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Identification

Personal identification

Full name
Nils Meiresonne

Citation names

  • Meiresonne, Nils

Author identifiers

Ciência ID
9E13-660E-4D26
ORCID iD
0000-0001-9184-4361

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology

Languages

Language Speaking Reading Writing Listening Peer-review
Dutch (Mother tongue)
English
Education
Degree Classification
2013/03/01 - 2019/10/22
Concluded
Molecular Microbiology (Doktor (PhD))
Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
2009/09/01 - 2012/05/31
Concluded
Medical Biology (Master)
Major in Microbiology
Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
8.0, Good, A-
2006/09/01 - 2009/08/31
Concluded
Biomedical Sciences (Bachelor)
Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
7.5, Good, B+
2002/09/01 - 2006/07/20
Concluded
Biology Teacher (Bachelor)
Major in Biology Teacher
Hogeschool van Amsterdam Faculteit Onderwijs en Opvoeding, Netherlands
Affiliation

Science

Category
Host institution
Employer
2019/10/23 - Current Postdoc (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013/03/01 - 2019/10/22 Researcher (Research) Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
2012/09 - 2013/02 Researcher (Research) Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
2011/01 - 2012/01 Research Trainee (Research) Academic Medical Centre, Department of Medical Microbiology, Research Bacteriology, Netherlands
2009/09 - 2010/03 Research Trainee (Research) Academic Medical Centre, Department of Human Genetics, Netherlands
2009/02 - 2009/07 Research Trainee (Research) Academic Medical Centre, Department of Human Genetics, Netherlands
2007/07 - 2007/08 Research Trainee (Research) Academic Medical Centre, Department of Clinical Immunology and Rheumatology, Netherlands

Others

Category
Host institution
Employer
2008 - 2013/02 Laboratory Technician Onze Lieve Vrouwe Gasthuis, Medische Microbiologie, Netherlands
Projects

Grant

Designation Funders
2018/03/01 - Current Exploring the bacterial cell cycle to re-sensitize antibiotic-resistant bacteria
info:eu-repo/grantAgreement/EC/H2020/771709/EU
Post-doc Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
European Commission
Ongoing
2016 - 2018 Human Frontier Science Program (RGP0034/2013)
RGP0034/2013
PhD Student Fellow
Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
National Science Foundation
Concluded
2013/03/01 - 2017/02/28 Multifunctional antibacterial periplasmic protein interaction analysis (MAPPIA)
PhD Student Fellow
Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Netherlands
Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Contract

Designation Funders
2023/05 - Current Timing protein interactions that drive bacterial cell cycle progression.
Post-doc Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Ongoing
Outputs

