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Teresa Crespo is Senior Scientist at iBET, is Head of the Food Safety & Microbiology Laboratory and Quality Assurance Manager of iBET. Teresa Crespo graduated in Biology at Faculty de Ciências of Universidade de Lisboa and holds a PhD in Biological Engineering from Faculdade de Ciências e Tecnologia of Universidade NOVA de Lisboa. The area of expertise in the last 30 year has been microbiology, in particular microbial characterization and ecology of microbial populations and on antibiotic resistance and pathogenicity/virulence mechanisms of bacteria. The areas of research have been food and water quality and safety, food authenticity and, more recently, in bioremediation processes and water treatments. The laboratory team has participated in the design of state-of-the-art conventional microbiology and molecular biology methodologies to evaluate the presence of microbiological hazards. The team has been working with several methodologies such as phenotypic characterization, qPCR, dPCR, Flow Cytometry, Fluorescence in situ hybridization (FISH) and next generation sequencing. Although more dedicated to bacteria, the team has worked with fungi and more recently with viruses and microalgae. CV Highlights: CV Highlights: (i) more than 85 published papers; (ii) author of 8 book chapters; (iii) editor of 1 book; (iv) supervised (co-supervised) 11 PhD students (thesis defended) and 2 PhD students (ongoing); (v) hosted 9 post-docs; (vi) participated and/or been principal investigator of national and international projects in more than 40 project*; (vii) gave more than 30 conferences oral; (viii) participated in 4 TV programs on science; (ix) contributed as referee for several scientific publications; (x) presented more than 100 posters in national and international conferences (xi) participated in preparation of Guides for Microbiological Laboratories and (xii) is auditor for accredited food microbiological laboratories. *Has participated and been responsible for national projects from Junta Nacional de Investigação Científica e Tecnológica (JNICT), Fundação para a Ciência e Tecnologia and Agência de Inovação (ANI). Has participated and been responsible for iBET participation in projects from FP6 (EU), FP7 (EU), Horizon 2020 (EU). Current research: focused on microbiological aspects of food and water quality and safety (bacteria, viruses and fungi), food authenticity (molecular biology), on the study of antimicrobial activities of bioactive compounds, food biotechnology (microalgae). Other areas of activity: Moreover, has long track-record (since 1998) of working in laboratories with Quality Systems in place. First working under Good Laboratory Practices (GLP) (according to OECD) and actually as Quality Assurance Manager of the Analytical services Unit (ASU) of iBET recognized by Portuguese authorities as working under Good Manufacturing Practices.
Identification

Personal identification

Full name
Maria Teresa Ferreira de Oliveira Barreto Goulão Crespo

Citation names

  • Maria Teresa Barreto Crespo
  • MT Barreto Crespo
  • MT B Crespo

Author identifiers

Ciência ID
BB10-38C7-2CEE
ORCID iD
0000-0002-5605-1419

Telephones

Telephone
  • 214469551 (Professional)

Addresses

  • Av. da Republica, Quinta do Marques, 2780-157, Oeiras, Oeiras, Portugal (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology

Languages

Language Speaking Reading Writing Listening Peer-review
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Advanced (C1) Advanced (C1)
French Advanced (C1) Advanced (C1) Intermediate (B1) Advanced (C1) Elementary (A2)
Portuguese (Mother tongue)
Spanish; Castilian Intermediate (B1) Upper intermediate (B2) Intermediate (B1) Intermediate (B1) Elementary (A2)
Education
Degree Classification
1986 - 1992
Concluded
Engenharia Biológica (Doutoramento)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Aprovado
1977 - 1982
Concluded
Biologia (Licenciatura)
Major in Biologia
Universidade de Lisboa Faculdade de Ciências, Portugal
17
Affiliation

Science

Category
Host institution
Employer
1990 - Current Principal Investigator (Research) Instituto de Biologia Experimental e Tecnológica, Portugal

Teaching in Higher Education

Category
Host institution
Employer
1984 - 1990 Assistant (University Teacher) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal

Others

Category
Host institution
Employer
2015/03 - 2022/07 Quality Assurance Manager of Analytical Services Unit Instituto de Biologia Experimental e Tecnológica, Portugal
2008 - 2015 Director Analytical Services Unit (working under Good Laboratory Practices, OECD and later Good Manufacturing Practices) Instituto de Biologia Experimental e Tecnológica, Portugal
1983 - 1984 High School Teacher Escola Secundária dos Olivais-Chelas, Portugal
Projects

