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My laboratory is interested on biomolecular and biopharmaceutical engineering aiming the investigation, analysis, and integration of genomic and proteomic data towards the design of therapeutic proteins. This will lead us to guide protein engineering through molecular medicine and bio-nanotechnology in a mid- to long-term timeframe. At the center of our biopharmaceutical development we aim at rational antibody engineering as our approach to validate our theoretical concepts. We consider the use of intracellular and extracellular small-domain antibodies to be exemplary in our rational protein engineering strategies. We have applied our stability engineering strategies to the construction of hyper-stable and effective small antibodies. We want to be able to use our hyperstable frameworks for the construction of small domain antibodies in a number of examples, ranging from HIV to cancer and in model domains to a designed catalytic intrabody. My interest in protein therapeutics may stem the discovery and development of new leads for biopharmaceuticals in the area of infectious diseases, cancer and neurological problems. The focus on the discovery and development of a new class of therapeutic antibodies and efficient display technologies may allow the rapid identification of binding proteins with high affinity and specificity. The domain antibodies combine the advantages of conventional antibodies with the benefits of small-molecule drugs. In addition , we plan to adapt our antibody domain platform to other areas as inhibition of extracellular enzymes and cell surface receptors. Development of better characterization assays and monitoring of antibody therapy in patients, including immunogenicity and pharmacokinetics to biosimilars is current to our work. The evaluation of quality, efficacy and safety attributes of biosimilar antibodies and fusion proteins is at the centre of our clinical collaborations.
Identification

Personal identification

Full name
Joao Goncalves

Citation names

  • Goncalves, Joao

Author identifiers

Ciência ID
B11B-9815-C46F
ORCID iD
0000-0002-1245-3715

Email addresses

  • joao.goncalves@ff.ul.pt (Professional)
  • jgoncalv@ff.ulisboa.pt (Professional)

Telephones

Telephone
  • (351) 217946486 (Professional)

Addresses

  • Faculdade Farmácia Universidade Lisboa, 1649-019, Lisboa, Lisboa, Portugal (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2012
Concluded
Ciencias Farmaceuticas (Título de Agregado)
Major in microbiologia
Universidade de Lisboa Faculdade de Farmácia, Portugal
1996/03/22
Concluded
Ciências Farmacêuticas (Doutoramento)
Major in Virologia
Universidade de Lisboa Faculdade de Farmácia, Portugal
"Functional analysis of HIV-1 Vif protein" (THESIS/DISSERTATION)
1990/03/28
Concluded
Ciências Farmacêuticas (Licenciatura)
Major in microbiologia
Universidade de Lisboa Faculdade de Farmácia, Portugal
Affiliation

Science

Category
Host institution
Employer
2000 - 2003 Visiting Researcher (Research) Scripps Research Institute, United States
Scripps Research Institute, United States
1990 - 1996 Researcher (Research) Harvard Medical School, United States
Dana Farber Cancer Institute, United States

Teaching in Higher Education

Category
Host institution
Employer
2021 - Current Full Professor (University Teacher) Universidade de Lisboa Faculdade de Farmácia, Portugal
Universidade de Lisboa Faculdade de Farmácia, Portugal
2006/03/01 - 2020/12/31 Associate Professor (University Teacher) Universidade de Lisboa Faculdade de Farmácia, Portugal
Universidade de Lisboa Faculdade de Farmácia, Portugal
2004/01/01 - 2005/12/31 Assistant Professor (University Teacher) Universidade de Lisboa Faculdade de Farmácia, Portugal
Universidade de Lisboa Faculdade de Farmácia, Portugal

Other Careers

Category
Host institution
Employer
1998 - 2000 Técnico Superior (Técnico Superior) Merck Sharp & Dohme Corp, United States
Merck Sharp & Dohme Corp, United States
1997 - 1998 Assessor sénior (Farmacêutica) Biologie Servier, France
Biologie Servier, France
Projects

