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Identification

Personal identification

Full name
Maria Helena Guimarães Bastos de Carvalho

Author identifiers

Ciência ID
4F10-03A4-DE22
ORCID iD
0000-0003-0651-7601

Email addresses

  • helenacarvalho@cibio.up.pt (Professional)

Addresses

  • CIBIO - Centro de Investigação em Biodiversidade e Recursos Genéticos/ InBIO Laboratório Associado, Universidade do Porto Campus Agrário de Vairão Rua Padre Armando Quintas , 4485-661 , Vairão , Vila do Conde, Portugal (Professional)

Websites

Education
Degree Classification
2009 - 2009
Concluded
 Partek Genomics Suite training (Pós-Graduação)
Major in Bioinformática
Instituto Gulbenkian de Ciência, Portugal
2006 - 2006
Concluded
 GLIP Transcriptomics Bioinformatics Course (Pós-Graduação)
Major in Bioinformática
Fachhochschule der Wirtschaft - Bielefeld, Germany
1999/11
Concluded
 Instituto de Biologia Molecular e Celular (Doutoramento)
Major in Biologia Molecular de Plantas
Universidade do Porto Faculdade de Ciências, Portugal
"Studies on the cytosolic Glutamine Synthetase Isoenzymes and Genes of Medicago truncatula" (THESIS/DISSERTATION)
 Unanimidade
1997 - 1997
Concluded
 Recent Advances in Plant Developmental Biology as Considered from Physiological, Cellular and Molecular perspectives, (Pós-Graduação)
Major in Plant Biology
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1992
Concluded
 Provas de Acesso á categoria de Assistente de Investigação (Outros)
Major in Biologia Molecular de Plantas
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Muito Bom
1991 - 1991
Concluded
 Genetic Expression in Plant Cells (Pós-Graduação)
Major in Biologia Molecular e Celular
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1990 - 1990
Concluded
 Methods in Plant Cell Biology (Pós-Graduação)
Major in Methods in Plant Cell Biology
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1990 - 1990
Concluded
 Gene Cloning II- Antibodies and Expression Systems (Pós-Graduação)
Major in Biologia Molecular
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1989 - 1989
Concluded
 Protein Purification - the state of art (Pós-Graduação)
Major in Protein Purification
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1989 - 1989
Concluded
 Plant Secondary Messengers - Calcium and Calmodulin (Pós-Graduação)
Major in Plant Secondary Messengers
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1988 - 1988
Concluded
 Plant Virology (Pós-Graduação)
Major in Plant Virology
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1988 - 1988
Concluded
 Membrane Biochemistry (Pós-Graduação)
Major in Membrane Biochemistry
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1988 - 1988
Concluded
 Introdução à Análise Numérica (Pós-Graduação)
Major in Estatística
Universidade do Porto Departamento de Botânica, Portugal
1988 - 1988
Concluded
 Planeamento Estatístico de Experiências (Pós-Graduação)
Major in Estatística
Universidade do Porto Departamento de Botânica, Portugal
1988 - 1988
Concluded
 Gene Cloning I (Pós-Graduação)
Major in Biologia Molecular
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1983 - 1986
Concluded
 Biologia (Licenciatura)
Major in Ramo Científico e Técnológico
Universidade do Porto Faculdade de Ciências, Portugal
Affiliation

Science

Category
Host institution 		Employer
2015/08/13 - Current Principal Investigator (Research) Universidade do Porto Centro de Investigação em Biodiversidade e Recursos Genéticos, Portugal
2007/05/01 - 2015/08/13 Principal Investigator (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1999/11/21 - 2007/05/09 Auxiliary Researcher (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1992/03/28 - 1999/11/21 Research Assistant (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2013/02/01 - Current Invited Associate Professor (University Teacher) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Outputs

