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Maria C. Pedroso de Lima (h-index 51, Scopus) is Full Professor at the University of Coimbra, Portugal. She received her Ph.D. degree in Chemistry from Imperial College London, UK, and her Habilitation (Agregação) degree in Biochemistry from the University of Coimbra, Portugal. She was Head of the Group "Vectors and Gene Therapy" at the CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Portugal. The research team provided a significant and important contribution to the design and development of nucleic acid/drug carriers, including viral and nonviral vectors, which have been successfully applied in 1) establishment of disease models; 2) study of disease mechanisms; and 3) generation of new molecular therapeutic approaches for cancer and neurodegenerative disorders and of prophylactic strategies. More recently, as Leader of the Group "Therapies Targeting Brain Diseases: Genomic and Lipidomic Approaches" at the CNC, her central mission has been to generate innovative and effective gene and lipid therapies for brain cancer, namely glioblastoma (GBM), employing novel functionalized delivery nanosystems for tumor-specific targeting, towards clinical translation. The research work of the team has been mainly focused on 1) evaluation of the therapeutic efficacy of the developed targeted therapeutic approaches in cell and animal models of GBM; 2) investigation of the molecular mechanisms underlying the process of nucleic acid delivery and the molecular/cellular events associated to the therapeutic response; and 3) study of the role of GBM stem cells (GSCs) in tumor progression and identification of new molecular targets involved in the renewal and differentiation of GSCs. She published over 200 papers in international peer-reviewed journals and 33 international scientific book chapters, with over 8,440 citations (Scopus). She has authored over 300 communications presented in scientific meetings. She is co-inventor of 5 patents on non-viral drug/gene delivery and gene therapy, 4 of which approved in USA. She has edited/co-edited 2 books, one (Editor) published by Springer-Verlag and the other (Co-Editor) published by Translational Research Network. She is a reviewer for over 25 international scientific journals. She was Chair and member of the Scientific Committee of national and international conferences in the fields of membrane fusion and gene therapy and was the Director of a NATO Advanced Study Institute on the trafficking of intracellular membranes and membrane fusion. She has successfully supervised 4 post-doctoral researchers, 24 PhD students and over 30 Master students. She received the international 2013 Exiqon award for a grant proposal on the development and application of a targeted nanosystem for systemic delivery in a miRNA-based therapy towards glioblastoma. She is listed as one of the top 2% of cited scientists in 2021 and throughout her career (compiled lists by the Stanford University). She has served as evaluator of research proposals, both for the European Union and national governments (United Kingdom, Israel, Poland and Portugal). She has been responsible for national and international research projects funded by the NATO Scientific Affairs Division, European Union, CRUP and the Portuguese Foundation for Science and Technology (FCT).
Identification

Personal identification

Full name
Maria da Conceição Monteiro Pedroso de Lima

Citation names

  • Lima, Maria
  • Maria C. Pedroso de Lima
  • Maria Pedroso de Lima

Author identifiers

Ciência ID
A11E-4930-EEEA
ORCID iD
0000-0003-1844-5027
Scopus Author Id
7004364781

Email addresses

  • mdelima@ci.uc.pt (Professional)
  • mdelima@cnc.uc.pt (Professional)

Telephones

Telephone
  • 239820190 (Professional)

Addresses

  • Universidade de Coimbra . Centro de Neurociências e Biologia Celular, Largo Marquês de Pombal, Universidade de Coimbra. Coimbra, 3004-517, Coimbra, Coimbra, Portugal (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
  • Natural sciences - Biological Sciences - Cell Biology
  • Engineering and Technology - Nano-technology
  • Natural sciences - Biological Sciences - Biochemistry
  • Medical and Health Sciences - Basic Medicine - Medicinal Chemistry

Languages

Language Speaking Reading Writing Listening Peer-review
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
Education
Degree Classification
1997
Concluded
Biochemistry (Título de Agregado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"A hemaglutinina do vírus Influenza: Uma proteína modelo no estudo dos mecanismos de fusão membranar " (THESIS/DISSERTATION)
Aprovação por unanimidade
1977/03
Concluded
Chemistry (Doctor of Philosophy)
Major in Chemistry
Imperial College of Science and Technology, University of London , United Kingdom
"Transference number studies by the direct moving boundary method" (THESIS/DISSERTATION)
Approved with greatest honors
1972
Concluded
Química (Licenciatura)
Universidade do Porto Faculdade de Ciências, Portugal
"." (THESIS/DISSERTATION)
Distinção
Affiliation

Science

Category
Host institution
Employer
2003 - Current Principal Investigator (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Teaching in Higher Education

Category
Host institution
Employer
1998 - Current Full Professor (University Teacher) Universidade de Coimbra, Faculdade de Ciências , Portugal
1981 - 1998 Associate Professor (University Teacher) Universidade de Coimbra, Faculdade de Ciências , Portugal
1988 - 1990 Visiting Professor (University Teacher) University of California San Francisco Medical Center at Parnassus, United States
University of California San Francisco Medical Center at Parnassus, United States
1983 - 1984 Visiting Professor (University Teacher) University of California Berkeley Department of Chemistry, United States
University of California Berkeley, United States
1977 - 1981 Assistant Professor (University Teacher) Universidade de Coimbra, Faculdade de Ciências , Portugal
1973 - 1977 Assistant (University Teacher) Universidade do Porto Faculdade de Ciências, Portugal
Universidade do Porto Faculdade de Ciências, Portugal

Positions / Appointments

Category
Host institution
Employer
2006 - 2008 Organic Unit President Department of Biochemistry, Faculty of Science and Technology, University of Coimbra, Portugal
Department of Biochemistry, Faculty of Science and Technology, University of Coimbra, Portugal
1997 - 1999 Organic Unit President Department of Biochemistry, Faculty of Science and Technology, University of Coimbra, Portugal
Department of Biochemistry, Faculty of Science and Technology, University of Coimbra, Portugal
Projects

Grant

Designation Funders
2017 - 2021 Tackling Cancer Stem Cells: A Challenge and an Opportunity to Advance in Anti-Cancer Therapy
FCT-POCI-01-0145-FEDER-016390
Researcher
Fundação para a Ciência e a Tecnologia
2018 - 2020 Thioredoxin Arrest to Reduce Glioblastoma: Ethylmercury and Thimerosal to target CANCER
C490709752-00081714
Researcher
Universidade de Lisboa Faculdade de Farmácia, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2017 - 2020 BrainHealth2020- Early Detection, Neuromodulation and Advanced Therapies to Brain Disorders
CENTRO-01-0145-FEDER-000008
Researcher
Comissão de Coordenação e Desenvolvimento Regional do Centro
Ongoing
2016/01 - 2018/12 A Lipidomic and MiRNA-Based Strategy for GlioblastomaTtreatment
A03/2016
Principal investigator
CRUP/DAAD
Concluded
2013/03 - 2015/08 Exploring miRNA contribution to amyloid-b-metabolism: development of a novel lentiviral platform for multiple miRNA expression in Alzheimer´s disease
Researcher
Fundação para a Ciência e a Tecnologia
2013/03 - 2015/03 Combined therapeutic approach based on miRNA modulation towards glioblastoma multiforme: A novel targeted lipid-based nanosystem for systemic delivery
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
2010/02 - 2012/02 Suicide gene therapy in cancer stem cells from solid tumors: towards a new antitumoral strategy
No reference code Proc 441.00
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2009/07 - 2011/12 Advancing the field of drug delivery - combined targeted treatments against human breast cancer and human leukemia (The OncotargetNanoMed network)
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2009 - 2011 Interaction of Lipoplexes with Cellular Membranes: a Biophysical Approach to Gene Delivery
PTDC/QUI-BIQ/103001/2008
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2007/07 - 2010/12 Silencing Machado-Joseph disease: lentiviral-mediated RNA interference to ataxin-3
Researcher
Fundação para a Ciência e a Tecnologia
2007/05 - 2010/12 Development of novel approaches for anti-tumor therapy based on the S4(13)-PV cell penetrating peptide to improve the intracellular delivery of therapeutic nucleic acids and proteins
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
2005/09 - 2008/08 Lentiviral vectors for gene silencing in polyglutamine disorders: Ataxin-3 gene silencing by lentiviral vectors-mediated delivery of double-stranded RNAs
Researcher
Fundação para a Ciência e a Tecnologia
2005/09 - 2008/02 Targeted drug delivery to tumor vasculature: a new therapy against human breast cancer.
Researcher
Fundação para a Ciência e a Tecnologia
2004/12 - 2007/11 Mechanisms of nucleo-cytoplasmic trafficking of ASFV genome and design of anti-NLS/NES backbone cyclic peptides to block viral infection
Principal investigator
Fundação para a Ciência e a Tecnologia
2003 - 2006 Targeted antisense therapy: a novel approach for the treatment of human small cell lung cancer
POCTI/FCB/48487/2002
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2003 - 2006 Targeted Contrast Agents for Magnetic Resonance Imaging (MRI) and Nuclear Scintigraphy
(TARCAS) POCTI/QUI/47005/2002
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2003/01 - 2005/12 Mechanisms of nucleo-cytoplasmic trafficking of ASFV genome and design of anti-NLS/NES backbone cyclic peptides to prevent viral infection
Provided by PTCRIS: 42700
Principal investigator
Fundação para a Ciência e a Tecnologia
2001 - 2004 Targeting of genes and antisense oligonucleotides by novel liposome formulations: evaluation of their therapeutic efficacy in human leukaemia.
FCT/BIO/36202/99
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2000 - 2002 Strategies to Enhance Gene Delivery: Overcoming the Nuclear Barrier
No reference code
Principal investigator
NATO Scientific Affairs Division
Concluded
1997/09 - 2000/09 Non-Viral Gene Transfer Vectors.
EC BIO4-CT97-2191
Principal investigator
European Commission
Concluded
1997 - 2000 Transport and delivery of genes and oligonucleotides mediated by liposomes: Mechanisms of internalization and design of novel formulations
PRAXIS/ PCNA/P/BIO/45/ 96
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
1993 - 1998 Mechanisms of genetic variability and immunoresistance to African Swine Fever Virus
PBIC/P/AGR/1824/93
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
1993 - 1996 Mechanisms of fusion and uncoating of African Swine Fever virus: Initial stages of viral infection
STRDA/C/AGR/191/92
Principal investigator
Fundação para a Ciência e a Tecnologia
1990 - 1994 Fusion Activity of lipid enveloped viruses
CRG.900333
Principal investigator
NATO Scientific Affairs Division
Concluded
1990/09/01 - 1993/09/01 Membrane fusion : Mechanisms of viral infection and intracelular membrane fusion
PMCT/C/BIO/214/90
Principal investigator
Center for Cell Biology , University of Coimbra , Portugal
Concluded
1986/09 - 1987/09 Calorimetric and Thermodynamic Study of the Interaction of Calcium Channel Blockers and of Dopamine Agonists and Antagonists with Liposomes and Biological Membranes
no reference
Principal investigator
Concluded

Other

Designation Funders
1986/09 - 1987/09 Determination of Thermodynamic Parameters in Biomembranes
no reference code
Principal investigator
Fundação Calouste Gulbenkian
Concluded
Outputs

