Manoj Kumar Mandal (7219-8D5B-BFAB)

Curriculum Vitae

Manoj Kumar Mandal

I have completed the Mestrado in Bologna Master Degree (Microbiology) in 2019/11/29 by Universidade de Lisboa Instituto Superior Técnico, Lisbon, Portugal; Postgraduate Certificate in Master of Science (Applied Biomedical Science) in 2014/06/18 by University of Greenwich , London, UK, and Bachelor in Bachelor of Science (Applied Biotechnology) in 2011/07/08 by Sikkim Manipal University, Gangtok, Sikkim. Worked as Visiting Researcher in Universidade de Lisboa Instituto de Investigação do Medicamento. Worked at Associação para o Desenvolvimento do Ensino e Investigação da Microbiologia(ADEIM). Works in the area(s) of Biological Sciences with emphasis on Immunology and Microbiology, particularly on infectious diseases. Recently, worked as Research trainee at McGill University, Montreal, Canada. Currently, Phd fellow (Grant from FCT), working on the proposed research on infectious diseases, including Mtb and HIV.

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Identification

Personal identification

Full name: Manoj Kumar Mandal

Citation names

Mandal, M

Author identifiers

Ciência ID: 7219-8D5B-BFAB
ORCID iD: 0000-0003-3997-9618
Scopus Author Id: 57222736661

Email addresses

mmandal@ff.ulisboa.pt (Professional)
manoj2biomedical@gmail.com (Personal)

Telephones

Mobile phone

912567699 (Personal)

Addresses

Rua Conde Nova Goa 1, 3ESQ, 1070-071, Lisboa, Lisbon, Portugal (Personal)

Knowledge fields

Natural sciences - Biological Sciences - Microbiology
Natural sciences - Biological Sciences - Molecular Biology
Medical and Health Sciences - Basic Medicine - Immunology

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Nepali (Mother tongue)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
Portuguese	Intermediate (B1)	Upper intermediate (B2)	Intermediate (B1)	Intermediate (B1)	Intermediate (B1)

Education

	Degree	Classification
2021/11/01 - 2025/11/01 Ongoing	PhD in Pharmacy (Doutoramento) Major in Microbiology and Immunology Universidade de Lisboa Faculdade de Farmácia, Portugal
2017/02/18 - 2019/11/29 Concluded	Bologna Master Degree (Mestrado) Major in Microbiology Universidade de Lisboa Instituto Superior Técnico, Portugal "Contribution to the identification of surface exposed immunogenic proteins in bacteria from burkholderia cepacia complex" (THESIS/DISSERTATION)	16 (Very Good: 16/20)
2016/11/07 - 2016/11/15 Concluded	Training course: Scientific Writing & Publication (Outros) Universidade de Lisboa Instituto Superior Técnico, Portugal	Approved (1.5 ECTS)
2013/02/18 - 2014/06/18 Concluded	Master of Science (Postgraduate Certificate) Major in Applied Biomedical Science University of Greenwich Faculty of Engineering and Science, United Kingdom "Potential New Treatments for cutaneous Leishmaniasis" (THESIS/DISSERTATION)	Pass
2008/08/06 - 2011/07/08 Concluded	Bachelor of Science (Bachelor) Major in Applied Biotechnology Sikkim Manipal University Directorate of Distance Education, India	17 (A: Excellent, 17/20)

Affiliation

Science

	Category Host institution	Employer
2021/11/01 - Current	Researcher (Research)	Universidade de Lisboa Faculdade de Farmácia, Portugal
	Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2024/05/01 - 2024/10/31	Research Trainee (Research)	McGill University, Canada
	McGill University, Canada
2021/02/01 - 2021/10/31	Researcher (Research)	Associação para o Desenvolvimento do Ensino eInvestigação da Microbiologia, Portugal
	Universidade de Lisboa Faculdade de Farmácia, Portugal
2019/12/09 - 2021/10/30	Visiting Researcher (Research)	Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
	Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2018/04/18 - 2019/09/08	Research Trainee (Research)	Universidade de Lisboa Instituto Superior Técnico, Portugal
	Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/07/31 - 2017/09/29	Research Trainee (Research)	Carl von Ossietzky Universität Oldenburg, Germany
	Carl von Ossietzky Universität Oldenburg Institut für Chemie und Biologie des Meeres, Germany

