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I start my research work in 1984 supervised by Prof. Joaquim S. Cabral. Since then my research interests are in Biochemical Engineering area, mainly in protein and plasmid DNA separation and purification, membrane processes and process integration and simulationI am a faculty member of the former Institute for Biotechnology and Bioengineering (IBB) and of the Centre for Biological and Chemical Engineering (CEBQ), at Instituto Superior Técnico. My current research is based on bioprocess design and economic assessment, focused on plasmid DNA (pDNA) manufacturing processes (production, separation, purification, quality control and monitoring) for application in gene therapy or DNA vaccination. The utilization of membrane processes (ultra and microfiltration) and the development of alternative methods for plasmid release are under research. The development of alternative microbial GRAS (generally recognized as safe) platforms for pDNA and lactic acid production and the production/purification of carotenoids/lipids using symbiotic microalgae/yeasts cultures are other goals of my research.
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Identification

Personal identification

Full name
José António Leonardo dos Santos

Citation names

  • Santos, José

Author identifiers

Ciência ID
7C1F-2FEA-8FBC

Telephones

Mobile phone
  • 919191647 (Personal)

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology - Bioprocessing Technologies

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Proficiency (C2) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
1989 - 1996/11
Concluded
 Engenharia Química (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Estudos de Separação de uma Proteína Recombinante por Processos de Membranas " (THESIS/DISSERTATION)
 Distinção e Louvor
1986 - 1988
Concluded
 Biotecnologia (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Recuperação de Proteases Alcalinas por ultra-microfiltração " (THESIS/DISSERTATION)
 Muito Bom
1977/10 - 1985/04
Concluded
 Engenharia Química - Processos e Indústria (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
 14 valores
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
1996/11 - Current Assistant Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
1988/06/01 - 1996/11/19 Assistant (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
1985/04 - 1986/07 Trainee Assistant (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
1984/10 - 1985/04 Tutor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
Projects

Contract

Designation Funders
2012/01/01 - 2014/12/31 DSPMab - Intensificação do processo de purificação de anticorpos monoclonais
PTDC/EQU-EPR/117427/2010
Researcher
Concluded
2011/01/01 - 2013/12/31 DELTRA: Improvement of the immunogenicity of DNA vaccines
PTDC/EBB-EBI/113650/2009
Researcher
2010/02/01 - 2013/01/31 Membrane chromatographic matrices for plasmid-DNA purification: rational design based on biochemical and biophysical interactions
PTDC/EBB-EBI/100925/2008
Researcher
Concluded
2007 - 2010 DNAvac: Síntese, optimização e Intensificação de Processos de Biosseparação para Produção de Vacinas de DNA
PTDC/QUE/65492/2006
Researcher
Concluded
2006 - 2010 BIOPRODUCTION: Sustainable Microbial And Biocatalytic Production of Advanced Functional Materials
funding entity: E.C.
Researcher
2000/10 - 2002/09 PÂNCREAS BIO-ARTIFICIAL: Estudos preliminares de hemocompatibilidade, difusão e isolamento imunológico
FCT/PRAXIS XXI (nº PRAXIS/BIO/12007/1998)
Researcher
Concluded
1997/01 - 2000/09 ECOCOURO
PEDIP II (Projectos Mobilizadores para o Desenvolvimento Tecnológico) (nº 25/00291)
Researcher
Cancelled
1996/12 - 1999/11 Integrated Bioprocess Design for Large Scale Production and Isolation of Recombinant Proteins
BIO4-CT96-0435
Researcher
Concluded
Outputs