Publications

Book chapter
  1. Carvalho, Helena; Seabra, Ana Rita. "Inhibition of glutamine synthetase leads to a fast transcriptional activation of defence responses in root nodules.". In The Model Legume Medicago truncatula. Wiley, 2019.
    Published
  2. Carvalho, Helena. "A Célula Vegetal". In Biologia Molecular e Celular, 520-552. Portugal: Lidel, 2012.
    Published
  3. Carvalho, Helena; Julie Cullimore. "Regulation of glutamine synthetase isoenzymes and genes in the model legume Medicago truncatula". In Frontiers on Recent Developments in Plant Science, 157-175. BENTHAM SCIENCE PUBLISHERS, 2003.
    Published
  4. Carvalho, Helena. "Regulation of cytosolic glutamine synthetase in the model legume Medicago truncatula". In Nitrogen in a sustainable ecosystem: from the cell to the plant, 133-138. Backhuys Publishers, 2000.
    Published
Conference poster
  1. Carvalho, Helena; Seabra, Ana R; Liliana S. Silva. Corresponding author: Carvalho, Helena. "A non-conserved mechanism controlling seed development.". Paper presented in XVIII Portuguese-Spanish Congress on Plant Biology, 2023.
  2. Ana Rita Seabra; Liliana Silva; Anette Garrido; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Functional Divergence within the Glutamine Synthetase Type I Protein Family of Medicago truncatula". Paper presented in Forth International Symposium on the Nitrogen Nutrition of Plants, 2019.
  3. Ana Rita Seabra; Nuno Gonçalves; Isabel Abreu ; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Sumoylation of the plastid located glutamine synthetase (MtGS2a) in Medicago truncatula". Paper presented in Plant Biology Europe EPSO/FESPB, 2016.
  4. Mariana Alves ; Liliana Silva; Ana Seabra ; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Regulation of the plastid located glutamine synthetase by s-nitrosylation in Medicago truncatula.". Paper presented in 12th International Conference on Reactive Oxygen and Nitrogen Species in plants: from model plants to field, 2015.
  5. Liliana Silva; Ana Seabra ; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Glutamine synthetase is differentially nitrated during root nodule development in Medicago truncatula.". Paper presented in 12th International Conference on Reactive Oxygen and Nitrogen Species in plants: from model plants to field, 2015.
  6. Liliana Silva; Ana Seabra ; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Nitric oxide-mediated regulation of the four Glutamine Synthetase isoenzymes of Medicago truncatula.". Paper presented in 12th International Conference on Reactive Oxygen and Nitrogen Species in plants: from model plants to field, 2015.
  7. Liliana Silva; Paula Melo; Mariana Alves ; Carvalho, Helena. Corresponding author: Carvalho, Helena. "The plastid-located glutamine synthetase is regulated by S- Nitrosylation in Medicago truncatula". Paper presented in 5th NO plant Club Meeting, 2014.
  8. Liliana Silva; Ana Seabra; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Participation of the Medicago truncatula seed-specific glutamine synthetase (MtGS2b) in storage protein synthesis.". Paper presented in Plant Biology Europe FESPB/EPSO Congress, 2014.
  9. Liliana Silva; Nuno Leitão; Ana Seabra; Carvalho, Helena. "Possible role of Glutamine synthetase of the prokaryotic type in nitrogen signalling in Medicago truncatula". Paper presented in Second International Symposium on the Nitrogen Nutrition of Plants., 2013.
  10. Liliana Silva; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Involvement of Glutamine synthetase in the NO signaling response in root nodules of Medicago truncatula.". Paper presented in 18th International Congresso of Nitrogen Fixation. October 14-18, Miyazaki, Japan., 2013.
  11. Liliana Silva; Ana Seabra; Carvalho, Helena. "Expression of GSI-like genes in root nodules of Medicago truncatula". Paper presented in 10th European Nitrogen Fixation Conference, September 2-5, Munich, Germany., 2012.
  12. Paula Melo; Liliana Silva; Sofia Venade ; Carvalho, Helena. "Plastidic glutamine synthetase from Medicago truncatula is regulated by S-nitrosylation.". Paper presented in 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants, July 5-8, Budapest, Hungary, 2011.