Publications

Book
  1. Maria C. Pedroso de Lima. A Toxicological/Pharmacological Approach to Chemico-Biological Interactions at a Membrane Level. India: Research Signpost. 2012.
    Published • Editor
  2. Maria C. Pedroso De Lima; Nejat Düzgünes; Dick Hoekstra. Trafficking of intracellular membranes: From Molecular Sorting to Membrane Fusion. Springer-Verlag. 1995.
    Published • 10.1007/978-3-642-79547-3 • Editor
Book chapter
  1. Faneca, H.; Düzgünes, N.; Pedroso de Lima, M.C.. "Suicide gene therapy for oral squamous cell carcinoma". In Methods in Molecular Biology: Suicide Gene Therapy, 43-55. Albania: Springer, 2019.
    10.1007/978-1-4939-8922-5_4 • Editor
  2. Cardoso, Ana M.; Cardoso, Ana L; Pedroso de Lima, Maria C; Jurado, Amália S. "Non-viral therapeutic approaches for modul. "Non-viral therapeutic approaches for modulation of gene expression: nanotechnological strategies to overcome biological challenges". In Nanoparticles in the Life Sciences and Biomedicine, 409-432. Pan Stanford Publishing, 2018.
    Published
  3. De Lima, M.C.P.; Hoekstra, D.. "Liposomes, viruses, and membrane fusion". 2017.
  4. Cardoso, A.M.S.; Silva, S.G.; Luísa Do Vale, M.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S.. "Gene Delivery Mediated by Gemini Surfactants: Structure-Activity Relationships". In International Review of Neurobiology. 2016.
    10.1016/B978-0-323-41532-3.00007-5
  5. Cardoso, A.M.; Guedes, J.R.; Cardoso, A.L.; Morais, C.; Cunha, P.; Viegas, A.T.; Costa, R.; Jurado, A.; Pedroso de Lima, M.C.. "Recent Trends in Nanotechnology Toward CNS Diseases". In International Review of Neurobiology, 1-40. Elsevier, 2016.
    10.1016/bs.irn.2016.05.002
  6. Gliedes, J.; Viegas, A.T.; Pedroso de Lima, M.C.; Cardoso, A.L.. "Microglia: A double-edged sword for Alzheimer's disease". In International Review of Neurobiology. 2015.
  7. Cardoso, A.L.; Trabulo, S.M.; Duarte, S.P.; Costa, P.M.; Almeida, L.P.; de Lima, M.C.P.. "Gene silencing as a promising strategy to target CNS and neurological disorders". In International Review of Neurobiology. 2013.
  8. Trabulo, S.; Cardoso, A.L.; Cardoso, A.M.S.; Düzgünes, N.; Jurado, A.S.; De Lima, M.C.P.. "Cell-penetrating peptide-based systems for nucleic acid delivery: A biological and biophysical approach". In International Review of Neurobiology. 2012.
    10.1016/B978-0-12-391858-1.00014-9
  9. Maria C. Pedroso de Lima. "Interaction of nucleic acids with biological membranes: activation of inflammatory mechanisms". In A Toxicological/Pharmacological Approach to Chemico-Biological Interactions at a Membrane Level, 203-227. Kerala, India: Research Signpost, 2012.
    Published
  10. Maria C. Pedroso de Lima. "The role of membrane lipids in a toxicological context: Lessons from microorganism adaptive responses". In A Toxicological/Pharmacological Approach to Chemico-Biological Interactions at a Membrane Level, edited by Jurado, A.S., Maria C.Pedroso de Lima and Leonor Almeida, 1-26. Kerala, India: Research Signpost, 2012.
    Published
  11. Maria C. Pedroso de Lima. "Cationic liposome-based systems for nucleic acid delivery: from the formulation development to therapeutic applications". In Drug delivery systems: Advanced technologies potentially applicable in personalized treatments, edited by J. Coelho, 153. Springer, 2012.
    Published
  12. Moura, V.; da Silva, L.C.G.; Fonseca, N.A.; de Lima, M.C.P.; Simões, S.; Moreira, J.N.. "Emerging targets and novel nanotechnology-based strategies: Changing the treatment paradigm in oncology". In International Review of Neurobiology. 2011.
  13. Faneca, Henrique; Düzgünes, Nejat; Lima, Maria C. Pedroso. "Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery". In Methods in Molecular Biology, 425-437. Humana Press, 2010.
    Published • 10.1007/978-1-60761-447-0_29
  14. Cardoso, Ana; Trabulo, Sara; Moreira, João Nuno; Düzgünes, Nejat; de Lima, Maria C. Pedroso. "Targeted Lipoplexes for siRNA Delivery". In Methods in Enzymology, 267-287. Elsevier, 2009.
    10.1016/s0076-6879(09)65014-x
  15. Besse, Benjamin; Soria, Jean-Charles. "Gene silencing therapy in small cell lung cancer – which agents, targets and delivery systems?". In Small-Cell Carcinomas: Causes, Diagnosis, and Treatment, edited by Jonathon G. Maldonado and Mikayla K. Cervantes onathon, 1-51. Nova Science Publishers, 2009.
    10.1007/978-1-60761-524-8_12
  16. Düzgünes, N.; Simões, S.; Lopez-Mesas, M.; de Lima, M.C.P.. "Intracellular delivery of therapeutic oligonucleotides in pH-sensitive and cationic liposomes". In Methods in Molecular Biology, 253-275. New York, United States: Marcel Dekker, Inc., 2006.
    Published
  17. Maria C. Pedroso de Lima. "pH responsive liposomes: State of the art from biophysics to therapy". In MML series, edited by R. Arshady and K. Kono, 217-250. London, United Kingdom: Kentus Books, 2006.
    Published
  18. Düzgünes, N.; Simões, S.; Slepushkin, V.; Pretzer, E.; Flasher, D.; Salem, I.I.; Steffan, G.; Konopka, K.; Pedroso De Lima, M.C.. "Delivery of antiviral agents in liposomes". In Methods in Molecular Biology. 2005.
    10.1016/S0076-6879(05)91020-3
  19. Maria C. Pedroso de Lima. "Sterically stabilized pH-sensitive liposomes". In Methods in Enzymology, edited by J.N. Abelsun and M. I. Simon, 134-147. New York, United States: Academic press, 2004.
    Published
  20. Simões, S.; Pires, P.; Da Cruz, M.T.G.; Düzgünes, N.; De Lima, M.C.P.. "Gene delivery by cationic liposome-DNA complexes containing transferrin or serum albumin". In Methods in Molecular Biology. 2003.
    10.1016/S0076-6879(03)73024-9
  21. Nejat Düzgünes¸; Sérgio Simöes; Pedro Pires; Maria Pedroso de Lima. "Biophysical characterization of cationic liposome-DNA complexes and their interaction with cells". In Methods in Molecular Biology, 369-383. New York, United States: Academic Press, 2003.
    Published • 10.1201/9780203904671.ch35
  22. Maria C. Pedroso de Lima. "7Li NMR study of lithium ion transport in perfused human neuroblastoma cells.". In Lithium: Biochemical and Clinical Advances, 211-230. 1996.
    Published
  23. Maria C. Pedroso de Lima; João Ramalho-Santos; Nejat Düzgünes; Diana Flasher; Shlomo Nir. "Entry of Enveloped Viruses Into Host Cells: Fusion Activity of the Influenza Virus Hemagglutinin". 1995.
    10.1007/978-3-642-79547-3_8
  24. Catarina R. Oliveira; M. Teresa Almeida; Maria C. Pedroso de Lima. "Membrane Fusion in the Exocytotic Release of Neurotransmitters". 1995.
    10.1007/978-3-642-79547-3_20
  25. Shlomo Nir; Nejat Düzgünes; Dick Hoekstra; João Ramalho-Santos; Maria C. Pedroso de Lima. "Mass action model of virus fusion". 1995.
    10.1007/978-3-642-79547-3_9
  26. Maria C. Pedroso de Lima. "Liposomes, viruses and membrane fusion". In Liposomes as Tools in Basic Research and Industry. New York, United States: CRC Press, 1995.
    Published
  27. João Ramalho-Santos; Maria da Conceição Pedroso de Lima. "Fusion activity of influenza virus towards target membranes. pH requirements and effect of dehydrating agents". In Biological Membranes: Structure, Biogenesis and Dynamics of Biological Membranes, 313-320. Springer-Verlag, 1994.
    Published • 10.1007/978-3-642-78846-8_31
  28. Maria C. Pedroso de Lima. "Kinetics and extent of virus-cell aggregation and fusion". In Viral Fusion Mechanisms, edited by J. Bentz, 437--452. Boca Raton, United States: CRC Press, 1993.
    Published
  29. Düzgünes, Nejat; Pedroso de Lima, Maria C.; Larsen, Charles E.; Stamatatos, Leonidas; Flasher, Diana; Alford, Dennis R.; Friend, Daniel S.; Nir, Shlomo. "Fusion of Influenza, Sendai and Simian Immunodeficiency Viruses with Cell Membranes and Liposomes". In Cell and Model Membrane Interactions, 179-197. New York, United States: Plenum Press, 1991.
    Published • 10.1007/978-1-4615-3854-7_11
  30. Maria C. Pedroso de Lima. "Molecular mechanisms of enveloped viruses entry into host cells. Protein dynamics and membrane fusion". In Advances in Membrane Fluidity, 71-97. New York, United States: Academic Press, 1991.
    Published
  31. Maria C. Pedroso de Lima. "Low temperature thermal energy storage using solid-state phase change materials". In Energy Economics and Management in Industry, 151-155. Oxford, United Kingdom: Pergamon Press, 1984.
    Published
Conference paper
  1. Maria C. Pedroso de Lima. "Insights into the mechanisms involved in the cellular uptake of the S413-PV karyophilic cell penetrating peptide". Paper presented in 3rd International and 28th European Peptide Symposium, 2005.
    Published • 10.1109/nebc.2010.5458127
  2. Sharkovsky, A.N.; Berezovsky, S.A.. "Thermodynamics of phase transitions in some organic polyols.". Paper presented in 4th International Conference on Chemical Engineering, 1985.
    10.1109/coc.2000.873496
  3. de Lima, M.C.P.; Maycock, C.D.. "LOW TEMPERATURE THERMAL ENERGY STORAGE USING SOLID-STATE PHASE CHANGE MATERIALS.". Paper presented in Energy Economics and Management in Industry, Oxford, 1984.
    Published
Journal article
  1. Catarina M. Morais; Ana M. Cardoso; Ana Rita D. Araújo; Ana Reis; Pedro Domingues; Maria Rosário M. Domingues; Maria C. Pedroso de Lima; Amália S. Jurado. "Stearoyl CoA Desaturase-1 Silencing in Glioblastoma Cells: Phospholipid Remodeling and Cytotoxicity Enhanced upon Autophagy Inhibition". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms232113014.
    10.3390/ijms232113014
  2. Ana Cardoso, Catarina M Morais, Frederico Pena, Tânia Marante, Pedro Cunha, Amália S Jurado, Maria C Pedroso de Lima. "Differentiation of glioblastoma stem cells promoted by miR-128 or miR-302a overexpression enhances senescence-associated cytotoxicity of axitinib". Human Molecular Genetics 30 (2021): 160-171.
    Open access • doi: 10.1093/hmg/ddab011.
  3. Cardoso, Ana M; Morais, Catarina M; Rebelo, Olinda; Tão, Hermínio; Barbosa, Marcos; Pedroso de Lima, Maria C; Jurado, Amália S. "Downregulation of long non-protein coding RNA MVIH impairs glioblastoma cell proliferation and invasion through an miR-302a-dependent mechanism". Human Molecular Genetics 30 1 (2021): 46-64. http://dx.doi.org/10.1093/hmg/ddab009.
    10.1093/hmg/ddab009
  4. Pereira, S.P.; Santos, S.M.A.; Fernandes, M.A.S.; Deus, C.M.; Martins, J.D.; Pedroso de Lima, M.C.; Vicente, J.A.F.; Videira, R.A.; Jurado, A.S.. "Improving pollutants environmental risk assessment using a multi model toxicity determination with in vitro, bacterial, animal and plant model systems: The case of the herbicide alachlor". Environmental Pollution 286 (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85105603093&partnerID=MN8TOARS.
    10.1016/j.envpol.2021.117239
  5. Cardoso, A.M.; Morais, C.M.; Pena, F.; Marante, T.; Cunha, P.P.; Jurado, A.S.; Pedroso de Lima, M.C.. "Differentiation of glioblastoma stem cells promoted by miR-128 or miR-302a overexpression enhances senescence-associated cytotoxicity of axitinib". Human Molecular Genetics 30 3-4 (2021): 160-171. http://www.scopus.com/inward/record.url?eid=2-s2.0-85106069155&partnerID=MN8TOARS.
    10.1093/hmg/ddab011
  6. Faria, Rúben; Albuquerque, Tânia; Neves, Ana R.; Bhatt, Himanshu; Biswas, Swati; Cardoso, Ana M.; Pedroso de Lima, Maria C.; Jurado, Amália S.; Costa, Diana. "Physicochemical characterization and targeting performance of triphenylphosphonium nano-polyplexes". Journal of Molecular Liquids 316 (2020): 113873. http://dx.doi.org/10.1016/j.molliq.2020.113873.
    10.1016/j.molliq.2020.113873
  7. Morais, Catarina M.; Cardoso, Ana M.; Aguiar, Luísa; Vale, Nuno; Nóbrega, Clévio; Zuzarte, Mónica; Gomes, Paula; Pedroso de Lima, Maria C.; Jurado, Amália S.. "Lauroylated Histidine-Enriched S413-PV Peptide as an Efficient Gene Silencing Mediator in Cancer Cells". Pharmaceutical Research 37 10 (2020): 188. http://dx.doi.org/10.1007/s11095-020-02904-x.
    10.1007/s11095-020-02904-x
  8. Coutinho, Maria Francisca; Santos, Juliana Inês; S. Mendonça, Liliana; Matos, Liliana; Prata, Maria João; S. Jurado, Amália; Pedroso de Lima, Maria C.; Alves, Sandra. "Lysosomal Storage Disease-Associated Neuropathy: Targeting Stable Nucleic Acid Lipid Particle (SNALP)-Formulated siRNAs to the Brain as a Therapeutic Approach". International Journal of Molecular Sciences 21 16 (2020): 5732. http://dx.doi.org/10.3390/ijms21165732.
    10.3390/ijms21165732
  9. Morais, C.M.; Cunha, P.P.; Melo, T.; Cardoso, A.M.; Domingues, P.; Domingues, M.R.; Pedroso de Lima, M.C.; Jurado, A.S.. "Glucosylceramide synthase silencing combined with the receptor tyrosine kinase inhibitor axitinib as a new multimodal strategy for glioblastoma". Human Molecular Genetics 28 21 (2019): 3664-3679. http://www.scopus.com/inward/record.url?eid=2-s2.0-85077223998&partnerID=MN8TOARS.
