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Cristiano Mota has been an Assistant Researcher at UCIBIO-NOVA since 2023 (awarded with a contract in the competitive 2022 Individual Scientific Employment Stimulus Competition (CEEC), call promoted by the Fundação para a Ciência e a Tecnologia). He obtained his PhD in Biochemistry from NOVA University Lisbon (2011). His doctoral research focused on the study of molybdoenzymes, employing steady-state kinetics and EPR spectroscopy. In 2012, he was awarded a five-year postdoctoral fellowship at the ESRF in Grenoble, France. During this period, he acquired expertise in X-ray crystallography and SAXS, and served as a Local Contact at the Structural Biology beamlines, providing technical support and scientific guidance to academic and industrial users. In 2017, he joined the Crystallography Group at UCIBIO to investigate molybdoenzymes, specifically aldehyde oxidases and formate dehydrogenases, and their enzymatic mechanisms. He leads the exploratory project PHA4FUTURE and actively contributes as a team member to several other projects (CO2 reduction, Drug Metabolism). To date, he has supervised 1 PhD (ongoing), 5 MSc, and 3 BSc students, and has authored 24 peer-reviewed publications. Cristiano Mota has extensive expertise in molecular biology and heterologous protein production, protein purification, and biochemical and biophysical characterization. Additionally he is highly proficient in structural biology techniques, including X-ray Crystallography and Small Angle X-ray Scattering.
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Identification

Personal identification

Full name
Cristiano De Sousa Mota

Citation names

  • Mota, Cristiano

Author identifiers

Ciência ID
BA13-C0DD-E826
ORCID iD
0000-0002-8999-0420

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
French Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Spanish; Castilian Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2011
Concluded
 Biochemistry (Doutoramento)
Major in Physical Biochemistry
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Characterization of Molybdenum and Tungsten Formate Dehydrogenases from Sulphate Reducing Bacteria" (THESIS/DISSERTATION)
 Unanimidade
2006
Concluded
 Bioquímica (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Estudos bioquímicos e sequenciação de molibdoproteínas de bactérias redutoras de sulfato." (THESIS/DISSERTATION)
 15 (quinze) valores
Affiliation

Science

Category
Host institution 		Employer
2023/05/02 - Current Research Assistant (Research) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Unidade de Ciências Biomoleculares Aplicadas, Portugal
2018/12/20 - 2023/05/01 Researcher (Research) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
(...)
2017/04/01 - 2018/12/19 Postdoc (Research) REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2012/03/01 - 2017/02/28 Postdoc (Research) ESRF, France

Others

Category
Host institution 		Employer
2011/02/01 - 2012/02/28 Postdoctoral researcher REQUIMTE, Portugal
2010/11/01 - 2011/01/31 PhD student (BI) REQUIMTE, Portugal
2006/10/01 - 2010/09/30 PhD student Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2005/09/01 - 2006/07/31 Trainee Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Projects

Contract

Designation Funders
2021/01/01 - 2025/12/31 Institute for Health and Bioeconomy
LA/P/0140/2020
10.54499/LA/P/0140/2020
Researcher
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

INESC Microsistemas e Nanotecnologias, Portugal

Universidade NOVA de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2023/04 - 2025/04 Unveiling the catalytic mechanism of Mo enzymes: Towards efficient carbon dioxide fixation and arsenic detoxification.
2022.15290.CBM
 Fundação para a Ciência e a Tecnologia
2018/12/19 - 2024/12/18 Não disponível
DL 57/2016/CP1379/CT0015
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2021/02/01 - 2024/01/31 Compreender a redução enzimática de CO2 para optimizar catalisadores híbridos
PTDC/BII-BBF/2050/2020
Universidade Nova de Lisboa, Portugal

Universidade Nova de Lisboa Microbiologia Molecular Estrutural e Celular, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/01 - 2023/12/31 Understanding enzymatic CO2 reduction for enhanced hybrid catalysts.
PTDC/BII-BBF/2050/2020
Researcher
Ongoing
2020/01/01 - 2023/12/31 Applied Molecular Biosciences Unit
UIDP/04378/2020
10.54499/UIDP/04378/2020
UIDB/04378/2020
10.54499/UIDB/04378/2020
Researcher
Universidade NOVA de Lisboa, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2022/01 - 2023/06 Structural and biochemical characterization of PHA synthases towards novel Polyhydroxyalkanoates
EXPL/BIA-BQM/1300/2021
EXPL/BIA-BQM/1300/2021
Principal investigator
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2019/01/01 - 2019/12/31 Unidade de Ciências Biomoleculares Aplicadas
UID/Multi/04378/2019
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/04/15 - 2019/10/14 Aldeido Oxidases no Metabolismo de Fármacos e Biocatálise: Em busca da Especificidade do Substrato e Mecanismo Catalitico
PTDC/BBB-BEP/1185/2014
Associação para a Inovação e Desenvolvimento da FCT, Portugal

REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal

Universidade do Porto Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018/10/01 - 2018/12/19 Reduction of CO2 for sustainable biofuel production.
PTDC/BBB-EBB/2723/2014
Post-doc Fellow
Concluded
2017/04/01 - 2018/09/30 Aldehyde oxidase in drug metabolism and biocatalysis
PTDC/BBB-BEP/1185/2014
Post-doc Fellow
Associação para a Inovação e Desenvolvimento da FCT
Concluded
2012/03/01 - 2017/02/28 STRUCTURAL & FUNCTIONAL CHARACTERIZATION OF ENZYMES BELONGING TO THE FAMILY OF PHOSPHOHYDROLSASES FROM DEINOCOCCUS RADIODURANS
SFRH/BEST/51724/2011
 Fundação para a Ciência e a Tecnologia
Concluded
2010/01/01 - 2013/07/15 Proteínas contendo centros heterometálicos envolvidas na resposta a stress
PTDC/QUI-BIQ/098071/2008
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/07/01 - 2012/02/29 Proteins with heterometalic centers involved in stress response
PTDC/QUI-BIQ/098071/2008
Post-doc Fellow
Concluded
2006/10/01 - 2010/09/30 CHARACTERIZATION OF MOLYBDENUM AND TUNGSTEN FORMATE DEHYDROGENASES FROM SULFATE REDUCING BACTERIA
SFRH/BD/32478/2006
 Fundação para a Ciência e a Tecnologia
Concluded
2006/09/01 - 2010/07/31 Incorporação de Mo e/ou W em enzimas
PDCT/QUI/57701/2004
Universidade Católica Portuguesa Portuguesa Centro de Biotecnologia e Química Fina, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2006/10/01 - 2010 Mo and/or W incorporation in enzymes
POCI/QUI/57701/2004
PhD Student Fellow
Concluded
2006/10/01 - 2008 New molybdo and copper cofactors in proteins isolated from sulfate reducers.
POCTI/QUI/55350/2004
PhD Student Fellow
Concluded

Other

Designation Funders
2016/01/01 - 2017/01/31 Structure-function relationship in bacterial regulator of metal acquisition and incorporation
35797UH
Post-doc Fellow
Concluded
Outputs

