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I graduated in Chemical Engineering/Biotechnology (15/20) from Instituto Superior Técnico, UTL in 2001 and did my PhD in Biotechnology in 2006 at the same institution. My research, under Prof. Joaquim Cabral’s supervision and Prof. Karl-Erich Jaeger from the Institut für Molecular Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf focused on in vitro evolution of lipolytic enzymes, emphasizing on improving their stability in the presence of surfactants. This led to 5 publications (2 as first and correspondent author and a journal cover). In 2006, I joined the Microbial and Enzyme Technology group at ITQB NOVA to pursue postdoctoral studies mentored by Prof. Lígia O. Martins and later an Auxiliary Researcher. My research focuses on the evolution and biochemical characterization of oxidoreductive bacterial enzymes (multicopper oxidases, azo/quinone reductases, and DyP-type peroxidases), aiming to enhance their biotechnological applications and gain mechanistic insights. Since 2018, I am a Researcher in Prof. Lígia O. Martins’ group at ITQB NOVA where I have honed my skills in various aspects of applied biotechnology, particularly in protein engineering and evolution, a cutting-edge area where I specialize in applying directed evolution methodologies and have mastered molecular biology, biochemistry, and microbiology techniques. Overall, I have 34 publications: 27 papers in peer-reviewed international journals, two as corresponding author (times cited:+1000) and 7 peer-reviewed conference proceedings, 1 book chapter, 27 oral and 52 poster communications at national and international conferences. I was the PI of an exploratory project and the Co-PI of a national project and collaborated on 3 national and 2 EU projects. Currently, I am Co-PI of the national project “Broadening the spectrum of biocatalysts, bioprocesses, and bio-products in industrial biotechnology (Biocat4Suschem)” and team member of COZYME (COST-Action; https://cozyme.eu/) and McGEA (MSCA-Staff Exchange). I am/was involved in protein engineering approaches and enzyme production, purification, and characterization. I have co-supervised 6 M.Sc., 5 graduate, 9 undergraduate, 2 Erasmus, 5 summer internships, 8 lab rotations and 6 high-school students. Additionally, I mentored younger team members, contributing to both the team's success and the professional development of my colleagues. From 2017 to 2021, I took the role of instructor, delivering both theoretical and hands-on sessions for the Biocatalysis unit in two programs: the Master's degree in Biotechnology for Sustainability and the PhD in Sustainable Chemistry. Simultaneously, I held the position of Invited Assistant Professor at the Escola Superior Tecnológica do Barreiro of Instituto Politécnico de Setúbal, contributing to the Master's program in Chemical and Biological Engineering.
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Identification

Personal identification

Full name
Vânia Sofia Soares Pereira Brissos

Citation names

  • Brissos, Vânia

Author identifiers

Ciência ID
5114-E972-5B61
ORCID iD
0000-0002-8171-0113
Researcher Id
M-5789-2013
Scopus Author Id
24079497500

Email addresses

  • vbrissos@itqb.unl.pt (Professional)

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology - Biocatalysis
  • Natural sciences - Biological Sciences - Biochemistry
  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
French Beginner (A1)
German Beginner (A1) Beginner (A1)
Portuguese (Mother tongue)
Education
Degree Classification
2002/09/01 - 2006/11/26
Concluded
 Biotecnologia (Doutoramento)
Major in Sem especialidade
Universidade de Lisboa Instituto Superior Técnico, Portugal
"In Vitro Evolution of Lipolytic Enzymes Stabilization of Cutinase Against Surfactants" (THESIS/DISSERTATION)
1996/09 - 2001/12
Concluded
 Engenharia Química (Licenciatura)
Major in Biotecnologia
Universidade de Lisboa Instituto Superior Técnico, Portugal
"n/a" (THESIS/DISSERTATION)
 15
Affiliation

Science

Category
Host institution 		Employer
2025/02/01 - Current Invited Auxiliary Researcher (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018/11/01 - 2024/10/31 Researcher (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2006/12/01 - 2018/10/31 Postdoc (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2002/10/01 - 2006/11/30 Researcher (Research) Universidade de Lisboa Instituto Superior Técnico, Portugal
2003/04/01 - 2004/12/31 Visiting Researcher (Research) Heinrich-Heine-Universität Düsseldorf Institut für Molekulare Enzymtechnologie, Germany
2002/03/01 - 2002/09/30 Researcher (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2017/11 - 2021/03 Invited Adjunct Teacher (Polytechnic Teacher) Instituto Politécnico de Setúbal, Portugal
Projects

