Marisa Nicolai has a degree in Chemistry (2001), a master’s degree in teaching of Physics and Chemistry (2012) and PhD in Chemistry (2009). She is an Assistant Professor at Universidade Lusófona. Since 2001 on, she has published several papers in peer-reviewed international journals, book chapters, and dissemination publications. Marisa Nicolai reviewed more than fifty papers published in international journals. She is currently the guest editor of a special issue. and author/co-author of more than one hundred oral communications and posters in international and national meetings. She has received three awards. Currently she is supervising a PhD student, several master dissertations and participated in several academic juries. M. Nicolai is a member of the Scientific Council of the School of Health Sciences and Technologies of Universidade Lusófona, since 2015, and Member of the Pedagogical Council of Universidade Lusófona since 2019. She has integrated the Organizing Committee of some international and national meetings and participates frequently in public outreach activities. She is presently interested in research involving health sciences and human nutrition, where she applies her skills in analytical chemistry, embracing her expertise in chromatographic techniques, mass spectrometry, cell culture, including cytotoxicity assays. These researches may impact the development of nutritional and therapeutic approaches.

Personal identification

Full name
Marisa Helena Fonseca Nicolai

Citation names

  • Nicolai, Marisa

Author identifiers

Ciência ID
Researcher Id
Scopus Author Id

Email addresses

  • marisa.nicolai@ulusofona.pt (Professional)

Knowledge fields

  • Exact Sciences - Chemical Sciences
Degree Classification
2010/09 - 2012/11
Mestrado em Ensino de Física e de Química (Mestrado)
Major in Ensino de Física e de Química
Universidade de Lisboa Faculdade de Ciências, Portugal
17 valores
2003/01 - 2008/12
Química (Doutoramento)
Major in Química Inorgânica
Universidade de Lisboa Faculdade de Ciências, Portugal
Distinção e Louvor
1996/11 - 2001/10
Química (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
16 valores

Teaching in Higher Education

Host institution
2013 - Current Assistant Professor (University Teacher) Universidade Lusófona, Portugal
Universidade Lusófona de Humanidades e Tecnologias Centro de Investigação em Biociências e Tecnologias da Saúde, Portugal
2015 - 2016 Teacher Coordinator (Polytechnic Teacher) Escola Superior de Saúde Ribeiro Sanches, Portugal
2009/09/01 - 2012/08/31 Invited Assistant Professor (University Teacher) Universidade de Lisboa Faculdade de Medicina Dentária, Portugal
2008/09/01 - 2009/08/31 Invited Assistant (University Teacher) Universidade de Lisboa Faculdade de Medicina Dentária, Portugal

Positions / Appointments

Host institution
2016/03 - Current Membro do Conselho Científico ECTS Universidade Lusófona de Humanidades e Tecnologias Centro de Investigação em Biociências e Tecnologias da Saúde, Portugal
2015/09 - Current Membro Integrado CBIOS Universidade Lusófona de Humanidades e Tecnologias Centro de Investigação em Biociências e Tecnologias da Saúde, Portugal
2015 - 2016 Conselho científico/técnico-científico ou orgão correspondente Escola Superior de Saúde Ribeiro Sanches, Portugal
2015 - 2016 Conselho pedagógico Escola Superior de Saúde Ribeiro Sanches, Portugal


Host institution
2019 - Current Membro Conselho Pedagógico ULHT Universidade Lusófona, Portugal
2009/04 - 2012/08 Integrated Member of UICOB Universidade de Lisboa Faculdade de Medicina Dentária, Portugal
2003/01/02 - 2008/12/30 PhD student Universidade de Lisboa Faculdade de Ciências, Portugal


