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Ana Batista is an assistant researcher at the Center for Innovative Biomedicine and Biotechnology (CIBB), Faculty of Medicine, University of Coimbra with over 10 years experience in the exciting and growing field of biophotonics. She completed her MSc in Biomedical Engineering in 2010 at the University of Coimbra, Portugal and her PhD in Systems Engineering in 2019 at the Department of Biophotonics and Laser technology, University of Saarland, Germany. Her major research interests focus on the application of biophotonic techniques for medical and biological purposes, leveraging laser technology to advance cell and tissue imaging. Ana has consistently worked on the development and implementation of non-invasive and label-free imaging modalities to advance medical diagnosis. Recently, Ana has expanded her scope to broader biomedical applications, namely, lipid nanoparticle delivery of therapeutic agents in oncology immunotherapy. Throughout her career, Ana has contributed to several research projects, culminating in 21 journal papers, 9 of which as first author and 1 as last author (in press). Evidence of her active engagement with the scientific community is also her contribution to 2 book chapters, over 30 conference proceedings, and over 70 participations in internationally renowned conferences. Ana has also contributed to knowledge dissemination through mentoring of several young researchers, co-organization of several international meetings, and serving as a peer reviewer for several esteemed international journals.
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Identification

Personal identification

Full name
Ana Batista

Citation names

  • Batista, Ana

Author identifiers

Ciência ID
8712-B216-44B6
ORCID iD
0000-0002-5672-8266
Researcher Id
GQZ-8972-2022
Scopus Author Id
7005986533

Knowledge fields

  • Engineering and Technology - Medical Engineering
  • Medical and Health Sciences - Medical Biotechnology
  • Engineering and Technology - Nano-technology
  • Medical and Health Sciences - Clinical Medicine - Ophthalmology
  • Natural sciences - Biological Sciences - Cell Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
German Elementary (A2) Elementary (A2) Elementary (A2) Elementary (A2)
Portuguese (Mother tongue)
Education
Degree Classification
2022/09/20 - 2022/09/21
Concluded
 Super-Resolution Microscopy Workshop (Outros)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2016/04/01 - 2019/04/08
Concluded
 Systems Engineering (Doktor (PhD))
Universität des Saarlandes, Germany
"Two-photon imaging of the cornea using femtosecond laser microscopes and tomographs" (THESIS/DISSERTATION)
 magna cum laude
2017/09
Concluded
 Medical device advisor under MPG § 31 (Outros)
JenLab GmbH, Germany
2017/09
Concluded
 System Quality Management under EN ISO 13485:2016 (Outros)
JenLab GmbH, Germany
2016/04 - 2016/07
Concluded
 German Course - Level A2/B1 (Outros)
Universität des Saarlandes, Germany
2016/04 - 2016/07
Concluded
 Modern Laser-Microscopy in Cell Biology (Outros)
Universität des Saarlandes, Germany
2013/04 - 2013/08
Concluded
 Laser Safety Officer (Outros)
Faculty of Physics and Mechatronics, Saarland University, Germany
2013 - 2013
Concluded
 Laboratory Animal Sciences - Category B (Outros)
Universidade do Porto, Portugal
2013 - 2013
Concluded
 German - Level A1 (Curso médio)
Universität des Saarlandes, Germany
2011
Concluded
 BrainVoyager QX Basic Training Course (Curso médio)
Institute of Biomedical Research in Light and Image, Portugal
2005/10 - 2010/09
Concluded
 Integrated Master's in Biomedical Engineering (Mestrado)
Major in Neurosciences
Universidade de Coimbra, Portugal
"Exploring the Impact of ecstasy on retinal physiology: a pioneer study" (THESIS/DISSERTATION)
Affiliation

Science

Category
Host institution 		Employer
2023/05 - Current Auxiliary Researcher (Research) Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
2020/05/01 - 2023/04 Postdoc (Research) Coimbra Institute for Biomedical Imaging and Translational Research, Portugal
2019/01/01 - 2020/04/30 Postdoc (Research) Department of Biophotonics and Laser Technology, Saarland University, Germany
2013/03/01 - 2018/12/31 Researcher (Research) Department of Biophotonics and Laser Technology, Saarland University, Germany
2016/06/01 - 2017/11/30 Researcher (Research) JenLab GmbH, Germany
2011/05/01 - 2015/05/31 Researcher (Research) Institute of Biomedical Research in Light and Image, Portugal
2009/07/01 - 2010/09/15 Research Trainee (Research) Institute of Biomedical Research in Light and Image, Portugal
Projects

Grant

Designation Funders
2020/05 - 2023/10/31 Optical Coherence Elastography for imaging retina mechanical properties
PTDC/EMD-EMD/32162/2017
Post-doc
Coimbra Institute for Biomedical Imaging and Translational Research, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2017/12 - 2020/04 Laser assisted transfection and manipulation of cells in a microfluidic setup
Project number 285718390
Researcher
Department of Biophotonics and Laser Technology, Saarland University, Germany
 Deutsche Forschungsgemeinschaft
Ongoing
2016/09 - 2017/11 In vivo histology using femtosecond laser multiphoton tomograph for the early diagnosis of skin cancer and corneal diseases while simultaneously reducing Europe’s health care costs
Grant agreement ID: 726666
PhD Student Fellow
JenLab GmbH, Germany
 Horizon 2020
Concluded
2016/06 - 2017/11 MMG-Multimodale Multiphotonen Gewebeanalyse der Humanen Cornea
16KN053020
PhD Student Fellow
JenLab GmbH, Germany
Concluded
2013/03 - 2016/03 SPP 1327: Optically Generated sub-100-nm Structures for Biomedical and Technical Applications
Project number 42963315
Researcher
Department of Biophotonics and Laser Technology, Saarland University, Germany
 Deutsche Forschungsgemeinschaft
Concluded
2013/01 - 2015/05 Fluorescence Lifetime Microscope for Clinical Metabolic Imaging of Corneal Tissues
PTDC/SAU-ENB/122128/2010
Researcher
Institute of Biomedical Research in Light and Image, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2011/04 - 2013/01 neuroCornea - Diabetic peripheral neuropathy early diagnosis and follow-up through in vivo automatic analysis of corneal nerves morphology
PTDC/SAU-BEB/104183/2008
Researcher
Institute of Biomedical Research in Light and Image, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2023/05/04 - Current CiNTech: Technological Hub for Innovation, Translation and Industrialization of Complex Injectable Drugs
02/C05-i01.01/2022.PC644865576-00000005
Post-doc
European Commission
Ongoing
Outputs

