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Daniela Barreiro has a B.Sc. in Biochemistry (2018) and a M.Sc. in Biochemistry (2020) from NOVA School of Science and Technology (FCT-NOVA). Currently enrolled in the Doctoral Program in Biochemistry at FCT-NOVA (2021-2025), Daniela is developing her PhD research project (under FCT grant) at the Microbial Stress Research Laboratory @UCIBIO with the supervision of Dr. Sofia Pauleta and co-supervision of Dr. Rita Sobral. Her research focuses on the biochemical characterization of metalloproteins, copper and hemic, proposed to be involved in the infection process of pathogenic bacteria. Daniela has a total of 12 poster communications and 2 oral presentations and 2 flash-presentations at national and international conferences, as well as 3 published articles in peer-reviewed Q1 journals. As part of a collaboration, Daniela is currently a visiting PhD Student in the Environmental Biogeochemistry Research Laboratory of Centro de Química Estrutural at Instituto Superior Técnico. Here, Daniela is performing bioelectrochemistry studies of metalloproteins under the guidance of Prof. Dr. Margarida dos Santos Romão, with part of the work developed already presented as 3 poster communication and 1 oral presentation at conferences.
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Identification

Personal identification

Full name
Daniela Sofia Colaço Barreiro

Citation names

  • Barreiro, Daniela S.

Author identifiers

Ciência ID
5F17-D842-5273
ORCID iD
0000-0002-6390-1186

Email addresses

  • d.barreiro@campus.fct.unl.pt (Professional)

Telephones

Telephone
  • 212948385 Ext.: 10937 (Professional)

Addresses

  • Dept. Química, FCT-UNL, Campus da Caparica, 2829-516, Caparica, Almada, Portugal (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Proficiency (C2) Advanced (C1) Proficiency (C2)
Portuguese (Mother tongue)
Education
Degree Classification
2021/03/01 - 2025/02/28
Ongoing
 Bioquímica (Doutoramento)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/09/09 - 2020/11/27
Concluded
 Bioquímica (Mestrado)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Biochemical characterization of cytochrome c2 and nitrite reductase from Neisseria gonorrhoeae Grade: 19/20" (THESIS/DISSERTATION)
 18
2015/09/21 - 2018/06/21
Concluded
 Bioquímica (Licenciatura)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Isolation and Biochemical Characterization of Multicopper Oxidase of Staphylococcus aureus" (THESIS/DISSERTATION)
 15
Affiliation

Science

Category
Host institution 		Employer
2019/09/01 - Current Researcher (Research) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2018/06/21 - 2018/08/02 Research Trainee (Research) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Projects

Grant

Designation Funders
2021/03/01 - 2025/02/28 Biochemical and functional characterization of enzymes from Neisseria gonorrhoeae and Staphylococcus aureus involved in the infection process
UI/BD/151168/2021
10.54499/UI/BD/151168/2021
 Fundação para a Ciência e a Tecnologia
Ongoing
2020/07/27 - 2020/10/28 Aplicação de técnicas bioquímicas ao estudo do citocromo c de Neisseria gonorrhoeae
Verão com Ciência
Research Fellow
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2020/01/01 - 2020/05/31 Detoxification of hydrogen peroxide by Pathogenic bacteria - E. coli tri-haem peroxidase as a model
PTDC/BIA-BQM/29442/2017
Scientific Initiation Fellow
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2021/01/01 - 2025/12/31 Institute for Health and Bioeconomy
LA/P/0140/2020
10.54499/LA/P/0140/2020
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Universidade Nova de Lisboa, Portugal

INESC Microsistemas e Nanotecnologias, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2020/01/01 - 2023/12/31 Applied Molecular Biosciences Unit
UIDB/04378/2020
10.54499/UIDB/04378/2020
Universidade Nova de Lisboa, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2020/01/01 - 2023/12/31 Applied Molecular Biosciences Unit
UIDP/04378/2020
10.54499/UIDP/04378/2020
Universidade Nova de Lisboa, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2020/05 - 2021/02 A novel Fe/S centre biogenesis system dedicated to anaerobic bacteria
Cotutelas Pessoa 2020/2021
Researcher
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
Outputs

