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Identification

Personal identification

Full name
Tiago André Afonso dos Santos
Date of birth
1990/04/30
Gender
Male

Citation names

  • Santos, Tiago

Author identifiers

Ciência ID
6517-B7D0-2B07
ORCID iD
0000-0003-4620-0174

Email addresses

  • tiagoaasantos@medicina.ulisboa.pt (Professional)

Addresses

  • Instituto de Medicina Molecular João Lobo Antunes, Edifício Egas Moniz, Avenida Professor Egas Moniz, 1649-028, Lisboa, Lisboa, Portugal (Professional)

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2023/04/17 - 2023/04/20
Concluded
 Summer School in Personalized Medicine - Multimodel Biomarkers and Diagnostics (Outros)
European Infrastructure for Translational Medicine, Netherlands
2018/06/01 - 2022/10/07
Concluded
 Biomedicina (Doutoramento)
Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
"Recognition of G-quadruplexes in microRNA precursors by nucleolin and its implications in cancer" (THESIS/DISSERTATION)
2014/09/01 - 2015/07/13
Concluded
 Biotecnologia (Mestrado)
Universidade da Beira Interior Faculdade de Ciências, Portugal
"Production and Purification of DNA G-quadruplex using pPH600 plasmid" (THESIS/DISSERTATION)
2009/09/01 - 2013/06/03
Concluded
 Biotecnologia (Licenciatura)
Universidade da Beira Interior Faculdade de Ciências, Portugal
"Vacina de DNA PEB1: uma nova abordagem para a prevenção da colonização por Campylobacter em humanos e aves" (THESIS/DISSERTATION)
Affiliation

Science

Category
Host institution 		Employer
2022/11/07 - Current Contracted Researcher (Research) VectorB2B, Portugal
Universidade de Lisboa Instituto de Medicina Molecular, Portugal
2022/11/07 - Current Researcher (Research) Universidade de Lisboa Instituto de Bioquímica, Portugal
Universidade de Lisboa Instituto de Medicina Molecular, Portugal
2014/06 - 2022/10 Researcher (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2021/06/13 - 2021/07/04 Visiting Researcher (Research) Institut Européen de Chimie et Biologie, France
Institut Européen de Chimie et Biologie, France
2020/09/04 - 2020/10/02 Visiting Researcher (Research) Institut Européen de Chimie et Biologie, France
Institut Européen de Chimie et Biologie, France
2019/09/30 - 2019/10/11 Visiting Researcher (Research) Institut Européen de Chimie et Biologie, France
Institut Européen de Chimie et Biologie, France

Teaching in Higher Education

Category
Host institution 		Employer
2016/09 - 2022/06 Tutor (University Teacher) Universidade da Beira Interior Faculdade de Ciências, Portugal
Universidade da Beira Interior Faculdade de Ciências, Portugal

Other Careers

Category
Host institution 		Employer
2022/08/25 - 2022/10/28 Técnico Superior (Técnico Superior) Universidade da Beira Interior, Portugal
Universidade da Beira Interior, Portugal

Positions / Appointments

Category
Host institution 		Employer
2016/01 - 2022/01 Manager of Laboratory, Institute, Museum, Centre or Observatory Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Projects

Grant

Designation Funders
2021/11 - 2022/10 BInov 2021 Award, Innovation Grant funded by the Southern and Autonomous Regions Regional Section (SRSRA) of the Order of Pharmacists
BInov 2021
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Ordem dos Farmaceuticos
Ongoing
2018/06 - 2022/07 Recognition of G-quadruplexes in microRNA precursors by nucleolin and its implications in cancer
PD/BD/142851/2018
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2017/10/02 - 2018/05/31 Nucleolin targeting by G-quadruplex aptamers for cervical cancer therapy
IF/00959/2015
Research Fellow
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde
Concluded
2016/10 - 2017/08 Innovative diagnosis of prostate cancer based on pre-miRNA G-quadruplexes
Ref BID/ICI-FCS/CICS/Santander Universidades-UBI/2016
Research Fellow
Universidade da Beira Interior, Portugal
 Santander Totta/UBI
Concluded

