and a mutant defective in lactate dehydrogenase". Microbiology 148 11 (2002): 3467-3476. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036854436&partnerID=MN8TOARS.
    10.1099/00221287-148-11-3467
  134. Brennan, L.; Turner, D.L.; Fareleira, P.; Santos, H.. "Solution structure of Methylophilus methylotrophus cytochrome c'': Insights into the structural basis of haem-ligand detachment". Journal of Molecular Biology 308 2 (2001): 353-365. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035957884&partnerID=MN8TOARS.
    10.1006/jmbi.2001.4600
  135. Empadinhas, N.; Marugg, J.D.; Borges, N.; Santos, H.; Da Costa, M.S.. "Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii: Biochemical and genetic characterization of key enzymes". Journal of Biological Chemistry 276 47 (2001): 43580-43588. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035941355&partnerID=MN8TOARS.
    10.1074/jbc.M108054200
  136. Ramos, A.; Boels, I.C.; De Vos, W.M.; Santos, H.. "Relationship between glycolysis and exopolysaccharide biosynthesis in Lactococcus lactis". Applied and Environmental Microbiology 67 1 (2001): 33-41. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035167670&partnerID=MN8TOARS.
    10.1128/AEM.67.1.33-41.2001
  137. Fonseca, L.L.; Alves, P.M.; Carrondo, M.J.T.; Santos, H.. "Effect of ethanol on the metabolism of primary astrocytes studied by13C- and31P-NMR spectroscopy". Journal of Neuroscience Research 66 5 (2001): 803-811. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035577802&partnerID=MN8TOARS.
    10.1002/jnr.10039
  138. Alves, A.M.C.R.; Euverink, G.J.W.; Santos, H.; Dijkhuizen, L.. "Different physiological roles of ATP- and PPi-dependent phosphofructokinase isoenzymes in the methylotrophic actinomycete Amycolatopsis methanolica". Journal of Bacteriology 183 24 (2001): 7231-7240. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035212682&partnerID=MN8TOARS.
    10.1128/JB.183.24.7231-7240.2001
  139. Amâncio, S.; Santos, H.; Champigny, M.-L.. "Sucrose synthesis in nitrate fed detached maize and wheat leaves as analyzed by 3C-NMR". Phyton - Annales Rei Botanicae 40 3 (2001): 5-14. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035978221&partnerID=MN8TOARS.
  140. Johnsen, U.; Selig, M.; Xavier, K.B.; Santos, H.; Schönheit, P.. "Different glycolytic pathways for glucose and fructose in the halophilic archaeon Halococcus saccharolyticus". Archives of Microbiology 175 1 (2001): 52-61. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035106495&partnerID=MN8TOARS.
    10.1007/s002030000237
  141. Lamosa, P.; Brennan, L.; Vis, H.; Turner, D.L.; Santos, H.. "NMR structure of Desulfovibrio gigas rubredoxin: A model for studying protein stabilization by compatible solutes". Extremophiles 5 5 (2001): 303-311. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035490249&partnerID=MN8TOARS.
    10.1007/s007920100206
  142. Price, N.J.; Brennan, L.; Faria, T.Q.; Vijgenboom, E.; Canters, G.W.; Turner, D.L.; Santos, H.. "High yield of Methylophilus methylotrophus cytochrome c'' by coexpression with cytochrome c maturation gene cluster from Escherichia coli". Protein Expression and Purification 20 3 (2000): 444-450. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033673137&partnerID=MN8TOARS.
    10.1006/prep.2000.1318
  143. Neves, A.R.; Ramos, A.; Shearman, C.; Gasson, M.J.; Almeida, J.S.; Santos, H.. "Metabolic characterization of Lactococcus lactis deficient in lactate dehydrogenase using in vivo 13C-NMR". European Journal of Biochemistry 267 12 (2000): 3859-3868. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033946783&partnerID=MN8TOARS.
    10.1046/j.1432-1327.2000.01424.x
  144. Alves, P.M.; Nunes, R.; Zhang, C.; Maycock, C.D.; Sonnewald, U.; Carrondo, M.J.T.; Santos, H.. "Metabolism of 3-13C Malate in primary cultures of mouse astrocytes". Developmental Neuroscience 22 5-6 (2000): 456-462. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033625879&partnerID=MN8TOARS.
  145. Indiani, C.; De Sanctis, G.; Neri, F.; Santos, H.; Smulevich, G.; Coletta, M.. "Effect of pH on axial ligand coordination of cytochrome c'' from Methylophilus methylotrophus and horse heart cytochrome c". Biochemistry 39 28 (2000): 8234-8242. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034682558&partnerID=MN8TOARS.
    10.1021/bi000266i
  146. Delpierre, G.; Rider, M.H.; Collard, F.; Stroobant, V.; Vanstapel, F.; Santos, H.; Van Schaftingen, E.. "Identification, cloning, and heterologous expression of a mammalian fructosamine-3-kinase". Diabetes 49 10 (2000): 1627-1634. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033820420&partnerID=MN8TOARS.
  147. Rauter, A.; Ferreira, M.; Borges, C.; Duarte, T.; Piedade, F.; Silva, M.; Santos, H.. "Construction of a branched chain at C-3 of a hexopyranoside. Synthesis of miharamycin sugar moiety analogs". Carbohydrate Research 325 1 (2000): 1-15. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034708525&partnerID=MN8TOARS.
    10.1016/S0008-6215(99)00307-9
  148. Brennan, L.; Turner, D.L.; Messias, A.C.; Teodoro, M.L.; LeGall, J.; Santos, H.; Xavier, A.V.. "Structural basis for the network of functional cooperativities in cytochrome c3 from Desulfovibrio gigas: solution structures of the oxidised and reduced states". Journal of Molecular Biology 298 1 (2000): 61-82. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034696760&partnerID=MN8TOARS.
    10.1006/jmbi.2000.3652
  149. Lamosa, P.; Burke, A.; Peist, R.; Huber, R.; Liu, M.-Y.; Silva, G.; Rodrigues-Pousada, C.; et al. "Thermostabilization of proteins by diglycerol phosphate, a new compatible solute from the hyperthermophile Archaeoglobus fulgidus". Applied and Environmental Microbiology 66 5 (2000): 1974-1979. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034103110&partnerID=MN8TOARS.