Publications

Book chapter
  1. Batista, Ana; Breunig, Hans Georg; Seitz, Berthold; König, Karsten. "Towards Improving Human Corneal Care Using Two-Photon Imaging". In XV Mediterranean Conference on Medical and Biological Engineering and Computing – MEDICON 2019, 1805-1815. Springer International Publishing, 2019.
    Published • 10.1007/978-3-030-31635-8_219
  2. Batista, Ana; Breunig, Hans Georg; Donitzky, Christoph; König, Karsten. "16 Two-photon microscopy and fluorescence lifetime imaging of the cornea". In Multiphoton Microscopy and Fluorescence Lifetime Imaging, 301-320. De Gruyter, 2018.
    Published • 10.1515/9783110429985-018
Conference abstract
  1. Pedro Guimarães; Maria Mateus; António Miguel Morgado; Ana Batista. "Quantitative analysis of collagen organization from second-harmonic generation images (Conference Presentation)". 2023.
    10.1117/12.2645686
  2. Ana Batista; Pedro Serranho; Mário Santos; Carlos Correia; José Domingues; Custódio Loureiro; João Cardoso; et al. "Novel method for higher-resolution displacement computation in phase-resolved optical coherence elastography". Paper presented in Focus on Microscopy (FOM) 2022, Porto, 2022.
    Published
  3. Frueh, Beatrice E.; Batista, Ana; König, Karsten; Seiler, Theo G.. "Riboflavin Concentrations at the Endothelium during Corneal Crosslinking (CXL)". Paper presented in ARVO Annual Meeting 2019, Vancouver, 2019.
    Published
  4. Breunig, Hans Georg; Batista, Ana; König, Aisada; König, Karsten. "Microfluidic setup for laser-assisted cell transfection". Paper presented in Focus on Microscopy (FOM) 2019, London, 2019.
  5. Batista, Ana; Breunig, Hans Georg; Flockerzi, Elias; Seitz, Berthold; König, Karsten. "Femtosecond laser multiphoton tomography for corneal collagen crosslinking evaluation". Paper presented in International CXL Experts' Meeting, Zurich, 2018.
  6. Batista, Ana; Breunig, Hans Georg; König, Aisada; König, Karsten. "In vivo breast skin imaging using multiphoton tomography". Paper presented in ISBS - World Congress on Biophysics and Imaging of the Skin, San Diego, 2018.
  7. Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold. "Two-photon cornea imaging". Paper presented in 12th Workshop and Conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2018), Berlin, 2018.
  8. Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold; König, Karsten. "FLIM of cross-linked corneas". Paper presented in 11th Workshop and Conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2017), Berlin, 2017.
  9. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; König, Karsten. "In vivo imaging of the human eyelid skin by multiphoton tomography". Paper presented in Focus on Microscopy (FOM) 2017, Bordeaux, 2017.
  10. König, Karsten; Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold. "Non-invasive, Label-free, High-resolution optical histology of the human cornea using multiphoton tomography (MPT)". Paper presented in Deutsche Ophthalmologische Gesellschaft (DOG) Kongress 2017, Berlin, 2017.
  11. Batista, Ana; Breunig, Hans Georg; Wu, Ming-Feng; Hager, Tobias; Seitz, Berthold; Morgado, António Miguel; König, Karsten. "Collagen cross-linked corneas assessment using multiphoton tomography". Paper presented in International CXL Experts' Meeting, Zurich, 2016.
  12. Batista, Ana; Breunig, Hans Georg; Wu, Ming-Feng; Hager, Tobias; Seitz, Berthold; Morgado, António Miguel; König, Karsten. "Multiphoton Microscopy Imaging of ex vivo Collagen Cross-linking Human Corneas". Paper presented in Deutsche Ophthalmologische Gesellschaft (DOG) Kongress 2016, 2016.
  13. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Seitz, Berthold; Morgado, António Miguel; König, Karsten. "Multiphoton microscopy for the diagnosis of corneal pathologies". Paper presented in Focus on Microscopy (FOM) 2016, Taipei, 2016.
  14. Uchugonova, Aisada; Breunig, Hans Georg; Batista, Ana; König, Karsten. "Optical Reprogramming with Ultrashort Femtosecond Laser Pulses". Paper presented in Focus on Microscopy (FOM) 2016, Taipei, 2016.
  15. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Seitz, Berthold; Morgado, António Miguel. "Multiphoton microscopy for corneal pathologies diagnosis". Paper presented in German Physical Society (DPG) - Spring Meeting, Hannover, 2016.
  16. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Morgado, António Miguel; König, Karsten. "Multiphoton Imaging of the Cornea: Functional and Structural Information". Paper presented in VII Annual Meeting of IBILI, Coimbra, 2015.
  17. Batista, Ana; Loureiro, Custódio; Domingues, José Paulo; Silva, José Silvestre; Morgado, António Miguel. "Corneal Metabolic State Assessment by Fluorescence Lifetime Imaging Microscopy". Paper presented in Portuguese Society for Microscopy (SPMicros), Lisboa, 2013.
    Published • 10.1017/s1431927613000652
  18. Batista, Ana; Loureiro, Custódio; Domingues, José Paulo; Silva, José Silvestre; Morgado, António Miguel. "Corneal Metabolic Imaging". Paper presented in 4th Annual Meeting of IBILI, Coimbra, 2012.
  19. Batista, Ana; Loureiro, Custódio; Domingues, José Paulo; Silva, José Silvestre; Morgado, António Miguel. "Corneal respiratory function by FAD autofluorescence lifetime". Paper presented in European Association for Vision and Eye Research (EVER) 2012, Nice, 2012.
    Published • 10.1111/j.1755-3768.2012.4647.x
Conference paper
  1. Rita Bernardo; José Paulo Domingues; Rui Bernardes; António Miguel Morgado; Ana Batista. "Evaluation of skin care products using optical coherence elastography". 2023.
    10.1117/12.2648348