Grant

Designation Funders
2018 - 2021 barcodingPTplants - DNA barcoding aromatic, medicinal, and condiment plants from Portugal
31074
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2021 AgriWwater – Reclaimed wastewater for agriculture irrigation: mitigating microbiological hazards for a safer food production
nº 29586
nº 29586
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2020 MobFood – Mobilização de conhecimento científico e tecnológico em resposta aos desafios do mercado agroalimentar - TC responsible for iBET participation
Programa Operacional Competitividade e Internacionalização; Programa Operacional Regional de Lisboa, 10/SI/2016
Instituto de Biologia Experimental e Tecnológica, Portugal
Ongoing
2012/02 - 2015/07 BIOTEXTILE - Sequencing batch reactor biotechnology toward effective textile wastewater treatment
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2012/02 - 2015/01 Controlling greenhouse gas emissions from biological wastewater treatment systems
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2009 - 2013 BIOSAFE - - BIOlogical agents: Strenghening the Adequate response to deliberate releases by establishment of a Framework European-wide - TC responsible for iBET participation
Contract 022725/2006
N/A
2007/11 - 2011/10 Biological removal of xenobiotics from wastewater treatment systems Fundação para a Ciência e a Tecnologia
2007/10 - 2011/01 Virulence of food enterococci: adding new peaces to the puzzling subject of enterococci as opportunistic pathogens
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2007 - 2011 Development and validation of integrated drinking water treatment processes in Portugal and Norway
PT0012
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2007/01 - 2009/05 Antibiotic stress by vancomycin on enterococci: risk assessment of food safety
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2005 - 2008 ALCUE - From European fork to Latin American farm: an innovative networking platform for EU-LAC partnerships in food quality and safety R&D - TC responsible for iBET participation
007116/2005
007116/2005
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
N/A
Concluded
2005/09 - 2007/12 METABOLISM and CHARACTERIZATION OF MIXED CULTURES IN WASTEWATER PROCESSES FOR SIMULTANEOUS REMOVAL OF NITROGEN and PHOSPHORUS Fundação para a Ciência e a Tecnologia
2004 - 2007 Searching for rhizobial strains with improved skills to thrive in arid soils - TC responsible for iBET participation
POCTI/AGR/46371/2002
POCTI/AGR/46371/2002
Contract CT-2004-506386
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2002 - 2005 Caracterização do catabolismo de péptidos em bactérias lácticas de vinho e produtos cárneos - TC responsible for iBET participation
POCTI/AGR/39613/2001
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
1999 - 2002 Deepening the knowledge on the role of enterococci, from manufacture through maturation of traditional cheeses - TC responsible for iBET participation
POCTI/AGR/36165/1999
POCTI/AGR/36165/1999
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
1996 - 1999 IMPACTO-Investigação dirigida ao Melhoramento do Processo de produção de queijo SerrA por integração de abordagens Científicas e TecnOlógicas - TC responsible for iBET participation
PRAXIS/2/2.1/BIO/1121/95
Fundação para a Ciência e a Tecnologia
1996 - 1998 Técnicas Moleculares na Identificação, Diferenciação e Taxonomia de Microrganismos: Aplicação ao Estudo de Bactérias Heterolácticas e Fungos Fitopatogénicos - TC responsible for iBET participation
PRAXIS/2/2.1/BIA/309/94
PRAXIS/2/2.1/BIA/309/94
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
1993 - 1995 Bactérias Lácticas de Produtos Cárneos Portugueses: Isolamento, Caracterização e Produção de Culturas de Arranque
PBIC/C/AGR/1828/92
Principal investigator
Junta Nacional de Investigação Científica e Tecnológica (JNICT)

Contract

Designation Funders
2022/07 - 2025/12 TEC4GREEN
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
PRR - Plano de Recuperação e Resiliência
Ongoing
2020/05/01 - 2025/04/30 MULTI-STR3AM - A sustainable multi-strain, multi-method, multi-product microalgae biorefinery integrating industrial side streams to create high-value products for food, feed and fragrances
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
H2020 - Bio-based Industries Joint Technology Initiative (BBI-JTI)
Ongoing
2022/03/01 - 2025/02/28 Understanding the mechanisms of inactivation of ultraviolet light emitting diodes to develop effective water disinfection systems
PTDC/EAM-AMB/1561/2021
Instituto de Biologia Experimental e Tecnológica, Portugal