  13. Ana Seabra; Patrícia Pereira; Jorg Becker ; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprogramming in Medicago truncatula root nodules". Paper presented in Model Legume Congress 2011, May 15-19, Sainte-Maxime, France., 2011.
  14. Liliana Silva; Ana Rita Seabra; Paula Melo; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Identification of a glutamine synthetase putative phosphorylating kinase from Medicago truncatula.". Paper presented in XVII Congresso Nacional de Bioquímica, December 15-17, Porto, Portugal., 2010.
  15. Paula Melo; Ana Seabra; Liliana Silva; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Regulation of glutamine synthetase by reactive nitrogen species un root nodules of medicago truncatula.". Paper presented in XVII Congresso Nacional de Bioquímica, December 15-17, Porto, Portugal., 2010.
  16. Ana Seabra; Patrícia Pereira; Jorg Becker ; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprogramming in medicago truncatula root nodules.". Paper presented in XVII Congresso Nacional de Bioquímica, December 15-17, Porto, Portugal., 2010.
  17. Ana Seabra; Patrícia Pereira; Jorg Becker ; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Transcriptome analysis of Medicago truncatula root nodules following inhibition of glutamine synthetase by phosphinothricin.". Paper presented in 9th European Nitrogen Fixation Conference, September 6-10. Geneve, Switzerland., 2010.
  18. Liliana Silva; Ana Rita Seabra; Paula Melo; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Characterization of a glutamine synthetase phosphorylating kinase isolated from root nodules of Medicago truncatula". Paper presented in 9th European Nitrogen Fixation Conference, September 6-10. Geneve, Switzerland., 2010.
  19. Pereira P.A, Seabra A ,Hohnjec N.,Balestrini R. ,Limpens E., Bonfante P.,Küster H, Carvalho H and Becker J.D. "The Symbiotic Genetic Programme of the Model Legume Medicago truncatula.". Paper presented in XXXV Portuguese Genetics Conference. Braga, Portugal, 2010.
  20. Ana Campilho; Carvalho, Helena. "Characterization of an AHP6 orthologue in Medicago truncatula roots.". Paper presented in 20th International Conference in Plant Growth Substances. Tarragona, Spain., 2010.
  21. Ana Rita Seabra, Julie Cullimore and Helena Carvalho. "Overexpression of glutamine synthetase in root nodules of Medicago truncatula leads to enhanced nitrogen utilization efficiency.". Paper presented in XVI Congress of the Federation of European Societies of Plant Biology (FESPB). Tampere, Finland., 2008.
  22. Isa Ribeiro, Ana Rita Seabra, Helena Carvalho, Paula Melo ; Ribeiro, Isa; Melo, Paula; Seabra, Rita and Carvalho, Helena. "In vitro inactivation of plant glutamine synthetase by tyrosine nitration.". Paper presented in XVI Congress of the Federation of European Societies of Plant Biology (FESPB). Tampere, Finland., 2008.
  23. 22. Ana Rita Seabra, Carlos Fernández-Tornero, Guy Schoehn, Pedro J. B. Pereira, Helena Carvalho. "Structural features of glutamine synthetase from Medicago truncatula". Paper presented in XVI Congress of the Federation of European Societies of Plant Biology (FESPB). Tampere, Finland., 2008.
  24. Ana Rita Seabra; Carvalho, Helena. Corresponding author: Carvalho, Helena. "Simultaneous 15N/13C labelling indicates that rhizobial nitrogen fixation is influenced by the levels of glutamine synthetase in root nodules.". Paper presented in 16º Congresso Nacional de Bioquímica. Açores, Portugal., 2008.
  25. Ana Rita Seabra, Philippe Thoquet, Julie Cullimore, Helena Carvalho. "Assessment of the role of glutamine synthetase in the control of nitrogen metabolism in root nodules.". Paper presented in 6th European Conference on Grain legumes. Lisbon, Portugal., 2007.
  26. Ana Rita Seabra, Philippe Thoquet, Julie Cullimore, Helena Carvalho. "Analysis of transgenic medicago truncatula overexpressing glutamine synthetase in root nodules: phenotypic characterization and transcript profiling.". Paper presented in Nitrogen 2007- International Symposium on Inorganic Nitrogen Assimilation. Lancaster University, UK., 2007.
