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Luís Loureiro holds a MSc in Applied Biochemistry (2015) and a PhD (2022) by the Doctoral Program in Food Science and Technology and Nutrition from the University of Minho. During his PhD, he acquired technical competences and tools on extraction and processing technologies for microalgae biochemical compounds valorization. Namely, he acquires knowledge in cells disruption methodologies, biochemical compounds extraction and quantification and conducted an intensive optimization process of biological activity assays. His intellectual curiosity, as well as persistence and determination, led the LL to develop, since his graduation, a broad knowledge in different areas. Extensive experience in molecular biology techniques, namely genomics and proteomics, was obtained in his undergraduate internship at IPATIMUP. Innovative approaches in technologies such as Ohmic Heating (OH) and implementation of cell disruption techniques in the extraction of biochemical compounds in his PhD. Furthermore, the LL is currently developing a project called PROVEGG4PASTRY, in order to identify new sources of vegetable protein in place of animal protein for industrial pastry applications. This project allowed LL to develop functional and structural characterization studies of different vegetable proteins (pea, faba, chickpea and soy), as well as to develop preliminary studies of functionalization of proteins applying OH. Currently, LL is an assistant researcher in the Blue Bioeconomy Pact (BBP), a €133M consortium under the Recovery and Resilience Plan until 2025. The BBP includes 83 national entities: 53 companies and 30 ENESII organizations, including R&D centers, CoLABs, and universities. LL leads the implementation of electric field and OH technologies to process algae biomass into high-value extracts and ingredients, aiming to maximize biomass value and create new value chains. These valences have generated LL's interest in research that includes zero-waste and circular economy concepts, in order to take advantage of eco-innovate technologies to retrieve value from agro-industrial by-products and develop new food products.
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Identification

Personal identification

Full name
Luis Loureiro

Citation names

  • Loureiro, Luis

Author identifiers

Ciência ID
931B-6310-4540
ORCID iD
0000-0003-2091-5067

Knowledge fields

  • Engineering and Technology - Other Engineering and Technologies - Food and Beverages
  • Natural sciences - Biological Sciences - Biochemical Research Methods
  • Natural sciences - Biological Sciences - Biochemistry
  • Natural sciences - Biological Sciences - Microbiology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Intermediate (B1) Advanced (C1) Upper intermediate (B2) Upper intermediate (B2)
Spanish; Castilian Beginner (A1) Intermediate (B1) Intermediate (B1) Beginner (A1)
Education
Degree Classification
2022/12/22
Concluded
 Ciência e Tecnologia Alimentar e Nutrição (Doutoramento)
Universidade do Minho, Portugal
"Valorisation of extremophilic microalgae isolated from lichens: development of cultivation and extraction strategies for high performance production of bioactive compounds" (THESIS/DISSERTATION)
 Very Good
2015
Concluded
 Bioquímica Aplicada (Mestrado)
Universidade do Minho Escola de Ciências, Portugal
"Functional characterization of mitochondrial suspensions by cytofluorimetric techniques towards the screening of mitochondrial target drugs" (THESIS/DISSERTATION)
 17
2013
Concluded
 Host-Fungus Interactions and Virulence (Outros)
Universidade do Minho Escola de Ciências, Portugal
"n/a" (THESIS/DISSERTATION)
 17
2013
Concluded
 Nanoparticles and the immune system: risks and therapeutic opportunities (Outros)
Universidade do Minho Escola de Ciências, Portugal
"n/a" (THESIS/DISSERTATION)
 17
2012
Concluded
 Bioquímica (Licenciatura)
Universidade do Porto Faculdade de Ciências, Portugal
"Caracterização da região do gene cagA que codifica os motivos de fosforilação EPIYA em diferentes estirpes de Helicobacter pylori" (THESIS/DISSERTATION)
 15
2007
Concluded
 Ciências e Tecnologias (Ensino secundário)
Escola Secundária de Barcelos, Portugal
"n/a" (THESIS/DISSERTATION)
 18
Affiliation