    10.1093/hmg/ddz152
  10. Cabral Filho, P.E.; Cabrera, M.P.; Cardoso, A.L.C.; Santana, O.A.; Geraldes, C.F.G.C.; Santos, B.S.; Pedroso de Lima, M.C.; Pereira, G.A.L.; Fontes, A.. "Multimodal highly fluorescent-magnetic nanoplatform to target transferrin receptors in cancer cells". Biochimica et Biophysica Acta - General Subjects 1862 12 (2018): 2788-2796. http://www.scopus.com/inward/record.url?eid=2-s2.0-85052949793&partnerID=MN8TOARS.
    10.1016/j.bbagen.2018.08.014
  11. Lebre, F.; Pedroso de Lima, M.C.; Lavelle, E.C.; Borges, O.. "Mechanistic study of the adjuvant effect of chitosan-aluminum nanoparticles". International Journal of Pharmaceutics 552 1-2 (2018): 7-15. http://www.scopus.com/inward/record.url?eid=2-s2.0-85053833190&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2018.09.044
  12. Morais, C.M.; Cardoso, A.M.; Cunha, P.P.; Aguiar, L.; Vale, N.; Lage, E.; Pinheiro, M.; et al. "Acylation of the S413-PV cell-penetrating peptide as a means of enhancing its capacity to mediate nucleic acid delivery: Relevance of peptide/lipid interactions". Biochimica et Biophysica Acta - Biomembranes 1860 12 (2018): 2619-2634. http://www.scopus.com/inward/record.url?eid=2-s2.0-85054439165&partnerID=MN8TOARS.
    10.1016/j.bbamem.2018.10.002
  13. Pedro P Cunha; Pedro M Costa; Catarina M Morais; Inês R Lopes; Ana M Cardoso; Ana L Cardoso; Miguel Mano; Amália S Jurado; Maria C Pedroso de Lima. "High-throughput screening uncovers miRNAs enhancing glioblastoma cell susceptibility to tyrosine kinase inhibitors". Human Molecular Genetics 26 22 (2017): 4375-4387. https://doi.org/10.1093/hmg/ddx323.
    10.1093/hmg/ddx323
  14. Marco Craveiro; Gaspard Cretenet; Cédric Mongellaz; Maria I. Matias; Olivier Caron; Maria C. Pedroso de Lima; Valérie S. Zimmermann; et al. "Resveratrol stimulates the metabolic reprogramming of human CD4+T cells to enhance effector function". Science Signaling 10 501 (2017): eaal3024-eaal3024. https://doi.org/10.1126/scisignal.aal3024.
    10.1126/scisignal.aal3024
  15. Lebre, F.; Bento, D.; Ribeiro, J.; Colaço, M.; Borchard, G.; Pedroso De Lima, M.C.P.; Borges, O.. "Association of chitosan and aluminium as a new adjuvant strategy for improved vaccination". International Journal of Pharmaceutics 527 1-2 (2017): 103-114. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019758926&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2017.05.028
  16. Conceição, M.; Mendonça, L.; Nóbrega, C.; Gomes, C.; Costa, P.; Hirai, H.; Moreira, J.N.; et al. "Intravenous administration of brain-targeted stable nucleic acid lipid particles alleviates Machado-Joseph disease neurological phenotype". Biomaterials 82 (2016): 124-137. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956644943&partnerID=MN8TOARS.
    10.1016/j.biomaterials.2015.12.021
  17. Cabral Filho, P.E.; Cardoso, A.L.C.; Pereira, M.I.A.; Ramos, A.P.M.; Hallwas, F.; Castro, M.M.C.A.; Geraldes, C.F.G.C.; et al. "CdTe quantum dots as fluorescent probes to study transferrin receptors in glioblastoma cells". Biochimica et Biophysica Acta - General Subjects 1860 1 (2016): 28-35. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946430431&partnerID=MN8TOARS.
    10.1016/j.bbagen.2015.09.021
  18. Conceição, M.; Mendonça, L.; Nóbrega, C.; Gomes, C.; Costa, P.; Hirai, H.; Moreira, J.N.; et al. "Safety profile of the intravenous administration of brain-targeted stable nucleic acid lipid particles". Data in Brief 6 (2016): 700-705. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956617357&partnerID=MN8TOARS.
    10.1016/j.dib.2016.01.017
  19. Lebre, F.; Borchard, G.; Faneca, H.; Pedroso De Lima, M.C.; Borges, O.. "Intranasal Administration of Novel Chitosan Nanoparticle/DNA Complexes Induces Antibody Response to Hepatitis B Surface Antigen in Mice". Molecular Pharmaceutics 13 2 (2016): 472-482. http://www.scopus.com/inward/record.url?eid=2-s2.0-84957308940&partnerID=MN8TOARS.
    10.1021/acs.molpharmaceut.5b00707
  20. Cardoso, A.L.; Guedes, J.R.; De Lima, M.C.P.. "Role of microRNAs in the regulation of innate immune cells under neuroinflammatory conditions". Current Opinion in Pharmacology 26 (2016): 1-9. http://www.scopus.com/inward/record.url?eid=2-s2.0-84942921906&partnerID=MN8TOARS.
    10.1016/j.coph.2015.09.001
  21. Cruz, R.Q.; Morais, C.M.; Cardoso, A.M.; Silva, S.G.; Vale, M.L.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S.. "Enhancing glioblastoma cell sensitivity to chemotherapeutics: A strategy involving survivin gene silencing mediated by gemini surfactant-based complexes". European Journal of Pharmaceutics and Biopharmaceutics 104 (2016): 7-18. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964330230&partnerID=MN8TOARS.
    10.1016/j.ejpb.2016.04.014
  22. Guedes, J.R.; Santana, I.; Cunha, C.; Duro, D.; Almeida, M.R.; Cardoso, A.M.; de Lima, M.C.P.; Cardoso, A.L.. "MicroRNA deregulation and chemotaxis and phagocytosis impairment in Alzheimer's disease". Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring 3 (2016): 7-17. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956620147&partnerID=MN8TOARS.
    10.1016/j.dadm.2015.11.004
  23. Cardoso, A.M.; Morais, C.M.; Cruz, A.R.; Silva, S.G.; Do Vale, M.L.; Marques, E.F.; De Lima, M.C.P.; Jurado, A.S.. "New serine-derived gemini surfactants as gene delivery systems". European Journal of Pharmaceutics and Biopharmaceutics 89 (2015): 347-356. http://www.scopus.com/inward/record.url?eid=2-s2.0-84920830778&partnerID=MN8TOARS.
    10.1016/j.ejpb.2014.12.013
  24. Costa, P.M.; Cardoso, A.L.; Mano, M.; de Lima, M.C.P.. "MicroRNAs in glioblastoma: Role in pathogenesis and opportunities for targeted therapies". CNS and Neurological Disorders - Drug Targets 14 2 (2015): 222-238. http://www.scopus.com/inward/record.url?eid=2-s2.0-84930907378&partnerID=MN8TOARS.
    10.2174/1871527314666150116123610
  25. Cardoso, A.M.; Morais, C.M.; Cruz, A.R.; Cardoso, A.L.; Silva, S.G.; Do Vale, M.L.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S.. "Gemini Surfactants Mediate Efficient Mitochondrial Gene Delivery and Expression". Molecular Pharmaceutics 12 3 (2015): 716-730. http://www.scopus.com/inward/record.url?eid=2-s2.0-84924023696&partnerID=MN8TOARS.
    10.1021/mp5005349
  26. Costa, P.M.; Cardoso, A.L.; Custódia, C.; Cunha, P.; Pereira De Almeida, L.; Pedroso De Lima, M.C.. "MiRNA-21 silencing mediated by tumor-targeted nanoparticles combined with sunitinib: A new multimodal gene therapy approach for glioblastoma". Journal of Controlled Release 207 (2015): 31-39. http://www.scopus.com/inward/record.url?eid=2-s2.0-84926665383&partnerID=MN8TOARS.
    10.1016/j.jconrel.2015.04.002
  27. Maria C. Pedroso de Lima. "Early miR-155 upregulation contributes to neuroinflammation in Alzheimer's disease triple transgenic mouse model.". Human Molecular Genetics (2014): http://www.ncbi.nlm.nih.gov/pubmed/24990149.
    10.1093/hmg/ddu348
  28. Henrique Faneca; Marta Passadouro; Maria Pedroso de Lima. "MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for pancreatic cancer". International Journal of Nanomedicine (2014): 3203-3203. https://doi.org/10.2147%2Fijn.s64456.
    10.2147/ijn.s64456
  29. Kjøniksen, A.-L.; Calejo, M.T.; Zhu, K.; Cardoso, A.M.S.; De Lima, M.C.P.; Jurado, A.S.; Nyström, B.; Sande, S.A.. "Sustained release of naltrexone from Poly(N-Isopropylacrylamide) microgels". Journal of Pharmaceutical Sciences 103 1 (2014): 227-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-84890551451&partnerID=MN8TOARS.
    10.1002/jps.23780
  30. Cardoso, A.M.; Morais, C.M.; Silva, S.G.; Marques, E.F.; De Lima, M.C.P.; Jurado, M.A.S.. "Bis-quaternary gemini surfactants as components of nonviral gene delivery systems: A comprehensive study from physicochemical properties to membrane interactions". International Journal of Pharmaceutics 474 1-2 (2014): 57-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906267891&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2014.08.011
  31. Magalhães, M.; Farinha, D.; de Lima, M.C.P.; Faneca, H.. "Increased gene delivery efficiency and specificity of a lipid-based nanosystem incorporating a glycolipid". International Journal of Nanomedicine 9 (2014): 4979-4989. http://www.scopus.com/inward/record.url?eid=2-s2.0-84930260157&partnerID=MN8TOARS.
    10.2147/IJN.S69822
  32. Cardoso, A.M.; Calejo, M.T.; Morais, C.M.; Cardoso, A.L.; Cruz, R.; Zhu, K.; Pedroso De Lima, M.C.; Jurado, A.S.; Nyström, B.. "Application of thermoresponsive PNIPAAM-b-PAMPTMA diblock copolymers in siRNA delivery". Molecular Pharmaceutics 11 3 (2014): 819-827. http://www.scopus.com/inward/record.url?eid=2-s2.0-84901742907&partnerID=MN8TOARS.
    10.1021/mp400510a
  33. Passadouro, M.; Pedroso de Lima, M.C.; Faneca, H.. "MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for pancreatic cancer". International Journal of Nanomedicine 9 1 (2014): 3203-3217. http://www.scopus.com/inward/record.url?eid=2-s2.0-84903878322&partnerID=MN8TOARS.
    10.2147/IJN.S64456
  34. Farinha, D.; Pedroso De Lima, M.C.; Faneca, H.. "Specific and efficient gene delivery mediated by an asialofetuin-associated nanosystem". International Journal of Pharmaceutics 473 1-2 (2014): 366-374. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904968165&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2014.07.019
  35. Calejo, M.T.; Cardoso, A.M.S.; Kjøniksen, A.-L.; Zhu, K.; Morais, C.M.; Sande, S.A.; Cardoso, A.L.; et al. "Temperature-responsive cationic block copolymers as nanocarriers for gene delivery". International Journal of Pharmaceutics 448 1 (2013): 105-114. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875930565&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2013.03.028
  36. Calejo, M.T.; Cardoso, A.M.S.; Marques, E.F.; Araújo, M.J.; Kjøniksen, A.-L.; Sande, S.A.; de Lima, M.C.P.; Jurado, A.S.; Nyström, B.. "In vitro cytotoxicity of a thermoresponsive gel system combining ethyl(hydroxyethyl) cellulose and lysine-based surfactants". Colloids and Surfaces B: Biointerfaces 102 (2013): 682-686. http://www.scopus.com/inward/record.url?eid=2-s2.0-84867835593&partnerID=MN8TOARS.
    10.1016/j.colsurfb.2012.09.033
  37. Duarte, S.; Momier, D.; Baqué, P.; Casanova, V.; Loubat, A.; Samson, M.; Guigonis, J.-M.; et al. "Preventive cancer stem cell-based vaccination reduces liver metastasis development in a rat colon carcinoma syngeneic model". Stem Cells 31 3 (2013): 423-432. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874818661&partnerID=MN8TOARS.
    10.1002/stem.1292
  38. Gomes, C.P.; Gomes-da-Silva, L.C.; Ramalho, J.S.; de Lima, M.C.P.; Simões, S.; Moreira, J.N.. "Impact of PLK-1 silencing on endothelial cells and cancer cells of diverse histological origin". Current Gene Therapy 13 3 (2013): 189-201. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878633310&partnerID=MN8TOARS.
    10.2174/1566523211313030004
  39. Costa, P.M.; Cardoso, A.L.; Mendonça, L.S.; Serani, A.; Custódia, C.; Conceição, M.; Simões, S.; et al. "Tumor-targeted chlorotoxin-coupled nanoparticles for nucleic acid delivery to glioblastoma cells: A promising system for glioblastoma treatment". Molecular Therapy - Nucleic Acids 2 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84879940866&partnerID=MN8TOARS.
    10.1038/mtna.2013.30
  40. Costa, P.M.; De Lima, M.C.P.. "Micrornas as molecular targets for cancer therapy: On the modulation of microrna expression". Pharmaceuticals 6 10 (2013): 1195-1220. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885029680&partnerID=MN8TOARS.
    10.3390/ph6101195
  41. Gomes-Da-Silva, L.C.; Ramalho, J.S.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N.. "Impact of anti-PLK1 siRNA-containing F3-targeted liposomes on the viability of both cancer and endothelial cells". European Journal of Pharmaceutics and Biopharmaceutics 85 3 PART A (2013): 356-364. http://www.scopus.com/inward/record.url?eid=2-s2.0-84888308868&partnerID=MN8TOARS.
    10.1016/j.ejpb.2013.04.007
  42. Guedes, J.; Cardoso, A.L.C.; Pedroso De Lima, M.C.. "Involvement of MicroRNA in microglia-mediated immune response". Clinical and Developmental Immunology 2013 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84878648745&partnerID=MN8TOARS.
    10.1155/2013/186872