  2. Correia, Carlos; Batista, Ana; Barbeiro, Sílvia; Cardoso, João; Domingues, José P.; Henriques, Rafael; Loureiro, Custódio; et al. "Time-dependent elastic numerical model for Optical Coherence Elastography of the murine retina". 2023.
    10.1109/enbeng58165.2023.10175350
  3. Batista, Ana; Correia, Carlos; Barbeiro, Sílvia; Cardoso, João; Domingues, José; Henriques, Rafael; Loureiro, Custódio; et al. "Swept-source Phase-Stabilized Optical Coherence Tomography Setup for Elastography". Paper presented in 2nd International Conference on Image Processing and Vision Engineering, Prague, 2022.
    Published • 10.5220/0011126500003209
  4. Serranho, Pedro; Barbeiro, Sílvia; Henriques, Rafael; Batista, Ana; Santos, Mário; Correia, Carlos; Domingues, José; et al. "On the Numerical Solution of the Inverse Elastography Problem for Time-harmonic Excitation". Paper presented in 2nd International Conference on Image Processing and Vision Engineering, Prague, 2022.
    Published • 10.5220/0011125900003209
  5. König, Karsten; Batista, Ana; Zieger, Michael; Kaatz, Martin; Hänßle, Holger; Fink, Christine; Breunig, Hans Georg. "Clinical multimodal multiphoton tomography of pigmented skin lesions with an ultracompact femtosecond fiber laser". Paper presented in SPIE Photonics West 2020, San Francisco, 2020.
    Published • 10.1117/12.2542322
  6. Batista, Ana; Breunig, Hans Georg; König, Karsten. "High-speed imaging of gas-bubble formation during femtosecond-laser cell optoporation". Paper presented in SPIE Photonics West 2020, San Francisco, 2020.
    Published • https://doi.org/10.1117/12.2542313
  7. König, Karsten; Batista, Ana; König, Aisada; Breunig, Hans Georg. "Multimodal multiphoton tomograph using a compact femtosecond fiber laser". Paper presented in SPIE Photonics West 2019, San Francisco, 2019.
    Published • 10.1117/12.2509551
  8. Breunig, Hans Georg; Batista, Ana; König, Aisada; König, Karsten. "Towards laser-assisted microfluidic-cell transfection". Paper presented in SPIE Photonics West 2019, San Francisco, 2019.
    Published • https://doi.org/10.1117/12.2509442
  9. Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold; König, Karsten. "Non-invasive and label-free follow-up of accelerated-crosslinking using multiphoton tomography". Paper presented in SPIE Photonics West 2019, San Francisco, 2019.
    Published • https://doi.org/10.1117/12.2509359
  10. Breunig, Hans G.; Sauer, Benjamin; Batista, Ana. "Rapid vertical tissue imaging with clinical multiphoton tomography". Paper presented in SPIE Photonics Europe 2018, Strasbourg, 2018.
    Published • https://doi.org/10.1117/12.2323441
  11. Breunig, Hans G.; Batista, Ana; Sauer, Benjamin; König, Aisada; König, Karsten. "Femtosecond-laser setups for cell-membrane poration". Paper presented in SPIE Photonics West 2018, San Francisco, 2018.
    Published • https://doi.org/10.1117/12.2287384
  12. Batista, Ana; Breunig, Hans Georg; König, Karsten. "Rapid in vivo vertical tissue sectioning by multiphoton tomography". Paper presented in SPIE Photonics West 2018, San Francisco, 2018.
    Published • 10.1117/12.2286915
  13. König, Karsten; Uchugonova, Aisada; Breunig, Hans Georg; Batista, Ana. "Two-photon FLIM imaging and femtosecond laser nano processing of IPS cells and stem cells (Conference Presentation)". Paper presented in SPIE Photonics West 2018, San Francisco, 2018.
    10.1117/12.2287125
  14. König, Karsten; Batista, Ana; Hager, Tobias; Seitz, Berthold. "Multiphoton tomography of the human eye". Paper presented in SPIE Photonics West 2017, San Francisco, 2017.
    Published • 10.1117/12.2255151
  15. Batista, Ana; Uchugonova, Aisada; Breunig, Hans Georg; König, Karsten. "Towards in vivo breast skin characterization using multiphoton microscopy". Paper presented in SPIE Photonics West 2017, San Francisco, 2017.
    Published • 10.1117/12.2252387
  16. Breunig, Hans Georg; Uchugonova, Aisada; Batista, Ana; König, Karsten. "Femtosecond-laser assisted cell reprogramming". Paper presented in SPIE Photonics West 2017, San Francisco, 2017.
    Published • 10.1117/12.2252088
  17. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; König, Karsten. "In vivo multiphoton imaging of the eyelid skin". Paper presented in SPIE Photonics West 2017, San Francisco, 2017.
    Published • 10.1117/12.2251949
  18. Silva, Susana F.; Batista, Ana; Domingues, José Paulo; Quadrado, Maria João; Morgado, António Miguel. "Time-gated FLIM microscope for corneal metabolic imaging". Paper presented in SPIE Photonics West 2016, San Francisco, 2016.
    Published • 10.1117/12.2214182
  19. Silva, Susana F.; Batista, Ana; Castejón, Olga Ciutad; Quadrado, Maria João; Domingues, José Paulo; Morgado, Miguel. "Development of a time-gated fluorescence lifetime microscope for in vivo corneal metabolic imaging". Paper presented in European Conferences on Biomedical Optics, Munich, 2015.
    Published • 10.1364/ecbo.2015.953709
  20. Silva, Susana Figueiredo; Batista, Ana; Domingues, José Paulo; Quadrado, Maria Joao; Morgado, António Miguel. "Fluorescence lifetime microscope for corneal metabolic imaging". Paper presented in 2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG), Porto, 2015.
    Published • 10.1109/ENBENG.2015.7088878
  21. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Morgado, António Miguel; König, Karsten. "Characterization of porcine eyes based on autofluorescence lifetime imaging". Paper presented in SPIE Photonics West 2015, San Francisco, 2015.
    Published • 10.1117/12.2077563
