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Elisabete Fernandes is an assay development scientist specializing in biosensors. She is a staff researcher in the Nanodevices Group at the International Iberian Nanotechnology Laboratory (INL). Her responsibilities include developing assay formats, surface functionalization, and applying biosensors for medical biomarkers detection. She is also actively involved in the development of the research strategy of the health cluster at INL (as deputy coordinator), and also responsible for the Clinical laboratory at INL, overseeing its implementation (BSL2). Elisabete received her Ph.D. degree in Chemical and Biological Engineering from the University of Minho, Portugal, in 2013. Her doctoral research focused on developing a biosensor using bacteriophages as biorecognition elements, with part of the work conducted at the University of Auburn, USA, and the University of Leuven, Belgium. During her Ph.D., she received a national grant (FCT), and then a Marie Curie Fellowship for her participation in an EU-funded project related to cancer diagnosis. Elisabete has 16 peer-reviewed papers in scientific journals related to biosensors, 1 book chapter in an international scientific book, presented her research through 28 oral communications and 18 posters at various international scientific seminars, workshops, and conferences. Elisabete is currently co-supervising 1 Ph.D. student and 1 Master's student. Previously, she has supervised 1 Postdoc and co-supervised a Ph.D. student (successfully defended in 2022) in the biosensor field applied to stroke, along with 4 Master's. Elisabete has served as project coordinator of Fully Integrated Magnetoresistive Biochip Platform for Stroke Patient Stratification (FIM4Stroke), funded through a national program (FCT, 250k). She has actively participated in several national (5) and international (6) projects. Since 2014, Elisabete has dedicated her primary attention to advancing the development of a Point-of-Care (PoC) technology, specifically focusing on the utilization of Magnetoresistive platforms and the creation of innovative detection strategies for stroke. Beyond her scientific pursuits, Elisabete is a multidisciplinary researcher actively involved in pedagogical projects at national and international levels. Her work has been published in science communication and pedagogical practice conferences. She is also a passionate advocate for knowledge humanization and accessibility. Her commitment to these ideals is evident in her affiliations and engagements. She is proudly associated with National Geographic as a certified educator, actively contributing to the promotion of scientific literacy. Furthermore, Elisabete holds the role of co-founder and leader of Mission NERD, a volunteer project that aims to foster scientific literacy and break stereotypes at INL. In addition to her educational endeavors, Elisabete's dedication to compassion and altruism is exemplified through her certification as an educator and ambassador of applied compassion from Stanford University's Center for Compassion and Altruism Research and Education (CCARE). Through this program, she actively promotes and embodies the principles of compassion in her work and interactions, further enriching her contributions to society.
Identification

Personal identification

Full name
Elisabete Fernandes

Citation names

  • Fernandes, Elisabete

Author identifiers

Ciência ID
8B11-15E7-4F5D
ORCID iD
0000-0003-4558-7047
Researcher Id
B-8509-2011

Email addresses

  • elisabete.fernandes@inl.int (Professional)

Addresses

  • International Iberian Nanotechnology Laboratory - INL. Avenida Mestre José Veiga s/n, 4715-330, Braga, Braga, Portugal (Professional)

Websites

Knowledge fields

  • Engineering and Technology - Nano-technology
  • Social Sciences - Educational Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Intermediate (B1) Advanced (C1)
Spanish; Castilian Intermediate (B1) Advanced (C1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2021 - 2022
Concluded
Educator and Ambassador of Applied Compassion, (Outros)
Stanford University, United States
2020
Concluded
Certified National Geographic Educator (Outros)
National Geographic Society, United States
2013
Concluded
PhD in Chemical and Biological Engineering (Doutoramento)
Universidade do Minho Escola de Engenharia, Portugal
"Development of a phage-based biosensor to detect Salmonella in food stuff." (THESIS/DISSERTATION)
Doctor
2007
Concluded
Biological Engineering (Mestrado integrado)
Universidade do Minho Escola de Engenharia, Portugal
"Bactiophages for Salmonella control: Comparison of different methods of preservation" (THESIS/DISSERTATION)
15
Affiliation

