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Ivo F. Machado is a PhD student in the PhD Programme in Experimental Biology and Biomedicine (PDBEB) by University of Coimbra. He has been conducting research at the University of Coimbra, further enriched by a year as a visiting PhD student at Harvard Medical School. He is the author/co-author of twelve papers in specialized journals.
Identification

Personal identification

Full name
Ivo Manuel Ferreira Machado

Citation names

  • Machado, I.F.
  • Machado, Ivo F.

Author identifiers

Ciência ID
BD1D-EF4F-2F65
ORCID iD
0000-0002-9666-4594

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Cell Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Portuguese (Mother tongue)
Education
Degree Classification
2020/12 - 2025
Ongoing
 PhD Programme in Experimental Biology and Biomedicine (Doutoramento)
Major in Molecular, Cellular and Developmental Biology
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
"Understanding the Mechanisms of Ageing and Age Reversal" (THESIS/DISSERTATION)
2017/08 - 2019/07
Concluded
 Cellular and Molecular Biology (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Modulation of complex I and oxidative capacity in cells under metabolic stress: the interplay between miR-378a-3p and metformin" (THESIS/DISSERTATION)
 19 valores
2014/09 - 2017/06
Concluded
 Biochemistry (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
 17 valores
Affiliation

Science

Category
Host institution 		Employer
2021/10 - 2022/10 Visiting Researcher (Research) Harvard Medical School, United States
2019/10 - 2020/12 Researcher (Research) Universidade de Coimbra, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Outputs