  22. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Seitz, Berthold; Morgado, António Miguel; König, Karsten. "Two-photon autofluorescence lifetime and SHG imaging of healthy and diseased human corneas". Paper presented in SPIE Photonics West 2015, San Francisco, 2015.
    Published • 10.1117/12.2077569
  23. Uchugonova, Aisada; Breunig, Hans G.; Batista, Ana; König, Karsten. "Optical reprogramming with ultrashort femtosecond laser pulses". Paper presented in SPIE Photonics West 2015, San Francisco, 2015.
    Published • 10.1117/12.2080872
  24. Breunig, Hans G.; Batista, Ana; Uchugonova, Aisada; König, Karsten. "Motionless polarization-resolved second harmonic generation imaging of corneal collagen". Paper presented in SPIE Photonics West 2015, San Francisco, 2015.
    Published • 10.1117/12.2078701
  25. Breunig, Hans Georg; Batista, Ana; Uchugonova, Aisada; König, Karsten. "Software aided automatic cell optoporation system". Paper presented in SPIE Photonics West 2015, San Francisco, 2015.
    Published • 10.1117/12.2078728
  26. Uchugonova, Aisada; Breunig, Hans Georg; Batista, Ana; König, Karsten. "Optical cell cleaning with NIR femtosecond laser pulses". Paper presented in SPIE Photonics West 2015, San Francisco, 2015.
    Published • 10.1117/12.2080867
  27. Batista, Ana; Morgado, António Miguel; König, Karsten. "Detection of back-reflected SHG from corneal histological sections". Paper presented in SPIE Photonics West 2014, San Francisco, California, 2014.
    Published • 10.1117/12.2038251
  28. Batista, Ana; Breunig, Georg; Uchugonova, Aisada; Berthold Seitz; Morgado, António Miguel; König, Karsten. "Label-free SHG imaging and spectral FLIM of corneas using a sub-15 fs laser microscope". Paper presented in SPIE Photonics West 2014, San Francisco, California, 2014.
    Published • 10.1117/12.2037640
  29. Uchugonova, Aisada; Batista, Ana; König, Karsten. "Fluorescence lifetime imaging of induced pluripotent stem cells". Paper presented in SPIE Photonics West 2014, San Francisco, California, 2014.
    Published • 10.1117/12.2037662
  30. Batista, Ana; Silva, Susana Figueiredo; Domingues, José Paulo; Morgado, António Miguel. "Corneal metabolic imaging by FAD autofluorescence lifetime". Paper presented in 2013 Portuguese BioEngineering Meeting IEEE-EMBS, Braga, Portugal, 2013.
    Published • 10.1109/enbeng.2013.6518396
  31. Batista, Ana; Loureiro, Custódio; Domingues, José Paulo; Silva, José Silvestre; Morgado, António Miguel. "FLIM as a Tool for Metabolic Imaging of the Cornea". Paper presented in 2nd Portuguese BioEngineering Meeting IEEE-EMBS, Coimbra, 2012.
    Published • http://dx.doi.org/10.1109/enbeng.2012.6331350
  32. Batista, Ana. "Exploring the impact of ecstasy on retinal physiology: A pioneer study". Paper presented in 2nd Portuguese BioEngineering Meeting IEEE-EMBS, Coimbra, 2012.
    Published • 10.1109/enbeng.2012.6331389
  33. Batista, Ana; Loureiro, Custódio; Domingues, José Paulo; Silva, José Silvestre; Morgado, António Miguel. "Corneal Cells Metabolic Imaging using FAD Fluorescence Lifetime". Paper presented in Latin America Optics and Photonics Conference, São Sebastião, Brazil, 2012.
    Published • 10.1364/laop.2012.lt1a.5
Conference poster
  1. Rita Bernardo; Domingues, José Paulo; Rui C Bernardes; António Miguel Morgado; Batista, Ana. "Evaluating the efficacy of skin care products using optical coherence elastography". Paper presented in 2nd Annual Meeting of CIBIT, 2023.
  2. Guimarães, Pedro; António Miguel Morgado; Batista, Ana. "Quantifying Collagen Organization using Second Harmonic Generation Imaging". Paper presented in INL Global - Local Photonics Meetup, 2023.
  3. Batista, Ana; José Paulo Domingues; Correia, Carlos; Barbeiro, Sílvia Alexandra Alves ; Cardoso, Joao; Jordão, João; Henriques, Rafael; et al. "Phase-resolved optical coherence elastography methods and instrumentation". Paper presented in 1st Annual meeting of CIBIT, 2022.
  4. Ana Batista; Guimarães, Pedro; Michael Zieger; Martin Kaatz; Karsten König. "Multiphoton tomography combined with artificial intelligence for automatic diagnosis of skin conditions". Paper presented in ISBS - World Congress on Biophysics and Imaging of the Skin, 2021.
  5. Batista, Ana; Breunig, Hans Georg; Flockerzi, Elias; Seitz, Berthold; König, Karsten. "Corneal collagen crosslinking and treatment outcome evaluation based on femtosecond lasers". Paper presented in SPIE Photonics West 2020, 2020.
  6. Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold; König, Karsten. "Non-invasive and label-free follow-up of accelerated-crosslinking using multiphoton tomography". Paper presented in SPIE Photonics West 2019, 2019.
  7. Batista, Ana; Breunig, Hans Georg; Flockerzi, Elias; Seitz, Berthold; König, Karsten. "Multiphoton tomography for accelerated- corneal collagen crosslinking follow-up". Paper presented in International CXL Experts' Meeting, 2018.
  8. Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold; König, Karsten. "Multiphoton tomography of ex vivo cross-linked human corneas". Paper presented in SPIE Photonics West 2018, 2018.
  9. Batista, Ana; Uchugonova, Aisada; Breunig, Hans Georg; König, Karsten. "Towards in vivo characterization of breast skin using multiphoton tomography". Paper presented in 11th Workshop and Conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2017), 2017.
  10. Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold; Morgado, António Miguel; König, Karsten. "Ex vivo evaluation of collagen crosslinking corneas using multiphoton tomography". Paper presented in 16th International Photodynamic Association World Congress, 2017.