Science

Category
Host institution
Employer
2016/12/09 - Current Contracted Researcher (Research) International Iberian Nanotechnology Laboratory, Portugal
2014/06/01 - 2016/12/08 Researcher (Research) International Iberian Nanotechnology Laboratory, Portugal
2013/06/01 - 2014/06/30 Contracted Researcher (Research) Xeptagen SpA, Italy
École Polytechnique Fédérale de Lausanne, Switzerland

Others

Category
Host institution
Employer
2020/12/08 - Current Certified Educator of the National Geographic n/a, United States
Projects

Grant

Designation Funders
2012/01/01 - 2014/06/01 PROSENSE (Parallel Sensing of Prostate Cancer Biomarkers)
PROSENSE
Post-doc Fellow
Concluded

Contract

Designation Funders
2018/10/04 - Current FIM4Stroke - A Fully Integrated Magnetoresistive Biochip Platform for Stroke Patient Stratification .
PTDC/MEC-URG/29561/2017
Principal investigator
Fundação para a Ciência e a Tecnologia
Ongoing
2017/09/01 - Current Occult Hepatitis C in Different Clinical Settings: Detection, Characterization and Diagnostic Tools
PTDC/SAU-SER/30788/2017
Researcher
Fundação para a Ciência e a Tecnologia
2017/07/01 - Current NanoEaters – Early Detection of Peritoneal Fibrosis
0181_NANOEATERS_1_E
Researcher
2016/07/01 - Current NanoEaters – Biomarkers for early diagnosis of colorectal cancer
0181_NANOEATERS_1_E
Researcher
Ongoing
2018/10/01 - 2021/09/30 Infeção Oculta pelo Vírus da Hepatite C em diferentes abordagens clínicas: Deteção, Caracterização e Ferramentas de Diagnóstico
PTDC/SAU-SER/30788/2017
Centro Hospitalar e Universitário de Coimbra EPE, Portugal

International Iberian Nanotechnology Laboratory, Portugal

INESC Microsistemas e Nanotecnologias, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2015/01/01 - 2016 InveNNta 2 - Innovation in Nanomedicine: Design of Nanostructures for the Treatment and Diagnosis of Cancer and Stroke
InveNNta 2
Researcher
Concluded
2013/09/01 - 2015 InveNNta 1 - Innovation in Nanomedicine: Design of Nanostructures for the Treatment and Diagnosis of Cancer and Stroke
InveNNta 1
Researcher
Concluded
2007/09/01 - 2008 European Project Phagevet-P (Veterinary phage therapies as alternatives to antibiotics in poultry production, STREP Project no. 2005-7224)
STREP Project no. 2005-7224
Scientific Initiation Fellow
Concluded

Other

Designation Funders
2017/09/01 - 2019 Hackaton 2017 - On-chip selection of exosomal vectors for neurodegenerative diseases treatment
INL&UMinho
Researcher
Concluded
Outputs

