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Master in Biomedical Engineering by University of Minho (since 2016), pursued a scientifc carrer at Centre of Biological Engineering (CEB) since then, participating in different research projects, all related with medical biotechnology. In 2018 started her PhD in Bioengineering under the MIT-Portugal Program financed by Fundação para a Ciência e a Tecnologia. She developled her PhD activities at CEB and International Iberian Nanotechnology Laboratory (INL, Braga). Diana A. Sousa is author and co-author of a total of 5 papers in international journals with peer review , 1 book chapter, and authored 11 communications (3 oral and 8 poster) in national and international conferences.
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Identification

Personal identification

Full name
Diana Sousa

Citation names

  • Sousa, Diana A

Author identifiers

Ciência ID
7B1B-FEFA-9E0F
ORCID iD
0000-0001-5146-732X

Email addresses

  • diana.sousa@ceb.uminho.pt (Professional)

Telephones

Telephone
  • (+351) 253 604 40 (Professional)

Addresses

  • Centro de Engenharia Biológica, Campus de Gualtar, 4710-057, Braga, Braga, Portugal (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
  • Engineering and Technology - Medical Engineering - Medical Laboratory Technology
  • Engineering and Technology - Nano-technology
  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2018 - 2022
Ongoing
 Programa Doutoral em Bioengenharia (Doutoramento)
Universidade do Minho Centro de Engenharia Biológica, Portugal
"Development of targeted delivery platforms against highly metastatic cancers" (THESIS/DISSERTATION)
2011 - 2016
Concluded
 Engenharia Biomédica (Mestrado integrado)
Universidade do Minho, Portugal
"Design and validation of siRNAs against the mTOR gene towards breast cancer therapy" (THESIS/DISSERTATION)
 15
Affiliation

Science

Category
Host institution 		Employer
2016/12 - 2017/07 Researcher (Research) Universidade do Minho Centro de Engenharia Biológica, Portugal
Universidade do Minho, Portugal

Others

Category
Host institution 		Employer
2018/01 - Current PhD Student Universidade do Minho Centro de Engenharia Biológica, Portugal
Universidade do Minho, Portugal
2019/10 - 2021/12 Associated Member of the Personnel (MPA) International Iberian Nanotechnology Laboratory, Portugal
Projects

