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Concluded, in 2018, is doctoral degree (PhD) in the Biochemistry area at Institute of Biosciences at the University of São Paulo, in Brazil. He got his master degree, in 2012, in Molecular Biotechnology at University of Essex, in England. And completed is graduation in Biochemistry at University of Beira Interior, in Portugal, in 2010. Currently works as scientific collaborator in the Laboratory of Proteins and Redox Biology in the Institute of Biosciences at the University of São Paulo, in Brazil. Research focus - Redox biochemistry, specifically studying some important enzymatic systems, such as: Organic Hydroperoxide Resistance proteins (Ohr); Thioredoxin/thioredoxin reductase system (Trx/TrxR); Peroxiredoxins. Principal Techniques: 1 - X-ray crystalography 2 - Protein engineering, cloning, expression and purification 3 - Differential scanning Fluorimetry (drug discovery) 4 - Pharmacophore model & Virtual Screening (drug discovery) 5 - MM and QM-MM dynamics
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Identification

Personal identification

Full name
Renato Mateus Domingos

Citation names

  • Domingos, Renato M.
  • Renato Mateus Domingos

Author identifiers

Ciência ID
4B15-04C8-872B
ORCID iD
0000-0002-6928-4836
Scopus Author Id
57193856926

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Upper intermediate (B2) Upper intermediate (B2) Elementary (A2) Upper intermediate (B2) Beginner (A1)
Education
Degree Classification
2020/01/10 - 2020/04/09
Ongoing
 StartUp Research (Pós-Graduação)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Universidade Nova de Lisboa Nova School of Business and Economics, Portugal
2014/02 - 2018/12
Concluded
 Biological Sciences (emphasis in Biochemistry) (Doutoramento)
Major in Biochemistry
Universidade de São Paulo Instituto de Biociências, Brazil
"Conserved Structural and Dynamic Aspects Behind Ohr Enzymatic Catalysis: Ohr as Potential Drug Targets" (THESIS/DISSERTATION)
2017/07 - 2017/08
Concluded
 Fundamentals in molecular simulations of biomolecules (Curso de Especialização Tecnológica)
Major in Molecular dynamics
Universidad de Buenos Aires Facultad de Ciencias Exactas y Naturales, Argentina
2016/04 - 2016/04
Concluded
 RapiData 2016: Data collection and Structure Solving (Curso de Especialização Tecnológica)
Major in Crystallography
Stanford Synchrotron Radiation Lightsource, United States
2016/04 - 2016/04
Concluded
 Crystallization: micro and nano crystals and high throughput methods (Curso de Especialização Tecnológica)
Major in Crystallography
Stanford Synchrotron Radiation Lightsource, United States
2016/03 - 2016/04
Concluded
 CCP4 - Macromolecular Crystallography School 2016 (Curso de Especialização Tecnológica)
Major in Crystallography
Universidade de São Paulo Instituto de Física de São Carlos, Brazil
2011/09 - 2012/10
Concluded
 Biotechnology (Mestrado)
Major in Molecular Biotechnology
University of Essex Department of Biological Sciences, United Kingdom
"Plant Root and Arbuscular Mycorrhizal’s Hyphal Network Density Effects on Soil Moisture Migration" (THESIS/DISSERTATION)
2006/09 - 2010/07
Concluded
 Biochemistry (Licenciatura)
Major in Biochemistry
Universidade da Beira Interior - Departamento de Química, Portugal
"Quantification of LSD in Biological fluids" (THESIS/DISSERTATION)
Affiliation

Science

Category
Host institution 		Employer
2019/05/01 - Current Postdoc (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014/02/01 - 2019/02/15 Researcher (Research) Universidade de São Paulo Instituto de Biociências, Brazil
2017/04 - 2017/09 Research Trainee (Research) Center for Structural Biology of MERCOSUR, Brazil
Universidad de Buenos Aires Facultad de Ciencias Exactas y Naturales, Argentina
(...)
2013/05 - 2014/01 Research Trainee (Research) Universidade de São Paulo Instituto de Biociências, Brazil
Laboratory of Proteins and Redox Biology - PI Luis Netto, Brazil
Projects

Contract

Designation Funders
2019/05/01 - Current Unravelling Geobacter sulfurreducens metabolism for bioelectricity production Project
PTDC/BIA-BQM/29118/2017 Principal
Post-doc Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Ongoing
2014/02 - 2018/03 Conserved structural and dynamic aspects behind Ohr enzymatic catalysis: Ohr as potential drug targets
CEPID Redoxoma - 2013/07937-8
PhD Student Fellow
Universidade de São Paulo Instituto de Biociências, Brazil