Science

Category
Host institution 		Employer
2023/07/01 - 2025/12/31 Invited Auxiliary Researcher (Research) Universidade do Minho, Portugal
Universidade do Minho, Portugal
2022 - 2023/06/01 Researcher (Research) Universidade do Minho Centro de Engenharia Biológica, Portugal

Others

Category
Host institution 		Employer
2015/09/01 - 2016/03/01 Researcher in the International project: Multicompartment lipid nanocarriers as enhancers of therapeutic loading: towards optimized cancer nanotherapy Ref. 20155321 - Funded by Elettra Sincrotrone Tr Universidade do Minho, Portugal
2015/05/01 - 2015/08/30 Researcher in the project:“Development of high-throughput biophysical strategies for supporting the design of therapeutic nanocarriers with increased efficacy and reduced toxicity” Ref. IF/00498/2012/ Universidade do Minho, Portugal
2013/09/01 - 2015/04/01 Researcher under the subject: "Functional characterization of mitochondrial suspensions by cytofluorimetric techniques towards the screening of mitochondrial target drugs" - Master Thesis Universidade do Minho, Portugal
2014/12/17 - 2014/12/20 Participation in "XVIII Congresso da sociedade Bioquímica de Portugal" Sociedade Portuguesa de Bioquímica, Portugal
2014/11/27 - 2014/11/30 Participation in "II Symposium of the PhD Programme on Molecular and Environmental Biology" Universidade do Minho, Portugal
2013/06/01 - 2013/07/01 Participation in "Post-Graduated Training Course: Nanoparticles and the immune system: risks and therapeutic opportunities" Universidade do Minho, Portugal
2013/04/01 - 2013/05/01 Participation in "Post-Graduated Training Course: Host-Fungus Interactions and Virulence” Universidade do Minho, Portugal
2013/01/01 - 2013/03/01 Collaboration in class modules "Métodos de Análise Celular e Biomolecular" of the Applied Biochemistry Master Universidade do Minho, Portugal
2012/01/01 - 2012/07/01 Researcher under the subject: "Characterization of cagA gene region encoding the phosphorylation motifs EPIYA in different Helicobacter pylori strains" - Graduation Thesis Universidade do Minho, Portugal
2010/05/01 - 2010/05/03 Participation in congress “Biologia e Microbiologia do cancro” Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Projects

Grant

Designation Funders
2017/10/01 - 2022/01/01 Valorisation of extremophilic microalgae: development of cultivation and extraction strategies for high performance production of bioactive compounds
SFRH/BD/131410/2017
PhD Student Fellow

Contract

Designation Funders
2019/01/01 - Current Centre of Biological Engineering - University of Minho
UID/BIO/04469/2019
Universidade do Minho, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2023/07/01 - 2025/12/31 “Pacto da Bioeconomia Azul” - Projeto Vertical Algas
PRR/BioEconomiaAzul/WP5/CEB
Post-doc
Ongoing
2023/03/01 - 2024/08/31 Methane mitigation strategies based on photosynthetic microorganisms
2022.05196.PTDC
Universidade do Minho, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2023/07/01 - 2023/12/31 Plasmon-Enhanced Photosynthetic Microalgae Growth for CO2 Bio-Mitigation
EXPL/CTM-REF/0750/2021
10.54499/EXPL/CTM-REF/0750/2021
Universidade do Minho, Portugal