Publications

Conference poster
  1. Cabral, Patrícia; Rodrigues, Teresa; Borme, Jérôme; Fernandes, Elisabete; Alpuim, Pedro. "Field-Effect Transistors as biosensors: building up for the end application". Paper presented in Jornadas CF-UP-UP, 2019.
  2. Fernandes, Elisabete; Freitas, Paulo P.; Cabral, Patrícia; Rodrigues, Teresa; Alpuim, Pedro. "Comparison of magnetoresistive and graphene based platforms for the detection of clinical biomarkers". Paper presented in INL Annual Research Symposium 2019, 2019.
  3. Alpuim, Pedro; Machado Jr., George; Cabral, Patrícia; Fernandes, Elisabete; Campos, Rui; Borme, Jérôme. "Graphene-based devices for bio-sensing platforms". Paper presented in Nanolito 2017, 2017.
  4. Cabral, Patrícia D.; Fernandes, Elisabete; Cerqueira, M. Fátima; Machado Jr., George; Borme, Jérôme; Alpuim, Pedro. "Graphene Functionalization for Biosensing". Paper presented in 1st iBEM – international Biomedical Engineering Meeting,, 2016.
Journal article
  1. Andrea Cruz; Elisabete Fernandes; Raquel O. Rodrigues; Susana O. Catarino; Diana Pinho. "Editorial: Disease-on-a-chip: from point-of-care to personalized medicine". Frontiers in Pharmacology (2023): http://dx.doi.org/10.3389/fphar.2023.1344379.
    10.3389/fphar.2023.1344379
  2. Briliant Adhi Prabowo; Carole Sousa; Susana Cardoso; Paulo Freitas; Elisabete Fernandes. "Labeling on a Chip of Cellular Fibronectin and Matrix Metallopeptidase-9 in Human Serum". Micromachines (2022): https://doi.org/10.3390/mi13101722.
    10.3390/mi13101722
  3. D. C. Albuquerque; Verónica C. Martins; Elisabete Fernandes; Líbia Zé-Zé; Maria João Alves; Susana Cardoso. "Combined detection of molecular and serological signatures of viral infections: The dual assay concept". Biosensors and Bioelectronics (2022): http://dx.doi.org/10.1016/j.bios.2022.114302.
    10.1016/j.bios.2022.114302
  4. Briliant Adhi Prabowo; Elisabete Fernandes; Paulo Freitas. "A pump-free microfluidic device for fast magnetic labeling of ischemic stroke biomarkers". Analytical and Bioanalytical Chemistry 414 8 (2022): 2571-2583. https://doi.org/10.1007/s00216-022-03915-w.
    10.1007/s00216-022-03915-w
  5. "The Challenges of Developing Biosensors for Clinical Assessment: A Review". Chemosensors 2021 (2021):
    https://doi.org/10.3390/chemosensors9110299
  6. Cabral, Patrícia D.; Domingues, Telma; Machado, George; Chicharo, Alexandre; Cerqueira, Fátima; Fernandes, Elisabete; Athayde, Emília; Alpuim, Pedro; Borme, Jérôme. "Clean-Room Lithographical Processes for the Fabrication of Graphene Biosensors". Materials 13 24 (2020): 5728. http://dx.doi.org/10.3390/ma13245728.
    10.3390/ma13245728
  7. Fernandes, Elisabete; Sobrino, Tomás; Martins, Verónica C.; Lopez-loureiro, Ignacio; Campos, Francisco; Germano, José; Rodríguez-Pérez, Manuel; et al. "Point-of-care quantification of serum cellular fibronectin levels for stratification of ischemic stroke patients". Nanomedicine: Nanotechnology, Biology and Medicine 30 (2020): 102287. http://dx.doi.org/10.1016/j.nano.2020.102287.
    10.1016/j.nano.2020.102287
  8. Fernandes, Elisabete. "Functionalization of single-layer graphene for immunoassays". Applied Surface Science (2019): http://dx.doi.org/10.1016/j.apsusc.2019.03.004.
    10.1016/j.apsusc.2019.03.004
  9. Fernandes, Elisabete. "Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection". Biosensors and Bioelectronics (2018): http://dx.doi.org/10.1016/j.bios.2017.09.004.
    10.1016/j.bios.2017.09.004
  10. Fernandes, Elisabete. "Adapting Bobbert-Vlieger model to spectroscopic ellipsometry of gold nanoparticles with bio-organic shells". Biomedical Optics Express (2017): http://dx.doi.org/10.1364/boe.8.003538.
    10.1364/boe.8.003538
  11. Fernandes, Elisabete. "Rapid and specific detection of cell-derived microvesicles using a magnetoresistive biochip". Analyst (2017):
    10.1039/c6an02651f
  12. Dias, T.M.; Fernandes, E.; Cardoso, S.; Monteiro, G.; Freitas, P.P.. "One-step trapping of droplets and surface functionalization of sensors using gold-patterned structures for multiplexing in biochips". RSC Advances 7 68 (2017): 43273-43282. http://www.scopus.com/inward/record.url?eid=2-s2.0-85029126517&partnerID=MN8TOARS.
    10.1039/c7ra06085h
  13. Freitas, Paulo P.; Martins, Veronica C.; Cardoso, Felipe A.; Fernandes, Elisabete; Sobrino, Tomas; Castillo, Jose; Chicharo, Alexandre; et al. "Spintronic Biochips". Nanomagnetism: Applications and Perspectives (2017): https://doi.org/10.1002/9783527698509.ch9.
    doi:10.1002/9783527698509.ch910.1002/9783527698509.ch9
  14. Fernandes, Elisabete. "A bacteriophage detection tool for viability assessment of Salmonella cells". Biosensors and Bioelectronics (2014): http://dx.doi.org/10.1016/j.bios.2013.08.053.
    10.1016/j.bios.2013.08.053
  15. Fernandes, Elisabete. "Selection and Characterization of a Multivalent Salmonella Phage and Its Production in a Nonpathogenic Escherichia coli Strain". Applied and Environmental Microbiology (2010): http://dx.doi.org/10.1128/aem.00922-10.
    10.1128/aem.00922-10
  16. Fernandes, Elisabete. "Method for bacteriophage isolation against targetCampylobacterstrains". Letters in Applied Microbiology (2010): http://dx.doi.org/10.1111/j.1472-765x.2009.02774.x.
    10.1111/j.1472-765x.2009.02774.x
Activities