Grant

Designation Funders
2018/01 - 2022/08 Development of targeted delivery platforms against highly metastatic cancers
PD/BD/139083/2018
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
Ongoing
2017/04 - 2017/07 Circulating microRNAs as blood-based markers for breast cancer
UMINHO/BI_M/Breast_Cancer/CEB/FEV_2017
Research Fellow
Universidade do Minho Centro de Engenharia Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/12 - 2017/03 Design and validation of siRNA against the mTOR gene towards breast cancer therapy
UMINHO/BI_M/CEB
Research Fellow
Universidade do Minho Centro de Engenharia Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Sousa, Diana; Ferreira, Débora; Rodrigues, Joana L.; Rodrigues, Lígia R.. "Nanotechnology in Targeted Drug Delivery and Therapeutics". In Applications of Targeted Nano Drugs and Delivery Systems, 357-409. Elsevier, 2019.
    Published • 10.1016/b978-0-12-814029-1.00014-4
Conference abstract
  1. Sousa, Diana A; Gaspar, Ricardo; Ferreira, Celso J. O.; Baltazar, Fátima; Silva, Bruno FB; Rodrigues, Lígia R. "Cellular delivery of CRISPR/Cas9 plasmid using multivalent cationic liposomes". Paper presented in 2nd International BioDesign Research Conference, Online, 2022.
    Published
  2. Sousa, Diana A; Gaspar, Ricardo; Ferreira, Celso J. O.; Baltazar, Fátima; Silva, Bruno FB; Rodrigues, LR. "Non-viral delivery of CRISR/Cas9 for gene editing via multivalent cationic liposome system". Paper presented in XIV Spanish-Portuguese Conference on Controlled Drug Delivery, Tenerife, 2022.
    Published
  3. Sousa, Diana A; Rodrigues, Joana L; Gaspar, Ricardo; Silva, Bruno FB; Baltazar, Fátima; Rodrigues, Lígia R. "Targeting oncogenic microRNAs in triple negative breast cancer using a CRISPR/Cas9-based approach". Paper presented in 1º Workshop Online Latinoamericano en Nanobiotecnología – Aplicaciones en Agricultura, Medicina e Industria Textil, Online, 2021.
    Published
  4. Gaspar, Ricardo; Sousa, Diana A; Rodrigues, L.R; Silva, BFB. "Monitoring the association of cationic liposomes and CRISPR/Cas9 plasmid DNA with Fluorescence Cross-Correlation Spectroscopy". Paper presented in Controlled Released Society 2021 Virtual Annual Meeting, Online, 2021.
    Published
  5. Sousa, Diana A; Tsogtbaatar, Khaliunsarnai; Ferreira, Débora; Tevlek, Atakan; Aydin, Halil Murat; Çelik, Eda; Rodrigues, Lígia R. "Generation and characterization of an osteosarcoma-targeting DNA aptamer". Paper presented in 1ST Symposium Oligonucleotide Technologies & Therapeutics, Online, 2021.
    Published
  6. Ferreira, Débora; Sousa, Diana A; Silva, Cátia; Melo, Luís DR; Rodrigues, Lígia R. "Identification of highly specific aptamers towards triple negative breast cancer". Paper presented in 1ST Symposium Oligonucleotide Technologies & Therapeutics, Online, 2021.
    Published
  7. Sousa, Diana A; Rodrigues, Joana L; Baltazar, F.; Rodrigues, Ligia Raquel Marona. "In silico prediction and design of CRISPR sgRNAs targeting miR-155 for TNBC treatment". Paper presented in The 1st International BioDesign Research Conference, Online, 2020.
    Published
  8. Sousa, Diana A; Rodrigues, Joana L; Baltazar, F; Rodrigues, Ligia Raquel Marona. "CRISPR/Cas9-Mediated Knockout of miR-155 for Triple Negative Breast Cancer Treatment". Paper presented in 5th Applied Synthetic Biology in Europe, Online, 2020.
    Published
  9. Sousa, Diana A; Baltazar, F.; Rodrigues, L. R.. "Targeting oncogoenic microRNAs in triple negative breast cancer using CRISPR/Cas9 based-approach". Paper presented in MIT Portugal Annual Conference 2018, Lisboa, 2018.
  10. Sousa, Diana A; Ferreira, Débora; Silva, A.C.; Kluskens, L.D.; Rodrigues, L. R.. "Selection of a molecule that specifically targets the cell-surface Human Epidermal growth factor receptor 2: in silico docking simulation". Paper presented in BOD 2017 - Bioinformatic Open Days, Braga, 2017.
    Published
  11. Sousa, Diana A; Ferreira, Débora; Rodrigues, L. R.. "In silico selection of an ssDNA aptamer against HER2-positive breast cancer cells using computational docking simulation". Paper presented in Microbiotec'17 - Congress of Microbiology and Biotechnology 2017, Porto, 2017.
    Published
Conference poster
  1. Sousa, Diana A; Gaspar, Ricardo; Ferreira, Celso J. O.; Baltazar, Fátima; Rodrigues, Lígia R. "Cellular delivery of CRISPR/Cas9 plasmid using multivalent cationic liposomes". Paper presented in 2nd International BioDesign Research Conference, 2021.
  2. Sousa, Diana A; Tsogtbaatar, Khaliunsarnai; Ferreira, Debora; Tevlek, Atakan; Aydin, HM; Çelik, Eda; Rodrigues, Lígia R. "Generation and characterization of an osteosarcoma-targeting DNA aptamer". Paper presented in 1ST Symposium Oligonucleotide Technologies & Therapeutics, 2021.
  3. Ferreira, Debora; Sousa, Diana A; Silva, Cátia; Melo, Luís DR; Rodrigues, Ligia R. "Identification of highly specific aptamers towards triple negative breast cancer". Paper presented in 1ST Symposium Oligonucleotide Technologies & Therapeutics, 2021.
  4. Sousa, Diana A; Rodrigues, Joana L; Baltazar, F.; Rodrigues, Ligia Raquel Marona. "In silico prediction and design of CRISPR sgRNAs targeting miR-155 for TNBC treatment". Paper presented in The 1st International BioDesign Research Conference, 2020.
  5. Sousa, Diana A; Rodrigues, Joana L; Baltazar, F; Rodrigues, Ligia Raquel Marona. "CRISPR/Cas9-Mediated Knockout of miR-155 for Triple Negative Breast Cancer Treatment". Paper presented in 5th Applied Synthetic Biology in Europe, 2020.
  6. Sousa, Diana A; Baltazar, F. "Targeting oncogoenic microRNAs in triple negative breast cancer using CRISPR/Cas9 based-approach". Paper presented in 2018 MIT Portugal Annual Conference, 2018.
  7. Sousa, Diana A; Ferreira, Débora; Rodrigues, Lígia R.. "In silico selection of an ssDNA aptamer against HER2-positive breast cancer cells using computational docking simulation". Paper presented in Microbiotec'17 - Congress of Microbiology and Biotechnology 2017, 2017.
  8. Sousa, Diana A; Ferreira, Débora; Rodrigues, Lígia R.; Silva, A. C. "Selection of a molecule that specifically targets the cell-surface Human Epidermal growth factor Receptor 2: in silico docking simulation". Paper presented in BOD 2017 - Bioinformatics Open Days, 2017.
Journal article
  1. Sousa, Diana A; Carneiro, Mariana; Ferreira, Débora; Moreira, Felismina T.C.; Sales, Maria Goreti F.; Rodrigues, Lígia R.. Corresponding author: Rodrigues, Lígia R.. "Recent Advances in the Selection of Cancer-Specific Aptamers for the Development of Biosensors". Current Medicinal Chemistry 29 37 (2022): 5850-5880. http://dx.doi.org/10.2174/0929867329666220224155037.
    Published • 10.2174/0929867329666220224155037
  2. Sousa, Diana A; Gaspar, Ricardo; Ferreira, Celso J. O.; Baltazar, Fátima; Rodrigues, Ligia R.; Silva, Bruno F. B.. Corresponding author: Silva, Bruno F. B.. "In Vitro CRISPR/Cas9 Transfection and Gene-Editing Mediated by Multivalent Cationic Liposome-DNA Complexes". Pharmaceutics 14 5 (2022): 1087. http://dx.doi.org/10.3390/pharmaceutics14051087.
    Published • 10.3390/pharmaceutics14051087
  3. Pereira, Ana Cláudia; Pina, André F.; Sousa, Diana A; Ferreira, Débora; Santos-Pereira, Cátia; Rodrigues, Joana L.; Melo, Luís D. R.; et al. Corresponding author: Rodrigues, Lígia R.. "Identification of novel aptamers targeting cathepsin B-overexpressing prostate cancer cells". Molecular Systems Design & Engineering 7 6 (2022): 637-650. http://dx.doi.org/10.1039/d2me00022a.
    Published • 10.1039/d2me00022a
  4. Tsogtbaatar, Khaliunsarnai; Sousa, Diana A; Ferreira, Debora; Tevlek, Atakan; Aydin, Halil Murat; Çelik, Eda; Rodrigues, Ligia. Corresponding author: Rodrigues, Ligia. "In vitro selection of DNA aptamers against human osteosarcoma". Investigational New Drugs 40 1 (2021): 172-181. http://dx.doi.org/10.1007/s10637-021-01161-y.
    Published • 10.1007/s10637-021-01161-y
  5. Ferreira, Débora; Barbosa, Joaquim; Sousa, Diana A; Silva, Cátia; Melo, Luís D. R.; Avci-Adali, Meltem; Wendel, Hans P.; Rodrigues, Ligia R.. Corresponding author: Rodrigues, Ligia R.. "Selection of aptamers against triple negative breast cancer cells using high throughput sequencing". Scientific Reports 11 1 (2021): http://dx.doi.org/10.1038/s41598-021-87998-y.
    Published • 10.1038/s41598-021-87998-y