    10.1128/AEM.66.5.1974-1979.2000
  150. Alves, P.M.; Fonseca, L.L.; Peixoto, C.C.; Almeida, A.C.; Carrondo, M.J.T.; Santos, H.. "NMR studies on energy metabolism of immobilized primary neurons and astrocytes during hypoxia, ischemia and hypoglycemia". NMR in Biomedicine 13 8 (2000): 438-448. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034493853&partnerID=MN8TOARS.
    10.1002/nbm.665
  151. Xavier, K.B.; Da Costa, M.S.; Santos, H.. "Demonstration of a Novel Glycolytic Pathway in the Hyperthermophilic Archaeon Thermococcus zilligii by13C-Labeling Experiments and Nuclear Magnetic Resonance Analysis". Journal of Bacteriology 182 16 (2000): 4632-4636. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033905714&partnerID=MN8TOARS.
    10.1128/JB.182.16.4632-4636.2000
  152. Cánovas, D.; Borges, N.; Vargas, C.; Ventosa, A.; Nieto, J.J.; Santos, H.. "Role of Ny-Acetyldiaminobutyrate as an Enzyme Stabilizer and an Intermediate in the Biosynthesis of Hydroxyectoine". Applied and Environmental Microbiology 65 9 (1999): 3774-3779. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032586949&partnerID=MN8TOARS.
  153. Santos, M.M.; Lemos, P.C.; Reis, M.A.M.; Santos, H.. "Glucose metabolism and kinetics of phosphorus removal by the fermentative bacterium Microlunatus phosphovorus". Applied and Environmental Microbiology 65 9 (1999): 3920-3928. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032866765&partnerID=MN8TOARS.
  154. Turner, D.L.; Brennan, L.; Meyer, H.E.; Lohaus, C.; Siethoff, C.; Costa, H.S.; Gonzalez, B.; Santos, H.; Suárez, J.E.. "Solution structure of plantaricin C, a novel lantibiotic". European Journal of Biochemistry 264 3 (1999): 833-839. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033568096&partnerID=MN8TOARS.
    10.1046/j.1432-1327.1999.00674.x
  155. Cruz, P.E.; Martins, P.C.; Alves, P.M.; Peixoto, C.C.; Santos, H.; Moreira, J.L.; Carrondo, M.J.T.. "Proteolytic activity in infected and noninfected insect cells: Degradation of HIV-1 Pr55gag particles". Biotechnology and Bioengineering 65 2 (1999): 133-143. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033589370&partnerID=MN8TOARS.
    10.1002/(SICI)1097-0290(19991020)65:2<133::AID-BIT2>3.0.CO;2-X
  156. Klarskov, K.; Leys, D.; Backers, K.; Costa, H.S.; Santos, H.; Guisez, Y.; Van Beeumen, J.J.. "Cytochrome c'' from the obligate methylotroph Methylophilus methylotrophus, an unexpected homolog of sphaeroides heme protein from the phototroph Rhodobacter sphaeroides". Biochimica et Biophysica Acta - Bioenergetics 1412 1 (1999): 47-55. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032915232&partnerID=MN8TOARS.
    10.1016/S0005-2728(99)00050-X
  157. Price, N.J.; Vijgenboom, E.; Ribeiro, G.; Costa, J.V.; Canters, G.W.; Santos, H.. "Cloning and sequence analysis of the gene encoding Methylophilus methylotrophus cytochrome c'', a unique protein with a perpendicular orientation of the histidinyl ligands". Biochimica et Biophysica Acta - Bioenergetics 1413 1 (1999): 55-61. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032866467&partnerID=MN8TOARS.
    10.1016/S0005-2728(99)00085-7
  158. Silva, Z.; Borges, N.; Martins, L.O.; Wait, R.; Da Costa, M.S.; Santos, H.. "Combined effect of the growth temperature and salinity of the medium on the accumulation of compatible solutes by Rhodothermus marinus and Rhodothermus obamensis". Extremophiles 3 2 (1999): 163-172. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033136552&partnerID=MN8TOARS.
    10.1007/s007920050112
  159. Neves, A.R.; Ramos, A.; Nunes, M.C.; Kleerebezem, M.; Hugenholtz, J.; De Vos, W.M.; Almeida, J.; Santos, H.. "In vivo nuclear magnetic resonance studies of glycolytic kinetics in Lactococcus lactis". Biotechnology and Bioengineering 64 2 (1999): 200-212. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033587788&partnerID=MN8TOARS.
    10.1002/(SICI)1097-0290(19990720)64:2<200::AID-BIT9>3.0.CO;2-K
  160. Martins, A.; Santos, M.; Santos, H.; Pais, M.S.. "A 31P nuclear magnetic resonance study of phosphate levels in roots of ectomycorrhizal and nonmycorrhizal plants of Castanea sativa Mill.". Trees - Structure and Function 13 3 (1999): 168-172. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033005288&partnerID=MN8TOARS.
    10.1007/s004680050201
  161. Martins, L.O.; Empadinhas, N.; Marugg, J.D.; Miguel, C.; Ferreira, C.; Da Costa, M.S.; Santos, H.. "Biosynthesis of mannosylglycerate in the thermophilic bacterium Rhodothermus marinus. Biochemical and genetic characterization of a mannosylglycerate synthase". Journal of Biological Chemistry 274 50 (1999): 35407-35414. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033544948&partnerID=MN8TOARS.
    10.1074/jbc.274.50.35407
  162. Xavier, K.B.; Peist, R.; Kossmann, M.; Boos, W.; Santos, H.. "Maltose metabolism in the hyperthermophilic archaeon Thermococcus litoralis: Purification and characterization of key enzymes". Journal of Bacteriology 181 11 (1999): 3358-3367. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033003021&partnerID=MN8TOARS.