Book chapter
  1. Nicolai, Marisa; Pereira, Paula Cristina da Silva França Gomes ; Palma, Maria Lídia Laginha Mestre Guerreiro da. Corresponding author: Palma, Maria Lídia Laginha Mestre Guerreiro da. "The Influence of Some Contaminants in Food Quality". In Mycotoxins and Food Safety. London, United Kingdom: IntechOpen, 2022.
Journal article
  1. Maria Lidia Palma; Mariana Batalha; Joana Pereira; Maria Joao Cebola; Paula Pereira; Marisa Nicolai. "Food Frequency Questionnaire to Assess Fiber Intake and Its Relationship with Health". International Journal of Nutrition and Food Sciences (2023): http://dx.doi.org/10.11648/j.ijnfs.20231203.12.
  2. Joana Marcos; Raquel Carriço; Maria João Sousa; M. Lídia Palma; Paula Pereira; M. Cristiana Nunes; Marisa Nicolai. "Effect of Grape Pomace Flour in Savory Crackers: Technological, Nutritional and Sensory Properties". Foods (2023): https://doi.org/10.3390/foods12071392.
  3. Joana Marcos; Duarte Gomes; M. Lídia Palma; M. João Cebola; Paula Pereira; Marisa Nicolai; Raquel Carriço. "Influence of Front-of-Pack Nutrition Labelling on Portuguese Consumers’ Food Preferences - a Preliminary Study". Research Journal of Food and Nutrition (2023): http://dx.doi.org/10.22259/2637-5583.0601002.
  4. Ana Morais; Patrícia Rijo; Belen Batanero; Marisa Nicolai. "Low Platinum-Content Electrocatalysts for Highly Sensitive Detection of Endogenously Released H2O2". Biosensors (2022): https://doi.org/10.3390/bios12090672.
  5. Ana Morais; Belen Batanero; Patrícia Rijo; Marisa Nicolai. "Ionic exchange membranes in the pharmaceutical industry – Review". Journal Biomedical and Biopharmaceutical Research (2022): https://doi.org/10.19277/bbr.19.1.285.
  6. Ana Júlio; Nádia Remtula; Marisa Nicolai; Tânia Santos de Almeida. "Ionic liquids as tools to improve gel formulations containing sparingly soluble phenolic acids". Journal Biomedical and Biopharmaceutical Research (2022): https://doi.org/10.19277/bbr.19.1.280.
  7. Nicolai, Marisa. "Impact of Portuguese propolis on keratinocyte proliferation, migration and ROS protection: Significance for applications in skin products". International Journal of Cosmetic Science (2022): http://dx.doi.org/10.1111/ics.12781.
  8. Raphaela Cassol Piccoli; Paula Pereira; Marisa Nicolai; Francieli Moro Stefanello; Rejane Tavares Giacomelli; Maria Lídia Palma. "Grape Pomace Flour: from winemaking by-product to sustainable alternative for health benefits". Journal Biomedical and Biopharmaceutical Research (2022): https://doi.org/10.19277/bbr.19.2.296.
  9. Patrícia Rijo; Lara Ribeiro; Marisa Nicolai; Paula Pereira; Célia Faustino. "Rapid UV-Vis spectroscopy methods for quantification of ranitidine tablets". Journal Biomedical and Biopharmaceutical Research (2021): https://doi.org/10.19277/bbr.18.2.271.
  10. M. Lídia Palma; Cíntia Ferreira Pêgo; Marisa Nicolai; Paula Pereira. "Preliminary sensory evaluation of grpe pomace flour sweet cookies". Biomedical and Biopharmaceutical Research Journal 18 1 (2021): 1-11. https://doi.org/10.19277/bbr.18.1.249.
  11. Joana M. Andrade; Eva María Domínguez-Martín; Marisa Nicolai; Célia Faustino; Luís Monteiro Rodrigues; Patrícia Rijo; J Marçalo; et al. "Screening the dermatological potential of Plectranthus species components: antioxidant and inhibitory capacities over elastase, collagenase and tyrosinase". Journal of Enzyme Inhibition and Medicinal Chemistry 36 1 (2021): 257-269. https://doi.org/10.1080/14756366.2020.1862099.
  12. Paula Pereira; Carla Palma; Cíntia Ferreira-Pêgo; Olga Amaral; Anabela Amaral; Patrícia Rijo; João Gregório; Lídia Palma; Marisa Nicolai. "Grape Pomace: A Potential Ingredient for the Human Diet". Foods 9 12 (2020): 1772-1772. https://doi.org/10.3390/foods9121772.