  27. Ana Rita Seabra, Carlos Fernández-Tornero, Guy Schoehn, Pedro J. B. Pereira, Helena Carvalho. "Decameric organization of glutamine synthetase from medicago truncatula.". Paper presented in Nitrogen 2007- International Symposium on Inorganic Nitrogen Assimilation. Lancaster University, UK., 2007.
  28. Ana Rita Seabra, Helena Carvalho, Pedro Pereira. "Three-dimensional structure of an eukaryotic glutamine synthetase.". Paper presented in 15º Congresso Nacional de Bioquímica. Aveiro, Portugal., 2006.
  29. Ana Rita Seabra and Helena Carvalho. "Silencing plastid located glutamine synthetase through RNAi in root nodules of transgenic Medicago truncatula.". Paper presented in 15º Congresso Nacional de Bioquímica. Aveiro, Portugal., 2006.
  30. Ligia Lima, Ana Rita Seabra, Paula Melo, Julie Cullimore and Helena Carvalho. "Identification of serine 97 as a major regulatory phosphorylation site in plastid glutamine synthetase from Medicago truncatula.". Paper presented in IX Congresso Luso-Espanhol de Fisiologia Vegetal. Évora, Portugal, 2005.
  31. Lígia Lima, Ana Rita Seabra, Paula Melo, Julie Cullimore and Helena Carvalho. "14-3-3 binding controls proteolysis of plastid located glutamine synthetase in Medicago truncatula.". Paper presented in IX Congresso Luso-Espanhol de Fisiologia Vegetal. Évora, Portugal., 2005.
  32. Paula Melo, Lígia Lima, Isabel Santos, Julie Cullimore and Helena Carvalho. "Post-translational regulation of individual glutamine synthetase isoenzymes in the model legume Medicago truncatula by protein phosphorylation.". Paper presented in 13º Congresso Nacional de Bioquímica. Lisboa, Portugal., 2002.
  33. Paula Melo, Lígia Lima, Helena Carvalho and Julie Cullimore. "Studies on the regulation of plastid-located glutamine synthetase in root nodules of Medicago truncatula.". Paper presented in 6th International Symposium on Inorganic Nitrogen Assimilation. Reims, France., 2001.
  34. Lígia Lima, Paula Melo, Julie Cullimore and Helena Carvalho. "Studies on the phosphorylation of individual glutamine synthetase isoenzymes in the model legume Medicago truncatula.". Paper presented in 6th International Symposium on Inorganic Nitrogen Assimilation. Reims, France., 2001.
  35. Inês Lopes Cardoso, Helena Carvalho, Lígia Lima, Paula Melo and Julie Cullimo. "Does nodule specific modulation of glutamine synthetase (GS) in transgenic Medicago truncatula affect plant nitrogen metabolism?". Paper presented in 4th European Nitrogen Fixation Conference. Sevilha, Spain., 2000.
  36. Paula Melo, Inês Lopes Cardoso, Helena Carvalho and Julie Cullimore. "Expression of plastid-located glutamine synthetase in root nodules of Medicago truncatula.". Paper presented in 4th European Nitrogen Fixation Conference. Sevilha, Spain., 2000.
  37. Helena Carvalho, Lígia Lima, Roberto Salema and Julie Cullimore. "Differential expression of the two cytosolic glutamine synthetase genes in various organs of Medicago truncatula.". Paper presented in 12º Congresso Nacional de Bioquímica. Póvoa do Varzim, Portugal., 2000.
  38. Helena Carvalho, Lígia Lima, Roberto Salema and Julie Cullimore. "Immunogold localisation of glutamine synthetase in Medicago truncatula root nodules.". Paper presented in 12th International Congress on Nitrogen Fixation. Foz do Iguaçu, Brasil., 1999.
  39. Inês Lopes Cardoso, Helena Carvalho, Lígia Lima, Paula Melo, Roberto Salema and Julie Cullimore. "Nodule specific Modulation of Glutamine synthetase (GS) in transgenic Medicago truncatula.". Paper presented in 12th International Congress on Nitrogen Fixation. Foz do Iguaçu, Brasil., 1999.
  40. Helena Carvalho, Inês Cardoso, Nicole Lescure, Mireille Chabaud, Roberto Salema, and Julie Cullimore. "Localisation of the expression of cytosolic glutamine synthetase genes in Medicago truncatula.". Paper presented in 5th International Symposium on Inorganic Nitrogen Assimilation. Luso, Portugal., 1998.
  41. Helena Carvalho, Inês Lopes-Cardoso, Françoise de Billy, Mireille Chabaud, Nicole Lescure and Julie Cullimore. "Characterisation of the two cytosolic glutamine synthetase genes expressed in nodules of Medicago truncatula.". Paper presented in Third European Nitrogen Fixation Conference. Lunteren, Holanda, 1998.
