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Identification

Personal identification

Full name
Cristina Maria da Costa Peixoto Lisboa

Author identifiers

Ciência ID
721B-A48E-DA1B
ORCID iD
0000-0002-0995-6637

Email addresses

  • peixoto@ibet.pt (Professional)
Education
Degree Classification
2009/02/09
Concluded
 Ciências da Engenharia e Tecnologia (Doutoramento)
Major in Biotechnology
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1993/09/30
Concluded
 Química Aplicada (Licenciatura)
Major in Biotechnology
FCT-UNL, Portugal
Affiliation

Science

Category
Host institution 		Employer
2015 - Current Researcher (Research) iNOVA4Health, Portugal
iNOVA4Health, Portugal
1993 - 1995 Research Trainee (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Others

Category
Host institution 		Employer
2021/01/01 - Current Coordinator of Bioproduction Unit Instituto de Biologia Experimental e Tecnológica, Portugal
Instituto de Biologia Experimental e Tecnológica, Portugal
2009/03/01 - Current Head of DSP Development Lab, Animal Cell technology Unit Instituto de Biologia Experimental e Tecnológica, Portugal
1996/01/01 - 2008/02/28 Researcher at IBET Instituto de Biologia Experimental e Tecnológica, Portugal
Projects

Grant

Designation Funders
2021 - Current AAV PRocess INTensification with 3D printed materials
EXPL/EQU-EQU/1567/2021
Researcher
Ongoing
2020/10 - Current ADDovenom: Novel Snakebite Therapy Platform ofUnparalleled Efficacy, Safety and Affordability, 899670
899670
Researcher
European Commission Third Framework Programme
Ongoing
2020 - Current TRANSVAC-DS, Design Study for a European Vaccine, Infrastructure
951668
Researcher
Concluded
2019 - 2023 CRUZIVAX
815418
Researcher
Concluded

Contract

Designation Funders
2021 - Current Downstream Process Development of AAVs, Sartorius, Contract research services
SART2021
Principal investigator
Ongoing
2020/05 - Current Downstream Process Development of a new GT target, CSL Behring, Research contract service
CSL2BE
Principal investigator
Instituto de Biologia Experimental e Tecnológica, Portugal
2023/01/01 - 2025/12/31 Acelerar a fabricação de vesículas extracelulares derivadas de células estaminais pluripotentes humanas para regeneração cardíaca
2022.10467.PTDC
10.54499/2022.10467.PTDC
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/01 - 2025/12/31 Life Sciences for a Healthy and Sustainable Future
LA/P/0087/2020
Universidade Nova de Lisboa Microbiologia Molecular Estrutural e Celular, Portugal

Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal

Fundação Calouste Gulbenkian, Portugal

Instituto Gulbenkian de Ciência, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

BioResources 4 Sustainability, Portugal

Universidade NOVA de Lisboa, Portugal

iNOVA4Health, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022 - 2023 Characterization of viral vectors, Vector Biopharma
VBHPLC
Principal investigator
Instituto de Biologia Experimental e Tecnológica, Portugal
Concluded
2022 - 2023 Bioprocess development of viral vector, University of Munique
TUM2022
Principal investigator
Instituto de Biologia Experimental e Tecnológica, Portugal
Concluded
2021 - 2022 Production of rec Proteins, Pharmis
Ph2021
Principal investigator
Concluded
2021 - 2021 Production of viral vector, Repligen
REP2021
Researcher
Concluded
2018 - 2021 Accelerating lentivirus manufacturing: an enginnering driven approach
PTDC/EQU-EPQ/29306/2017
PTDC/EQU-EPQ/29306/2017
Principal investigator
Instituto de Biologia Experimental e Tecnológica, Portugal
 N/A
2017/05/01 - 2020/12/31 New structured substrates for downstream processing of complex biopharmaceuticals
M-ERA-NET2/0015/2016
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019/01/01 - 2019/12/31 iNOVA4Health - Programa de Medicina Translacional iBET, CEDOC/FCM, IPOLFG e ITQB
UID/Multi/04462/2019
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019 - 2019 Process Development for Turnstone Biologics
TBIO2019
Researcher
Concluded
2017 - 2019 Viral vectors Bioprocess, GELifesciences, Contract research service
GE2018
Principal investigator
Concluded
2014/05/01 - 2017/04/30 Identificação de novos bioterapêuticos e desenvolvimento de sistemas de expressão
ERA-IB-2/0007/2013
Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2017 Bioprocess development of OV - Tilt, Contract research service
TILT 2016
Principal investigator
2016 - 2017 DSP development for OV - VCN, Contract research service
VCN2016
Principal investigator
Concluded
2013 - 2017 Combinatorial immunization strategy to educate the immune system towards cross recognition and coverage against antigenic drift in seasonal influenza virus exposure
info:eu-repo/grantAgreement/EC/FP7/602640/EU
Researcher
Concluded
2014 - 2016 Enveloped VLP production & purification, Merck Millipore, Contract research service
Merck2014
Principal investigator
Concluded
2012 - 2016 Viral Capture and Purification System: Smart macroporous structures for the affinity purification of retroviral particles
info:eu-repo/grantAgreement/FCT/3599-PPCDT/118317/PT
Researcher
2015 - 2015 Standard virus - Purification, Bioreliance, Contract research service
BIOREL2015
Principal investigator
Concluded
2013 - 2015 Combining siRNA and AAV therapy approaches to target human basal-like breast cancer: from vector development to anti-tumor efficacy evaluation.
info:eu-repo/grantAgreement/FCT/3599-PPCDT/126222/PT
Researcher
Concluded
2012/03/01 - 2015 Probing adenovirus-host cell interactions: a systems biotechnology approach to improve production of gene delivery vectors
info:eu-repo/grantAgreement/FCT/3599-PPCDT/119501/PT
Researcher
Universidade do Algarve Faculdade de Ciências e Tecnologia
2012 - 2015 Enhanced membrane technology project - Sartorius Stedim, Contract research service
Sartorius2012
Principal investigator
2013/07/01 - 2014/09/30 Aceleração da purificação de biofármacos complexos através de novos processos cromatográficos, técnicas analíticas avançadas e ferramentas computacionais de desenho processual
EXPL/BBB-EBB/0790/2012
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2013/07/01 - 2014 Development of a scalable strategy for stem cells purification
info:eu-repo/grantAgreement/FCT/3599-PPCDT/128543/PT
Principal investigator
Concluded
2010 - 2014 Retroviral like particles: improving potential as candidate vaccines for Hepatitis C
info:eu-repo/grantAgreement/FCT/3599-PPCDT/102649/PT
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
2013 - 2013 DSP development for gene therapy vectors - Pall Lifescience, Research contract project
Pall 2013
Principal investigator
2012 - 2013 Debottlenecking the purification of complex biopharmaceuticals with new chromatographic processes, advanced analytics, and computer-aided process design tools
info:eu-repo/grantAgreement/FCT/3599-PPCDT/127521/PT
Researcher
2011 - 2013 Recombinant protein production and purification, BayerSchering Pharma AG, Contract research service
Bayer2011/2013
Researcher
Concluded
2010 - 2013 hCE-2 - expression and characterization in in vitro and in silico models
info:eu-repo/grantAgreement/FCT/3599-PPCDT/111530/PT
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
Concluded
2010 - 2013 2D fluorometry: a powerful tool to improve mammalian cell process development
PTDC/EBB-BIO/111530/2009
Researcher
Concluded
2011/01/01 - 2012/12/31 Strategic Project - LA 4 - 2011-2012
PEst-OE/EQB/LA0004/2011
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal

Fundação Calouste Gulbenkian, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade NOVA de Lisboa NOVA Medical School, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011 - 2012 Theraptech Project - Alloksys Life Science BV
Theraptech2011
Principal investigator
Concluded
2011 - 2012 Viral vectors for gene therapy (MGVS), Contract research service
MGVS2011
Principal investigator
Concluded
2008 - 2011 Non-human adenovirus vectors for gene teraphy transfer to brain - BRAINCAV
FP7
Researcher
Instituto de Biologia Experimental e Tecnológica, Portugal
2007/10/01 - 2010/09/30 Purificação de vírus com envelope: racionalização e aplicação de fundamentos teóricos visando a integração de processos
PTDC/EQU-EQU/71645/2006
Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2007 - 2010 Manufacturing Canine Adenovirus Vectors for Gene Therapy: development of MDCK CAV-2 E1-complementing cell lines.
info:eu-repo/grantAgreement/FCT/3599-PPCDT/69452/PT
Researcher
2006 - 2009 BACULOGENE - Baculovirus Vectors for Gene Therapy
EU (FP6 - 2007)
Technical development
Instituto de Biologia Experimental e Tecnológica, Portugal
2005/05/01 - 2008/06/30 THERAVECT - Production, purification and storage of retroviral vectors produced by modular master producer cells: focus on CoIVII vectors for the Epidermolysis Bullosa therapy
POCI/V.5/A0003/2005
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2004 - 2006 Integrated strategy for the production, Purification and storage of Adenovírus
POCTI/BIO/46515/2002
Technical development
Instituto de Biologia Experimental e Tecnológica, Portugal
Concluded
2003 - 2005 Novel strategies for a safe Rotavirus vaccine
EU (QLRT-2001-01249).
Researcher
Concluded
2001 - 2004 Mass production of Cowdria ruminantium for a cost effective veterinary vaccine aaginst heartwater
EC 8 ACP TPS 040
Researcher
Concluded

Other

Designation Funders
2021/01/01 - 2022/12/30 Improvement of chromatographic separation for AAVs and recProteins, Bio-Rad Contract research service
BR2021
Principal investigator
Concluded
Outputs