iNOVA4Health, Portugal

Universidade NOVA de Lisboa, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade Nova de Lisboa Microbiologia Molecular Estrutural e Celular, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2023/03/12 - 2024/09/11 Evaluate the effectiveness and effects of ultraviolet light-emitting diodes for food production, preservation, and microbiological safety
2022.01340.PTDC
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2023/03/01 - 2024/08/31 Hunting bacteriophages for food and beverages biopreservation: a novel approach to prevent spoilage by Alicyclobacillus spp.
2022.04043.PTDC
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2021/03/29 - 2024/03/28 PHYCOµBIOME: Understanding and harnessing the power of the microbiome aiming the maximization of marine microalgae productivity
BB10-38C7-2CEE
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2021/01 - 2024/01 CheesOMICs: partindo dos grandes dados, passando pelo laboratório, em direção ao futuro da Denominação de Origem Protegida
Provided by PTCRIS: PTDC/OCE-ETA/1785/2020
Fundação para a Ciência e a Tecnologia
2019/01/01 - 2019/12/31 iNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG and ITQB)
UID/Multi/04462/2019
Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade NOVA de Lisboa NOVA Medical School, Portugal

Universidade NOVA de Lisboa, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal

Universidade Nova de Lisboa Centro de Estudos de Doenças Crónicas, Portugal

Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2017 - 2019 DRafAli – Défis sociétaux et gouvernance des universités africaines – securité alimentaire - - TC responsible for NOVA participation
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Concluded
2017 - 2019 MultiBiorefinery - Estratégias multiuso para a valorização de uma gama alargada de subprodutos agroflorestais e das pescas: Um passo em frente na criação de uma biorrefinaria integrada
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Concluded
2013/12 - 2016/11 DECATHLON - Development of Cost efficient Advanced DNA-based methods for specific Traceability issues and High Level On-site applicatioNs - TC responsible for iBET participation
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
European Commission
Concluded
2012/08 - 2016/07 Collab4Safety - Towards sustainable global food safety collaboration - TC responsible for iBET participation
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
European Commission
Concluded
2012/11 - 2015/10 Early detection and bio-control of mushroom pests and diseases in an Integrated Pest Management approach to comply with the European Directive 2009/128/EU - TC responsible for iBET participation
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
European Commission
Concluded
2011/12 - 2014/05 A Novel and combined domestic grey water treatment and heat recovery system suitable for cost effective installation in 90% of European households
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
European Commission
Concluded
2012 - 2014 CleanWard - Safe, Chemical-Free, Cleaning of Hospital Ward Surfaces
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
FP7-SME - Specific Programme "Capacities": Research for the benefit of SMEs
Concluded
2011/06 - 2013/11 Towards a Latin America & Caribbean Knowledge Based Bio-Economy (KBBE) in partnership with Europe - TC responsible for iBET participation
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
European Commission
Concluded
2010/03 - 2013/08 Sustainable membrane bioreactors for advanced wastewater treatment: a molecular approach
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/03 - 2013/08 Nitrogen-fixing biofertilizers for gramineous crops - TC responsible for iBET participation
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/03 - 2013/06 Fungi Watch: Benefits and hurdles associated with the presence of fungi in drinking water sources
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Khandelwal, P.; Gaspar, F.B.; Crespo, M.T.B.; Upendra, R.S.. "Lactic acid bacteria: General characteristics, food preservation and health benefits". 112-132. 2016.
  2. Gaspar, F.B.; Crespo, M.T.B.. "Lactic acid bacteria as functional starter in food fermentations". 151-169. 2016.
  3. Sanches, S.; Ricardo, J.; Leitão, M.C.; Barreto Crespo, M.T.; Pereira, V.J.. "Determination of polycyclic aromatic hydrocarbons in drinking water sources". 381-390. 2013.
  4. Semedo-Lemsaddek, T.; Alves, P.L.; Tenreiro, R.; Barreto Crespo, M.T.; Barreto Crespo, Maria Teresa; Maria Teresa Barreto Crespo. "Enterococcus". In Molecular Detection of Human Viral Pathogens, edited by Dongyou Liu, 231-248. Boca Raton, United States: CRC Press, 2011.
    Published
Conference abstract
