Luis Miguel Afonso Ramos de Carvalho (4410-D3B9-BA75)

Curriculum Vitae

Luis Miguel Afonso Ramos de Carvalho

Luis Carvalho completed the Master's Degree in Pharmaceutical Sciencies in 2013 in the Faculty of Pharmacy of the University of Lisbon. During this period, Luis received a research initiation grant from the University of Lisbon and Fundação Amadeu Dias in 2010. After this grant, Luis enroled in Rui Moreira's lab in 2011 as an undergraduate student to contact with Organic Chemistry Synthesis and develop enzymatic inhibitors for Human Neutrophil Elastase. Luis enrolled in an FCT-funded PhD with Rui Moreira's lab in the Medicinal Chemistry group of the Research Institute for Medicines of the University of Lisbon, in collaboration with Instituto Nacional de Saúde Dr. Ricardo Jorge, in the field of Medicinal Chemistry, working in the derivatization of small molecule enzymatic inhibitors as probes for diagnostic purposes. During his PhD, Luis received a Fulbright grant to work at the Cravatt Lab in The Scripps Research Institute in California, where he was trained in Activity-based Protein Profiling and Proteomics. During his PhD Luis co-organized two editions of the Postgraduate iMed.ULisboa Students Meeting, participated as a representative of iMed.ULisboa in the European Researcher's Night and worked as a volunteer with Scripps Assists of The Scripps Research Institute in the Lagoon Project, which aimed at the maintenance of the Torrey Pines Natural Reserve and local event organization. Luis completed his Phd in 2020 and readily enrolled in Post-doctoral research with the Bernardes Group at Instituto de Medicina Molecular, where he developed serine hydrolase targeting PROTACs and innovative proteomics platforms that took advantage of the extensive repertoire of the group in covalent chemical binders. In 2021 Luis received a Marie Curie Individual Fellowship to join the Bernardes Lab in the Yusuf Hamied Department of Chemistry in the University of Cambridge. Luis is currently a Senior Laboratory Research Scientist at The Francis Crick Institute, working in the application of proteomics technologies in the discovery and study of molecular glues.

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Identification

Personal identification

Full name: Luis Miguel Afonso Ramos de Carvalho

Citation names

Luis A. R. Carvalho

Author identifiers

Ciência ID: 4410-D3B9-BA75
ORCID iD: 0000-0001-5257-7301

Addresses

The Francis Crick Institute, 1 Midland Road, NW1 1AT, London, London, United Kingdom (Professional)

Websites

https://www.crick.ac.uk/research/platforms-and-facilities/proteomics (Professional)

Knowledge fields

Medical and Health Sciences - Basic Medicine - Medicinal Chemistry
Medical and Health Sciences - Basic Medicine - Medicinal Chemistry
Exact Sciences - Chemical Sciences - Organic Chemistry
Medical and Health Sciences - Other Medical Sciences

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
Portuguese (Mother tongue)

Education

	Degree	Classification
2021/10/01 - 2023/09/30 Ongoing	Marie Curie Postdoctoral Researcher (Pós-doutoramento) University of Cambridge Department of Chemistry, United Kingdom
2020/04/06 - 2021/09/30 Concluded	Post-doctoral Researcher (Pós-doutoramento) Instituto de Medicina Molecular João Lobo Antunes, Portugal
2015/03/01 - 2020/02/10 Concluded	Doutoramento em Farmácia - Especialidade Química Farmacêutica e Terapêutica (Doutoramento) Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal "3-Oxo-ß-Sultams and 4-Oxo-ß-Lactams as Chemical Tools for Activity-based Protein Profiling of Serine Hydrolases" (THESIS/DISSERTATION)	Aprovado com Louvor e Distinção
2013 Concluded	Mestrado Integrado em Ciências Farmacêuticas (Mestrado) Universidade de Lisboa Faculdade de Farmácia, Portugal "Monografia: Activity-Based Probes for the study of Proteases" (THESIS/DISSERTATION)	17 Valores