Publications

Book chapter
  1. Peixoto, Cristina; Fernandes, Rita ; Nestola, Piergiuseppe. "Downstream processing of viral-based vaccines". In Bioprocessing of Viral Vaccines. 2022.
    Published • 10.1201/9781003229797
  2. Silva, Ricardo J. S.; Mendes, João P.; Carrondo, Manuel J. T.; Marques, Paula M.; Peixoto, Cristina. "Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method". In Methods in Molecular Biology, 367-384. Springer US, 2019.
    10.1007/978-1-0716-0191-4_21
Journal article
  1. Fernandes, Rita P.; Ruiz, Afonso B.; Bezemer, Sandra; Detmers, Frank; Hermans, Pim; Peixoto, Cristina. "Targeted isolation of extracellular vesicles from cell culture supernatant using immuno-affinity chromatography". Separation and Purification Technology 358 (2025): 130312. https://doi.org/10.1016/j.seppur.2024.130312.
    10.1016/j.seppur.2024.130312
  2. Fernandes, Rita P.; Göbel, Sven; Reiter, Manfred; Bryan, Alexander; Altomonte, Jennifer; Genzel, Yvonne; Peixoto, Cristina. "Streamlining the purification of a clinical-grade oncolytic virus for therapeutic applications". Separation and Purification Technology 354 (2025): 128769. https://doi.org/10.1016/j.seppur.2024.128769.
    10.1016/j.seppur.2024.128769
  3. André Nascimento; Tiago Q. Faria; Franziska Bollmann; Marc Noverraz; Geoffrey Pressac; António Roldão; Cristina Peixoto; Ricardo J.S. Silva. "Purification of AAV8 through a scalable two-step monolithic chromatography approach". Journal of Chromatography A (2025): https://doi.org/10.1016/j.chroma.2024.465586.
    10.1016/j.chroma.2024.465586
  4. Mendes, Sónia; Faria, Tiago Q.; Nascimento, André; Noverraz, Marc; Bollmann, Franziska; Nestola, Piergiuseppe; Roldão, António; Peixoto, Cristina; Silva, Ricardo J. S.. "Accelerated Development of AAV Purification Process Using a High-Throughput and Automated Crossflow System". Separations 11 3 (2024): 73. https://doi.org/10.3390/separations11030073.
    10.3390/separations11030073
  5. Ferreira, Mariana V.; Fernandes, Sofia; Almeida, Ana Isabel; Neto, Salomé; Mendes, João P.; Silva, Ricardo J. S.; Peixoto, Cristina; Coroadinha, Ana Sofia. "Extending AAV Packaging Cargo through Dual Co-Transduction: Efficient Protein Trans-Splicing at Low Vector Doses". International Journal of Molecular Sciences 24 13 (2023): 10524. http://dx.doi.org/10.3390/ijms241310524.
    10.3390/ijms241310524
  6. Neto, Salomé; Mendes, João P.; Santos, Susana B. Dos; Solbrand, Anita; Carrondo, Manuel J. T.; Peixoto, Cristina; Silva, Ricardo J. S.. "Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents". Frontiers in Bioengineering and Biotechnology 11 (2023): http://dx.doi.org/10.3389/fbioe.2023.1183974.
    10.3389/fbioe.2023.1183974
  7. Santos, Tiago P.D.; Fernandes, Rita P.; Ribeiro, Rui P.P.L.; Peixoto, Cristina; Mota, José P.B.. "Model-based design and optimization of GSSR chromatography for peptide purification". Digital Chemical Engineering 6 (2023): 100081. http://dx.doi.org/10.1016/j.dche.2022.100081.
    10.1016/j.dche.2022.100081
  8. Moreira, Ana Sofia; Bezemer, Sandra; Faria, Tiago Q.; Detmers, Frank; Hermans, Pim; Sierkstra, Laurens; Coroadinha, Ana Sofia; Peixoto, Cristina. "Implementation of Novel Affinity Ligand for Lentiviral Vector Purification". International Journal of Molecular Sciences 24 4 (2023): 3354. http://dx.doi.org/10.3390/ijms24043354.
    10.3390/ijms24043354
  9. Fernandes, Rita P.; Escandell, José M.; Guerreiro, Ana C. L.; Moura, Filipa; Faria, Tiago Q.; Carvalho, Sofia B.; Silva, Ricardo J. S.; Gomes-Alves, Patrícia; Peixoto, Cristina. "Assessing Multi-Attribute Characterization of Enveloped and Non-Enveloped Viral Particles by Capillary Electrophoresis". Viruses 14 11 (2022): 2539. http://dx.doi.org/10.3390/v14112539.
    10.3390/v14112539
  10. Mendes, João P.; Fernandes, Bárbara; Pineda, Earl; Kudugunti, Shashi; Bransby, Mike; Gantier, René; Peixoto, Cristina; et al. "AAV process intensification by perfusion bioreaction and integrated clarification". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.1020174.
    10.3389/fbioe.2022.1020174
  11. João P. Mendes; Magnus Bergman; Anita Solbrand; Cristina Peixoto; Manuel J. T. Carrondo; Ricardo J. S. Silva. "Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography". Pharmaceutics (2022): https://doi.org/10.3390/pharmaceutics14071346.
    10.3390/pharmaceutics14071346
  12. Carvalho, Sofia B.; Silva, Ricardo J. S.; Sousa, Marcos F. Q.; Peixoto, Cristina; Roldão, António; Carrondo, Manuel J. T.; Alves, Paula M.. "Bioanalytics for Influenza Virus-Like Particle Characterization and Process Monitoring". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.805176.
    10.3389/fbioe.2022.805176
  13. Moreira, A.S.; Faria, T.Q.; Oliveira, J.G.; Kavara, A.; Schofield, M.; Sanderson, T.; Collins, M.; et al. "Enhancing the purification of Lentiviral vectors for clinical applications". Separation and Purification Technology 274 (2021): 118598. http://dx.doi.org/10.1016/j.seppur.2021.118598.
    10.1016/j.seppur.2021.118598
  14. Sofia B. Carvalho; Cristina Peixoto; Manuel J. T. Carrondo; Ricardo J. S. Silva. "Downstream processing for influenza vaccines and candidates: An update". Biotechnology and Bioengineering (2021): https://doi.org/10.1002/bit.27803.
    10.1002/bit.27803
  15. João P. Mendes; Ricardo J. S. Silva; Mikael Berg; Linda Mathiasson; Cristina Peixoto; Paula M. Alves; Manuel J. T. Carrondo. "Oncolytic virus purification with periodic counter-current chromatography". Biotechnology and Bioengineering (2021): https://doi.org/10.1002/bit.27779.
    10.1002/bit.27779
  16. Mafalda G. Moleirinho; Sean Feast; Ana S. Moreira; Ricardo J.S. Silva; Paula M. Alves; Manuel J.T. Carrondo; Tim Huber; Conan Fee; Cristina Peixoto. "3D-printed ordered bed structures for chromatographic purification of enveloped and non-enveloped viral particles". Separation and Purification Technology (2021):
    10.1016/j.seppur.2020.117681
  17. Cristina Peixoto; Otto-Wilhelm Merten. "Biomanufacturing of Gene Therapy Vectors". Biotechnology Journal 16 1 (2021): 2000614-2000614. https://doi.org/10.1002/biot.202000614.
    10.1002/biot.202000614
  18. Peixoto, Cristina. "Baculovirus affinity removal in viral-based bioprocesses". Separation and Purification Technology (2020): http://dx.doi.org/10.1016/j.seppur.2020.116693.
    10.1016/j.seppur.2020.116693
  19. Silva, Ricardo J. S.; Moleirinho, Mafalda G.; Moreira, Ana S.; Xenopoulos, Alex; Alves, Paula M.; Carrondo, Manuel J. T.; Peixoto, Cristina. "A Flow-Through Chromatographic Strategy for Hepatitis C Virus-Like Particles Purification". Processes 8 1 (2020): 85. http://dx.doi.org/10.3390/pr8010085.
    10.3390/pr8010085
  20. Moreira AS; Silva AC; Sousa MFQ; Hagner-McWhirterc Å; Ahlénc G; Lundgren M; Coroadinha AS; et al. "Establishing Suspension Cell Cultures for Improved Manufacturing of Oncolytic Adenovirus.". Biotechnology journal (2020): http://europepmc.org/abstract/med/31950598.