Grant

Designation Funders
2014/05 - 2016/04 Artificial Zinc-Finger transcription factors libraries: Identification of HIV-1 cellular restriction factors and Therapeutic application in HIV-1 infection. Fundação para a Ciência e a Tecnologia, I.P.
2014/03 - 2016/02 An innovative approach to deliver dual-targeting HIV entry inhibitors using cationic liposomes Fundação para a Ciência e a Tecnologia, I.P.
2012/01 - 2014/12 ArmAb: New strategy of ADC for arming antibodies in cancer therapy Fundação para a Ciência e a Tecnologia, I.P.
2013/04 - 2014/09 PhageDuction: a new bacteriophage-based technology for efficient cell-targeted DNA delivery Fundação para a Ciência e a Tecnologia, I.P.
2011/03 - 2014/08 Albumin binding-domain fusions to improve protein pharmacokinetics Fundação para a Ciência e a Tecnologia, I.P.
2009/01 - 2012/06 Biomolecular Engineering Approches for in vitro Production of Self Assembly Viral Nanoparticles Fundação para a Ciência e a Tecnologia, I.P.
2008/03 - 2011/07 Regulation of intracellular anti-viral defense factor APOBEC3G as therapeutic strategy to control HIV-1 infection. Fundação para a Ciência e a Tecnologia, I.P.
2008/01 - 2011/04 A Novel Lentiviral Target-Specific Strategy for Molecular and Gene Therapy Fundação para a Ciência e a Tecnologia, I.P.
2008/03 - 2011/02 Screening the role of kinases/phosphatases in early stages of HIV-1 infection using shRNA-encoded lentiviral libraries. Fundação para a Ciência e a Tecnologia, I.P.
2007/08 - 2011/02 Genome-wide screening with libraries of synthetic zinc-finger proteins capable to modulate host gene expression: Application in HIV-1 infection. Fundação para a Ciência e a Tecnologia, I.P.
2007/01 - 2008/10 Cytidine deaminases as players in intracellular immunity to retroviruses: Mechanisms and Regulation Fundação para a Ciência e a Tecnologia, I.P.
2005/06 - 2008/06 Viral Nanopaticles por Molecular and Gene Therpay Fundação para a Ciência e a Tecnologia, I.P.
2005/05 - 2006/12 Cytidine deaminases as players in intracellular immunity to retroviruses: Mechanisms and Regulation Fundação para a Ciência e a Tecnologia, I.P.
2003/12 - 2006/05 Inhibition of HIV infectivity by single-domain antibodies.
Provided by PTCRIS: 49729
Fundação para a Ciência e a Tecnologia, I.P.
2002/05 - 2004/04 Inhibition of Kaposis's sarcoma-associated herpes virus latency by intracellular antibodies
Provided by PTCRIS: 38229
Fundação para a Ciência e a Tecnologia, I.P.
2001/02 - 2004/01 INTRACELLULAR IMMUNIZATION FOR AIDS THERAPY: TARGETING HIV INFECTIVITY
Provided by PTCRIS: 33096
Fundação para a Ciência e a Tecnologia, I.P.

Contract

Designation Funders
2022/01/01 - 2024/12/31 BREAST-BRAIN-N-BBB
BREAST-BRAIN-N-BBB
Researcher
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
CaixaForum Barcelona
Ongoing
2020/06/01 - 2023/03/31 Testimmuno Covid19: Avaliação da resposta imunitária e virológica em doentes Covid19 e pessoas vacinadas.
AAC 02/SAICT/2020
Principal investigator
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
Fundação para a Ciência e a Tecnologia, I.P.
Ongoing
2018 - 2020 Engineering novel ADC with potent immune functions
PF029145
Principal investigator
Ongoing
2015 - 2016 Drug repositioning: Re-investigating existing drugs for treatment and functional cure of HIV-1 infection
OP23713
Principal investigator
Concluded
Outputs

