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Daniela P. de Freitas is a PhD student in Biochemistry (Faculty of Sciences of the University of Lisbon, FCUL) with a MSc degree in Molecular Biology and Genetics (defended in 2023, Thesis grade: 20 points; final degree grade: 19 points) from the University of Lisbon (ULisboa), having pursued her Dissertation project at the Protein Misfolding and Amyloids in Biomedicine (PMAB) laboratory, affiliated to the Biosystems & Integrative Sciences Institute (BioISI) at FCUL. She obtained her Licentiate degree (BSc) in Biology in 2018 from the Central University of Venezuela, UCV (final score 19/20; recognized by ULisboa as a Licentiate degree with a score of 19/20), where she developed her Licentiate Thesis work on the effects of combinations of different compounds and Benznidazole on Trypanosoma cruzi (final grade: 20 points), at the Biophysics Laboratory of the Experimental Biology Institute (IBE. Caracas, Venezuela), under the supervision of Prof. Vincenza Cervino, while also collaborating on researching the proteolytic activity of the exoproteome of Leishmania mexicana at the Membrane Physiology Laboratory (IBE), under the supervision of Prof. María C. Pérez-Gordones; both works being shared on various conferences. Her main interest lies on life at the molecular level; for that reason, in order to improve her research and analytical skills after a work-focused year in Spain, she decided to pursue her MSc on Molecular Biology and Genetics at ULisboa, which gave her the opportunity to develop her Thesis work on molecular neurodegeneration under Prof. Cláudio M. Gomes supervision, focusing on investigating the extracellular regulation of Tau aggregation and amyloid cross-interactions in Alzheimer's Disease. In 2023, she was awarded an FCT scholarship (2023.03769) to pursue her PhD project on the interplay between aggregation and liquid-liquid phase separation in neurodegeneration, which she is currently developing at the PMAB laboratory, supervised by Prof. Cláudio M. Gomes and co-supervised by Prof. Federico Herrera (BioISI).
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Identification

Personal identification

Full name
Daniela Al Nair José Plasencia de Freitas

Citation names

  • de Freitas, Daniela P.
  • Plasencia, Daniela

Author identifiers

Ciência ID
B915-F604-134E
ORCID iD
0000-0002-0338-9336

Email addresses

  • dpfreitas@fc.ul.pt (Professional)

Telephones

Telephone
  • (+351) 217500256 Ext.: 28556 (Professional)

Addresses

  • Faculdade de Ciências, Universidade de Lisboa, Campo Grande 016, 1749-016, Lisboa, Lisboa, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences
  • Natural sciences - Biological Sciences - Biochemistry
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Biophysics

Languages

Language Speaking Reading Writing Listening Peer-review
Spanish; Castilian (Mother tongue)
English Advanced (C1) Proficiency (C2) Advanced (C1) Advanced (C1) Advanced (C1)
Portuguese Advanced (C1) Advanced (C1) Upper intermediate (B2) Advanced (C1) Advanced (C1)
Education
Degree Classification
2023/10/01 - 2027/09/30
Ongoing
 Bioquímica (Doutoramento)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Exploring the mechanistic relationships between protein aggregation and phase separation in neurodegeneration" (THESIS/DISSERTATION)
2020/09 - 2023/01/27
Concluded
 Biologia Molecular e Genética (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
""Extracellular regulation of Tau aggregation and amyloid cross-interactions in Alzheimer's Disease" Grade: 20/20; Supervisor: Prof. Dr. Cláudio M. Gomes. Discussion: Dr. Miguel Medina (CIBERNED, Madrid)" (THESIS/DISSERTATION)
 19
2018/12/14
Concluded
 Licenciatura en Biología (Licence)
Major in Biology (minor in Cell Biology)
Universidad Central de Venezuela Escuela de Biología, Venezuela
""Effect of combinations of Benznidazole and substituted tetrahydroquinolines over Trypanosoma cruzi proliferation and its plasmatic membrane Ca2+-ATPase". Grade: 20/20. Discussion: Prof. Valentina Salas, Prof. Francisco Arvelo. " (THESIS/DISSERTATION)
 18.8737
Affiliation