Universidade do Minho Centro de Engenharia Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2022 - Current CH4GREEN - Methane mitigation strategies based on photosynthetic microorganisms
2022.05196.PTDC
Researcher
2024/10/01 - 2026 TRANS-lighthouses -More than green - Lighthouses of transformative nature-based solutions for inclusive communities
101084628
Training director of the workshop "Nature and Sustainability in Nature" to be carried out within the scope of the Consortium Trans-Lighthouses.
Camara Municipal de Barcelos, Portugal
Ongoing
2022/06/02 - 2023/06/02 PROVEGG4PASTRY - Development of innovative formulations using vegetable protein sources in place of animal protein for industrial pastry applications
PROVEGG4PASTRY- NORTE-01-0247-FEDER-047177
Research Fellow
Ongoing
2016/09/01 - 2017/01/31 Valorisation of extremophilic microalgae: development of cultivation and extraction strategies for high performance production of bioactive compounds
UMinho/BI-Master/microalgas extremófilas/CEB_dez2016 Centre of Biological Engineering (CEB) - University of Minho
Colaborador
Concluded
2016/03/01 - 2016/06/01 Development of high-throughput biophysical strategies for supporting the design of therapeutic nanocarriers with increased efficacy and reduced toxicity
Ref. IF/00498/2012/CP0177/CT0005 University of Minho (Department of Physics)
Colaborador
2016/01/01 - 2016/06/01 Multicompartment lipid nanocarriers as enhancers of therapeutic loading: towards optimized cancer nanotherapy
Ref. 20155321 - Funded by Elettra Sincrotrone Trieste University of Minho (Department of Physics)
Colaborador
Outputs