  11. Uchugonova, Aisada; Breunig, Hans Georg; Batista, Ana; König, Karsten. "Optical transfection with a microfluidic cell system using sub-15 femtosecond lasers". Paper presented in Focus on Microscopy (FOM) 2017, 2017.
  12. Batista, Ana; Uchugonova, Aisada; Breunig, Hans Georg; König, Karsten. "Towards in vivo breast skin characterization using multiphoton microscopy". Paper presented in SPIE Photonics West 2017, 2017.
  13. Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold; König, Karsten. "Evaluation of collagen crosslinked corneas using multiphoton tomography". Paper presented in Deutsche Ophthalmologische Gesellschaft (DOG) Kongress 2017, 2017.
  14. Batista, Ana; Breunig, Hans Georg; Wu, Ming-Feng; Hager, Tobias; Seitz, Berthold; Morgado, António Miguel; König, Karsten. "Multiphoton tomography: a new imaging modality for corneal evaluation". Paper presented in International CXL Experts' Meeting, 2016.
  15. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Morgado, António Miguel; König, Karsten. "Two-Photon Microscopy of Skin and Eye". Paper presented in ISBS - World Congress on Biophysics and Imaging of the Skin, 2016.
  16. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Seitz, Berthold; Morgado, António Miguel; König, Karsten. "Two-photon autofluorescence lifetime and SHG imaging of healthy and diseased human corneas". Paper presented in SPIE Photonics West 2015, 2015.
  17. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Morgado, António Miguel; König, Karsten. "Two-Photon Autofluorescence Lifetime and SHG Imaging of Healthy and Diseased Human Corneas". Paper presented in VI Annual Meeting of IBILI, 2014.
  18. Batista, Ana; Morgado, António Miguel; König, Karsten. "Detection of back-reflected SHG from corneal histological sections". Paper presented in SPIE Photonics West 2014, 2014.
  19. Batista, Ana; Morgado, António Miguel; König, Karsten. "Porcine eye imaging with a sub-15 fs laser microscope". Paper presented in 9th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2014), 2014.
  20. Batista, Ana; Berthold Seitz; Uchugonova, Aisada; Breunig, Hans Georg; Morgado, Miguel; König, Karsten. "Label-free Corneal Imaging with a sub-15fs Laser Microscope". Paper presented in V Annual Meeting of IBILI, 2013.
  21. Batista, Ana; Uchugonova, Aisada; Breunig, Georg; Morgado, Miguel; König, Karsten. "Corneal SHG and Autofluorescence with sub-15 fs Laser Microscopy". Paper presented in 8th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2013), 2013.
  22. Batista, Ana; Loureiro, Custódio; Domingues, José Paulo; Silva, José Silvestre; Morgado, António Miguel. "Metabolic Imaging of the Cornea using FLIM: Preliminary Results". Paper presented in 7th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2012), 2012.
  23. Batista, Ana; Loureiro, Custódio; Domingues, José Paulo; Silva, José Silvestre; Morgado, António Miguel. "FLIM as a Tool for Metabolic Imaging of the Cornea". Paper presented in 2nd Portuguese BioEngineering Meeting IEEE-EMBS, 2012.
Journal article
  1. Guimarães, Pedro; António Miguel Morgado; Batista, Ana. "On the Quantitative Analysis of Lamellar Collagen Arrangement with Second-Harmonic Generation Imaging". Biomedical Optics Express (2024):
    Open access • In press
  2. Ana Batista; Pedro Serranho; Mário J. Santos; Carlos Correia; José P. Domingues; Custódio Loureiro; João Cardoso; et al. "Phase-Resolved Optical Coherence Elastography: An Insight into Tissue Displacement Estimation". Sensors (2023): https://doi.org/10.3390/s23083974.
    10.3390/s23083974
  3. Ana Batista; Pedro Guimarães; Pedro Serranho; Ana Nunes; João Martins; Paula I. Moreira; António Francisco Ambrósio; et al. "Retinal imaging in animal models: Searching for biomarkers of neurodegeneration". Frontiers in Ophthalmology 3 (2023): http://dx.doi.org/10.3389/fopht.2023.1156605.
    10.3389/fopht.2023.1156605
  4. Ana Batista; Pedro Guimarães; João Martins; Paula I. Moreira; António Francisco Ambrósio; Miguel Castelo-Branco; Pedro Serranho; Rui Bernardes. "Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's disease". Frontiers in Aging Neuroscience 15 (2023): http://dx.doi.org/10.3389/fnagi.2023.1161847.
    10.3389/fnagi.2023.1161847
  5. Ana Batista; Pedro Guimarães; José Paulo Domingues; Maria João Quadrado; António Miguel Morgado. "Two-Photon Imaging for Non-Invasive Corneal Examination". Sensors (2022): https://doi.org/10.3390/s22249699.
    10.3390/s22249699
  6. Breunig, Hans Georg; Batista, Ana; König, Karsten. Corresponding author: Breunig, Hans Georg. "Vertical Multiphoton Imaging of the Human Skin in vivo". Journal of Clinical and Medical Images 6 17 (2022): 1-7.
    Published
  7. Guimarães, Pedro; Batista, Ana; Zieger, Michael; Kaatz, Martin; König, Karsten. "Artificial Intelligence in Multiphoton Tomography: Atopic Dermatitis Diagnosis". Scientific Reports 10 1 (2020): 1-9.
    Open access • Published • 10.1038/s41598-020-64937-x
  8. König, Karsten; Breunig, Hans Georg; Batista, Ana; Schindele, Andreas; Zieger, Michael; Kaatz, Martin. "Translation of two-photon microscopy to the clinic: multimodal multiphoton CARS tomography of in vivo human skin". Journal of Biomedical Optics 25 1 (2020): 014515-1-014515-12.
    Open access • Published • https://doi.org/10.1117/1.JBO.25.1.014515
  9. Batista, Ana; Breunig, Hans Georg; Hager, Tobias; Seitz, Berthold; König, Karsten. "Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging". Scientific Reports 9 1 (2019): 1-9. http://dx.doi.org/10.1038/s41598-019-46572-3.
    Open access • Published • 10.1038/s41598-019-46572-3