Affiliation

Science

	Category Host institution	Employer
2024/06/17 - Current	Contracted Researcher (Research)	The Francis Crick Institute, United Kingdom
	The Francis Crick Institute, United Kingdom
2021/10/01 - Current	Postdoc (Research)	University of Cambridge Department of Chemistry, United Kingdom
	University of Cambridge Department of Chemistry, United Kingdom
2020/04/06 - 2021/09/30	Postdoc (Research)	Instituto de Medicina Molecular João Lobo Antunes, Portugal
	Instituto de Medicina Molecular João Lobo Antunes, Portugal
2017/09/13 - 2018/07/09	Visiting Researcher (Research)	Scripps Research Institute, United States
	Scripps Research Institute, United States (...)

Others

	Category Host institution	Employer
2015/03/01 - 2020/02/10	PhD Student	Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal

2014/06/17 - 2014/10/17	Scientific Research in the Toxicology field. (MERTOX-TRX) Molecular Mechanisms of Mercury Toxicicty: Study of the inhibition of the thioredoxin system in the human population. (PTDC/QUI-BIQ/117281)	Universidade de Lisboa Faculdade de Farmácia, Portugal

2013/09/02 - 2013/10/31	Internship in Hospital Pharmacy, part of Pharmaceutical Sciences Master's Degree	Hospital CUF Descobertas, Portugal

2013/03/01 - 2013/06/28	Internship in Community Pharmacy, part of Pharmaceutical Sciences Master's Degree	Farmácia Comunitária do Hospital Santa Maria, Portugal

2010/12/23 - 2012/12/22	Monitor in supervision of Master's Degree classes: Organic Chemistry and Chemistry Physics. Contract with the Faculty of Pharmacy of the University of Lisbon.	Universidade de Lisboa Faculdade de Farmácia, Portugal

2010/12/23 - 2012/11/30	Scientific Research in Medicinal Chemistry - Rui Moreira's Grouop - Synthesis of Elastase Inhibitors	Universidade de Lisboa Faculdade de Farmácia, Portugal

2010/09/30 - 2011/10/12	Universidade de Lisboa - Fundação Amadeu Dias Grant 2010/2011 Project: "Analysis of the Release Profile of Levofloxacin from Calcium Phosphate Microparticles".	Universidade de Lisboa, Portugal

Projects

Grant

	Designation	Funders
2015/03/01 - 2020/02/10	ActiProbe: Actvity-based Probes as Chemical Tools for Biomarker Discovery in Chronic Obstructive Pulmonary Disease SFRH/BD/100400/2014 PhD Student Fellow Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2024/06/14 - Current	UKRI Prosperity Partnership - Rule book for molecular glue degraders EP/X025357/1 Researcher The Francis Crick Institute, United Kingdom	Engineering and Physical Sciences Research Council AstraZeneca Diagnostics Ongoing
2021/10/01 - 2023/09/30	ABPP-PROTDRUG - An Activity-based Protein An Activity-based Protein Profiling-supported Protein Therapeutic Drug development platform for the development of innovative anti-cancer therapies based on Lin28 inhibition 101022421 Post-doc University of Cambridge Department of Chemistry, United Kingdom	Horizon 2020 Ongoing
2020/04/06 - 2021/09/30	Intracellular Drug Delivery using Small Molecule Drug Conjugates for Prostate Cancer Therapy IMM/CT/3-2020 Post-doc Fellow Instituto de Medicina Molecular João Lobo Antunes, Portugal	Concluded

Other

	Designation	Funders
2015/03/01 - 2019/03/15	ProbeCOPD. Protease activity-based probes for Chronic Obstructive Pulmonary Disease diagnostics PTDC/BBB-BEP/2463/2014 PhD Student Fellow Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal	Concluded
2014/07 - 2014/10	(MERTOX-TRX) Molecular Mechanisms of Mercury Toxicity: Study of the inhibition of human thioredoxin system info:eu-repo/grantAgreement/FCT/3599-PPCDT/117281/PT Master Student Fellow Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal	Concluded