Publications

Book
  1. Aguiar, S.; Dias, J.; Manuel, A.M.; Russo, R.; Gois, P.M.P.; da Silva, F.A.; Goncalves, J.. Chimeric Small Antibody Fragments as Strategy to Deliver Therapeutic Payloads. 2018.
    10.1016/bs.apcsb.2018.03.002
  2. Côrte-Real, S.; Aires Da Silva, F.; Gonçalves, J.. Development of Antibody Fragments for Therapeutic Applications. 2011.
    10.1002/9783527636730.ch16
Book chapter
  1. Goncalves, Joao. "Therapeutic Antibody Engineering and Selection Strategies". 2019.
    10.1007/10_2019_116
  2. Goncalves, Joao. "Insights on the Formulation of Recombinant Proteins". 2019.
    10.1007/10_2019_119
  3. Goncalves, Joao. "Preclinical and Clinical Development of Biosimilar Antibodies". 2016.
    10.1002/9781118940648.ch17
Conference paper
  1. Condelipes, P.G.M.; Fontes, P.M.; Nikolaidou, K.; Marques, V.; Bras, E.J.S.; Afonso, M.B.; Rodrigues, C.M.P.; et al. "Development of a Microfluidic Colorectal Cancer Cell Culture System with Integrated Optical Sensors for Rapid Phage Selection". 2020.
    10.1109/SENSORS47125.2020.9278738
Conference poster
  1. Ribeiro, Rita; Moreira, João Nuno; Goncalves, Joao. "Development of anti-nucleolin antibody against Triple-Negative Breast Cancer". Paper presented in III ASPICASEICA International Meeting – Cancer Immunology, Tumor Microenvironment and Metastasis, 2023.
  2. Ribeiro, Rita; Moreira, João Nuno; Goncalves, Joao. "Affinity Maturation And Antibody-Dependent Cellular Cytotoxicity Of An Anti-Nucleolin Antibody Against Triple-Negative Breast Cancer". Paper presented in 14th iMed.ULisboa Postgraduate Students Meeting, 2023.
  3. Peres, Carina; Ana I. Matos; Catarina Cunha-Santos, A; Viana, Ana S. or Viana, Ana; Wagner, Angela M.; Vela Ramirez, Julia E.; Goncalves, Joao; et al. "Polymeric nanoparticle-based vaccine to target dendritic cells and the tumor microenvironment". Paper presented in UT Austin - Portugal Program Annual Conference, 2016.
  4. Amaral, Andreia J.; Soares, Rui S.; Gama-Carvalho, Margarida; Goncalves, Joao; Ana Espada de Sousa. "Profiling microRNA expression during human CD4+ T-cell activation". Paper presented in ESF, Next Generation Sequencing Meeting, Leiden, Netherlands, 2010.
Journal article
  1. Barros, Ana Luisa A. N.; Silva, Vladimir C.; Ribeiro-Junior, Atvaldo F.; Cardoso, Miguel G.; Costa, Samuel R.; Moraes, Carolina B.; Barbosa, Cecília G.; et al. "Antiviral Action against SARS-CoV-2 of a Synthetic Peptide Based on a Novel Defensin Present in the Transcriptome of the Fire Salamander (Salamandra salamandra)". Pharmaceutics 16 2 (2024): 190. http://dx.doi.org/10.3390/pharmaceutics16020190.
    10.3390/pharmaceutics16020190
  2. Clare Harris; Richard James Harris; David Young; Martin McDonnell; Bridget Clancy; Justin Harvey; Carlos Araujo; et al. "Clinical Outcomes and Patient Experience of Biosimilar to Biosimilar Infliximab Switching in Patients with Inflammatory Bowel Disease: A Prospective, Single-Centre, Phase IV Interventional Study with a Nested Qualitative Study". GastroHep (2023): https://doi.org/10.1155/2023/1248526.
    10.1155/2023/1248526
  3. Brás, R.; Goncalves, J.; Araújo, C.; Godinho Santos, A.; Costa, L.; Pedro, E.; Caiado, J.. "sIgE/sIgG4 profile in platinum desensitization: is there immunological tolerance?". European Annals of Allergy and Clinical Immunology online fir (2023): http://dx.doi.org/10.23822/eurannaci.1764-1489.302.
    10.23822/eurannaci.1764-1489.302
  4. Simão, André Lopes; Palma, Carolina Santos; Izquierdo-Sanchez, Laura; Putignano, Antonella; Carvalho-Gomes, Angela; Posch, Andreas; Zanaga, Paola; et al. "Cirrhosis is associated with lower serological responses to COVID-19 vaccines in patients with chronic liver disease". JHEP Reports 5 5 (2023): 100697. http://dx.doi.org/10.1016/j.jhepr.2023.100697.
    10.1016/j.jhepr.2023.100697
  5. André, Ana S.; Dias, Joana N. R.; Aguiar, Sandra; Nogueira, Sara; Bule, Pedro; Carvalho, Joana Inês; António, João P. M.; et al. "Rabbit derived VL single-domains as promising scaffolds to generate antibody–drug conjugates". Scientific Reports 13 1 (2023): http://dx.doi.org/10.1038/s41598-023-31568-x.
    10.1038/s41598-023-31568-x
  6. Figueiredo, Diogo; Cascalheira, António; Goncalves, Joao. "Rapid, multiplex detection of SARS-CoV-2 using isothermal amplification coupled with CRISPR-Cas12a". Scientific Reports 13 1 (2023): http://dx.doi.org/10.1038/s41598-022-27133-7.
    10.1038/s41598-022-27133-7