  10. Seiler, Theo G.; Batista, Ana; Frueh, Beatrice E.; König, Karsten. "Riboflavin Concentrations at the Endothelium During Corneal Cross-Linking in Humans". Investigative Opthalmology & Visual Science 60 6 (2019): 2140-2145. http://dx.doi.org/10.1167/iovs.19-26686.
    Open access • Published • 10.1167/iovs.19-26686
  11. Batista, Ana; Breunig, Hans G.; König, Aisada; Morgado, António Miguel; König, Karsten. "Assessment of the metabolism and morphology of the porcine cornea, lens and retina by 2-photon imaging". Journal of Biophotonics 11 7 (2018): e201700324. http://dx.doi.org/10.1002/jbio.201700324.
    Published • 10.1002/jbio.201700324
  12. Batista, Ana; Breunig, Hans Georg; König, Aisada; Schindele, Andreas; Hager, Tobias; Seitz, Berthold; König, Karsten. "High-resolution, label-free two-photon imaging of diseased human corneas". Journal of Biomedical Optics 23 03 (2018): 036002-1-036002-8. http://dx.doi.org/10.1117/1.jbo.23.3.036002.
    Open access • Published • 10.1117/1.jbo.23.3.036002
  13. Batista, Ana; Breunig, Hans Georg; König, Aisada; Schindele, Andreas; Hager, Tobias; Seitz, Berthold; Morgado, António Miguel; König, Karsten. "Assessment of Human Corneas Prior to Transplantation Using High-Resolution Two-Photon Imaging". Investigative Opthalmology & Visual Science 59 1 (2018): 176-184. http://dx.doi.org/10.1167/iovs.17-22002.
    Open access • Published • 10.1167/iovs.17-22002
  14. Breunig, Hans Georg; Batista, Ana; Uchugonova, Aisada; König, Karsten. "Cell optoporation with a sub-15 fs and a 250-fs laser". Journal of Biomedical Optics 21 6 (2016): 060501. http://dx.doi.org/10.1117/1.jbo.21.6.060501.
    Open access • Published • 10.1117/1.jbo.21.6.060501
  15. Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Morgado, António Miguel; König, Karsten. "Two-photon spectral fluorescence lifetime and second-harmonic generation imaging of the porcine cornea with a 12-femtosecond laser microscope". Journal of Biomedical Optics 21 3 (2016): 036002. http://dx.doi.org/10.1117/1.jbo.21.3.036002.
    Open access • Published • 10.1117/1.jbo.21.3.036002
  16. Uchugonova, Aisada; Breunig, Hans Georg; Batista, Ana; König, Karsten. "Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses". Journal of Biomedical Optics 20 11 (2015): 115008. http://dx.doi.org/10.1117/1.jbo.20.11.115008.
    Open access • 10.1117/1.jbo.20.11.115008
  17. Uchugonova, Aisada; Breunig, Hans Georg; Batista, Ana; König, Karsten. "Optical reprogramming of human cells in an ultrashort femtosecond laser microfluidic transfection platform". Journal of Biophotonics 9 9 (2015): 942-947. http://dx.doi.org/10.1002/jbio.201500240.
    Published • 10.1002/jbio.201500240
  18. Georg Breunig, Hans; Uchugonova, Aisada; Batista, Ana; König, Karsten. "Software-aided automatic laser optoporation and transfection of cells". Scientific Reports 5 1 (2015): http://dx.doi.org/10.1038/srep11185.
    Open access • Published • 10.1038/srep11185
  19. Breunig, Hans Georg; Uchugonova, Aisada; Batista, Ana; König, Karsten. "High-throughput continuous flow femtosecond laser-assisted cell optoporation and transfection". Microscopy Research and Technique 77 12 (2014): 974-979. http://dx.doi.org/10.1002/jemt.22423.
    Published • 10.1002/jemt.22423
  20. Batista, Ana; Silva, Susana Figueiredo; Domingues, José Paulo; Morgado, António Miguel. "Fluorescence Lifetime Based Corneal Metabolic Imaging". Procedia Technology 17 (2014): 281-288. http://dx.doi.org/10.1016/j.protcy.2014.10.238.
    Open access • Published • 10.1016/j.protcy.2014.10.238
  21. Martins, João; Castelo-Branco, Miguel; Batista, Ana; Oliveiros, Bárbara; Santiago, Ana Raquel; Galvão, Joana; Fernandes, Eduarda; et al. "Effects of 3,4-Methylenedioxymethamphetamine Administration on Retinal Physiology in the Rat". PLoS ONE 6 12 (2011): 29583-29583.
    Open access • Published • 10.1371/journal.pone.0029583
Journal issue
  1. Batista, Ana; Karsten König. "Non-invasive Skin Imaging Development and Applications". Special issue of Life (2023): https://www.mdpi.com/journal/life/special_issues/950FL71550#editors.
    Invited editor
Newspaper article
  1. König, Karsten; Batista, Ana; Breunig, Hans Georg; Berthold Seitz. "Multiphotonen-Tomographie der humanen Kornea", Ophthalmologische Nachrichten Special Bilddiagnostik, 2014
Thesis / Dissertation
  1. Batista, Ana. "Two-photon imaging of the cornea using femtosecond laser microscopes and tomographs". PhD, Universität des Saarlandes, 2018. https://publikationen.sulb.uni-saarland.de/handle/20.500.11880/27410.
  2. Batista, Ana. "Exploring the impact of ecstasy on retinal physiology: A pioneer study". Master, 2010. https://estudogeral.sib.uc.pt/bitstream/10316/14170/1/Tese_MIEB_Ana%20Batista.pdf.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/02 Evaluation of skin care products using optical coherence elastography SPIE Photonics West 2023
(San Francisco, United States)
2023/02 Quantitative analysis of collagen organization from second-harmonic generation images SPIE Photonics West 2023
(San Francisco, United States)
2022/04 Swept-source Phase-Stabilized Optical Coherence Tomography Setup for Elastography Improve – Imaging4OND, 2022
2020/02 High-speed imaging of gas-bubble formation during femtosecond-laser cell optoporation SPIE Photonics West 2020
(San Francisco, United States)
2019/09 Towards Improving Human Corneal Care Using Two-Photon Imaging XV Mediterranean Conference on Medical and Biological Engineering and Computing – MEDICON 2019
(Coimbra, Portugal)
2019/02 High-resolution, non-invasive breast skin imaging in vivo using multiphoton tomography SPIE Photonics West 2019