  1. Ana P. Marques; Beatriz Oliveira; João Sério; Jorge Bernardo; Rosa Maria Huertas Penela; Maria Teresa Barreto Crespo; João Paulo Serejo Goulão Crespo; Pereira, Vanessa Jorge . Corresponding author: Pereira, Vanessa Jorge. "Combining ultraviolet light emitting diodes with membrane filtration to develop effective water disinfection systems". Paper presented in Euromembrane 2022, Sorrento, 2022.
    Accepted
  2. Maria Beatriz Cristovão; Ana P. Marques; Andreia Bento-Silva; Maria Gossard; Solomon Tela; João Sério; Maria Rosario Bronze; et al. "Treatment of wastewater effluents using nanofiltration and low pressure UV treatment to produce high quality water that can be reused for irrigation for food production". Paper presented in Euromembrane 2022, Sorrento, 2022.
    Accepted
  3. Carolina Santos; Ana P. Marques; João Sério; Maria Teresa Barreto Crespo; Pereira, Vanessa Jorge . Corresponding author: Pereira, Vanessa Jorge. "Explore light emit diodes and photocatalytic surfaces to prevent Listeria monocytogenes biofilm formation in food industry". Paper presented in Food Micro 2022, Athens, 2022.
    Submitted
  4. Maria Beatriz Cristovão; Andreia Bento da Silva; Solomon Tela; Ana Paula Marques; Maria R. Bonze; Maria Teresa Barreto Crespo; João Paulo Serejo Goulão Crespo; Pereira, Vanessa Jorge. "Wastewater reuse for agriculture irrigation: could nanofiltration be used as tertiary treatment?". Paper presented in Euromembranes 2021, Copenhagen, 2021.
    Published
  5. Leonardo, Inês; Staats, Martijn; Voorhuijzen, Marleen; Vorst, Valerie van der; Fronen, Bas; Capelo, Jorge; Maria Teresa Barreto Crespo; Gaspar, F. B.. "Multilocus DNA metabarcoding of complex mixtures containing members of the Boraginaceae family for food safety and quality assurance". Paper presented in MICROBIOTEC'21, 2021.
  6. Leonardo, Inês C.; Staats, Martijn; Voorhuijzen, Marleen; VAN DER VORST, Valerie; Fronen, Bas; Capelo, Jorge; Maria Teresa Barreto Crespo; Frédéric BUSTOS GASPAR. "Multilocus DNA metabarcoding of complex mixtures containing members of the Boraginaceae family: a step further to assure food safety and quality". Paper presented in ISEKI e-conference 2021 - Food Texture, Quality, Safety and Biosecurity in the Global Bioeconomy, 2021.
  7. Syed, Usman T.; Leonardo, Inês C.; Huertas, Rosa M.; Gaspar, F. B.; Lahoz, Ruth; Maria Teresa Barreto Crespo; Arruebo, Manuel; et al. "Production of temperature-responsive hydrophobic deep eutectic solvent based O/W nanoemulsions by membrane emulsification: Exploratory study on antimicrobial activity". Paper presented in International Congress on Membranes & Membrane Processes, 2020.
Conference paper
  1. Carlota Teles; Lisete Maria Ribeiro de Sousa; Rebouças, Sílvia; Maria Teresa Barreto Crespo; Semedo-Lemsaddek, Teresa. Corresponding author: Carlota Teles. "Microbial diversity and discrimination of Azeitão and Nisa P.D.O. cheeses based on metagenomic data". Paper presented in XXVI Congresso da Sociedade Portuguesa de Estatística, 2023.
Conference poster
  1. Sério, João; Maria Eduarda Martins; Santos, Carolina; Marques, Ana Paula ; Pobre, Vânia; Cecília Arraiano; Serrano, M; et al. "Water disinfection using UVC diodes that emit light at different wavelengths". Paper presented in Congress of Microbiology and Biotechnology (Microbiotec23)., 2023.
  2. Susana Serrano; Semedo-Lemsaddek, Teresa; Catarina Araújo; Maria Teresa Barreto Crespo. "Diversidade microbiana em queijos tradicionais Portugueses com Denominação de Origem Protegida". Paper presented in Microbiologia 2022 - Congresso de Microbiologia em Língua Portuguesa, 2022.
  3. Ana P. Marques; Maria Teresa Barreto Crespo; Pereira, Vanessa Jorge . Corresponding author: Pereira, Vanessa Jorge. "Light-emitting diodes to inactivate foodborne bacteria associated with the fresh produce industry". Paper presented in FoodMicro 2022, 2022.
  4. Leonardo, Inês; Capelo, Jorge; Maria Teresa Barreto Crespo; Gaspar, F. B.. "Molecular detection of species from the Boraginaceae family to safeguard food and feed safety, integrity, authenticity, and quality". Paper presented in MICROBIOTEC'21, 2021.
  5. Oliveira, Micaela; Leonardo, Inês; Maria Teresa Barreto Crespo; Silva, Ana Filipa; Nunes, Mónica. "Antibiotic Resistance Mechanisms in Environmental and Pathogenic Carbapenem Resistant Bacteria from a Portuguese Wastewater Treatment Plant". Paper presented in MICROBIOTEC'21, 2021.
  6. Mónica Nunes; Andreia Filipa-Silva; Maria Teresa Barreto Crespo. "The hidden diversity of viruses in wild and farmed fish species by targeted and untargeted molecular approaches". Paper presented in Anual Meeting in Science, Tecnology and Inovation in Portugal, 2021.