Publications

Conference poster
  1. Meiresonne, Nils. Corresponding author: Meiresonne, Nils. "Visualizing protein interactions by FLIM-FRET". Paper presented in Bacterial cell biophysics: DNA replication, growth, division, size and shape, 2022.
  2. Meiresonne, Nils. "Visualising protein interactions in Staphylococcus aureus using FLIM-FRET". Paper presented in EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology, 2021.
  3. Xiaolong Liu; Meiresonne, Nils. "FtsW activity and lipid II synthesis are required for recruitment of MurJ to midcell during cell division in E. coli". Paper presented in Gordon Research Seminar and Conference “Bacterial Cell Surfaces” Mount snow, Vermont, USA, 2018.
  4. Meiresonne, Nils. "Illumination protein interactions in the periplasm". Paper presented in Gordon Research Seminar and Conference “Bacterial Cell Surfaces” Mount snow, Vermont, USA, 2018.
  5. Xiaolong Liu; Meiresonne, Nils. "The combined activities of FtsW and MurJ are required for lipid II translocation during cell division in E. coli". Paper presented in EMBO Workshop "Bacterial cell division: Orchestrating the ring cycle" Prague, Czech Republic, 2016.
  6. Meiresonne, Nils. "Periplasmic Protein Interactions". Paper presented in EMBO meeting: New Approaches and Concepts in Microbiology, EMBL, Heidelberg, Germany, 2013.
  7. Meiresonne, Nils. "Periplasmic Protein Interactions". Paper presented in Dutch Biophysics 2013, 2013.
Journal article
  1. Meiresonne, Nils Y.; den Blaauwen, Tanneke. "The In Vitro Non-Tetramerizing ZapAI83E Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli". International Journal of Molecular Sciences 21 9 (2020): 3130. http://dx.doi.org/10.3390/ijms21093130.
    Accepted • 10.3390/ijms21093130
  2. Meiresonne, Nils; Consoli, Elisa; Mertens, Laureen; den Blaauwen, Tanneke. "Detection of in vivo Protein Interactions in All Bacterial Components by Förster Resonance Energy Transfer with the Superfolder mTurquoise2 ox-mNeongreen FRET Pair". BIO-PROTOCOL 9 23 (2019): http://dx.doi.org/10.21769/bioprotoc.3448.
    10.21769/bioprotoc.3448
  3. Begoña Monterroso; Silvia Zorrilla; Marta Sobrinos-Sanguino; Miguel Ángel Robles-Ramos; Carlos Alfonso; Bill Söderström; Nils Y. Meiresonne; et al. "The Bacterial DNA Binding Protein MatP Involved in Linking the Nucleoid Terminal Domain to the Divisome at Midcell Interacts with Lipid Membranes". mBio (2019): https://doi.org/10.1128/mBio.00376-19.
    10.1128/mBio.00376-19
  4. Meiresonne, Nils Y.; Consoli, Elisa; Mertens, Laureen M.Y.; Chertkova, Anna O.; Goedhart, Joachim; den Blaauwen, Tanneke. "Superfolder mTurquoise2 ox optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets". Molecular Microbiology 111 4 (2019): 1025-1038. http://dx.doi.org/10.1111/mmi.14206.
    10.1111/mmi.14206
  5. Winfried Roseboom; Madhvi G. Nazir; Nils Meiresonne; Tamimount Mohammadi; Jolanda Verheul; Hansuk Buncherd; Alexandre M.J.J. Bonvin; et al. "Mapping the Contact Sites of the Escherichia coli Division-Initiating Proteins FtsZ and ZapA by BAMG Cross-Linking and Site-Directed Mutagenesis". International Journal of Molecular Sciences (2018): http://www.mdpi.com/1422-0067/19/10/2928.
    10.3390/ijms19102928
  6. Liu, Xiaolong; Meiresonne, Nils Y.; Bouhss, Ahmed; den Blaauwen, Tanneke. "FtsW activity and lipid II synthesis are required for recruitment of MurJ to midcell during cell division in Escherichia coli". Molecular Microbiology 109 6 (2018): 855-884. http://dx.doi.org/10.1111/mmi.14104.
    10.1111/mmi.14104
  7. Meiresonne, Nils. "Detection of Protein Interactions in the Cytoplasm and Periplasm of Escherichia coli by Förster Resonance Energy Transfer". BIO-PROTOCOL 8 2 (2018): https://doi.org/10.21769%2Fbioprotoc.2697.
    10.21769/bioprotoc.2697
  8. Nils Y. Meiresonne; René van der Ploeg; Mark A. Hink; Tanneke den Blaauwen; Lotte Søgaard-Andersen. "Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Förster Resonance Energy Transfer Assay in Escherichia coli". mBio 8 5 (2017): https://doi.org/10.1128/mBio.01089-17.
    10.1128/mBio.01089-17
Online resource
  1. Meiresonne, Nils. Novel biophysical assay reveals that bacterial cell wall maintaining proteins change interaction conformation based on activity (Layman Summary). 2018. https://atlasofscience.org/novel-biophysical-assay-reveals-that-bacterial-cell-wall-maintaining-proteins-change-interaction-conformation-based-on-activity/.
Thesis / Dissertation
  1. Meiresonne, Nils. "Illumination protein interactions in the periplasm". PhD, Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, 2019. https://hdl.handle.net/11245.1/f8508764-0eff-4b25-891f-a9e822701ee4.
  2. Meiresonne, Nils. "Molecular mechanisms of bacterial cell division". Master, Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, 2012.
  3. Meiresonne, Nils. "Selection for antibiotic resistance due to intracellular survival of staphylococci in biomaterial associated infection". Master, Academic Medical Centre, Department of Medical Microbiology, Research Bacteriology, 2012.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/02/15 Visualizing bacterial protein interactions by FLIM-FRET Scan Seminars
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica (Portugal)
2021/04/01 Illuminating protein interactions in the periplasm (Invited) Royal Dutch Society for Microbiology Spring Meeting 2021
Royal Dutch Society for Microbiology (Papendal, Netherlands)
2020/03/31 Timing the critical protein interactions that drive bacterial cell cycle progression (Invited, canceled) Royal Dutch Society for Microbiology Spring Meeting 2020
Royal Dutch Society for Microbiology (Papendal, Netherlands)
2018/10/01 Illuminating Protein interactions in the Periplasm (Selected) Dutch Biophysics 2018
NWO domain Exact and Natural Sciences (ENW), in collaboration with the Dutch Society for Microscopy (NVvM), the Association for Biophysics and Biomedical Technology (BioPM), and the Dutch Society for Cell Biology (DSCB). (Veldhoven, Netherlands)
2018/09/18 Detecting antibiotic target protein interactions by in vivo FRET (Invited) Biomolecular Research Course 2018
Hogeschool Utrecht (Utrecht, Netherlands)
2018/06/24 Illuminating protein interactions in the periplasm (Invited) Amsterdam Microbiology meeting
Free University of Amsterdam / University of Amsterdam (Amsterdam, Netherlands)
2017/05/11 Activity related conformational changes of penicillin binding proteins (Invited) Swammerdam Institute of Life Sciences Seminar
Swammerdam Institute of Life Sciences, University of Amsterdam (Amsterdam, Netherlands)
2016/01/13 Illuminating periplasmic protein interactions (Invited) University of Amsterdam Microbiology Cluster Meeting
University of Amsterdam (Amsterdam, Netherlands)
2015/04/15 Periplasmic FRET (Invited) Royal Dutch Society for Microbiology Spring Meeting 2015
Royal Dutch Society for Microbiology (Arnhem, Netherlands)
2014/10/03 Measuring In vivo protein interactions of E. coli D,D-Carboxypeptidases by FRET 2-day conference exchange between the Laboratories of Waldemar Vollmer, Nassos Typas and Tanneke den Blaauwen of Newcastle University, the European Molecular Biology Laboratory and the University of Amsterdam, respectively.
University of Amsterdam (Amsterdam, Netherlands)
2014/05/15 Illuminating the periplasm: finding the right fluorophore (Invited) Royal Dutch Academy of Sciences 50 Years of Fts: the A-Z of Bacterial Cell Division
Royal Dutch Academy of Sciences
2014/03/07 ZapA: a link between cell division and chromosome segregation (Invited) Dutch Bacterial Cell Surfaces Meeting 2014
NWO (Amsterdam, Netherlands)