Publications

Conference abstract
  1. Barreiro, Daniela S.; Pauleta, Sofia R.; Romão, Margarida. "Electrochemical studies of the interaction between metalloenzymes from Neisseria gonorrhoeae and their physiological partners". Paper presented in XXV Meeting of the Portuguese Electrochemical Society, Coimbra, 2023.
    Published
  2. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Bacterial Nitric Oxide Reductase - Solubilization and Purification of Membrane Proteins". Paper presented in Membrane Protein Expression, Purification and Characterization (mPEPC2), Hamburg, 2022.
  3. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Pauleta, Sofia R.. "Biochemical Characterization of metalloproteins involved in Neisseria gonorrhoeae proliferation in the host". Paper presented in 1st MOSBRI Scientific Conference, Paris, 2022.
  4. Barreiro, Daniela S.; Romão, Margarida; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Assessment of electrontransfer from putative redox complex involved in Neisseria gonorrhoaea anaerobic proliferation at the infection site". Paper presented in NOVAhealth Chronic Disease and Infection: Microbiota and Health., Online, 2021.
  5. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Pauleta, Sofia R.. "Biochemical characterization of Neisseria gonorrhoeae cytochrome c2 and nitrite reductase involved in peroxide detoxification and denitrification". Paper presented in III International Conference NOVAHealth Chronic Disease and Infection, 2020.
    Published
  6. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Gonçalves, Bárbara; Rita G. Sobral; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Copper Proteins form Pathogenic Bacteria - Metal Homeostasis and Denitrification". Paper presented in International Workshop on Chemistry of Group 11 Elements, Caparica, 2020.
  7. Gonçalves, Bárbara; Barreiro, Daniela S.; Rita G. Sobral; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "The role of the multicopper oxidase in copper homeostasis in gram positive pathogens". Paper presented in Congress of Microbiology and Biotechnology, Coimbra, 2019.
  8. Barreiro, Daniela S.; Incorvaia, Simona; Marta S. P. Carepo; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Biochemical characterization of Neisseria gonorrhoeae cytochrome c2 involved in hydrogen peroxide detoxification and denitrification". Paper presented in 24th European Nitrogen Cycle Meeting, Lisboa, 2019.
  9. Gonçalves, Bárbara; Barreiro, Daniela S.; Pauleta, Sofia R.; Rita G. Sobral. "Dealing with copper stress - multicopper oxidases in pathogenic Gram-positive bacteria". Paper presented in XXVI Encontro Nacional da Sociedade Portuguesa de Química, Porto, 2019.
  10. Barreiro, Daniela S.; Favinha, André G.; Martins, Joana N. ; Philip O'Toole; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Hemoglobin-Acrylamide Adduct - A Spectroscopy Study". Paper presented in XXVI Encontro Nacional da Sociedade Portuguesa de Química, Porto, 2019.
Conference poster
  1. Barreiro, Daniela S.; Correia dos Santos, Margarida M.; Carepo, Marta; Pauleta, Sofia R.. "Biochemical characterization of proteins involved in Neisseria gonorrhoeae infection process". Paper presented in 17th European Biological Inorganic Chemistry Conference (EuroBIC-17), 2024.
  2. Barreiro, Daniela S.; Pauleta, Sofia R.; Romão, Margarida. "Electrochemical studies of the interaction between metalloenzymes from Neisseria gonorrhoeae and their physiological partners". Paper presented in XXV Meeting of the Portuguese Electrochemical Society, 2023.
  3. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Bacterial Nitric Oxide Reductase - Solubilization and Purification of an integral inner-membrane metalloprotein". Paper presented in Membrane Protein Expression, Purification and Characterization (mPEPC2), 2022.
  4. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Pauleta, Sofia R.. "Biochemical Characterization of metalloproteins involved in Neisseria gonorrhoeae proliferation in the host". Paper presented in 1st MOSBRI Scientific Conference, 2022.
  5. Barreiro, Daniela S.; Pauleta, Sofia R.; Romão, Margarida. Corresponding author: Romão, Margarida. "Assessment of electrontransfer from putative redox complex involved in Neisseria gonorrhoaea anaerobic proliferation at the infection site". Paper presented in NOVAhealth Chronic Disease and Infection: Microbiota and Health., 2021.
  6. Barreiro, Daniela S.; Correia dos Santos, M. M.; Pauleta, S.; Romão, Margarida. Corresponding author: Romão, Margarida. "Assessment of electron transfer in putative redox complex involved in Neisseria gonorrhoaea anaerobic proliferation at the infection site". Paper presented in IV International Conference NOVAhealth Chronic Disease and Infection, 2021.
  7. Barreiro, Daniela S.; Correia Dos Santos, M.M.; Pauleta, S.. Corresponding author: Pauleta, S.. "Electrochemical study of proteins involved in the infection process of Neisseria gonorrhoeae". Paper presented in XXIV Meeting of the Portuguese Electrochemical Society, 2021.
  8. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Romão, Margarida; Pauleta, Sofia R.. "Biochemical characterization of Neisseria gonorrhoeae proteins involved in anaerobic proliferation". Paper presented in Perspectives in Bioenergetics, 2021.