  163. Hols, P.; Ramos, A.; Hugenholtz, J.; Delcour, J.; De Vos, W.M.; Santos, H.; Kleerebezem, M.. "Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: A rescue pathway for maintaining redox balance". Journal of Bacteriology 181 17 (1999): 5521-5526. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032879297&partnerID=MN8TOARS.
  164. Messias, A.C.; Kastrau, D.H.W.; Costa, H.S.; Legall, J.; Turner, D.L.; Santos, H.; Xavier, A.V.. "Solution structure of Desulfovibrio vulgaris (Hildenborough) ferrocytochrome c3: Structural basis for functional cooperativity". Journal of Molecular Biology 281 4 (1998): 719-739. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032575765&partnerID=MN8TOARS.
    10.1006/jmbi.1998.1974
  165. Houbiers, C.; Lima, J.C.; Maçanita, A.L.; Santos, H.. "Color stabilization of malvidin 3-glucoside: Self-aggregation of the flavylium cation and copigmentation with the Z-chalcone form". Journal of Physical Chemistry B 102 18 (1998): 3578-3585. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001685609&partnerID=MN8TOARS.
  166. Lamosa, P.; Martins, L.O.; Da Costa, M.S.; Santos, H.. "Effects of temperature, salinity, and medium composition on compatible solute accumulation by Thermococcus spp.". Applied and Environmental Microbiology 64 10 (1998): 3591-3598. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031662628&partnerID=MN8TOARS.
  167. da Costa, M.S.; Santos, H.; Galinski, E.A.. "An overview of the role and diversity of compatible solutes in Bacteria and Archaea.". Advances in biochemical engineering/biotechnology 61 (1998): 117-153. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031613817&partnerID=MN8TOARS.
  168. Pina, F.; Melo, M.J.; Santos, H.; Lima, J.C.; Abreu, I.; Ballardini, R.; Maestri, M.. "Excited state proton transfer in synthetic flavylium salts: 4-methyl-7-hydroxyflavylium and 4',7-dihydroxyflavylium Example of a four-level molecular device to invert the population of the excited state". New Journal of Chemistry 22 10 (1998): 1093-1098. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000661579&partnerID=MN8TOARS.
  169. Horlacher, R.; Xavier, K.B.; Santos, H.; Diruggiero, J.; Kossmann, M.; Boos, W.. "Archaeal binding protein-dependent ABC transporter: Molecular and biochemical analysis of the trehalose/maltose transport system of the hyperthermophilic archaeon Thermococcus litoralis". Journal of Bacteriology 180 3 (1998): 680-689. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031888811&partnerID=MN8TOARS.
  170. Carmelo, V.; Santos, H.; Sá-Correia, I.. "Effect of extracellular acidification on the activity of plasma membrane ATPase and on the cytosolic and vacuolar pH of Saccharomyces cerevisiae". Biochimica et Biophysica Acta - Biomembranes 1325 1 (1997): 63-70. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031551022&partnerID=MN8TOARS.
    10.1016/S0005-2736(96)00245-3
  171. Coletta, M.; Costa, H.; De Sanctis, G.; Neri, F.; Smulevich, G.; Turner, D.L.; Santos, H.. "pH dependence of structural and functional properties of oxidized cytochrome c'' from Methylophilus methylotrophus". Journal of Biological Chemistry 272 40 (1997): 24800-24804. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030770420&partnerID=MN8TOARS.
    10.1074/jbc.272.40.24800
  172. Miranda, M.; Ramos, A.; Veiga-da-Cunha, M.; Loureiro-Dias, M.C.; Santos, H.. "Biochemical basis for glucose-induced inhibition of malolactic fermentation in Leuconostoc oenos". Journal of Bacteriology 179 17 (1997): 5347-5354. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030923597&partnerID=MN8TOARS.
    10.1128/jb.179.17.5347-5354.1997
  173. Fareleira, P.; Legall, J.; Xavier, A.V.; Santos, H.. "Pathways for utilization of carbon reserves in Desulfovibrio gigas under fermentative and respiratory conditions". Journal of Bacteriology 179 12 (1997): 3972-3980. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030924390&partnerID=MN8TOARS.
    10.1128/jb.179.12.3972-3980.1997
  174. Selig, M.; Xavier, K.B.; Santos, H.; Schönheit, P.. "Comparative analysis of Embden-Meyerhof and Entner-Doudoroff glycolytic pathways in hyperthermophilic archaea and the bacterium Thermotoga". Archives of Microbiology 167 4 (1997): 217-232. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031002263&partnerID=MN8TOARS.
    10.1007/BF03356097
  175. Ramos, A.; Raven, N.D.H.; Sharp, R.J.; Bartolucci, S.; Rossi, M.; Cannio, R.; Lebbink, J.; et al. "Stabilization of enzymes against thermal stress and freeze-drying by mannosylglycerate". Applied and Environmental Microbiology 63 10 (1997): 4020-4025. http://www.scopus.com/inward/record.url?eid=2-s2.0-16744366799&partnerID=MN8TOARS.
  176. Amancio, S.; Clarkson, D.T.; Diogo, E.; Lewis, M.; Santos, H.. "Assimilation of nitrate and ammonium by sulphur deficient Zea mays cells". Plant Physiology and Biochemistry 35 1 (1997): 41-48. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030893715&partnerID=MN8TOARS.
  177. Martins, L.O.; Huber, R.; Huber, H.; Stetter, K.O.; Da Costa, M.S.; Santos, H.. "Organic solutes in hyperthermophilic Archaea". Applied and Environmental Microbiology 63 3 (1997): 896-902. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031020672&partnerID=MN8TOARS.
  178. Sijbesma, Wilbert F. H.; Almeida, Jonas S.; Reis, Maria A. M.; Santos, Helena. "Uncoupling effect of nitrite during denitrification byPseudomonas fluorescens: An in vivo31P-NMR study". Biotechnology and Bioengineering 52 1 (1996): 176-182. http://dx.doi.org/10.1002/(sici)1097-0290(19961005)52:1<176::aid-bit18>3.0.co;2-m.