Publications

Book chapter
  1. Oliveira, Ana Rita; Mota, Cristiano; Romão, Maria; Inês Cardoso Pereira. "The W/SeCys-FdhAB formate dehydrogenase from Desulfovibrio vulgaris Hildenborough". 1-12. Wiley, 2022.
    10.1002/9781119951438.eibc2812
  2. Mota, Cristiano. "Aldehyde Oxidases as Enzymes in Phase I Drug Metabolism". 2019.
    10.1201/9780429295034-13
Conference poster
  1. Vilela-Alves, G; Oliveira, Ana Rita; Rebelo Manuel, Rita; Viegas, Aldino; Carpentier, Philippe; Christophe Léger; Vincent Fourmond; et al. Corresponding author: Mota, Cristiano. "O2 damage on Mo/W Formate dehydrogenases and their innate protection mechanism". Paper presented in 17th European Biological Inorganic Chemistry Conference (EuroBIC-17) (2024), 2024.
  2. Vilela-Alves, G; Rebelo Manuel, Rita; Viegas, Aldino; Philippe Carpentier; Frederic Biaso; Bruno Guigliarelli; Inês A. Cardoso Pereira; Maria Joao Romao; Mota, Cristiano. Corresponding author: Mota, Cristiano. "O2 damage on Mo/W Formate dehydrogenases". Paper presented in 10th National Meeting of Portuguese Users of Synchrotron Radiation, 2024.
  3. Vilela-Alves, G; Oliveira, Ana Rita; Rebelo Manuel, Rita; Viegas, Aldino; Kateryna Klymanska; Philippe Carpentier; Christophe Léger; et al. Corresponding author: Mota, Cristiano. "Unveiling O2 damage on Mo/W Formate dehydrogenases and their innate protection mechanism". Paper presented in Instruct-ERIC Biennial Structural Biology Conference 2024, 2024.
  4. Vilela-Alves, G; Rebelo Manuel, Rita; Oliveira, Ana Rita; Inês A. Cardoso Pereira; Maria Joao Romao; Mota, Cristiano. Corresponding author: Vilela-Alves, G. "Tracking W- Formate Dehydrogenase Structural Changes During Enzyme Reduction and Reoxidation". Paper presented in How Time-Resolved X-ray Crystallography is driving Structural Biology forward Workshop, 2024.
  5. Vilela-Alves, G; Maria Joao Romao; Mota, Cristiano. Corresponding author: Vilela-Alves, G. "Structural studies of bacterial PHA synthases". Paper presented in 8th International School on Biological Crystallization, 2023.
  6. Mota, Cristiano. "Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation". Paper presented in EMBO Workshop Time-resolved spectroscopy meets time-resolved crystallography: The future of dynamic photobiology, 2023.
  7. Oliveira, Ana Rita; Mota, Cristiano; Mourato, Cláudia; Silva, Teresa Santos; Romão, Maria João; Pereira, Inês A.C.. "A highly active oxygen-tolerant w-formate dehydrogenase for CO2 reduction". Paper presented in 12th International conference on hydrogenases and other redox (bio)catalysts for energy conservation, 2019.
Journal article
  1. Guilherme Vilela-Alves; Rita Rebelo Manuel; Neide Pedrosa; Inês A. C. Pereira; Maria João Romão; Cristiano De Sousa Mota. "Structural and biochemical characterization of the M405S variant of Desulfovibrio vulgaris formate dehydrogenase". Acta Crystallographica Section F: Structural Biology Communications 80 Pt 5 (2024): 98-106. https://novaresearch.unl.pt/en/publications/b4f7b8f1-4fcd-4131-9754-1f033b145640.
    10.1107/S2053230X24003911
  2. Cristiano Mota; Myles Webster; Melissa Saidi; Ulrike Kapp; Chloe Zubieta; Gabriele Giachin; José Antonio Manso; Daniele de Sanctis. "Metal ion activation and DNA recognition by the Deinococcus radiodurans manganese sensor DR2539". The FEBS Journal (2024): https://doi.org/10.1111/febs.17140.
    10.1111/febs.17140
  3. Ana Rita Oliveira; Cristiano Mota; Guilherme Vilela-Alves; Rita Rebelo Manuel; Neide Pedrosa; Vincent Fourmond; Kateryna Klymanska; et al. "An allosteric redox switch involved in oxygen protection in a CO2 reductase". Nature Chemical Biology (2024): http://dx.doi.org/10.1038/s41589-023-01484-2.
    10.1038/s41589-023-01484-2
  4. Guilherme Vilela-Alves; Rita R. Manuel; Aldino Viegas; Philippe Carpentier; Frédéric Biaso; Bruno Guigliarelli; Inês A. C. Pereira; Maria João Romão; Cristiano Mota. "Substrate-dependent oxidative inactivation of a W-dependent formate dehydrogenase involving selenocysteine displacement". Chemical Science (2024): https://doi.org/10.1039/D4SC02394C.
    10.1039/D4SC02394C
  5. Cristiano Mota; Ana Rita Oliveira; Rita Rebelo Manuel; Guilherme Vilela-Alves; Inês Cardoso Pereira; Maria João Romão. "One step forward to understand the biological reduction of CO2 to formate by Mo/W formate dehydrogenases". Acta Crystallographica Section A Foundations and Advances (2023): http://dx.doi.org/10.1107/s2053273323091052.
    10.1107/s2053273323091052
  6. Maria Pequito Luís; Inês Serrano Pereira; Joana N. Bugalhão; Catarina N. Simões; Cristiano Mota; Maria João Romão; Luís Jaime Mota. "The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole". Infection and Immunity (2023): http://dx.doi.org/10.1128/iai.00405-22.
    10.1128/iai.00405-22
  7. Guilherme Vilela-Alves; Rita Rebelo Manuel; Ana Rita Oliveira; Inês Cardoso Pereira; Maria João Romão; Cristiano Mota. "Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation". International Journal of Molecular Sciences 24 1 (2022): 476-476. http://dx.doi.org/10.3390/ijms24010476.
    10.3390/ijms24010476