Grant

Designation Funders
2024 - 2028 McGEA: Metalloenzymes and Cells for Green Environmental Alternatives
GE 101183014
Team member
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
Ongoing
2023 - 2026 Broadening the spectrum of biocatalysts, bioprocesses, and bio-products in industrial biotechnology (Biocat4Suschem)
C492089301-00086080
Co-Principal Investigator (Co-PI)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2022 - 2026 COZYME: Establishing a Pan-European Network on Computational Redesign of Enzymes
CA21162
Team member
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 European Commission
Ongoing
2019 - 2024 B-LigZymes: Bacterial Enzymes and Bioprocesses for Lignin Valorisation
824017
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 European Commission
Concluded
2019/05/01 - 2023/10/31 Selective Modifications of ARomatics through Biocatalytic Oxidations
info:eu-repo/grantAgreement/EC/H2020/837890/EU
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 European Commission
Concluded
2019 - 2022 Iterative Laboratory-Computational Evolution of Bacterial Ligninolytic Enzymes
PTDC/BII-BBF/29564/2017
Co-Principal Investigator (Co-PI)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019 - 2022 Oxidoreductase-based electrochemical biosensors and bioreactors: rational design guided by biophysical characterization
PTDC/BIA-BFS/31026/2017
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2022 LignoCOST Establishing a Pan-European Network on the Sustainable Valorisation of Lignin.
CA17128
Team member
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 European Commission
Concluded
2016 - 2019 Biocatalysis for tackling lignin recalcitrance
PTDC/BBB-EBB/0122/2014
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2014 - 2018 LIGNOVAL Valorisation of lignocelluloses biomass side streams for sustainable production of chemicals, materials & fuels using low environmental impact technologies.
FP13066
Team member
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 European Commission
2014/02/01 - 2015 Discovery and characterization of ligninocellulolytic bacterial enzymes
info:eu-repo/grantAgreement/FCT/3599-PPCDT/134957/PT
Principal investigator
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2007/01/01 - 2008 Nano-Engineering of Bacterial Laccases
info:eu-repo/grantAgreement/FCT/3599-PPCDT/82550/PT
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2006/12/01 - 2007/12/31 White Biotechnology for Added value Products from Renewable Plant Polymers: Design of tailor-made Biocatalysts and New Industrial Processes
FP6-2004-NMP-NI-4/026456
Post-doc Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 European Commission
Concluded
Outputs