    10.1002/(sici)1097-0290(19961005)52:1<176::aid-bit18>3.0.co;2-m
  179. Pereira, Helena; Lemos, Paulo C.; Reis, Maria A.M.; Crespo, João P.S.G.; Carrondo, Manuel J.T.; Santos, Helena. "Model for carbon metabolism in biological phosphorus removal processes based on in vivo13C-NMR labelling experiments". Water Research 30 9 (1996): 2128-2138. http://dx.doi.org/10.1016/0043-1354(96)00035-8.
    10.1016/0043-1354(96)00035-8
  180. Costa, H.S.; Santos, H.; Turner, D.L.. "Ligand orientation and haem electronic structure in ferricytochrome c'' from Methylophilus methylotrophus studied by 13C NMR". European Biophysics Journal 25 1 (1996): 19-24. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029907073&partnerID=MN8TOARS.
    10.1007/s002490050011
  181. Hensgens, C.M.H.; Santos, H.; Zhang, C.; Kruizinga, W.H.; Hansen, T.A.. "Electron-dense granules in Desulfovibrio gigas do not consist of inorganic triphosphate but of a glucose pentakis(diphosphate)". European Journal of Biochemistry 242 2 (1996): 327-331. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029828582&partnerID=MN8TOARS.
  182. Ramos, A.; Santos, H.. "Citrate and sugar cofermentation in Leuconostoc oenos, a 13C nuclear magnetic resonance study". Applied and Environmental Microbiology 62 7 (1996): 2577-2585. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029949630&partnerID=MN8TOARS.
  183. Xavier, K.B.; Martins, L.O.; Peist, R.; Kossmann, M.; Boos, W.; Santos, H.. "High-affinity maltose/trehalose transport system in the hyperthermophilic archaeon Thermococcus litoralis". Journal of Bacteriology 178 16 (1996): 4773-4777. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029779716&partnerID=MN8TOARS.
    10.1128/jb.178.16.4773-4777.1996
  184. Alves, P.M.; Flögel, U.; Brand, A.; Leihfritz, D.; Carrondo, M.J.T.; Santos, H.; Sonnewald, U.. "Immobilization of primary astrocytes and neurons for on-line monitoring of biochemical processes by nmr". Developmental Neuroscience 18 5-6 (1996): 478-483. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029850062&partnerID=MN8TOARS.
    10.1159/000111443
  185. Martins, L.O.; Carreto, L.S.; Da Costa, M.S.; Santos, H.. "New compatible solutes related to di-myo-inositol-phosphate in members of the order Thermotogales". Journal of Bacteriology 178 19 (1996): 5644-5651. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029838122&partnerID=MN8TOARS.
    10.1128/jb.178.19.5644-5651.1996
  186. Chen, Liang; LeGall, Jean; Fareleira, Paula; Santos, Helena; Xavier, António V.. "Malate Metabolism by Desulfovibrio gigas and its Link to Sulfate and Fumarate Reduction: Purification of the Malic Enzyme and Detection of NAD(P)+ Transhydrogenase Activity". Anaerobe 1 4 (1995): 227-235. http://dx.doi.org/10.1006/anae.1995.1022.
    10.1006/anae.1995.1022
  187. Ramos, A.; Lolkema, J.S.; Konings, W.N.; Santos, H.. "Enzyme basis for pH regulation of citrate and pyruvate metabolism by Leuconostoc oenos". Applied and Environmental Microbiology 61 4 (1995): 1303-1310. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028949653&partnerID=MN8TOARS.
  188. Nunes, O.C.; Manaia, C.M.; Da Costa, M.S.; Santos, H.. "Compatible solutes in the thermophilic bacteria Rhodothermus marinus and 'Thermus thermophilus'". Applied and Environmental Microbiology 61 6 (1995): 2351-2357. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029031690&partnerID=MN8TOARS.
  189. Martins, L.O.; Santos, H.. "Accumulation of mannosylglycerate and Di-myo-inositol-phosphate by Pyrococcus furiosus in response to salinity and temperature". Applied and Environmental Microbiology 61 9 (1995): 3299-3303. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028981456&partnerID=MN8TOARS.
  190. Xavier, K.B.; Kossmann, M.; Santos, H.; Boos, W.. "Kinetic analysis by in vivo 31P nuclear magnetic resonance of internal P(i) during the uptake of sn-glycerol-3-phosphate by the pho regulon- dependent Ugp system and the glp regulon-dependent GlpT system". Journal of Bacteriology 177 3 (1995): 699-704. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028952583&partnerID=MN8TOARS.
  191. COSTA, Helena S.; SANTOS, Helena; TURNER, David L.. "An unusual conformation of the methionine haem ligand in cytochrome cL established by two-dimensional 1H-NMR". European Journal of Biochemistry 223 3 (1994): 783-789. http://dx.doi.org/10.1111/j.1432-1033.1994.tb19053.x.
    10.1111/j.1432-1033.1994.tb19053.x
  192. Schäfer, Thomas; Xavier, Karina B.; Santos, Helena; Schönheit, Peter. "Glucose fermentation to acetate and alanine in resting cell suspensions of Pyrococcus furiosus: Proposal of a novel glycolytic pathway based on 13C labelling data and enzyme activities". FEMS Microbiology Letters 121 1 (1994): 107-114. http://dx.doi.org/10.1111/j.1574-6968.1994.tb07083.x.
    10.1111/j.1574-6968.1994.tb07083.x
  193. Figueiredo, P.; Lima, J.C.; Santos, H.; Maçanita, A.L.; Pina, F.; Wigand, M.-C.; Brouillard, R.. "Photochromism of the Synthetic 4',7-Dihydroxyflavylium Chloride". Journal of the American Chemical Society 116 4 (1994): 1249-1254. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000660025&partnerID=MN8TOARS.
    10.1021/ja00083a011
  194. Ramos, Ana; Jordan, Kieran N.; Cogan, Timothy M.; Santos, Helena. "13C Nuclear Magnetic Resonance Studies of Citrate and Glucose Cometabolism by Lactococcus lactis". Applied and Environmental Microbiology 60 6 (1994): 1739-1748. http://dx.doi.org/10.1128/aem.60.6.1739-1748.1994.