Publications

Book chapter
  1. Prazeres, D.M.F.; Santos, J.A.L.. "Production and Purification of Adenovirus Vectors for Gene Therapy". In Handbook of Pharmaceutical Biotechnology, 1261-1295. John Wiley & Sons, Inc., 2007.
    Published • 10.1002/9780470117118.ch10a
  2. Santos, José; Duarte Miguel Prazeres; Sindélia Freitas. "Plasmid DNA production". In Development of sustainable bioprocesses: Modeling and assessment, edited by Arno Biwer; Elmar Heinzle; Charles Cooney, 271-285. John Wiley, 2006.
    Published
  3. Santos, José; Marília Clemente Velez Mateus; Joaquim M.S. Cabral (AB13-9ED3-C821). "Membrane separation processes". In Recovery Processes for Biological Materials, edited by J.F. Kennedy; Joaquim M.S. Cabral, 177-222. Chichester, United Kingdom: John Wiley & Sons, 1993.
    Published
  4. Santos, José; Joaquim M.S. Cabral; Marília Clemente Velez Mateus. "Pressure-driven membrane processes". In Membrane Engineering, edited by C.A. Costa; Joaquim M.S. Cabral (AB13-9ED3-C821), 177-205. Dordrecht, Netherlands: Kluwer Academic Publishers, 1991.
    Published • xx
Journal article
  1. Dias, Carla; Reis, Alberto; Santos, José A.L.; Gouveia, Luisa; Lopes da Silva, Teresa. "Primary brewery wastewater as feedstock for the yeast Rhodosporidium toruloides and the microalga Tetradesmus obliquus mixed cultures with lipid production". Process Biochemistry 113 (2022): 71-86. http://dx.doi.org/10.1016/j.procbio.2021.12.019.
    Open access • 10.1016/j.procbio.2021.12.019
  2. Dias, Carla; Gouveia, Luísa; Santos, José A. L.; Reis, Alberto; da Silva, Teresa Lopes. "Rhodosporidium toruloides and Tetradesmus obliquus Populations Dynamics in Symbiotic Cultures, Developed in Brewery Wastewater, for Lipid Production". Current Microbiology 79 2 (2022): http://dx.doi.org/10.1007/s00284-021-02683-7.
    Open access • 10.1007/s00284-021-02683-7
  3. Izaguirre, Jon Kepa; Barañano, Leire; Castañón, Sonia; Santos, José A. L.; Cesário, M. Teresa; da Fonseca, M. Manuela R.; Alkorta, Itziar; Garbisu, Carlos. "Economic and environmental assessment of bacterial poly(3-hydroxybutyrate) production from the organic fraction of municipal solid waste". Bioresources and Bioprocessing 8 1 (2021): http://dx.doi.org/10.1186/s40643-021-00392-4.
    Open access • 10.1186/s40643-021-00392-4
  4. Dias, Carla; Gouveia, Luísa; Santos, José A. L.; Reis, Alberto; Lopes da Silva, Teresa. "Using flow cytometry to monitor the stress response of yeast and microalgae populations in mixed cultures developed in brewery effluents". Journal of Applied Phycology (2020): http://dx.doi.org/10.1007/s10811-020-02236-8.
    Published • 10.1007/s10811-020-02236-8
  5. Dias, Carla; Reis, Alberto; Santos, José A.L.; Lopes da Silva, Teresa. "Concomitant wastewater treatment with lipid and carotenoid production by the oleaginous yeast Rhodosporidium toruloides grown on brewery effluent enriched with sugarcane molasses and urea". Process Biochemistry 94 (2020): 1-14. http://dx.doi.org/10.1016/j.procbio.2020.03.015.
    Published • 10.1016/j.procbio.2020.03.015
  6. Dias, Carla; Santos, José; Reis, Alberto; Lopes da Silva, Teresa. "Yeast and microalgal symbiotic cultures using low-cost substrates for lipid production". Bioresource Technology Reports 7 (2019): 100261. http://dx.doi.org/10.1016/j.biteb.2019.100261.
    10.1016/j.biteb.2019.100261
  7. Marques, S.; Matos, C.T.; Gírio, F.M.; Roseiro, J.C.; Santos, J.A.L.. "Lactic acid production from recycled paper sludge: Process intensification by running fed-batch into a membrane-recycle bioreactor". Biochemical Engineering Journal 120 (2017): 63-72. http://dx.doi.org/10.1016/j.bej.2016.12.021.
    10.1016/j.bej.2016.12.021
  8. Marques, S.; Matos, C.T.; Gírio, F.M.; Roseiro, J.C.; Santos, J.A.L.. "Lactic acid production from recycled paper sludge: Process intensification by running fed-batch into a membrane-recycle bioreactor". Biochemical Engineering Journal 120 (2017): 63-72. http://dx.doi.org/10.1016/j.bej.2016.12.021.
    Published • 10.1016/j.bej.2016.12.021