Outputs

Publications

Conference poster	Luis A. R. Carvalho. "Rational Design of PROTAC Linkers using the PRosettaC Software". Paper presented in SIMICA Summer School - Therapeutic Site-selective Protein-modification Chemistries, 2022. Luis A. R. Carvalho; K. M. Lum; B. Ross; S. D. Lucas; R. Huber; B. F. Cravatt; R. Moreira. "Activity-based Protein Profiling using 4-Oxo-ß-Lactams uncovers a new Chemotype for DPP8 and DPP9 Inhibition". Paper presented in 11th iMed.ULisboa Postgraduate Students Meeting & 4nd i3DU Meeting, 2019. Luis A. R. Carvalho; L. M. Gonçalves; F. Vaz; S. D. Lucas; D. Penque; R. Moreira. "A Chemoproteomic approach to validate Human Neutrophil Elastase as a Biomarker of Chronic Obstructive Pulmonary Disease". Paper presented in 1º Encontro – A Química na Investigação da ULisboa, 2017. Luis A. R. Carvalho; L. M. Gonçalves; F. Vaz; S. D. Lucas; D. Penque; R. Moreira. "A Chemoproteomic approach to validate Human Neutrophil Elastase as a Biomarker of Chronic Obstructive Pulmonary Disease". Paper presented in XXV Encontro Nacional da SPQ, 2017. Luis A. R. Carvalho; L. M. Gonçalves; F. Vaz; S. D. Lucas; D. Penque; R. Moreira. "A Chemoproteomic approach to validate Human Neutrophil Elastase as a Biomarker of Chronic Obstructive Pulmonary Disease". Paper presented in 9th iMed.ULisboa Postgraduate Students Meeting & 2nd i3DU Meeting, 2017. Luis A. R. Carvalho; R. Moreira; S. D. Lucas. "ACTIPROBE: Activity-based probes as chemical tools for biomarker discovery in chronic obstructive pulmonary disease". Paper presented in 5th Portuguese Young Chemists Meeting, 1st European Young Chemists Meeting, 2016. Luis A. R. Carvalho; R. Moreira; S. D. Lucas. "ACTIPROBE: Activity-based probes as chemical tools for biomarker discovery in chronic obstructive pulmonary disease". Paper presented in 11th National Encounter of Organic Chemistry, 4º National Encounter of Therapeutic Chemistry, 2015.
Journal article	Carvalho, Luís A. R.; Sousa, Bárbara B.; Zaidman, Daniel; Kiely-Collins, Hannah; Bernardes, Gonçalo J. L.. "Design and Evaluation of PROTACs Targeting Acyl Protein Thioesterase 1". ChemBioChem 25 4 (2024): https://doi.org/10.1002/cbic.202300736. 10.1002/cbic.202300736 Branco, Vasco; Carvalho, Luís; Barboza, Cássia; Mendes, Eduarda; Cavaco, Afonso; Carvalho, Cristina. "Selenium and Redox Enzyme Activity in Pregnant Women Exposed to Methylmercury". Antioxidants 11 11 (2022): 2291. http://dx.doi.org/10.3390/antiox11112291. Open access • Published • 10.3390/antiox11112291 Sousa, Bárbara B.; de Almeida, Cátia Rebelo; Barahona, Ana F.; Lopes, Raquel; Martins-Logrado, Ana; Cavaco, Marco; Neves, Vera; et al. "Selective Inhibition of Bruton's Tyrosine Kinase by a Designed Covalent Ligand Leads to Potent Therapeutic Efficacy in Blood Cancers Relative to Clinically Used Inhibitors". ACS Pharmacology & Translational Science 5 11 (2022): 1156-1168. http://dx.doi.org/10.1021/acsptsci.2c00163. 