  9. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Pauleta, Sofia R.. "Biochemical characterization of Neisseria gonorrhoeae cytochrome c2 and nitrite reductase involved in peroxide detoxification and denitrification". Paper presented in III INTERNATIONAL CONFERENCE NOVAHEALTH CHRONIC DISEASE AND INFECTION, 2020.
  10. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Gonçalves, Bárbara; Rita G. Sobral; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Copper Proteins form Pathogenic Bacteria - Metal Homeostasis and Denitrification". Paper presented in International Workshop on Chemistry of Group 11 Elements, 2020.
  11. Gonçalves, Bárbara; Barreiro, Daniela S.; Rita G. Sobral; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "The role of the multicopper oxidase in copper homeostasis in gram positive pathogens". Paper presented in Congress of Microbiology and Biotechnology 2019, 2019.
  12. Barreiro, Daniela S.; Incorvaia, Simona; Marta S. P. Carepo; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Biochemical characterization of Neisseria gonorrhoeae cytochrome c2 involved in hydrogen peroxide detoxification and denitrification". Paper presented in 24th European Nitrogen Cycle Meeting, 2019.
  13. Barreiro, Daniela S.; Favinha, André G.; Martins, Joana N. ; Philip O'Toole; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Hemoglobin-Acrylamide Adduct - a Spectroscopic Study". Paper presented in XXVI Encontro Nacional da Sociedade Portuguesa de Química, 2019.
  14. Barreiro, Daniela S.. "Dealing with copper stress - multicopper oxidases in pathogenic Gram-positive bacteria". Paper presented in XXVI Encontro Nacional da Sociedade Portuguesa de Química, 2019.
  15. Barreiro, Daniela S.. "Dealing with copper in pathogenic Gram-positive bacteria". Paper presented in Gordon Research Conferences in “Cell Biology of Metals”, 2019.
Journal article
  1. Barreiro, Daniela S.; Marta S. P. Carepo; Correia dos Santos, Margarida M.; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Biochemical characterization of cytochrome c2 from Neisseria gonorrhoeae (In preparation)". (2024):
  2. Barreiro, Daniela S.; Oliveira, Ricardo N. S.; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae". Biomolecules 13 8 (2023): https://www.mdpi.com/2218-273X/13/8/1215.
    Open access • Published • 10.3390/biom13081215
  3. Barreiro, Daniela S.; Oliveira, Ricardo N.S.; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Bacterial peroxidases – Multivalent enzymes that enable the use of hydrogen peroxide for microaerobic and anaerobic proliferation". Coordination Chemistry Reviews 485 (2023): 215114. http://dx.doi.org/10.1016/j.ccr.2023.215114.
    Open access • Published • 10.1016/j.ccr.2023.215114
  4. Favinha, André G.; Barreiro, Daniela S.; Martins, Joana N.; O'Toole, Philip; Pauleta, Sofia R.. Corresponding author: Pauleta, Sofia R.. "Acrylamide-hemoglobin adduct: A spectroscopic study". Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 241 (2020): 118644. http://dx.doi.org/10.1016/j.saa.2020.118644.
    Published • 10.1016/j.saa.2020.118644
Preprint
  1. Daniela S. Barreiro; Ricardo N. S. Oliveira; Sofia R. Pauleta. "Biochemical characterization of AniA from Neisseria gonorrhoeae". 2022. https://doi.org/10.1101/2022.04.07.487406.
    10.1101/2022.04.07.487406
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/07/03 Flash-Presentation | Biochemical and Functional Characterization of Metalloenzymes ALS1: Protein Stabilization: Design, Experiments and Assessment (ProteSta)
Institute for Biocomputation and Physics of Complex Systems, BIFI-LACRIMA (Zaragoza, Spain)
2022/09/05 Flash-Presentation | Biochemical and Functional Characterization of Metalloenzymes Practical Course | Membrane Protein Expression, Purification and Characterization (mPEPC2)
EMBL HAMBURG (Hamburg, Germany)
2022/04/20 Electrochemical characterization of Cytochrome c2 from Neisseria gonorrhoeae BioSeminars@UCIBIO
Nova School of Science and Technology (Portugal)
2021/10/20 Electrochemical study of proteins involved in the infection process of Neisseria gonorrhoeae the XXIV Meeting of the Portuguese Electrochemical Society (SPE2021)
Sociedade Portuguesa de Eletroquímica (Portugal)
2021/03/27 Biochemical characterization of Cytochrome c2 - A putative electron shuttle in the Neisseria gonorrohoeae periplasm Digital Colloquium of Chemistry Students in Portugal
ANEQUIMICA
2021/01/21 Cytochrome c2 and Nitrite Reductase from Neisseria gonorrhoeae - A biochemical study BioSeminars@UCIBIO
Nova School of Science and Technology (Caparica, Portugal)
2020/10/21 Aplicação de técnicas bioquímicas ao estudo do citocromo c2 de Neisseria gonorrhoeae Simpósio das Escolas de Verão do LAQV e do UCIBIO
Nova School of Science and Technology (Caparica, Portugal)
2020/02/14 RNS and Peroxide detoxificattion from pathogenic bacteria. What is the role of cytochrome c2? Jornadas Intercalares de Mestrados do DQ e DCV
Nova School of Science and Technology (Caparica, Portugal)
2018/10 Copper tolerance in Enterococcus faecium: unveiling the activity of a multicopper oxidase encoded by cueO gene and its gene transfer to Staphylococcus aureus. Oral Presentation by Sofia R. Pauleta 1st UCIBIO Annual Meeting. Caparica, Portugal
(Caparica, Portugal)