Contract

Designation Funders
2022/10 - 2022/11 Novel approach to control let-7e miRNA biogenesis: Unveiling the relationship between structure and function of let-7e precursor miRNA/nucleolin complex
APPID: 2473
Researcher
Instruct-ERIC, United Kingdom
 Instruct-ERIC
Ongoing
2022/07 - 2022/10 Vaginal gel for topical application in precancerous lesions caused by the HPV
CENTRO-01-0145-FEDER-181235
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Comissão de Coordenação e Desenvolvimento Regional do Centro
Ongoing
2021/11 - 2022/10 CombinatiOn aptaMer-drug Based therapeutics for ocular neovascular dIsease: wheN two are bettEr than one
UIDP/Multi/00709/2020
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde
Ongoing
2020/11 - 2021/12 SENSE - Deteção de eStruturas de G-quadruplEx no SARS-CoV-2 utilizando soNdas fluorescentes
ref. 6
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Universidade da Beira Interior
Concluded
2020/01 - 2021/12 Production of the full-length nucleolin for structural studies
PID: 10168
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Instruct-ERIC
Concluded
2018/09 - 2021/08 Multifunctional Nanoseeds for Chemoradiotherapy of Glioblastoma
PTDC/MED-QUI/29649/2017
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019/01 - 2020/12 Innovative strategy for cervical cancer theranostics based on G-quadruplexes of the KRAS gene
PESSOA ref. 5079/2019
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/11 - 2020/11 DREAM - DRug dElivery nAnosysteM for HPV infection therapy
UTAP-EXPL/NTec/0015/2017
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2020/05 - 2020/08 Track and Trace COVID-19
149_596826219
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/09 - 2020/02 BIODEVICE - BIOmedical DEVICE development based on pre-miRNA G-quadruplex
MIT-EXPL/BIO/0008/2017
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Cruz, Carla; Miranda, André; Santos, Tiago. "Aptamer-based microfluidics for circulating tumor cells". In Aptamers Engineered Nanocarriers for Cancer Therapy, 403-432. Elsevier, 2023.
    10.1016/b978-0-323-85881-6.00004-x
Conference paper
  1. Miranda, André; Cruz, Carla; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Jardim, Andreia; Caneira, Catarina; et al. "Microfluidic assay based on G-quadruplex aptamer for nucleolin detection in prostate cancer". Paper presented in 1st International Electronic Conference on Biosensors, 2020.
    10.3390/iecb2020-07064
Conference poster
  1. Moreira, David; Leitão, Daniela; Lopes-Nunes, Jéssica; Santos, Tiago; Figueiredo, Joana; Miranda, André; Alexandre, Daniela; et al. "G-quadruplex aptamer interactions with ligands and nucleolin". Paper presented in INTERACTION WINS 2023, 2023.
  2. Alexandre, Daniela; Santos, Tiago; Lopes-Nunes, Jéssica; Miranda, André; Figueiredo, Joana; Silva, Micael; Mergny, Jean-Louis; Batista, Pedro V.; Cruz, Carla. "Stabilization of AT11-B1 aptamer by ligand binding". Paper presented in XVII Annual Symposium CICS-UBI 2022, 2022.
  3. Santos, Tiago; Silva, Micael; Imbert, Lionel; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "RNA G-quadruplex motifs in precursor microRNAs and their interaction with small molecules and nucleolin". Paper presented in Structural Biology Conference 2022, 2022.
  4. Santos, Tiago; Miranda, André; Imbert, Lionel; Monchaud, David; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "Targeting a G-quadruplex from let-7e pre-miRNA with small molecules and nucleolin". Paper presented in Chemical biology symposium 2022 - Royal Society of Chemistry, 2022.
  5. Alexandre, Daniela; Santos, Tiago; Lopes-Nunes, Jéssica; Miranda, André; Figueiredo, Joana; Silva, Micael; Mergny, Jean-Louis; Batista, Pedro V.; Cruz, Carla. "Structural characterization of a G-quadruplex aptamer and their ligands". Paper presented in 4th National Organic Chemistry Meeting, 7th National Medicinal Chemistry Meeting, 2022.
  6. Santos, Tiago; Miranda, André; Imbert, Lionel; Monchaud, David; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "Formation of a RNA G-quadruplex in pre-let-7e and its interaction with ligands and nucleolin". Paper presented in InteractionWins 2021 - Virtual Winter School, 2021.