Publications

Book chapter
  1. Machado, Ivo F.; Miranda, Raul G.; Teodoro, João S.; Dorta, Daniel J.; Rolo, Anabela P.; Palmeira, Carlos M.. "Mitochondrial membrane potential fluctuations associated with the metabolic states of mitochondria". In Methods in Molecular Biology, 117-131. Springer US, 2025.
    Published • 10.1007/978-1-0716-4264-1_7
  2. Palmeira, Carlos M.; Machado, Ivo F.; Ramalho-Santos, João. "Mitochondrial dysfunction in reproductive and developmental toxicity". In Reproductive and Developmental Toxicology, 1103-1116. Elsevier, 2022.
    Published • 10.1016/b978-0-323-89773-0.00054-0
  3. Teodoro, João S.; Machado, Ivo F.; Castela, Ana C.; Rolo, Anabela P.; Palmeira, Carlos M.. "Mitochondria as a target for safety and toxicity evaluation of nutraceuticals". In Nutraceuticals: Efficacy, Safety and Toxicity, edited by Gupta, Ramesh C., 463-483. United States: Academic Press/Elsevier, 2021.
    Published • 10.1016/B978-0-12-821038-3.00030-6
  4. Teodoro, João S.; Machado, Ivo F.; Palmeira, Carlos M.; Rolo, Anabela P.. "Determination of oxidative phosphorylation complexes activities". In Methods in Molecular Biology, 17-31. Springer US, 2021.
    10.1007/978-1-0716-1433-4_2
  5. Machado, Ivo F.; Teodoro, João S.; Castela, Ana C.; Palmeira, Carlos M.; Rolo, Anabela P.. "Mitohormesis". In Mitochondrial Physiology and Vegetal Molecules, 729-746. Elsevier, 2021.
    Published • 10.1016/b978-0-12-821562-3.00035-6
  6. Teodoro, João S.; Machado, Ivo F.; Castela, Ana C.; Rolo, Anabela P.; Palmeira, Carlos M.. "The evaluation of mitochondrial membrane potential using fluorescent dyes or a membrane-permeable cation (TPP+) electrode in isolated mitochondria and intact cells". In Immunometabolism. Methods in Molecular Biology, edited by Mishra, Suresh, 197-213. United States: Springer US, 2020.
    Published • 10.1007/978-1-0716-0802-9_14
Conference abstract
  1. Tian, X.; Li, J.; Wang, B.A.; Griffin, P.; Yang, J.H.; Maybury-Lewis, S.; Lu, Y.; et al. "Epigenetic reprogramming reverses neuronal aging and improves cognitive performance". Paper presented in Mechanisms of Aging, 2022.
  2. Machado, Ivo F.; Teodoro, João S.; Palmeira, Carlos M.; Rolo, Anabela P.. "Interplay between metformin and miR-378a-3p in cells under hyperglycaemia". Paper presented in 12th MiP/MitoEAGLE Training School, 2019.
    10.26124/mitofit:ea19.mipschool.0008
  3. Teodoro, João S.; Rolo, Anabela P.; Da Silva, Rui T.; Machado, Ivo F.; Rosello-Catafau, Joan; Steegborn, Clemens; Palmeira, Carlos M.. "Novel avenues of ischemia/reperfusion injury prevention: lessons from a soluble adenylyl cyclase inhibitor and a SirT3 activator". Paper presented in 53rd Annual Scientific Meeting of the European Society for Clinical Investigation (ESCI), 2019.
    10.1111/eci.13108
Conference poster
  1. Petty, C.A.; Yang, J.H.; Dixon-McDougall, T.; Pichardo, I.; Arnold, M.; Tian, X.; Machado, I.F.; et al. "Ameliorating cellular senescence by partial epigenetic reprogramming". Paper presented in Mechanisms of Aging, 2022.
  2. Machado, Ivo F.; Teodoro, João S.; Palmeira, Carlos M.; Rolo, Anabela P.. "Modulation of complex I and oxidative capacity in cells under metabolic stress". Paper presented in 53rd Annual Scientific Meeting of the European Society for Clinical Investigation (ESCI), 2019.
    10.1111/eci.13109
Journal article
  1. Machado, Ivo F.; Palmeira, Carlos M.; Rolo, Anabela P.. "Sestrin2 is a central regulator of mitochondrial stress responses in disease and aging". Ageing Research Reviews 109 (2025): 102762. https://doi.org/10.1016/j.arr.2025.102762.
    Published • 10.1016/j.arr.2025.102762
  2. Miranda, Raul G.; Machado, Ivo F.; Rolo, Anabela P.; Dorta, Daniel J.; Palmeira, Carlos M.. "HepG2 spheroids cultured in alginate microcapsules as a model for exploring mitochondrial and glycolytic metabolism using the Seahorse XFe24 Analyzer". Toxicology Mechanisms and Methods 35 4 (2025): 413-421. https://doi.org/10.1080/15376516.2024.2447740.
    Published • 10.1080/15376516.2024.2447740
  3. Machado, Ivo F.; Miranda, Raul G.; Dorta, Daniel J.; Rolo, Anabela P.; Palmeira, Carlos M.. "Targeting oxidative stress with polyphenols to fight liver diseases". Antioxidants 12 6 (2023): 1212. https://doi.org/10.3390/antiox12061212.
    10.3390/antiox12061212
  4. Machado, Ivo F.; Palmeira, Carlos M.; Rolo, Anabela P.. "Preservation of mitochondrial health in liver ischemia/reperfusion injury". Biomedicines 11 (2023): 948. https://doi.org/10.3390/biomedicines11030948.
    10.3390/biomedicines11030948
  5. Teodoro, João S.; Da Silva, Rui T.; Machado, Ivo F.; Panisello-Roselló, Arnau; Roselló-Catafau, Joan; Rolo, Anabela P.; Palmeira, Carlos M.. "Shaping of hepatic ischemia/reperfusion events: the crucial role of mitochondria". Cells 11 4 (2022): 688-688. https://doi.org/10.3390/cells11040688.
    10.3390/cells11040688
  6. Da Silva, Rui T.; Machado, Ivo F.; Teodoro, João S.; Panisello-Roselló, Arnau; Roselló-Catafau, Joan; Rolo, Anabela P.; Palmeira, Carlos M.. "PEG35 as a preconditioning agent against hypoxia/reoxygenation injury". International Journal of Molecular Sciences 23 3 (2022): 1156-1156. https://doi.org/10.3390/ijms23031156.
    10.3390/ijms23031156
  7. Teodoro, João S.; Machado, Ivo F.; Castela, Ana C.; Amorim, João A.; Jarak, Ivana; Carvalho, Rui A.; Palmeira, Carlos M.; Rolo, Anabela P.. "Chenodeoxycholic acid has non-thermogenic, mitodynamic anti-obesity effects in an in vitro CRISPR/Cas9 model of bile acid receptor TGR5 knockdown". International Journal of Molecular Sciences 22 21 (2021): 11738. https://doi.org/10.3390/ijms222111738.
    10.3390/ijms222111738
  8. Piochi, Luiz F.; Machado, Ivo F.; Palmeira, Carlos M.; Rolo, Anabela P.. "Sestrin2 and mitochondrial quality control: Potential impact in myogenic differentiation". Ageing Research Reviews 67 (2021): 101309. http://dx.doi.org/10.1016/j.arr.2021.101309.
    10.1016/j.arr.2021.101309
  9. Oliveira, Raúl P.; Machado, Ivo F.; Palmeira, Carlos M.; Rolo, Anabela P.. "The potential role of sestrin 2 in liver regeneration". Free Radical Biology and Medicine 163 (2021): 255-267. http://dx.doi.org/10.1016/j.freeradbiomed.2020.12.027.
    Published • 10.1016/j.freeradbiomed.2020.12.027
  10. Machado, Ivo F.; Teodoro, João S.; Castela, Ana C.; Palmeira, Carlos M.; Rolo, Anabela P.. "miR-378a-3p participates in metformin's mechanism of action on C2C12 cells under hyperglycemia". International Journal of Molecular Sciences 22 2 (2021): 541. https://doi.org/10.3390/ijms22020541.
    Published • 10.3390/ijms22020541
  11. Teodoro, João S.; Amorim, João A.; Machado, Ivo F.; Castela, Ana C.; Steegborn, Clemens; Sinclair, David A.; Rolo, Anabela P.; Palmeira, Carlos M.. "The soluble adenylyl cyclase inhibitor LRE1 prevents hepatic ischemia/reperfusion damage through improvement of mitochondrial function". International Journal of Molecular Sciences 21 14 (2020): 4896-4896. https://doi.org/10.3390/ijms21144896.
    Published • 10.3390/ijms21144896
  12. Machado, Ivo F.; Teodoro, João S.; Palmeira, Carlos M.; Rolo, Anabela P.. "miR-378a: a new emerging microRNA in metabolism". Cellular and Molecular Life Sciences 77 10 (2020): 1947-1958. http://dx.doi.org/10.1007/s00018-019-03375-z.
    Published • 10.1007/s00018-019-03375-z
Preprint
  1. Fazendeiro, Beatriz; Machado, Ivo F.; Rolo, Anabela P.; Rodrigues-Santos, Paulo; Ambrósio, António F.; Santos, Paulo F.; Léger, Hélène. "NDR2 kinase regulate microglial metabolic adaptation and inflammatory response: critical role in glucose-dependent functional plasticity". 2025. https://doi.org/10.1101/2025.06.12.659313.
    10.1101/2025.06.12.659313
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/07/08 Participation and oral presentation at the 12th MiP/MitoEAGLE Training School 12th MiP/MitoEAGLE Training School
Universidade de Coimbra Faculdade de Ciencias e Tecnologia (Coimbra, Portugal)

