Bruno Adriano Sousa Silva (6E10-32B5-C862)

Curriculum Vitae

Bruno Adriano Sousa Silva

Bruno Adriano Sousa Silva completed the PhD in Molecular Biology in 2013/12/13 by Universidade de Lisboa Faculdade de Ciências, Masters in Molecular Biology and Genetics in 2008/11/10 by Universidade de Lisboa Faculdade de Ciências and Bachelor in Microbiology and Genetics in 2007/09 by Universidade de Lisboa Faculdade de Ciências. Is Postdoc in Instituto de Medicina Molecular. Published 15 articles in journals (6 as first author and 4 as a co-first author). Participated in 11 events. Co-supervised 6 MSc dissertations. Supervised 2 work of course completion of LSc/BSc. Has received 2 awards and/or honors. Participated as a Research Fellow in 1 project, Postdoc Fellow in 1 project. Currently participates as Principal investigator in 2 projects and as co-Principal investigator in 1 project where he supervises or co-supervises 1 postdocs, 1 PhD student and 2 technicians. Works in the area(s) of Medical and Health Sciences with emphasis on Basic Medicine and Biological Sciences with emphasis on Molecular Biology. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: ALT cancers, Telomeres, Genome stability, Iron metabolism, Hereditary Hemochromatosis, HFE, Alternative Lengthening of Telomeres, Replication Stress and SUB1.

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Identification

Personal identification

Full name: Bruno Adriano Sousa Silva

Citation names

Silva, Bruno

Author identifiers

Ciência ID: 6E10-32B5-C862
ORCID iD: 0000-0002-1452-9779
Scopus Author Id: 57209021272

Knowledge fields

Medical and Health Sciences - Basic Medicine
Natural sciences - Biological Sciences - Molecular Biology

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
French	Beginner (A1)	Intermediate (B1)	Beginner (A1)	Beginner (A1)

Education

	Degree	Classification
2013/12/13 Concluded	Doutoramento em Biologia, Especialidade Biologia Molecular (Doutoramento) Universidade de Lisboa Faculdade de Ciências, Portugal "The functional link between HFE and hepcidin - its contribution to iron metabolism regulation" (THESIS/DISSERTATION)	Excellent (approved with distinction and honors)
2008/11/10 Concluded	Biologia Molecular e Genética (Mestrado) Universidade de Lisboa Faculdade de Ciências, Portugal "ESTUDO DAS CONSEQUÊNCIAS ESTRUTURAIS E FUNCIONAIS DAS ALTERAÇÕES E277K E V295A NA PROTEÍNA HFE – Esclarecimento da sua contribuição para o desenvolvimento de Hemocromatose Hereditária" (THESIS/DISSERTATION)	18 out of 20
2007/09 Concluded	Biologia Microbiana e Genética (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal "-" (THESIS/DISSERTATION)	16 out of 20

Affiliation

Science

	Category Host institution	Employer
2018/03/26 - Current	Postdoc (Research)	Instituto de Medicina Molecular, Portugal

2015/10/01 - 2018/01/31	Visiting Researcher (Research)	National Institute on Aging Intramural Research Program, United States

2013/12/14 - 2015/09/30	Researcher (Research)	Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

2009/12/01 - 2013/12/13	Research Trainee (Research)	Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

2008/12/01 - 2009/11/30	Researcher (Research)	Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

2007/09/17 - 2008/11/10	Research Trainee (Research)	Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

2006/07/01 - 2006/08/31	Research Trainee (Research)	Instituto Gulbenkian de Ciência, Portugal

Teaching in Higher Education

	Category Host institution	Employer
2010/05/01 - 2010/05/31	Lecturer (University Teacher)	Universidade de Lisboa Faculdade de Ciências, Portugal

Projects

Grant

	Designation	Funders
2021/10/01 - Current	Study of Alternative Lengthening of Telomeres-associated telomeric replicative stress alleviators - paving avenues towards innovative anti-cancer therapies PTDC/MED-ONC/7864/2020 Principal investigator Instituto de Medicina Molecular, Portugal	Fundação para a Ciência e a Tecnologia
2018/10/01 - Current	Telomeric R-loop-mediated recombination in the alternative lengthening of telomeres pathway: towards designing novel therapeutic avenues for cancer therapy PTDC/MED-ONC/28282/2017 Principal investigator Instituto de Medicina Molecular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/06/01 - Current	TERRA and telomerase-mediated telomere elongation: unveiling novel molecular pathways towards the development of innovative therapeutic approaches for cancer treatment. PTDC/BIA-MOL/29352/2017 Other Instituto de Medicina Molecular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2008/12/01 - 2009/11/30	Study of post-transcriptional regulation of HFE gene expression - a contribution to understand its role in iron homeostasis and in Hereditary Hemocromatosis development PTDC/SAU-GMG/64494/2006 Research Fellow Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2015/10/01 - 2018/01/31	SFRH/BI/106064/2015 SFRH/BI/106064/2015 Post-doc Fellow	Concluded
2009/12/01 - 2013/11/30	THE FUNCTIONAL LINK BETWEEN HFE AND HEPCIDIN - ITS CONTRIBUTION TO IRON METABOLISM REGULATION SFRH/SFRH/BD/60718/2009/PT Fundação para a Ciência e a Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Outputs