  7. Miranda, André; Santos, Tiago; Largy, Eric; Cruz, Carla. "Locking of AS1411 aptamer with a flanking duplex: a mechanism to reduce polymorphism?". Paper presented in InteractionWins 2021 - Virtual Winter School, 2021.
  8. Santos, Tiago; Miranda, André; Imbert, Lionel; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "Formation of a G-quadruplex by pre-miRNA-149 and its interaction with nucleolin protein". Paper presented in 1st Symposium on Oligonucleotide Technologies and Therapeutics - OTP 2021, 2021.
  9. Miranda, André; Santos, Tiago; Largy, Eric; Cruz, Carla. "Flanking duplex can be a locking mechanism of structural polymorphism of AS1411 aptamer maintaining their properties?". Paper presented in 1st Symposium on Oligonucleotide Technologies and Therapeutics@Portugal - OTP 2021, 2021.
  10. Santos, Tiago; Imbert, Lionel; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "RNA G-quadruplexes in pre-miRNAs: A new way in the target of nucleolin". Paper presented in WG2 ONLINE CA17140 COST CONFERENCE “Characterisation of nanomaterials towards safe and efficient nanodrugs”, 2021.
  11. Santos, Tiago; Miranda, André; Imbert, Lionel; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "Formation of a G-quadruplex structure by a sequence found in pre-miRNA-let-7e". Paper presented in International Symposium on SupraBiomolecular Systems, 7th edition, 2021.
  12. Miranda, André; Santos, Tiago; Largy, Eric; Cruz, Carla. "Could a dual elongation of the AS1411 aptamer sequence induce a lock on structural polymorphism and maintenance of properties?". Paper presented in International Symposium on SupraBiomolecular Systems, 7th edition, 2021.
  13. Santos, Tiago; Imbert, Lionel; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "Insights into pre-miRNA-149 G-quadruplex recognition by nucleolin: A structural study". Paper presented in 9th IberoAmerican NMR meeting/10th GERMN biennial NMR meeting, 2021.
  14. Miranda, André; Santos, Tiago; Largy, Eric; Cruz, Carla. "Lock of AS1411 polymorphism with a flanking duplex". Paper presented in 9th IberoAmerican NMR meeting/10th GERMN biennial NMR meeting, 2021.
  15. Santos, Tiago; Miranda, André; Jardim, Andreia; Caneira, Catarina R. F.; Chu, Virginia; Conde, João P.; Imbert, Lionel; et al. "pre-miR-149 G-quadruplex as a molecular recognition agent of Nucleolin". Paper presented in Applied Nanotechnology and Nanoscience International Conference - ANNIC 2021, 2021.
  16. Miranda, André; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Vaz, Virgilio; Pereira, Bruno J.; Cruz, Carla. "Nucleolina: um biomarcador clinicamente relevante em doentes com cancro da próstata?". Paper presented in 3º ENJIO - ENCONTRO NACIONAL DE JOVENS INVESTIGADORES EM ONCOLOGIA, 2021.
  17. Miranda, André; Santos, Tiago; Largy, Eric; Cruz, Carla. "Flanking duplex can be a locking mechanism of structural polymorphism of AS1411 aptamer maintaining their properties?". Paper presented in Encontro Ciência'21 - Encontro Nacional com a Ciência e a Tecnologia, 2021.
  18. Miranda, André; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Vaz, Virgilio; Pereira, Bruno J.; Chu, Virginia; Conde, João P.; Cruz, Carla. "Nucleolina: um biomarcador clinicamente relevante em doentes com cancro da próstata?". Paper presented in I Semana da Investigação do Centro Hospitalar Universitário Cova da Beira, 2020.
  19. Santos, Tiago; Miranda, André; Campello, Maria P. C.; Paulo, António; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "Sonda fluorescente de RNA G-quadruplex para diagnóstico do cancro da próstata". Paper presented in I Semana da Investigação do Centro Hospitalar Universitário Cova da Beira, 2020.
  20. Santos, Tiago; Miranda, André; Campello, Maria P. C.; Paulo, António; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "Nucleolin interaction with RNA G-quadruplex formed in the pre-miRNA-92b". Paper presented in XV Annual CICS-UBI Symposium, 2020.
  21. Miranda, André; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Jardim, Andreia; Caneira, Catarina R. F.; Vaz, Virgilio; et al. "Microfluidic assay based on G-quadruplex aptamer for nucleolin detection in prostate cancer". Paper presented in 1st International Electronic Conference on Biosensors, 2020.