Intellectual property

Provisional application for patent
  1. 2021. "Isolated or synthetic DNA aptamer, methods and uses thereof".
    Protected
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/01 Non-viral delivery of CRISR/Cas9 for gene editing via multivalent cationic liposome system XIV Spanish-Portuguese Conference on Controlled Drug Delivery
(Online)
2021/07 Monitoring the association of cationic liposomes and CRISPR/Cas9 plasmid DNA with Fluorescence Cross-Correlation Spectroscopy Controlled Released Society 2021 Virtual Annual Meeting
Controlled Released Society 2021 (Online)
2020/12 Targeting oncogenic microRNAs in Triple Negative Breast Cancer using a CRISPR/Cas9-based approach 1st Workshop Latinoamericano en Nanobiotecnología
(Online)

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2021/05/09 - 2021/05/09 Lipidomics In Health & Disease
Workshop
 9th Workshop in Lipidomics
Universidade de Aveiro, Portugal
2019/06/10 - 2019/06/14 International Workshop on Innovating (IWI) (Hands-on program)
Workshop
 International Workshop on Innovating 2019
Massachusetts Institute of Technology, United States
2017/06/19 - 2017/06/20 Workshop MatLab (Process parameters and kinetics) and SuperPro (Process design and simulation with commercial software)
Workshop
 Workshop Matlab and SuperPro
Universidade do Minho, Portugal

Universidade de Vigo, Spain

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2022/02/15 Seleção in vitro de DNA aptâmero contra o osteosarcoma humano ASPIC - Associação Portuguesa de Investigação em Cancro

Mentoring / Tutoring

Topic Student name
2021/01 - 2021/12 Desenvolvimento de modelos 3D de cancro de mama para avaliação de agentes anticancerigenos Ana Rita Caldas Martins
Distinctions

Award

2021 Third prize award for poster presentation at the 2nd International BioDesign Research Conference

Other distinction

2019 Selection for participation in the International Workshop on Innovating (IWI) at Massachusetts Institute of Technology
Massachusetts Institute of Technology, United States