10.1021/acsptsci.2c00163 Carvalho, Luís A. R.; Ross, Breyan; Fehr, Lorenz; Bolgi, Oguz; Wöhrle, Svenja; Lum, Kenneth M.; Podlesainski, David; et al. "Chemoproteomics-Enabled Identification of 4-Oxo-ß-Lactams as Inhibitors of Dipeptidyl Peptidases 8 and 9". Angewandte Chemie International Edition 61 47 (2022): http://dx.doi.org/10.1002/anie.202210498. Open access • Published • 10.1002/anie.202210498 Carvalho, Luís A. R.; Ross, Breyan; Fehr, Lorenz; Bolgi, Oguz; Wöhrle, Svenja; Lum, Kenneth M.; Podlesainski, David; et al. "Chemoproteomik-basierte Identifikation von 4-Oxo-ß-lactamen als Inhibitoren der Dipeptidylpeptidasen 8¿und 9". Angewandte Chemie 134 47 (2022): http://dx.doi.org/10.1002/ange.202210498. Open access • Published • 10.1002/ange.202210498 Carvalho, Luís A. R.; Bernardes, Gonçalo J. L.. "The Impact of Activity-Based Protein Profiling in Malaria Drug Discovery". ChemMedChem 17 14 (2022): http://dx.doi.org/10.1002/cmdc.202200174. Open access • Published • 10.1002/cmdc.202200174 Barbosa, Marta; Gomes, Cátia; Sequeira, Catarina; Gonçalves-Ribeiro, Joana; Pina, Carolina Campos; Carvalho, Luís A.; Moreira, Rui; et al. "Recovery of Depleted miR-146a in ALS Cortical Astrocytes Reverts Cell Aberrancies and Prevents Paracrine Pathogenicity on Microglia and Motor Neurons". Frontiers in Cell and Developmental Biology 9 (2021): http://dx.doi.org/10.3389/fcell.2021.634355. 10.3389/fcell.2021.634355 Carvalho, Luís A. R.; Almeida, Vanessa T.; Brito, José A.; Lum, Kenneth M.; Oliveira, Tânia F.; Guedes, Rita C.; Gonçalves, Lídia M.; et al. "3-Oxo-ß-sultam as a Sulfonylating Chemotype for Inhibition of Serine Hydrolases and Activity-Based Protein Profiling". ACS Chemical Biology 15 4 (2020): 878-883. http://dx.doi.org/10.1021/acschembio.0c00090. Published • 10.1021/acschembio.0c00090 Vaz, Ana Rita; Pinto, Sara; Ezequiel, Catarina; Cunha, Carolina; Carvalho, Luís A.; Moreira, Rui; Brites, Dora. "Phenotypic Effects of Wild-Type and Mutant SOD1 Expression in N9 Murine Microglia at Steady State, Inflammatory and Immunomodulatory Conditions". Frontiers in Cellular Neuroscience 13 (2019): http://dx.doi.org/10.3389/fncel.2019.00109. 10.3389/fncel.2019.00109 Falcão, Ana S.; Carvalho, Luís A. R.; Lidónio, Gonçalo; Vaz, Ana R.; Lucas, Susana D.; Moreira, Rui; Brites, Dora. "Dipeptidyl Vinyl Sulfone as a Novel Chemical Tool to Inhibit HMGB1/NLRP3-Inflammasome and Inflamma-miRs in Aß-Mediated Microglial Inflammation". ACS Chemical Neuroscience 8 1 (2016): 89-99. http://dx.doi.org/10.1021/acschemneuro.6b00250. 10.1021/acschemneuro.6b00250 Ruivo, Eduardo F. P.; Gonçalves, Lídia M.; Carvalho, Luís A. R.; Guedes, Rita C.; Hofbauer, Stefan; Brito, José A.; Archer, Margarida; Moreira, Rui; Lucas, Susana D.. "Clickable 4-Oxo-ß-lactam-Based Selective Probing for Human Neutrophil Elastase Related Proteomes". ChemMedChem 11 18 (2016): 2037-2042. http://dx.doi.org/10.1002/cmdc.201600258. 10.1002/cmdc.201600258 Carvalho, L. A. R.; Ruivo, E. F. P.; Lucas, S. D.; Moreira, R.. "Activity-based probes as molecular tools for biomarker discovery". MedChemComm 6 4 (2015): 536-546. http://dx.doi.org/10.1039/c4md00417e. 10.1039/c4md00417e Lucas, Susana D.; Gonçalves, Lídia M.; Carvalho, Luís A. R.; Correia, Henrique F.; Da Costa, Eduardo M. R.; Guedes, Romina A.; Moreira, Rui; Guedes, Rita C.. "Optimization of O3-Acyl Kojic Acid Derivatives as Potent and Selective Human Neutrophil Elastase Inhibitors". Journal of Medicinal Chemistry 56 23 (2013): 9802-9806. http://dx.doi.org/10.1021/jm4011725. 10.1021/jm4011725
Thesis / Dissertation	Luis A. R. Carvalho. "3-Oxo-ß-Sultams and 4-Oxo-ß-Lactams as Chemical Tools for Activity-based Protein Profiling of Serine Hydrolases". PhD, Universidade de Lisboa Instituto de Investigação do Medicamento, 2020.