  7. Andreia Filipa-Silva; Maria Teresa Barreto Crespo; Mónica Nunes. Corresponding author: Andreia Filipa-Silva. "Viral diversity of fish from the Atlantic Coast tackled by metagenomics". Paper presented in World Microbe Forum 2021 ¿ An ASM & FEMS Collaboration, 2021.
  8. Andreia Filipa-Silva; Maria Teresa Barreto Crespo; Mónica Nunes. "Fish: a potential source of viral pathogens for humans". Paper presented in Food Integrity Event 2021, 2021.
  9. Andreia Silva; Maria Teresa Barreto Crespo; Nunes, Mónica. "Detection of Hepatitis A, Norovirus GI and GII genomes in wild and farmed fish captured along the Portuguese coast". Paper presented in MicroBiotec'19, 2019.
  10. Micaela Oliveira; Nunes, Mónica; Maria Teresa Barreto Crespo; Ana Filipa Silva. "The fate of carbapenem and quinolone resistant bacteria and genes in two Portuguese full-scale wastewater treatment plants". Paper presented in MicroBiotec'19, 2019.
  11. Leonardo, I C; Gaspar, F. B.; Maria Teresa Barreto Crespo; Pereira, Vanessa Jorge. "Inactivation of Alicyclobacillus spp. in fruit: the potential of medium pressure mercury lamps and light emitting diodes as a first-stage disinfection process in the fruit juice industry". Paper presented in Microbiotec'19, 2019.
  12. Jérôme Antunes; Maria Teresa Barreto Crespo; Ana Filipa Silva; Nunes, Mónica. "Environmental monitoring of enteric viruses in wastewater from two Portuguese wastewater treatment plants". Paper presented in MicroBiotec'19, 2019.
  13. Schneider-Alves V; Cristina Pereira; Rosa Huertas; Jorge Bernardo; João Crespo; Maria Teresa Barreto Crespo; Vanessa J. Pereira; Nunes, Mónica. "Analysis of virus resistance to wastewater disinfection treatments". Paper presented in MicroBiotec'19, 2019.
  14. Nunes, Mónica; Andreia Silva; Ricardo Parreira; Ana Filipa Silva; Maria Teresa Barreto Crespo. "The study of viruses in urban sewage by metagenomics: a tool for public health surveillance". Paper presented in MicroBiotec'19, 2019.
  15. Andreia Silva; Maria Teresa Barreto Crespo; Nunes, Mónica. "First evidence of human Norovirus and Hepatitis A Virus in Wild Fish from the Portuguese Coast". Paper presented in 1º Simpósio INIAV para a Segurança Alimentar Rumo à Alimentação do Futuro, 2019.
  16. Andreia Silva; Maria Teresa Barreto Crespo; Nunes, Mónica. "Detection of pathogenic viruses IPNV, IHNV; VHSV and VNNV in a fast and reliable way: screen for viruses in wild/farmed fish by multiplex qPCR assays". Paper presented in . FEMS - 8th Congress of European Microbiologists, Glasgow, Scotland, 2019.
  17. Micaela Oliveira; Nunes, Mónica; Maria Teresa Barreto Crespo; Ana Filipa Silva. "Widening the view with next generation sequencing and TaqMan multiplex qPCR methods: Detection and quantification of antibiotic resistance genes along the wastewater treatment process". Paper presented in FEMS - 8th Congress of European Microbiologists, Glasgow, Scotland, 2019.
  18. Micaela Oliveira; Nunes, Mónica; Maria Teresa Barreto Crespo; Ana Filipa Silva. "Detection and quantification of antibiotic resistance genes along the wastewater treatment process by TaqMan multiplex qPCR". Paper presented in 15th Symposium on Bacterial Genetics and Ecology (BAGECO 15), Lisbon, Portugal, 2019.
  19. Andreia Silva; Maria Teresa Barreto Crespo; Nunes, Mónica. "Pathogenic foodborne viruses in fish tissues: development of Multiplex TaqMan qPCR assays". Paper presented in 9th ITQB PhD students meeting, Oeiras, Portugal, 2018.
  20. Micaela Oliveira; Nunes, Mónica; Ana Filipa Silva; Maria Teresa Barreto Crespo. "Detection and quantification of antibiotic resistance genes along the wastewater treatment process: optimization of molecular techniques". Paper presented in 9th ITQB PhD students meeting, Oeiras, Portugal, 2018.
  21. Leonardo, I C; Gaspar, F. B.; Maria Teresa Barreto Crespo. "Flow cytometry and conventional microbiology screening of the bioactivity of plant-based natural extracts against Alicyclobacillus acidoterrestris". Paper presented in International Conference on Food Bioactives & Health, 2018.
  22. Andreia Silva; Maria Teresa Barreto Crespo; Nunes, Mónica. "Multiplex TaqMan qPCR assays for sensitive and rapid detection of pathogenic foodborne viruses associated with fish". Paper presented in 5º Simpósio Nacional: Promoção de uma alimentação saudável e segura, Lisbon, Portugal, 2018.
  23. Andreia Silva; Nunes, Mónica; Maria Teresa Barreto Crespo. "Multiplex TaqMan qPCR assays for sensitive and rapid detection of pathogenic foodborne viruses associated with fish and hutting meat". Paper presented in FoodMicro2018, 26th International Conference on Biodiversity of Foodborne Microbes (ICFMH), Berlin, Germany, 2018.
  24. DELGADO, Amélia; Serrano, Maria Cristina; Brito, Maria Dulce; Alves, PI; Fevereiro, Manuel Pedro Salema ; Maria Teresa Barreto Crespo; Peres, Cidália. "Bacteriocins produced by lactic acid bacteria of vegetable origin ¿ have they special features?". Paper presented in XXXIII Congresso Nacional MicroBiotech, 2007.