SPIE (San Francisco, United States)
2018/06 Two-photon cornea imaging 12th Workshop and Conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2018)
(Berlin, Germany)
2018/02 Rapid in vivo vertical tissue sectioning by multiphoton tomography SPIE Photonics West 2018
(San Francisco, United States)
2018/02 Femtosecond-laser setups for cell-membrane poration SPIE Photonics West 2018
(San Francisco, California, United States)
2018 In vivo breast skin imaging using multiphoton tomography ISBS - World Congress on Biophysics and Imaging of the Skin
(San Diego, United States)
2018 Femtosecond laser multiphoton tomography for corneal collagen crosslinking evaluation International CXL Experts' Meeting
(Zurich, Switzerland)
2017/06 FLIM of cross-linked corneas 11th Workshop and Conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2017)
(Berlin, Germany)
2017/02 In vivo multiphoton imaging of the eyelid skin SPIE Photonics West 2017
(San Francisco, California, United States)
2017 In vivo imaging of the human eyelid skin by multiphoton tomography Focus on Microscopy (FOM) 2017
(Bordeaux, France)
2015/02 Characterization of porcine eyes based on autofluorescence lifetime imaging SPIE Photonics West 2015
(San Francisco, California, United States)
2014/02 Label-free SHG imaging and spectral FLIM of corneas using a sub-15 fs laser microscope SPIE Photonics West 2014
(San Francisco, California, United States)
2013 Corneal Metabolic Imaging by FAD autofluorescence lifetime 3rd Portuguese Bioengineering Meeting IEEE-EMBS
IEEE-EMBS (Braga, Portugal)
2012/12 Corneal Metabolic Imaging 4th Annual Meeting of IBILI
IBILI (Coimbra, Portugal)
2012/11 Imagiologia Metabólica da Córnea por Técnicas de Tempo de Vida de Fluorescência: Análise de Segurança Laser III Congresso de Proteção Contra Radiações dos Países e Comunidades de Língua Portuguesa
Sociedade Brasileira de Proteção Radiológica & Sociedade Portuguesa de Proteção contra Radiações (Lisboa, Portugal)
2012/09 Corneal Metabolic State Assessment by Fluorescence Lifetime Imaging Microscopy XLVI Congress of the Portuguese Society for Microscopy 2012
Sociedade Portuguesa de Microscopia (Lisboa, Portugal)
2012/02 Exploring the impact of ecstasy on retinal physiology: A pioneer study 2nd Portuguese BioEngineering Meeting IEEE-EMBS
IEEE (Coimbra, Portugal)
2012 Corneal respiratory function by FAD autofluorescence lifetime European Association for Vision and Eye Research (EVER) 2012
(Nice, France)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021/09 - 2024/02/29 Development of phase-sensitive optical coherence elastography with ultrasound impulses for ophthalmological imaging
Co-supervisor of Maria Inês Caldeira
 Master in Physics Engineering (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2023/02 - 2023/07 OCT signal characterization in different animal imaging systems
Co-supervisor
 Bachelor's Degree in Biomedical Engineering (Degree)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2023/02 - 2023/07 Optical Coherences Elastography in Dermatology
Co-supervisor of Ana Carolina Eloy de Castro
 Bachelor's Degree in Biomedical Engineering (Degree)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2023/02 - 2023/07 Optical Coherences Elastography in Dermatology
Co-supervisor of Roksolana Shevchenko
 Bachelor's Degree in Biomedical Engineering (Degree)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2021/09 - 2022/09 Quantitative evaluation of collagen fiber organization from backward-detected SHG images
Co-supervisor of Maria Mateus
 Master in Physics Engineering (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2021/09 - 2022/09 Optical Coherence Elastography (OCE) in dermatology
Co-supervisor of Rita Bernardo
 Master’s Degree in Biomedical Engineering (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2022/02 - 2022/07 Fluorescence lifetime imaging microscopy of stained histological sections
Co-supervisor of Rita Inácio Matos
Bachelor's Degree in Biomedical Engineering (Degree)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2014 - 2020 Bi-anual Hands-on-Workshop on multiphoton imaging microscopy (2014 - 2020)
Workshop (Co-organisor)
 Department of Biophotonics and Laser Technology, Saarland University, Germany
2018/06/15 - 2018/06/16 12th Workshop and conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques FLIM 2018 (2018/06/15 - 2018/06/16)
Conference (Member of the Organising Committee)
 Department of Biophotonics and Laser Technology, Saarland University, Germany
2017/06/30 - 2017/07/01 11th Workshop and conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques FLIM 2017 (2017/06/30 - 2017/07/01)
Conference (Member of the Organising Committee)
 Department of Biophotonics and Laser Technology, Saarland University, Germany
2015/06/17 - 2015/06/19 10th Workshop and conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques FLIM 2015 (2015/06/17 - 2015/06/19)
Conference (Member of the Organising Committee)
 Department of Biophotonics and Laser Technology, Saarland University, Germany
2014/05/27 - 2014/05/28 9th Workshop and conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques FLIM 2014 (2014/05/27 - 2014/05/28)
Conference (Member of the Organising Committee)
 Department of Biophotonics and Laser Technology, Saarland University, Germany
2013/07/01 - 2013/07/02 8th Workshop and conference on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques FLIM 2013 (2013/07/01 - 2013/07/02)
Conference (Member of the Organising Committee)
 Department of Biophotonics and Laser Technology, Saarland University, Germany