Other

Other output

Cover Picture: Chemoproteomics-Enabled Identification of 4-Oxo-ß-Lactams as Inhibitors of Dipeptidyl Peptidases 8 and 9 (Angew. Chem. Int. Ed. 47/2022). Front cover picture of Angewandte Chemie (Angew. Chem. Int. Ed. 47/2022). Designed by Luis A. R. Carvalho (Self). 2022. Luis A. R. Carvalho. https://onlinelibrary.wiley.com/doi/full/10.1002/anie.202213804.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2025/01/13	MS Imaging and Spatial Proteomics	3rd ISOBIOTICS Training Event GIMM (Lisbon, Portugal)
2022/09/01	Proteomics Solutions in Chemical Biology: 4-Oxo-ß-Lactams as Chemical Tools for Activity-based Protein Profiling of Serine Hydrolases & Rational Design of PROTAC Linkers	2nd Young Investigator Workshop 2022 EuChemS - Division of Chemistry in Life Sciences (Lisbon, Portugal)
2021/10/13	Serine Hydrolase PROTACs & Proteomics Solutions in Chemical Biology	Chemical Biology of Carbon Monoxide Symposium ACORN Project - A Nanoparticle-based Therapeutic Applications and Detection of Carbon Monoxide Releasing Molecules (Algarve, Portugal)
2019/03/29	Activity-based Protein Profiling of Serine Hydrolases using 4-Oxo-ß-Lactams	ABPP Symposium 2019 – Target Discovery and Visualization using Chemical Probes University of Leuven (Leuven, Belgium)
2017/07/06	Activity-Based Probes as Chemical Tools for Biomarker Discovery in Chronic Obstructive Pulmonary Disease	European School of Medicinal Chemistry ESMEC University of Urbino (Urbino, Italy)
2017/05/19	Activity-Based Probes as Chemical Tools for Biomarker Discovery in Chronic Obstructive Pulmonary Disease	IV SEQT Symposium of Medicinal Chemistry Young Researchers University of Barcelona (Barcelona, Spain)
2017/05/08	Activity-Based Probes as Chemical Tools for Biomarker Discovery in Chronic Obstructive Pulmonary Disease by a Proteomics-Based Approach	Young Investigator Day at Instituto Nacional de Saúde Dr. Ricardo Jorge Instituto Nacional de Saúde Doutor Ricardo Jorge (Lisbon, Portugal)
2015/07/15	Acti-Probe: Activity-based probes as chemical tools for biomarker discovery in Chronic Obstructive Pulmonary Disease	7th iMed.ULisboa Postgraduate Students Meeting Research Institute for Medicines (iMed.ULisboa) (Lisbon, Portugal)
2015/04/09	Blood and Plasma concentrations of Selenium in the Portuguese Population	Selenium and Selenoproteins in Health and Disease Research Institute for Medicines (iMed.ULisboa)