  13. Ana Lúcia Morais; Patrícia Rijo; Belén Batanero; Marisa Nicolai. "Development of New Catalytic Material for Accurate Detection of Biological Biomarkers Related to Most Common Non-Communicable Diseases". Proceedings 60 1 (2020): 34-34. https://doi.org/10.3390/IECB2020-07090.
  14. Ana Lúcia Morais; Patrícia Rijo; María Belén Batanero Hernán; Marisa Nicolai. "Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review". Biosensors 10 9 (2020): 121-121. https://doi.org/10.3390/bios10090121.
  15. Marisa Nicolai; Joana Mota; Ana S. Fernandes; Filipe Pereira; Paula Pereira; Catarina P. Reis; Maria Valéria Robles Velasco; et al. "Assessment of the Potential Skin Application of Plectranthus ecklonii Benth.". Pharmaceuticals 13 6 (2020): 120-120. https://doi.org/10.3390/ph13060120.
  16. Palma, M.L.; Nunes, M.C.; Gameiro, R.; Rodrigues, M.; Gothe, S.; Tavares, N.; Pego, C.; Nicolai, M.; Pereira, P.. "Preliminary sensory evaluation of salty crackers with grape pomace flour,Avaliação sensorial preliminar de bolachas salgadas de farinha de bagaço de uva". Biomedical and Biopharmaceutical Research 17 1 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85085128368&partnerID=MN8TOARS.
  17. Motta, C.; Matos, A.S.; Soares, A.; Gonzales, G.B.; Castanheira, I.; Cabral, I.; Tavares, N.; et al. "Amino acid profile of foods from the Portuguese Total Diet Pilot Study". Journal of Food Composition and Analysis 92 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85086116346&partnerID=MN8TOARS.
  18. Matias, D.; Nicolai, M.; Fernandes, A.S.; Saraiva, N.; Almeida, J.; Saraiva, L.; Faustino, C.; et al. "Comparison Study of Different Extracts of Plectranthus madagascariensis, P. neochilus and the Rare P. porcatus (Lamiaceae): Chemical Characterization, Antioxidant, Antimicrobial and Cytotoxic Activities". Biomolecules 9 5 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85065872527&partnerID=MN8TOARS.
  19. Matias, D.; Nicolai, M.; Saraiva, L.; Pinheiro, R.; Faustino, C.; Diaz Lanza, A.; Pinto Reis, C.; et al. "Cytotoxic Activity of Royleanone Diterpenes from Plectranthus madagascariensis Benth". ACS Omega 4 5 (2019): 8094-8103. http://www.scopus.com/inward/record.url?eid=2-s2.0-85065225229&partnerID=MN8TOARS.
  20. Nicolai, M.; Almeida, N.; Rijo, P.; Costa, J.G.; Saraiva, N.; Fernandes, A.S.. "Cytotoxic effect of antioxidants found in food from plant origin on human osteosarcoma U2OS cells,Efeito citotóxico de antioxidantes de alimentos de origem vegetal em células de osteossarcoma humano U2OS". Biomedical and Biopharmaceutical Research 16 1 (2019): 86-96. http://www.scopus.com/inward/record.url?eid=2-s2.0-85075688252&partnerID=MN8TOARS.
  21. Marisa Nicolai; Paula Pereira; Patricia Rijo; Olga Amaral; Anabela Amaral; Lídia Palma. "Vitis vinera L. pomace: chemical and nutritional characterization". Journal Biomedical and Biopharmaceutical Research (2018): https://doi.org/10.19277/BBR.15.2.182.
  22. Garcia, Catarina; Oliveira Silva, Catarina; Monteiro, Carlos M.; Nicolai, Marisa; Viana, Ana; Andrade, Joana M.; Barasoain, Isabel; et al. "Anticancer properties of the abietane diterpene 6,7-dehydroroyleanone obtained by optimized extraction". Future Medicinal Chemistry (2018): https://publons.com/publon/1596458/.
  23. Coelho, Jose; Veiga, Jerson; Karmali, Amin; Nicolai, Marisa; Reis, Catarina Pinto; Nobre, Beatriz; Palavra, Antonio; et al. "Supercritical CO2 Extracts and Volatile Oil of Basil (Ocimum basilicum L.) Comparison with Conventional Methods". Separations (2018): https://publons.com/publon/5815021/.