Publications

Conference abstract
  1. Loureiro, Luis; Cristina M. R. Vicente; António Augusto Martins de Oliveira Soares Vicente; Pereira N. Ricardo; Teixeira, José A.. "Enzymatic Pretreatment for Enhanced Ohmic Heating Effect on Nannochloropsis gaditana Cells Disruption/Permeabilization". Paper presented in 10TH International conference on Food Chemistry & Technology, Valência, 2024.
  2. Vardanega, Renata; Lüdtke, Fernanda L.; Loureiro, Luis; Gonçalves, Raquel F.S.; Martins, Joana T.; Ana C. Pinheiro; Vicente, Antonio A.. Corresponding author: Vardanega, Renata. "Nanostructured Lipid Carriers for Enhanced Cannabidiol Bioaccessibility: In vitro Digestion Study and Cytotoxicity Assessment". Paper presented in 10TH International conference on Food Chemistry & Technology, Valência, 2024.
  3. Isaías, Ricardo; Ribeiro, José Carlos; Navarro, Pedro; Loureiro, Luis; Rocha, Célia; Vicente, António Augusto; Cunha, Luís Miguel. Corresponding author: Cunha, Luís Miguel. "Towards the formulation of insect-based foods: systematic review on insect protein’s impact on techno-functional properties". Paper presented in 38th EFFoST International Conference 2024, Brugge, 2024.
  4. Isaías, Ricardo; Loureiro, Luis; Ribeiro, José Carlos; Rocha, Célia; Vicente, António Augusto; Cunha, Luís Miguel. Corresponding author: Cunha, Luís Miguel. "Towards the Improvement of Techno-functional Properties: Effects of Mixing Alternative Proteins on Technological Performance". Paper presented in 17º Encontro de Química dos Alimentos, Vila Real, 2024.
  5. Vardanega, Renata; Lüdtke, Fernanda L.; Loureiro, Luis; Gonçalves, Raquel F.S.; Ana C. Pinheiro; Vicente, Antonio A.. "Nanostructured lipid carriers for simultaneous delivery of cannabidiol and the bioenhancer piperine". Paper presented in IUFOST 2024 - International Union of Food Science and Technology, 2024.
  6. Vardanega, Renata; Lüdtke, Fernanda Luisa; Loureiro, Luis; Gonçalves, Raquel; Vicente, António A.. "Nanostructures as conveyors of functionality in foods: The case of cannabidiol-based nanostructured lipid carriers". Paper presented in Food Chemistry & Technology, Paris, 2023.
  7. Vardanega, Renata; Lüdtke, Fernanda Luisa; Loureiro, Luis; Toledo Hijo, Ariel A. A.; Vicente, António A.. Corresponding author: Vardanega, Renata. "Nanostructured lipid carriers produced with ionic liquid as novel natural- derived emulsifier to enhance the bioavailability of cannabidiol". Paper presented in Delivery of Functionality in Complex Food Systems, 2023.
  8. Sousa, Vítor; Loureiro, Luis; Carvalho, Gonçalo; Pereira N. Ricardo. "Improving the extraction of biomolecules from Coelastrella sp biomass using Ohmic Heating". Paper presented in Ibero-American Congress on Biotechnology (07-04-2022), Braga, 2022.
    Accepted
Conference poster
  1. Isaías, R. N.; Loureiro, Luis; Ribeiro, José Carlos; Rocha, Célia; Cunha, Luís Miguel; Vicente, A.A.. "Effects of Mixing Alternative Proteins on Technological Performance". Paper presented in V CBEDS - 5th Chemical and Biological Engineering Doctoral Symposium, 2024.
  2. Loureiro, Luis; Vardanega, Renata; António Augusto Martins de Oliveira Soares Vicente; Pereira N. Ricardo. Corresponding author: Loureiro, Luis. "Assessment of the ohmic heating effect on the extraction of bioactive compounds from Cannabis sativa L.". Paper presented in IUFOST 2024 - International Union of Food Science and Technology, 2024.
  3. Walter de Paula Pinto Neto; Márcia Cristina Teixeira Marques; Raquel F. S. Gonçalves; Loureiro, Luis; Ana Cristina Pinheiro; António Augusto Vicente; Rafael Barros de Souza; Marcos Antonio de Morais Junior. Corresponding author: Walter de Paula Pinto Neto. "Utilização de diferentes méis de abelhas do Bioma Caatinga (Brasil) sobre a sobrevivência de Saccharomyces boulardii CNCM I-745 sob simulação in vitro do trato gastrointestinal". Paper presented in Ciência 2024 - Science and Technology Meeting in Portugal, 2024.
  4. Isaías, Ricardo; Loureiro, Luis; Ribeiro, José Carlos; Cunha, Luís Miguel; Vicente, António Augusto. Corresponding author: Isaías, Ricardo. "Techno-functional characterisation of several pulse protein sources for food applications". Paper presented in Ciência 2024 - Science and Technology Meeting in Portugal, 2024.
  5. Rodrigues, Rui M.; Miguel Azevedo; Beatriz Silva; Leite, Ana Catarina; Loureiro, Luis; Pereira N. Ricardo; António Vicente. Corresponding author: Rodrigues, Rui M.. "PROVEGG4PASTRY Development of innovative bakery preparations using vegetable protein sources to replace animal proteins in industrial bakery applications". Paper presented in Dare2CHange, 2023.