Universidad de Buenos Aires Facultad de Ciencias Exactas y Naturales, Argentina

Centro Nacional de Pesquisa em Energia e Materiais, Brazil
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Concluded

Other

Designation Funders
2017 - Current Functional characterization of the MarR family transcription factors from Chromobacterium violaceum
FAPESP
Researcher
Universidade de São Paulo Faculdade de Medicina de Ribeirão Preto, Brazil
Ongoing
2016 - Current Evaluation of the bactericide activity of a newly identified pyocin S8 against different strains of multi-resistant Pseudomonas aeruginosa
FAPESP
Researcher
Universidade de São Paulo Instituto de Biociências, Brazil
Ongoing
2015 - Current Unveiling the biological role of LsfA, a 1-Cys Prx involved in the virulence of Pseudomonas aeruginosa.
CEPID Redoxoma - 2013/07937-8
Researcher
Universidade de São Paulo Instituto de Biociências, Brazil
Ongoing
2014 - Current Phylogenetics Studies on Ohr/OsmC Protein Family
CEPID Redoxoma - 2013/07937-8
Researcher
Ongoing
2014 - Current Search for Inhibitor compounds for TrxR from Mycobacterium tuberculosis
FAPESP
Universidade de São Paulo Instituto de Química, Brazil

Universidade de São Paulo Instituto de Biociências, Brazil
Outputs

Publications

Conference abstract
  1. Silva, Rogério Luis Aleixo; Domingos, Renato M.; Luis Eduardo Soares Netto. "Structural and Biochemical Characterization of LsfA, a 1-Cys Prx involved in Pseudomonas aeruginosa virulence". Paper presented in SfRBM – Abstracts of 2019 SfRBM's 26th Annual Meeting held Las Vegas, NV, USA, Las Vegas, 2019.
    Published
  2. Domingos, Renato M.; Luis Eduardo Soares Netto. "Substrate-Assisted Catalysis: Molecular Aspects Behind Unique Structural Switches Along Ohr Catalytic Cycle". Paper presented in SfRBM – Abstracts of 2019 SfRBM's 26th Annual Meeting held Las Vegas, NV, USA, Las Vegas, 2019.
    Published
  3. Domingos, Renato M.; Teixeira, Raphael D.; Zeida, Ari; Agudelo, William A.; Alegria, Thiago G.P.; Murakami, Mario T.; Estrin, Dario A.; Netto, Luis E.S.. "Substrate triggered structural movements in Ohr: dihydrolipoamide accelerates the approximation of catalytic Arg towards the active site". Paper presented in 19th Society for Free Radical Research International (SFRRI) Biennial Meeting, Lisboa, 2018.
    Published • 10.1016/j.freeradbiomed.2018.04.308
  4. Gomes, Helena Turano; Gomes, Fernando; Domingos, Renato; Lincopan, Nilton; Gales, Ana Cristina; Netto, Luis. "Structural characterization of a newly identified pyocin S8, a proteic antibiotic from Pseudomonas aeruginosa with potential therapeutic applications". Paper presented in 2018 SfRBM's 25th Annual Meeting, Chicago, 2018.
    Published • 10.1016/j.freeradbiomed.2018.10.271
  5. Henrique Yokomizo, Cesar; Geronimo Alegria, Thiago; Meireles, Diogo de Abreu; Weissmann Gaiarsa, Jonas; Gomes Ragnoni, Eduardo; Mateus Domingos, Renato; Netto, Luis Eduardo Soares. "Studies of Disulfide Reductase Activity and Phylogenetic Distribution of Xylella Fastidiosa YbbN Protein". Paper presented in 23rd Annual Meeting of Society for Redox Biology and Medicine, a joint meeting with the Society for Free Radical Research International (SfRBM||SFRRI), Chicago, 2016.
    Published • 10.1016/j.freeradbiomed.2016.10.299
  6. Meireles, Diogo de Abreu; Mateus Domingos, Renato; Alegria, Thiago G; Netto, Luis Eduardo Soares. "Oral Presentation - Analyses of Amino Acid Sequences and Tertiary Structures among Ohr Enzymes Revealed a Catalytic Role for Y126". Paper presented in 23rd Annual Meeting of Society for Redox Biology and Medicine, a joint meeting with the Society for Free Radical Research International (SfRBM||SFRRI), 2016.
    Published • 10.1016/j.freeradbiomed.2016.10.067