Science

Category
Host institution 		Employer
2023/04/15 - 2023/11/15 Research Trainee (Research) FCiênciasID Associação para a Investigação e Desenvolvimento de Ciências, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2022/05/27 - 2023/01/23 Research Trainee (Research) Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2016/01 - 2016/12 Research Trainee (Research) Universidad Central de Venezuela, Venezuela
Universidad Central de Venezuela Instituto de Biologia Experimental, Venezuela

Teaching in Higher Education

Category
Host institution 		Employer
2019/01/01 - 2019/02/22 Assistant (University Teacher) Universidad Central de Venezuela, Venezuela
Universidad Central de Venezuela, Venezuela
2018/01/01 - 2018/12/31 Trainee Assistant (University Teacher) Universidad Central de Venezuela, Venezuela
Universidad Central de Venezuela, Venezuela
2016/03 - 2017/07 Tutor (University Teacher) Universidad Central de Venezuela, Venezuela

Other Careers

Category
Host institution 		Employer
2023/02/01 - 2023/04/14 Assistente Técnico (Assistente Técnico) PhD Trials, Portugal

Others

Category
Host institution 		Employer
2021/06/07 - 2021/09/30 Analyst (content reviewer) Accenture, Portugal
2019/04/30 - 2020/08/31 Assistant seller Catchalot, Spain
Projects

Grant

Designation Funders
2023/10 - 2027/09 Exploring the mechanistic relationships between protein aggregation and phase separation in neurodegeneration
100110DFA
 Fundação para a Ciência e a Tecnologia
Outputs