    10.1128/aem.60.6.1739-1748.1994
  195. Ramos, A.; Poolman, B.; Santos, H.; Lolkema, J.S.; Konings, W.N.. "Uniport of anionic citrate and proton consumption in citrate metabolism generates a proton motive force in Leuconostoc oenos". Journal of Bacteriology 176 16 (1994): 4899-4905. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028128858&partnerID=MN8TOARS.
    10.1128/jb.176.16.4899-4905.1994
  196. Santos, Helena; Turner, David L.. "Determination of the methionine haem ligand conformation in cytochromecH fromMethylophilus methylotrophus by two-dimensional 1H NMR". Magnetic Resonance in Chemistry 31 13 (1993): S90-S95. http://dx.doi.org/10.1002/mrc.1260311318.
    10.1002/mrc.1260311318
  197. Amancio, Sara; Diogo, Eugenio; Santos, Helena. "Effects of the source of inorganic nitrogen on C and N interaction in maize callus tissue: phosphoenolpyruvate carboxylase activity, cytosolic pH and 15N amino acids". Physiologia Plantarum 89 3 (1993): 618-625. http://dx.doi.org/10.1034/j.1399-3054.1993.890332.x.
    10.1034/j.1399-3054.1993.890332.x
  198. COSTA, Helena S.; SANTOS, Helena; TURNER, David L.. "Characterization of the haem environment in Methylophilus methylotrophus ferricytochrome c" by 1H-NMR". European Journal of Biochemistry 215 3 (1993): 817-824. http://dx.doi.org/10.1111/j.1432-1033.1993.tb18097.x.
    10.1111/j.1432-1033.1993.tb18097.x
  199. Santos, H.; Fareleira, P.; Xavier, A.V.; Chen, L.; Liu, M.-Y.; Legall, J.. "Aerobic Metabolism of Carbon Reserves by the “Obligate Anaerobe” Desulfovibrio gigas". Biochemical and Biophysical Research Communications 195 2 (1993): 551-557. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027249337&partnerID=MN8TOARS.
    10.1006/bbrc.1993.2081
  200. Teixeira, M.; Campos, A.P.; Aguiar, A.P.; Costa, H.S.; Santos, H.; Turner, D.L.; Xavier, A.V.. "Pitfalls in assigning heme axial coordination by EPR. c-Type cytochromes with atypical Met-His ligation". FEBS Letters 317 3 (1993): 233-236. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027407150&partnerID=MN8TOARS.
    10.1016/0014-5793(93)81282-5
  201. Taipa, M.A.; Cabral, J.M.S.; Santos, H.. "Comparison of glucose fermentation by suspended and gel-entrapped yeast cells: An in vivo nuclear magnetic resonance study". Biotechnology and Bioengineering 41 6 (1993): 647-653. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027572086&partnerID=MN8TOARS.
    10.1002/bit.260410607
  202. Santos, H.; Turner, D.L.; Lima, J.C.; Figueiredo, P.; Pina, F.S.; Maçanita, A.L.. "Elucidation of the multiple equilibria of malvin in aqueous solution by One- and two-dimensional NMR". Phytochemistry 33 5 (1993): 1227-1232. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001303721&partnerID=MN8TOARS.
    10.1016/0031-9422(93)85054-U
  203. CHEN, L.; LIU, M.-Y.; LEGALL, J.; FARELEIRA, P.; SANTOS, H.; XAVIER, A.V.. "Purification and characterization of an NADH–rubredoxin oxidoreductase involved in the utilization of oxygen by Desulfovibrio gigas". European Journal of Biochemistry 216 2 (1993): 443-448. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027197351&partnerID=MN8TOARS.
    10.1111/j.1432-1033.1993.tb18162.x
  204. Chen, L.; Liu, M.Y.; LeGall, J.; Fareleira, P.; Santos, H.; Xavier, A.V.. "Rubredoxin Oxidase, a New Flavo-Hemo-Protein, Is the Site of Oxygen Reduction to Water by the "Strict Anaerobe" Desulfovibrio gigas". Biochemical and Biophysical Research Communications 193 1 (1993): 100-105. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027263895&partnerID=MN8TOARS.
    10.1006/bbrc.1993.1595
  205. Veiga-Da-Cunha, M.; Santos, H.; Van Schaftingen, E.. "Pathway and regulation of erythritol formation in Leuconostoc oenos". Journal of Bacteriology 175 13 (1993): 3941-3948. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027209897&partnerID=MN8TOARS.
    10.1128/jb.175.13.3941-3948.1993
  206. Salgueiro, Carlos A.; Turner, David L.; Santos, Helena; LeGall, Jean; Xavier, António V.. "Assignment of the redox potentials to the four haems in Desulfovibrio vulgaris cytochrome c 3 by 2D-NMR". FEBS Letters 314 2 (1992): 155-158. http://dx.doi.org/10.1016/0014-5793(92)80963-h.
    10.1016/0014-5793(92)80963-h
  207. COSTA, Helena S.; SANTOS, Helena; TURNER, David L.; XAVIER, Antonio V.. "Involvement of a labile axial histidine in coupling electron and proton transfer in Methylophilus methylotrophus cytochrome c"". European Journal of Biochemistry 208 2 (1992): 427-433. http://dx.doi.org/10.1111/j.1432-1033.1992.tb17204.x.
    10.1111/j.1432-1033.1992.tb17204.x
  208. SANTOS, H.; TURNER, D.L.. "13C and proton NMR studies of horse cytochrome c Systematic assignment of methyl and methine resonances in both oxidation states". European Journal of Biochemistry 206 3 (1992): 721-728. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026610916&partnerID=MN8TOARS.
    10.1111/j.1432-1033.1992.tb16978.x
  209. Amancio, S.; Santos, H.. "Nitrate and Ammonium Assimilation by Roots of Maize (Zea maysL.) Seedlings as Investigated byIn Vivo15N-NMR". Journal of Experimental Botany 43 5 (1992): 633-639. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000778509&partnerID=MN8TOARS.