  7. Zvonimir Petric; Joao Goncalves; Paulo Paixao. "Under the Umbrella of Clinical Pharmacology: Inflammatory Bowel Disease, Infliximab and Adalimumab, and a Bridge to an Era of Biosimilars". Pharmaceutics (2022): https://doi.org/10.3390/pharmaceutics14091766.
    10.3390/pharmaceutics14091766
  8. Iria, I.; Soares, R.R.G.; Brás, E.J.S.; Chu, V.; Gonçalves, J.; Conde, J.P.. "Accurate and rapid microfluidic ELISA to monitor Infliximab titers in patients with inflammatory bowel diseases". Analyst 147 3 (2022): 480-488. http://www.scopus.com/inward/record.url?eid=2-s2.0-85123969178&partnerID=MN8TOARS.
    10.1039/d1an01810h
  9. Schreiber, S.; Puig, L.; Gonçalves, J.; Mease, P.J.; Panaccione, R.; Emery, P.. "Critical appraisal and future outlook on anti-inflammatory biosimilar use in chronic immune-mediated inflammatory diseases". Seminars in Arthritis and Rheumatism 55 (2022): http://www.scopus.com/inward/record.url?eid=2-s2.0-85131446689&partnerID=MN8TOARS.
    10.1016/j.semarthrit.2022.152023
  10. Bandarra, Susana; Miyagi, Eri; Ribeiro, Ana Clara; Gonçalves, João; Strebel, Klaus; Barahona, Isabel. "APOBEC3B Potently Restricts HIV-2 but Not HIV-1 in a Vif-Dependent Manner". Journal of Virology 95 23 (2021): http://dx.doi.org/10.1128/jvi.01170-21.
    10.1128/jvi.01170-21
  11. "Highly Specific Blood-Brain Barrier Transmigrating Single-Domain Antibodies Selected by an In Vivo Phage Display Screening". Pharmaceutics 13 10 (2021): 1598-1598. http://dx.doi.org/10.3390/pharmaceutics13101598.
    10.3390/pharmaceutics13101598
  12. "Biosimilars in an era of rising oncology treatment options". Future Oncology 17 29 (2021): 3881-3892. http://dx.doi.org/10.2217/fon-2021-0546.
    10.2217/fon-2021-0546
  13. "Methods and cell-based strategies to produce antibody libraries: current state". Applied Microbiology and Biotechnology 105 19 (2021): 7215-7224. http://dx.doi.org/10.1007/s00253-021-11570-x.
    10.1007/s00253-021-11570-x
  14. "A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent". Separation and Purification Technology 265 (2021): 118476-118476. http://dx.doi.org/10.1016/j.seppur.2021.118476.
    10.1016/j.seppur.2021.118476
  15. "Integrated in Silico and Experimental Approach towards the Design of a Novel Recombinant Protein Containing an Anti-HER2 scFv". International Journal of Molecular Sciences 22 7 (2021): 3547-3547. http://dx.doi.org/10.3390/ijms22073547.
    10.3390/ijms22073547
  16. "Screening polymeric ionic liquids for chromatography-based purification of bacteriophage M13". Separation and Purification Technology 257 (2021): 117906-117906. http://dx.doi.org/10.1016/j.seppur.2020.117906.
    10.1016/j.seppur.2020.117906
  17. "Systematic Review and Principal Components Analysis of the Immunogenicity of Adalimumab.". BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy (2021): https://doi.org/10.1007/s40259-020-00458-3.
    10.1007/s40259-020-00458-3
  18. Cerván-Martín, M.; Bossini-Castillo, L.; Rivera-Egea, R.; Garrido, N.; Luján, S.; Romeu, G.; Santos-Ribeiro, S.; et al. "Effect and in silico characterization of genetic variants associated with severe spermatogenic disorders in a large Iberian cohort". Andrology 9 4 (2021): 1151-1165. http://www.scopus.com/inward/record.url?eid=2-s2.0-85112490115&partnerID=MN8TOARS.
    10.1111/andr.13009
  19. Park, K.; Li, C.; Tsiropoulou, S.; Gonçalves, J.; Kondratev, C.; Pelletier, L.; Blacque, O.E.; Leroux, M.R.. "CDKL kinase regulates the length of the ciliary proximal segment". Current Biology 31 11 (2021): 2359-2373.e7. http://www.scopus.com/inward/record.url?eid=2-s2.0-85105010786&partnerID=MN8TOARS.
    10.1016/j.cub.2021.03.068
  20. Holgersen, E.M.; Gandhi, S.; Zhou, Y.; Kim, J.; Vaz, B.; Bogojeski, J.; Bugno, M.; et al. "Transcriptome-Wide Off-Target Effects of Steric-Blocking Oligonucleotides". Nucleic Acid Therapeutics 31 6 (2021): 392-403. http://www.scopus.com/inward/record.url?eid=2-s2.0-85121681071&partnerID=MN8TOARS.
    10.1089/nat.2020.0921
  21. Vale, F.F.; Vítor, J.M.B.; Marques, A.T.; Azevedo-Pereira, J.M.; Anes, E.; Goncalves, J.. "Origin, phylogeny, variability and epitope conservation of SARS-CoV-2 worldwide". Virus Research 304 (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85111961673&partnerID=MN8TOARS.
    10.1016/j.virusres.2021.198526
  22. Cadima-Couto, Iris; Tauzin, Alexandra; Freire, João M.; Figueira, Tiago N.; Silva, Rúben D. M.; Pérez-Peinado, Clara; Cunha-Santos, Catarina; et al. "Anti-HIV-1 Activity of pepRF1, a Proteolysis-Resistant CXCR4 Antagonist Derived from Dengue Virus Capsid Protein". ACS Infectious Diseases 7 1 (2020): 6-22. http://dx.doi.org/10.1021/acsinfecdis.9b00507.
