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Margarida Rasteiro. Completed the Mestrado in Biologia Molecular e Genética in 2017/10 by Universidade de Lisboa Faculdade de Ciências and Licenciatura in Biologia in 2015/07/28 by Universidade de Coimbra Faculdade de Ciencias e Tecnologia. Attends the Doutoramento in Mecanismos de Doença e Medicina Regenerativa (associação) by Universidade Nova de Lisboa Faculdade de Ciências Médicas since 2018/03.
Identification

Personal identification

Full name
MARGARIDA OLIVEIRA ALMEIDA RASTEIRO

Citation names

  • Rasteiro, Margarida

Author identifiers

Ciência ID
4D15-6D85-B973
ORCID iD
0000-0002-4891-4448

Email addresses

  • margarida.rasteiro@nms.unl.pt (Professional)
  • margarida.rasteiro@medicina.ulisboa.pt (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Biology
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2018/03 - 2022
Ongoing
 Mecanismos de Doença e Medicina Regenerativa (associação) (Doutoramento)
Major in Sem especialidade
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
"Gene therapy approaches to restore ciliary function in CRISPR-Cas9 zebrafish mutant models of Primary Ciliary Dyskinesia " (THESIS/DISSERTATION)
2015/09 - 2017/10
Concluded
 Biologia Molecular e Genética (Mestrado)
Major in Sem especialização
Universidade de Lisboa Faculdade de Ciências, Portugal
"Creation of a Danio rerio mutant using CRISPR-Cas9 as a model system to study Primary Ciliary Dyskinesia (PCD)" (THESIS/DISSERTATION)
 17/20
2012/09/10 - 2015/07/28
Concluded
 Biologia (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
 15
Projects

Grant

Designation Funders
2018/03/01 - 2022/09/30 Gene therapy approches to restore ciliary function in CRISPR-Cas9 zebrafish mutant models Primary Ciliary Dyskinesia
PD/BD/136927/2018
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2016/05/01 - 2019/10/31 The role of fluid dynamics in the molecular mechanisms of organ laterality.
PTDC/BEX-BID/1411/2014
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal

iNOVA4Health, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Journal article
  1. Pinto, Andreia L.; Rasteiro, Margarida; Bota, Catarina; Pestana, Sara; Sampaio, Pedro; Hogg, Claire; Burgoyne, Thomas; Lopes, Susana S.. "Zebrafish Motile Cilia as a Model for Primary Ciliary Dyskinesia". International Journal of Molecular Sciences 22 16 (2021): 8361. http://dx.doi.org/10.3390/ijms22168361.
    Published • 10.3390/ijms22168361
  2. Zietkiewicz, Ewa; Bukowy-Bieryllo, Zuzanna; Rabiasz, Alicja; Daca-Roszak, Patrycja; Wojda, Alina; Voelkel, Katarzyna; Rutkiewicz, Ewa; et al. "CFAP300: Mutations in Slavic Patients with Primary Ciliary Dyskinesia and a Role in Ciliary Dynein Arms Trafficking". American Journal of Respiratory Cell and Molecular Biology 61 4 (2019): 440-449. http://dx.doi.org/10.1165/rcmb.2018-0260oc.
    10.1165/rcmb.2018-0260oc
Thesis / Dissertation
  1. Rasteiro, Margarida Oliveira Almeida. "Creation of a Danio rerio mutant using CRISPR-Cas9 as a model system to study Primary Ciliary Dyskinesia (PCD)". Master, 2017. http://hdl.handle.net/10451/31518.
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/03/01 - 2022/03/31 Report project in cell and molecular biology
Supervisor
 Cellular and molecular biology (Scientific initiation)
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021/05/01 - 2021/05/31 Restoration of the ciliary function in the olfactory pit of a zebrafish mutant line using transient gene therapy
Co-supervisor
 Biochemistry (Scientific initiation)
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2020/03/01 - 2020/03/30 Using zebrafish mutant models of Primary Ciliary Dyskinesia to restore ciliary function
Co-supervisor
 Biochemistry (Scientific initiation)
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/10/01 - 2018/10/31 Analysis of the cilia-related phenotypes in CFAP300-knockdown zebrafish: Short Scientific Mission in Lisbon
Co-supervisor
Institute of Human Genetics, Poland

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2019/10/23 - 2019/10/29 European Developmental Biology Congress (with poster presentation)
Congress
 European Developmental Biology Congress
Spanish Society for Developmental Biology, Spain
2019/06/01 - 2019/06/30 Practical course on laboratory animal science in zebrafish and mice Covering all topics recommended by FELASA
Workshop
 Practical course on laborator y animal science in zebrafish and mice
Instituto Gulbenkian de Ciência, Portugal
2019/03/26 - 2019/03/29 4th BEAT-PCD Conference and 5th PCD Training School (with poster presentation)
Conference
 4th BEAT-PCD Conference and 5th PCD Training School
BEAT-PCD: Better experimental a approaches to treat PCD, Poland
2018/09/24 - 2018/09/28 IV Quantitative Fluorescence Microscopy Course -multiphoton microscopy -confocal microscopy -spinning disk confocal microscopy -deconvolution (Huygens) -image analysis (FIJI/ImageJ) -introduction to Imaris
Workshop
 IV Quantitative Fluorescence Microscopy Course
Center for Neuroscience and Cell Biology, Poland

Scientific expedition

Activity description Institution / Organization
2019/07/10 - 2019/07/26 Training in diagnosis of primary ciliary dyskinesia (PCD) - LYSOCIL Exchange Program University of Münster - Department of General Paediatrics, Germany