  42. Helena Carvalho, Inês Cardoso, Roberto Salema, Nicole Lescure and Julie Cullimore. "Localisation and regulation of cytosolic glutamine synthetase in nodules of Medicago truncatula.". Paper presented in 11th International Congress on Nitrogen Fixation. Paris, France., 1997.
  43. Helena Carvalho, Roberto Salema and Julie Cullimore. "Complementation of a glnA- E.coli mutant with plant glutamine synthetase enzymes.". Paper presented in X Congresso Nacional de Bioquímica. Braga, Portugal., 1996.
  44. Susana Pereira, Helena Carvalho, Claudio Sunkel and Roberto Salema. "Glutamina Sintetase em Solanum tuberosum L: localização em folhas estioladas e após reverdecimento.". Paper presented in Jornadas de Estrutura, Desenvolvimento e Fisiologia Vegetal. Porto, Portugal., 1992.
  45. Helena Carvalho, Susana Pereira, Claudio Sunkel, Roberto Salema. "Distribution of Glutamine Synthetase isoforms in leaves of Solanum tuberosum L.". Paper presented in 8th Congress of the Federation of the European Societies of Plant Physiology (FESPP). Antuérpia, Holanda., 1992.
  46. Susana Pereira, Helena Carvalho, Claudio Sunkel, Roberto Salema. "Characterisation of Glutamine Synthetase from Solanum tuberosum L. leaves.". Paper presented in IV Portuguese-Spanish Biochemistry Congress. Póvoa de Varzim, Portugal., 1991.
  47. Helena Carvalho, Susana Pereira, Claudio Sunkel, Roberto Salema. "Immunocytochemical localisation of GS in green leaves and callus tissue of Solanum tuberosum L.". Paper presented in XXV Reunião Anual da Sociedade Portuguesa de Microscopia Electrónica. Porto, Portugal., 1990.
  48. Helena Carvalho, Susana Pereira, Claudio Sunkel, Roberto Salema. "Cell specific localisation of chloroplast and Cytosolic GS isoforms in leaves of Solanum tuberosum L.". Paper presented in Coloquio Franco-Ibérico de Microscopia Electrónica. Barcelona, Espanha., 1990.
Journal article
  1. Carvalho, Maria. "Characterization of plant glutamine synthetase S-nitrosation.". Nitric oxide : biology and chemistry (2019): https://doi.org/10.1016/j.niox.2019.04.006.
    10.1016/j.niox.2019.04.006
  2. Blanquet P; Silva L; Catrice O; Bruand C; Carvalho H; Meilhoc E. "Sinorhizobium meliloti controls NO-mediated post-translational modification of a Medicago truncatula nodule protein.". (2015): http://europepmc.org/abstract/med/26422404.
  3. Seabra AR; Carvalho HG. "Glutamine synthetase in Medicago truncatula, unveiling new secrets of a very old enzyme.". (2015): http://europepmc.org/abstract/med/26284094.
    10.3389/fpls.2015.00578
  4. Torreira E; Seabra AR; Marriott H; Zhou M; Llorca Ó; Robinson CV; Carvalho HG; Fernández-Tornero C; Pereira PJ. "The structures of cytosolic and plastid-located glutamine synthetases from Medicago truncatula reveal a common and dynamic architecture.". (2014): http://europepmc.org/abstract/med/24699643.
    10.1107/S1399004713034718
  5. Wang, Mingyi; Verdier, Jerome; Benedito, Vagner A.; Tang, Yuhong; Murray, Jeremy D.; Ge, Yinbing; Becker, Jörg D.; et al. "LegumeGRN: A Gene Regulatory Network Prediction Server for Functional and Comparative Studies". PLoS ONE 8 7 (2013): e67434. http://dx.doi.org/10.1371/journal.pone.0067434.
    10.1371/journal.pone.0067434
  6. Moreira, Sofia; Bishopp, Anthony; Carvalho, Helena; Campilho, Ana. "AHP6 Inhibits Cytokinin Signaling to Regulate the Orientation of Pericycle Cell Division during Lateral Root Initiation". Plos One 8 2 (2013):
    10.1371/journal.pone.0056370
  7. Seabra, A. R.; Silva, L. S.; Carvalho, H. G.. "Novel aspects of glutamine synthetase (GS) regulation revealed by a detailed expression analysis of the entire GS gene family of Medicago truncatula under different physiological conditions". BMC Plant Biol 13 1 (2013): 137-137.
    10.1186/1471-2229-13-137
  8. Silva,Liliana Santos; Carvalho,Helena G. "Possible role of glutamine synthetase in the NO signaling response in root nodules by contributing to the antioxidant defenses". Frontiers in Plant Science 4 (2013):
    10.3389/fpls.2013.00372