    10.1021/acsinfecdis.9b00507
  23. Strand, Vibeke; Goncalves, Joao; Isaacs, John D.. "Immunogenicity of biologic agents in rheumatology". Nature Reviews Rheumatology 17 2 (2020): 81-97. http://dx.doi.org/10.1038/s41584-020-00540-8.
    10.1038/s41584-020-00540-8
  24. St Clair-Jones, Anja; Prignano, Francesca; Goncalves, Joao; Paul, Muriel; Sewerin, Philipp. "Understanding and Minimising Injection-Site Pain Following Subcutaneous Administration of Biologics: A Narrative Review". Rheumatology and Therapy 7 4 (2020): 741-757. http://dx.doi.org/10.1007/s40744-020-00245-0.
    10.1007/s40744-020-00245-0
  25. Cascão, Rita; Vidal, Bruno; Carvalho, Tânia; Lopes, Inês Pascoal; Romão, Vasco C.; Goncalves, João; Moita, Luis Ferreira; Fonseca, João Eurico. "Celastrol Efficacy by Oral Administration in the Adjuvant-Induced Arthritis Model". Frontiers in Medicine 7 (2020): http://dx.doi.org/10.3389/fmed.2020.00455.
    10.3389/fmed.2020.00455
  26. Perdigão, Pedro R.L.; Cunha-Santos, Catarina; Barbas, Carlos F.; Santa-Marta, Mariana; Goncalves, Joao. "Protein Delivery of Cell-Penetrating Zinc-Finger Activators Stimulates Latent HIV-1-Infected Cells". Molecular Therapy - Methods & Clinical Development 18 (2020): 145-158. http://dx.doi.org/10.1016/j.omtm.2020.05.016.
    10.1016/j.omtm.2020.05.016
  27. Lüftner, Diana; Lyman, Gary H.; Gonçalves, João; Pivot, Xavier; Seo, Minji. "Biologic Drug Quality Assurance to OptimizeHER2¿+¿Breast Cancer Treatment: Insights from Development of the Trastuzumab BiosimilarSB3". Targeted Oncology 15 4 (2020): 467-475. http://dx.doi.org/10.1007/s11523-020-00742-w.
    10.1007/s11523-020-00742-w
  28. Santos, Raquel dos; Iria, Inês; Manuel, Ana M.; Leandro, Ana P.; Madeira, Catarina A. C.; Goncalves, Joao; Carvalho, Ana Luísa; Roque, Ana Cecília A.. "Magnetic Precipitation: A New Platform for Protein Purification". Biotechnology Journal 15 9 (2020): http://dx.doi.org/10.1002/biot.202000151.
    10.1002/biot.202000151
  29. Goncalves, Joao. "Protein delivery of cell-penetrating zinc-finger activators stimulates latent HIV-1 infected cells". Molecular Therapy - Methods & Clinical Development MTM-D-20-0 Accepted (2020):
  30. Ministro, Joana H.; Oliveira, Soraia S.; Oliveira, Joana G.; Cardoso, Miguel; Aires-da-Silva, Frederico; Corte-Real, Sofia; Goncalves, Joao. "Synthetic antibody discovery against native antigens by CRISPR/Cas9-library generation and endoplasmic reticulum screening". Applied Microbiology and Biotechnology 104 6 (2020): 2501-2512. http://dx.doi.org/10.1007/s00253-020-10423-3.
    10.1007/s00253-020-10423-3
  31. Strand, V.; Gonçalves, J.; Hickling, T.P.; Jones, H.E.; Marshall, L.; Isaacs, J.D.. "Correction to: Immunogenicity of Biosimilars for Rheumatic Diseases, Plaque Psoriasis, and Inflammatory Bowel Disease: A Review from Clinical Trials and Regulatory Documents (BioDrugs, (2020), 34, 1, (27-37), 10.1007/s40259-019-00394-x)". BioDrugs 34 2 (2020): 253-253. http://www.scopus.com/inward/record.url?eid=2-s2.0-85080149855&partnerID=MN8TOARS.
    10.1007/s40259-020-00410-5
  32. Lüftner, D.; Lyman, G.H.; Gonçalves, J.; Pivot, X.; Seo, M.. "Correction to: Biologic Drug Quality Assurance to Optimize HER2+ Breast Cancer Treatment: Insights from Development of the Trastuzumab Biosimilar SB3 (Targeted Oncology, (2020), 15, 4, (467-475), 10.1007/s11523-020-00742-w)". Targeted Oncology 15 6 (2020): 805-805. http://www.scopus.com/inward/record.url?eid=2-s2.0-85092798366&partnerID=MN8TOARS.
    10.1007/s11523-020-00761-7
  33. Ferrasi, A.C.; Fernandez, G.J.; Grotto, R.M.T.; Silva, G.F.; Goncalves, J.; Costa, M.C.; Enguita, F.J.; Pardini, M.I.M.C.. "New LncRNAs in Chronic Hepatitis C progression: from fibrosis to hepatocellular carcinoma". Scientific Reports 10 1 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85086691090&partnerID=MN8TOARS.
    10.1038/s41598-020-66881-2
  34. Tsatskis, Y.; Rosenfeld, R.; Pearson, J.D.; Boswell, C.; Qu, Y.; Kim, K.; Fabian, L.; et al. "The NEMP family supports metazoan fertility and nuclear envelope stiffness". Science Advances 6 35 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85090872373&partnerID=MN8TOARS.
    10.1126/sciadv.abb4591
  35. Gonçalves, J.; Sharma, A.; Coyaud, É.; Laurent, E.M.N.; Raught, B.; Pelletier, L.. "LUZP1 and the tumor suppressor EPLIN modulate actin stability to restrict primary cilia formation". Journal of Cell Biology 219 7 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85086061049&partnerID=MN8TOARS.