  8. Ana Rita Oliveira; Cristiano Mota; Kateryna Klymanska; Frédéric Biaso; Maria João Romão; Bruno Guigliarelli; Inês Cardoso Pereira. "Spectroscopic and Structural Characterization of Reduced Desulfovibrio vulgaris Hildenborough W-FdhAB Reveals Stable Metal Coordination during Catalysis". ACS Chemical Biology (2022): https://doi.org/10.1021/acschembio.2c00336.
    10.1021/acschembio.2c00336
  9. Cristiano Mota; Ana Diniz; Catarina Coelho; Teresa Santos-Silva; Mariam Esmaeeli; Silke Leimkühler; Eurico J. Cabrita; Filipa Marcelo; Maria João Romão. "Interrogating the Inhibition Mechanisms of Human Aldehyde Oxidase by X-ray Crystallography and NMR Spectroscopy: The Raloxifene Case". Journal of Medicinal Chemistry (2021): https://doi.org/10.1021/acs.jmedchem.1c01125.
    10.1021/acs.jmedchem.1c01125
  10. Mota, Cristiano. "Functional Implications of Second-Shell Basic Residues for dUTPase DR2231 Enzymatic Specificity". ACS Catalysis 10 23 (2020): 13825-13833. http://dx.doi.org/10.1021/acscatal.0c04148.
    10.1021/acscatal.0c04148
  11. Mota, Cristiano. "Toward the Mechanistic Understanding of Enzymatic CO2 Reduction". ACS Catalysis (2020): http://dx.doi.org/10.1021/acscatal.0c00086.
    10.1021/acscatal.0c00086
  12. Cristiano Mota; Mariam Esmaeeli; Catarina Coelho; Teresa Santos-Silva; Martin Wolff; Alessandro Foti; Silke Leimkühler; Maria João Romão. "Human aldehyde oxidase ( hAOX 1): structure determination of the Moco-free form of the natural variant G1269R and biophysical studies of single nucleotide polymorphisms". FEBS Open Bio (2019): https://doi.org/10.1002/2211-5463.12617.
    10.1002/2211-5463.12617
  13. Le Marchand, Tanguy; de Rosa, Matteo; Salvi, Nicola; Sala, Benedetta Maria; Andreas, Loren B; Barbet-Massin, Emeline; Sormanni, Pietro; et al. "Conformational dynamics in crystals reveal the molecular bases for D76N beta-2 microglobulin aggregation propensity.". Nature communications 9 1 (2018): 1658-1658. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:29695721&KeyUID=MEDLINE:29695721.
    10.1038/s41467-018-04078-y
  14. Mota, Cristiano; Coelho, Catarina; Leimkuehler, Silke; Garattini, Enrico; Terao, Mineko; Santos-Silva, Teresa; Romao, Maria Joao. "Critical overview on the structure and metabolism of human aldehyde oxidase and its role in pharmacokinetics". Coordination Chemistry Reviews 368 (2018): 35-59. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000433642700003&KeyUID=WOS:000433642700003.
    10.1016/j.ccr.2018.04.006
  15. Mota, Cristiano S.; Goncalves, Ana Maria D.; de Sanctis, Daniele. "Deinococcus radiodurans DR2231 is a two-metal-ion mechanism hydrolase with exclusive activity on dUTP". FEBS Journal 283 23 (2016): 4274-4290. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000392742500007&KeyUID=WOS:000392742500007.
    10.1111/febs.13923
  16. Gonzalez, Pablo J.; Rivas, Maria G.; Mota, Cristiano S.; Brondino, Carlos D.; Moura, Isabel; Moura, Jose J. G.. "Periplasmic nitrate reductases and formate dehydrogenases: Biological control of the chemical properties of Mo and W for fine tuning of reactivity, substrate specificity and metabolic role". Coordination Chemistry Reviews 257 2 (2013): 315-331. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000312762200002&KeyUID=WOS:000312762200002.
    10.1016/j.ccr.2012.05.020
  17. Mota, Cristiano S.; Valette, Odile; Gonzalez, Pablo J.; Brondino, Carlos D.; Moura, Jose J. G.; Moura, Isabel; Dolla, Alain; Rivas, Maria G.. "Effects of Molybdate and Tungstate on Expression Levels and Biochemical Characteristics of Formate Dehydrogenases Produced by Desulfovibrio alaskensis NCIMB 13491". Journal of Bacteriology 193 12 (2011): 2917-2923. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000291011000002&KeyUID=WOS:000291011000002.
    10.1128/JB.01531-10
  18. Mota, Cristiano S.; Rivas, Maria G.; Brondino, Carlos D.; Moura, Isabel; Moura, Jose J. G.; Gonzalez, Pablo J.; Cerqueira, Nuno M. F. S. A.. "The mechanism of formate oxidation by metal-dependent formate dehydrogenases". Journal of Biological Inorganic Chemistry 16 8 (2011): 1255-1268. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000297227000012&KeyUID=WOS:000297227000012.
    10.1007/s00775-011-0813-8
  19. Rivas, Maria G.; Mota, Cristiano S.; Pauleta, Sofia R.; Carepo, Marta S. P.; Folgosa, Filipe; Andrade, Susana L. A.; Fauque, Guy; et al. "Isolation and characterization of a new Cu-Fe protein from Desulfovibrio aminophilus DSM12254". Journal of Inorganic Biochemistry 103 10 (2009): 1314-1322. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000270795900004&KeyUID=WOS:000270795900004.
    10.1016/j.jinorgbio.2009.04.014
  20. Rivas, Maria G.; Carepo, Marta S. P.; Mota, Cristiano S.; Korbas, Malgorzata; Durand, Marie-Claire; Lopes, Ana T.; Brondino, Carlos D.; et al. "Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis". Biochemistry 48 5 (2009): 873-882. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000263047900009&KeyUID=WOS:000263047900009.
    10.1021/65801773t
  21. Santos, H. M.; Mota, Cristiano; Lodeiro, C.; Moura, Isabel; Isaac, Issa; Capelo, J. L.. "An improved clean sonoreactor-based method for protein identification by mass spectrometry-based techniques". Talanta 77 2 (2008): 870-875. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000261416500067&KeyUID=WOS:000261416500067.