    10.1002/biot.201900411
  21. Ana Sofia Moreira; David Guia Cavaco; Tiago Q. Faria; Paula M. Alves; Manuel J. T. Carrondo; Cristina Peixoto. "Advances in Lentivirus Purification". Biotechnology Journal 16 1 (2020): 2000019-2000019. https://doi.org/10.1002%2Fbiot.202000019.
    10.1002/biot.202000019
  22. Moleirinho MG; Silva RJS; Alves PM; Carrondo MJT; Peixoto C. "Current challenges in biotherapeutic particles manufacturing.". Expert opinion on biological therapy (2019): http://europepmc.org/abstract/med/31773998.
    10.1080/14712598.2020.1693541
  23. Sofia B. Carvalho; Ricardo J. S. Silva; Mafalda G. Moleirinho; Bárbara Cunha; Ana S. Moreira; Alex Xenopoulos; Paula M. Alves; Manuel J. T. Carrondo; Cristina Peixoto. "Membrane-Based Approach for the Downstream Processing of Influenza Virus-Like Particles". Biotechnology Journal (2019): 1800570-1800570. https://doi.org/10.1002/biot.201800570.
    10.1002/biot.201800570
  24. Pinto C; Silva G; Ribeiro AS; Oliveira M; Garrido M; Bandeira VS; Nascimento A; et al. "Evaluation of AAV-mediated delivery of shRNA to target basal-like breast cancer genetic vulnerabilities.". Journal of biotechnology (2019): http://europepmc.org/abstract/med/31150679.
    10.1016/j.jbiotec.2019.05.016
  25. Silva MM; Gomes-Alves P; Rosa S; Simão D; Inácio JM; Peixoto C; Serra M; Belo JA; Alves PM. "Full-length human CCBE1 production and purification: leveraging bioprocess development for high quality glycosylation attributes and functionality.". Journal of biotechnology (2018): http://europepmc.org/abstract/med/30165116.
    10.1016/j.jbiotec.2018.08.015
  26. Sofia B Carvalho; A Raquel Fortuna; Michael W Wolff; Cristina Peixoto; Paula M Alves; Udo Reichl; Manuel JT Carrondo. "Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers". Journal of Chemical Technology & Biotechnology (2018): https://doi.org/10.1002/jctb.5474.
    10.1002/jctb.5474
  27. Moleirinho MG; Rosa S; Carrondo MJT; Silva RJS; Hagner-McWhirter Å; Ahlén G; Lundgren M; Alves PM; Peixoto C. "Clinical-Grade Oncolytic Adenovirus Purification Using Polysorbate 20 as an Alternative for Cell Lysis.". Current gene therapy (2018): http://europepmc.org/abstract/med/30411681.
    10.2174/1566523218666181109141257
  28. Carvalho SB; Moreira AS; Gomes J; Carrondo MJT; Thornton DJ; Alves PM; Costa J; Peixoto C. "A detection and quantification label-free tool to speed up downstream processing of model mucins.". PLoS ONE (2018): http://europepmc.org/abstract/med/29315346.
    10.1371/journal.pone.0190974
  29. Peixoto, Cristina. "Universal label-free in-process quantification of influenza virus-like particles.". Biotechnology journal (2017): http://europepmc.org/abstract/med/28514082.
    10.1002/biot.201700031
  30. Peixoto, Cristina. "Bioprocess integration for human mesenchymal stem cells: From up to downstream processing scale-up to cell proteome characterization.". Journal of biotechnology (2017): http://europepmc.org/abstract/med/28174039.
    10.1016/j.jbiotec.2017.01.014
  31. Peixoto, Cristina. "Phosphoneurofilament heavy chain and vascular endothelial growth factor as cerebrospinal fluid biomarkers for ALS.". Amyotrophic lateral sclerosis & frontotemporal degeneration (2017): http://europepmc.org/abstract/med/27538346.
    10.1080/21678421.2016.1212894
  32. Peixoto, Cristina. "Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles.". Bioconjugate chemistry (2016): http://europepmc.org/abstract/med/27652605.
  33. Peixoto, Cristina. "Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography.". Journal of chromatography. A (2016): http://europepmc.org/abstract/med/26739915.
    10.1016/j.chroma.2015.12.052
  34. Peixoto, Cristina. "Rational development of two flowthrough purification strategies for adenovirus type 5 and retro virus-like particles.". Journal of chromatography. A (2015): http://europepmc.org/abstract/med/26643723.
    10.1016/j.chroma.2015.11.037
  35. Peixoto, Cristina. "Microdosing of a Carbon-14 Labeled Protein in Healthy Volunteers Accurately Predicts Its Pharmacokinetics at Therapeutic Dosages.". Clinical pharmacology and therapeutics (2015): http://europepmc.org/abstract/med/25869840.
    10.1002/cpt.131
  36. Nestola, P.; Peixoto, C.; Silva, R.R.J.S.; Alves, P.M.; Mota, J.P.B.; Carrondo, M.J.T.. "Improved virus purification processes for vaccines and gene therapy". Biotechnology and Bioengineering 112 5 (2015): 843-857. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925689348&partnerID=MN8TOARS.
    10.1002/bit.25545
  37. Cunha, B.; Peixoto, C.; Silva, M.M.; Carrondo, M.J.T.; Serra, M.; Alves, P.M.. "Filtration methodologies for the clarification and concentration of human mesenchymal stem cells". Journal of Membrane Science 478 (2015): 117-129. http://www.scopus.com/inward/record.url?eid=2-s2.0-84921482513&partnerID=MN8TOARS.
    10.1016/j.memsci.2014.12.041
  38. Fernandes, C.S.M.; Goncçalves, B.; Sousa, M.; Martins, D.L.; Barroso, T.; Pina, A.S.; Peixoto, C.; Aguiar-Ricardo, A.; Roque, A.C.A.. "Biobased monoliths for adenovirus purification". ACS Applied Materials and Interfaces 7 12 (2015): 6605-6612. http://www.scopus.com/inward/record.url?eid=2-s2.0-84926328457&partnerID=MN8TOARS.
    10.1021/am508907b
  39. Peixoto, Cristina. "Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures.". Biomolecules (2015): http://europepmc.org/abstract/med/26248080.
    10.3390/biom5031741
  40. Nestola, P.; Silva, R.J.S.; Peixoto, C.; Alves, P.M.; Carrondo, M.J.T.; Mota, J.P.B.. "Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography". Journal of Chromatography A 1347 (2014): 111-121. http://www.scopus.com/inward/record.url?eid=2-s2.0-84901014122&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.04.079
  41. Nestola, P.; Villain, L.; Peixoto, C.; Martins, D.L.; Alves, P.M.; Carrondo, M.J.T.; Mota, J.P.B.. "Impact of grafting on the design of new membrane adsorbers for adenovirus purification". Journal of Biotechnology 181 (2014): 1-11. http://www.scopus.com/inward/record.url?eid=2-s2.0-84899647051&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2014.04.003
  42. Cunha, B.; Aguiar, T.; Silva, M.M.; Silva, R.J.S.; Sousa, M.F.Q.; Pineda, E.; Peixoto, C.; et al. "Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells". Journal of Biotechnology (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84925014987&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2015.02.023
  43. Nestola, P.; Silva, R.J.S.; Peixoto, C.; Alves, P.M.; Carrondo, M.J.T.; Mota, J.P.B.. "Robust design of adenovirus purification by two-column, simulated moving-bed, size-exclusion chromatography". Journal of Biotechnology (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84925426455&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2015.01.030