  22. Miranda, André; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Jardim, Andreia; Caneira, Catarina R. F.; Vaz, Virgilio; et al. "Detection of Nucleolin Using a Microfluidic Assay Based on G Quadruplex Aptamer". Paper presented in Encontro Ciência 2020, 2020.
  23. Santos, Tiago; Pereira, Patricia; Pedro, Augusto Q.; Dias, Jorge S.; Viegas, Aldino; Queiroz, João A.; Cabrita, Eurico J.; Cruz, Carla. "Nucleolin/miR-149 complex: A strategy in the target of prostate cancer cells". Paper presented in III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer, 2019.
  24. Jardim, Andreia; Huck, Michael; Catarina R. F. Caneira; Carvalho, Josué; Santos, Tiago; Chu, Virginia; Cruz, Carla; Conde, João P.. "Detection of the potential cancer biomarker nucleolin in a microfluidic system". Paper presented in III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer, 2019.
  25. Miranda, André; Santos, Tiago; Carvalho, Josué; Chu, Virginia; Conde, João P.; Cruz, Carla. "Molecular Beacon Derived from AS1411 to Nucleolin Detection". Paper presented in III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer, 2019.
  26. Miranda, André; Santos, Tiago; Carvalho, Josué; Chu, Virginia; Conde, João P.; Cruz, Carla. "AS1411 Derived Molecular Beacon to Nucleolin Detection". Paper presented in 2nd Portuguese Symposium on Research and Innovations in Urology, 2019.
  27. Santos, Tiago; Pereira, Patricia; Pedro, Augusto Q.; Dias, Jorge S.; Viegas, Aldino; Queiroz, João A.; Cabrita, Eurico J.; Cruz, Carla. "miR-149 G-quadruplex recognition by the nucleolin protein in prostate cancer cells". Paper presented in PTNMR meeting - SMASH conference 2019, 2019.
  28. Alexandre, Daniela J.; Santos, Tiago; Carvalho, Josué; Sousa, Adriana; Pereira, Bruno J.; Sousa, Fani; Chu, Virginia; Conde, João P.; Cruz, Carla. "Nucleolin detection for diagnosis of prostate cancer". Paper presented in XIV Annual CICS-UBI Symposium, 2019.
  29. Miranda, André; Santos, Tiago; Carvalho, Josué; Queiroz, João A.; Chu, Virginia; Conde, João P.; Cruz, Carla. "G-quadruplex Aptamer Beacons for the Detection of Nucleolin". Paper presented in XIV Annual CICS-UBI Symposium, 2019.
  30. Santos, Tiago; Pereira, Patricia; Dias, Jorge S.; Viegas, Aldino; Queiroz, João A.; Cabrita, Eurico J.; Cruz, Carla. "The pre-miRNA 149 - nucleolin complex: An integrated strategy to assembly the complex for the targeting of prostate cancer cells". Paper presented in XIV Annual CICS-UBI Symposium, 2019.
  31. Santos, Tiago; Pereira, Patricia; Campello, Maria P. C.; Paulo, António; Cabrita, Eurico J.; Cruz, Carla. "Nucleolin targeting by RNA G-quadruplex of pre-miR-149". Paper presented in BIONIC 2018, 2018.
  32. Santos, Tiago; Brito, Andreia; Boto, Renato; Sousa, Pedro; Almeida, Paulo; Cruz, Carla; Tomaz, Cândida. "Purification of Influenza DNA vaccine using pHEMA cryogel support". Paper presented in ESBES 2018, 2018.
  33. Santos, Tiago; Brito, Andreia; Boto, Renato; Sousa, Pedro; Almeida, Paulo; Cruz, Carla; Tomaz, Cândida. "Purification of Influenza DNA vaccine by a pHEMA cryogel support". Paper presented in XIII Annual CICS-UBI symposium 2018, 2018.
  34. Carrilho, Rui; Santos, Tiago; Carvalho, Josué; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "Study of the interaction between a RNA G-quadruplex aptamer and ligands". Paper presented in XIII Annual CICS-UBI symposium 2018, 2018.
  35. Martins, Henrique; Santos, Tiago; Pereira, Patricia; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "Production of G-quadruplex pre-miRNA-149 and its purification by affinity chromatography". Paper presented in XIII Annual CICS-UBI symposium 2018, 2018.
  36. Santos, Tiago; Proença, Zélia; Queiroz, João A.; Tomaz, Cândida; Cruz, Carla. "Plasmid DNA purification using a naphthalene tripodal support". Paper presented in XII Annual CICS-UBI symposium 2017, 2017.
  37. Santos, Tiago; Ferreira, João; Pereira, Patricia; Corvo, Marta C.; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "Selective isolation of G-quadruplex parallel sequences with naphthalene amine affinity support". Paper presented in 6th International Meeting on Quadruplex Nucleic Acids, 2017.
  38. Santos, Tiago; Ferreira, João; Pereira, Patricia; Corvo, Marta C.; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "New affinity support to isolate G4 parallel sequences". Paper presented in II Congress in Health Sciences Research: Towards innovation and entrepreneurship 2017, 2017.