    10.1016/j.talanta.2008.07.048
Online resource
  1. Vilela-Alves, G; Rebelo Manuel, Rita; Inês A. Cardoso Pereira; Maria Joao Romao; Mota, Cristiano. Corresponding author: Mota, Cristiano. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Co-crystallized with Formate and Exposed to air for 0 min [8RC8]. 2024. https://www.rcsb.org/structure/8RC8.
  2. Vilela-Alves, G; Rebelo Manuel, Rita; Inês A. Cardoso Pereira; Maria Joao Romao; Mota, Cristiano. Corresponding author: Mota, Cristiano. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Co-crystallized with Formate and Exposed to air for 2 h [8RC9]. 2024. https://www.rcsb.org/structure/8RC9.
  3. Vilela-Alves, G; Rebelo Manuel, Rita; Inês A. Cardoso Pereira; Maria Joao Romao; Mota, Cristiano. Corresponding author: Mota, Cristiano. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Co-crystallized with Formate and Reoxidized by exposure to air (in a not degassed drop) for 34 min in the presence of Formate [8RCB]. 2024. https://www.rcsb.org/structure/8RCB.
  4. Vilela-Alves, G; Carpentier, Philippe; Rebelo Manuel, Rita; Inês A. Cardoso Pereira; Maria Joao Romao; Mota, Cristiano. Corresponding author: Mota, Cristiano. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - aerobic soaked with 48 bar CO2 for 1 min [8RCC]. 2024. https://www.rcsb.org/structure/8RCC.
  5. Vilela-Alves, G; Mota, Cristiano; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase C872A from Desulfovibrio vulgaris - with Formamide [8CM6]. 2023. https://www.rcsb.org/structure/8CM6.
    https://doi.org/10.2210/pdb8CM6/pdb
  6. Vilela-Alves, G; Mota, Cristiano; Kateryna Klymanska; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase C872A from Desulfovibrio vulgaris [8CM5]. 2023. https://www.rcsb.org/structure/8CM5.
    https://doi.org/10.2210/pdb8CM5/pdb
  7. Vilela-Alves, G; Mota, Cristiano; Kateryna Klymanska; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase C872A from Desulfovibrio vulgaris - exposed to oxygen [8CM4]. 2023. https://www.rcsb.org/structure/8CM4.
    https://doi.org/10.2210/pdb8CM4/pdb
  8. Vilela-Alves, G; Mota, Cristiano; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase M405A from Desulfovibrio vulgaris [8CM7]. 2023. https://www.rcsb.org/structure/8CM7.
    https://doi.org/10.2210/pdb8CM7/pdb
  9. Vilela-Alves, G; Mota, Cristiano; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Soaking with Formate 1 min [8BQG]. 2023. https://www.rcsb.org/structure/8BQG.
  10. Vilela-Alves, G; Mota, Cristiano; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Soaking with Formate 1.5 min [8BQH]. 2023. https://www.rcsb.org/structure/8BQH.
  11. Vilela-Alves, G; Mota, Cristiano; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Soaking with Formate 3 min [8BQI]. 2023. https://www.rcsb.org/structure/8BQI.
  12. Vilela-Alves, G; Mota, Cristiano; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Soaking with Formate 5 min [8BQJ]. 2023. https://www.rcsb.org/structure/8BQJ.
  13. Vilela-Alves, G; Mota, Cristiano; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Soaking with Formate 22 min [8BQK]. 2023. https://www.rcsb.org/structure/8BQK.
  14. Vilela-Alves, G; Mota, Cristiano; Oliveira, Ana Rita; Rebelo Manuel, Rita; Inês Cardoso Pereira; Maria Joao Romao. Structure of W-formate dehydrogenase from Desulfovibrio vulgaris - Co-crystallized with Formate and Reoxidized by exposure to air for 12 min [8BQL]. 2023. https://www.rcsb.org/structure/8BQL.
  15. Vilela-Alves, G; Mota, Cristiano; Maria Joao Romao. Structure of Drosophila melanogaster Aldehyde Oxidase 1 [7PX0]. 2022. https://www.rcsb.org/structure/7PX0.
Preprint
  1. Guilherme Vilela-Alves; Rita R. Manuel; Aldino Viegas; Philippe Carpentier; Frederic Biaso; Bruno Guigliarelli; Inês A. C. Pereira; Maria João Romão; Cristiano Mota. "Substrate-dependent oxidative inactivation of a W-dependent formate dehydrogenase involving selenocysteine displacement". 2024. https://doi.org/10.1101/2024.01.10.571421.
    10.1101/2024.01.10.571421
  2. Cristiano Mota; Myles Webster; Melissa Saidi; Ulrike Kapp; Chloe Zubieta; Gabriele Giachin; José Antonio Manso; Daniele de Sanctis. "Metal ion activation and DNA recognition by theDeinococcus radioduransmanganese sensor DR2539". 2024. https://doi.org/10.1101/2024.02.12.579695.
    10.1101/2024.02.12.579695
Thesis / Dissertation
  1. Mota, Cristiano de Sousa. "Characterization of molybdenum and tungsten formate dehydrogenases from sulfate reducing bacteria". PhD, 2011. http://hdl.handle.net/10362/6688.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/08/29 Metal ion activation and DNA recognition by DR2539, the manganese sensor in Deinococcus radiodurans. 34th European Crystallographic Meeting
(Padova, Italy)
2024/08/28 Oxidative damage on Mo/W Formate dehydrogenases and their innate protection mechanism 34th European Crystallographic Meeting
(Padova, Italy)
2024/05/17 Oxidative damage of Mo/W formate dehydrogenases 2024 Inorganic & Bioinorganic Chemistry Conference
(Lisbon, Portugal)