  43. Costa, P.M.; Cardoso, A.L.; Nóbrega, C.; Pereira de almeida, L.F.; Bruce, J.N.; Canoll, P.; Pedroso de Lima, M.C.. "MicroRNA-21 silencing enhances the cytotoxic effect of the antiangiogenic drug sunitinib in glioblastoma". Human Molecular Genetics 22 5 (2013): 904-918. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873438180&partnerID=MN8TOARS.
    10.1093/hmg/dds496
  44. Silva, S.G.; Alves, C.; Cardoso, A.M.S.; Jurado, A.S.; Pedroso De Lima, M.C.; Vale, M.L.C.; Marques, E.F.. "Synthesis of gemini surfactants and evaluation of their interfacial and cytotoxic properties: Exploring the multifunctionality of serine as headgroup". European Journal of Organic Chemistry 9 (2013): 1758-1769. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875008251&partnerID=MN8TOARS.
    10.1002/ejoc.201201396
  45. Trabulo, S.; Cardoso, A.L.; Cardoso, A.M.S.; Morais, C.M.; Jurado, A.S.; Pedroso de Lima, M.C.. "Cell-penetrating peptides as nucleic acid delivery systems: From biophysics to biological applications". Current Pharmaceutical Design 19 16 (2013): 2895-2923. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878655380&partnerID=MN8TOARS.
    10.2174/1381612811319160006
  46. Cardoso, A.M.; Trabulo, S.; Cardoso, A.L.; Maia, S.; Gomes, P.; Jurado, A.S.; Pedroso De Lima, M.C.. "Comparison of the efficiency of complexes based on S413-PV cell-penetrating peptides in plasmid DNA and siRNA delivery". Molecular Pharmaceutics 10 7 (2013): 2653-2666. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879735661&partnerID=MN8TOARS.
    10.1021/mp400078h
  47. Gomes-Da-Silva, L.C.; Fernández, Y.; Abasolo, I.; Schwartz, S.; Ramalho, J.S.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N.. "Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform". Nanomedicine 8 9 (2013): 1397-1413. http://www.scopus.com/inward/record.url?eid=2-s2.0-84893195240&partnerID=MN8TOARS.
    10.2217/nnm.12.174
  48. Duarte, S.; Loubat, A.; Momier, D.; Topi, M.; Faneca, H.; Pedroso De Lima, M.C.; Carle, G.F.; Pierrefite-Carle, V.. "Isolation of head and neck squamous carcinoma cancer stem-like cells in a syngeneic mouse model and analysis of hypoxia effect". Oncology Reports 28 3 (2012): 1057-1062. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865312688&partnerID=MN8TOARS.
    10.3892/or.2012.1904
  49. Moura, V.; Lacerda, M.; Figueiredo, P.; Corvo, M.L.; Cruz, M.E.M.; Soares, R.; De Lima, M.C.P.; Simões, S.; Moreira, J.N.. "Targeted and intracellular triggered delivery of therapeutics to cancer cells and the tumor microenvironment: Impact on the treatment of breast cancer". Breast Cancer Research and Treatment 133 1 (2012): 61-73. http://www.scopus.com/inward/record.url?eid=2-s2.0-84860334520&partnerID=MN8TOARS.
    10.1007/s10549-011-1688-7
  50. Gomes-Da-Silva, L.C.; Fonseca, N.A.; Moura, V.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N.. "Lipid-based nanoparticles for siRNA delivery in cancer therapy: Paradigms and challenges". Accounts of Chemical Research 45 7 (2012): 1163-1171. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863922166&partnerID=MN8TOARS.
    10.1021/ar300048p
  51. Cardoso, A.L.; Guedes, J.R.; Pereira de Almeida, L.; Pedroso de Lima, M.C.. "miR-155 modulates microglia-mediated immune response by down-regulating SOCS-1 and promoting cytokine and nitric oxide production". Immunology 135 1 (2012): 73-88. http://www.scopus.com/inward/record.url?eid=2-s2.0-83055176904&partnerID=MN8TOARS.
    10.1111/j.1365-2567.2011.03514.x
  52. Duarte, S.; Carle, G.; Faneca, H.; Lima, M.C.P.D.; Pierrefite-Carle, V.. "Suicide gene therapy in cancer: Where do we stand now?". Cancer Letters 324 2 (2012): 160-170. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864392111&partnerID=MN8TOARS.
    10.1016/j.canlet.2012.05.023
  53. Costa, P.M.; Cardoso, A.L.; Pereira de almeida, L.F.; Bruce, J.N.; Canoll, P.; Pedroso de lima, M.C.. "PDGF-B-mediated downregulation of miR-21: New insights into PDGF signaling in glioblastoma". Human Molecular Genetics 21 23 (2012): 5118-5130. http://www.scopus.com/inward/record.url?eid=2-s2.0-84869025536&partnerID=MN8TOARS.
    10.1093/hmg/dds358
  54. Cardoso, A.M.S.; Trabulo, S.; Cardoso, A.L.; Lorents, A.; Morais, C.M.; Gomes, P.; Nunes, C.; et al. "S4(13)-PV cell-penetrating peptide induces physical and morphological changes in membrane-mimetic lipid systems and cell membranes: Implications for cell internalization". Biochimica et Biophysica Acta - Biomembranes 1818 3 (2012): 877-888. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856059612&partnerID=MN8TOARS.
    10.1016/j.bbamem.2011.12.022
  55. Mendonça, L.S.; Pedroso De Lima, M.C.; Simöes, S.. "Targeted lipid-based systems for siRNA delivery". Journal of Drug Delivery Science and Technology 22 1 (2012): 65-73. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857427765&partnerID=MN8TOARS.
  56. Calejo, M.T.; Kjoniksen, A.-L.; Pinazo, A.; Pérez, L.; Cardoso, A.M.S.; Pedroso De Lima, M.C.; Jurado, A.S.; Sande, S.A.; Nyström, B.. "Thermoresponsive hydrogels with low toxicity from mixtures of ethyl(hydroxyethyl) cellulose and arginine-based surfactants". International Journal of Pharmaceutics 436 1-2 (2012): 454-462. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865288628&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2012.07.018
  57. Gomes-Da-Silva, L.C.; Santos, A.O.; Bimbo, L.M.; Moura, V.; Ramalho, J.S.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N.. "Toward a siRNA-containing nanoparticle targeted to breast cancer cells and the tumor microenvironment". International Journal of Pharmaceutics 434 1-2 (2012): 9-19. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864129727&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2012.05.018
  58. Duarte, S.; Faneca, H.; Lima, M.C.P.D.. "Folate-associated lipoplexes mediate efficient gene delivery and potent antitumoral activity in vitro and in vivo". International Journal of Pharmaceutics 423 2 (2012): 365-377. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856552664&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2011.12.035
  59. Lebre, F.; Borchard, G.; De Lima, M.C.P.; Borges, O.. "Progress towards a needle-free hepatitis B vaccine". Pharmaceutical Research 28 5 (2011): 986-1012. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955607675&partnerID=MN8TOARS.
    10.1007/s11095-010-0314-4
  60. Moura, M.J.; Faneca, H.; Lima, M.P.; Gil, M.H.; Figueiredo, M.M.. "In situ forming chitosan hydrogels prepared via ionic/covalent Co-cross-linking". Biomacromolecules 12 9 (2011): 3275-3284. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052771947&partnerID=MN8TOARS.
    10.1021/bm200731x
  61. Lundberg, D.; Faneca, H.; MorÁn, M.D.C.; Pedroso De Lima, M.C.; Miguel, M.D.G.; Lindman, B.. "Inclusion of a single-tail amino acid-based amphiphile in a lipoplex formulation: Effects on transfection efficiency and physicochemical properties". Molecular Membrane Biology 28 1 (2011): 42-53. http://www.scopus.com/inward/record.url?eid=2-s2.0-78651279670&partnerID=MN8TOARS.
    10.3109/09687688.2010.508879
  62. Duarte, S.; Faneca, H.; Pedroso De Lima, M.C.. "Non-covalent association of folate to lipoplexes: A promising strategy to improve gene delivery in the presence of serum". Journal of Controlled Release 149 3 (2011): 264-272. http://www.scopus.com/inward/record.url?eid=2-s2.0-79951941675&partnerID=MN8TOARS.
    10.1016/j.jconrel.2010.10.032
  63. Trabulo, S.; Cardoso, A.M.; Santos-Ferreira, T.; Cardoso, A.L.; Simões, S.; Pedroso De Lima, M.C.. "Survivin silencing as a promising strategy to enhance the sensitivity of cancer cells to chemotherapeutic agents". Molecular Pharmaceutics 8 4 (2011): 1120-1131. http://www.scopus.com/inward/record.url?eid=2-s2.0-79961082146&partnerID=MN8TOARS.
    10.1021/mp100426e
  64. Cardoso, A.M.S.; Faneca, H.; Almeida, J.A.S.; Pais, A.A.C.C.; Marques, E.F.; De Lima, M.C.P.; Jurado, A.S.. "Gemini surfactant dimethylene-1,2-bis(tetradecyldimethylammonium bromide)-based gene vectors: A biophysical approach to transfection efficiency". Biochimica et Biophysica Acta - Biomembranes 1808 1 (2011): 341-351. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649777308&partnerID=MN8TOARS.
    10.1016/j.bbamem.2010.09.026
  65. Silva-Lucca, R.A.; Faneca, H.M.S.; de Lima, M.C.P.; De Caroli, F.P.; Assis, M.L.; Sampaio, M.U.; Oliva, M.L.V.. "Interaction of proteinase inhibitors with phospholipid vesicles is modulated by pH". International Journal of Biological Macromolecules 47 4 (2010): 551-557. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956717001&partnerID=MN8TOARS.
    10.1016/j.ijbiomac.2010.07.011
  66. Mendonça, L.S.; Moreira, J.N.; De Lima, M.C.P.; Simões, S.. "Co-encapsulation of anti-BCR-ABL siRNA and imatinib mesylate in transferrin receptor-targeted sterically stabilized liposomes for chronic myeloid leukemia treatment". Biotechnology and Bioengineering 107 5 (2010): 884-893. http://www.scopus.com/inward/record.url?eid=2-s2.0-77958590068&partnerID=MN8TOARS.
    10.1002/bit.22858
  67. Mendonça, L.S.; Firmino, F.; Moreira, J.N.; De Lima, M.C.P.; Simões, S.. "Transferrin receptor-targeted liposomes encapsulating anti-BCR-ABL siRNA or asodn for chronic myeloid leukemia treatment". Bioconjugate Chemistry 21 1 (2010): 157-168. http://www.scopus.com/inward/record.url?eid=2-s2.0-74949127407&partnerID=MN8TOARS.
    10.1021/bc9004365
  68. Santos, A.O.; da Silva, L.C.G.; Bimbo, L.M.; de Lima, M.C.P.; Simões, S.; Moreira, J.N.. "Design of peptide-targeted liposomes containing nucleic acids". Biochimica et Biophysica Acta - Biomembranes 1798 3 (2010): 433-441. http://www.scopus.com/inward/record.url?eid=2-s2.0-76749124054&partnerID=MN8TOARS.
    10.1016/j.bbamem.2009.12.001
  69. Penacho, N.; Rosa, M.; Lindman, B.; Miguel, M.G.; Simões, S.; de Lima, M.C.P.. "Physicochemical properties of transferrin-associated lipopolyplexes and their role in biological activity". Colloids and Surfaces B: Biointerfaces 76 1 (2010): 207-214. http://www.scopus.com/inward/record.url?eid=2-s2.0-74149085531&partnerID=MN8TOARS.
    10.1016/j.colsurfb.2009.10.034
  70. Trabulo, S.; Cardoso, A.L.; Mano, M.; de Lima, M.C.P.. "Cell-penetrating peptides-mechanisms of cellular uptake and generation of delivery systems". Pharmaceuticals 3 4 (2010): 961-993. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952146840&partnerID=MN8TOARS.
    10.3390/ph3040961
  71. Alves, S.; Nascimento-Ferreira, I.; Dufour, N.; Hassig, R.; Auregan, G.; Nóbrega, C.; Brouillet, E.; et al. "Silencing ataxin-3 mitigates degeneration in a rat model of Machado-Joseph disease: No role for wild-type ataxin-3?". Human Molecular Genetics 19 12 (2010): 2380-2394. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955290123&partnerID=MN8TOARS.
    10.1093/hmg/ddq111
  72. Cardoso, A.L.C.; Costa, P.; de Almeida, L.P.; Simões, S.; Plesnila, N.; Culmsee, C.; Wagner, E.; Pedroso de Lima, M.C.. "Tf-lipoplex-mediated c-Jun silencing improves neuronal survival following excitotoxic damage in vivo". Journal of Controlled Release 142 3 (2010): 392-403. http://www.scopus.com/inward/record.url?eid=2-s2.0-77349098528&partnerID=MN8TOARS.
    10.1016/j.jconrel.2009.11.004
  73. Trabulo, S.; Resina, S.; Simões, S.; Lebleu, B.; Pedroso de Lima, M.C.. "A non-covalent strategy combining cationic lipids and CPPs to enhance the delivery of splice correcting oligonucleotides". Journal of Controlled Release 145 2 (2010): 149-158. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953961849&partnerID=MN8TOARS.
    10.1016/j.jconrel.2010.03.021
  74. Santos, A.O.; Pereira, J.P.; Pedroso de Lima, M.C.; Simões, S.; Moreira, J.N.. "In vitro modulation of Bcl-2 levels in small cell lung cancer cells: Effects on cell viability". Brazilian Journal of Medical and Biological Research 43 10 (2010): 1001-1009. http://www.scopus.com/inward/record.url?eid=2-s2.0-78149263712&partnerID=MN8TOARS.
    10.1590/S0100-879X2010007500099
  75. Düzgünes, N.; Faneca, H.; Lima, M.C.. "Methods to monitor liposome fusion, permeability, and interaction with cells.". Methods in molecular biology (Clifton, N.J.) 606 (2010): 209-232. http://www.scopus.com/inward/record.url?eid=2-s2.0-77649218400&partnerID=MN8TOARS.
    10.1007/978-1-60761-447-0_16
  76. Padari, K.; Koppel, K.; Lorents, A.; Hällbrink, M.; Mano, M.; Pedroso De Lima, M.C.; Pooga, M.. "S413-PV cell-penetrating peptide forms nanoparticle-like structures to gain entry into cells". Bioconjugate Chemistry 21 4 (2010): 774-783. http://www.scopus.com/inward/record.url?eid=2-s2.0-77951285705&partnerID=MN8TOARS.
    10.1021/bc900577e