  39. Santos, Tiago; Pereira, Patricia; Corvo, Marta C.; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "Synthesis of a novel support derived from naphthalene to purify G-quadruplex structures". Paper presented in V ciclo de conferências da Faculdade de Ciências, UBI, 2017.
  40. Santos, Tiago; Pereira, Patrícia; Sousa, Fani; Queiroz, João A.; Cruz, Carla. "Purification of the supercoiled G-quadruplex pDNA for in vitro transcription". Paper presented in XVI Latin-American Congress on Chromatography and 9th National Meeting on Chromatography, 2016.
  41. Ferreira, João; Santos, Tiago; Carvalho, Josué; Pereira, Patricia; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "Purification of plasmid DNA using a chromatographic support derived from G-quadruplex ligands". Paper presented in XVI Latin-American Congress on Chromatography and 9th National Meeting on Chromatography, 2016.
  42. Tiago Santos; Patrícia Pereira; Fani Sousa; João A. Queiroz; Carla Cruz. "Purification of G-quadruplex supercoiled plasmid DNA with chromatographic supports based on amino acids". Paper presented in I Congress in Health Sciences Research: Towards innovation and entrepreneurship 2015, 2015.
Journal article
  1. Santos, Tiago; Silva, Micael; Imbert, Lionel; Campello, Maria P. C.; Paulo, António; Amrane, Samir; Salgado, Gilmar F.; Cruz, Carla; Cabrita, Eurico J. "A Structural Perspective into the Interaction of an Oncogenesis-Relevant pre-miRNA G-Quadruplex Ligand Carrier with the Protein Nucleolin". Chemistry – A European Journal (2023): http://dx.doi.org/10.1002/chem.202301181.
    10.1002/chem.202301181
  2. Moreira, David; Leitão, Daniela; Lopes-Nunes, Jéssica; Santos, Tiago; Figueiredo, Joana; Miranda, André; Alexandre, Daniela; et al. "G-Quadruplex Aptamer-Ligand Characterization". Molecules 27 20 (2022): 6781. http://dx.doi.org/10.3390/molecules27206781.
    10.3390/molecules27206781
  3. Santos, Tiago; Lopes-Nunes, Jéssica; Alexandre, Daniela; Miranda, André; Figueiredo, Joana; Silva, Micael S.; Mergny, Jean-Louis; Cruz, Carla. "Stabilization of a DNA aptamer by ligand binding". Biochimie 200 (2022): 8-18. http://dx.doi.org/10.1016/j.biochi.2022.05.002.
    10.1016/j.biochi.2022.05.002
  4. Santos, Tiago; Salgado, Gilmar F.; Cabrita, Eurico J.; Cruz, Carla. "Nucleolin: a binding partner of G-quadruplex structures". Trends in Cell Biology 32 7 (2022): 561-564. http://dx.doi.org/10.1016/j.tcb.2022.03.003.
    10.1016/j.tcb.2022.03.003
  5. Santos, Tiago; Miranda, André; Imbert, Lionel; Monchaud, David; Salgado, Gilmar F.; Cabrita, Eurico J.; Cruz, Carla. "Targeting a G-quadruplex from let-7e pre-miRNA with small molecules and nucleolin". Journal of Pharmaceutical and Biomedical Analysis 215 (2022): 114757. http://dx.doi.org/10.1016/j.jpba.2022.114757.
    10.1016/j.jpba.2022.114757
  6. Ramos, Catarina I.V.; Almodôvar, Vitor A.S.; Candeias, Nuno R.; Santos, Tiago; Cruz, Carla; Neves, M. Graça P.M.S.; Tomé, Augusto C.. "Diketopyrrolo[3,4-c]pyrrole derivative as a promising ligand for the stabilization of G-quadruplex DNA structures". Bioorganic Chemistry 122 (2022): 105703. http://dx.doi.org/10.1016/j.bioorg.2022.105703.
    10.1016/j.bioorg.2022.105703
  7. Santos, Tiago; Miranda, André; Imbert, Lionel; Jardim, Andreia; Caneira, Catarina R.F.; Chu, Virgínia; Conde, João P.; et al. "Pre-miRNA-149 G-quadruplex as a molecular agent to capture nucleolin". European Journal of Pharmaceutical Sciences 169 (2022): 106093. http://dx.doi.org/10.1016/j.ejps.2021.106093.
    10.1016/j.ejps.2021.106093
  8. Carvalho, Josué; Lopes-Nunes, Jéssica; Figueiredo, Joana; Santos, Tiago; Miranda, André; Riscado, Micaela; Sousa, Fani; et al. "Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection". Sensors 21 21 (2021): 7015. http://dx.doi.org/10.3390/s21217015.
    10.3390/s21217015
  9. Figueiredo, Joana; Santos, Tiago; Miranda, André; Alexandre, Daniela; Teixeira, Bernardo; Simões, Pedro; Lopes-Nunes, Jéssica; Cruz, Carla. "Ligands as Stabilizers of G-Quadruplexes in Non-Coding RNAs". Molecules 26 20 (2021): 6164. http://dx.doi.org/10.3390/molecules26206164.
    10.3390/molecules26206164