    10.1093/jxb/43.5.633
  210. Oliveira, A.P.; Marques, J.F.; Santos, H.. "Nodulation in clover roots: Correlation with vacuolar pH". FEMS Microbiology Letters 96 2-3 (1992): 119-123. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026669690&partnerID=MN8TOARS.
    10.1016/0378-1097(92)90390-A
  211. Turner, D.L.; Santos, H.; Fareleira, P.; Pacheco, I.; LeGall, J.; Xavier, A.V.. "Structure determination of a novel cyclic phosphocompound isolated from Desulfovibrio desulfuricans". Biochemical Journal 285 2 (1992): 387-390. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026655348&partnerID=MN8TOARS.
  212. Veiga-Da-Cunha, Maria; Firme, Paula; Romão, M. Vitória San; Santos, Helena. "Application of 13C Nuclear Magnetic Resonance To Elucidate the Unexpected Biosynthesis of Erythritol by Leuconostoc oenos". Applied and Environmental Microbiology 58 7 (1992): 2271-2279. http://dx.doi.org/10.1128/aem.58.7.2271-2279.1992.
    10.1128/aem.58.7.2271-2279.1992
  213. SANTOS, Helena; FARELEIRA, Paula; PEDREGAL, Carmen; LeGALL, Jean; XAVIER, Antonio V.. "In vivo 31P-NMR studies of Desulfovibrio species. Detection of a novel phosphorus-containing compound". European Journal of Biochemistry 201 1 (1991): 283-287. http://dx.doi.org/10.1111/j.1432-1033.1991.tb16285.x.
    10.1111/j.1432-1033.1991.tb16285.x
  214. Melo, E.C.C.; Costa, S.M.B.; Maçanta, A.L.; Santos, H.. "The use of the n-(9-anthroyloxy) stearic acid to probe the water content of sodium dodecyl sulfate, dodecyltimethylammonium chloride, and triton X-100 micelles". Journal of Colloid And Interface Science 141 2 (1991): 439-453. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026104016&partnerID=MN8TOARS.
    10.1016/0021-9797(91)90341-5
  215. Berry, M J; George, S J; Thomson, A J; Santos, H; Turner, D L. "Cytochrome c11 isolated from Methylophilus methylotrophus. An example of bis-histidine-co-ordinated Fe3+ haem, with near-perpendicular orientation of the ligands". Biochemical Journal 270 2 (1990): 413-417. http://dx.doi.org/10.1042/bj2700413.
    10.1042/bj2700413
  216. Loureiro-Dias, Maria C.; Santos, Helena. "Effects of ethanol on Saccharomyces cerevisiae as monitored by in vivo 31P and 13C nuclear magnetic resonance". Archives of Microbiology 153 4 (1990): 384-391. http://dx.doi.org/10.1007/bf00249010.
    10.1007/bf00249010
  217. Santos, H.; Fareleha, P.; LeGall, J.; Xavier, A.V.. "In vivo nuclear magnetic resonance studies of the metabolism of methanol and pyruvate by Methanosarcina barkeri". FEMS Microbiology Letters 87 3-4 (1990): 361-366. http://www.scopus.com/inward/record.url?eid=2-s2.0-27844577291&partnerID=MN8TOARS.
    10.1016/0378-1097(90)90479-A
  218. Santos, H.; Fareleira, P.; LeGall, J.; Xavier, A.V.. "In vivo nuclear magnetic resonance studies of the metabolism of methanol and pyruvate by Methanosarcina barkeri". FEMS Microbiology Reviews 87 3-4 (1990): 361-366. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025605867&partnerID=MN8TOARS.
  219. SANTOS, Helena; FARELEIRA, Paula; TOCI, Rene; LeGALL, Jean; PECK, Harry D.; XAVIER, Antonio V.. "In vivo 31P-and 13C-NMR studies of ATP synthesis and methane formation by Methanosarcina barkeri". European Journal of Biochemistry 180 2 (1989): 421-427. http://dx.doi.org/10.1111/j.1432-1033.1989.tb14663.x.
    10.1111/j.1432-1033.1989.tb14663.x
  220. Loureiro-Dias, Maria C.; Santos, Helena. "Effects of 2-deoxyglucose on Saccharomyces cerevisiaeas observed by in vivo31P-NMR". FEMS Microbiology Letters 57 1 (1989): 25-28. http://dx.doi.org/10.1111/j.1574-6968.1989.tb03214.x.
    10.1111/j.1574-6968.1989.tb03214.x
  221. Maçanita, A.L.; Costa, F.P.; Costa, S.M.B.; Melo, E.C.; Santos, H.. "The 9-anthroate chromophore as a fluorescent probe for water". Journal of Physical Chemistry 93 1 (1989): 336-343. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000525874&partnerID=MN8TOARS.
    10.1021/j100338a066
  222. Santos, H.; Turner, D.L.. "Characterization and NMR studies of a novel cytochrome c isolated from Methylophilus methylotrophus which shows a redox-linked change of spin state". Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 954 C (1988): 277-286. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000292254&partnerID=MN8TOARS.
    10.1016/0167-4838(88)90083-0
  223. Santos, H.; Turner, D.L.. "Proton NMR studies of horse ferricytochrome c Completion of the assignment of the well resolved hyperfine shifted resonances". FEBS Letters 226 1 (1987): 179-185. http://www.scopus.com/inward/record.url?eid=2-s2.0-0023662115&partnerID=MN8TOARS.
    10.1016/0014-5793(87)80575-6
  224. Santos, H.; Turner, D.L.. "Characterization of the improved sensitivity obtained using a flow method for oxygenating and mixing cell suspensions in NMR". Journal of Magnetic Resonance (1969) 68 2 (1986): 345-349. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000600567&partnerID=MN8TOARS.
    10.1016/0022-2364(86)90251-9
  225. Santos, Helena; Turner, David L.. "13 C-NMR studies of horse ferrocytochrome c". FEBS Letters 184 2 (1985): 240-244. http://dx.doi.org/10.1016/0014-5793(85)80614-1.