Projects

Grant

	Designation	Funders
2021/11/01 - Current	“Developing host-directed-therapies for Mycobacterium tuberculosis infection and HIV co-infection based on Cystatins manipulation 2021.07978.BD PhD Student Fellow Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2017/07/31 - 2017/09/29	Virus Dynamics Are Influenced by Season, Tides and Advective Transport in Intertidal, Permeable Sediments VA 492/4-1 and EN 1141/1-1 Researcher Carl von Ossietzky Universität Oldenburg Institut für Chemie und Biologie des Meeres, Germany	Deutsche Forschungsgemeinschaft Concluded

Contract

	Designation	Funders
2021/02/01 - 2021/10/30	Diagnosis of infections by SARS-CoV-2/COVID-19 BI 2020/006 Research Fellow Universidade de Lisboa Faculdade de Farmácia, Portugal	Ongoing

Outputs

Publications

Conference abstract	Mandal, M; Pires, David; ANES, ELSA. "Development and characterisation of a 3D cell culture model of the tuberculosis granuloma". Paper presented in 18th International Congress of Immunology (IUIS 2023), Cape town, 2024. Accepted Mandal, MK; Seixas, AMM; Leitão, JH; Rodríguez Ortega, MJ. "Surface-shaving approaches to identify surface-exposed and immunogenic proteins from Burkholderia cenocepacia live cells.". Paper presented in 30th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), Paris, 2019. Accepted
Journal article	Manoj Mandal; David Pires; José Miguel Azevedo-Pereira; Elsa Anes. "Host-Directed Therapies Based on Protease Inhibitors to Control Mycobacterium tuberculosis and HIV Coinfection". Microorganisms (2025): https://doi.org/10.3390/microorganisms13051040. 10.3390/microorganisms13051040 Manoj Mandal; David Pires; Marta Calado; José Miguel Azevedo-Pereira; Elsa Anes. "Cystatin F Depletion in Mycobacterium tuberculosis-Infected Macrophages Improves Cathepsin C/Granzyme B-Driven Cytotoxic Effects on HIV-Infected Cells during Coinfection". International Journal of Molecular Sciences (2024): https://doi.org/10.3390/ijms25158141. 10.3390/ijms25158141 Manoj Mandal; David Pires; Maria João Catalão; José Miguel Azevedo-Pereira; Elsa Anes. "Modulation of Cystatin F in Human Macrophages Impacts Cathepsin-Driven Killing of Multidrug-Resistant Mycobacterium tuberculosis". Microorganisms (2023): https://doi.org/10.3390/microorganisms11071861. 10.3390/microorganisms11071861 Anes, Elsa; Pires, David; Mandal, Manoj; Azevedo-Pereira, José Miguel. "ESAT-6 a Major Virulence Factor of Mycobacterium tuberculosis". Biomolecules 13 6 (2023): 968. http://dx.doi.org/10.3390/biom13060968. 10.3390/biom13060968 Mandal, M; Pires, David; Matos, Ana I.; Peres, Carina; Catalão, Maria João; Azevedo-Pereira, José Miguel; Satchi-Fainaro, Ronit; Florindo, Helena F.; Anes, Elsa. "Development of Chitosan Particles Loaded with siRNA for Cystatin C to Control Intracellular Drug-Resistant Mycobacterium tuberculosis". Antibiotics 12 4 (2023): 729. http://dx.doi.org/10.3390/antibiotics12040729. 10.3390/antibiotics12040729 José Miguel Azevedo-Pereira; David Pires; Marta Calado; Manoj Mandal; Quirina Santos-Costa; Elsa Anes. "HIV/Mtb Co-Infection: From the Amplification of Disease Pathogenesis to an “Emerging Syndemic”". Microorganisms (2023): https://doi.org/10.3390/microorganisms11040853. 10.3390/microorganisms11040853 Manoj Mandal; David Pires; Jacinta Pinho; Maria João Catalão; António José Almeida; José Miguel Azevedo-Pereira; Maria Manuela Gaspar; Elsa Anes. "Liposomal Delivery of Saquinavir to Macrophages Overcomes Cathepsin Blockade by Mycobacterium tuberculosis and Helps Control the Phagosomal Replicative Niches". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms24021142. 10.3390/ijms24021142 Anes, Elsa; Pires, David; Mandal, Manoj; Azevedo-Pereira, José Miguel. "Spatial localization of cathepsins: Implications in immune activation and resolution during infections". Frontiers in Immunology 13 (2022): http://dx.doi.org/10.3389/fimmu.2022.955407. 