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2024/02/29 Development of phase-sensitive optical coherence elastography with ultrasound impulses for ophthalmological imaging
Supervisor
 Maria Inês Caldeira (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2023/09/25 Implementation of a customised light-sheet microscope
(Thesis) Arguer
 Matilde Antunes Palmeira (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2022/09/28 Quantitative evaluation of collagen fiber organization from SHG images
Supervisor
 Maria Alves Mateus (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2022/09/27 Optical Coherence Elastography (OCE) in dermatology
Supervisor
 Rita Bernardo (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal

Association member

Society Organization name Role
2015/01 - 2021/01 SPIE - the international society for optics and photonics Member
2018 - 2020 ARVO - The Association for Research in Vision and Ophthalmology Member
2016/01 - 2017/12 Deutsche Physikalische Gesellschaft (DPG) Member

Committee member

Activity description
Role 		Institution / Organization
2023/11/20 - Current Topical Advisory Panel Member: Life, an Open Access Journal by MDPI
Member

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2017 - 2020 "Lasers in Ophthalmology" and "Lasers in Nanosurgery: Laser Assisted Transfection" lectures within "Laser in Medicine and Nanobiotechnology" Course Module Systems Engineering (Master) Department of Biophotonics and Laser Technology, Saarland University, Germany
2017 - 2019 "Femtosecond-laser transfection and cell reprogramming" and "Non-invasive and label-free imaging of the eye" lectures within "Laser in Material Processing" Course Module Systems Engineering (Master) Department of Biophotonics and Laser Technology, Saarland University, Germany

Journal scientific committee

Journal title (ISSN) Publisher
2023/08 - Current Diagnostics (2075-4418) MDPI
2023/06 - Current Life (2075-1729) MDPI
2023/04 - Current Biomedical Optics Express (2156-7085) Optica
2023/02 - Current Scientific Reports (2045-2322) Nature Portfolio
2020/02 - Current Current Eye Research (0271-3683) Taylor & Francis
2019/07 - Current Investigative Ophthalmology & Visual Science (0146-0404) Association for Research in Vision and Ophthalmology
2018/10 - Current Translational Vision Science & Technology (2164-2591) Association for Research in Vision and Ophthalmology
Distinctions

Award

2022 Sensors 2023 Travel Award
MDPI AG, Switzerland
2016 Best Poster Award
International Society for Biophysics and Imaging of the Skin, Portugal
2014 Best Poster Award
VI Annual Meeting of IBILI, Portugal
2012 Honorable mention to the Master thesis "Exploring the impact of ecstasy on retinal physiology: a pioneer study"
Scientific committee of the 2nd Portuguese BioEngineering Meeting IEEE-EMBS, Portugal

Other distinction

2023 Invited Speaker - Multiphoton Microscopy in the Biomedical Sciences XXIII, SPIE, San Francisco, USA Photonics West 2023
2023 Invited Speaker - 3ª Reunión Anual de Investigación Biomédica 2023, Hermosillo, Mexico (remote)