  25. DELGADO, Amélia; Brito, Maria Dulce; Cidália Peres; Maria Teresa Barreto Crespo; Paula I Alves. "Triagem de bacteriocinas produzidas por bactérias lácticas de azeitona de mesa e seu contributo para a caracterização das especificidades regionais do produto final". Paper presented in IV Simpósio Nacional de Olivicultura, Elvas, 2006.
  26. Cristina I. Pereira; Roma-Rodrigues, Catarina; Nuno Magalhães; Cecília Arraiano; Maria Vitória San Romão; Maria Teresa Barreto Crespo. "Peptides, aminoacids and biogenic amines in Lactobacillus ¿ interactions and metabolism". Paper presented in 1st encounter of the Federation of European Microbiological Societies, 2003.
Journal article
  1. Leonardo, Inês Carvalho; Ferreira, António; Bronze, Maria do Rosário; Quendera, Ana Patrícia; Fernandes, Ana Filipa; Barreto Crespo, Maria Teresa; Bustos Gaspar, Frédéric. "Beyond guaiacol and halophenols: Unravelling isobutyric and isovaleric acids as new culprits in off-flavour spoilage by Alicyclobacillus spp.". International Journal of Food Microbiology 429 (2025): 111002. https://doi.org/10.1016/j.ijfoodmicro.2024.111002.
    10.1016/j.ijfoodmicro.2024.111002
  2. Marques, Ana Paula; Santos, Carolina; Sério, João; Crespo, Maria Teresa Barreto; Pereira, Vanessa Jorge. "Enhancing food safety: Employing ultraviolet-C light emitting diodes for water, leaf, and surface disinfection". Innovative Food Science & Emerging Technologies 98 (2024): 103848. https://doi.org/10.1016/j.ifset.2024.103848.
    10.1016/j.ifset.2024.103848
  3. Serrano, Susana; Ferreira, Maria Vitória; Alves-Barroco, Cinthia; Morais, Susana; Maria Teresa Barreto Crespo; Tenreiro, Rogério; Semedo-Lemsaddek, Teresa. Corresponding author: Semedo-Lemsaddek, Teresa. "Beyond Harmful: Exploring Biofilm Formation by Enterococci Isolated from Portuguese Traditional Cheeses". Foods 13 19 (2024): 3067. https://doi.org/10.3390/foods13193067.
    10.3390/foods13193067
  4. Bertrand, Constança; Martins, Rodrigo; Nunes, Francisco; Brandão, Pedro; Nascimento, Francisco X.. "Genomic insights into indole-3-acetic acid catabolism in the marine algae-associated bacterium, Marinomonas sp. NFXS50". Access Microbiology 6 9 (2024): https://doi.org/10.1099/acmi.0.000856.v3.
    10.1099/acmi.0.000856.v3
  5. Martins, Rodrigo; Bertrand, Constança D.F.; Quintas-Nunes, Francisco; Reynolds-Brandão, Pedro; Barreto Crespo, Maria T.; Nascimento, Francisco X.. "Whole-genome sequences of marine bacteria presenting the ability to promote the growth of the diatom Phaeodactylum tricornutum". Access Microbiology 6 9 (2024): https://doi.org/10.1099/acmi.0.000864.v3.
    10.1099/acmi.0.000864.v3
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  13. Joana Cavadas; Ricardo Parreira; Inês Leonardo; Maria Teresa Barreto Crespo; Mónica Nunes. "Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area". Microorganisms (2022): https://doi.org/10.3390/microorganisms10122443.
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  69. Pereira, V.J.; Marques, M.; Marques, R.; San Romão, M.V.; Barreto Crespo, M.T.. "Occurrence of filamentous fungi in different water sources and their inactivation using chlorine and chloramines". Water Quality Technology Conference and Exposition 2011 (2011): 2133-2135. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873509297&partnerID=MN8TOARS.
  70. Pereira, V.J.; Fernandes, D.; Carvalho, G.; Benoliel, M.J.; San Romão, M.V.; Barreto Crespo, M.T.. "Assessment of the presence and dynamics of fungi in drinking water sources using cultural and molecular methods". Water Research 44 17 (2010): 4850-4859. http://www.scopus.com/inward/record.url?eid=2-s2.0-77957322147&partnerID=MN8TOARS.
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  71. Carlos, A.R.; Semedo-Lemsaddek, T.; Barreto-Crespo, M.T.; Tenreiro, R.. "Transcriptional analysis of virulence-related genes in enterococci from distinct origins". Journal of Applied Microbiology 108 5 (2010): 1563-1575. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950658716&partnerID=MN8TOARS.
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  73. Pereira, C.I.; Matos, D.; San Romão, M.V.; Barreto Crespo, M.T.. "Dual role for the tyrosine decarboxylation pathway in Enterococcus faecium E17: Response to an acid challenge and generation of a proton motive force". Applied and Environmental Microbiology 75 2 (2009): 345-352. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149387437&partnerID=MN8TOARS.
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  74. Carlos, A.R.; Santos, J.; Semedo-Lemsaddek, T.; Barreto-Crespo, M.T.; Tenreiro, R.. "Enterococci from artisanal dairy products show high levels of adaptability". International Journal of Food Microbiology 129 2 (2009): 194-199. http://www.scopus.com/inward/record.url?eid=2-s2.0-59249092223&partnerID=MN8TOARS.
