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I have extensive knowledge and skills in both molecular and cell biology, ranging from basic techniques such as recombinant DNA and Western Blot, to more advanced techniques like CRISPR/Cas9 technology. Additionally, I have valuable experience in both confocal and live-imaging microscopy. My work has focused on investigating membrane contact sites and analyzing the dynamics of different organelles at these regions, including ER, lysosomes, mitochondria, and lipid droplets. Additionally, my research has delved into molecular neurosciences, exploring the mechanisms underlying neurodegenerative diseases such as Alzheimer's disease (via the study of the APP gene) and Parkinson’s disease (via the study of the VPS13C gene). I have contributed to the field by designing and developing new tools, including KO cell lines, plasmids encoding tagged proteins, and a polyclonal antibody, which through extensive validation proved to vastly outperform the antibodies commercially available.
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Identification

Personal identification

Full name
Roberto Alexandre dos Santos Dias

Citation names

  • Dias, Roberto A
  • dos Santos Dias, Roberto
  • Dias, RA

Author identifiers

Ciência ID
4F14-1A8D-EA67
ORCID iD
0000-0002-3236-6061

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Proficiency (C2) Advanced (C1) Proficiency (C2) Advanced (C1)
Education
Degree Classification
2013/04/01 - 2017/10/24
Concluded
 Biomedicina (Doutoramento)
Universidade de Aveiro, Portugal

Universidade NOVA de Lisboa, Portugal
"Caracterização do papel da GaO na Neuritogénese: um destaque para o complexo Gao-Proteína precursora de amilóide" (THESIS/DISSERTATION)
2010/09 - 2012/07
Concluded
 Biomedicina Molecular (Mestrado integrado)
Universidade de Aveiro, Portugal
"APP phosphorylation on APP/Gao colocalization and STAT3 activation" (THESIS/DISSERTATION)
2007/09 - 2010/07
Concluded
 Ciências Biomédicas (Licenciatura)
Major in Biomedicina Molecular
Universidade de Aveiro, Portugal
 16
Affiliation

Science

Category
Host institution 		Employer
2024/06 - Current Auxiliary Researcher (Research) Universidade de Coimbra, Portugal
Universidade de Coimbra Multidisciplinary Institute of Ageing, Portugal
2022/06 - 2024/04 Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
(...)
2018/04 - 2022/04 Postdoc (Research) Associatie KU Leuven, Belgium
VIB KU Leuven Center for Brain & Disease Research, Belgium
2012/09 - 2013/03 Research Assistant (Research) Universidade de Aveiro, Portugal
Universidade de Aveiro, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2013/02 - 2014/01 Assistant (University Teacher) Universidade de Aveiro, Portugal
Projects

Grant

Designation Funders
2013/04/01 - 2017/03/31 ESTABLISHING G&O AS A PLASTICITY MARKER AND NEUROREGENERATIVE PROTEIN
SFRH/BD/90996/2012
Universidade de Aveiro, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2018/06/22 - 2022/06/21 GoBack - Novos alvos terapeuticos para lesoes medulares
PTDC/CVT-CVT/32261/2017
Universidade de Aveiro, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2020/01/01 - 2022/04/30 Unraveling how VPS13C deficiency affects interorganellar lipid transport and lysosomal homeostasis and how this contributes to the neuropathogenesis of Dementia with Lewy Bodies
G0C3620N
Post-doc
VIB KU Leuven Center for Brain & Disease Research, Belgium
Concluded
2019/01/01 - 2019/12/31 Institute for Biomedicine - Aveiro
UID/BIM/04501/2019
Universidade de Aveiro, Portugal