  77. Penacho, N.; Simões, S.; De Lima, M.P.. "Polyethylenimine of various molecular weights as adjuvant for transfection mediated by cationic liposomes". Molecular Membrane Biology 26 4 (2009): 249-263. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650308758&partnerID=MN8TOARS.
    10.1080/09687680902766716
  78. Neves, S.; Faneca, H.; Bertin, S.; Konopka, K.; Düzgünes, N.; Pierrefite-Carle, V.; Simões, S.; Pedroso De Lima, M.C.. "Transferrin lipoplex-mediated suicide gene therapy of oral squamous cell carcinoma in an immunocompetent murine model and mechanisms involved in the antitumoral response". Cancer Gene Therapy 16 1 (2009): 91-101. http://www.scopus.com/inward/record.url?eid=2-s2.0-57649131464&partnerID=MN8TOARS.
    10.1038/cgt.2008.60
  79. Faneca, H.; Figueiredo, V.A.; Tomaz, I.; Gonçalves, G.; Avecilla, F.; Pedroso de Lima, M.C.; Geraldes, C.F.G.C.; Pessoa, J.C.; Castro, M.M.C.A.. "Vanadium compounds as therapeutic agents: Some chemical and biochemical studies". Journal of Inorganic Biochemistry 103 4 (2009): 601-608. http://www.scopus.com/inward/record.url?eid=2-s2.0-62349102649&partnerID=MN8TOARS.
    10.1016/j.jinorgbio.2008.11.004
  80. Nunes-Correia, I.; Rodríguez, J.M.; Eulálio, A.; Carvalho, A.L.; Citovsky, V.; Simões, S.; Faro, C.; Salas, M.L.; Pedroso de Lima, M.C.. "African swine fever virus p10 protein exhibits nuclear import capacity and accumulates in the nucleus during viral infection". Veterinary Microbiology 130 1-2 (2008): 47-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-46549086170&partnerID=MN8TOARS.
    10.1016/j.vetmic.2007.12.010
  81. Alves, S.; Nascimento-Ferreira, I.; Auregan, G.; Hassig, R.; Dufour, N.; Brouillet, E.; Pedroso de Lima, M.C.; et al. "Allele-specific RNA silencing of mutant ataxin-3 mediates neuroprotection in a rat model of Machado-Joseph disease". PLoS ONE 3 10 (2008): http://www.scopus.com/inward/record.url?eid=2-s2.0-54449095083&partnerID=MN8TOARS.
    10.1371/journal.pone.0003341
  82. Trabulo, S.; Mano, M.; Faneca, H.; Cardoso, A.L.; Duarte, S.; Henriques, A.; Paiva, A.; et al. "S413-PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA". Journal of Gene Medicine 10 11 (2008): 1210-1222. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149190435&partnerID=MN8TOARS.
    10.1002/jgm.1247
  83. Santos, A.; Sarmento-Ribeiro, A.B.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N.. "Simultaneous evaluation of viability and Bcl-2 in small-cell lung cancer". Cytometry Part A 73 12 (2008): 1165-1172. http://www.scopus.com/inward/record.url?eid=2-s2.0-56749165193&partnerID=MN8TOARS.
    10.1002/cyto.a.20634
  84. Faneca, H.; Faustino, A.; Pedroso de Lima, M.C.. "Synergistic antitumoral effect of vinblastine and HSV-Tk/GCV gene therapy mediated by albumin-associated cationic liposomes". Journal of Controlled Release 126 2 (2008): 175-184. http://www.scopus.com/inward/record.url?eid=2-s2.0-38749101958&partnerID=MN8TOARS.
    10.1016/j.jconrel.2007.12.005
  85. Rosa, M.; Penacho, N.; Simões, S.; Lima, M.C.P.; Lindman, B.; Miguel, M.G.. "DNA pre-condensation with an amino acid-based cationic amphiphile. A viable approach for liposome-based gene delivery". Molecular Membrane Biology 25 1 (2008): 23-34. http://www.scopus.com/inward/record.url?eid=2-s2.0-37549013769&partnerID=MN8TOARS.
    10.1080/09687680701499451
  86. Cardoso, A.L.C.; Simões, S.; de Almeida, L.P.; Plesnila, N.; Pedroso de Lima, M.C.; Wagner, E.; Culmsee, C.. "Tf-lipoplexes for neuronal siRNA delivery: A promising system to mediate gene silencing in the CNS". Journal of Controlled Release 132 2 (2008): 113-123. http://www.scopus.com/inward/record.url?eid=2-s2.0-56349102126&partnerID=MN8TOARS.
    10.1016/j.jconrel.2008.08.014
  87. Moreira, J.N.; Santos, A.; Moura, V.; De Uma, M.C.P.; Simões, S.. "Non-viral lipid-based nanoparticles for targeted cancer systemic gene silencing". Journal of Nanoscience and Nanotechnology 8 5 (2008): 2187-2204. http://www.scopus.com/inward/record.url?eid=2-s2.0-47149083118&partnerID=MN8TOARS.
    10.1166/jnn.2008.319
  88. Alves, S.; Régulier, E.; Nascimento-Ferreira, I.; Hassig, R.; Dufour, N.; Koeppen, A.; Carvalho, A.L.; et al. "Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease". Human Molecular Genetics 17 14 (2008): 2071-2083. http://www.scopus.com/inward/record.url?eid=2-s2.0-46249122383&partnerID=MN8TOARS.
    10.1093/hmg/ddn106
  89. Penacho, N.; Filipe, A.; Simões, S.; Pedroso De Lima, M.C.. "Transferrin-associated lipoplexes as gene delivery systems: Relevance of mode of preparation and biophysical properties". Journal of Membrane Biology 221 3 (2008): 141-152. http://www.scopus.com/inward/record.url?eid=2-s2.0-40549096386&partnerID=MN8TOARS.
    10.1007/s00232-008-9092-x
  90. Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simões, S.; Pedroso de Lima, M.C.. "Interaction of S413-PV cell penetrating peptide with model membranes: Relevance to peptide translocation across biological membranes". Journal of Peptide Science 13 5 (2007): 301-313. http://www.scopus.com/inward/record.url?eid=2-s2.0-34248347468&partnerID=MN8TOARS.
    10.1002/psc.842
  91. Bertin, S.; Neves, S.; Pedroso De Lima, M.; Pierrefite-Carle, V.. "Naked DNA and lipoplexes applications in cancer gene therapy,Applications de l'ADN nu ou associé àdes liposomes en thérapie génique anticancéreuse". Bulletin du Cancer 94 3 (2007): 243-252. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947501342&partnerID=MN8TOARS.
    10.1684/bdc.2007.0218
  92. Cardoso, A.L.C.; Simões, S.; de Almeida, L.P.; Pelisek, J.; Culmsee, C.; Wagner, E.; Pedroso de Lima, M.C.. "siRNA delivery by a transferrin-associated lipid-based vector: A non-viral strategy to mediate gene silencing". Journal of Gene Medicine 9 3 (2007): 170-183. http://www.scopus.com/inward/record.url?eid=2-s2.0-34047102911&partnerID=MN8TOARS.
    10.1002/jgm.1006
  93. Eulálio, A.; Nunes-Correia, I.; Salas, J.; Salas, M.L.; Simões, S.; Pedroso de Lima, M.C.. "African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times". Virus Research 130 1-2 (2007): 18-27. http://www.scopus.com/inward/record.url?eid=2-s2.0-35748981502&partnerID=MN8TOARS.
    10.1016/j.virusres.2007.05.009
  94. Bertin, S.; Neves, S.; Gavelli, A.; Baqué, P.; Brossette, N.; Simões, S.; Pedroso De Lima, M.C.; Pierrefite-Carle, V.. "Cellular and molecular events associated with the antitumor response induced by the cytosine deaminase/5-fluorocytosine suicide gene therapy system in a rat liver metastasis model". Cancer Gene Therapy 14 10 (2007): 858-866. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548606960&partnerID=MN8TOARS.
    10.1038/sj.cgt.7701075
  95. Faneca, H.; Cabrita, A.S.; Simões, S.; Pedroso de Lima, M.C.. "Evaluation of the antitumoral effect mediated by IL-12 and HSV-tk genes when delivered by a novel lipid-based system". Biochimica et Biophysica Acta - Biomembranes 1768 5 (2007): 1093-1102. http://www.scopus.com/inward/record.url?eid=2-s2.0-34047252681&partnerID=MN8TOARS.
    10.1016/j.bbamem.2006.12.017
  96. Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simões, S.; Pedroso de Lima, M.C.. "Cellular uptake of S413-PV peptide occurs upon conformational changes induced by peptide-membrane interactions". Biochimica et Biophysica Acta - Biomembranes 1758 3 (2006): 336-346. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646534241&partnerID=MN8TOARS.
    10.1016/j.bbamem.2006.01.014
  97. Eulálio, A.; Nunes-Correia, I.; Carvalho, A.L.; Faro, C.; Citovsky, V.; Salas, J.; Salas, M.L.; Simões, S.; Pedroso De Lima, M.C.. "Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals". Journal of Virology 80 3 (2006): 1393-1404. http://www.scopus.com/inward/record.url?eid=2-s2.0-31144441675&partnerID=MN8TOARS.
    10.1128/JVI.80.3.1393-1404.2006
  98. Neves, S.S.; Sarmento-Ribeiro, A.B.; Simões, S.P.; Pedroso de Lima, M.C.. "Transfection of oral cancer cells mediated by transferrin-associated lipoplexes: Mechanisms of cell death induced by herpes simplex virus thymidine kinase/ganciclovir therapy". Biochimica et Biophysica Acta - Biomembranes 1758 11 (2006): 1703-1712. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750502013&partnerID=MN8TOARS.
    10.1016/j.bbamem.2006.08.021
  99. Simões, S.; Filipe, A.; Faneca, H.; Mano, M.; Penacho, N.; Düzgünes, N.; de Lima, M.P.. "Cationic liposomes for gene delivery". Expert Opinion on Drug Delivery 2 2 (2005): 237-254. http://www.scopus.com/inward/record.url?eid=2-s2.0-23744433700&partnerID=MN8TOARS.
    10.1517/17425247.2.2.237
  100. Pedroso de Lima, M.C.; Girão da Cruz, M.T.; Cardoso, A.L.C.; Simões, S.; Pereira de Almeida, L.. "Liposomal and viral vectors for gene therapy of the central nervous system". Current Drug Targets: CNS and Neurological Disorders 4 4 (2005): 453-465. http://www.scopus.com/inward/record.url?eid=2-s2.0-23144438160&partnerID=MN8TOARS.
    10.2174/1568007054546144
  101. Mano, M.; Teodósio, C.; Paiva, A.; Simões, S.; Pedroso De Lima, M.C.. "On the mechanisms of the internalization of S413-PV cell-penetrating peptide". Biochemical Journal 390 2 (2005): 603-612. http://www.scopus.com/inward/record.url?eid=2-s2.0-25144520907&partnerID=MN8TOARS.
    10.1042/BJ20050577
  102. Fonseca, C.; Moreira, J.N.; Ciudad, C.J.; Pedroso De Lima, M.C.; Simões, S.. "Targeting of sterically stabilised pH-sensitive liposomes to human T-leukaemia cells". European Journal of Pharmaceutics and Biopharmaceutics 59 2 (2005): 359-366. http://www.scopus.com/inward/record.url?eid=2-s2.0-12344294490&partnerID=MN8TOARS.
    10.1016/j.ejpb.2004.08.012
  103. Girão da Cruz, M.T.; Cardoso, A.L.C.; de Almeida, L.P.; Simões, S.; Pedroso de Lima, M.C.. "Tf-lipoplex-mediated NGF gene transfer to the CNS: Neuronal protection and recovery in an excitotoxic model of brain injury". Gene Therapy 12 16 (2005): 1242-1252. http://www.scopus.com/inward/record.url?eid=2-s2.0-23944465946&partnerID=MN8TOARS.
    10.1038/sj.gt.3302516
  104. Teixeira, L.J.; Seabra, M.; Reis, E.; Da Cruz, M.T.G.; De Lima, M.C.P.; Pereira, E.; Miranda, M.A.; Marques, M.P.M.. "Cytotoxic activity of metal complexes of biogenic polyamines: Polynuclear platinum(II) chelates". Journal of Medicinal Chemistry 47 11 (2004): 2917-2925. http://www.scopus.com/inward/record.url?eid=2-s2.0-2542477134&partnerID=MN8TOARS.
    10.1021/jm0311238
  105. Eulálio, A.; Nunes-Correia, I.; Carvalho, A.L.; Faro, C.; Citovsky, V.; Simões, S.; De Lima, M.C.P.. "Two African swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities". Journal of Virology 78 18 (2004): 9731-9739. http://www.scopus.com/inward/record.url?eid=2-s2.0-4444281591&partnerID=MN8TOARS.
    10.1128/JVI.78.18.9731-9739.2004
  106. Piedade, J.A.P.; Mano, M.; Pedroso De Lima, M.C.; Oretskaya, T.S.; Oliveira-Brett, A.M.. "Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces". Biosensors and Bioelectronics 20 5 (2004): 975-984. http://www.scopus.com/inward/record.url?eid=2-s2.0-7644240649&partnerID=MN8TOARS.
    10.1016/j.bios.2004.06.014
  107. Girão Da Cruz, M.T.; Simões, S.; Pedroso De Lima, M.C.. "Improving lipoplex-mediated gene transfer into C6 glioma cells and primary neurons". Experimental Neurology 187 1 (2004): 65-75. http://www.scopus.com/inward/record.url?eid=2-s2.0-1842764711&partnerID=MN8TOARS.
    10.1016/j.expneurol.2003.12.013
  108. Simões, S.; Nuno Moreira, J.; Fonseca, C.; Düzgünes, N.; Pedroso De Lima, M.C.. "On the formulation of pH-sensitive liposomes with long circulation times". Advanced Drug Delivery Reviews 56 7 (2004): 947-965. http://www.scopus.com/inward/record.url?eid=2-s2.0-1942534972&partnerID=MN8TOARS.
    10.1016/j.addr.2003.10.038
  109. Ramalho-Santos, J.; Pedroso De Lima, M.C.. "The role of target membrane sialic acid residues in the fusion activity of the influenza virus: The effect of two types of ganglioside on the kinetics of membrane merging". Cellular and Molecular Biology Letters 9 2 (2004): 337-351. http://www.scopus.com/inward/record.url?eid=2-s2.0-3142546892&partnerID=MN8TOARS.
  110. Piedade, J.A.P.; Mano, M.; Pedroso de Lima, M.C.; Oretskaya, T.S.; Oliveira-Brett, A.M.. "Voltammetric behaviour of oligonucleotide lipoplexes adsorbed onto glassy carbon electrodes". Journal of Electroanalytical Chemistry 564 1-2 (2004): 25-34. http://www.scopus.com/inward/record.url?eid=2-s2.0-1342285697&partnerID=MN8TOARS.
    10.1016/j.jelechem.2003.10.008
  111. Faneca, H.; Simões, S.; Pedroso de Lima, M.C.. "Association of albumin or protamine to lipoplexes: Enhancement of transfection and resistance to serum". Journal of Gene Medicine 6 6 (2004): 681-692. http://www.scopus.com/inward/record.url?eid=2-s2.0-13644260769&partnerID=MN8TOARS.