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2023/12/01 - 2025/04/07 Shelf-life optimization of antibody-coated sensor surfaces for PoC applications
Supervisor
Master in Biomedical Engineering (Master)
Universidade do Minho, Portugal
2018/01/01 - 2025/01/09 Self-sufficient Point-of-care Platform for Diagnosis of Tropical Diseases
Co-supervisor
Universidade Nova de Lisboa, Portugal
2017/01/01 - 2022/01/05 Immuno-field-effect transistor platforms based on 2D materials for early detection of biomarkers of ischemic stroke
Co-supervisor
Universidade do Minho, Portugal
2017/01/01 - 2022/01/04 MicroPathID –Rapid in-field microfluidic system for diagnostics of pathogens
Co-supervisor
Universidade Nova de Lisboa, Portugal
2016 - 2017 Characterization of cell-derived microparticles by nanotechnological methods,
Co-supervisor
Danmarks Tekniske Universitet, Denmark

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2024/03/21 LOBOWARNING – Mitigation of Globodera spp. outbreaks in Portugal through an innovative early nano-detection system and biocontrol Maria João Santiago Militão Camacho (PhD)
2022/05/18 Microfluidic biosensor for antibody therapy management Inês Raquel da Silva Iria (PhD)
2022/01/19 Graphene field-effect transistors functionalization for neurotransmitter18 / 27 biosensing
(Thesis) Main arguer
Mafalda Abrantes (Master)
2021/01/19 Development of a microfluidic sample preparation system for bacteria magnetic labelling and capture in clinical samples
(Thesis) Main arguer
Davide Carta (Master)
2017/12/07 Prototyping a Spintronics Labon-Chip system for cancer liquid biopsy analysis
(Thesis) Main arguer
Tomás Miguel de Freitas Dias (PhD)
2016/10/28 Studying DNA compaction by the Condensin complex using magnetic tweezers
(Thesis) Main arguer
Ana Carolina Faustino Mota (Master)
2016/06/17 Optimization of a Lab-On-Chip Device to Study the Biocementation of Soils
(Thesis) Main arguer
João Pedro Pereira Valentim (Master)

Association member

Society Organization name Role
2014/01/01 - Current Marie Curie Alumni Association (MCFA) Member Member

Conference scientific committee

Conference name Conference host
2025 - Current I Simpósio Nacional de Mulheres na Nanociência Instituto de Ciências Exatas - ICE - UFJF - Juiz de Fora - Minas Gerais - Brasil
2024 - 2025 2nd and 3rd Portuguese Network on Extracellular Vesicles (PNEV)

Other jury / evaluation

Activity description Institution / Organization
2018/10/04 - Current Thesis Plan, Doctoral Program in Biomedical Engineering, Development of a phage-based lab-on-chip for the multiplex detection of bloodstream pathogens Universidade do Minho Centro de Engenharia Biológica, Portugal