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Eliana Fernandes graduated as a MSc in Chemical Engineering at the Faculty of Science and Technology from the University of Coimbra in 2019. Her thesis, entitled "Preparation of silica aerogel composites with recycled rubber for thermal insulation of buildings," was developed under the project Tyre 4 Build Ins and aimed to use recycled tyre rubber resin-bonded for building insulation systems towards energy efficiency. This thesis received the classification of 18/20 for the high-quality work and dedication. Since September 2019 she has participated as a research fellow in the FCT project "Monitoring neurometabolic markers in vivo using microelectrode-based biosensors" - (POCI-01-0145-FEDER-028261) at the Faculty of Pharmacy, University of Coimbra (UC) and in the group Redox Biology and Brain Sensing fom Center of Neuroscience and Cell Biology (CNC-UC). In her participation in this project, she learned and improved her skills regarding the fabrication of microelectrodes and how to perform their electrochemical characterization, evaluation, and optimization. She also gained expertise in performing electrochemical measurements (cyclic voltammetry, amperometry, impedance spectrum spectroscopy) and data analysis (Origin, GraphPad, Matlab). In the framework of this project, she has published 4 full research articles in peer-reviewed journals, including 1 as the first author (Molecules, IF. 4.41, Q2 journal in Chemistry, Multidisciplinary), and 3 as co-author in Q1 journals in areas of Chemistry (Analytical), Electrochemistry and Biochemistry & Molecular Biology (Redox Biology, IF. 11.80, Biosensors and Bioelectronics, IF. 10.61 and Sensors and Actuators B: Chemical IF. 7.34). She also presented posters in 1 national (Analítica2020) and 1 international (72nd Annual Meeting of the International Society of Electrochemistry) conference. During this period, she successfully completed the theoretical and practical course on Laboratory Animal Sciences designed according to the FELASA recommendations for Category B (carrying out procedures on animals). Notably, she has a Cambridge Certificate in Advanced English (Level C1) that evidences her skills in the English language, and she actively participated in workshops and seminars that improved her knowledge in various areas and that empowered her with the tools needed to excel in her future academic and professional career. During her assignments, she actively contributed to the training of young professionals’ career, as shown by the mentoring/tutoring of 2 MSc thesis, and 8 BSc and MSc students through their extracurricular internships. In September 2021, she enrolled the Ph.D. programme in Pharmaceutical Sciences, Faculty of Pharmacy, University of Coimbra.
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Identification

Personal identification

Full name
Eliana Maria Simões Fernandes

Citation names

  • Fernandes, Eliana

Author identifiers

Ciência ID
5C15-C846-C9B7
ORCID iD
0000-0001-8123-7007

Email addresses

  • eliana.fernandes@ff.uc.pt (Professional)

Knowledge fields

  • Engineering and Technology - Chemical Engineering - Chemical Engineering
  • Exact Sciences - Chemical Sciences - Electrochemistry
  • Exact Sciences - Chemical Sciences - Analytical Chemistry

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2021 - 2025
Ongoing
 Pharmaceutical Sciences (Doutoramento)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2021/11/15 - 2022/02/24
Concluded
 Modular Training in Laboratory Animal Science (Outros)
Center for Neuroscience and Cell Biology (CNC), Portugal

Coimbra Institute for Clinical and Biomedical Research, Portugal
2022/01/31 - 2022/02/04
Concluded
 Advanced Imaging (Outros)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2021/11/01 - 2022/01/25
Concluded
 Theoretical Course on Laboratory Animal Sciences (FELASA - Category B) (Outros)
Sociedade Portuguesa de Ciências em Animais de Laboratório, Portugal
2021/07/13 - 2021/07/15
Concluded
 Bioanalytical Methods for Neurochemical Research (Outros)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2019
Concluded
 Chemical Engineering (Mestrado)
Major in Processo, Ambiente e Energia
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Preparation of silica aerogel composites with recycled rubber for thermal insulation of buildings" (THESIS/DISSERTATION)
2016
Concluded
 Chemical Engineering (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2013
Concluded
 Cambridge Advanced English (Outros)
International House Viseu, Portugal
 C1
Affiliation

Science

Category
Host institution 		Employer
2019/09 - 2022 Researcher (Research) Universidade de Coimbra Faculdade de Farmácia, Portugal
Universidade de Coimbra Faculdade de Farmácia, Portugal
(...)
Projects