  44. Nestola, P.; Martins, D.L.; Peixoto, C.; Roederstein, S.; Schleuss, T.; Alves, P.M.; Mota, J.P.B.; Carrondo, M.J.T.. "Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration". PLoS ONE 9 12 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84920070336&partnerID=MN8TOARS.
    10.1371/journal.pone.0115802
  45. Patrone M; Carinhas N; Sousa MQ; Peixoto C; Ciferri C; Carfì A; Alves PM. "Enhanced expression of full-length human cytomegalovirus fusion protein in non-swelling baculovirus-infected cells with a minimal fed-batch strategy.". (2014): http://europepmc.org/abstract/med/24595278.
    10.1371/journal.pone.0090753
  46. Cunha B; Serra M; Peixoto C; Silva M; Carrondo M; Alves P. "Designing clinical-grade integrated strategies for the downstream processing of human mesenchymal stem cells.". BMC Proceedings (2013): http://europepmc.org/abstract/PMC/PMC3981622.
    10.1186/1753-6561-7-S6-P103
  47. Lamego, J.; Cunha, B.; Peixoto, C.; Sousa, M.F.; Alves, P.M.; Simplício, A.L.; Coroadinha, A.S.. "Carboxylesterase 2 production and characterization in human cells: New insights into enzyme oligomerization and activity". Applied Microbiology and Biotechnology 97 3 (2013): 1161-1173. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874116579&partnerID=MN8TOARS.
    10.1007/s00253-012-3994-3
  48. Fernandes, P.; Peixoto, C.; Santiago, V.M.; Kremer, E.J.; Coroadinha, A.S.; Alves, P.M.. "Bioprocess development for canine adenovirus type 2 vectors". Gene Therapy 20 4 (2013): 353-360. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875922065&partnerID=MN8TOARS.
    10.1038/gt.2012.52
  49. Bandeira, V.; Peixoto, C.; Rodrigues, A.F.; Cruz, P.E.; Alves, P.M.; Coroadinha, A.S.; Carrondo, M.J.T.. "Downstream processing of lentiviral vectors: Releasing bottlenecks". Human Gene Therapy Methods 23 4 (2012): 255-263. http://www.scopus.com/inward/record.url?eid=2-s2.0-84872231059&partnerID=MN8TOARS.
    10.1089/hgtb.2012.059
  50. Vicente, T.; Mota, J.P.B.; Peixoto, C.; Alves, P.M.; Carrondo, M.J.T.. "Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: Current advances". Biotechnology Advances 29 6 (2011): 869-878. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053444587&partnerID=MN8TOARS.
    10.1016/j.biotechadv.2011.07.004
  51. Vicente, T.; Roldão, A.; Peixoto, C.; Carrondo, M.J.T.; Alves, P.M.. "Large-scale production and purification of VLP-based vaccines". Journal of Invertebrate Pathology 107 SUPPL. (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79960620240&partnerID=MN8TOARS.
    10.1016/j.jip.2011.05.004
  52. Roldão, A.; Vicente, T.; Peixoto, C.; Carrondo, M.J.T.; Alves, P.M.. "Quality control and analytical methods for baculovirus-based products". Journal of Invertebrate Pathology 107 SUPPL. (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79960636554&partnerID=MN8TOARS.
    10.1016/j.jip.2011.05.009
  53. Lesch, H.P.; Laitinen, A.; Peixoto, C.; Vicente, T.; Makkonen, K.-E.; Laitinen, L.; Pikkarainen, J.T.; et al. "Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors". Gene Therapy 18 6 (2011): 531-538. http://www.scopus.com/inward/record.url?eid=2-s2.0-79958259186&partnerID=MN8TOARS.
    10.1038/gt.2010.162
  54. Vicente, T.; Peixoto, C.; Mota, J.P.B.; Carrondo, M.J.T.; Alves, P.M.. "Optimizing downstream processing of enveloped viruses". Genetic Engineering and Biotechnology News 31 2 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-78751503446&partnerID=MN8TOARS.
  55. Vieira, H.L.A.; Pereira, A.C.P.; Peixoto, C.C.; Moraes, R.H.P.; Alves, P.M.; Mendonça, R.Z.. "Improvement of recombinant protein production by an anti-apoptotic protein from hemolymph of Lonomia obliqua". Cytotechnology 62 6 (2010): 547-555. http://www.scopus.com/inward/record.url?eid=2-s2.0-78651509076&partnerID=MN8TOARS.
    10.1007/s10616-010-9305-x
  56. Vicente, T.; Mota, J.P.B.; Peixoto, C.; Alves, P.M.; Carrondo, M.J.T.. "Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance". Journal of Biotechnology 148 4 (2010): 171-181. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955054815&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2010.05.005
  57. Vicente, T.; Peixoto, C.; Alves, P.M.; Carrondo, M.J.T.. "Modeling electrostatic interactions of baculovirus vectors for ion-exchange process development". Journal of Chromatography A 1217 24 (2010): 3754-3764. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953022811&partnerID=MN8TOARS.
    10.1016/j.chroma.2010.03.059
  58. Vicente, T.; Mota, J.P.B.; Peixoto, C.; Alves, P.M.; Carrondo, M.J.T.. "Modeling protein binding and elution over a chromatographic surface probed by surface plasmon resonance". Journal of Chromatography A 1217 13 (2010): 2032-2041. http://www.scopus.com/inward/record.url?eid=2-s2.0-77349088483&partnerID=MN8TOARS.
    10.1016/j.chroma.2010.01.068
  59. Silva, A.C.; Peixoto, C.; Lucas, T.; Küppers, C.; Cruz, P.E.; Alves, P.M.; Kochanek, S.. "Adenovirus Vector Production and Purification". Current Gene Therapy 10 6 (2010): 437-455. http://www.scopus.com/inward/record.url?eid=2-s2.0-78651330960&partnerID=MN8TOARS.
    10.2174/156652310793797694
  60. Vicente, T.; Peixoto, C.; Carrondo, M.J.T.; Alves, P.M.; Vicente, Tiago; Peixoto, Cristina; Carrondo, Manuel J.T.; Alves, Paula M.. "Virus production for clinical gene therapy". Methods in Molecular Biology 542 (2009): 447-470. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650340776&partnerID=MN8TOARS.
    10.1007/978-1-59745-561-9_24
  61. Vicente, T.; Peixoto, C.; Carrondo, M.J.T.; Alves, P.M.. "Purification of recombinant baculoviruses for gene therapy using membrane processes". Gene Therapy 16 6 (2009): 766-775. http://www.scopus.com/inward/record.url?eid=2-s2.0-67449098368&partnerID=MN8TOARS.
    10.1038/gt.2009.33
  62. Vicente, T.; Sousa, M.F.Q.; Peixoto, C.; Mota, J.P.B.; Alves, P.M.; Carrondo, M.J.T.. "Anion-exchange membrane chromatography for purification of rotavirus-like particles". Journal of Membrane Science 311 1-2 (2008): 270-283. http://www.scopus.com/inward/record.url?eid=2-s2.0-39149107782&partnerID=MN8TOARS.
    10.1016/j.memsci.2007.12.021
  63. Peixoto, C.; Ferreira, T.B.; Sousa, M.F.Q.; Carrondo, M.J.T.; Alves, P.M.. "Towards purification of adenoviral vectors based on membrane technology". Biotechnology Progress 24 6 (2008): 1290-1296. http://www.scopus.com/inward/record.url?eid=2-s2.0-62249100590&partnerID=MN8TOARS.
    10.1002/btpr.25
  64. Mellado, M.C.M.; Peixoto, C.; Cruz, P.E.; Carrondo, M.J.T.; Alves, P.M.. "Purification of recombinant rotavirus VP7 glycoprotein for the study of in vitro rotavirus-like particles assembly". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 874 1-2 (2008): 89-94. http://www.scopus.com/inward/record.url?eid=2-s2.0-53249097321&partnerID=MN8TOARS.
    10.1016/j.jchromb.2008.09.002
  65. Peixoto, C.; Marcelino, I.; Amaral, A.I.; Carrondo, M.J.T.; Alves, P.M.. "Purification by membrane technology of an intracellular Ehrlichia ruminantium candidate vaccine against heartwater". Process Biochemistry 42 7 (2007): 1084-1089. http://www.scopus.com/inward/record.url?eid=2-s2.0-34347244554&partnerID=MN8TOARS.