  10. Santos, Tiago; Salgado, Gilmar F.; Cabrita, Eurico J.; Cruz, Carla. "G-Quadruplexes and Their Ligands: Biophysical Methods to Unravel G-Quadruplex/Ligand Interactions". Pharmaceuticals 14 8 (2021): 769. http://dx.doi.org/10.3390/ph14080769.
    10.3390/ph14080769
  11. Santos, Tiago; Miranda, André; Campello, Maria P.C.; Paulo, António; Salgado, Gilmar; Cabrita, Eurico J.; Cruz, Carla. "Recognition of nucleolin through interaction with RNA G-quadruplex". Biochemical Pharmacology 189 (2021): 114208. http://dx.doi.org/10.1016/j.bcp.2020.114208.
    10.1016/j.bcp.2020.114208
  12. Miranda, André; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Jardim, Andreia; Caneira, CatarinaR.F.; Vaz, Vírgilio; et al. "Aptamer-based approaches to detect nucleolin in prostate cancer". Talanta 226 (2021): 122037. http://dx.doi.org/10.1016/j.talanta.2020.122037.
    10.1016/j.talanta.2020.122037
  13. Cruz, Carla; Pedro, Augusto Q.; Carvalho, Josué; Santos, Tiago; Talhada, Daniela; Paiva, Artur; Queiroz, João A.; et al. "Nucleolin as a potential biomarker for canine malignant neoplasia". Research in Veterinary Science 135 (2021): 297-303. http://dx.doi.org/10.1016/j.rvsc.2020.10.004.
    10.1016/j.rvsc.2020.10.004
  14. Miranda, André; Santos, Tiago; Largy, Eric; Cruz, Carla. "Locking up the AS1411 Aptamer with a Flanking Duplex: Towards an Improved Nucleolin-Targeting". Pharmaceuticals 14 2 (2021): 121. http://dx.doi.org/10.3390/ph14020121.
    10.3390/ph14020121
  15. Santos, Tiago; Pereira, Patrícia; Campello, Maria Paula Cabral; Paulo, António; Queiroz, João A.; Cabrita, Eurico; Cruz, Carla. "RNA G-quadruplex as supramolecular carrier for cancer-selective delivery". European Journal of Pharmaceutics and Biopharmaceutics 142 (2019): 473-479. http://dx.doi.org/10.1016/j.ejpb.2019.07.017.
    10.1016/j.ejpb.2019.07.017
  16. Santos, Tiago; Pereira, Patrícia; Queiroz, João A.; Cruz, Carla; Sousa, Fani. "Plasmid production and purification: An integrated experiment-based biochemistry and biotechnology laboratory course". Biochemistry and Molecular Biology Education 47 6 (2019): 638-643. http://dx.doi.org/10.1002/bmb.21290.
    10.1002/bmb.21290
  17. Carvalho, Josué; Santos, Tiago; Carrilho, Rui; Sousa, Fani; Salgado, Gilmar F.; Queiroz, João António; Cruz, Carla. "Ligand screening to pre-miRNA 149 G-quadruplex investigated by molecular dynamics". Journal of Biomolecular Structure and Dynamics 38 8 (2019): 2276-2286. http://dx.doi.org/10.1080/07391102.2019.1632743.
    10.1080/07391102.2019.1632743
  18. Santos, Tiago; Brito, Andreia; Boto, Renato; Sousa, Pedro; Almeida, Paulo; Cruz, Carla; Tomaz, Cândida. "Influenza DNA vaccine purification using pHEMA cryogel support". Separation and Purification Technology 206 (2018): 192-198. http://dx.doi.org/10.1016/j.seppur.2018.06.002.
    10.1016/j.seppur.2018.06.002
  19. Santos, T.; Proença, Z.; Queiroz, J.A.; Tomaz, C.; Cruz, C.. "Plasmid purification by using a new naphthalene tripodal support". Separation and Purification Technology 188 (2017): 81-89. http://dx.doi.org/10.1016/j.seppur.2017.06.072.
    10.1016/j.seppur.2017.06.072
  20. Ferreira, João; Santos, Tiago; Pereira, Patrícia; Corvo, Marta C.; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "Naphthalene amine support for G-quadruplex isolation". The Analyst 142 16 (2017): 2982-2994. http://dx.doi.org/10.1039/c7an00648a.
    10.1039/c7an00648a
  21. Santos, Tiago; Carvalho, Josué; Corvo, Marta C.; Cabrita, Eurico J.; Queiroz, J.A.; Cruz, C.. "l-tryptophan and dipeptide derivatives for supercoiled plasmid DNA purification". International Journal of Biological Macromolecules 87 (2016): 385-396. http://dx.doi.org/10.1016/j.ijbiomac.2016.02.079.
    10.1016/j.ijbiomac.2016.02.079
  22. Santos, Tiago; Pereira, Patrícia; Sousa, Fani; Queiroz, João A.; Cruz, Carla. "Purification of supercoiled G-quadruplex pDNA for in vitro transcription". Separation and Purification Technology 163 (2016): 59-71. http://dx.doi.org/10.1016/j.seppur.2016.02.036.
    10.1016/j.seppur.2016.02.036
Thesis / Dissertation
  1. "Recognition of G-quadruplexes in microRNA precursors by nucleolin and its implications in cancer". PhD, Universidade da Beira Interior, 2022.
  2. "Production and Purification of DNA G-quadruplex using pPH600 plasmid". Master, 2013.