Grant

Designation Funders
2019/09 - 2022 Monitoring neurometabolic markers in vivo using microelectrode-based biosensors
POCI-01-0145-FEDER-028261
Research Fellow
Universidade de Coimbra Faculdade de Farmácia, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Ongoing
Outputs

Publications

Conference poster
  1. Fernandes, Eliana; Ledo, Ana; Barbosa, Rui M.. "Amperometric Biosensors For High Spatial And Temporal Resolution Monitoring Of Lactate In Rat Brain". Paper presented in Pharma PhD Day, 2023.
  2. Fernandes, Eliana; Ledo, Ana; Barbosa, Rui M.. "Amperometric biosensors for high spatial and temporal resolution monitoring of lactate in vivo in rat brain". Paper presented in X Congresso Iberoamericano de Ciências Farmacêuticas, 2023.
  3. Fernandes, Eliana; Barbosa, Rui M.; Ledo, Ana. "Amperometric measurements of lactate and oxygen concentration dynamics in vivo in the rat brain". Paper presented in XXV Meeting of the Portuguese Electrochemical Society, 2023.
  4. Fernandes, Eliana; Barbosa, Rui M.; Ledo, Ana. "Development of Microelectrode Based Biosensors for Monitoring Lactate and Oxygen Dynamics in Vivo in the Rat Brain". Paper presented in 1st FEBS Redox Medicine Workshop: from cellular signaling to systems physiology and therapeutic targets, 2023.
  5. Fernandes, Eliana; Dias, Cândida; Regiart, Matias; Vicente, André T.; Ledo, Ana; Barbosa, Rui M.. Corresponding author: Barbosa, Rui M.. "Microelectrode Biosensors for Real-Time Monitoring Lactate Trafficking in the Extracellular Space of Brain Tissue". Paper presented in 72nd Annual Meeting of the International Society of Electrochemistry, 2021.
  6. Fernandes, Eliana; Ledo, Ana; Barbosa, Rui M.. "Development of a microelectrode array biosensor for monitoring lactate dynamics in the brain". Paper presented in Analítica2020, 2020.
Journal article
  1. Fernandes, Eliana; Ledo, Ana; Gerhardt, Greg A.; Barbosa, Rui M.. "Amperometric bio-sensing of lactate and oxygen concurrently with local field potentials during status epilepticus". Talanta 268 (2023): 125302. http://dx.doi.org/10.1016/j.talanta.2023.125302.
    Published • 10.1016/j.talanta.2023.125302
  2. Dias, Cândida; Fernandes, Eliana; Barbosa, Rui M.; Laranjinha, João; Ledo, Ana. Corresponding author: Ledo, Ana. "Astrocytic Aerobic Glycolysis Provides Lactate to Support Neuronal Oxidative Metabolism in the Hippocampus". Biofactors (2023):
    In press
  3. Dias, Cândida; Fernandes, Eliana; Barbosa, Rui M.; Ledo, Ana. "A Platinized Carbon Fiber Microelectrode-Based Oxidase Biosensor for Amperometric Monitoring of Lactate in Brain Slices". Sensors 22 18 (2022): 7011. http://dx.doi.org/10.3390/s22187011.
    10.3390/s22187011
  4. Ledo, Ana; Fernandes, Eliana; Salvador, Armindo; Laranjinha, João; Barbosa, Rui M.. "In vivo hydrogen peroxide diffusivity in brain tissue supports volume signaling activity". Redox Biology 50 (2022): 102250-102250. https://doi.org/10.1016/j.redox.2022.102250.
    10.1016/j.redox.2022.102250
  5. Regiart, Matias; Ledo, Ana; Fernandes, Eliana; Messina, German A.; Brett, Christopher M.A.; Bertotti, Mauro; Barbosa, Rui M.. "Highly sensitive and selective nanostructured microbiosensors for glucose and lactate simultaneous measurements in blood serum and in vivo in brain tissue". Biosensors and Bioelectronics (2022): https://doi.org/10.1016/j.bios.2021.113874.
    10.1016/j.bios.2021.113874
  6. Fernandes, Eliana; Ledo, Ana; Barbosa, Rui M.. "Design and Evaluation of a Lactate Microbiosensor: Toward Multianalyte Monitoring of Neurometabolic Markers In Vivo in the Brain". Molecules (2022): https://www.mdpi.com/1420-3049/27/2/514.
    10.3390/molecules27020514
  7. Dias, Cândida; Fernandes, Eliana; Barbosa, Rui M.; Ledo, Ana. Corresponding author: Ledo, Ana. "Monitoring Extracellular Lactate in Hippocampal Brain Slices with Oxidase-Based Microbiosensors: The Effect of Glutamate". Analytical Biochemistry (2022):
    Under revision
  8. Ledo, Ana; Fernandes, Eliana; Laranjinha, João; Barbosa, Rui M.. "Diffusion and Removal Kinetics of Hydrogen Peroxide in Brain Tissue". Free Radical Biology and Medicine 165 (2021): 28. http://dx.doi.org/10.1016/j.freeradbiomed.2020.12.326.
    Published • 10.1016/j.freeradbiomed.2020.12.326
  9. Ledo, Ana; Fernandes, Eliana; Quintero, Jorge E.; Gerhardt, Greg A.; Barbosa, Rui M.. "Electrochemical Evaluation of a Multi-Site Clinical Depth Recording Electrode for Monitoring Cerebral Tissue Oxygen". Micromachines 11 7 (2020): 632. http://dx.doi.org/10.3390/mi11070632.
    10.3390/mi11070632
  10. Ledo, Ana; Fernandes, Eliana; Brett, Christopher M.A.; Barbosa, Rui M.. "Enhanced selectivity and stability of ruthenium purple-modified carbon fiber microelectrodes for detection of hydrogen peroxide in brain tissue". Sensors and Actuators B: Chemical (2020): 127899-127899. https://doi.org/10.1016/j.snb.2020.127899.
    10.1016/j.snb.2020.127899
Thesis / Dissertation
  1. "Preparation of silica aerogel composites with recycled rubber for thermal insulation of buildings". Master, Universidade de Coimbra Faculdade de Ciencias e Tecnologia, 2019.
Activities