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2023/03/25 - 2023/03/25 Participation in the debate “Cocktail do envelhecimento saudável”, organized as part of the European Day for the Prevention of Cardiovascular Risk and Brain Awareness Week
Other
Fundação Portuguesa de Cardiologia, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Universidade de Coimbra Multidisciplinary Institute of Ageing, Portugal

Câmara Municipal de Coimbra, Portugal
2022 - 2023 Participation in a set of talks “BCM nas escolas” with the goal of conveying the process of science, through theoretical and practical approaches, to high school students
Workshop
Universidade de Coimbra, Portugal
2022/09/27 - 2022/10/01 Participation at the Mechanisms of Aging conference
Conference
Cold Spring Harbor Laboratory, United States
2019 - 2020 Participation in a set of talks “BCM nas escolas” with the goal of conveying the process of science, through theoretical and practical approaches, to high school students
Workshop
Universidade de Coimbra, Portugal
2019/05/22 - 2019/05/24 Participation and poster presentation at the 53rd ESCI meeting
Meeting
 53rd Annual Scientific Meeting of the European Society for Clinical Investigation (ESCI)
European Society for Clinical Investigation, Netherlands
Distinctions

Other distinction

2020 PhD Research Scholarship
Fundação para a Ciência e a Tecnologia, Portugal