  6. Ana Catarina Leite; Loureiro, Luis. Corresponding author: Ana Catarina Leite. "Functional and physicochemical characterization of emerging plant-based proteins". Paper presented in IX International Conference on Food Proteins and Colloids, 2023.
  7. Ana Catarina Leite; Loureiro, Luis. Corresponding author: Loureiro, Luis. "Improvement of the functional properties of pea and faba proteins for pastry applications". Paper presented in IX International Conference on Food Proteins and Colloids, 2023.
  8. Sousa, Vítor; Loureiro, Luis; Pereira N. Ricardo. "Extraction bioactive molecules from Coelastrella sp. LFR1 biomass using ohmic heating". Paper presented in XVI Encontro de Química dos Alimentos, 2022.
  9. Loureiro, Luis. "Cultivation Systems of Coelastrella sp. isolated from lichens collected in the north of Portugal". Paper presented in BioIberoAmerica 2022, 2022.
  10. Sousa, Vítor; Loureiro, Luis; Vicente, A.A.; Pereira N. Ricardo. "Ohmic Heating: a sustainable way to extract bioactive compounds from Coelastrella spp biomass". Paper presented in CBEDS - Chemical and Biological Engineering Doctoral Symposium, 2021.
  11. Luís Pedro Lima Machado; Loureiro, Luis. "Cellular disruption induced by ultrasonic waves in microalgae isolated from lichens". Paper presented in Jornadas da Ciência, 2019, 2019.
  12. Loureiro, Luis. "Valorization of extremophilic microalgae isolated from lichens". Paper presented in Conferência da Associação Portuguesa de Algologia Aplicada (APAA) 2019, 2019.
  13. Anna Matejcíková; Loureiro, Luis. Corresponding author: Anna Matejcíková. "Development of cultivation Strategies for Photobionts Isolated from Lichens". 2019.
  14. Geada, Pedro; Loureiro, Luis. Corresponding author: Geada, Pedro. "Comparison of harvesting methods for the cyanobacterium Microcystis aeruginosa". Paper presented in BioTech 2017 & the 7th Czech-Swiss Symposium, 2017.
  15. Geada, Pedro; Loureiro, Luis. Corresponding author: Geada, Pedro. "Strategy development to optimize the production of a high added-value cyanotoxin". Paper presented in 44th International Conference of the Slovak Society of Chemical Engineering, 2017.
  16. Geada, Pedro; Loureiro, Luis. Corresponding author: Geada, Pedro. "Assessment of cell disruption efficiency of Microcystis aeruginosa using different mechanical techniques". Paper presented in 44th International Conference of the Slovak Society of Chemical Engineering, 2017.
Journal article
  1. Vardanega, Renata; Lüdtke, Fernanda L.; Loureiro, Luís; Toledo Hijo, Ariel A.C.; Martins, Joana T.; Pinheiro, Ana C.; Vicente, António A.. "Enhancing cannabidiol bioaccessibility using ionic liquid as emulsifier to produce nanosystems: Characterization of structures, cytotoxicity assessment, and in vitro digestion". Food Research International 188 (2024): 114498. http://dx.doi.org/10.1016/j.foodres.2024.114498.
    10.1016/j.foodres.2024.114498
  2. Loureiro, Luís; Morais, João; Silva, Raquel; Martins, Joana T.; Geada, Pedro; Vasconcelos, Vítor; Vicente, António A.. "Isolation and Identification of Lichen Photobionts Collected from Different Environments in North of Portugal and Evaluation of Bioactivities of Their Extracts". Foods 13 11 (2024): 1759. http://dx.doi.org/10.3390/foods13111759.
    10.3390/foods13111759
  3. Vardanega, Renata; Lüdtke, Fernanda L.; Loureiro, Luís; Gonçalves, Raquel F.S.; Pinheiro, Ana C.; Vicente, António A.. "Development and characterization of nanostructured lipid carriers for cannabidiol delivery". Food Chemistry 441 (2024): 138295. http://dx.doi.org/10.1016/j.foodchem.2023.138295.
    10.1016/j.foodchem.2023.138295
  4. Loureiro, Luis; Machado, Luis; Geada, Pedro; Vasconcelos, Vitor; Vicente, Antonio A.. "Evaluation of efficiency of disruption methods for Coelastrella sp. in order to obtain high yields of biochemical compounds release". Algal Research (2023): https://publons.com/wos-op/publon/61642337/.
    10.1016/J.ALGAL.2023.103158
  5. Sousa, V.; Loureiro, L.; Carvalho, G.; Pereira, R.N.. "Extraction of biomolecules from Coelastrella sp. LRF1 biomass using Ohmic Heating technology". Innovative Food Science & Emerging Technologies 80 (2022): 103059. http://dx.doi.org/10.1016/j.ifset.2022.103059.
    10.1016/j.ifset.2022.103059
  6. Sousa, Vítor; Loureiro, Luis; Carvalho, Gonçalo; Pereira N. Ricardo. "Extraction of biomolecules from Coelastrella sp. biomass using high-temperature short time pre-treatment through Ohmic Heating technology". Innovative Food Science and Emerging Technologies (2022):