Publications

Conference abstract
  1. de Freitas, Daniela; Sojo, Felipe; Arvelo, Francisco; Cervino, Vincenza. "Effect of combinations of Benznidazole and substituted tetrahydroquinolines over Trypanosoma cruzi proliferation and its plasmatic membrane Ca2+-ATPase". Paper presented in LXVIII Convención Anual de AsoVAC (Asociación Venezolana para el Avance de la Ciencia), Caracas, 2018.
Conference poster
  1. de Freitas, Daniela P.; Tesei, Giulio; Cao, Fan; Lindorff-Larsen, Kresten; Cláudio M. Gomes. Corresponding author: Cláudio M. Gomes. "Insights into Chaperone-Mediated Regulation of Tau Phase Separation by Biochemical and Computational Approaches". Paper presented in FENS-Hertie Winter School: “Protein aggregation and its consequences in neurodegenerative disorders”, 2024.
  2. de Freitas, Daniela P.; Guilherme G. Moreira; António J. Figueira; Cláudio M. Gomes. Corresponding author: de Freitas, Daniela P.. "Exploring Early Extracellular Disease Mechanisms: Cross-Interactions Between Tau and Amyloid ß in Alzheimer's Disease". Paper presented in Instruct-ERIC Biennial Structural Biology Conference, 2024.
  3. de Freitas, Daniela P.; Guilherme G. Moreira; António J. Figueira; Cláudio M. Gomes. "Exploring Early Extracellular Disease Mechanisms: Cross-interactions Between Tau and Amyloid Beta in Alzheimer's Disease". Paper presented in AD/PD 2024, 2024.
  4. de Freitas, Daniela P.; Guilherme G. Moreira; António J. Figueira; Cláudio M. Gomes. Corresponding author: de Freitas, Daniela P.. "Exploring Early Disease Mechanisms: Cross-Interactions between Aß and Tau in Alzheimer's Disease". Paper presented in 3rd Chem&Biochem Students Meeting, 2023.
  5. Joana V. Ribeiro; Guilherme G. Moreira; António J. Figueira; Mariana Romão; Coelho, Romina; Margarida C. Simões; de Freitas, Daniela P.; et al. "Research on Protein Folding Diseases at the Protein Misfolding and Amyloids in Biomedicine Laboratory". Paper presented in Ciências Research Day 2022, 2022.
  6. de Freitas, Daniela P.; Guilherme G. Moreira; Cláudio M. Gomes. "Extracellular regulation of tau aggregation and amyloid cross-interactions in Alzheimer's disease". Paper presented in 2nd Chem&Biochem Students Meeting, 2022.
  7. de Freitas, Daniela P.; Guilherme G. Moreira; Cláudio M. Gomes. Corresponding author: Cláudio M. Gomes. "Extracellular regulation of Tau aggregation and amyloids cross-interactions in Alzherimer's disease". Paper presented in 2nd Chem & Biochem Students Meeting, 2022.
  8. Mendible-Mendoza, Maria Isabel; Plasencia, Daniela; Romero, Pedro J.; Pérez-Gordones, María Carolina. "Estudio comparativo de la actividad cisteínproteasa en el exoproteoma de promastigotes y amastigotes de Leishmania mexicana". Paper presented in XXV Congreso Latinoamericano de Parasitologia, 2019.
  9. de Freitas, Daniela; Romero, Pedro J.; Pérez-Gordones, María Carolina. "Present proteases in Leishmania mexicana promastigotes exoproteome: comparative study of proteolitic activity in axenic cultures of different subcultures". Paper presented in Extension and Research Journeys of the Faculty of Sciences (UCV), 2018.
Journal article
  1. Daniela P. Freitas; Joana Saavedra; Isabel Cardoso; Cláudio M. Gomes. "Biophysical Studies of Amyloid-Binding Fluorophores to Tau AD Core Fibrils Formed without Cofactors". International Journal of Molecular Sciences (2024): http://dx.doi.org/10.3390/ijms25189946.
    10.3390/ijms25189946
  2. Lucinda Tavares; de Freitas, Daniela; Pedro J. Romero; María Carolina Pérez-Gordones. "In silico characterization of proteases present in the exoproteome of Leishmania mexicana.". Memorias del Instituto de Biología Experimental 9 (2019): 1-4. http://hdl.handle.net/10872/19649.
    Open access • Published
Report
  1. de Freitas, Daniela P.; Cervino, Vincenza. Corresponding author: Cervino, Vincenza. 2016. Determination of the interaction site of diacylglycerol (DAG) and other compounds on the human PMCA..
Thesis / Dissertation
  1. "Extracellular regulation of tau aggregation and amyloid cross-interactions in Alzheimer's disease. Grade: 20/20; Supervisor: Prof. Dr. Cláudio M. Gomes; Discussion: Dr. Miguel Medina (CIBERNED, Madrid)". Master, Universidade de Lisboa Faculdade de Ciências, 2023.
  2. "Efecto de combinaciones de Benznidazol y Tetrahidroquinolinas sustituidas sobre la proliferación de Trypanosoma cruzi y su Ca2+-ATPasa de membrana plasmática. Grade: 20/20 plus Honorific Mention; Supervisor: Prof. Vincenza Cervino. Discussion: Prof. Valentina Salas, Prof. Francisco Arvelo (IBE))". Degree, Universidad Central de Venezuela, 2018.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2018/11/21 Effect of combinations of Benznidazole and substituted tetrahydroquinolines over Trypanosoma cruzi proliferation and its plasmatic membrane Ca2+-ATPase LXVIII Convención Anual de AsoVAC
AsoVAC (Asociación Venezolana para el Avance de la Ciencia) (Caracas, Venezuela)
2018/05/14 Proteasas presentes en el exoproteoma de promastigotes de Leishmania mexicana: Estudio comparativo de la actividad proteolítica en cultivos axénicos con diferente número de pases Jornadas de Investigación y Extensión, Facultad de Ciencias UCV
Universidad Central de Venezuela (Caracas, Venezuela)
2017/05/30 Obtención, amplificación y caracterización mediante enzimas de restricción, de plásmidos pSG5 con formas silvestres y truncadas de la PMCA de humanos
Universidad Central de Venezuela (Caracas, Venezuela)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2024/06/27 - 2024/06/27 4th Chem & Biochem Students Meeting (2024/06/27 - 2024/06/27)
Meeting (Member of the Organising Committee)
 Universidade de Lisboa Departamento de Química e Bioquímica, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2022/11/15 - Current 6º Conferência Anual da RedeSAÚDE
Conference
 6º Conferência Anual da RedeSAÚDE
Universidade de Lisboa, Portugal
2022/02/23 - Current 6th Ulm Meeting — Biophysics of Amyloid Formation (Virtual Meeting)
Meeting
 Biophysics of Amyloid Formation
Universität Ulm, Germany
2021/10 - Current Participation in Weekly Seminars from the Intrinsically Disordered Proteins (IDP) series
Seminar
2015/11/21 - Current Microbiología de Alimentos (Food Microbiology)
Workshop
ChemQuímica, Venezuela
2024/11/28 - 2024/12/03 FENS-Hertie Winter School: “Protein aggregation and its consequences in neurodegenerative disorders”. Obergurgl, Austria.
Workshop
 FENS-Hertie Winter School: “Protein aggregation and its consequences in neurodegenerative disorders”
Federation of European Neuroscience Societies, Belgium
2024/05/13 - 2024/05/17 BLS2: Single molecule biophysics in cell lysates — Hands on training on TIRF-based single molecule imaging in cell lysates and basic image analysis. Vestec, Czechia
Workshop
 BLS2: Single molecule biophysics in cell lysates
Biotechnologický ústav Akademie ved Ceské republiky, Czech Republic
2024/01/15 - 2024/01/18 Training School at the University of Cambridge (UK), organized by Prof. Michele Vendruscolo under the scope of the Twinning Project TWIN2PIPSA.
Workshop
 From pathological protein aggregation to drug discovery
University of Cambridge Yusuf Hamied Department of Chemistry, United Kingdom