  9. Moreira, S.; Braga, T.; Carvalho, H.; Campilho, A.. "The Arabidopsis HP6 gene is expressed in Medicago truncatula lateral roots and root nodule primordia". Plant Signal Behav 8 8 (2013):
  10. Seabra, A. R.; Pereira, P. A.; Becker, J. D.; Carvalho, H. G.; Seabra, A.R.; Pereira, P.A.; Becker, J.D.; Carvalho, H.G.. "Inhibition of Glutamine Synthetase by Phosphinothricin Leads to Transcriptome Reprograming in Root Nodules of Medicago truncatula". Molecular Plant-Microbe Interactions 25 7 (2012): 976-992. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863301339&partnerID=MN8TOARS.
    10.1094/mpmi-12-11-0322
  11. Melo, Paula M.; Silva, Liliana S.; Ribeiro, Isa; Seabra, Ana R.; Carvalho, Helena G.. "Glutamine Synthetase Is a Molecular Target of Nitric Oxide in Root Nodules of Medicago truncatula and Is Regulated by Tyrosine Nitration". Plant Physiology 157 3 (2011): 1505-1517.
    10.1104/pp.111.186056
  12. Seabra, Ana R.; Vieira, Cristina P.; Cullimore, Julie V.; Carvalho, Helena G.. "Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds". Bmc Plant Biology 10 (2010):
    10.1186/1471-2229-10-183
  13. Seabra, Ana Rita; Carvalho, Helena; Barbosa Pereira, Pedro Jose. "Crystallization and preliminary crystallographic characterization of glutamine synthetase from Medicago truncatula". Acta Crystallographica Section F-Structural Biology and Crystallization Communications 65 (2009): 1309-1312.
    10.1107/S1744309109047381
  14. Barsch, Aiko; Carvalho, Helena G.; Cullimore, Julie V.; Niehaus, Karsten. "GC-MS based metabolite profiling implies three interdependent ways of ammonium assimilation in Medicago truncatula root nodules". Journal of Biotechnology 127 1 (2006): 79-83.
    10.1016/j.jbiotec.2006.06.007
  15. Lima, L; Seabra, A; Melo, P; Cullimore, J; Carvalho, H. "Phosphorylation and subsequent interaction with 14-3-3 proteins regulate plastid glutamine synthetase in Medicago truncatula". Planta 223 3 (2006): 558-567.
    10.1007/s00425-005-0097-8
  16. Lima, Ligia; Seabra, Ana; Melo, Paula; Cullimore, Julie; Carvalho, Helena. "Post-translational regulation of cytosolic glutamine synthetase of Medicago truncatula". Journal of Experimental Botany 57 11 (2006): 2751-2761.
    10.1093/jxb/erl036
  17. Carvalho, HG; Lopes-Cardoso, IA; Lima, LM; Melo, PM; Cullimore, JV. "Nodule-specific modulation of glutamine synthetase in transgenic Medicago truncatula leads to inverse alterations in asparagine synthetase expression". Plant Physiology 133 1 (2003): 243-252.
    10.1104/pp.102.017830
  18. Melo, PM; Lima, LM; Santos, IM; Carvalho, HG; Cullimore, JV. "Expression of the plastid-located glutamine synthetase of Medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids". Plant Physiology 132 1 (2003): 390-399.
    10.1104/pp.102.016675
  19. Carvalho, H; Lescure, N; de Billy, F; Chabaud, M; Lima, L; Salema, R; Cullimore, J. "Cellular expression and regulation of the Medicago truncatula cytosolic glutamine synthetase genes in root nodules". Plant Molecular Biology 42 5 (2000): 741-756.
    10.1023/A:1006304003770
  20. Carvalho, H; Lima, L; Lescure, N; Camut, S; Salema, R; Cullimore, J. "Differential expression of the two cytosolic glutamine synthetase genes in various organs of Medicago truncatula". Plant Science 159 2 (2000): 301-312.
    10.1016/S0168-9452(00)00360-5
  21. Carvalho, H; Sunkel, C; Salema, R; Cullimore, JV. "Heteromeric assembly of the cytosolic glutamine synthetase polypeptides of Medicago truncatula: complementation of a glnA Escherichia coli mutant with a plant domain-swapped enzyme". Plant Molecular Biology 35 5 (1997): 623-632.
    10.1023/A:1005884304303
  22. Carvalho, H.; Pereira, S.; Sunkel, C.; Salema, R.. "Detection of a cytosolic glutamine synthetase in leaves of Nicotiana tabacum L. by immunocytochemical methods". Plant Physiology 100 3 (1992): 1591-1594. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000267910&partnerID=MN8TOARS.
  23. Pereira, S.; Carvalho, H.; Sunkel, C.; Salema, R.. "Immunocytolocalization of glutamine synthetase in mesophyll and phloem of leaves of Solanum tuberosum L.". Protoplasma 167 1-2 (1992): 66-73. http://www.scopus.com/inward/record.url?eid=2-s2.0-0002812626&partnerID=MN8TOARS.
    10.1007/BF01353582