    10.1002/jgm.550
  112. Nunes-Correia, I.; Eulálio, A.; Nir, S.; Pedroso De Lima, M.C.. "Caveolae as an additional route for influenza virus endocytosis in MDCK cells". Cellular and Molecular Biology Letters 9 1 (2004): 47-60. http://www.scopus.com/inward/record.url?eid=2-s2.0-2342439156&partnerID=MN8TOARS.
  113. Pedroso de Lima, M.C.; Neves, S.; Filipe, A.; Düzgünes, N.; Simões, S.. "Cationic liposomes for gene delivery: From biophysics to biological applications". Current Medicinal Chemistry 10 14 (2003): 1221-1231. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037805617&partnerID=MN8TOARS.
  114. Düzgünes, N.; Tros de Ilarduya, C.; Simões, S.; Zhdanov, R.I.; Konopka, K.; Pedroso de Lima, M.C.. "Cationic liposomes for gene delivery: Novel cationic lipids and enhancement by proteins and peptides". Current Medicinal Chemistry 10 14 (2003): 1213-1220. http://www.scopus.com/inward/record.url?eid=2-s2.0-18744426403&partnerID=MN8TOARS.
  115. Nunes-Correia, I.; Nir, S.; Pedroso De Lima, M.C.. "Kinetics of influenza virus fusion with the endosomal and plasma membranes of cultured cells. Effect of temperature". Journal of Membrane Biology 195 1 (2003): 21-26. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141540481&partnerID=MN8TOARS.
    10.1007/s00232-003-2040-x
  116. Marques, M.P.M.; Girão, T.; Pedroso De Lima, M.C.; Gameiro, A.; Pereira, E.; Garcia, P.. "Cytotoxic effects of metal complexes of biogenic polyamines. I. Platinum(II) spermidine compounds: Prediction of their antitumour activity". Biochimica et Biophysica Acta - Molecular Cell Research 1589 1 (2002): 63-70. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037070098&partnerID=MN8TOARS.
    10.1016/S0167-4889(01)00186-0
  117. Faneca, H.; Simões, S.; Pedroso de Lima, M.C.. "Evaluation of lipid-based reagents to mediate intracellular gene delivery". Biochimica et Biophysica Acta - Biomembranes 1567 SUPPL. (2002): 23-33. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037164699&partnerID=MN8TOARS.
    10.1016/S0005-2736(02)00545-X
  118. Nunes-Correia, I.; Eulálio, A.; Nir, S.; Düzgünes, N.; Ramalho-Santos, J.; Pedroso De Lima, M.C.. "Fluorescent probes for monitoring virus fusion kinetics: Comparative evaluation of reliability". Biochimica et Biophysica Acta - Biomembranes 1561 1 (2002): 65-75. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037133738&partnerID=MN8TOARS.
    10.1016/S0005-2736(01)00457-6
  119. Simões, S.; Fonseca, C.; Faneca, H.; Düzgünes, N.; Pedroso de Lima, M.C.. "Protein-associated lipoplexes: Novel strategies to enhance gene delivery mediated by lipid-based particles". S.T.P. Pharma Sciences 12 6 (2002): 339-344. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036855226&partnerID=MN8TOARS.
  120. Simões, Sérgio; Slepushkin, Vladimir; Düzgünes, Nejat; Pedroso de Lima, Maria C.. "On the mechanisms of internalization and intracellular delivery mediated by pH-sensitive liposomes". Biochimica et Biophysica Acta (BBA) - Biomembranes 1515 1 (2001): 23-37. http://dx.doi.org/10.1016/s0005-2736(01)00389-3.
    10.1016/s0005-2736(01)00389-3
  121. Pedroso De Lima, M.C.; Simões, S.; Pires, P.; Faneca, H.; Düzgünes, N.. "Cationic lipid-DNA complexes in gene delivery: From biophysics to biological applications". Advanced Drug Delivery Reviews 47 2-3 (2001): 277-294. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035946624&partnerID=MN8TOARS.
    10.1016/S0169-409X(01)00110-7
  122. Düzgünes, N.; Simões, S.; Konopka, K.; Rossi, J.J.; Pedroso De Lima, M.C.. "Delivery of novel macromolecular drugs against HIV-1". Expert Opinion on Biological Therapy 1 6 (2001): 949-970. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035514271&partnerID=MN8TOARS.
    10.1517/14712598.1.6.949
  123. Düzgünes, N.; Simões, S.; Slepushkin, V.; Pretzer, E.; Rossi, J.J.; De Clercq, E.; Antao, V.P.; Collins, M.L.; Pedroso de Lima, M.C.. "Enhanced inhibition of HIV-1 replication in macrophages by antisense oligonucleotides, ribozymes and acyclic nucleoside phosphonate analogs delivered in pH-sensitive liposomes". Nucleosides, Nucleotides and Nucleic Acids 20 4-7 (2001): 515-523. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034844548&partnerID=MN8TOARS.
    10.1081/NCN-100002327
  124. Ramalho-Santos, J.; Pedroso De Lima, M.C.. "Fusion and infection of influenza and sendai viruses as modulated by dextran sulfate: A comparative study". Bioscience Reports 21 3 (2001): 293-304. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035721635&partnerID=MN8TOARS.
    10.1023/A:1013282015121
  125. Girão Da Cruz, M.Teresa; Simões, S.; Pires, P.P.C.; Nir, S.; Pedroso De Lima, M.C.. "Kinetic analysis of the initial steps involved in lipoplex-cell interactions: Effect of various factors that influence transfection activity". Biochimica et Biophysica Acta - Biomembranes 1510 1-2 (2001): 136-151. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035830655&partnerID=MN8TOARS.
    10.1016/S0005-2736(00)00342-4
  126. Simões, S.; Slepushkin, V.; Pires, P.; Gaspar, R.; Pedroso De Lima, M.C.; Düzgünes, N.. "Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum". Biochimica et Biophysica Acta - Biomembranes 1463 2 (2000): 459-469. http://www.scopus.com/inward/record.url?eid=2-s2.0-0342680042&partnerID=MN8TOARS.
    10.1016/S0005-2736(99)00238-2
  127. Egas, C.; Lavoura, N.; Resende, R.; Brito, R.M.M.; Pires, E.; Pedroso De Lima, M.C.; Faro, C.. "The saposin-like domain of the plant aspartic proteinase precursor is a potent inducer of vesicle leakage". Journal of Biological Chemistry 275 49 (2000): 38190-38196. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034623993&partnerID=MN8TOARS.
    10.1074/jbc.M006093200
  128. Simões, S.; Pires, P.; Düzgünes, N.; Pedroso De Lima, M.C.. "Cationic liposomes as gene transfer vectors: Barriers to successful application in gene therapy". Current Opinion in Molecular Therapeutics 1 2 (1999): 147-157. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037856537&partnerID=MN8TOARS.
  129. Pedroso De Lima, M.C.; Simões, S.; Pires, P.; Gaspar, R.; Slepushkin, V.; Düzgünes, N.. "Gene delivery mediated by cationic liposomes: From biophysical aspects to enhancement of transfection". Molecular Membrane Biology 16 1 (1999): 103-109. http://www.scopus.com/inward/record.url?eid=2-s2.0-0345059269&partnerID=MN8TOARS.
  130. Pires, P.; Simões, S.; Nir, S.; Gaspar, R.; Düzgünes, N.; Pedroso De Lima, M.C.. "Interaction of cationic liposomes and their DNA complexes with monocytic leukemia cells". Biochimica et Biophysica Acta - Biomembranes 1418 1 (1999): 71-84. http://www.scopus.com/inward/record.url?eid=2-s2.0-0344334068&partnerID=MN8TOARS.
    10.1016/S0005-2736(99)00023-1
  131. Nunes-Correia, I.; Ramalho-Santos, J.; Nir, S.; Pedroso De Lima, M.C.. "Interactions of influenza virus with cultured cells: Detailed kinetic modeling of binding and endocytosis". Biochemistry 38 3 (1999): 1095-1101. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033579938&partnerID=MN8TOARS.
    10.1021/bi9812524
  132. Düzgünes, N.; Pretzer, E.; Simões, S.; Slepushkin, V.; Konopka, K.; Flasher, D.; Pedroso De Lima, M.C.. "Liposome-mediated delivery of antiviral agents to human immunodeficiency virus-infected cells". Molecular Membrane Biology 16 1 (1999): 111-118. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032899408&partnerID=MN8TOARS.
  133. Simões, S.; Slepushkin, V.; Pires, P.; Gaspar, R.; Pedroso De Lima, M.C.; Düzgünes, N.. "Mechanisms of gene transfer mediated by lipoplexes associated with targeting ligands or pH-sensitive peptides". Gene Therapy 6 11 (1999): 1798-1807. http://www.scopus.com/inward/record.url?eid=2-s2.0-0342265531&partnerID=MN8TOARS.
    10.1038/sj.gt.3301015
  134. Ramalho-Santos, J.; Pedroso De Lima, M.C.. "Role of a transbilayer pH gradient in the membrane fusion activity of the influenza virus hemagglutinin: Use of the R18 assay to monitor membrane merging". Biological Procedures Online 1 3 (1999): 107-113. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750619807&partnerID=MN8TOARS.
  135. Simões, S.; Slepushkin, V.; Gaspar, R.; Pedroso De Lima, M.C.; Düzgünes, N.. "Successful transfection of lymphocytes by ternary lipoplexes". Bioscience Reports 19 6 (1999): 601-609. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033513298&partnerID=MN8TOARS.
  136. Simões, S.; Slepushkin, V.; Pretzer, E.; Dazin, P.; Gaspar, R.; Pedroso De Lima, M.C.; Düzgünes, N.. "Transfection of human macrophages by lipoplexes via the combined use of transferrin and pH-sensitive peptides". Journal of Leukocyte Biology 65 2 (1999): 270-279. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033028724&partnerID=MN8TOARS.
  137. Ramalho-Santos, J.; De Lima, M.C.P.. "The influenza virus hemagglutinin: A model protein in the study of membrane fusion". Biochimica et Biophysica Acta - Reviews on Biomembranes 1376 1 (1998): 147-154. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032578032&partnerID=MN8TOARS.
    10.1016/S0304-4157(98)00002-1
  138. Bernardes, C.; António, A.; Pedroso De Lima, M.C.; Valdeira, M.L.. "Cholesterol affects African swine fever virus infection". Biochimica et Biophysica Acta - Lipids and Lipid Metabolism 1393 1 (1998): 19-25. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032584552&partnerID=MN8TOARS.
    10.1016/S0005-2760(98)00051-4
  139. Simões, S.; Slepushkin, V.; Caspar, R.; Pedroso de Lima, M.C.; Düzgünes, N.. "Gene delivery by negatively charged ternary complexes of DNA, cationic liposomes and transferrin or fusigenic peptides". Gene Therapy 5 7 (1998): 955-964. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031825183&partnerID=MN8TOARS.
  140. Nunes-Correia, I.; Ramalho-Santos, J.; Pedroso De Lima, M.C.. "Sendai virus fusion activity as modulated by target membrane components". Bioscience Reports 18 2 (1998): 59-68. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031727161&partnerID=MN8TOARS.
    10.1023/A:1020180109275
  141. Slepushkin, V.A.; Simões, S.; Dazin, P.; Newman, M.S.; Guo, L.S.; De Lima, M.C.P.; Düzgünes, N.. "Sterically stabilized pH-sensitive liposomes. Intracellular delivery of aqueous contents and prolonged circulation in vivo". Journal of Biological Chemistry 272 4 (1997): 2382-2388. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031032815&partnerID=MN8TOARS.
    Published • 10.1074/jbc.272.4.2382
  142. Fires, P.; Simos, S.; Caspar, R.; Nir, S.; Dzgnes, N.; Lima, M.C.. "Interaction of cationic liposomes with cells: Effect of several factors on liposome-cell fusion". FASEB Journal 11 9 (1997): http://www.scopus.com/inward/record.url?eid=2-s2.0-33750172630&partnerID=MN8TOARS.
  143. Ramalho-Santos, J.; Almeida, M.T.; De Pedroso Lima, M.C.. "The influenza virus hemagglutinin: a model protein in the study of membrane fusion". FASEB Journal 11 9 (1997): http://www.scopus.com/inward/record.url?eid=2-s2.0-33750094182&partnerID=MN8TOARS.
  144. Almeida, M.T.; Ramalho-Santos, J.; Oliveira, C.R.; Pedroso De Lima, M.C.. "Evidence that synaptobrevin is involved in fusion between synaptic vesicles and synaptic plasma membrane vesicles". Biochemical and Biophysical Research Communications 236 1 (1997): 184-188. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031561140&partnerID=MN8TOARS.
    10.1006/bbrc.1997.6928
  145. Ramalho-Santos, J.; Pedroso De Lima, M.C.. "Fusion activity of the influenza virus hemagglutinin does not require a transbilayer pH gradient". Biochimica et Biophysica Acta - Biomembranes 1330 2 (1997): 194-198. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030778514&partnerID=MN8TOARS.
    10.1016/S0005-2736(97)00166-1
  146. Simoes, S.; Slepushkin, V.; Gaspar, R.; Pedroso de Lima, M.C.; Duzgunes, N.. "Enhancement of cationic liposome-mediated gene delivery by transferrin and fusogenic peptides". Proceedings of the Controlled Release Society 24 (1997): 659-660. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030800943&partnerID=MN8TOARS.
  147. Da Silva, C.P.; De Oliveira, C.R.; De Lima, M.C.P.. "Intracellular Ca2+ homeostasis in human leukemic T-lymphocytes is affected by anticancer drugs: Implications for the regulation of leukemic cell growth". Journal of Experimental and Clinical Cancer Research 15 2 (1996): 129-138. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029816963&partnerID=MN8TOARS.