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  75. Alves, P. I. L.; Crespo, M. T. B.; Dourado, A. C.; Fareleira, P.; Ferreira, E. M.; Tenreiro, R.; Tenreiro, T.. "Identification of Sinorhizobium (Ensifer) medicae based on a specific genomic sequence unveiled by M13-PCR fingerprinting". International Microbiology 12 4 (2009): 215-225.
    10.2436/20.1501.01.101
  76. Pereira, C.I.; San Romão, M.V.; Lolkema, J.S.; Barreto Crespo, M.T.. "Weissella halotolerans W22 combines arginine deiminase and ornithine decarboxylation pathways and converts arginine to putrescine". Journal of Applied Microbiology 107 6 (2009): 1894-1902. http://www.scopus.com/inward/record.url?eid=2-s2.0-72849144325&partnerID=MN8TOARS.
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  77. Gaspar, F.; Teixeira, N.; Rigottier-Gois, L.; Marujo, P.; Nielsen-LeRoux, C.; Crespo, M.T.B.; Lopes, M. de F.S.; Serror, P.. "Virulence of Enterococcus faecalis dairy strains in an insect model: The role of fsrB and gelE". Microbiology 155 11 (2009): 3564-3571. http://www.scopus.com/inward/record.url?eid=2-s2.0-70450227165&partnerID=MN8TOARS.
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  78. Semedo-Lemsaddek, T.; Barreto-Crespo, M.T.; Tenreiro, R.. "Occurrence of putative pathogenicity islands in enterococci from distinct species and of differing origins". Applied and Environmental Microbiology 75 22 (2009): 7271-7274. http://www.scopus.com/inward/record.url?eid=2-s2.0-70449337169&partnerID=MN8TOARS.
    10.1128/AEM.00687-09
  79. Pereira, V.J.; Basílio, M.C.; Fernandes, D.; Domingues, M.; Paiva, J.M.; Benoliel, M.J.; Crespo, M.T.; San Romão, M.V.. "Occurrence of filamentous fungi and yeasts in three different drinking water sources". Water Research 43 15 (2009): 3813-3819. http://www.scopus.com/inward/record.url?eid=2-s2.0-67749127714&partnerID=MN8TOARS.
    10.1016/j.watres.2009.05.024
  80. Gaspar, F.B.; Crespo, M.T.B.; Lopes, M.F.S.. "Proposal for a reliable enterococcal cytolysin production assay avoiding apparent incongruence between phenotype and genotype". Journal of Medical Microbiology 58 8 (2009): 1122-1124. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650725848&partnerID=MN8TOARS.
    10.1099/jmm.0.006361-0
  81. Serra, A.T.; Matias, A.A.; Nunes, A.V.M.; Leitão, M.C.; Brito, D.; Bronze, R.; Silva, S.; et al. "In vitro evaluation of olive- and grape-based natural extracts as potential preservatives for food". Innovative Food Science and Emerging Technologies 9 3 (2008): 311-319. http://www.scopus.com/inward/record.url?eid=2-s2.0-44149110650&partnerID=MN8TOARS.
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  82. Pimentel, L.L.; Semedo, T.; Tenreiro, R.; Crespo, M.T.B.; Pintado, M.M.E.; Malcata, F.X.. "Assessment of safety of enterococci isolated throughout traditional terrincho cheesemaking: Virulence factors and antibiotic susceptibility". Journal of Food Protection 70 9 (2007): 2161-2167. http://www.scopus.com/inward/record.url?eid=2-s2.0-34748833520&partnerID=MN8TOARS.
  83. Ribeiro, T.; Abrantes, M.; Lopes, M.d.F.S.; Crespo, M.T.B.. "Vancomycin-susceptible dairy and clinical enterococcal isolates carry vanA and vanB genes". International Journal of Food Microbiology 113 3 (2007): 289-295. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846280170&partnerID=MN8TOARS.
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  85. Silva Lopes, M.D.F.; Ribeiro, T.; Abrantes, M.; Figueiredo Marques, J.J.; Tenreiro, R.; Barreto Crespo, M.T.. "Antimicrobial resistance profiles of dairy and clinical isolates and type strains of enterococci". International Journal of Food Microbiology 103 2 (2005): 191-198. http://www.scopus.com/inward/record.url?eid=2-s2.0-23144461313&partnerID=MN8TOARS.
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  87. Semedo T; Almeida Santos M; Martins P; Silva Lopes MF; Figueiredo Marques JJ; Tenreiro R; Barreto Crespo MT; et al. "Comparative study using type strains and clinical and food isolates to examine hemolytic activity and occurrence of the cyl operon in enterococci.". Journal of clinical microbiology 41 6 (2003): 2569-2576. http://europepmc.org/abstract/med/12791882.
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  88. Oliveira, A.C.; Peres, C.M.; Correia Pires, J.M.; Silva Pereira, C.; Vitorino, S.; Figueiredo Marques, J.J.; Barreto Crespo, M.T.; San Romão, M.V.. "Cork stoppers industry: Defining appropriate mould colonization". Microbiological Research 158 2 (2003): 117-124. http://www.scopus.com/inward/record.url?eid=2-s2.0-0041848582&partnerID=MN8TOARS.
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    10.1007/bf01024514