2023/11/21 Tracking W-Formate Dehydrogenase Structural Changes During Catalysis Advanced Methods for Ambient Crystallography at ESRF-EBS Workshop
European Synchrotron Radiation Facility (Grenoble, France)
2023/08/28 One step forward to understand the biological reduction of CO2 to formate by Mo/W formate dehydrogenases 26th Congress and General Assembly of the International Union of Crystallography
International Union of Crystallography (Melbourne, Australia)
2023/04/17 Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation Virtual EMBO Workshop Time-resolved spectroscopy meets time-resolved crystallography: The future of dynamic photobiology
EMBO
2022/10/18 One step forward to understand the biological reduction of CO2 to Formate by Mo/W Fdhs MoTEC - Molybdenum and Tungsten Enzymes Conference 2022
(Indianapolis, United States)
2021/10/01 The inhibition mechanisms of human aldehyde oxidase by STD-NMR and X-ray crystallography MoTEC - Molybdenum and Tungsten Enzymes Conference
(Marseille)
2020/01/22 Greenhouse gas (CO2) fixation by the formate dehydrogenase (Fdh) enzyme. BioSeminars @UCIBIO
UCIBIO (Lisboa, Portugal)
2019/07/07 Greenhouse gas (CO2) fixation by the formate dehydrogenase (Fdh) enzyme. Iron sulfur for life – Satellite meeting
(Potsdam, Germany)
2019/06/28 The crystal structure of an oxygen- tolerant and highly efficient W-formate dehydrogenase. The 8th Meeting of Portuguese Synchrotron Radiaton Users (ENURS 2019)
(Porto, Portugal)
2019/06/13 The crystal structure of an oxygen- tolerant and highly efficient W-formate dehydrogenase. 1st National Crystallography Meeting
(Aveiro, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2024/09/01 - Current Structural and functional studies of the drug metabolising protein, human aldehyde oxidase
Supervisor of Carolina Dias Videira
 Genética Molecular e Biomedicina (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2023/09/01 - Current Structural, dynamics and mechanistic studies of two enzymatic systems with environmental and biotechnological impact.
Supervisor
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2023/09/01 - Current Chemo-enzymatic PEGs synthesis for enhanced drug delivery
Co-supervisor
2020/09/01 - Current PHA synthases: catalytic mechanism and 3D structure.
Supervisor of Henrique Amado
Bioquímica (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2023/01/23 - 2023/06/19 Finding human aldehyde oxidase interacting partners.
Co-supervisor of Carolina Dias Videira
 Biologia Celular e Molecular (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021/09/01 - 2022/07/28 Unraveling the mode of function of the W-containing formate dehydrogenase (Fdh) enzyme
Supervisor of Guilherme Vilela Alves
 Bioquímica para a Saúde (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/01/01 - 2022/06/30 Biochemical and Structural Characterization of PHA synthases: the future of bioplastics
Supervisor
 Bioquímica (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/09/01 - 2022/02/23 Greenhouse gas (CO2) fixation by the formate dehydrogenase (Fdh) enzyme.
Supervisor of Kateryna Klymanska
 Bioquímica (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/09/01 - 2022/01/28 Biologia estrutural e molecular de uma proteína de virulência de Chlamydia trachomatis.
Co-supervisor of Catarina Neves Simões
Microbiologia Aplicada (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2022 - 2022 Structural studies of a W-formate dehydrogenase by X-ray crystallography – A bacterial enzyme that catalyze greenhouse gas (CO2) fixation
Supervisor
 Bioquímica (Master)
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022 - 2022 Unraveling the mode of function of the W-containing formate dehydrogenase (Fdh) enzyme
Supervisor
 Bioquímica para a Saúde (Master)
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/07/01 - 2020/10/31 PHA sintetase: mecanismo catalítico e estrutura 3D.
Supervisor of Guilherme Vilela Alves
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2020/01/01 - 2020/06/30 Polyhydroxyalkanoate synthase and Aldehyde Oxidase – From the gene to the structure in two related enzymes.
Co-supervisor of Guilherme Vilela Alves
 Química Aplicada (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/01/01 - 2020/06/30 Expressão, purificação e caracterização da proteína Inc CT288 de Chlamydia trachomatis.
Supervisor of Marta Isabel Freitas Vieira
 Biologia Celular e Molecular (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/05/01 - 2020/03/31 A multidisciplinary strategy to study the function of inclusion membrane proteins (Inc) of Chlamydia trachomatis.
Supervisor of Patricia Alexandra Costa Borges
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2019/01/10 - 2019/06/30 Caracterização estrutural e funcional de uma formato desidrogenase de Desulfovibrio vulgaris.
Co-supervisor of Cláudio Alexandre Lopes Colaço
Bioquímica (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/09/01 - 2014/06/30 Mechanistic insights into metal ion activation and operator recognition by the manganese -dependent regulator (DR2539) from Deinococcus radiodurans.
Supervisor of Myles Webster
 Biochemistry (Degree)
University of Bath, United Kingdom