Intellectual property

Provisional application for patent
  1. 2022. "Molecular detection method for SARS-CoV-2 RNA".
    Protected
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/07 Insights into the interaction of an RNA G-quadruplex with a ligand and nucleolin: A structural view XVII Annual Symposium CICS-UBI 2022
CICS-UBI - Centro de Investigação em Ciências da Saúde (Covilhã, Portugal)
2022/05 From structure to function: Insights into the interaction of an RNA G-quadruplex with a ligand and nucleolin V Ciclo de Conferências Biomédicas
BioMedUBI - Núcleo de Estudantes de Ciências Biomédicas da UBI (Covilhã, Portugal)
2022/03/11 Targeting of nucleolin/G-quadruplex partnership PCISBIO day 2022
Portuguese Centre for Integrated Structural Biology (Lisbon, Portugal)
2021/09/30 Identification and characterization of an RNA G-quadruplex as Nucleolin binder XVI Annual CICS-UBI Symposium
CICS-UBI - Centro de Investigação em Ciências da Saúde (Covilhã)
2021/07 RNA G-quadruplex: a strategy in the target of cell surface nucleolin Eucaryotic initiation factors – Meeting of ARNA
Institut Européen de Chimie et Biologie (IECB) (Bordéus, France)
2020/10 Microfluidic assay based on G-quadruplex aptamer for nucleolin detection in prostate cancer XV Annual CICS-UBI symposium 2020
CICS-UBI - Centro de Investigação em Ciências da Saúde (Covilhã, Portugal)
2019/10 G-quadruplexes nanosystems for cancer therapy 1st COST Action CA17140 Conference
(Riga, Latvia)
2018/07 Acridine orange derivatives binding to RNA G-quadruplex and potential use in nucleolin targeting XIII Annual CICS-UBI Symposium
(Covilhã, Portugal)
2018/06 Circulating nucleolin as a potential biomarker for canine tumours XXIII Meeting SPPA 2018
(Coimbra, Portugal)
2018/03 Nucleolin as target of RNA G-quadruplex aptamer for prostate cancer diagnosis 6th National Meeting of Chemistry Students
(Covilhã, Portugal)
2016/11 Innovative diagnosis of prostate cancer based on pre-miRNA G-quadruplexes 3 minutes, 1 slide, your Thesis!
(Covilhã, Portugal)
2016/07 Screening of L-tryptophan and dipeptide derivatives as ligands for affinity chromatography XI Annual CICS-UBI Symposium
(Covilhã, Portugal)
2015/07 Production and purification of DNA G-quadruplex using pPH600 plasmid X Annual CICS-UBI Symposium
(Covilhã, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/02 - 2022/06 Therapeutic approach for lung cancer: Selection and characterization of G-quadruplex RNA aptamers for the PD-L1 protein
Co-supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior, Portugal
2021/02 - 2021/06 Formação de estruturas G-quadruplex em pre-miRNAs e sua implicação no cancro
Co-supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior, Portugal
2020/02 - 2020/06 MicroRNAs circulantes em biópsias líquidas para diagnóstico do cancro da próstata
Co-supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior, Portugal
2019/02 - 2019/06 Produção e purificação da proteína nucleolina e sua utilização no diagnóstico do cancro da próstata
Co-supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior Faculdade de Ciências, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2022/06/06 - 2022/06/09 Hands-on course on NMR-based Metabolomics: principles, methods, and applications (2022/06/06 - 2022/06/09)
Workshop (Member of the Organising Committee)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2019/11/14 - 2019/11/16 III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer (2019/11/14 - 2019/11/16)
Congress (Member of the Organising Committee)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2018/07/05 - 2018/07/06 XIII Annual CICS-UBI symposium 2018 (2018/07/05 - 2018/07/06)
Symposium (Member of the Organising Committee)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2013/05/24 - 2013/05/26 Encontro Nacional de Estudantes de Biotecnologia 2013 - ENEBT13 (2013/05/24 - 2013/05/26)
Meeting (Member of the Organising Committee)
 Universidade da Beira Interior, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2021/02/04 - 2021/02/04 World Cancer Day 2021 - Round Table online with CICS-UBI Researchers
Round table
 Round Table online with CICS-UBI Researchers
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2022/06 Development of a therapeutic formulation for topical application in precancerous lesions caused by the Human Papilloma Virus
(Thesis) Main arguer
 Catarina Caetano (Other)
Universidade da Beira Interior, Portugal
2022/06 Development of hybridization probes for lung cancer therapy
(Thesis) Main arguer
 Daniela Leitão (Other)
Universidade da Beira Interior, Portugal
2021/06 G-quadruplex structures as targets in lung cancer therapy
(Thesis) Main arguer
 Catarina Guimarães (Other)
Universidade da Beira Interior, Portugal
2021/06 Development of a point-of-care device for biomarker detection in blood samples for lung carcinoma
(Thesis) Arguer
 Beatriz Araújo (Other)
Universidade da Beira Interior, Portugal
2020/06 Determination of miR-150 expression in plasma and/or serum as a potential biomarker for the diagnosis of non-small cell lung cancer
(Thesis) Main arguer
 Diana Bastos (Other)
Universidade da Beira Interior, Portugal
2019/06 6. Development of G-quadruplex RNA-based nanosystems for lung cancer therapy
(Thesis) Main arguer
 Raphael Figueira (Other)
Universidade da Beira Interior, Portugal
2016/06 Radiolabeled G-quadruplex aptamers for the detection of HPV-infected cancer cells
(Thesis) Main arguer
 Fábio Matos (Other)
Universidade da Beira Interior, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2023/02 - Current Frontiers in Bioscience-Landmark IMR Press
2022/12 - Current Annals of Clinical Microbiology and Antimicrobials BMC – Part of Springer Nature group
2022/03/24 - Current Tumor Biology SAGE Journals