Other

Other output
  1. Enterococcus faecalis V583 LuxS/AI-2 system is devoid of role in intra-species quorum-sensing but contributes to virulence in a Drosophila host model. 2018. Gaspar F; Teixeira N; Montero N; Aleksandrzak-Piekarczyk T; Matos R; Gonzalez-Zorn B; Jacinto A; Barreto Crespo MT; Silva Lopes MdF. http://europepmc.org/abstract/PPR/PPR9882.
    10.1101/344176
Activities

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2019 - 2023 Wastewater reuse for agricultural irrigation: a comprehensive approach to the mitigation of the antibiotic resistance hazard
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017 - 2021 Unravelling foodborne viruses from fish matrices: evaluation of its potential food safety hazard
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2016 - 2020 Treatment of surface water inoculated with Aspergillus species using ultraviolet radiation and photocatalytic membrane reactors
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2016 - 2019 Investigation of the N2O emissions in wastewater treatment plants, using a molecular, analytical and engineering approach
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2013 Micropollutant removal in wastewater treatment systems: from microbial population structure to function
Supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2013 Integration of membrane filtration and phtolysis processes for drinking water treatment
Supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2013 Microbiology of membrane bioreactors for wastewater treatment: a molecular approach
Supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2008 - 2012 Non-clinical isolates bring new findings on enterococcal virulence
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2007 - 2011 Dynamics of cork mycrobiota throughout stopper manufacturing process: from diversity to metablite production
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2004 - 2008 Peptidolytic System and Bioenergetics of Biogenic Amines Production in Lactic Acid Bacteria
Supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2001 - 2005 Factores de virulência em enterococcus: caracterização fenotípica e molecular de estirpes clínicas e ambientais
Co-supervisor
Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal
1995/02/01 - 1999/01/01 Actividade lipolítica de Lactobacillus: novas perspectivas
Supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal