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Ana Filipa Sequeira. Completed the Doutoramento in Doutoramento em Ciências Biológicas na especialidade de Ciências Biológicas e Biomédicas in 2016 by Universidade de Lisboa Faculdade de Medicina Veterinária, Mestrado in Mestrado em Biotecnologia in 2010 by Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia and Licenciatura in Química Aplicada in 2007 by Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia. Is Senior Research Scientist in NZYTech Lda. Published 12 articles in journals. Has 2 section(s) of books. Works in the area(s) of Natural sciences with emphasis on Biological Sciences with emphasis on Molecular Biology. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: Gene Synthesis; Cloning; Recombinant Protein Expression; qPCR; PCR; DNA recombinant technology; Molecular Diagnostics; Sequencing; High-throughput Gene Synthesis; High-throughput Cloning; High-throughput Protein Expression; Recombinant Protein Purification; .
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Identification

Personal identification

Full name
Ana Filipa Sequeira

Citation names

  • Sequeira, Ana
  • Sequeira, AF

Author identifiers

Ciência ID
5A19-D120-708D
ORCID iD
0000-0001-8445-6221

Email addresses

  • filipa.sequeira@nzytech.com (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2016
Concluded
 Doutoramento em Ciências Biológicas na especialidade de Ciências Biológicas e Biomédicas (Doutoramento)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
"DEVELOPMENT OF A NOVEL PLATFORM FOR HIGH-THROUGHPUT GENE DESIGN AND ARTIFICIAL GENE SYNTHESIS TO PRODUCE LARGE LIBRARIES OF RECOMBINANT VENOM PEPTIDES FOR DRUG DISCOVERY" (THESIS/DISSERTATION)
 Distinção e louvor
2010
Concluded
 Mestrado em Biotecnologia (Mestrado)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"O envolvimento da via de sinalização FGF/FGFR na etiologia do autismo: análise genética" (THESIS/DISSERTATION)
 18
2007
Concluded
 Química Aplicada (Licenciatura)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Fenilalanina Hidroxilase Humana Recombinante: caracterização de formas quiméricas e identificação de formas hibridas" (THESIS/DISSERTATION)
 14
Affiliation

Others

Category
Host institution 		Employer
2021/10/01 - Current R&D Director NZYTech Lda, Portugal
2019/01/01 - 2021/09/30 Senior Research Scientist NZYTech Lda, Portugal
NZYTech Lda, Portugal
2016/09/30 - 2018/12 Research Scientist NZYTech Lda, Portugal
2018 - 2018 Research Scientist Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal
2015 - 2015 Research Scientist Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal
2011 - 2013 Research Scientist Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal
2011/03/01 - 2012/02/28 Técnico Superior de projecto de investigação. Investigação e desenvolvimento de produtos de Biologia Molecular NZYTech Lda, Portugal
2007/09/01 - 2011/02/20 Research Technician - Autism human genetic Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2007 - 2009 Research Technician - Autism human genetic Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2007/03 - 2007/11 Estagiário de licenciatura Universidade de Lisboa Faculdade de Farmácia, Portugal
Projects

Grant

Designation Funders
2011/11/01 - Current VENOMICS: High-throughput peptidomics and transcriptomics of animal venoms for discovery of novel therapeutic peptides and innovative drug development
278346
Researcher
NZYTech Lda, Portugal
 European Commission
Concluded
2021/07/01 - 2023/06/30 Glycomed - A new generation of enzymes for biofilm dispersion and immunomodulation in cancer. LISBOA-01-0247-FEDER-047033
LISBOA-01-0247-FEDER-047033
Post-doc
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal
Concluded
2019/01/01 - 2020/12/31 SuperPol: Desenvolvimento de uma nova geração de DNA polimerases recombinantes de alta eficácia com origem em organismos marinhos extremófilos. SuperPol LISBOA-01-0247- FEDER-038447
SuperPol LISBOA-01-0247- FEDER-038447
Post-doc
NZYTech Lda, Portugal
Concluded
2007/05/01 - 2010/05/31 Autism Genome Project (AGP) Consortium - Whole Genome Association and Copy Number Variation Study
7568
Research Fellow
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