Publications

Conference abstract

Silva, Bruno. "The expression of the soluble HFE corresponding transcripts is up-regulated by intracellular iron and inhibits iron absorption in a duodenal cell model". Paper presented in The International Liver Congress 2018, Paris, 2018.
Accepted
Silva, Bruno. "Genetics of iron metabolism related gene expression and biochemical phenotype profile in peripheral blood supports iron homeostasis dysregulation in Alzheimer’s Disease". Paper presented in Bioiron 2013, London, 2013.
Published
Silva, Bruno. "Duodenal Cytochrome b and Hephaestin expression is regulated by the soluble HFE isoform". Paper presented in Bioiron 2013, London, 2013.
Published
Silva, Bruno. "Alternative Splicing Regulates HFE Gene Expression in Several Tissues". Paper presented in European Iron Club Meeting 2010, Nijmegen, 2010.
Published

Journal article

Bruno Silva; Rajika Arora; Silvia Bione; Claus M. Azzalin. "TERRA transcription destabilizes telomere integrity to initiate break-induced replication in human ALT cells". Nature Communications 12 1 (2021): https://doi.org/10.1038/s41467-021-24097-6.
10.1038/s41467-021-24097-6
Domingues-Silva, Beatriz; Silva, Bruno; Azzalin, Claus M.. "ALTernative Functions for Human FANCM at Telomeres". Frontiers in Molecular Biosciences 6 (2019): http://dx.doi.org/10.3389/fmolb.2019.00084.
10.3389/fmolb.2019.00084
Silva, Bruno; Pentz, Richard; Figueira, Ana Margarida; Arora, Rajika; Lee, Yong Woo; Hodson, Charlotte; Wischnewski, Harry; Deans, Andrew J.; Azzalin, Claus M.. "FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops". Nature Communications 10 1 (2019): http://dx.doi.org/10.1038/s41467-019-10179-z.
Published • 10.1038/s41467-019-10179-z
Silva, Bruno; Nunes, Alexandra; Vale, Filipa F.; Rocha, Raquel; Gomes, João Paulo; Dias, Ricardo; Oleastro, Mónica. "The expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretion". Helicobacter 22 4 (2017): e12390. http://dx.doi.org/10.1111/hel.12390.
10.1111/hel.12390
Lau, William W.; Sparks, Rachel; Tsang, John S.. "Meta-analysis of crowdsourced data compendia suggests pan-disease transcriptional signatures of autoimmunity". F1000Research 5 (2016): 2884. http://dx.doi.org/10.12688/f1000research.10465.1.
10.12688/f1000research.10465.1
Faria, Ricardo; Silva, Bruno; Silva, Catarina; Loureiro, Pedro; Queiroz, Ana; Fraga, Sofia; Esteves, Jorge; et al. "Next-generation sequencing of hereditary hemochromatosis-related genes: Novel likely pathogenic variants found in the Portuguese population". Blood Cells, Molecules, and Diseases 61 (2016): 10-15. http://dx.doi.org/10.1016/j.bcmd.2016.07.004.
10.1016/j.bcmd.2016.07.004
Guerreiro, Cláudia; Silva, Bruno; Crespo, Ângela C.; Marques, Liliana; Costa, Sónia; Timóteo, Ângela; Marcelino, Erica; et al. "Decrease in APP and CP mRNA expression supports impairment of iron export in Alzheimer's disease patients". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1852 10 (2015): 2116-2122. http://dx.doi.org/10.1016/j.bbadis.2015.07.017.
10.1016/j.bbadis.2015.07.017
Silva, Bruno; Faustino, Paula. "An overview of molecular basis of iron metabolism regulation and the associated pathologies". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1852 7 (2015): 1347-1359. http://dx.doi.org/10.1016/j.bbadis.2015.03.011.
10.1016/j.bbadis.2015.03.011
Silva, Bruno; Ferreira, Joana; Santos, Vera; Baldaia, Cilénia; Serejo, Fátima; Faustino, Paula. "The soluble form of HFE protein regulates hephaestin mRNA expression in the duodenum through an endocytosis-dependent mechanism". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1842 11 (2014): 2298-2305. http://dx.doi.org/10.1016/j.bbadis.2014.07.017.
10.1016/j.bbadis.2014.07.017
Crespo, Ângela C.; Silva, Bruno; Marques, Liliana; Marcelino, Erica; Maruta, Carolina; Costa, Sónia; Timóteo, Ângela; et al. "Genetic and biochemical markers in patients with Alzheimer's disease support a concerted systemic iron homeostasis dysregulation". Neurobiology of Aging 35 4 (2014): 777-785. http://dx.doi.org/10.1016/j.neurobiolaging.2013.10.078.
10.1016/j.neurobiolaging.2013.10.078
Silva, B.; Pita, L.; Gomes, S.; Gonçalves, J.; Faustino, P.. "The hepcidin gene promoter nc.-1010C > T; -582A > G haplotype modulates serum ferritin in individuals carrying the common H63D mutation in HFE gene". Annals of hematology 93 12 (2014): 2063-2066. http://www.scopus.com/inward/record.url?eid=2-s2.0-84922254973&partnerID=MN8TOARS.
Silva, Bruno; Martins, Rute; Proença, Daniela; Fleming, Rita; Faustino, Paula. "The functional significance of E277K and V295A HFE mutations". British Journal of Haematology 158 3 (2012): 399-408. http://dx.doi.org/10.1111/j.1365-2141.2012.09164.x.
10.1111/j.1365-2141.2012.09164.x
Martins, Rute; Proença, Daniela; Silva, Bruno; Barbosa, Cristina; Silva, Ana Luísa; Faustino, Paula; Romão, Luísa. "Alternative Polyadenylation and Nonsense-Mediated Decay Coordinately Regulate the Human HFE mRNA Levels". PLoS ONE 7 4 (2012): e35461. http://dx.doi.org/10.1371/journal.pone.0035461.
10.1371/journal.pone.0035461
Marques, Liliana; Auriac, Anne; Willemetz, Alexandra; Banha, João; Silva, Bruno; Canonne-Hergaux, François; Costa, Luciana. "Immune cells and hepatocytes express glycosylphosphatidylinositol-anchored ceruloplasmin at their cell surface". Blood Cells, Molecules, and Diseases 48 2 (2012): 110-120. http://dx.doi.org/10.1016/j.bcmd.2011.11.005.
10.1016/j.bcmd.2011.11.005
Martins, Rute; Silva, Bruno; Proença, Daniela; Faustino, Paula. "Differential HFE Gene Expression Is Regulated by Alternative Splicing in Human Tissues". PLoS ONE 6 3 (2011): e17542. http://dx.doi.org/10.1371/journal.pone.0017542.
10.1371/journal.pone.0017542