  9. Marques, S.; Gírio, F. M.; Santos, J. A. L.; Roseiro, J. C.. "Pulsed fed-batch strategy towards intensified process for lactic acid production using recycled paper sludge". Biomass Conversion and Biorefinery 7 2 (2016): 127-137. http://dx.doi.org/10.1007/s13399-016-0211-0.
    Published • 10.1007/s13399-016-0211-0
  10. Wu, Ming; Freitas, Sindélia; Monteiro, Gabriel; Prazeres, Duarte; Santos, José. "Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high shear vortices". Biotechnology and Applied Biochemistry 53 (2009): 237-249. http://dx.doi.org/10.1042/ba20080215.
    Published • 10.1042/ba20080215
  11. Freitas, Sindelia; Canário, Sónia; Santos, José A. L.; Prazeres, Duarte M. F.. "Alternatives for the intermediate recovery of plasmid DNA: Performance, economic viability and environmental impact". Biotechnology Journal 4 2 (2008): 265-278. http://dx.doi.org/10.1002/biot.200800216.
    Published • 10.1002/biot.200800216
  12. Marques, Susana; Santos, José A.L.; Gírio, Francisco M.; Roseiro, J. Carlos. "Lactic acid production from recycled paper sludge by simultaneous saccharification and fermentation". Biochemical Engineering Journal 41 3 (2008): 210-216. http://dx.doi.org/10.1016/j.bej.2008.04.018.
    Published • 10.1016/j.bej.2008.04.018
  13. Santos, José; Duarte Miguel Prazeres; Sindélia Freitas. "Plasmid Purification by Hydrophobic Interaction Chromatography using Sodium Citrate in the Mobile Phase". Separation and Purification Technology 65 (2008): 95-104.
    Published
  14. Santos, José; Susana Marques; Francisco M. Gírio; José Carlos Roseiro. "Lactic Acid Production from Recycled Paper Sludge". Biochemical Engineering Journal 41 (2008): 210-216.
    Published
  15. Freitas, S. S.; Azzoni, A. R.; Santos, J. A. L.; Monteiro, G. A.; Prazeres, D. M. F.. "On the stability of plasmid DNA vectors during cell culture and purification". Molecular Biotechnology 36 2 (2007): 151-158. http://dx.doi.org/10.1007/s12033-007-0028-y.
    10.1007/s12033-007-0028-y
  16. Santos, José; Duarte Miguel Prazeres; Sindélia Freitas. "Optimization of Isopropanol and Ammonium Sulfate Precipitation Steps in the Purification of Plasmid DNA". Biotechnology Progress 22 (2006): 1179-1186.
    Published
  17. Santos, José; Fernando Rodrigues Ribeiro Teles; Joaquim M.S. Cabral (AB13-9ED3-C821). "Enzymatic Degreasing of a Solid Waste from the Leather Industry by Lipases". Biotechnol. Lett. 23 (2001): 1159-1163.
    Published
  18. Belo, I.; Santos, J.A.L.; Cabral, J.M.S.; Mota, M.. "Optimization Study of Escherichia coli TB1 Cell Disruption for Cytochrome b5 Recovery in a Small-Scale Bead Mill". Biotechnology Progress 12 2 (1996): 201-204. http://dx.doi.org/10.1021/bp950085l.
    10.1021/bp950085l
  19. Santos, José; Isabel Belo; Manuel Mota; Joaquim M.S. Cabral (AB13-9ED3-C821). "Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery.". Bioseparation 6 (1996): 81-89. http://hdl.handle.net/1822/33607.
    Published
  20. Santos, J. A. L.; Cabral, J. M. S.; Cooney, C. L.. "Recovery of alkaline proteases by membrane filtration". Bioprocess Engineering 7 5 (1992): 205-211. http://dx.doi.org/10.1007/bf00369547.
    Published • 10.1007/bf00369547
  21. Santos, J. A. L.; Cabral, J. M. S.; Cooney, C. L.. "Recovery of alkaline proteases by membrane filtration". Bioprocess Engineering 7 5 (1992): 205-211. http://dx.doi.org/10.1007/bf00369547.
    10.1007/bf00369547
Thesis / Dissertation
  1. Santos, José. "Estudos de Separação de uma Proteína Recombinante por Processos de Membranas". Degree, Universidade de Lisboa Instituto Superior Técnico, 1996.
  2. Santos, José. "Recuperação de Proteases Alcalinas por Ultra-Microfiltração". Master, Universidade de Lisboa Instituto Superior Técnico, 1988.
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2006 - 2012 Valorização de Resíduos da Indústria de Reciclagem de Papel por Via Biotecnológica
Supervisor
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2004 - 2007 Development and process optimization for production and purification of plasmids for gene therapy and DNA vaccination
Co-supervisor
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1999/09 - 2000/08 Recuperação e Concentração de Proteínas Recombinadas em Larga Escala
Supervisor
Universidade de Lisboa Instituto Superior Técnico, Portugal