Event organisation

	Event name Type of event (Role)	Institution / Organization
2016 - 2017	Member of the organizing committee (ipSC – Postgraduate Students’ Association) of the 8th edition of the Postgraduate iMed.ULisboa Students Meeting (2016) Meeting (Member of the Organising Committee)	Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2015 - 2016	Member of the organizing committee (ipSC – Postgraduate Students’ Association) of the 7th edition of the Postgraduate iMed.ULisboa Students Meeting (2015) Meeting (Member of the Organising Committee)	Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2015 - Current	European Researcher’s Night – As a member of the ipSC of iMed.ULisboa, organization and participation in the activity Gelify yourself! with the preparation of carbopol gels, showcasing pharmaceutical formula development to the public.	European Researcher’s Night
2020/10/19 - 2020/10/22	Summer School and Thematic Workshop Meeting	Acorn - Nanoparticle-Based Therapeutic Applications and Detection of Carbon Monoxide Releasing Molecules - Summer School and Thematic Workshop Instituto de Medicina Molecular João Lobo Antunes, Portugal
2017/09 - 2018/07	Volunteer with Scripps Assists from The Scripps Research Institute at the Lagoon Project in San Diego, California. Activities included maintenance of the Torrey Pines Natural Reserve and local event organization at the reserve as part of the participation in the Fulbright program. Other	Scripps Research Institute, United States

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2022/05/31 - Current	Activity-based Protein Profiling (ABPP) 3-Oxo-ß-Sultams and 4-Oxo-ß-Lactams as Chemical Tools for Activity-based Protein Profiling of Serine Hydrolases - Invited Lecturer	Medicinal and Biopharmaceutical Chemistry (Mestrado)	Universidade de Lisboa Faculdade de Farmácia, Portugal

Distinctions

Award

2015	Winner of the 'Why Medicinal Chemistry?' Videoclip Competition The European Federation for Medicinal Chemistry (EFMC), Belgium
2015	Best Oral Presentation by a Young Investigator at the conference Selenium and Selenoproteins in Health and Disease sponsored by Santa Cruz Biotechnology. Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal

Other distinction

2022	Article 'Chemoproteomics-Enabled Identification of 4-Oxo-ß-Lactams as Inhibitors of Dipeptidyl Peptidases 8 and 9' highlighted as a 'Hot Paper' in Angewandte Chemie Volume 61, Issue 47
2022	Article 'Chemoproteomics-Enabled Identification of 4-Oxo-ß-Lactams as Inhibitors of Dipeptidyl Peptidases 8 and 9' featured on the main cover of Angewandte Chemie Volume 61, Issue 47.
2020	Marie Curie Individual Fellowship, H2020-MSCA-IF-2020 call for research in The University of Cambridge, UK. Horizon 2020, Belgium
2017	FCT-supported Fulbright Grant Fulbright Portugal, Portugal
2017	Grant to attend the European School of Medicinal Chemistry ESMEC in Urbino, July 2nd-6th, 2017. The European Federation for Medicinal Chemistry (EFMC), Belgium
2015	Best Oral Presentation by a Young Investigator at the conference 'Selenium and Selenoproteins in Health and Disease'.
2014	JmedChem article 'Optimization of O3-acyl kojic acid derivatives as potent and selective human neutrophil elastase inhibitors' featured by Global Medical Discovery as key drug discovery article.
2013	Prémio Escolar de Incentivo à Formação Pós-Graduada da Faculdade de Farmácia da Universidade de Lisboa, 3º Prémio - Ano Lectivo 2012/2013 Universidade de Lisboa Faculdade de Farmácia, Portugal
2010	Universidade de Lisboa/ Fundação Amadeu Dias Grant