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2024/05/16 - 2024/05/18 2024 Inorganic & Bioinorganic Chemistry Conference (2024/05/16 - 2024/05/18)
Conference (Member of the Organising Committee)
 Sociedade Portuguesa de Química, Portugal
2024/02/22 - 2024/02/22 Workshop: "How Time-Resolved X-Ray Crystallography is driving structural biology forward" (2024/02/22 - 2024/02/22)
Workshop (Member of the Organising Committee)
 Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2024/01/25 SCALE-UP TOOLS FOR THE MANUFACTURE OF HIGH-VALUE BIOCOMPATIBLE POLYMER EXCIPIENTS USING BIOTECHNOLOGY
(Thesis) Main arguer
 Filipe Lopes Estanislau (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2023/12/18 Unraveling the metabolic pathway of dissimilatory sulfate reduction
(Thesis) Main arguer
 Delfim Manuel Araújo Ferreira (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2021/10/27 Structural and Photocatalytic Studies with a High Activity Formate Dehydrogenase
(Thesis) Main arguer
 Diogo Jorge Garça de Lemos Grilo (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Committee member

Activity description
Role 		Institution / Organization
2012 - 2017 The committee - PSB Get Together Activities: Organize 3-4 events/ year in the PSB - Grenoble (ESRF, EMBL, ILL, IBS), promoting science dissemination and networking.
Member
 ESRF, France