Universidade de Aveiro Instituto de Biomedicina, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Conference poster
  1. Dias, Roberto A; Gaspar-Silva, Filipa; Milosevic, Ira; Raimundo, Nuno. "The Impact of Nutrient Sensing on Lysosome- mitochondria Contact Sites". Paper presented in MIA Spring School, 2024.
  2. Dias, Roberto A; Gaspar-Silva, Filipa; Milosevic, Ira; Raimundo, Nuno. "Role of Lysosome-Mitochondria Membrane Contact Sites in Nutrient Sensing". Paper presented in IV Metabolism, Ageing and Disease (MAD) Meeting, 2024.
  3. DIAS, ROBERTO; Gaspar-Silva, Filipa; Milosevic, Ira; Raimundo, Nuno. "Role of Lysosome-Mitochondria Membrane Contact Sites in Nutrient Sensing". Paper presented in 3 rd RESETageing Conference, 2023.
  4. Dias, Roberto A; Trindade, Dário; Vieira, Sandra Isabel. "The Alzheimer¿s amyloid protein APP and G(alpha)o physically and functionally interact to promote neuritogenesis". Paper presented in FENS Forum 2022. Paris, France, 2022.
  5. DIAS, ROBERTO; Leroy, Thomas; Annaert, Wim. "Exploring the molecular and cellular functions of VPS13C". Paper presented in CBD Science Day, 2021.
  6. DIAS, ROBERTO; Cerqueira, Ana Regina; Ferreira JV; da Rocha, J.F.; Pereira, Paulo; da Cruz e Silva, Odete; Vieira, Sandra Isabel. "Regulation of Gao and APP protein levels". Paper presented in EMBO Proteostasis, 2017.
  7. Dias, Roberto A; Cerqueira, AR; da Rocha, J.F.; da Cruz e Silva, Odete; Vieira, Sandra Isabel. "Gao role in neuronal differentiation". Paper presented in Research Day@UA, 2016.
  8. Dias, Roberto A; da Rocha, J.F.; Cerqueira, A.R.; Pinho, A.C.; da Cruz e Silva, Odete; Vieira, Sandra Isabel. "The Alzheimer’s amyloid precursor protein modulates neuritogenic and migration processes relevant to neuroregeneration". Paper presented in EMBO The molecular and cellular basis of regeneration and tissue repair, 2014.
  9. Dias, Roberto A; Cerqueira, A.R.; da Rocha, J.F.; da Cruz e Silva, Odete; Vieira, Sandra Isabel. "The influence of APP S655 phosphorylation in APP/Gao subcellular colocalization". Paper presented in 6th National Meeting on Cell Signaling, 2012.
Journal article
  1. Vieira, Sandra I.; Nunes da Silva, Raquel; Alves, Mariana; DIAS, ROBERTO; Meireles Sousa, Ana M.; Camões, Fatima; Maia, André; et al. "Liprobe, a vital dye for lipid aggregates detection in imaging and high-content screens". Frontiers in Photonics 3 (2022): http://dx.doi.org/10.3389/fphot.2022.963778.
    10.3389/fphot.2022.963778
  2. Smolders, Stefanie; Philtjens, Stéphanie; Crosiers, David; Sieben, Anne; Hens, Elisabeth; Heeman, Bavo; Van Mossevelde, Sara; et al. Corresponding author: Van Broeckhoven, Christine. "Contribution of rare homozygous and compound heterozygous VPS13C missense mutations to dementia with Lewy bodies and Parkinson's disease.". Acta neuropathologica communications (2021): https://doi.org/10.1186/s40478-021-01121-w.
    10.1186/s40478-021-01121-w
  3. de Oliveira, Patrícia G.; Ramos, Marta L. S.; Amaro, António J.; DIAS, ROBERTO; Vieira, Sandra Isabel. Corresponding author: Vieira, Sandra Isabel. "Gi/o-Protein Coupled Receptors in the Aging Brain.". Frontiers in aging neuroscience (2019): https://doi.org/10.3389/fnagi.2019.00089.
    10.3389/fnagi.2019.00089
  4. Martins SA; Correia PD; Dias RA; da Cruz E Silva OAB; Vieira SI. "CD81 Promotes a Migratory Phenotype in Neuronal-Like Cells.". Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada (2019): http://europepmc.org/abstract/med/30714554.
    10.1017/s1431927618015532
  5. Dias, R.A.; Gonçalves, B.P.; da Rocha, J.F.; da Cruz e Silva, O.A.B.; da Silva, A.M.F.; Vieira, S.I.. "NeuronRead, an open source semi-automated tool for morphometric analysis of phase contrast and fluorescence neuronal images". Molecular and Cellular Neuroscience 85 (2017): 57-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-85028572236&partnerID=MN8TOARS.
    10.1016/j.mcn.2017.08.002
  6. Pinho, A.C.; Dias, R.; Cerqueira, A.R.; Da Cruz E Silva, O.A.B.; Vieira, S.I.. "APP and its secreted fragment sAPP in SH-SY5Y neuronal-like migration". Microscopy and Microanalysis 21 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84969765239&partnerID=MN8TOARS.
    10.1017/S1431927614013853
Thesis / Dissertation
  1. "Characterization of Gao role on neuritogenesis: a focus on the Gao-Amyloid precursor protein complex". PhD, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22057.
  2. "APP phosphorylation on APP/Gao colocalization and STAT3 activation". Master, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/10819.