  148. Da Silva, C.P.; De Oliveira, C.R.; De Lima, M.D.C.P.. "Apoptosis as a mechanism of cell death induced by different chemotherapeutic drugs in human leukemic T-lymphocytes". Biochemical Pharmacology 51 10 (1996): 1331-1340. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029947269&partnerID=MN8TOARS.
    10.1016/0006-2952(96)00041-X
  149. Ramalho-Santos, J.; De Pedroso Lima, M.C.; Nir, S.. "Partial fusion activity of influenza virus toward liposomes and erythrocyte ghosts is distinct from viral inactivation". Journal of Biological Chemistry 271 39 (1996): 23902-23906. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029842067&partnerID=MN8TOARS.
    10.1074/jbc.271.39.23902
  150. Alpoim, M.C.; Geraldes, C.F.; Oliveira, C.R.; Lima, M.C.. "Molecular mechanisms of chromium toxicity oxidation of hemoglobin". Biochemical Society Transactions 23 2 (1995): http://www.scopus.com/inward/record.url?eid=2-s2.0-0029010431&partnerID=MN8TOARS.
  151. Zachariah, C.; Defreitas, D.M.; Castro, M.M.C.A.; Geraldes, C.F.G.C.; Lima, M.C.P.; Oliveira, C.R.. "The Use of Microcarrier Beads in Ion Transport NMR Studies of Perfused Cells". Journal of Magnetic Resonance, Series B 108 1 (1995): 81-85. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029336712&partnerID=MN8TOARS.
    10.1006/jmrb.1995.1106
  152. Bernardes, C.; Valdeira, M.L.; Ramalho-Santos, J.; De Lima, M.C.P.. "Fusion activity of African swine fever virus towards target membranes: Lipid dependence and effect of dehydrating agents". Biochemistry and Molecular Biology International 37 3 (1995): 481-488. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029555561&partnerID=MN8TOARS.
  153. Pedroso de Lima, M.C.; Ramalho-Santos, J.; Flasher, D.; Slepushkin, V.A.; Nir, S.; Düzgunes, N.. "Target cell membrane sialic acid modulates both binding and fusion activity of influenza virus". BBA - Biomembranes 1236 2 (1995): 323-330. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029026187&partnerID=MN8TOARS.
    10.1016/0005-2736(95)00067-D
  154. Aureliano, M.; Pedroso, M.C.; De Lima; Carvalho, A.P.; Pires, E.M.V.. "Effect of myosin phosphorylation on actomyosin ATPase activity: a flow microcalorimetric study". Thermochimica Acta 258 C (1995): 59-66. http://www.scopus.com/inward/record.url?eid=2-s2.0-4243321608&partnerID=MN8TOARS.
    10.1016/0040-6031(94)02192-Q
  155. Ramalho-Santos, J.; Negrao, R.; Da Conceicao Pedroso de Lima, M.. "Fusion of Sendai virus with model membranes as affected by dehydrating agents and pH: Correlation between viral envelope hydrophobicity and fusion activity". Biochemistry and Molecular Biology International 32 6 (1994): 1121-1127. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028352917&partnerID=MN8TOARS.
  156. Ramalho-Santos, J.; Negrão, R.; Lima, M.d.C.P.d.. "Role of hydrophobic interactions in the fusion activity of influenza and sendai viruses towards model membranes". Bioscience Reports 14 1 (1994): 15-24. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028243606&partnerID=MN8TOARS.
    10.1007/BF01901634
  157. da Silva, C.P.; de Oliveira, C.R.; de Lima, M.d.C.P.. "In vitro assay of the effects of anticancer drugs on leukemic T-cells by flow microcalorimetry". International Journal of Pharmaceutics 111 1 (1994): 25-30. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028067072&partnerID=MN8TOARS.
    10.1016/0378-5173(94)90398-0
  158. Almeida, M.T.; Ramalho-Santos, J.; Oliveira, C.R.; Pedroso de Lima, M.C.. "Parameters affecting fusion between liposomes and synaptosomes. Role of proteins, lipid peroxidation, pH and temperature". The Journal of Membrane Biology 142 2 (1994): 217-222. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027993101&partnerID=MN8TOARS.
    10.1007/BF00234943
  159. Ramalho-Santos, J.; Nir, S.; Düzgünes, N.; De Carvalho, A.P.; De Lima, M.D.C.P.. "A common mechanism for influenza virus fusion activity and inactivation". Biochemistry 32 11 (1993): 2771-2779. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027417786&partnerID=MN8TOARS.
  160. SARMENTO, A.B.; DE LIMA, M.C.P.; OLIVEJRA, C.R.. "Partition of Dopamine Antagonists into Synthetic Lipid Bilayers: the Effect of Membrane Structure and Composition". Journal of Pharmacy and Pharmacology 45 7 (1993): 601-605. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027313440&partnerID=MN8TOARS.
    10.1111/j.2042-7158.1993.tb05660.x
  161. PEDROSO DE LIMA, M.d.C.; RAMALHO-SANTOS, J.; MARTINS, M.d.F.; PATO DE CARVALHO, A.; BAIROS, V.; NIR, S.. "Kinetic modeling of Sendai virus fusion with PC-12 cells: Effect of pH and temperature on fusion and viral inactivation". European Journal of Biochemistry 205 1 (1992): 181-186. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026611884&partnerID=MN8TOARS.
    10.1111/j.1432-1033.1992.tb16766.x
  162. Brandao, F.O.; Silva, C.P.; De Lima, M.C.P.; Oliveira, C.R.. "Prediction of the cytotoxic effects of methotrexate on human T-lymphoblastic leukemia cells". Journal of Experimental and Clinical Cancer Research 11 1 (1992): 51-62. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026778561&partnerID=MN8TOARS.
  163. Pereira da Silva, C.M.; Pedroso de Lima, M.C.. "Application of flow microcalorimetry to the study of dehydrofolate reductase activities in crude tissue homogenates". Journal of Thermal Analysis 38 4 (1992): 821-833. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249838370&partnerID=MN8TOARS.
    10.1007/BF01979414
  164. Duzgunes, N.; Pedroso de Lima, M.C.; Stamatatos, L.; Flasher, D.; Alford, D.; Friend, D.S.; Nir, S.. "Fusion activity and inactivation of influenza virus: Kinetics of low pH-induced fusion with cultured cells". Journal of General Virology 73 1 (1992): 27-37. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026504652&partnerID=MN8TOARS.
  165. Seppen, J.; Ramalho-Santos, J.; de Carvalho, A.P.; ter Beest, M.; Kok, J.W.; Pedroso de Lima, M.C.; Hoekstra, D.. "Interaction of clathrin with large unilamellar phospholipid vesicles at neutral pH. Lipid dependence and protein penetration". BBA - Biomembranes 1106 1 (1992): 209-215. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026587918&partnerID=MN8TOARS.
    10.1016/0005-2736(92)90240-M
  166. Pedroso de Lima, M.C.; Nir, S.; Flasher, D.; Klappe, K.; Hoekstra, D.; Düzgünes, N.. "Fusion of Sendai virus with human HL-60 and CEM cells: different kinetics of fusion for two isolates". BBA - Biomembranes 1070 2 (1991): 446-454. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025933996&partnerID=MN8TOARS.
    10.1016/0005-2736(91)90085-M
  167. Silva, C.M.P.; De Lima, M.C.P.; Oliveira, C.R.; Carvlho, A.P.. "A flow microcalorimetric study of the inhibition of acetylcholinesterase by catecholamine derivatives". Thermochimica Acta 179 C (1991): 221-230. http://www.scopus.com/inward/record.url?eid=2-s2.0-0347785235&partnerID=MN8TOARS.
    10.1016/0040-6031(91)80351-I
  168. Shlomo Nir; Nejat Düzgünes; Maria C. Pedroso De Lima; Dick Hoekstra. "Fusion of enveloped viruses with cells and liposomes". Cell Biophysics 17 2 (1990): 181-201. https://doi.org/10.1007%2Fbf02990496.
    10.1007/bf02990496
  169. Nir, S.; Düzgünes, N.; Pedroso De Lima, M.C.; Hoekstra, D.. "Fusion of enveloped viruses with cells and liposomes - Activity and inactivation". Cell Biophysics 17 2 (1990): 181-201. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025641298&partnerID=MN8TOARS.
    10.1007/BF02990496
  170. Pedroso de Lima, M.C.; Chiche, B.H.; Debs, R.J.; Düzgünes, N.. "Interaction of antimycobacterial and anti-pneumocystis drugs with phospholipid membranes". Chemistry and Physics of Lipids 53 4 (1990): 361-371. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025266029&partnerID=MN8TOARS.
    10.1016/0009-3084(90)90034-O
  171. Oliveira, C.R.; Lima, M.C.P.; Carvalho, C.A.M.; Leysen, J.E.; Carvalho, A.P.. "Partition coefficients of dopamine antagonists in brain membranes and liposomes". Biochemical Pharmacology 38 13 (1989): 2113-2120. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024340655&partnerID=MN8TOARS.
    10.1016/0006-2952(89)90065-8
  172. Carvalho, C.M.; Oliveira, C.R.; Lima, M.P.; Leysen, J.E.; Carvalho, A.P.. "Partition of ca2+ antagonists in brain plasma membranes". Biochemical Pharmacology 38 13 (1989): 2121-2127. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024475173&partnerID=MN8TOARS.
    10.1016/0006-2952(89)90066-X
  173. Shavnin, S.A.; Pedroso de Lima, M.C.; Fedor, J.; Wood, P.; Bentz, J.; Düzgünes, N.. "Cholesterol affects divalent cation-induced fusion and isothermal phase transitions of phospholipid membranes". BBA - Biomembranes 946 2 (1988): 405-416. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024262811&partnerID=MN8TOARS.
    10.1016/0005-2736(88)90416-6
  174. Beezer, A.E.; Gooch, C.A.; Hunter, W.H.; Lima, M.C.P.; Smith, B.V.. "Quantitative structure-activity relationships: a group additivity scheme for biological response of E. coli to the action of o-, m- and p-alkoxyphenol". International Journal of Pharmaceutics 38 1-3 (1987): 251-254. http://www.scopus.com/inward/record.url?eid=2-s2.0-0023628827&partnerID=MN8TOARS.
    10.1016/0378-5173(87)90122-0
  175. Beezer, A.E.; Lima, M.C.P.; Fox, G.G.; Hunter, W.H.; Smith, B.V.. "Solution thermodynamics for o-alkoxyphenols in water and in water-alcohol systems". Thermochimica Acta 116 C (1987): 329-335. http://www.scopus.com/inward/record.url?eid=2-s2.0-39349096788&partnerID=MN8TOARS.
    10.1016/0040-6031(87)88193-5
  176. Beezer, A.E.; Lima, M.C.P.; Fox, G.G.; Arriaga, P.; Hunter, W.H.; Smith, B.V.. "Microcalorimetric measurement of the enthalpies of transfer of a series of ortho- and para-alkoxyphenols from water to octan-1-ol and from isotonic solution to Escherichia coli cells". Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases 83 9 (1987): 2705-2707. http://www.scopus.com/inward/record.url?eid=2-s2.0-0040404893&partnerID=MN8TOARS.
    10.1039/F19878302705
  177. Schreiber, D.R.; De Lima, M.C.P.; Pitzer, K.S.. "Electrical conductivity, viscosity, and density of a two-component ionic system at its critical point". Journal of Physical Chemistry 91 15 (1987): 4087-4091. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001151523&partnerID=MN8TOARS.
  178. Beezer, A.E.; Volpe, P.L.O.; Lima, M.C.P.; Hunter, W.H.. "Solution thermodynamics for alkoxy phenols in alcohol and in water-alcohol systems". Journal of Solution Chemistry 15 4 (1986): 341-348. http://www.scopus.com/inward/record.url?eid=2-s2.0-34250123191&partnerID=MN8TOARS.
    10.1007/BF00648888
  179. Pitzer, K.S.; De Lima, M.C.P.; Schreiber, D.R.. "Critical point and phase separation for an ionic system". Journal of Physical Chemistry 89 10 (1985): 1854-1855. http://www.scopus.com/inward/record.url?eid=2-s2.0-0039672088&partnerID=MN8TOARS.
  180. de Lima, M.C.P.; Pitzer, K.S.. "Thermodynamics of saturated electrolyte mixtures of NaCl with Na2SO4 and with MgCl2". Journal of Solution Chemistry 12 3 (1983): 187-199. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847144669&partnerID=MN8TOARS.
    10.1007/BF00648056
  181. de Lima, M.C.P.; Pitzer, K.S.. "Thermodynamics of saturated aqueous solutions including mixtures of NaCl, KCl, and CsCl". Journal of Solution Chemistry 12 3 (1983): 171-185. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001071681&partnerID=MN8TOARS.
    10.1007/BF00648055
  182. Freund, P.L.; Conceiçâo, M.; Lima, P.; Spiro, M.. "An investigation of non-faradaic electrocatalysis". Journal of Electroanalytical Chemistry 133 2 (1982): 241-252. http://www.scopus.com/inward/record.url?eid=2-s2.0-49049131731&partnerID=MN8TOARS.
    10.1016/0368-1874(82)85141-1
  183. Traca, A.; Lima, M.C.P.. "A high-voltage constant-current generator". Journal of Physics E: Scientific Instruments 13 4 (1980): 387-388. http://www.scopus.com/inward/record.url?eid=2-s2.0-0019008887&partnerID=MN8TOARS.
    10.1088/0022-3735/13/4/006
  184. Lima, M.C.P.; Kumarasinghe, S.; Spiro, M.. "Transference numbers of a 2:2 electrolyte: Magnesium sulphate in water at 25°C". Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases 74 (1978): 1036-1044. http://www.scopus.com/inward/record.url?eid=2-s2.0-37049106705&partnerID=MN8TOARS.
    10.1039/F19787401036
  185. Conceição, M.; Lima, P.; Spiro, M.. "Analysis of organic amines and acids for water". Analytica Chimica Acta 81 2 (1976): 429-431. http://www.scopus.com/inward/record.url?eid=2-s2.0-49549135217&partnerID=MN8TOARS.
    10.1016/S0003-2670(01)82042-0
Newsletter article
  1. Maria C. Pedroso de Lima. "Peptide Therapeutics in Portugal", The European Peptide Society , 2018