Nationwide Children's Hospital, United States
Concluded
Outputs

Publications

Book chapter
  1. Sequeira, Ana. "High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay". 2019.
    10.1007/978-1-4939-9624-7_21
  2. Sequeira, Ana. "High-Throughput Production of Oxidized Animal Toxins in Escherichia coli". 2019.
    10.1007/978-1-4939-9624-7_7
Journal article
  1. Turchetto J; Sequeira AF; Ramond L; Peysson F; Brás JL; Saez NJ; Duhoo Y; et al. "High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery.". (2017): http://europepmc.org/abstract/med/28095880.
    10.1186/s12934-016-0617-1
  2. Sequeira AF; Turchetto J; Saez NJ; Peysson F; Ramond L; Duhoo Y; Blémont M; et al. "Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli.". (2017): http://europepmc.org/abstract/med/28093085.
    10.1186/s12934-016-0618-0
  3. Sequeira AF; Brás JLA; Fernandes VO; Guerreiro CIPD; Vincentelli R; Fontes CMGA. "A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli.". Methods in molecular biology (Clifton, N.J.) (2017): http://europepmc.org/abstract/med/28540703.
    10.1007/978-1-4939-7060-5_7
  4. Sequeira AF; Brás JL; Guerreiro CI; Vincentelli R; Fontes CM. "Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins.". (2016): http://europepmc.org/abstract/med/27905914.
    10.1186/s12896-016-0316-3
  5. Sequeira, Ana Filipa; Brás, Joana L. A.; Guerreiro, Catarina I. P. D.; Vincentelli, Renaud; Fontes, Carlos M. G. A.. "Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins". BMC Biotechnology 16 1 (2016): http://dx.doi.org/10.1186/s12896-016-0316-3.
    10.1186/s12896-016-0316-3
  6. Sequeira AF; Guerreiro CI; Vincentelli R; Fontes CM. "T7 Endonuclease I Mediates Error Correction in Artificial Gene Synthesis.". (2016): http://europepmc.org/abstract/med/27334914.
    10.1007/s12033-016-9957-7
  7. Correia CT; Conceição IC; Oliveira B; Coelho J; Sousa I; Sequeira AF; Almeida J; et al. "Recurrent duplications of the annexin A1 gene (ANXA1) in autism spectrum disorders.". (2014): http://europepmc.org/abstract/med/24720851.
    10.1186/2040-2392-5-28
  8. Gilling M; Rasmussen HB; Calloe K; Sequeira AF; Baretto M; Oliveira G; Almeida J; et al. "Dysfunction of the Heteromeric KV7.3/KV7.5 Potassium Channel is Associated with Autism Spectrum Disorders.". (2013): http://europepmc.org/abstract/med/23596459.
    10.3389/fgene.2013.00054
  9. Casey JP; Magalhaes T; Conroy JM; Regan R; Shah N; Anney R; Shields DC; et al. "A novel approach of homozygous haplotype sharing identifies candidate genes in autism spectrum disorder.". Human genetics (2012): http://europepmc.org/abstract/med/21996756.
    10.1007/s00439-011-1094-6
  10. Leblond CS; Heinrich J; Delorme R; Proepper C; Betancur C; Huguet G; Konyukh M; et al. "Genetic and functional analyses of SHANK2 mutations suggest a multiple hit model of autism spectrum disorders.". (2012): http://europepmc.org/abstract/med/22346768.
    10.1371/journal.pgen.1002521
  11. Correia CT; Almeida JP; Santos PE; Sequeira AF; Marques CE; Miguel TS; Abreu RL; Oliveira GG; Vicente AM. "Pharmacogenetics of risperidone therapy in autism: association analysis of eight candidate genes with drug efficacy and adverse drug reactions.". (2010): http://europepmc.org/abstract/med/19997080.
    10.1038/tpj.2009.63
  12. Correia CT; Coutinho AM; Sequeira AF; Sousa IG; Lourenço Venda L; Almeida JP; Abreu RL; et al. "Increased BDNF levels and NTRK2 gene association suggest a disruption of BDNF/TrkB signaling in autism.". (2010): http://europepmc.org/abstract/med/20662941.
    10.1111/j.1601-183X.2010.00627.x
  13. Pinto D; Pagnamenta AT; Klei L; Anney R; Merico D; Regan R; Conroy J; et al. "Functional impact of global rare copy number variation in autism spectrum disorders.". (2010): http://europepmc.org/abstract/med/20531469.
    10.1038/nature09146