TWIN2PIPSA - Twinning for Excellence in Biophysics of Protein Interactions & Protein Self-Assembly, Portugal
2023/12/11 - 2023/12/13 Prediction of variant effects and residue classification via mutational scanning.
Workshop
 TWIN2PIPSA On-site Training - University of Copenhagen partners
TWIN2PIPSA - Twinning for Excellence in Biophysics of Protein Interactions & Protein Self-Assembly, Portugal
2023/12/11 - 2023/12/13 Molecular dynamics studies of IDPs and their sequence-ensemble relationship.
Workshop
 TWIN2PIPSA On-site Training - University of Copenhagen partners
TWIN2PIPSA - Twinning for Excellence in Biophysics of Protein Interactions & Protein Self-Assembly, Portugal
2023/07/13 - 2023/07/13 3rd Chem&Biochem students meeting
Meeting
 3rd Chem&Biochem students meeting
Universidade de Lisboa Faculdade de Ciências, Portugal
2023/04/25 - 2023/04/25 Brain organoids: Modeling neurodevelopment and disease
Seminar
 Brain organoids: Modeling neurodevelopment and disease
Cell Press, United States
2022/07/15 - 2022/07/15 2nd Chem&Biochem students meeting
Meeting
 2nd Chem&Biochem students meeting
Universidade de Lisboa Faculdade de Ciências, Portugal
2021/09/21 - 2021/09/23 Introduction to Image Analysis
Workshop
 Introduction to Image Analysis
Universidade de Lisboa Faculdade de Ciências, Portugal

COLife, Portugal
2018/11/21 - 2018/11/23 LXVIII Convención Anual de AsoVAC
Conference
Asociación Venezolana para el Avance de la Ciencia, Venezuela

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2018/09/17 - Current Laboratorio de Biología Celular (Cell Biology Laboratory) - Practice IIIa (half): Cellular growth (2 weeks) Practice IIIb: Cellular control and regulation; lac operon (4 weeks) Biología (Licence) Universidad Central de Venezuela Escuela de Biología, Venezuela
2018/01 - Current Laboratorio de Bioquímica General (Laboratory of General Biochemistry) Laboratorio de Bioquímica General (Licence) Universidad Central de Venezuela Escuela de Biología, Venezuela
2016/03 - 2017/07 Laboratorio de Biologia Celular (Cell Biology Laboratory) and Laboratorio de Bioquímica General (General Biochemistry Laboratory) (Licence) Universidad Central de Venezuela, Venezuela

Scientific expedition

Activity description Institution / Organization
2024/04/15 - 2024/04/26 Evaluation of the phase separation behaviour of tau and S100B using computational simulations. University of Copenhagen, Denmark. Københavns Universitet - Linderstrøm-Lang Centre for Protein Science, Denmark
Distinctions

Award

2023 PhD Studentship
Fundação para a Ciência e a Tecnologia, Portugal
2022 BII Junior Programme BioISI
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018 Summa cum laude
Universidad Central de Venezuela, Venezuela
2015 Academic Merit Award (1st Place) - Biology School
Central University of Venezuela Facultad de Ciencias, Venezuela
2014 Academic Merit Award (1st Place) - Biology School
Central University of Venezuela Facultad de Ciencias, Venezuela
2013 Academic Merit Award (1st Place) - Biology School
Central University of Venezuela Facultad de Ciencias, Venezuela