10.3389/fimmu.2022.955407 Pires, David; Valente, Sofia; Calado, Marta; Mandal, Manoj; Azevedo-Pereira, José Miguel; Anes, Elsa. "Repurposing Saquinavir for Host-Directed Therapy to Control Mycobacterium Tuberculosis Infection". Frontiers in Immunology 12 (2021): http://dx.doi.org/10.3389/fimmu.2021.647728. Published • 10.3389/fimmu.2021.647728 Anes, Elsa; Pires, David; Velez, Tomás; Azevedo-Pereira, JM. "Modulation of Cystatin C in human macrophages improves anti-mycobacterial immune responses to Mycobacterium tuberculosis infection and co-infection with HIV.". Frontiers in Immunology (2021): Open access • Published • 10.3389/fimmu.2021.742822 Sousa, Sílvia A.; Seixas, António M.M.; Mandal, Manoj; Rodríguez-Ortega, Manuel J.; Leitão, Jorge H.. "Characterization of the Burkholderia cenocepacia J2315 Surface-Exposed Immunoproteome". Vaccines 8 3 (2020): 509. http://dx.doi.org/10.3390/vaccines8030509. Open access • Published • 10.3390/vaccines8030509
Preprint	Mandal, Manoj; Pires, David; Azevedo-Pereira, José Miguel; Anes, Elsa. "Host Directed Therapies Based on Protease Inhibitors to Control Mycobacterium Tuberculosis and HIV Coinfection". 2025. https://doi.org/10.20944/preprints202503.1286.v1. 10.20944/preprints202503.1286.v1 Cátia Silveiro; Diana Mortinho; Francisco Olivença; Manoj Mandal; David Pires; Elsa Anes; Maria João Catalão. "Effects of CwlM, a peptidoglycan synthesis regulator, on beta-lactam resistance and host-pathogen interactions". 2025. https://doi.org/10.1101/2025.07.08.663695. 10.1101/2025.07.08.663695 Anes, Elsa; Pires, David; Mandal, Manoj; Azevedo-Pereira, José Miguel. "ESAT-6 a Major Virulence Factor of Mycobacterium tuberculosis and a Target for New Therapeutic Interventions". 2023. http://dx.doi.org/10.20944/preprints202305.0979.v2. 10.20944/preprints202305.0979.v2
Thesis / Dissertation	Mandal, Manoj. "Contribution to the identification of surface-exposed immunogenic proteins of bactéria from the Burkholderia cepacia complex.". Master, Universidade de Lisboa Instituto Superior Técnico, 2019. https://fenix.tecnico.ulisboa.pt/downloadFile/1689244997259884.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2025/07/01	Cystatin F Depletion in Mycobacterium tuberculosis-Infected Macrophages Improves Cathepsin C/Granzyme B-driven Cytotoxic Effects on HIV-Infected Cells during Coinfection at XVI iMed.ULisboa Postgraduate Students Meeting	University of Lisbon (Lisbon, Portugal)
2025/06/22	Chitosan-particle-based cystatin F RNA silencing as a therapeutic strategy to improve the control of intracellular Mycobacterium tuberculosis.	The 45th ANNUAL CONGRESS of the European Society of Mycobacteriology (Lisbon, Portugal)
2025/04/11	Chitosan-particle-based cystatin F RNA silencing as a therapeutic strategy to improve the control of intracellular Mycobacterium tuberculosis presented at ESCMID 2025, Vienna, Austria	ESCMID 2025 (Vienna, Austria)
2024/11/22	Cystatin F Depletion in Mycobacterium tuberculosis-Infected Macrophages Improves Cathepsin C/Granzyme B-Driven Cytotoxic Effects on HIV-Infected Cells during Coinfection	University of Lisbon (Lisbon, Portugal)
2022/07/04	Manipulation of protease inhibitors for developing therapeutic strategies against Mycobacterium tuberculosis infection	University of Lisbon (Lisbon, Portugal)
2021/10/19	An alternative treatment to Pseudomonas aeruginosa chronic lung infection: Lytic Phage-based therapy using an exosome-liposome hybrid encapsulation during Hybrid Course Novel Challenges in Toxicology	Hybrid Course Novel Challenges in Toxicology Faculty of Pharmacy, Universidade de Lisboa (Lisbon, Portugal)
2017/08/25	Microbial ActivitiesIn Marine Sediments (Viral production in tidal permeable sediments of the Wadden Sea)	IAESTE Student Group Presentation Institute for Chemistry and Biology of the Marine Environment (ICBM)- Team Paleomicrobiology (Oldenberg, Germany)