    10.1016/0014-5793(85)80614-1
  226. Santos, Helena; Turner, David L.. "13 C-NMR studies of horse ferrocytochrome c". FEBS Letters 184 2 (1985): 240-244. http://dx.doi.org/10.1016/0014-5793(85)80614-1.
    10.1016/0014-5793(85)80614-1
  227. Moura, Teresa F.; Macey, Robert I.; Chien, David Y.; Karan, Daniel; Santos, Helena. "Thermodynamics of all-or-none water channel closure in red cells". The Journal of Membrane Biology 81 2 (1984): 105-111. http://dx.doi.org/10.1007/bf01868975.
    10.1007/bf01868975
  228. SANTOS, H.; MOURA, J.J.G.; MOURA, I.; LeGALL, J.; XAVIER, A.V.. "NMR studies of electron transfer mechanisms in a protein with interacting redox centres: Desulfovibrio gigas cytochrome c3". European Journal of Biochemistry 141 2 (1984): 283-296. http://www.scopus.com/inward/record.url?eid=2-s2.0-0021446473&partnerID=MN8TOARS.
    10.1111/j.1432-1033.1984.tb08190.x
  229. Santos, H.; Turner, D.L.; Xavier, A.V.; le Gall, J.. "Two-dimensional NMR studies of electron transfer in cytochrome c3". Journal of Magnetic Resonance (1969) 59 1 (1984): 177-180. http://www.scopus.com/inward/record.url?eid=2-s2.0-0347370769&partnerID=MN8TOARS.
    10.1016/0022-2364(84)90298-1
  230. Santos, H.; Turner, D.L.; Xavier, A.V.. "Four quartets. Application to two-dimensional NMR". Journal of Magnetic Resonance (1969) 58 2 (1984): 344-347. http://www.scopus.com/inward/record.url?eid=2-s2.0-48749141396&partnerID=MN8TOARS.
    10.1016/0022-2364(84)90226-9
  231. Geraldes, C.F.G.C.; Santos, H.. "Solvent effects on the conformation of nucleotides. Part 1. The conformation of 5'-adenosine monophosphate in water-dimethyl sulphoxide using nuclear overhauser effects and lanthanide relaxation probes". Journal of the Chemical Society, Perkin Transactions 2 9 (1983): 1693-1697. http://www.scopus.com/inward/record.url?eid=2-s2.0-37049112565&partnerID=MN8TOARS.
    Published • 10.1039/P29830001693
  232. Santos, H.; Turner, D.L.; Xavier, A.V.. "A difference method for the reduction of "auto" peaks in autocorrelation spectra". Journal of Magnetic Resonance (1969) 55 3 (1983): 463-467. http://www.scopus.com/inward/record.url?eid=2-s2.0-9944230109&partnerID=MN8TOARS.
    10.1016/0022-2364(83)90128-2
  233. Moura, I.; Moura, JosJ.G.; Santos, H.; Xavier, A.V.; Burch, G.; Peck Jr., H.D.; LeGall, J.. "Proteins containing the factor F430 from methanosarcina barkeri and methanobacterium thermoautotrophicum". Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 742 1 (1983): 84-90. http://www.scopus.com/inward/record.url?eid=2-s2.0-0020655696&partnerID=MN8TOARS.
    Published • 10.1016/0167-4838(83)90362-X
  234. Moura, JoséJ.G.; Moura, Isabel; Santos, Helena; Xavier, António V.; Scandellari, Monique; LeGall, Jean. "Isolation of P590 from Methanosarcina barkeri: Evidence for the presence of sulfite reductase activity". Biochemical and Biophysical Research Communications 108 3 (1982): 1002-1009. http://dx.doi.org/10.1016/0006-291x(82)92099-x.
    10.1016/0006-291x(82)92099-x
  235. Wood, J.; Moura, I; Moura, J.; Santos, M.; Xavier, A.; LeGall, J; Scandellari, M. "Role of vitamin B12 in methyl transfer for methane biosynthesis by Methanosarcina barkeri". Science 216 4543 (1982): 303-305. http://dx.doi.org/10.1126/science.7063887.
    10.1126/science.7063887
  236. MOURA, I.; MOURA, J.J.G.; HUYNH, B.-H.; SANTOS, H.; LeGALL, J.; XAVIER, A.V.. "Ferredoxin from Methanosarcina barkeri: Evidence for the Presence of a Three-Iron Center". European Journal of Biochemistry 126 1 (1982): 95-98. http://www.scopus.com/inward/record.url?eid=2-s2.0-0020170092&partnerID=MN8TOARS.
    Published • 10.1111/j.1432-1033.1982.tb06751.x
  237. MOURA, J.J.G.; SANTOS, H.; MOURA, I.; LeGALL, J.; MOORE, G.R.; WILLIAMS, R.J.P.; XAVIER, A.V.. "NMR Redox Studies of Desrulfovibrio vulgaris Cytochrome c3. Electron Transfer Mechanisms". European Journal of Biochemistry 127 1 (1982): 151-155. http://www.scopus.com/inward/record.url?eid=2-s2.0-0020365238&partnerID=MN8TOARS.
    Published • 10.1111/j.1432-1033.1982.tb06849.x
  238. Moura, I.; Moura, J.J.G.; Santos, M.H.; Xavier, A.V.; Le Gall, J.. "Redox studies on rubredoxins from sulphate and sulphur reducing bacteria". FEBS Letters 107 2 (1979): 419-421. http://dx.doi.org/10.1016/0014-5793(79)80421-4.
    10.1016/0014-5793(79)80421-4
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2007 - 2011 Heat Stress Adaptation in Hyperthermophiles: Biosynthesis of Inositol-Containing Compatible Solutes
Supervisor
2005 - 2009 Sugar Metabolism in Lactococcus lactis – Glucose transporters and a novel ¿-phosphoglucomutase
Supervisor
2004 - 2008 Metabolic Engineering of Lactococcus lactis for Mannitol Production. The role of NADH-dependent dehydrogenases in directing carbon fluxes
Supervisor
2004 - 2008 Osmo- and Thermo-Adaptation in Hyperthermophilic Archaea. Identification of compatible solutes, accumulation profiles, and biosynthetic routes in Archaeoglobus spp.