  7. Mateus Domingos, Renato; Abreu Meireles, Diogo; Freire Neto, Jose; Geronimo Alegria, Thiago; Tyago Murakami, Mdrio; Dias Teixeira, Raphael; Eduardo Netto, Luis. "Structural and Biochemical Analysis of the Open Conformation of OhrA and OhrB from Chromobacterium Violaceum, Two Cys-Based Proxidases with Extraordinary Reactivity towards Organic Peroxides". Paper presented in 22nd Annual Meeting of Society for Redox Biology and Medicine (SFRBM), Boston, 2015.
    Published • 10.1016/j.freeradbiomed.2015.10.377
  8. de Abreu Meireles, Diogo; Mateus Domingos, Renato; Weissmann Gaiarsa, Jonas; de Souza, Robson Francisco; Soares Netto, Luis Eduardo. "Functional and Biochemical Characterization of the First Ohr Peroxidase Identified in Eukaryotes". Paper presented in 22nd Annual Meeting of Society for Redox Biology and Medicine (SFRBM), Boston, 2015.
    Published • 10.1016/j.freeradbiomed.2015.10.387
  9. Mateus Domingos, Renato; de Abreu Meireles, Diogo; da Silva Neto, José Freire; Gasparello Viviani, Lucas; Pires Alegria, Thiago; do Amaral, Antonia Tavares; Soares Netto, Luis. "Structural and Biochemical Comparative Analysis among Cys-Based Ohr/OsmC Protein Family: Insights on Their High Reactivity Towards Hydroperoxides". Paper presented in 21st Annual Meeting of Society for Redox Biology and Medicine (SFRBM), Seattle, 2014.
    Published • 10.1016/j.freeradbiomed.2014.10.061
  10. de Abreu Meireles, Diogo; Mateus Domingos, Renato; de Souza, Robson Francisco; Soares Netto, Luis Eduardo. "Distribution and Classification of Cys-Based Proteins of the Ohr/OsmC Family". Paper presented in 21st Annual Meeting of Society for Redox Biology and Medicine (SFRBM), Seattle, 2014.
    Published • 10.1016/j.freeradbiomed.2014.10.118
Conference poster
  1. Domingos, Renato M.; Catarina M Paquete; Américo G Duarte. "Involvement of Geobacter sulfurreducens ImcH and CbcL in extracellular electron transfer for bioelectricity production". Paper presented in Microbiotech 19, 2019.
  2. Domingos, Renato M.; Dias Teixeira, Raphael; Zeida, Ari; Agudelo, William A.; Alegria, Thiago G. P.; Murakami, Mario T.; Estrin, Dario A.; Netto, L.E.S.. "The unique reducing step of organic hydroperoxide resistance proteins: is the catalytic arginine important to activate the reducing agent?". Paper presented in 42nd Brazilian Society for Biophysics (SBBf), 2017.
  3. Domingos, Renato M.; Dias Teixeira, Raphael; Zeida, Ari; Alegria, Thiago G. P.; Estrin, Dario A.; Netto, L.E.S.. "First Crystallographic Structure of the Interaction Between an Organic Hydroperoxide Resistance Protein and its Biological Reductant: Structural and Molecular Dynamic Analyses". Paper presented in 46th Brazilian Society for Biochemistry and Molecular Biology (SBBq), 2017.
  4. Domingos, Renato M.; de Abreu Meireles, Diogo; Dias Teixeira, Raphael; Alegria, Thiago G. P.; Netto, L.E.S.. "Dimerization of Organic Hydroperoxide Resistance Proteins is Required for Proper Architecture of Amino Acids in the Active Site: Analysis of a High-Resolution Structure of OhrA from Chromobacterium violaceum". Paper presented in V Latin American Protein Society Meeting (LAPSM), 2016.
  5. Domingos, Renato M.; Meireles, Diogo de Abreu; da Silva Neto, José Freire; Alegria, Thiago G. P.; Dias Teixeira, Raphael; Netto, L.E.S.. "The First Structural and Biochemical Comparative analysis of two Organic Hydroperoxide Resistance Paralogues: The Chromobacterium violaceum OhrA and OhrB". Paper presented in 23rd International Union of biochemistry and Molecular Biology (IUBMB) || 44th Brazilian Society for Biochemistry and Molecular Biology (SBBq), 2015.
  6. Abreu Meireles, Diogo; Domingos, Renato M.; Gaiarsa, J.W.; Alegria, Thiago G. P.; de Souza, R.F.; Netto, L.E.S.. "Use of residue conservation analysis to identify functional and structural conserved residues of Ohr/OsmC family". Paper presented in 23rd International Union of biochemistry and Molecular Biology (IUBMB) || 44th Brazilian Society for Biochemistry and Molecular Biology (SBBq), 2015.
Journal article