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2021/12/17	TERRA transcription induces telomere loss in a Mus81-dependent manner in ALT cancer cells	Telomeres & Telomerase Meeting, Cold Spring Harbor Laboratory, USA Cold Spring Harbor Laboratory, USA (Cold Spring Harbor, United States)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2022/01/03 - Current	Study of the mechanisms of recruitment of FANCM to the telomeres of ALT cancer cells Supervisor	Universidade de Lisboa Faculdade de Medicina, Portugal
2020/09/01 - 2022/01/10	Exploring the roles of SUB1 in the Alternative Lengthening of Telomeres pathway Co-supervisor	Bioquímica para a Saúde (Master) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/09/01 - 2021/01/12	Novel regulators of telomere elongation in ALT cells Co-supervisor	Master course in Molecular Biology and Genetics (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2020/01/17 - 2020/07/03	Generation of ATRX depleted telomerase-positive and primary human cell lines Supervisor	Bachelor in Biologia Celular e Molecular (Degree) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/07/15 - 2020/06/01	The roles of telR-loops in the stability of telomeres in primary and telomerase-positive cancer cells Co-supervisor	Ciências Biofarmacêuticas (Master) Universidade de Lisboa Faculdade de Farmácia, Portugal
2013/10/01 - 2014/09/30	Hemocromatose hereditária não-classica-pesquisa de mutações em genes relacionados com o metabolismo do ferro e estudos funcionais de uma nova variante da proteína hemojuvelina Co-supervisor	Análises Clínicas (Master) Universidade de Lisboa Faculdade de Farmácia, Portugal
2011/10/01 - 2012/12/13	Pesquisa e caracterização de mutações em genes relacionados com o metabolismo do ferro em individuos com hiperferritinemia grave Co-supervisor	Mestrado em Biologia Humana e Ambiente (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2009/10/01 - 2010/09/30	Contribuição para o conhecimento de factores genéticos moduladores do metabolismo do ferro Co-supervisor	Mestrado em Ciências Biomédicas (Master) Universidade dos Açores, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2019/04/30 - 2019/05/04	Poster - FANCM restricts R-loop-induced telomeric replication stress in ALT cells Conference	Telomeres & Telomerase Meeting, Cold Spring Harbor Laboratory, USA
2016/09/26 - 2016/09/30	Mechanisms of Aging Conference	Mechanisms of Aging Meeting, Cold Spring Harbor Laboratory, USA
2014/04/09 - 2014/04/13	Poster presentation - The expression of the soluble HFE corresponding transcripts is up-regulated by intracellular iron and inhibits iron absorption in a duodenal cell model. Conference	The International Liver Congress, London, UK
2013/04/14 - 2013/04/18	Poster presentation - Duodenal Cytochrome b and Hephaestin expression is regulated by the soluble HFE isoform. Poster presentation - Genetics of iron metabolism related gene expression and biochemical phenotype profile in peripheral blood supports iron homeostasis dysregulation in Alzheimer’s Disease. Conference	International BioIron Society, London, UK
2012/11/08 - 2012/11/10	Poster presentation - A soluble HFE splice variant seems to regulate the expression of duodenal cytochrome b and hephaestin contributing to iron metabolism regulation. Conference	Reunião Annual Sociedade Portuguesa de Hematologia, Porto, Portugal
2011/09/08 - 2011/09/11	Poster presentation - Variants of hepcidin gene promoter modulate iron status in H63D individuals. Poster presentation - Differential regulation of ceruloplasmin isoforms expression in macrophages and hepatocytes. Conference	European Iron Club Meeting, Louvain-la-Neuve, Belgium
2010/04/16 - 2010/04/18	Poster presentation - Could an HFE splice variant be used as a therapeutic agent in iron overload disorders? Workshop	ESH-EHA Scientific Workshop on Innovative Therapies for Red Cell and Iron Related Disorders, Cascais, Portugal
2009/11/19 - 2009/11/21	Poster presentation - Hepcidin gene promoter c.-1010T and c.-582G variants are modulators of iron overload development in individuals carrying the H63D mutation in the HFE gene Conference	13th Portuguese Society of Human Genetics Meeting, Porto, Portugal
2009/06/07 - 2009/06/11	Poster presentation - Differential expression of HFE splice variants Poster presentation - E277K and V295A HFE mutations: a matter of a3-domain location?	The Third Congress of the International BioIron Society, Porto, Portugal
2008/11/20 - 2008/11/22	Poster presentation - Structural and functional consequences of E277K and V295A mutations in the HFE protein – contribution to the development of hereditary hemochromatosis Poster presentation - How abundant are HFE transcript variants in human tissues? Conference	12ª Reunião da Sociedade Portuguesa de Genética, Lisboa, Portugal
2008/09/18 - 2008/09/19	Poster presentation - Is the HFE expression regulated by an mRNA surveillance mechanism? Poster presentation - Quantifying HFE transcript variants in human tissues. Poster presentation - Cellular trafficking of E277K and V295A HFE mutations. Conference	Annual Meeting of the European Iron Club, St Gallen, Switzerland