Intellectual property

Patent
  1. J.N. Moreira, V. Moura, M.C. Pedroso de Lima and S. Simões. 2018. "pH-sensitive liposome binding to nucleolin". Portugal.
    Granted/Issued
  2. J.N. Moreira, V. Moura, M.C. Pedroso de Lima and S. Simões. 2014. "Targeted drug delivery to human diseases and disorders". United States.
    Granted/Issued
  3. J.N. Moreira, V. Moura, M.C. Pedroso de Lima and S. Simões. 2013. "Targeted delivery to human diseases and disorders". United States.
    Granted/Issued
  4. J.N. Moreira, V. Moura, M.C. Pedroso de Lima and S. Simões. 2012. "Targeted drug delivery to human diseases and disorders". United States.
    Granted/Issued
  5. N. Düzgünes, S. Simões, V. Slepushkin and M.C. Pedroso de Lima. 2001. "Non-ligand polypeptide and liposome complexes as intracellular delivery vehicles". United States.
    Granted/Issued
Activities

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2012 - Current Membrane Lipid Composition and Structure Remodeling by Cell Penetrating Peptide-based Drug Carriers. A Membrane Lipid Therapeutic Strategy for Cancer Diseases
Co-supervisor of Catarina Mendes Morais
Biociências (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2012 - 2017 Development of chitosan-based nanoparticles for nasal immunization against hepatitis B
Co-supervisor of Filipa Lebre
Ciências Farmacêuticas (PhD)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2013 - 2016 Non-viral silencing of Machado-Joseph disease through the systemic route
Co-supervisor of Mariana Oliveira Conceição
Ciências Farmacêuticas (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2012 - 2015 Inflammation in Alzheimer's disease: Deregulation and modulation of miRNA expression in the mononuclear phagocytic system
Co-supervisor of Joana Ribeiro Guedes
Biologia Experimental e Biomedicina (PhD)
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2009/04 - 2014/12 MicroRNA modulation in combination with chemotherapeutic drugs as a novel therapeutic strategy for pancreatic cancer
Co-supervisor of Marta Daniela Passadouro Caetano
Biociências-especialização em Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2010 - 2014 Nucleic acid delivery systems: from biophysics to biological activity
Co-supervisor of Ana Maria Sequeira Cardoso
Biociências-especialização em Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2010 - 2013 MicroRNAs as molecular targets for non-viral gene therapy of glioblastoma: development of a lipid-based nanosystem for nucleic acid delivery to brain tumor cells
Supervisor of Pedro Miguel Coutinho da Costa
Bioquímica-Tecnologia Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2009 - 2012 On the development of antitumoural therapeutic strategies: Suicide gene therapy mediated by lipid-based systems and cancer stem cell-based vaccination
Supervisor of Sónia Patrícia Duarte
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2008 - 2011 Development of a Novel Approach for Anti-Tumor Therapy Based on the S413-PV Cell Penetrating Peptide to Improve the Intracellular Delivery of Therapeutic Proteins and Nucleic Acids
Supervisor of Sara Maria Trabulo
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2006 - 2010 Targeting of therapeutic genes by new liposome formulations: Evaluation of their efficacy in tumor models
Co-supervisor of Liliana Simões Mendonça
Farmácia (PhD)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2004 - 2010 Suicide gene therapy for cancer: Anti-tumoral activity mediated by lipoplexes and cellular and molecular mechanisms
Supervisor of Sílvia Sousa Neves
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2002 - 2010 Development of new cationic liposome-based systems to promote gene delivery: Physicochemical characterization and evaluation of biological activity
Supervisor of Nuno Miguel Penacho Pereira
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2005 - 2008 Gene silencing as a promising strategy for neuroprotection: On the development of a lipid-based system for the delivery of siRNAs to the central nervous system
Supervisor of Ana Luísa Colaço Cardoso
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2004 - 2008 Gene Therapy for Vascular Diseases: Development of Vectors Intracelular Administration of Genes for Angiogenic Factors
Co-supervisor of Ana Filipe
Farmácia (PhD)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2003 - 2007 Interaction of Lipid-Enveloped Viruses with Cultured Cells: From Early Phases to Nuclear Translocation
Supervisor of Isabel Nunes Correia
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2003 - 2006 Development of New Liposomal Formulations to Mediate Delivery of Antisense Oligonucleotides for Treatment of Human Leukemia
Co-supervisor of Cristina Isabel Fonseca
Farmácia (PhD)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2002 - 2006 Cell penetrating Peptides as Promising Systems for Nucleic Acid Delivery and Biological Activity of the S4(13)PV Peptide
Supervisor of Miguel Luís Mano
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2002 - 2006 Nucleo-Cytoplasmic Transport of Structural proteins of African swine Fever Virus: Mechanisms and Virologic Relevance of the Protein p37
Supervisor of Ana Sofia Bregieiro Eulálio
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2001 - 2005 New Lipid-Based Systems for Aplication in Antitumor Gene Therapy
Supervisor of Henrique Manuel dos Santos faneca
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2001 - 2005 Optimization of Cationic Liposome /DNA Complexes for Transfection of Neuronal Cells
Supervisor of Maria Teresa Girão da Cruz
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2000 - 2004 Cationic Liposome/DNA Complexes for Application in Gene Therapy: Mechanisms of Internalization Intracellular Delivery of Genetic Material
Supervisor of Pedro de Pina Catarino Pires
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
1995 - 1999 The Use of Fusogenic Liposomes in Gene Therapy
Co-supervisor of Sérgio Paulo de Magalhães Simões
Farmácia (PhD)
Universidade de Coimbra Faculdade de Farmácia, Portugal
1994 - 1997 Fusion Activity of Lipid-Enveloped Viruses: Membrane Fusion promoted by Influenza and Sendai Viruses
Supervisor of João Ramalho de Sousa Santos
Biologia (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
1993 - 1997 In vitro Studies of Infection and Fusion Mechanisms of African Swine Fever Virus
Co-supervisor of Catarina Bernardes
Ciências Farmacêuticas (PhD)
Universidade de Lisboa Faculdade de Farmácia, Portugal
1992 - 1995 Biochemical Effects of Anticancer Drugs in Human Leukemia Cells: Study in Limphoblastic Cultured Cells
Supervisor of Cristina Isabel Pereira da Silva
Bioquímica (PhD)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal

Association member

Society Organization name Role
2020 - Current European Peptide Society Portuguese Network of EVs (Portuguese Network for Studies of Extracellular Vesicles (EVs).

Evaluation committee

Activity description
Role
Institution / Organization Funding entity
2021/02 - Current Evaluator of research grant proposals - National Science Centre, Poland
Evaluator
Narodowe Centrum Nauki , Poland
2016 - Current Evaluator of research grant proposals - The Teresa Rosenbaum Golden Charitable Trust
Evaluator
Rosetrees Trust, United Kingdom
2016 - Current Evaluator of research grant proposals - China Israel Cooperative Scientific Research on Brain Sciences
Evaluator
Ministry of Science Technology and Space, Israel
2012 - Current Evaluator of research proposals - Acordos Bilaterais
Evaluator
Fundação para a Ciência e a Tecnologia, Portugal
Distinctions

Award

2013 2013 | International Exiqon award
Exiqon - Grant Programme: Copenhagen, DK 2013 , Denmark