Association member

Society Organization name Role
2013/01 - 2014/01 Núcleo de Estudantes de Biotecnologia da Universidade da Beira Interior (UBIOTEC) 1º Secretário

Committee member

Activity description
Role 		Institution / Organization
2018 - 2021 Comissão de Alunos de Doutoramento do Centro de Investigação em Ciências da Saúde da Universidade da Beira Interior (CAD-CICS)
Member
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2023/02 - 2023/02 Discovery and Toxicology session (Outros) VectorB2B, Portugal
2022/10 - 2022/10 Analytical Methods - Course Unit - Module of Circular Dichroism Ciências Farmacêuticas (Mestrado integrado) Universidade da Beira Interior, Portugal
2016/09 - 2022/06 Biochemistry II - Course Unit Ciências Farmacêuticas (Mestrado integrado) Universidade da Beira Interior, Portugal
2020/02 - 2020/06 Laboratory rotations - Course unit Biomedicina (Doutoramento) Universidade da Beira Interior, Portugal

Mentoring / Tutoring

Topic Student name
2021/09/06 - 2021/09/30 International Federation of Medical Students - Associations (IFMSA) Exchange Program Marina Staneva
2019/07/01 - 2019/07/31 International Federation of Medical Students’ Associations (IFMSA) Exchange Program Vanja Hrešcak
2019/07/01 - 2019/07/31 International Pharmaceutical Students - Federation (IPSF) Exchange Program Vlatkovic Milica
2019/07/01 - 2019/07/31 International Pharmaceutical Students’ Federation (IPSF) Exchange Program Emilija Krstic
2019/01/01 - 2019/01/31 Identificação da nucleolina como um potencial biomarcador tumoral do cancro da próstata Nuno Alexandre França Rua
2017/07/03 - 2017/07/28 International Federation of Medical Students’ Associations (IFMSA) Exchange Program Maha El Barch
2017/07/10 - 2017/07/14 Ciência Viva no Laboratório Ricardo Pedrosa, Beatriz Coelho, Inês Veloso

Other jury / evaluation

Activity description Institution / Organization
2020/03/24 - 2020/03/24 Bolsa de Investigação (BI), para mestre, no âmbito do Projeto Nº 022161, com a designação "Rede Nacional de Ressonância Magnética Nuclear, com a referência PINFRA/22161/2016" Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
Distinctions

Award

2022 PhD Student Fellowship - Biennial 2022 - Instruct-ERIC
Instruct-ERIC, United Kingdom
2020 Best poster presentation
Multidisciplinary Digital Publishing Institute AG, Switzerland
2018 Best oral presentation
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2016 1st place in competition "3 minutos, 1 slide... A tua Tese!"
Universidade da Beira Interior, Portugal

Banco Santander Totta, Portugal