Event participation

Activity description
Type of event
Event name
Institution / Organization
2024/02/14 - 2024/03/27 Best practices in doctoral supervision, professional development of teaching and leadership skills for doctoral supervisors
Workshop
Best practices in doctoral supervision, professional development of teaching and leadership skills for doctoral supervisors
Karolinska Institutet, Sweden
2022/12/11 - 2022/12/15 Bacterial cell biophysics: DNA replication, growth, division, size and shape, Ein Gedi, Israel
Conference
Bacterial cell biophysics: DNA replication, growth, division, size and shape, Ein Gedi, Israel
European Molecular Biology Organization, Germany
2022/01/25 - 2022/01/26 Rosetrees Interdisciplinary workshop: Quantitative approaches to antimicrobial resistance and microbiology
Workshop
Rosetrees Interdisciplinary workshop: Quantitative approaches to antimicrobial resistance and microbiology
Physics of Life network, United Kingdom
2020/05 - 2022 Virtual S. aureus Seminar Series, monthly online seminar series covering Staphylococcus aureus
Seminar
Virtual S. aureus Seminar Series
New York University Long Island School of Medicine, United States
2020/06 - 2021/12 Cell Size and Growth seminar, bi-monthly online seminar series for and by the Cell Size research community.
Seminar
Cell Size and Growth seminar
Stanford University Department of Biology, United States
2020/11/23 - 2020/11/27 Beginners Guide to Fluorescence Lifetime Imaging (FLIM) in Life Sciences (online)
Conference
Beginners Guide to Fluorescence Lifetime Imaging (FLIM) in Life Sciences
CMCB light microscopy facility, Technische Universität Dresden, Germany
2020/10/21 - 2020/11/06 CauloConference 2.0 (Online)
Conference
CauloConference 2.0
Howard Hughes Medical Institute - Stanford University, United States
2020/02/21 - 2020/02/21 21st SymPhoTime Training Day
Workshop
21st SymPhoTime Training Day
PicoQuant GmbH, Germany
2020/02/18 - 2020/02/20 12th International Course on Time-resolved Microscopy and Correlation Spectroscopy
Workshop
12th International Course on “Time-resolved Microscopy and Correlation Spectroscopy
PicoQuant GmbH, Germany
2019/11/13 - 2019/11/15 Batch analysis and macro development in ImageJ/Fiji: Going beyond the basics: Short course on batch analysis of microscopy images.
Workshop
Batch analysis and macro development in ImageJ/Fiji: Going beyond the basics
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/11/04 - 2019/11/07 17th European Short Course on Principles and Applications of Time-resolved Fluorescence Spectroscopy
Workshop
17th European Short Course on “Principles and Applications of Time-resolved Fluorescence Spectroscopy
PicoQuant GmbH, Germany
2018/02 - 2018/02 Dutch Bacterial Cell Surfaces Meeting 2018
Conference
Dutch Bacterial Cell Surfaces Meeting 2018
Universiteit Utrecht, Netherlands
2017/02 - 2017/02 Dutch Bacterial Cell Surfaces Meeting 2017
Conference
Dutch Bacterial Cell Surfaces Meeting 2017
Rijksuniversiteit Groningen, Netherlands
2016/07/01 - 2016/07/14 UvA Summer School: Grant Writing and Proposal Development
Workshop
UvA Summer School: Grant Writing and Proposal Development
Universiteit van Amsterdam, Netherlands
2016/02 - 2016/02 Dutch Bacterial Cell Surfaces Meeting 2016
Conference
Dutch Bacterial Cell Surfaces Meeting 2016
Vrije Universiteit Amsterdam, Netherlands
2015/02 - 2015/02 Dutch Bacterial Cell Surfaces Meeting 2015
Conference
Dutch Bacterial Cell Surfaces Meeting 2015
Universiteit Leiden, Netherlands
2014/03 - 2014/03 Royal Netherlands Society for Microbiology: General and Molecular Microbiology 2014
Conference
General and Molecular Microbiology
Koninklijke Nederlands Vereniging voor Microbiologie, Netherlands
2014 - 2014 Antoni van Leeuwenhoek Centre of Advanced microscopy: Basics in Fluorescence Microscopy 2014
Workshop
Basics in Fluorescence Microscopy
Antoni van Leeuwenhoek Centre of Advanced microscopy, Netherlands
2013/03 - 2013/03 Dutch Bacterial Cell Surfaces Meeting 2013
Conference
Dutch Bacterial Cell Surfaces Meeting 2013
Rijksuniversiteit Groningen, Netherlands
2013 - 2013 Antoni van Leeuwenhoek Centre of Advanced microscopy: Confocal Microscopy Fundamentals, Advanced techniques and Biological Applications 2013
Workshop
Confocal Microscopy Fundamentals, Advanced techniques and Biological Applications
Antoni van Leeuwenhoek Centre of Advanced microscopy, Netherlands