Conference scientific committee

Conference name Conference host
2025/05/08 - 2025/05/09 Biophysics Festival 2025 NOVA FCT

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2025/03/05 - 2025/06/30 Bioquimica Estrutural Bioquímica (Mestrado integrado) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2025/03/05 - 2025/06/01 Fundamentos de Bioquímica Estrutural Bioquímica (Licenciatura) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2024/09/01 - 2025/01/31 Enzimologia Bioquímica (Licenciatura) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2024/07/16 - 2024/07/24 Mastering Protein Model Assessment and Preparation (3h) Bioinformatics & Stitch in Biomolecular Sciences (Outros)
2024/07/01 - 2024/07/03 Hands-On: Structure solution by MR methods; refinement and validation of protein complexes (1.5h) Cryo-EM international Summer School and Hands-on 2024 (Outros)
2023/09/01 - 2024/01/31 Enzimologia Bioquimica (Licenciatura) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/09/15 - 2022/12/15 Enzimologia Bioquímica (Licenciatura) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/07/10 - 2022/07/15 Tutor of the Lab Rotation @ 7th European Crystallographic School in Lisbon 7th European Crystallographic School
2022/03/24 - 2022/05/19 Bioquímica Geral B Engenharia Química e Biológica (Licenciatura) Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021/07/28 - 2021/07/28 Macromolecular X-ray crystallography Summer School (Outros) REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2009 - 2009 Bioquímica Analítica Bioquímica (Licenciatura) Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2021/02/01 Disclosing the enzymatic reaction mechanism for the reduction of carbon dioxide. ESRF highlights 2020 booklet

Interview (tv / radio show)

Program Topic
2020/09/28 - 2020/09/28 “90 segundos de ciência” - ANTENA 1 LABORATÓRIO ESTUDA PROTEÍNA CAPAZ DE CONVERTER DIÓXIDO DE CARBONO EM COMBUSTÍVEL

Journal scientific committee

Journal title (ISSN) Publisher
2023 - Current Acta Crystallographica Section F (2053-230X)
2022 - Current Journal of the American Chemical Society (1520-5126) American Chemical Society
2020 - Current Biotechnology letters (0141-5492) Springer Nature
2018/01/01 - 2018/12/31 European Journal of Drug Metabolism and Pharmacokinetics volume

Other jury / evaluation

Activity description Institution / Organization
2024/07/09 - 2024/07/09 Jury member of Biochemistry project (Student: Leonardo Miranda – FCT-NOVA): - Biochemistry Project Report- Expressão, purificação e caracterização de citocromos multihémicos de Cable Bacteria. Work performed at NOVA FCT, 9th of July 2024 Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/06/24 - 2022/06/24 Jury member of Biochemistry project (Student: Catarina Cardadeiro Lavadinho FCT-NOVA): - Degree in Biochemistry Report- Production and crystallization of recombinant wild-type human cystathionine ¿-lyase and C229S and C255S variants. Work performed at ITQB (Oeiras) Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/06/27 - 2019/06/27 Jury member of Biochemistry project (Student: Inês Oliveira Justo – FCT-NOVA): - Biochemistry Project Report- Unravelling protein structure and function using X-ray diffraction. Work performed at EMBL Hamburg, Germany. 27th of June 2019 Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/06/21 - 2018/06/21 Jury member of Biochemistry project (Student: Joana Isabel Passos Dias - FCT-NOVA): - Degree in Biochemistry Report- Isolamento e caracterização bioquímica da Multicopper Oxidase de Enterococcus faecium. Work performed at FCT-NOVA Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Distinctions

Award

2014 Best Poster Award - The 1st FEBS-INSTRUCT crystallization course in the middle EU, advanced methods in macromolecular crystallization VI, Academic and University Center at Nové Hrady, Czech Republic (June 20 - 27, 2014)