  24. Siopa, Filipa; Frade, Raquel F. M.; Diniz, Ana; Andrade, Joana M.; Nicolai, Marisa; Meirinhos, Ana; Lucas, Susana D.; et al. "Acetylcholinesterase Choline-Based Ionic Liquid Inhibitors: In Vitro and in Silico Molecular Docking Studies". ACS Omega 3 12 (2018): 17145-17154. http://hdl.handle.net/10362/104680.
  25. Ana Lucía Morocho-Jácome; Marcello Dapievi Bresaola; João Carlos Monteiro de Carvalho; Marisa Nicolai; Catarina Rosado; André Rolim Baby. "Carbohydrates in Ankistrodesmus braunii biomass cultivated in tubular photobioreactors". Journal Biomedical and Biopharmaceutical Research (2017): https://doi.org/10.19277/bbr.14.2.166.
  26. Mota, A.H.; Silva, C.O.; Nicolai, M.; Baby, A.; Palma, L.; Rijo, P.; Ascensão, L.; Reis, C.P.. "Design and evaluation of novel topical formulation with olive oil as natural functional active". Pharmaceutical Development and Technology (2017): 1-12. http://www.scopus.com/inward/record.url?eid=2-s2.0-85021845504&partnerID=MN8TOARS.
  27. Ana Silva; João Moreira; Pedro Andrade; Marisa Nicolai; Patricia Rijo; Nelson Tavares. "Production of fermented Thai red glutinous rice using an isolated Monascus purpureus NART001 from commercially available Chinese red fermented rice". Journal Biomedical and Biopharmaceutical Research (2016): https://doi.org/10.19277/bbr.13.2.138.
  28. Marisa Nicolai; Paula Pereira; Rute F. Vitor; Catarina Pinto Reis; Amilcar Roberto; Patrícia Rijo. "Antioxidant activity and rosmarinic acid content of ultrasound-assisted ethanolic extracts of medicinal plants". Measurement 89 (2016): 328-332. https://doi.org/10.1016%2Fj.measurement.2016.04.033.
  29. Wagemaker, T.A.L.; Campos, P.M.B.G.M.; Fernandes, A.S.; Rijo, P.; Nicolai, M.; Roberto, A.; Rosado, C.; et al. "Unsaponifiable matter from oil of green coffee beans: cosmetic properties and safety evaluation". Drug Development and Industrial Pharmacy 42 10 (2016): 1695-1699. http://www.scopus.com/inward/record.url?eid=2-s2.0-84965010204&partnerID=MN8TOARS.
  30. D. Matias; M. Nicolai; J. Costa; N. Saraiva; A.S. Fernandes; M.F. Simões; A.M. Diaz-Lanza; et al. "Cytotoxicity screening of Plectranthus spp. extracts and individual components in MDA-MB-231 cells". Toxicology Letters 238 2 (2015): S240-S240. https://doi.org/10.1016%2Fj.toxlet.2015.08.707.
  31. De Aguiar, S.R.M.M.; Nicolai, M.; Almeida, M.; Gomes, A.. "Electrochemical behaviour of a cobalt-chromium-molybdenum dental alloy in artificial salivas". Bio-Medical Materials and Engineering 25 1 (2015): 53-66. http://www.scopus.com/inward/record.url?eid=2-s2.0-84921038379&partnerID=MN8TOARS.
  32. D Matias; F Pereira; M Nicolai; A Roberto; N Saraiva; AS Fernandes; MF Simões; et al. "Abietane diterpenes from Plectranthus madagascariensis: A cytotoxicity screening". Planta Medica 80 16 (2014): https://doi.org/10.1055%2Fs-0034-1394809.
  33. Carolina Correia; Patricia Rijo; Lia Ascensão; Marisa Nicolai; Diogo Matias; Catarina Pinto Reis. "Optimization of the encapsulation efficiency of a novel oral insulin delivery nanosystem". Journal Biomedical and Biopharmaceutical Research (2014): https://doi.org/10.19277/BBR.11.1.82.
  34. Pedro Faisca; Joana Portugal Mota; Maria João Soares Rodrigues de Sousa Fernandes; Marisa Nicolai. "Supplement - CBiOS Science Sessions - 2014". Journal Biomedical and Biopharmaceutical Research (2014): https://doi.org/10.19277/BBR.11.1.84.
  35. Rijo, P.; Matias, D.; Fernandes, A.S.; Simões, M.F.; Nicolai, M.; Reis, C.P.. "Antimicrobial plant extracts encapsulated into polymeric beads for potential application on the skin". Polymers 6 2 (2014): 479-490. http://www.scopus.com/inward/record.url?eid=2-s2.0-84899496961&partnerID=MN8TOARS.