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2024/04/10 - 2024/04/10 EXPO FCT is the open day of FCT - NOVA that allows high-school students to learn more about Science and Engineering and the different Departments and Research Centers of the university.
Other
 EXPO FCT 2024
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2023/07/03 - 2023/07/07 The course covers different biophysical techniques such as fluorescence spectroscopy, circular dichroism, and differential scanning calorimetry, as well as different approaches for protein engineering directed at improving stability. The sessions are complemented with practical tutorials on data analysis and computational calculations, followed by critical discussion of the results. Participants have the opportunity to describe their own projects related to protein stability.
Workshop
 ALS1: Protein Stabilization: Design, Experiments and Assessment (ProteSta)
Institute for Biocomputation and Physics of Complex Systems, BIFI-LACRIMA, Spain
2023/04/20 - 2023/04/20 EXPO FCT is the open day of FCT - NOVA that allows high-school students to learn more about Science and Engineering and the different Departments and Research Centers of the university.
Other
 EXPO FCT 2023
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/10/10 - 2022/10/10 This event, which celebrates scientific culture in Portugal and around the world, is aimed at all science enthusiasts and those curious about science, of all ages, and promises to continue to make history and show that science, in addition to being fun, is part of our day. -a-day.
Other
 FIC.A – Festival internacional de Ciência
Camara Municipal Oeiras, Portugal
2022/09/24 - 2022/10/01 Training School aimed at PhD Students and Postdocs. Focused on the application of microbes in sustainable wastewater treatment and valuable products recovery from waste. Included 14 lectures by senior members of top European Universities and 2 technical visits to Pakmaya (large scale yeast fermentation industry) and Bursa Wastewater Treatment plant.
Workshop
 Application of microbial processes in sustainable production, and the impact of low pH on these
Marmara Üniversitesi Mühendislik Fakültesi, Turkey

EuroMicropH - COST Action CA18113, Belgium
2022/09/04 - 2022/09/12 This course trains PhD students and early career postdocs with the latest developments in membrane protein production, characterization and structure determination methods. Modern protein expression techniques in various hosts will be presented and students will get acquainted with new crystallization, Cryo-EM and reconstitution techniques. The presence of a crystallization and cryo-EM facility as well as beamlines for SAXS and macromolecular crystallography at the venue will be employed to perform integrated structural biology experiments.
Workshop
 Practical Course | Membrane Protein Expression, Purification and Characterization (mPEPC2)
EMBL Hamburg, Germany
2022/04/27 - 2022/04/27 EXPO FCT is the open day of FCT - NOVA that allows high-school students to learn more about Science and Engineering and the different Departments and Research Centers of the university.
Other
 EXPO FCT 2022
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021/10/22 - 2021/10/29 The School covers methodological and applied aspects of NMR, EPR and bioinformatics, with a specific focus on metalloproteins
Seminar
 Training School | Fundamentals of magnetic resonance spectroscopies and metal trafficking
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine, Italy

Technische Universität Berlin, Germany