Ad Hoc journal article review

Journal title (ISSN) Publisher
2023 - 2024 Frontiers in Bioengineering and Biotechnology (2296-4185) Frontiers Media
2023 - 2023 Molecular Microbiology (365-2958) Wiley-Blackwell
2023 - 2023 OpenMicrobiology (2045-8827) Wiley-Blackwell
2022 - 2022 Molecular Microbiology (1365-2958) Wiley-Blackwell
2022 - 2022 Frontiers in Microbiology (1664-302X) Frontiers Media
2021 - 2022 Nature Communications (2041-1723) Springer Nature
2017 - 2017 Journal of Bacteriology (1098-5530) ASM Journals

Association member

Society Organization name Role
2013/10/06 - Current Koninklijke Nederlandse vereniging voor microbiologie (Royal Netherlands Society for Microbiology) Member
2011/01/01 - Current Nederlands Instituut voor Biologie (Dutch institute for Biology) Member

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2023 - Current Advanced Microscopy Cell structures and functions (Master) Vrije Universiteit Amsterdam, Netherlands
2023 - Current Applications of FLIM to microbiology Fundamentals of light microscopy and image processing (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021 - 2021 Fluorescence techniques in bacteria Virtual Exchange activity between PSU, ITQB-NOVA and UvA (Bachelor) Prince of Songkla University, Thailand
2015 - 2019 Co-authored and assisted the practical training program of the course “Microbes and Man” for first year students of Biology, Biomedical Sciences and Psychobiology of the University of Amsterdam. Microbes and Man (Bachelor) Universiteit van Amsterdam, Netherlands

Mentoring / Tutoring

Topic Student name
2013 - 2019 Introduced three new PhD students and two Post Docs to the lab and specifically trained them in doing FRET experiments and analysis. 3x PhD 2x Postdoc
2013 - 2018 Supervised seven bachelor students' internships and one master student internship at the Bacterial Cell Biology group of the University of Amsterdam for periods between 3 and 6 months. 7x Bachelor and 1x Master
Distinctions

Award

2022 FEMS Meeting Attendance Grant (400) FEMS-MAG-2022-1,510
Federation Of European Microbiological Societies, Netherlands
2022 EMBO Travel grant (400) (w20-09-id81)
European Molecular Biology Organization, Germany
2019 Research and Training Grant (€3.200) - (FEMS-GO-2018-214.R1)
Federation Of European Microbiological Societies, Netherlands

Other distinction

2023 Yellow Flame for a popular plasmid deposited at the Addgene repository
Addgene Inc, United States
2020 Top Downloaded Paper 2018-2019
Wiley Publishing, United States
2016 3rd Prize Proposal “Novel Fluorescence Techniques for Antibiotic Development”
UvA summer school: Grant Writing and Proposal Development, Netherlands