  36. Marisa Nicolai; Vanessa Amaral; Cátia Antunes; Duangratana Shuwisitkul; Joana Portugal Mota. "Lipid-Based Implants: Impact of Formulation Parameters". Advanced Materials Research 1060 (2014): 87-90. https://doi.org/10.4028%2Fwww.scientific.net%2Famr.1060.87.
  37. Joana Portugal Mota; Alexandre Campos; Duangratana Shuwisitkul; Nuno Saraiva; Marisa Nicolai. "Lysozyme Release from Lipid-Based Implants". Advanced Materials Research 1060 (2014): 83-86. https://doi.org/10.4028%2Fwww.scientific.net%2Famr.1060.83.
  38. Marisa Nicolai; Gisela Gonçalves; Filipe Natalio; Madalena Humanes; Nicolai, M.; Gonçalves, G.; Natalio, F.; Humanes, M.. "Biocatalytic formation of synthetic melanin: The role of vanadium haloperoxidases, L-DOPA and iodide". Journal of Inorganic Biochemistry 105 6 (2011): 887-893. https://publons.com/publon/11625074/.
  39. Esteves, Ana I. S.; Nicolai, Marisa; Humanes, Madalena; Gonçalves, João; Esteves, A.I.S.; Nicolai, M.; Humanes, M.; et al. "Sulfated polysaccharides in marine sponges: extraction methods and anti-HIV activity". Marine Drugs 9 1 (2011): 139-153. http://hdl.handle.net/10400.1/11384.
Thesis / Dissertation
  1. Nunes, Miguel Alexandre Martins Garcia. "O diagnóstico na diabetes : estudo numa farmácia comunitária". Master, 2020. http://hdl.handle.net/10437/11834.
  2. Ladeiras, Vera Maria Fernandes Bugalho. "Análises sanguíneas na detecção de cancro". Master, 2019. http://hdl.handle.net/10437/10128.
  3. Carvalho, Ana Rita Pires de. "Estudo da libertação de lisozima usando implantes lipídicos como sistemas de veiculação". Master, 2016. http://hdl.handle.net/10437/7478.
  4. Silva, Ana Margarida Pereira da. "Infeções e utilização de antibióticos na ortopedia". Master, 2015. http://hdl.handle.net/10437/6353.
  5. Teixeira, Liliana Sofia Martins. "Contribuição do tratamento com fármacos antiepiléticos para o declínio cognitivo na epilepsia". Master, 2015. http://hdl.handle.net/10437/6352.
  6. Lopes, Ana Alexandra Santos; Matos, Mónica José Ciríaco Pinheiro Pombinho. "Inibidores de tirosinase e novas técnicas laboratoriais de separação de produtos naturais bioactivos". Master, 2014. http://hdl.handle.net/10437/6681.
  7. Nicolai, Marisa Helena Fonseca, 1976-. "Tarefas de investigação com recurso aos wikis no ensino da tabela periódica dos elementos : um estudo com alunos do 9.º ano de escolaridade". Master, 2012. http://hdl.handle.net/10451/11023.
  8. Nicolai, Marisa; Nicolai, Marisa Helena Fonseca, 1976-. "Caracterização de macromoleculas biológicas de organismos marinhos". PhD, 2009. http://sibul.reitoria.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000551125.

Oral presentation

Presentation title Event name
Host (Event location)
2019/07/08 Antimicrobial, cytotoxic and antioxidant properties of lead molecules from Plectranthus spp. Ciência 2019
(Lisboa, Portugal)
2019/05 Effect of antioxidants from vegetables and fruits on Osteosarcoma Cells Dia Internacional do Fascinio das Plantas
CBIOS (Lisboa, Portugal)
2019/02/19 Antitubercular activity and mitochondrial dysfunction of halimane and labdane diterpenoids from Plectranthus ornatus Codd. Meeting dedicated to ECIs
COST Action CM1407 (Bruxelas, Belgium)
2018/10 Effect of antioxidants from vegetables and fruits on Human Osteosarcoma U2-OS Cells II Jornadas da Nutrição
CBIOS (Lisboa, Portugal)
2018/10 Vitis vinera L. pomace: chemical and nutritional characterization II Jornadas da Nutrição
CBIOS (Lisboa, Portugal)
2017/12 Halimane diterpenes from Plectranthus ornatus Codd. against Mycobacterium tuberculosis H37Rv UL Chemistry PhD Meeting (2ECQUL)
Colégio de Química, Universidade de Lisboa (Lisboa, Portugal)