Intellectual property

Patent
  1. Silva, Artur; Samuel J. A. Guieu; João C. M. C. G. Rocha; Sandra I. M. O. Vieira; da Cruz e Silva, Odete; DIAS, ROBERTO; Raquel F. Ruivo. 2023. "Fluorescent compounds, production methods and uses thereof".
    Granted/Issued
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/01/31 Lysosome: Mitochondria Contact Sites in Nutrient Sensing DCV.talks
Departamento de Ciências da Vida, Universidade de Coimbra (Coimbra, Portugal)
2017/09/21 NeuronRead, an open source tool for morphometric analysis of PhC (and fluorescence) neuronal images III Quantitative Fluorescence Microscopy Course
Centro de Neurociências e Biologia Celular, Universidade de Coimbra (Coimbra, Portugal)
2017/06/07 NeuronRead, an open source tool for morphometric analysis of neuronal images III iBiMED postgrad symposium
iBiMED, Universidade de Aveiro (Aveiro, Portugal)
2016/06/29 Gao as a key player in neuronal differentiation II iBiMED postgrad symposium
iBiMED, Universidade de Aveiro (Aveiro, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021/09 - 2022/12 Effects of CB1R activation on STAT3 and ERK1/2 pathways, and on the neuritogenesis mediated by APP and its phosphorylation
Co-supervisor
 Biomedicina Molecular (Master)
Universidade de Aveiro, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2015 - 2015 Scientific monitor of the "Academia de Verão", edition of 2015 (2015/07/05 - 2015/07/10)
Other (Member of the Organising Committee)
 Universidade de Aveiro, Portugal
2013 - 2013 7th National Meeting on Cell Signaling - SINAL 2013 (2013/12/06 - 2013/12/07)
Congress (Member of the Organising Committee)
 Universidade de Aveiro, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2023/12/13 Characterization of BRI2 expression and localization during neuronal differentiation
(Thesis) Main arguer
 Ruslana Savchuk (Master)
Universidade de Aveiro, Portugal
2023/11/20 Impact of Gao mutations on neuritogenesis induced by Gao and the Alzheimer's Amyloid Precursor Protein (APP)
(Thesis) Main arguer
 ALEXANDRE LEITÃO PEREIRA (Master)
Universidade de Aveiro, Portugal
Distinctions

Award

2017 Best Oral Communication
Universidade de Aveiro Instituto de Biomedicina, Portugal

Other distinction

2018 Marie Sklodowska-Curie Actions Seal of Excellence
European Commission Marie Sklodowska-Curie Actions, Belgium
2012 PhD Scholarship
Fundação para a Ciência e a Tecnologia, Portugal