Event participation

	Activity description Type of event	Event name Institution / Organization
2025/06/12 - 2025/06/13	The course will provide advanced training in vaccinating adult HIV patients, including insight into most recent development in the field and state of the art knowledge. Current vaccination guidelines will be reviewed and practical training in preparing individual vaccination schedule for adult patients with HIV will take place. Workshop	Give it a Shot! Vaccination in HIV Patients, Warsaw, Poland
2024/11/22 - 2024/11/22	Presented poster on "Cystatin F Depletion in Mycobacterium tuberculosis-Infected Macrophages Improves Cathepsin C/Granzyme B-Driven Cytotoxic Effects on HIV-Infected Cells during Coinfection" Conference
2024/07/11 - 2024/07/12	Presented Poster on "Cystatin F Depletion in Mycobacterium tuberculosis-Infected Macrophages Improves Cathepsin C/Granzyme B-driven Cytotoxic Effects on HIV-Infected Cells during Coinfection " Meeting	Associação da Faculdade de Farmácia para a Investigação e Desenvolvimento, Portugal
2023/06/27 - 2023/06/27	Presented Poster “Development of Chitosan Particles Loaded with siRNA for Cystatin C to Control Intracellular Drug-Resistant Mycobacterium tuberculosis” at the “Jornadas Científicas da Universidade de Lisboa 2023 Conference	Universidade de Lisboa, Portugal
2023/06/26 - 2023/06/26	Presented poster Development of Chitosan Particles Loaded with siRNA for Cystatin C to Control Intracellular Drug-Resistant Mycobacterium tuberculosis presented at the iMed.Ulisboa Meeting 2023 Meeting	Universidade de Lisboa Faculdade de Farmácia, Portugal
2023/03/31 - 2023/04/01	Presented poster Liposomal delivery of repurposed antiviral drug saquinavir to macrophages as a host-directed therapy for tuberculosis at the iMed.Ulisboa Meeting 2023. Conference
2023/03/31 - 2023/04/01	Presented poster Liposomal delivery of repurposed antiviral drug saquinavir to macrophages as a host-directed therapy for tuberculosis presented at CIIS NATIONAL MEETING: Interdisciplinary Health Care 2023, Faculdade de Medicina da Universidade Católica Portuguesa, Portugal. Meeting
2023/02/27 - 2023/03/01	Active participation in the conference "Vaccine Development-the pre- and post- COVID-19 era", Pasteur Institute, France, Paris Conference	"Vaccine Development"-the pre- and post- COVID-19 era, Pasteur Institute, France, Paris Institut Pasteur, France
2022/09/12 - 2022/09/16	Participated and presented poster Developing therapeutic strategies against Mycobacterium tuberculosis infection based on manipulation of protease inhibitors at the EMBO workshop on Tuberculosis 2022. Congress	EMBO workshop on Tuberculosis 2022 (Paris, France)
2022/05/16 - 2022/05/18	Presented poster Repurposing Saquinavir for host-directed therapy to control Mycobacterium tuberculosis infection at the annual meeting of science, technology and innovation in Portugal Ciência 2022 Conference	The annual meeting of science, technology and innovation in Portugal Ciência 2022
2021/09/17 - 2021/09/24	Workshop for flow cytometry analysis Workshop	iMed Workshop on FCS Express for flow cytometry analysis 2021 Universidade de Lisboa Faculdade de Farmácia, Portugal
2021/06/16 - 2021/07/09	Presented Poster entitled "Repurposing Saquinavir for host-directed therapy to control Mycobacterium Tuberculosis Infection. Meeting	iMed.Ulisboa Meeting 2021 Universidade de Lisboa Faculdade de Farmácia, Portugal
2020/01/31 - 2020/01/31	Member of the group (Host-Pathogen Interactions Unit, iMed-Ulisboa) presenting at II Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal 31 de janeiro de 2020 Conference	II Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal Universidade de Lisboa Faculdade de Farmácia, Portugal
2019/11/27 - 2019/11/27	Member of the group (Group de Ciências Biológicas, Centro de Engenharia Biológica e Química, IST, Lisboa) presenting at 30th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Conference	30th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)

Committee member

	Activity description Role	Institution / Organization
2010/03/04 - 2012/09/17	Motivated for promotion of biotechnology in Nepal. Member	Biotechnology Society of Nepal, Nepal

Distinctions

Award

2023	Best poster award at CIIS NATIONAL MEETING: Interdisciplinary Health Care 2023 by Faculdade de Medicina da Universidade Católica Portuguesa, Portugal.