    10.1016/j.procbio.2007.04.012
  66. Gouveia, R.M.; Morais, V.A.; Peixoto, C.; Sousa, M.; Regalla, M.; Alves, P.M.; Costa, J.. "Production and purification of functional truncated soluble forms of human recombinant L1 cell adhesion glycoprotein from Spodoptera frugiperda Sf9 cells". Protein Expression and Purification 52 1 (2007): 182-193. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845956245&partnerID=MN8TOARS.
    10.1016/j.pep.2006.10.008
  67. Bento, I.; Peixoto, C.; Zaitsev, V.N.; Lindley, P.F.. "Ceruloplasmin revisited: Structural and functional roles of various metal cation-binding sites". Acta Crystallographica Section D: Biological Crystallography 63 2 (2007): 240-248. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846678076&partnerID=MN8TOARS.
    10.1107/S090744490604947X
  68. Peixoto, C.; Sousa, M.F.Q.; Silva, A.C.; Carrondo, M.J.T.; Alves, P.M.. "Downstream processing of triple layered rotavirus like particles". Journal of Biotechnology 127 3 (2007): 452-461. http://www.scopus.com/inward/record.url?eid=2-s2.0-34247139971&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2006.08.002
  69. Peixoto, C.; Ferreira, T.B.; Carrondo, M.J.T.; Cruz, P.E.; Alves, P.M.. "Purification of adenoviral vectors using expanded bed chromatography". Journal of Virological Methods 132 1-2 (2006): 121-126. http://www.scopus.com/inward/record.url?eid=2-s2.0-32344445016&partnerID=MN8TOARS.
    10.1016/j.jviromet.2005.10.002
  70. Vieira, H.L.A.; Estêvão, C.; Roldão, A.; Peixoto, C.C.; Sousa, M.F.Q.; Cruz, P.E.; Carrondo, M.J.T.; Alves, P.M.. "Triple layered rotavirus VLP production: Kinetics of vector replication, mRNA stability and recombinant protein production". Journal of Biotechnology 120 1 (2005): 72-82. http://www.scopus.com/inward/record.url?eid=2-s2.0-25644448228&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2005.03.026
  71. Peixoto, C.C.; Marcelino, I.; Vachiéry, N.; Bensaid, A.; Martinez, D.; Carrondo, M.J.T.; Alves, P.M.. "Quantification of Ehrlichia ruminantium by real time PCR". Veterinary Microbiology 107 3-4 (2005): 273-278. http://www.scopus.com/inward/record.url?eid=2-s2.0-18044398537&partnerID=MN8TOARS.
    10.1016/j.vetmic.2005.02.001
  72. Souza, A.P.B.; Peixoto, C.C.; Maranga, L.; Carvalhal, A.V.; Moraes, R.H.P.; Mendonça, R.M.Z.; Pereira, C.A.; Carrondo, M.J.T.; Mendonça, R.Z.. "Purification and characterization of an anti-apoptotic protein isolated from Lonomia obliqua hemolymph". Biotechnology Progress 21 1 (2005): 99-105. http://www.scopus.com/inward/record.url?eid=2-s2.0-13544271820&partnerID=MN8TOARS.
    10.1021/bp049831p
  73. Carmo, M.; Peixoto, C.; Coroadinha, A.S.; Alves, P.M.; Cruz, P.E.; Carrondo, M.J.T.. "Quantitation of MLV-based retroviral vectors using real-time RT-PCR". Journal of Virological Methods 119 2 (2004): 115-119. http://www.scopus.com/inward/record.url?eid=2-s2.0-2442638707&partnerID=MN8TOARS.
    10.1016/j.jviromet.2004.03.007
  74. Maranga, L.; Mendonça, R.Z.; Bengala, A.; Peixoto, C.C.; Moraes, R.H.P.; Pereira, C.A.; Carrondo, M.J.T.. "Enhancement of Sf-9 cell growth and longevity through supplementation of culture medium with hemolymph". Biotechnology Progress 19 1 (2003): 58-63. http://www.scopus.com/inward/record.url?eid=2-s2.0-0347297510&partnerID=MN8TOARS.
    10.1021/bp025583q
  75. Cruz, H.J.; Conradt, H.S.; Dunker, R.; Peixoto, C.M.; Cunha, A.E.; Thomaz, M.; Burger, C.; et al. "Process development of a recombinant antibody/interleukin-2 fusion protein expressed in protein-free medium by BHK cells". Journal of Biotechnology 96 2 (2002): 169-183. http://www.scopus.com/inward/record.url?eid=2-s2.0-18444382663&partnerID=MN8TOARS.
    10.1016/S0168-1656(02)00028-7
  76. Cruz HJ; Peixoto CM; Nimtz M; Alves PM; Dias EM; Moreira JL; Carrondo MJ; et al. "Metabolic shifts do not influence the glycosylation patterns of a recombinant fusion protein expressed in BHK cells.". Biotechnology and bioengineering 69 2 (2000): 129-139. http://europepmc.org/abstract/med/10861392.
    10.1002/(sici)1097-0290(20000720)69:2<129::aid-bit2>3.3.co;2-x
  77. Cruz, P.E.; Peixoto, C.C.; Devos, K.; Moreira, J.L.; Saman, E.; Carrondo, M.J.T.. "Characterization and downstream processing of HIV-1 core and virus-like- particles produced in serum free medium". Enzyme and Microbial Technology 26 1 (2000): 61-70. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033957760&partnerID=MN8TOARS.
    10.1016/S0141-0229(99)00128-3
  78. Alves, P.M.; Fonseca, L.L.; Peixoto, C.C.; Almeida, A.C.; Carrondo, M.J.T.; Santos, H.. "NMR studies on energy metabolism of immobilized primary neurons and astrocytes during hypoxia, ischemia and hypoglycemia". NMR in Biomedicine 13 8 (2000): 438-448. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034493853&partnerID=MN8TOARS.
    10.1002/nbm.665
  79. Matias, P.M.; Donner, P.; Coelho, R.; Thomaz, M.; Peixoto, C.; Macedo, S.; Otto, N.; et al. "Structural evidence for ligand specificity in the binding domain of the human androgen receptor: Implications for pathogenic gene mutations". Journal of Biological Chemistry 275 34 (2000): 26164-26171. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034714276&partnerID=MN8TOARS.
    10.1074/jbc.M004571200
  80. Cruz, H.J.; Dias, E.M.; Peixoto, C.M.; Moreira, J.L.; Carrondo, M.J.T.. "Product quality of a recombinant fusion protein expressed in immobilised baby hamster kidney cells grown in protein-free medium". Biotechnology Letters 22 8 (2000): 677-682. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034059758&partnerID=MN8TOARS.
    10.1023/A:1005631602517
  81. Cruz, P.E.; Martins, P.C.; Alves, P.M.; Peixoto, C.C.; Santos, H.; Moreira, J.L.; Carrondo, M.J.T.. "Proteolytic activity in infected and noninfected insect cells: Degradation of HIV-1 Pr55gag particles". Biotechnology and Bioengineering 65 2 (1999): 133-143. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033589370&partnerID=MN8TOARS.
    10.1002/(SICI)1097-0290(19991020)65:2<133::AID-BIT2>3.0.CO;2-X
  82. Cruz PE; Cunha A; Peixoto CC; Clemente J; Moreira JL; Carrondo MJ; Cruz, P.E.; et al. "Optimization of the production of virus-like particles in insect cells.". Biotechnology and bioengineering 60 4 (1998): 408-418. http://europepmc.org/abstract/med/10099446.
    10.1002/(sici)1097-0290(19981120)60:4<408::aid-bit2>3.3.co;2-c
  83. Cruz, P.E.; Peixoto, C.C.; Moreira, J.L.; Carrondo, M.J.T.. "Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells". Journal of Chemical Technology and Biotechnology 72 2 (1998): 149-158. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032102575&partnerID=MN8TOARS.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/10 (R)Evolution in Downstream Processing of New Modalities: What Will be the Role of Chromatography?  SPICA International Symposium on Preparative and Industrial Chromatography and Allied Techniques