Supervisor
2004 - 2008 Transport, Metabolism, and Physiological Roles of Compatible Solutes of Thermophilic Bacteria: Rhodothermus marinus and Petrotoga miotherma
Supervisor
2003 - 2007 Time-Resolved Fluorescence of Tyrosine Residues: A New Tool to Characterize Protein Conformational Changes
Co-supervisor
2002 - 2006 Metabolism of Primary Astrocytes Studied by NMR: Metabolic Trafficking and Neuroprotection
Supervisor
2001 - 2005 Understanding Protein Stabilization by Solutes from Hyperthermophiles: Effect of Mannosylglycerate on Protein Unfolding Thermodynamics and Enzyme activity
Supervisor
2001 - 2005 Engineering Escherichia coli for the Synthesis of Mannosylglycerate, a Solute Widely Distributed in (Hyper)thermophiles. Transport, Metabolism and Physiological Role
Supervisor
2000 - 2004 The Role of Mannosylglycerate in Thermo- and Osmo-Adaptation of Rhodothermus marinus: Biosynthesis, Regulation and Applications
Supervisor
1998 - 2002 Protein Stabilisation by Diglycerol Phosphate, a Compatible Solute from Hyperthermophiles
Supervisor
1996 - 2001 In Vivo NMR Studies on the Metabolism of Primary Brain Cell Cultures
Supervisor
1996 - 2001 Metabolic Strategies to Reroute Carbon Fluxes in Lactococcus lactis. Kinetics of Intracellular Metabolite Pools using in vivo Nuclear Magnetic Resonance
Supervisor
1995 - 2000 NMR Solution Structure of Cytochromes c3: Insight into the Structural Origins of Functional Cooperativities
Supervisor
1996 - 1999 Carbohydrate Metabolism in Hyperthermophilic Micro-organisms
Supervisor
1994 - 1997 Relação entre o Metabolismo de Fósforo e Carbono em Bactérias Acumuladoras de Polifosfato: Estudos de Ressonância Magnética Nuclear in vivo
Supervisor
1992 - 1995 Estudos de Bioconversão de Energia em Microrganismos por Técnicas não Invasivas
Supervisor
1992 - 1995 Metabolismo de Compostos Carbonados (Ácido Cítrico e Açúcares) Investigado por Ressonância Magnética Nuclear em Bactérias Lácticas.
Supervisor
1992 - 1995 Relação Estrutura/Função em Citocromos de Bactérias Metilotróficas
Supervisor

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2002 - 2002 FEBS ADVANCED COURSE on ADVANCED TECHNOLOGIES TO METABOLIC ENGINEERING IN BIOTECHNOLOGY AND MEDICINE, Oeiras, Portugal (2002/09/07 - 2002/09/13)
1993 - 1993 TWO-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE, Summer Course in collaboration with BRUKER-France, Oeiras, Portugal (1993/09/19 - 1993/09/23)
1992 - 1992 FEBS ADVANCED COURSE on APPLICATIONS OF IN VIVO NMR TECHNIQUES TO PROBE METABOLISM IN YEAST AND OTHER ORGANISMS, Oeiras, Portugal (1992/09/06 - 1992/09/18)
Other

Association member

Society Organization name Role
2009 - Current Academy of Sciences of Lisbon

Conference scientific committee

Conference name Conference host
2010/06/12 - 2010/06/16 8TH INTERNATIONAL CONGRESS ON EXTREMOPHILES, Ponta Delgada, Azores, Portugal
1999/10/03 - 1999/10/06 1st Workshop of the COST Action 624, OPTIMAL MANAGEMENT OF WASTEWATER SYSTEMS, Tomar, Portugal
1999/08/30 - 1999/09/01 TRIS 99, ANNUAL MEETING OF THE FAST REACTIONS IN SOLUTION DISCUSSION GROUP, Universidade Técnica de Lisboa, Instituto Superior Técnico
1997/05/31 - 1997/06/03 1st Meeting of Project “STRATEGIC AND APPLIED RESEARCH ON LACTIC ACID BACTERIA” EU BIOTECH Program, Ericeira, Portugal
1997/05/15 - 1997/05/18 EUROCONFERÊNCIA DA EUROPEAN FEDERATION OF BIOTECHNOLOGY - Microbial Responses to Stress: what’s new and how can it be applied?, Sesimbra, Portugal
1997/05/03 - 1997/05/07 - NATO/ESF ADVANCED RESEARCH WORKSHOP on Biological Electron Transfer Chains: Genetics, Composition and Mode of Action, Tomar, Portugal
1996/06/02 - 1996/06/06 FIRST INTERNATIONAL CONGRESS ON EXTREMOPHILES, Estoril, Portugal
1993/11/07 - 1993/11/09 3rd Meeting on Metabolism and Screening of T Project BRIDGE, BIOTECHNOLOGY OF LACTIC ACID BACTERIA, Funchal, Portugal
1991/04/07 - 1991/04/10 1st Meeting of the T-BRIDGE Project, BIOTECHNOLOGY OF LACTIC ACID BACTERIA, Estoril, Portugal
1990/07/05 - 1990/07/07 1ºEncontro da Associação Portuguesa de Ressonância Magnética em Ciência e Medicina.

Journal scientific committee

Journal title (ISSN) Publisher
2001 - 2011 Extremophiles
2006 - 2009 FEBS Journal
Distinctions

Award

2019 Medal of Science Performance
2019 Prize António Xavier 2019
2019 Prize Nicolau Van Uden 2019
2016 Lifetime Achievement Award
2014 Medalha de Honra Mérito Carreira
2007 Prémio Câmara Pestana 2007
2004 Estímulo à Excelência
1998 Prémio Gulbenkian de Ciência 1998

Title

2019 Full Member of the Portuguese Academy of Sciences
2009 Corresponding Member of the Portuguese Academy of Sciences
Academia das Ciências de Lisboa, Portugal