  1. Turano, Helena; Gomes, Fernando; Domingos, Renato M.; Degenhardt, Maximilia F. S.; Oliveira, Cristiano L. P.; Garratt, Richard C.; Lincopan, Nilton; Netto, Luis E. S.. "Molecular structure and functional analysis of pyocin S8 from Pseudomonas aeruginosa reveals the essential requirement of a glutamate residue in the H-N-H motif for DNase activity". Journal of Bacteriology (2020): http://dx.doi.org/10.1128/jb.00346-20.
    Published • 10.1128/jb.00346-20
  2. Mateus Domingos, Renato; Teixeira, Raphael D.; Zeida, Ari; Agudelo, William A.; Alegria, Thiago G.P.; da Silva Neto, José F; Vieira, Plinio S.; et al. "Substrate and product-assisted catalysis: molecular aspects behind structural switches along Organic Hydroperoxide Resistance Protein catalytic cycle". ACS Catalysis (2020): http://dx.doi.org/10.1021/acscatal.0c01257.
    Accepted • 10.1021/acscatal.0c01257
  3. Oliveira, Ana Rita; Mota, Cristiano; Mourato, Cláudia; Domingos, Renato M.; Santos, Marino F. A.; Gesto, Diana; Guigliarelli, Bruno; et al. "Toward the mechanistic understanding of enzymatic CO2 reduction". ACS Catalysis (2020): http://dx.doi.org/10.1021/acscatal.0c00086.
    10.1021/acscatal.0c00086
  4. Piccirillo, Erika; Alegria, Thiago G. P.; Discola, Karen F.; Cussiol, José R. R.; Domingos, Renato M.; de Oliveira, Marcos A.; Rezende, Leandro de; Netto, Luis E. S.; Amaral, Antonia T-do. "Structural insights on the efficient catalysis of hydroperoxide reduction by Ohr: Crystallographic and molecular dynamics approaches". PLOS ONE 13 5 (2018): e0196918. http://dx.doi.org/10.1371/journal.pone.0196918.
    10.1371/journal.pone.0196918
  5. Meireles, D.A.; Domingos, R.M.; Gaiarsa, J.W.; Ragnoni, E.G.; Bannitz-Fernandes, R.; da Silva Neto, J.F.; de Souza, R.F.; Netto, L.E.S.. "Functional and evolutionary characterization of Ohr proteins in eukaryotes reveals many active homologs among pathogenic fungi". Redox Biology 12 (2017): 600-609. http://www.scopus.com/inward/record.url?eid=2-s2.0-85017155790&partnerID=MN8TOARS.
    10.1016/j.redox.2017.03.026
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2016/12 Dimerization of Organic Hydroperoxide Resistance Proteins is Required for Proper Architecture of Amino Acids in the Active Site: Analysis of a High-Resolution Structure of OhrA from Chromobacterium violaceum V Latin American Protein Society Meeting (LAPSM)
Latin American Protein Society (Rio de Janeiro, Brazil)
2014 Comparative Research on Ohr/OsmC Protein Family as Potential Drug Targets I Workshop of Redox Signaling and Oxidative Stress Response,
Instituto de Biociencias da Universidade de Sao Paulo (Sao Paulo, Brazil)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2016 - 2018 Structural and biochemical characterization of LsfA, a 1-Cys Prx involved in virulence in Pseudomonas aeruginosa
Co-supervisor of Rogério Luis Aleixo Silva
 Genética e Biologia Evolutiva (Master)
Universidade de São Paulo Instituto de Biociências, Brazil

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2016/07 - 2016/12 Proteins, structure, function and cellular biology - BIO0452 : Program of teaching improvement Supervision: Prof. Dr Luis Eduardo Soares Netto Genetics and Evolutionary Biology (Bachelor) Universidade de São Paulo Instituto de Biociências, Brazil
2015/02 - 2015/07 Fundamentals of Molecular Biology - BIO205: Program of teaching improvement Supervision: Prof Dra Regina Celia Mingroni Neto Genetics and Evolutionary Biology (Bachelor) Universidade de São Paulo Instituto de Biociências, Brazil
Distinctions

Award

2017 SBBq Award 2017 - Best Poster
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil
2016 Young Scientist Travel Award
International Union of Crystallography, United Kingdom
2016 Young Scientist Travel Award
International Union of Crystallography, United Kingdom
2015 SBBq Award 2015 - Best Poster
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil

Other distinction

2016 Honorable Mention - PhD Research Project
Universidade de São Paulo Instituto de Biociências, Brazil