(Milan, Italy)
2023/01 Challenges and Trends in Lentivirus Manufacturing Sartorius Internal webinar
Sartorius
2022/10/14 Progress and Trends of Virus Based Biopharmaceuticals Purification SPICA International Symposium on Preparative and Industrial Chromatography and Allied Techniques
2022 Strategies and Advances in Purification of New Therapeutic Modalities Bioprocess Summit 2022
(Barcelona)
2021/10/20 Advances in Purification of Emerging Therapeutic Modalities SPICA International Symposium on Preparative and Industrial Chromatography and Allied Techniques
2021/05/03 Exploring new strategies to improve viral vectors purification processes Art of Gene Therapy - Merck
2020/07 Advance Purification of Biotherapeutic particles: Exploiting new materials and operation modes Bioprocess Summit 2020
2019 Improving downstream process of influenza virus-like particles using a pseudo-affinity strategy Bioprocess Summit 2019
(Lisbon, Portugal)
2018 Improving oncolytic adenovirus manufacturing: designing GMP compatible bioprocesses ISPPP
(Berlin, Germany)
2018 Improving downstream process of influenza virus-like particles using a pseudo-affinity strategy Bioprocess Summit
(Boston, United States)
2018 Leveraging vectored vaccine candidates manufacturing to GMP compatible bioprocess Vaccine Technology VI
(Mont Tremblant, Canada)
2018 Improving Influenza VLP vaccine purification: the effect of novel analytical tools ESBES
(Lisbon, Portugal)
2018 Challenges in Purifying Influenza Virus-Like Particles Bioprocess Summit
(Lisbon, Portugal)
2017 Improving purification of influenza virus-like particles using a pseudo-affinity strategy Affinity
(Paris, France)
2017 Facing the challenges of downstream process of new biopharmaceuticals MicroBiotec
(Porto, Portugal)
2017 Improving downstream process of new biopharmaceuticals using non - traditional chromatographic strategies Anything but Conventional Chromatography
(Lisbon, Portugal)
2016 Overcome DSP challenges UPS&DSP Technology Forum Sartorius
(Gottingen, Germany)
2016 Improving and Monitoring Influenza VLPs Downstream Process using a click chemistry approach ISBioTech 5th Spring Meeting
(Washington DC, United States)
2016 Purification of Stem Cells • SPICA 16th International Symposium on Preparative and Industrial Chromatography and Allied Techniques
(Vienna, Austria)
2016 Quality control of viral vectors, trends and overview • Universitat Autònoma de Barcelona, Centre de Biotecnologia Animal i Teràpia Gènica (CBATEG)
(Barcelona, Spain)
2015 Universal label-free in-process analytical tool for Influenza VLPs quantification Affinity
(Puerto Vallarta, Mexico)
2015 Quality control of viral vectors-overview of characterization methods Workshop EMBO
(Evry, France)
2014 Vaccines and Virus Purification Merck Millipore workshop
(Boston, United States)
2013 New insights in virus purification using enhanced membrane technology Downstream Technology Forum - Sartorius
(Gottingen, Germany)
2012 Facing the challenges of downstream process of virus Bioproduction
(Berlin, Germany)
2012 Downstream Process of virus: Overcoming bottlenecks MSSS2012
(Portoroz, Slovenia)
2009 Towards purification of baculovirus vectors using membrane technology Bioprocess International Conference
(Dusseldorf, Germany)
2008 Downstream Process of Complex Biopharmaceuticlas at IBET AKTA User day - GELifesciences
(Oeiras, Portugal)
2008 Towards purification of gene therapy vectors using membrane technology 4th European Downstream Technology Forum
(Gottingen, Germany)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2024/01/01 - Current Improving lentiviral vector bioprocessing using an engineering approach
Supervisor of Cláudia Paiva
2021 - 2025/02/14 An engineering driven approach for purification of enveloped virus-based biotherapeutics
Supervisor of Rita Patricio Fernandes
2024 - 2024 Implementation of Raman PAT Technology in tangential flow filtration to enhance downstream processing in gene therapy
Supervisor
 Master Chemical Engineering (Master)
2023 - 2024 Novel Affinity-Based Technologies for Targeting Distinct Bio-logical Systems
Supervisor
 Master Biotechnology
2022/09/01 - 2023/11/27 Accelerating complex biopharmaceuticals manufacturing
Supervisor of Afonso Ruiz
 Master Biotechnology (Master)
2022 - 2022 Accelerating Virus-Based Biopharmaceuticals Manufacturing
Supervisor
 Master (Master)
2022 - 2022 Establishment of an insect cell-based platform for production of adenovirus-like particles (ADDomer particles) as a snakebite therapy
Co-supervisor
2018 - 2022 Integrated continuous manufacturing of adeno-associated virus (AAVs)
Supervisor of João Mendes
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018 - 2022 Accelerating Lentivirus manufacturing: an engineering driven approach
Supervisor of Ana Sofia Moreira
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2020/09/01 - 2021/11/30 Get moving enveloped virus manufacturing to the clinic
Supervisor of Mariana Cordeiro de Oliveira Vinagre
2020/06/01 - 2021/01/06 Debottlenecking lentivirus manufacturing
Supervisor of David Guia Cavaco
2018 - 2019 Developing and Improving Purification Strategies for New Biopharmaceuticals
Supervisor of Rita Fernandes
2016 - 2019 Advancing purification of viral targets
Supervisor of Mafalda Moleirinho
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014 - 2018 Downstream strategies for VLPs as candidate universal vaccine for Influenza
Co-supervisor of Sofia B. Carvalho
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014 - 2018 Development of integrated strategies for the downstream processing of human stem cells for clinical application
Co-supervisor of Barbara Cunha
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014 - 2015 Advances in DSP of enveloped VLPs
Supervisor of Mafalda Moleirinho
2010 - 2014 Improving Downstream processing for viral vectors and viral vaccines
Co-supervisor
2011 - 2011 Development of virus downstream process for gene therapy
Supervisor of Patricia Roch
2011 - 2011 Purification of Complex Biopharmaceuticals with New Chromatographic Processes, Advanced Analytics, and Computer-Aided Process Design Tools
Supervisor of Duarte Martins
2007 - 2010 Downstream Processing Development of Enveloped Viruses for Clinical Applications: InnovativeTools for Rational Process Optimization
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2003 - 2008 Purification of Retroviral Vectors for Gene Therapy
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2023/11/17 - 2023/11/19 Session Chair - 24th Planova Workshop, Florence
Workshop
 24th Planova™ Workshop Florence
2022/10 - 2022/10 Session Chair - Continuous manufacturing of emerging therapeutic modalities, Integrated Continuous Biomanufacturing V, ECI Conference
Conference
 Integrated Continuous Biomanufacturing V