Publications

Book chapter
  1. Brissos, Vânia; Durão, Paulo; Carolina Ferro Rodrigues; Pinho e Melo, Eduardo; Martins, Lígia O.. "Optimized protocols to measure the stability of ligninolytic enzymes". In Lignin-Degrading Enzymes, edited by Tim Bugg; Juan Carro Aramburu. 2025.
    In press
Conference paper
  1. Brissos, V.; Santos, C.; Santos, J.M.; Guerreiro, M.P.. "The VASelfCare T2D project plan: Fostering innovation through the StartUp Research program". Paper presented in HCIST, Vilamoura, 2021.
    10.1016/j.procs.2021.01.242
  2. Brissos, Vânia; Tavares, Diogo; Duarte, Zuleica; Martins, Lígia. Corresponding author: Martins, Lígia. "Engineering bacterial ligninolytic enzymes for lignin recalcitrance". Paper presented in 15th European Workshop on Lignocellulosics and Pulp (EWLP2018), Aveiro, 2018.
  3. Santos, Diana; Mendes, Sónia; Brissos, Vânia; van Berkel, W.J.H. ; Martins, Lígia. Corresponding author: Martins, Lígia. "Improving a bacterial pyranose 2-oxidase using a combination of rational and directed evolution for biosensor application". Paper presented in 18th European Congress of Biotechnology (ECB2018), Geneva, 2018.
  4. Brissos, Vânia; Gonçaves, Nádia; Gonçalvez, AM. ; Melo, EP; Martins, Lígia. "Stabilization of FMN-dependent NADPH:dye/quinone reductase from Pseudomonas putida by directed evolution". Paper presented in 9th European Biophysics Congress (EBSA 2013), lisbon, 2013.
  5. Mendes, Sónia; Sezer, M. ; Santos, Ana; Todorovic, Smilja; Martins, Lígia. "Catalytic and spectroscopic characterization of two bacterial dye-decolourising peroxidases". Paper presented in 9th European Biophysics Congress (EBSA 2013), Lisbon, 2013.
  6. P C de Barros, Dragana; Brissos, Vânia; Fonseca, L.P. ; Cabral, Joaquim. "Integrated biocatalyst design – Cutinase, a case study". Paper presented in 13th European Congress on Biotechnology (ECB13), 2007.
Conference poster
  1. Catarina Coelho; Taborda, André; Diogo Silva; Verissimo, A; Brissos, Vânia; Igor Tiago; Martins, Lígia O.. "Characterization of a new lactate dehydrogenase from Porcellio dilatatus gut microbiome". Paper presented in Novel Enzymes, 2025.
  2. Catarina Coelho.; Taborda, André; Diogo Silva.; Borges, Patricia T.; Brissos, Vânia; Igor Tiago; Martins, Lígia. "NEW NAD-INDEPENDENT D-LACTATE DEHYDROGENASE FROM GUT MICROBIOME, THROUGH SHOTGUN METAGENOMIC MINING". Paper presented in 11th Edition of OxiZymes meeting - Lublin, 2024.
  3. Lança Martins, Maria; Hernandez, Guillem; Brissos, Vânia; Martins, Lígia O.; Cordeiro, TN. Corresponding author: Cordeiro, TN. "A Hybrid-NMR Approach to Study Substrate Binding in directed evolution". Paper presented in Instruct Biennial Structural Biology, Cascais, Lisbon Portugal, 23 – 24 May 2024, 2024.
  4. Brissos, Vânia; Borges, Patricia T.; Estevinho, Ricardo; Maria robalo; Martins, Lígia O.. "Alkali-and Hyperthermo-stable Engineered Laccase for Lignin-related Phenolics". Paper presented in 16th International Symposium on Biocatalysis and Biotransformations (Biotrans 2023), La Rochelle, France., 2023.
  5. Borges, Patricia T.; Brissos, Vânia; Carlos Frazao; Martins, Lígia. "Role of a flexible loop in the substrate selectivity of an engineered variant of hyperthermophilic multicopper oxidase McoP". Paper presented in 16th International Symposium on Biocatalysis and Biotransformations (Biotrans 2023), La Rochelle, France, 2023.
  6. Barbosa, Catarina; Silveira, Célia; Silva, Diogo; Brissos, Vânia; Martins, Lígia O.; Todorovic, Smilja. "Closer to a Functional H2O2 Biosensor Using Immobilized Dye-decolorizing Peroxidases". Paper presented in 10th OxiZymes, 2022.
  7. Rodrigues, Carolina; Borges, Patricia T.; Scocozza, Magali F.; Silva, Diogo; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia. "Unraveling the role of conserved loops around the heme pocket in the function and stability of BsDyP". Paper presented in 10th Oxizymes Meeting, 2022.
  8. Martins, Maria; Hernandez, Guillem; Brissos, Vânia; Martins, Lígia; Cordeiro, TN. "NMR Studies of Transient Enzyme-Substrate Interactions". Paper presented in TIMB3 Final Meeting, 2022.
  9. Rodrigues, Carolina; Borges, Patricia T.; Brissos, Vânia; Taborda, André; Martins, Lígia O.. "Biochemical, kinetic, and structural analysis of a BsDyP hit variant with improved activity for phenolic compounds". Paper presented in 3rd Aachen Protein Engineering Symposium (AcES), 2021.
  10. Rodrigues, Carolina; Taborda, André; Brissos, Vânia; Martins, Lígia. Corresponding author: Martins, Lígia. "Improving the activity of BsDyP for phenolic compounds using a laboratory enzyme evolution approach". Paper presented in European Federation of Biotechnology Virtual Conference 2021, 2021.
  11. Rodrigues, Carolina; Brissos, Vânia; Taborda, André; Martins, Lígia O.. "Improving the activity of BsDyP for phenolic compounds using a laboratory evolution approach". Paper presented in EFB2021, 2021.
  12. De Simone, Mario; Alvigini, Laura; Ruivo, Margarida; Brissos, Vânia; Pinho e Melo, Eduardo; Mattevi, Andrea; Martins, Lígia O.. "Variations close to the non-heme iron centre: structural, biochemical, kinetic and stability studies of mutant S283F of NOV1 oxygenase". Paper presented in EFB2021 - Virtual Conference, 2021.
  13. Ruivo, Margarida; De Simone, Mario; Brissos, Vânia; Martins, Lígia O.. "Optimization of medium-throughput approaches for construction of mutant libraries and activity/stability screening". Paper presented in Biocatalysis Open Day 2020, 2020.
  14. Rodrigues, Carolina; Brissos, Vânia; Martins, Lígia O.. "Tailor BsDyP for biorefinery applications using a laboratory enzyme evolution approach". Paper presented in Biocatalysis Open Day 2020, 2020.