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2022/03/22	The impact of Lama2-deficiency on cell cycle regulation and survival (Thesis) Main arguer	Catarina Elisa Mineiro de Melo (Master) Universidade de Lisboa Faculdade de Ciências, Portugal
2021/01/15	Designing novel tools to detect and quantify non-canonical DNA structures (Thesis) Main arguer	Eduardo Luis Correia Gameiro (Master) Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/11/08	Functional dissection of the Xist lncRNA conserved sequences in X-chromosome inactivation (Thesis) Main arguer	Claudia Gil (Master) Universidade de Lisboa Faculdade de Ciências, Portugal

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2020/04/07 - 2020/04/07	Lecture in 'Role of Telomeric Transcription in Cancer and Ageing'	Biologia Molecular e Genética (Mestrado integrado)	Universidade de Lisboa Faculdade de Ciências, Portugal
2010/05/01 - 2010/05/31	Cellular regulation and differentiation	Regulação e Diferenciação (Master)	Universidade de Lisboa Faculdade de Ciências, Portugal

Mentoring / Tutoring

	Topic	Student name
2021/03/01 - Current	Decoding R-loop formation, function and relevance in cancer cells	Cristiana Marisa de Sousa Morgado

Distinctions

Other distinction

2012	Experimental work presentation - 1st prize award in 2012 - 'A soluble HFE splice variant seems to regulate the expression of duodenal cytochrome b and hephaestin contributing to iron metabolism regulation'
2011	Poster presentation 1st prize award - "Differential regulation of ceruloplasmin isoforms expression in macrophages and hepatocytes" at European Iron Club Meeting 2011, Louvain-la-Neuve, Belgium.