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2021/01/24 Bioprocess improvement for manufacturing of recombinant adeno-associated virus-based gene delivery vectors
(Thesis) Main arguer
 Pranavkumar Rajendraprasad JOSHI (PhD)
McGill University, Canada
2021/01/13 Artificial scaffolds for affinity-triggered bioseparations
(Thesis) Arguer
 Manuel Matos (PhD)
FCT-UNL, Portugal
2020/04/06 Development of a novel purification platform for Fab antibody fragments based on affinity peptides and multimodal ligands
(Thesis) Main arguer
 André Nascimento (PhD)
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
2020 Single-Column Chromatography with Recycle Lag Analog to Simulated Moving Bed Processes
(Thesis) Arguer
 Abimaelle Silva Chibério (PhD)
FCT-UNL, Portugal
2015 Implementation of a Technology Transfer System
(Thesis) Main arguer
 Ana Isabel Alves (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2013 Analysis and Optimization of the Limiting Cases of Simulated Countercurrent Adsorption:TrueMoving Bed and Single-Column Analog Processes
(Thesis) Arguer
 Ricardo Silva (PhD)
FCT-UNL, Portugal
2013 Functional monolithic platforms for antibody purification
(Thesis) Main arguer
 Telma Godinho Barroso Maciel Fernandes (PhD)
FCT-UNL, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2016 - Current Downstream Processing of Viral Vectors. Cell Culture-based Viral Vaccines - ESACT Course, Llafranc, Spain.
2010 - Current Purification of biopharmaceuticals Ciências de Engenharia Química e Bioquímica (Licenciatura) FCT-UNL, Portugal
2014 - 2018 Purification and quality control of gene therapy vectors (Doutoramento)
2010 - 2015 Downstream Process of Biopharmaceuticals (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2011 Animal Cell Technology: Biopharmaceuticals production Tecnicos Farmacia