  15. Rodrigues, Carolina; Brissos, Vânia; Martins, Lígia O.. "New enzymes for biorefinery applications: towards the production of bio-resins". Paper presented in Microbiotec ¿19 Congress, 2019.
  16. Barbosa, Catarina; Silveira, Célia M.; Brissos, Vânia; Martins, Lígia O.; Todorovic, Smilja. "Development of highly efficient hydrogen peroxide biosensors". Paper presented in Twin to Illuminate Metals in Biology and Biocatalysis Through Biospectroscopy-1st TIMB3 Annual Meeting, 22-23 September 2019, 2019.
  17. Taborda, André; Brissos, Vânia; Martins, Lígia. Corresponding author: Martins, Lígia. "Improving a bacterial pyranose 2-oxidase through directed evolution for diagnosis biosensor applications". Paper presented in BIOTRANS 2019, 2019.
  18. Barbosa, Catarina; Silveira, M. Célia; Brissos, Vânia; Silva, Diogo; Martins, O. Ligia; Todorovic, Smilja. "Highly efficient hydrogen peroxide biosensors based on Pseudomonas putida dye decolourising peroxidase and engineered mutants". Paper presented in 1st Training School Chemistry of Metals in Biological Systems,12-19 May 2019, 2019.
  19. Monteiro, Hugo; Vieira, Marta; Hernandez, Guillem; González, Amaia; Borges, Patrícia; Frazão, Carlos; Monza, Emanuele; et al. "Dynamic Strucutral Studies ¿ From Pathogenic Disordered Proteins To Folded Relevant Enzymes". Paper presented in Twin to Illuminate Metals in Biology and Biocatalysis through Biospectroscopy (TIMB3), 2018.
  20. Brissos, Vânia; Pereira, Luciana. "Development of Direct Evolution Assays to Improve the Redox Potential of CotA-laccase.". Paper presented in European Science Foundation, ESF-EMBO Symposium: Protein Design and Evolution for Biocatalysis, 2008.
  21. Brissos, Vânia; Pereira, Luciana. Corresponding author: Brissos, Vânia. "Expression System of CotA-laccase for Directed Evolution and Validation of High-Throughput Screenings for the Oxidation of High-Redox Potential Dyes.". Paper presented in 4th European Meeting on Oxizymes, 2008.
Journal article
  1. Brissos, V.; Rénio, M.; Lejmel, M.A.; Estevinho, R.; Robalo, M.P.; Ventura, M.R.; Martins, L.O.. "Unlocking Lignin's Potential: Engineered Bacterial Laccases to Produce Biologically Active Molecules". ChemSusChem (2025): http://www.scopus.com/inward/record.url?eid=2-s2.0-85216234795&partnerID=MN8TOARS.
    10.1002/cssc.202401386
  2. Bárbara Mendes; Vânia Brissos; Lígia O. Martins; Felipe Conzuelo. "Enzyme-Modified Microelectrode for Simultaneous Local Measurements of O2 and pH". Analytical Chemistry (2024): https://doi.org/10.1021/acs.analchem.4c03150.
    10.1021/acs.analchem.4c03150
  3. Rizzo, F.; Brissos, V.; Villain, S.; Martins, L.O.; Conzuelo, F.. "Simple and Directed Immobilization of a Multicopper Oxidase on Flat Bare Gold Electrodes Provides High Catalytic Currents for O2 Reduction". ACS Catalysis 14 7 (2024): 4760-4767. http://www.scopus.com/inward/record.url?eid=2-s2.0-85188019471&partnerID=MN8TOARS.
    10.1021/acscatal.4c00516
  4. De Simone, M.; Alonso-Cotchico, L.; Lucas, M.F.; Brissos, V.; Martins, L.O.. "Distal mutations enhance efficiency of free and immobilized NOV1 dioxygenase for vanillin synthesis". Journal of Biotechnology 391 (2024): 92-98. http://www.scopus.com/inward/record.url?eid=2-s2.0-85196018897&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2024.06.012
  5. Brissos, V.; Borges, P.T.; Sancho, F.; Lucas, M.F.; Frazão, C.; Conzuelo, F.; Martins, L.O.. "Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases". Journal of Biological Inorganic Chemistry 29 3 (2024): 339-351. http://www.scopus.com/inward/record.url?eid=2-s2.0-85182463809&partnerID=MN8TOARS.
    10.1007/s00775-023-02040-y
  6. Giulia Moro; Vânia Brissos; Chiara Zanardi; Lígia O. Martins; Felipe Conzuelo. "Electrochemical investigations of the multicopper oxidase from Aquifex aeolicus under direct electron transfer with carbon electrodes". Electrochimica Acta (2023): https://doi.org/10.1016/j.electacta.2023.143199.
    10.1016/j.electacta.2023.143199
  7. De Simone, M.; Alvigini, L.; Alonso-Cotchico, L.; Brissos, V.; Caroli, J.; Lucas, M.F.; Monza, E.; et al. "Rationally Guided Improvement of NOV1 Dioxygenase for the Conversion of Lignin-Derived Isoeugenol to Vanillin". Biochemistry 62 2 (2023): 419-428. http://www.scopus.com/inward/record.url?eid=2-s2.0-85133535217&partnerID=MN8TOARS.
    10.1021/acs.biochem.2c00168
  8. Brissos, Vânia; Borges, Patrícia T.; Núñez-Franco, Reyes; Lucas, Maria Fátima; Frazão, Carlos; Monza, Emanuele; Masgrau, Laura; Cordeiro, Tiago N.; Martins, Lígia O.. "Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase". ACS Catalysis 12 9 (2022): 5022-5035. http://dx.doi.org/10.1021/acscatal.2c00336.
    10.1021/acscatal.2c00336
  9. Borges, P.T.; Silva, D.; Silva, T.F.D.; Brissos, V.; Cañellas, M.; Lucas, M.F.; Masgrau, L.; et al. "Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase". Computational and Structural Biotechnology Journal 20 (2022): 3899-3910. http://www.scopus.com/inward/record.url?eid=2-s2.0-85134879064&partnerID=MN8TOARS.
    10.1016/j.csbj.2022.07.032
  10. Rodrigues, Carolina F.; Borges, Patrícia T.; Scocozza, Magali F.; Silva, Diogo; Taborda, André; Brissos, Vânia; Frazão, Carlos; Martins, Lígia O.. "Loops around the Heme Pocket Have a Critical Role in the Function and Stability of BsDyP from Bacillus subtilis". International Journal of Molecular Sciences 22 19 (2021): 10862. http://dx.doi.org/10.3390/ijms221910862.
    Published • 10.3390/ijms221910862
  11. Barbosa, Catarina; Silveira, Célia M.; Silva, Diogo; Brissos, Vânia; Hildebrandt, Peter; Martins, Lígia O.; Todorovic, Smilja; Instituto de Tecnologia Química e Biológica António Xavier (ITQB). "Immobilized dye-decolorizing peroxidase (DyP) and directed evolution variants for hydrogen peroxide biosensing". (2020): http://hdl.handle.net/10362/92415.
    https://doi.org/10.1016/j.bios.2020.112055
  12. Borges, P.T.; Brissos, V.; Hernandez, G.; Masgrau, L.; Lucas, M.F.; Monza, E.; Frazão, C.; Cordeiro, T.N.; Martins, L.O.. "Methionine-Rich Loop of Multicopper Oxidase McoA Follows Open-to-Close Transitions with a Role in Enzyme Catalysis". ACS Catalysis 10 13 (2020): 7162-7176. http://www.scopus.com/inward/record.url?eid=2-s2.0-85087985255&partnerID=MN8TOARS.
    10.1021/acscatal.0c01623
  13. Blánquez, A.; Rodríguez, J.; Brissos, V.; Mendes, S.; Martins, L.O.; Ball, A.S.; Arias, M.E.; Hernández, M.. "Decolorization and detoxification of textile dyes using a versatile Streptomyces laccase-natural mediator system". Saudi Journal of Biological Sciences 26 5 (2019): 913-920. http://www.scopus.com/inward/record.url?eid=2-s2.0-85047422155&partnerID=MN8TOARS.
    10.1016/j.sjbs.2018.05.020
  14. Brissos, V.; Tavares, D.; Sousa, A.C.; Robalo, M.P.; Martins, L.O.. "Engineering a Bacterial DyP-Type Peroxidase for Enhanced Oxidation of Lignin-Related Phenolics at Alkaline pH". ACS Catalysis 7 5 (2017): 3454-3465. http://www.scopus.com/inward/record.url?eid=2-s2.0-85020919472&partnerID=MN8TOARS.
    10.1021/acscatal.6b03331
  15. Martins, L.O.; Durão, P.; Brissos, V.; Lindley, P.F.. "Laccases of prokaryotic origin: Enzymes at the interface of protein science and protein technology". Cellular and Molecular Life Sciences 72 5 (2015): 911-922. http://www.scopus.com/inward/record.url?eid=2-s2.0-84926635382&partnerID=MN8TOARS.
    10.1007/s00018-014-1822-x
  16. Brissos, V.; Ferreira, M.; Grass, G.; Martins, L.O.. "Turning a Hyperthermostable Metallo-Oxidase into a Laccase by Directed Evolution". ACS Catalysis 5 8 (2015): 4932-4941. http://www.scopus.com/inward/record.url?eid=2-s2.0-84938703788&partnerID=MN8TOARS.
    10.1021/acscatal.5b00771
  17. Brissos, Vania; Goncalves, Nadia; Melo, Eduardo; Martins, Ligia O.. "Improving kinetic or thermodynamic stability of an azoreductase by directed evolution". (2015): http://hdl.handle.net/10400.1/11650.
    10.1186/s13071-015-0771-z
  18. Santos, A.; Mendes, S.; Brissos, V.; Martins, L.O.. "New dye-decolorizing peroxidases from Bacillus subtilis and Pseudomonas putida MET94: Towards biotechnological applications". Applied Microbiology and Biotechnology 98 5 (2014): 2053-2065. http://www.scopus.com/inward/record.url?eid=2-s2.0-84896691646&partnerID=MN8TOARS.
    10.1007/s00253-013-5041-4
  19. Brissos, V.; Gonçalves, N.; Melo, E.P.; Martins, L.O.. "Improving kinetic or thermodynamic stability of an azoreductase by directed evolution". PLoS ONE 9 1 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84900338484&partnerID=MN8TOARS.
    10.1371/journal.pone.0087209
  20. Mendes, S.; Brissos, V.; Gabriel, A.; Catarino, T.; Turner, D.L.; Todorovic, S.; Martins, L.O.. "An integrated view of redox and catalytic properties of B-type PpDyP from Pseudomonas putida MET94 and its distal variants". Archives of Biochemistry and Biophysics (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84925764181&partnerID=MN8TOARS.
    10.1016/j.abb.2015.03.009
  21. Silva, C.S.; Damas, J.M.; Chen, Z.; Brissos, V.; Martins, L.O.; Soares, C.M.; Lindley, P.F.; Bento, I.. "The role of Asp116 in the reductive cleavage of dioxygen to water in CotA laccase: Assistance during the proton-transfer mechanism". Acta Crystallographica Section D: Biological Crystallography 68 2 (2012): 186-193. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863011701&partnerID=MN8TOARS.
    10.1107/S0907444911054503
  22. Brissos, V.; Chen, Z.; Martins, L.O.. "The kinetic role of carboxylate residues in the proximity of the trinuclear centre in the O 2 reactivity of CotA-laccase". Dalton Transactions 41 20 (2012): 6247-6255. http://www.scopus.com/inward/record.url?eid=2-s2.0-84860491041&partnerID=MN8TOARS.
    10.1039/c2dt12067d
  23. Brissos, V.; Pereira, L.; Munteanu, F.-D.; Cavaco-Paulo, A.; Martins, L.O.. "Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes". Biotechnology Journal 4 4 (2009): 558-563. http://www.scopus.com/inward/record.url?eid=2-s2.0-65549087033&partnerID=MN8TOARS.
    10.1002/biot.200800248
  24. Costa, L.; Brissos, V.; Lemos, F.; Ramôa Ribeiro, F.; Cabral, J.M.S.. "Enhancing the thermal stability of lipases through mutagenesis and immobilization on zeolites". Bioprocess and Biosystems Engineering 32 1 (2009): 53-61. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149092996&partnerID=MN8TOARS.
    10.1007/s00449-008-0220-x
  25. Costa, L.; Brissos, V.; Lemos, F.; Ribeiro, F.R.; Cabral, J.M.S.. "Comparing the effect of immobilization methods on the activity of lipase biocatalysts in ester hydrolysis". Bioprocess and Biosystems Engineering 31 4 (2008): 323-327. http://www.scopus.com/inward/record.url?eid=2-s2.0-44049097661&partnerID=MN8TOARS.
    10.1007/s00449-007-0165-5
  26. Brissos, V.; Melo, E.P.; Martinho, J.M.G.; Cabral, J.M.S.. "Biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant AOT". Biochimica et Biophysica Acta - Proteins and Proteomics 1784 9 (2008): 1326-1334. http://www.scopus.com/inward/record.url?eid=2-s2.0-53149145988&partnerID=MN8TOARS.
    10.1016/j.bbapap.2008.04.017
  27. Brissos, V.; Eggert, T.; Cabral, J.M.S.; Jaeger, K.-E.. "Improving activity and stability of cutinase towards the anionic detergent AOT by complete saturation mutagenesis". Protein Engineering, Design and Selection 21 6 (2008): 387-393. http://www.scopus.com/inward/record.url?eid=2-s2.0-44649102734&partnerID=MN8TOARS.
    10.1093/protein/gzn014
  28. Costa, L.; Brissos, V.; Lemos, F.; Ribeiro, F.R.; Cabral, J.M.S.. "Following multi-component reactions in liquid medium using spectral band-fitting techniques". Applied Spectroscopy 62 8 (2008): 932-935. http://www.scopus.com/inward/record.url?eid=2-s2.0-50149098977&partnerID=MN8TOARS.
Online resource
  1. Borges, Patricia T.; Brissos, Vânia; Carlos Frazao; Martins, Lígia O.. Crystal structure of a multicopper oxidase 3F3 variant from Pyrobaculum aerophilum. 2024. https://www.rcsb.org/structure/unreleased/8P4G.
  2. Borges, Patricia T.; Rodrigues, C..; Silva, D..; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia. Crystal structure of a DyP-type peroxidase from Bacillus subtilis in P3121 space group. 2021. https://www.rcsb.org/structure/7PKX.
  3. Borges, Patricia T.; Rodrigues, C..; Silva, D.,; Taborda, André; Brissos, Vânia; Carlos Frazao; Martins, Lígia. Crystal structure of a DyP-type peroxidase 5G5 variant from Bacillus subtilis. 2021. https://www.rcsb.org/structure/7PL0.
  4. Borges, Patricia T.; Brissos, Vânia; Cordeiro, Tiago N.; Martins, Lígia; Carlos Frazao. Crystal structure of McoA multicopper oxidase 2F4 variant from the hyperthermophile Aquifex aeolicus. 2021. https://www.rcsb.org/structure/6TTD.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/07 Enzymatic synthesis of lignans and neo-lignans using hyperthermophilic engineered laccase Oxizymes
(Lublin, Poland)
2024/07 Improving NOV1 oxygenase for high-yield production of lignin-derived vanillin by proximal and distal mutations Oxizymes
(Lublin, Poland)
2024/07 An enzyme-modified microelectrode for simultaneous O2 and pH measurements 19th International Conference on Electroanalysis
(Ulm, Germany)
2023/09 Electrochemical characterizations of the Aquifex aeolicus metallo-oxidase McoA 74th Annual Meeting of the International Society of Electrochemistry
(Lyon, France)
2023/08 Electrochemical investigations of new multicopper oxidases: The Aquifex aeolicus McoA metallo-oxidase XXV Meeting of the Portuguese Electrochemical Society
(Coimbra, Portugal)
2022 Allosteric Mutations Shape Substrate-Binding Sites During Evolution of a Metallo-Oxidase into a Laccase Oxizymes
(Siena, Italy)
2021 Flash presentation on His515, adjacent to copper centres of Aquifex aeolicus McoA, is a determinant of the enzyme activity and stability EFB2021 Virtual Conference
European Federation of Biotechnology
2021 Structural analysis of a laboratory evolution variant of hyperthermophilic Multicopper Oxidase McoA ESRF User meeting
2019 New ligninolytic enzymes by directed evolution Microbiotech19
(Coimbra, Portugal)
2018 Improving a bacterial pyranose 2-oxidase using a combination of rational and directed evolution for biosensor application 18th European Congress of Biotechnology
(Geneva, Switzerland)
2018 Engineering bacterial ligninolytic enzymes for lignin recalcitrance 15th European Workshop on Lignocellulosics and Pulp
(Aveiro, Portugal)
2018 Multidirectional comparative analysis between a laccase variant design by directed evolution and the native mettalo-oxidase Oxizymes
(Belfast, Ireland)
2018 Evolving bacterial laccases and DyP-peroxidases for oxidation of lignin-related phenolics Lignobiotech
(Helsinki, Finland)
2017 Improving a bacterial DyP-type peroxidase for lignin-related phenolics oxidation at alkaline pH using directed evolution Microbiotec
(Porto, Portugal)
2017 Comparison of activity of Pseudomonas putida PpDyP peroxidase and of its evolved variant 6E10 for 28 lignin-related phenolics Microbiotec
(Porto, Portugal)
2017 Design of a competitive toolbox of engineered enzymes for lignin degradation and valorization COST Action 1306, WG 4 Meeting
(Lulea, Switzerland)
2016 Improved bacterial DyP peroxidase from Pseudomonas putida MET94 for aromatic compound degradation 4th Symposium on Biotechnology Applied to Lignocelluloses
(Madrid, Spain)
2016 Directed evolution of PpDyP, a bacterial DyP-type peroxidase, for improved oxidation of phenolic compounds 5th International Conference on Novel Enzymes
(Groningen, Netherlands)
2016 Directed evolution of bacterial lignolytic enzymes COST Action FP1306, Joint WG1 & WG3 Meeting
(Lisboa, Portugal)
2015 Engineering enzymatic specificity of ligninolytic enzymes FP1306 COST Action. Joint WG1 & WG3 Meeting. COST
(Litinov, Czech Republic)
2014 Molecular Mechanisms in laccases: insights from structural and simulation studies 4th International Conference on Novel Enzymes,
(Gent, Belgium)
2014 Improving themostability of an azoreductase from Pseudomonas putida by directed evolution Oxizymes
(Vienna)
2013 Stabilization of FMN-dependent NADPH:dye/quinone reductase from Pseudomonas putida by directed evolution 9th European Biophysics Congress
(Lisboa, Portugal)
2013 Catalytic and spectroscopic characterization of two bacterial dye-decolourising peroxidases 9th European Biophysics Congress
(Lisboa, Portugal)
2012 The role of carboxylate residues in the proximity of the trinuclear centre in the O2 reactivity of CotA-laccase Oxizymes,
(Marseille, France)
2008 Copper Incorporation into Recombinant CotA-Laccase from Bacillus subtilis 4th European Meeting in Oxizymes
(Helsinki, Finland)
2008 Monitoring multi-component liquid reaction systems containing highly dispersible heterogeneous catalysts using in situ diode array spectrophotometry and band-fitting techniques 10th International Chemical and Biological Engineering Conference
(Braga, Portugal)
2007 Kinetic Modeling of Ester Hydrolysis Catalyzed by Free and Immobilized Lipases and Monitored by In Situ Diode Array Spectrophotometry EuropacatVIII