    10.1083/JCB.201908132
  36. Goncalves, Joao. "Efficacy, immunogenicity and cost analysis of a systematic switch from originator infliximab to biosimilar CT-P13 of all patients with inflammatory arthritis from a single center.". Acta reumatologica portuguesa (2019):
  37. Goncalves, Joao. "Immunogenicity of Biosimilars for Rheumatic Diseases, Plaque Psoriasis, and Inflammatory Bowel Disease: A Review from Clinical Trials and Regulatory Documents". BioDrugs (2019): http://dx.doi.org/10.1007/s40259-019-00394-x.
    10.1007/s40259-019-00394-x
  38. Goncalves, Joao. "Biosimilars: An Opportunity to Update the Product Information of Biological Drugs Regarding their Immunogenicity.". BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy (2019): https://doi.org/10.1007/s40259-019-00391-0.
    10.1007/s40259-019-00391-0
  39. Goncalves, Joao. "Inhibition of HIV replication through siRNA carried by CXCR4-targeted chimeric nanobody.". Cellular and molecular life sciences : CMLS (2019): https://doi.org/10.1007/s00018-019-03334-8.
    10.1007/s00018-019-03334-8
  40. Goncalves, Joao. "Analysis of Immunogenicity Data in the Product Information of Biological Drugs: A Need to Report Immunogenicity Data Systematically.". BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy (2019): https://doi.org/10.1007/s40259-019-00387-w.
    10.1007/s40259-019-00387-w
  41. Goncalves, Joao. "Abstract Supplement 2019 ACR/ARP Annual Meeting". Arthritis & Rheumatology (2019): http://dx.doi.org/10.1002/art.41108.
    10.1002/art.41108
  42. Goncalves, Joao. "Biosimilars in rheumatology.". Pharmacological research (2019): https://doi.org/10.1016/j.phrs.2019.104467.
    10.1016/j.phrs.2019.104467
  43. Goncalves, Joao. "Nucleolin-based targeting strategies for cancer therapy: from targeted drug delivery to cytotoxic ligands.". Drug discovery today (2019): https://doi.org/10.1016/j.drudis.2019.06.018.
    10.1016/j.drudis.2019.06.018
  44. Goncalves, Joao. "Era of biosimilars in rheumatology: reshaping the healthcare environment.". RMD open (2019): http://europepmc.org/articles/PMC6560670.
    10.1136/rmdopen-2019-000900
  45. Goncalves, Joao. "Anti-TNF biosimilars in psoriasis: from scientific evidence to real-world experience.". The Journal of dermatological treatment (2019): https://doi.org/10.1080/09546634.2019.1610553.
    10.1080/09546634.2019.1610553
  46. Goncalves, Joao. "P664 SB5 and reference adalimumab show cross-immunogenicity in patients with inflammatory bowel disease or rheumatoid arthritis". Journal of Crohn's and Colitis (2019): http://dx.doi.org/10.1093/ecco-jcc/jjy222.788.
    10.1093/ecco-jcc/jjy222.788
  47. Goncalves, Joao. "SB5 shows cross-immunogenicity to adalimumab but not infliximab: results in patients with inflammatory bowel disease or rheumatoid arthritis". Therapeutic Advances in Gastroenterology (2019): http://dx.doi.org/10.1177/1756284819891081.
    10.1177/1756284819891081
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  111. Nolasco, S.; Bellido, J.; Gonçalves, J.; Tavares, A.; Zabala, J.C.; Soares, H.. "The expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisense Tbca RNA during testis maturation". PLoS ONE 7 8 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84864697552&partnerID=MN8TOARS.
    10.1371/journal.pone.0042536
  112. Cadima-Couto, I.; Saraiva, N.; Santos, A.C.C.; Goncalves, J.. "HIV-1 Vif interaction with APOBEC3 deaminases and its characterization by a new sensitive assay". Journal of Neuroimmune Pharmacology 6 2 (2011): 296-307. http://www.scopus.com/inward/record.url?eid=2-s2.0-79957449842&partnerID=MN8TOARS.
    10.1007/s11481-011-9258-7
  113. Esteves, A.I.S.; Nicolai, M.; Humanes, M.; Goncalves, J.. "Sulfated polysaccharides in marine sponges: Extraction methods and anti-HIV activity". Marine Drugs 9 1 (2011): 139-153. http://www.scopus.com/inward/record.url?eid=2-s2.0-79251517394&partnerID=MN8TOARS.
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  128. Eberhardy, S.R.; Goncalves, J.; Coelho, S.; Segal, D.J.; Berkhout, B.; Barbas III, C.F.. "Inhibition of human immunodeficiency virus type 1 replication with artificial transcription factors targeting the highly conserved primer-binding site". Journal of Virology 80 6 (2006): 2873-2883. http://www.scopus.com/inward/record.url?eid=2-s2.0-33644774584&partnerID=MN8TOARS.