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2022/03/25 - 2022/03/25 Elaboração de Planos de Gestão de Dados - Ferramenta Argos e Modelo FCT
Workshop
Instituto de Investigação Interdisciplinar da Universidade de Coimbra, Portugal
2021/10/04 - 2021/11/04 Curso Green Belt Six Sigma
Seminar
2021/09/13 - 2021/11/04 Curso International Valorización de Residuos, Bioeconomía y Economía Circular
Seminar
2021/09/22 - 2021/09/22 Salinity Sensor with a Sensit Smart by PalmSens
Workshop
2021/03/17 - 2021/03/17 Webinar about electrochemical, enzymatic biosensors - PalmSens
Seminar
2021/03/12 - 2021/03/12 Mendeley: gestor automático de referências bibliográficas
Workshop
Universidade de Coimbra Biblioteca das Ciências da Saúde, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2022/03 - 2024/08 Tecnologias Analíticas Ciências Bioanalíticas (Licenciatura) Universidade de Coimbra Faculdade de Farmácia, Portugal
2022/03 - 2024/08 Análise Instrumental Ciências Bioanalíticas (Licenciatura) Universidade de Coimbra Faculdade de Farmácia, Portugal

Mentoring / Tutoring

Topic Student name
2021/11/01 - Current NEF/AAC Extracurricular Internships - Evalutation and optimization of the platinization process in carbon fiber based microelectrodes Alexandre Correia
2021/09/15 - Current MSc Thesis Project - Development and analytical validation of biosensors for measurement of redox and/or metabolic markers in clinical samples Raquel Sofia Moita
2020/09/15 - Current MSc Thesis Project - Monitoring Neurometabolic Markers in Epileptic States and Trauma João Miguel Vieira
2021/09/01 - 2021/09/30 Programa Verão com Ciência - Electrochemical evaluation of Clinical Depth Recording Electrode Ana Matos
2020/09/15 - 2021/06/30 NEF/AAC Extracurricular Internship - Screen-printed microlectrodes (SPEs) electrochemical evaluation and enzyme immobilization Ana Sousa, Margarida Ribeiro
2020/09/15 - 2021/06/30 NEF/AAC Extracurricular Internship - Carbon fiber based microelectrode (30 vs. 7 micrometers in diameter) electrochemical evaluation and enzyme immobilization André Vicente
2019/09/15 - 2020/06/30 NEF/AAC Extracurricular Internship - Development and study of electrochemical performance of carbon fiber based microelectrodes André Vicente, Marina Reva, Mariana Marques
Distinctions

Award

2023 Melhor Poster - Bioquímica, Análises Clínicas, Forenses e Toxicológicas
Comissão Científica do X Congresso Iberoamericano de Ciências Farmacêuticas, Portugal