(Turku, Finland)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/04/01 - 2022/09/30 Directed evolution of McoP, a muticopper oxidase from Aquifex aeolicus, for lignin biotransformation
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/09/01 - 2021/06/30 Tailor BsDyP for Biorefinery Applications using a Laboratory Enzyme Evolution Approach
Co-supervisor
 Biotechnology for Sustainability (Master)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/09/01 - 2021/01 Biorefineries: Production of Pharmaceutical and Nutraceutical Bio-products
Co-supervisor
 Mestrado em Biotecnologia, FCT, Universidade Nova de Lisboa (Master)
2018/06 - 2019/09 Directed evolution of McoA, a muticopper oxidase from Aquifex aeolicus, for lignin biotransformation
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017/11 - 2018/06 Directed evolution of McoA, a muticopper oxidase from Aquifex aeolicus, for lignin biotransformation
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018/01 - 2018/05 Oxidase de Multicobre McoP da arquebactéria Pyrobaculum aerophilum: Evolução Dirigida de Metalo-oxidase a Lacase
Co-supervisor
 Biologia Celular e Molecular (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/01 - 2018/05 Técnicas de evolução dirigida e biologia sintética aplicadas à enzima PpDyP, uma peroxidase de Pseudomonas putida
Co-supervisor
 Biologia Celular e Molecular (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/02 - 2017/06 Directed evolution of PpDyP, a dye-decolourising peroxidases from Pseudomonas putida
Co-supervisor
 Bioquímica (Degree)
Universidade de Évora Escola de Ciências e Tecnologia, Portugal
2015/05 - 2016/12 Directed evolution of multicopper oxidases for lignin biotransformation
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2016/01 - 2016/06 Optimization of a new fluorescent assay to detect hydrogen peroxide
Co-supervisor
 Bioquímica (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014/02 - 2015/11 Bacterial hydrogen peroxide forming enzymes
Co-supervisor
 Engenharia Biológica (Master)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal
2014/06 - 2015/03 Isolate and identify new bacterial lignin-degrading strains and identify the key enzymes
Co-supervisor
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013/09 - 2014/12 Engineering new bacterial dye-decolourising peroxidases for lignin degradation
Co-supervisor
 Microbiologia Aplicada (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2014/01 - 2014/05 Cloning and heterologous expression of new radical copper oxidases from the soil bacteria Arthrobacter siccitolerans
Co-supervisor
 Bioquímica (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014/01 - 2014/05 Improving whole cell systems for Dye type peroxidases production and immobilization
Co-supervisor
 Bioquímica (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/10 - 2012/12 Directed evolution of McoA, a metallo-oxidase from Aquifex aeolicus
Co-supervisor
 Bioquímica (Degree)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012/02 - 2012/09 Directed evolution of McoA, a metallo-oxidase from Aquifex aeolicus
Co-supervisor
 Bioquímica (Degree)
Universidade de Évora Escola de Ciências e Tecnologia, Portugal
2011/08 - 2012/09 Improve PpAzoR azoreductase thermostability through directed evolution approaches
Co-supervisor
 Bioquímica (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2023 - 2023 ITQB Open Day (2023 - 2023)
Exhibition (Member of the Organising Committee)
 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2015 - 2015 Green-IT General Meeting (2015)
Meeting (Member of the Organising Committee)
 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013 - 2013 Staff Member 9th European Biophysics Congress (2013 - 2013)
Congress (Other)
 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2024/10 - 2024/10 Grant Writing Course Grant Writing Course, led by Dr. Thiago Carvalho and Dr. David del Álamo
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2024/07/15 - 2024/07/15 ITQB SummerScience (Meet Students and Scientists) ITQB SummerScience (Meet Students and Scientists)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2024 - 2024 Chair in a session of the 14th ITQB NOVA PhD Students' Meeting
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2023/10/19 - 2023/10/19 Chair the "Responsible Consumption and Production" session of the 13th ITQB NOVA PhD Students' Meeting 13th ITQB NOVA PhD Students' Meeting
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2023/07/17 - 2023/07/17 ITQB SummerScience (Meet Students and Scientists) ITQB SummerScience
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/01 - 2020/06 Startup Research
Other
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade Nova de Lisboa NOVA SBE Venture Lab, Portugal
2019/09/27 - 2019/09/27 Noite Europeia dos Investigadores
Festival
2019 - 2019 Statistics Course (RStudio and R)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017/09/29 - 2017/09/29 Noite Europeia dos Investigadores
Festival
2015/04/10 - 2015/04/11 Festival de Biotecnologia
Festival
2012 - 2012 Science Writing Course for PhDs led by Dr. Andrea Gwosdow
Other
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010 - 2010 Comparative Genomics and Metagenomics, Impacts on Health and Environment
Other
Universidad de Granada, Spain
2009 - 2009 8th short course of the Portuguese Biophysical Society “Systems Biology”
Portuguese Biophysical Society , Portugal
2008/11/22 - 2008/11/23 Fórum Ciência Viva
Festival
2006 - 2006 Practical course on Directed Enzyme Evolution
Other
Institute of Biotechnology, EU Centre of Excellence, Lithuania

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2021/02/03 Development of a new biophotocatalytic system for biofuel production
(Thesis) Main arguer
 Catarina Teixeira Toste (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015 Aprovechamiento de su potencial oxidativo para la degradación de contaminantes ambientales
(Thesis) Main arguer
 Alba Blánquez Moya (PhD)
Universidad de Alcalá de Henares, Spain

Committee member

Activity description
Role 		Institution / Organization
2023 - Current Member of the thesis committee of Susana Margarida Marques Costa, "Unveiling the relevance of the interplay between ncRNAs and ribonucleases in biotechnologically relevant bacteria". PhD in Molecular Biosciences , ITQB-NOVA, Portugal
Member
 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2017 - 2022 Biocatalysis Química Sustentável (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017/11 - 2021/03 Engenharia Genética Avançada Engenharia Biológica e Química (Mestrado integrado) Instituto Politécnico de Setúbal Escola Superior de Tecnologia do Barreiro, Portugal
2018 - 2021 Biocatalysis for sustainability Biotecnologia para a Sustentabilidade (Mestrado integrado) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Mentoring / Tutoring

Topic Student name
2024/01 - Current Characterization of a new lactate dehydrogenase from Porcellio dilatatus gut microbiome Catarina Coelho, PhD student
2024/02 - 2024/04 Reversal of Enantioselectivity in Phenylalanine Ammonia-Lyase through Directed Evolution Raluca-Bianca Tomoiaga, PhD student
2013/04 - 2013/07 Production, purification and biochemical characterization of the recombinant bacterial laccase SilA from Streptomyces ipomoea CECT 3341 Alba Blánquez, PhD Student

Other jury / evaluation

Activity description Institution / Organization
2015 - Current Reviewer of Microbial cell factories (Q1, IF: 4.669), Applied and Environmental Microbiology (Q1, IF: 3.807), PlosOne (Q1, IF: 3.54), Applied Microbiology and Biotechnology (Q1, IF: 3.670), New Biotechnology (Q1, IF: 3.183), Archives of Biochemistry and Biophysics (Q2, IF: 3.017), Enzyme and Microbial Technology (Q2, IF: 2.932), FEBS Open bio (Q2, IF: 2.101), Journal of Applied Microbiology (Q2, IF: 2.099)
Distinctions

Other distinction

2015 Post-Doc grant (SFRH/BPD/109431/2015)
Fundação para a Ciência e a Tecnologia, Portugal
2011 Travel grant to the Biotransformation 2011 Summer School
2008 Post-Doc grant (SFRH/BPD/46808/2008)
Fundação para a Ciência e a Tecnologia, Portugal
2006 Travel grant to the practical course on Directed Evolution, Institute of Biotechnology, EU Centre of Excellence, Vilnius, Lithuania
2005 Travel grant to the Biotrans 2005 Symposium, Delft University of Technology, The Netherlands
2002 PhD grant (SFRH/BD/9019/2002)
Fundação para a Ciência e a Tecnologia, Portugal