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  129. Ribeiro, A.C.; Maia E Silva, A.; Santa-Marta, M.; Pombo, A.; Moniz-Pereira, J.; Goncalves, J.; Barahona, I.. "Functional analysis of Vif protein shows less restriction of human immunodeficiency virus type 2 by APOBEC3G". Journal of Virology 79 2 (2005): 823-833. http://www.scopus.com/inward/record.url?eid=2-s2.0-11144230447&partnerID=MN8TOARS.
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  130. Gonçalves, J.; Malta-Vacas, J.; Louis, M.; Brault, L.; Bagrel, D.; Monteiro, C.; Brito, M.. "Modulation of translation factor's gene expression by histone deacetylase inhibitors in breast cancer cells". Clinical Chemistry and Laboratory Medicine 43 2 (2005): 151-156. http://www.scopus.com/inward/record.url?eid=2-s2.0-14944357254&partnerID=MN8TOARS.
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  131. Corte-Real, S.; Collins, C.; Aires Da Silva, F.; Simas, J.P.; Barbas III, C.F.; Chang, Y.; Moore, P.; Goncalves, J.. "Intrabodies targeting the Kaposi sarcoma-associated herpesvirus latency antigen inhibit viral persistence in lymphoma cells". Blood 106 12 (2005): 3797-3802. http://www.scopus.com/inward/record.url?eid=2-s2.0-28444475543&partnerID=MN8TOARS.
    10.1182/blood-2005-04-1627
  132. Santa-Marta, M.; Da Silva, F.A.; Fonseca, A.M.; Goncalves, J.. "HIV-1 Vif can directly inhibit apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G-mediated cytidine deamination by using a single amino acid interaction and without protein degradation". Journal of Biological Chemistry 280 10 (2005): 8765-8775. http://www.scopus.com/inward/record.url?eid=2-s2.0-15744387175&partnerID=MN8TOARS.
    10.1074/jbc.M409309200
  133. Aires Da Silva, F.; Leite Costa, M.J.; Corte-Real, S.; Goncalves, J.. "Cell type-specific targeting with sindbis pseudotyped lentiviral vectors displaying anti-CCR5 single-chain antibodies". Human Gene Therapy 16 2 (2005): 223-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-14844317327&partnerID=MN8TOARS.
    10.1089/hum.2005.16.223
  134. Pereira, L.; Gonçalves, J.; Goios, A.; Rocha, T.; Amorim, A.. "Human mtDNA haplogroups and reduced male fertility: Real association or hidden population substructuring". International Journal of Andrology 28 4 (2005): 241-247. http://www.scopus.com/inward/record.url?eid=2-s2.0-23044507572&partnerID=MN8TOARS.
    10.1111/j.1365-2605.2005.00539.x
  135. Nolasco, S.; Bellido, J.; Gonçalves, J.; Zabala, J.C.; Soares, H.. "Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death". FEBS Letters 579 17 (2005): 3515-3524. http://www.scopus.com/inward/record.url?eid=2-s2.0-21244462806&partnerID=MN8TOARS.
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  136. Goncalves, J.; Santa-Marta, M.. "HIV-1 Vif and APOBEC3G: Multiple roads to one goal". Retrovirology 1 (2004): http://www.scopus.com/inward/record.url?eid=2-s2.0-18144376374&partnerID=MN8TOARS.
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  137. Segal, D.J.; Gonçalves, J.; Eberhardy, S.; Swan, C.H.; Torbett, B.E.; Li, X.; Barbas III, C.F.. "Attenuation of HIV-1 Replication in Primary Human Cells with a Designed Zinc Finger Transcription Factor". Journal of Biological Chemistry 279 15 (2004): 14509-14519. http://www.scopus.com/inward/record.url?eid=2-s2.0-2442536906&partnerID=MN8TOARS.
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  138. Goncalves, J.; Santa-Marta, M.. "HIV-1 Vif and APOBEC3G: multiple roads to one goal.". Retrovirology 1 (2004): http://www.scopus.com/inward/record.url?eid=2-s2.0-34248645132&partnerID=MN8TOARS.
  139. Da Silva, F.A.; Santa-Marta, M.; Freitas-Vieira, A.; Mascarenhas, P.; Barahona, I.; Moniz-Pereira, J.; Gabuzda, D.; Goncalves, J.. "Camelized rabbit-derived VH single-domain intrabodies against Vif strongly neutralize HIV-1 infectivity". Journal of Molecular Biology 340 3 (2004): 525-542. http://www.scopus.com/inward/record.url?eid=2-s2.0-2942724405&partnerID=MN8TOARS.
    10.1016/j.jmb.2004.04.062
  140. Mehle, A.; Goncalves, J.; Santa-Marta, M.; McPike, M.; Gabuzda, D.. "Phosphorylation of a novel SOCS-box regulates assembly of the HIV-1 Vif-Cul5 complex that promotes APOBEC3G degradation". Genes and Development 18 23 (2004): 2861-2866. http://www.scopus.com/inward/record.url?eid=2-s2.0-10044230343&partnerID=MN8TOARS.
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Preprint
  1. "Origin, Phylogeny, Variability and Epitope Conservation of SARS-CoV-2 Worldwide". 2021. http://dx.doi.org/10.20944/preprints202105.0750.v1.
    10.20944/preprints202105.0750.v1