    Under revision
  7. Geada, Pedro; Loureiro, Luís; Teixeira, José A.; Vasconcelos, Vítor; Vicente, António A.; Fernandes, Bruno D.. "Evaluation of disruption/permeabilization methodologies for Microcystis aeruginosa as alternatives to obtain high yields of microcystin release". Algal Research 42 (2019): 101611. http://dx.doi.org/10.1016/j.algal.2019.101611.
    10.1016/j.algal.2019.101611
  8. Geada, Pedro; Oliveira, Francisca; Loureiro, Luís; Esteves, Diogo; Teixeira, José A.; Vasconcelos, Vítor; Vicente, António A.; Fernandes, Bruno D.. "Comparison and optimization of different methods for Microcystis aeruginosas harvesting and the role of zeta potential on its efficiency". Environmental Science and Pollution Research 26 16 (2019): 16708-16715. http://dx.doi.org/10.1007/s11356-019-04803-0.
    10.1007/s11356-019-04803-0
  9. Geada, Pedro; Rodrigues, Rui; Loureiro, Luís; Pereira, Ricardo; Fernandes, Bruno; Teixeira, José A.; Vasconcelos, Vítor; Vicente, António A.. "Electrotechnologies applied to microalgal biotechnology - Applications, techniques and future trends". Renewable and Sustainable Energy Reviews 94 (2018): 656-668. http://dx.doi.org/10.1016/j.rser.2018.06.059.
    10.1016/j.rser.2018.06.059
  10. Soares, Telma Bezerra; Loureiro, Luís; Carvalho, Ana; Oliveira, Maria Elisabete C.D. Real; Dias, Alberto; Sarmento, Bruno; Lúcio, Marlene. "Lipid nanocarriers loaded with natural compounds: Potential new therapies for age related neurodegenerative diseases?". Progress in Neurobiology 168 (2018): 21-41. http://dx.doi.org/10.1016/j.pneurobio.2018.04.004.
    10.1016/j.pneurobio.2018.04.004
  11. Pereira, Cátia S.; Guedes, Joana P.; Gonçalves, Marília; Loureiro, Luís; Castro, Lisandra; Gerós, Hernâni; Rodrigues, Lígia R.; Côrte-Real, Manuela. "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase". Oncotarget 7 38 (2016): 62144-62158. http://dx.doi.org/10.18632/oncotarget.11394.
    10.18632/oncotarget.11394
Preprint
  1. Vardanega, Renata; Lüdtke, Fernanda Luisa; Loureiro, Luis; Gonçalves, Raquel; Pinheiro, Ana Cristina; Vicente, António Augusto. "Food Grade Nanostructured Lipid Carriers as an Oral Delivery System of Cannabidiol: Development and Characterization". 2023. http://dx.doi.org/10.2139/ssrn.4634716.
    10.2139/ssrn.4634716
Thesis / Dissertation
  1. "Valorisation of extremophilic microalgae: development of cultivation and extraction strategies for high performance production of bioactive compounds". PhD, 2022.
  2. "Functional characterization of mitochondrial suspensions by cytofluorimetric techniques towards the screening of mitochondrial target drugs". Master, 2015.
  3. "Characterization of cagA gene region encoding the phosphorylation motifs EPIYA in different Helicobacter pylori strains". Degree, Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, 2012.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2017/06/04 Assessment of cell disruption efficiency of Microcystis aeruginosa using different mechanical techniques
2016/11/09 Valorisation of extremophilic microalgae: development of cultivation and extraction strategies for high performance production of bioactive compounds; upstream and downstream processing
2015/05/03 Functional characterization of mitochondrial suspensions by cytofluorimetric techniques towards the screening of mitochondrial target drugs

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2023/12/06 Effect of different cultivation conditions of photosynthetic microorganisms on methane consumption
(Thesis) Arguer
 Ana Sofia Maciel Soares (Master)
Universidade do Minho, Portugal

Mentoring / Tutoring

Topic Student name
2023 - 2027 Development, characterization and sensory analysis of protein mixtures from insects and legumes Ricardo Isaías
2019 - 2020 Evaluation of Ohmic Heating processing on extraction of bioactive compounds from the microalgae biomass Vítor Sousa
2019 - 2019 Development of Cultivation Systems and strategies for Photobionts isolated from lichens Anna Matejcíková
2018 - 2019 Ohmic heating, an extraction method for lichen isolated microalgaes bioactive compounds João Fonseca
2018 - 2019 Cell disruption strategies for extraction of bioactive compounds from lichens photobionts Luís Pedro Lima Machado
2017 - 2018 Screening for antioxidant activity in extracts obtained from extremophile microalgae Carla Machado de Sousa
Distinctions

Other distinction

2016 Participation award (1 of 2 Portuguese researchers selected and funded) for the 1st training School - Microalgae based processes: upstream and downstream