Other

Other output
  1. Transcriptome-wide off-target effects of steric-blocking oligonucleotides. 2020. Holgersen, E.M.; Gandhi, S.; Zhou, Y.; Kim, J.; Vaz, B.; Bogojeski, J.; Bugno, M.; et al. http://www.scopus.com/inward/record.url?eid=2-s2.0-85098907384&partnerID=MN8TOARS.
    10.1101/2020.09.03.281667
  2. LUZP1 and the tumour suppressor EPLIN are negative regulators of primary cilia formation. 2019. Gonçalves, J.; Coyaud, É.; Laurent, E.M.N.; Raught, B.; Pelletier, L.. http://www.scopus.com/inward/record.url?eid=2-s2.0-85095489184&partnerID=MN8TOARS.
    10.1101/736389
Activities

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2022 - Current COVID-19 vaccines in patients with chronic liver diseases: immunological responses and regulation by microRNAs
Co-supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2021 - Current The impact of TCF1 in T-cell memory self-renewal and the effectiveness of armored CAR-T in solid tumors
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2020 - Current Novel Strategy to Detect and Localize Latent HIV Reservoirs
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2019/01/01 - Current Engineering cell lines capable to produce artificial libraries of fully human antibodies
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2018/05/01 - Current Optimize anti-nucleolin antibodies with potent ADCC activity
Co-supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2018/04/01 - Current Development of a microfluidic system to assess the immunogenicity against therapeutic antibodies
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2018/03/01 - Current Cell microfluidic systems to discover antibodies by phage libraries
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2018 - Current HIV-1 Vif as a theraputic target for AIDS
Co-supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2021 - 2025 CAR (chimeric antigen receptor)-T cells secreting anti-PD-L1 antibodies: combining nucleolin-mediated checkpoint blockade and innate antitumor immunity against Triple-Negative Breast Cancer
Co-supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2021 - 2022 Development of Syn-Notch activators for CAR-T
Supervisor
Mestrado Ciencias Biofarmaceuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2019 - 2020 Engineering biosensor cells to detect cancer biomarkers
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2017 - 2020 Tumor targeting and therapeutic activity of nanobody-based platforms against breast cancer and angiogenic endothelial cells
Co-supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2015/03/01 - 2019/05/30 CellectAb as a novel process of generating therapeutic antibodies
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2018 - 2019 Mammary organoids to study the functional impact of BRCA mutations
Supervisor
Mestrado Ciencias Biofarmaceuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2017 - 2018 Engineering and optimizing pro-immunotoxins against HER2 positive cells
Supervisor
mestrado Ciencias biofarmaceuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2017 - 2018 Development of CRISP-induced variability in antibody CDR for the discovery of fully human antibodies with therapeutic activities
Supervisor
Mestrado em Ciências Biofarmacêuticas (PhD)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2016 - 2017 Eradication of HIV by zinc-finger nucleases
Supervisor
Mestrado em Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2013 - 2017 Eradication of HIV-1 infected cells by targeting nanoparticles carrying therapeutic plasmids
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2015 - 2016 New concept of ADC by dimeric antibodies
Supervisor
Mestrado em Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2015 - 2016 Antibody-ZincFinger conjugates as new immunotherapy strategy
Supervisor
Mestrado em Ciências Biofarmacêuticas
Universidade de Lisboa Faculdade de Farmácia, Portugal
2012 - 2016 Engineering synthetic domain antibodies with broad and potent HIV-1 neutralization
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2015 - 2015 CAR-T selection of antibodies
Supervisor
Mestrado em Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2014 - 2015 Targeting Membrane Proteins of Latently Infected CD4+ T Cells Providing a Starting Point to the Eradication of HIV-1
Supervisor
Mestrado em Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2010 - 2014 Novel HIV-1 Knockdown Targets Identified by an Enriched Kinases/Phosphatases shRNA Library Using a Long-Term Iterative Screen in Jurkat T-Cells
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2009 - 2013 Innovative technologies to integrate in vitro screening and optimization of recombinant therapeutic antibodies
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2009 - 2013 Engineering new strategies to improve pharmacokinetics of therapeutic proteins
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2009 - 2013 Lentiviral target-specific strategy for molecular therapy
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2009 - 2013 Functional aspects of new helper factors for HIV replication
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2008 - 2012 Measles vírus: early infection, progression and pathogenesis in a transgenic mouse model
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2010 - 2010 Specific elimination of HIV-1 infected cells using a Tat/Ver-dependent circuit
Supervisor
mestrado ciencias biofarmaceuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2009 - 2010 Selecção de pequenos domínios de anticorpos específicos para o péptido ß-amilóide
Supervisor
mestrado ciencias biofarmaceuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2009 - 2010 Factores de transcrição artificiais na identificação de genes celulares importantes na infecciosidade do HIV-1
Supervisor
mestrado ciencias biofarmaceuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2005 - 2009 Development of intrabodies for neutralization of HIV-1 integrase
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2005 - 2009 Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal
2005 - 2009 Intrabodies targeting the Kaposi sarcoma–associated herpesvirus latency antigen inhibit viral persistence in lymphoma cells
Supervisor
Universidade de Lisboa Faculdade de Farmácia, Portugal

Committee member

Activity description
Role
Institution / Organization
2021 - Current Comissão de Avaliação de Medicamentos - Infarmed
Member
Infarmed Autoridade Nacional do Medicamento e Produtos de Saúde IP Centro de Documentação Técnica e Científica, Portugal

Consulting

Activity description Institution / Organization
2019 - 2022 Membro do laboratório colaborativo VectorB2B - Administração VectorB2B, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2015 - 2022 I&D biofarmacos Doutoramento (Doutoramento) Universidade de Lisboa Faculdade de Farmácia, Portugal
2012 - 2022 I&D biofarmacos Mestrado de ciencias biofarmaceuticas (Mestrado) Universidade de Lisboa Faculdade de Farmácia, Portugal
2008 - 2022 Imunologia Ciências Farmacêuticas (Mestrado integrado) Universidade de Lisboa Faculdade de Farmácia, Portugal
2008 - 2022 Biotecnologia Farmacêutica Ciencias Farmaceuticas (Mestrado integrado) Universidade de Lisboa Faculdade de Farmácia, Portugal
Distinctions

Award

2015 Premio Genese
Gilead Sciences, France
2010 Premio HIV/SIDA
Direcção-Geral da Saúde, Portugal
2006 Premio HIV/SIDA
Direcção-Geral da Saúde, Portugal